LOCUS       LC550087               20358 bp    DNA     linear   BCT 04-AUG-2020
DEFINITION  Escherichia coli EHOUT50 genes for O-antigen biosynthesis gene
            cluster, complete cds.
ACCESSION   LC550087
VERSION     LC550087.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S. and Lee,K.
  TITLE     Additional O-genotyping PCR primers targeting novel E. coli and
            Shigella-unique O-genotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20358)
  AUTHORS   Iguchi,A., Iyoda,S. and Lee,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2020) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuenkibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..20358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EHOUT50"
                     /db_xref="taxon:562"
                     /note="Og48 variant O-antigen biosynthesis gene cluster"
     gene            103..906
                     /gene="wcaM"
     CDS             103..906
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BCG06397.1"
                     /translation="MAINDSDILISDHIIERINCTNGKINWGIGIGLAGSTYDNNYPE
                     DQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYG
                     CDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISA
                     GNAVSFVSLTNIEMKRASLELHNKPQHLFMRNIKVMQESSVGPALSMNFDMRKDVRGV
                     FMAKEETLLSLANVHAVNERGQSSVDIDRINHHIVNVEKINFRLPERRE"
     gene            1069..1974
                     /gene="galF"
     CDS             1069..1974
                     /gene="galF"
                     /note="OgN48v_1"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BCG06398.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPDDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            2346..3431
                     /gene="rmlB"
     CDS             2346..3431
                     /gene="rmlB"
                     /note="OgN48v_2"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BCG06399.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPHAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAENIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            3431..4330
                     /gene="rmlD"
     CDS             3431..4330
                     /gene="rmlD"
                     /note="OgN48v_3"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="BCG06400.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEANATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            4388..5266
                     /gene="rmlA"
     CDS             4388..5266
                     /gene="rmlA"
                     /note="OgN48v_4"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BCG06401.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDSAVNKESGATIFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGQYLLKMIKGY"
     gene            5271..5822
                     /gene="rmlC"
     CDS             5271..5822
                     /gene="rmlC"
                     /note="OgN48v_5"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="BCG06402.1"
                     /translation="MNVIKTEIPDVLIFEPKVFRDERGFFFESFNQKVFEDAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVT
                     LSAENKRQLWIPEGFAHGFITLSEFAEFLYKTTSFYSPKNEVCIRFDDEKIGIDWQCA
                     EYMKLSEKDSKGISLDEYVRTVV"
     CDS             5829..6743
                     /note="OgN48v_6"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="glycosyltransferase"
                     /protein_id="BCG06403.1"
                     /translation="MNKVSILMPVYNVEKYIRAAIASILNQTYPNIELVIVDDASTDN
                     TLLIIDEIAQKNNNIIIVRNDVNSGITVALNKGLKYCSGDYIARADGDDIQDPLRIQK
                     QIDFLKENQEYGLVGCWIKNIDESGKQIASCKYPVSDLEVRQCVSYTSPVLHIWLAKK
                     EVYDKLNGYRNTNPAEDYDFILRCLDYGIRIGNVPYYGSMIRLRSGSTLTESSLKQRV
                     ISNYLCKKFKKGIINTIDIESELPKTNELKIITKLHSWSCFFLKKAIENKRSLKGAAY
                     LCLSLISPFTVQNIIKRQIFKRKCNKGY"
     CDS             6745..7788
                     /note="OgN48v_7"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /product="glycosyltransferase"
                     /protein_id="BCG06404.1"
                     /translation="MIKIAIILPSLKNVGPNRVAHTLINKLSNKEGVYLKVFYIKNEL
                     GLNFTCDTELLTLVNVRKLYAFDVLHSHMLRPDVLVGLLPFYKGLKISTIHNIVTEDI
                     QYTHGSVISKIFSELWFRTWSRFDKIVVLSKVAKKYYLGHGLKSEKLEIIYNGIDLDI
                     GSNINYEDKKIIEEFKGDSTLLGTLCLANHRKGIEQILYALPFLPNYKFIIIGDGPVR
                     EYLENLAISLNVRERFFILGYRENAKDLLKYIDIYLMPSRSEGFGLALLEAVAAKITV
                     VCSNIPIFKEIFTERQVSFFELDNTNSLIQAIHNSINNDINDAYIRLKQNYSSDTMAR
                     LYHELYMTCKEVK"
     CDS             7785..8567
                     /note="OgN48v_8"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="glycosyltransferase"
                     /protein_id="BCG06405.1"
                     /translation="MRLFISIVSHGHGELIESLGCLEKLSEKFSVVLKDNKNESNLKE
                     YCKHNNITYLSSDIPRGFGENNNIIFNHCSSELGMQPDDIFVVMNPDLIIEENELIKL
                     KNKMMCENIDFATINLYKDRDYKIYDPSIRNYPGILDFVNSYLGFSNKTIINKSVIKS
                     NTPVPWAAGSFLALKSYLYKEVDGFNIRYFMYCEDIDICMRVGSYGYKLIYIPTIKAI
                     HIARHNNRRFLSKHFYWHLRSMLIYLSEKRKYVAKKNKCYTN"
     gene            8569..9762
                     /gene="wzx"
     CDS             8569..9762
                     /gene="wzx"
                     /note="OgN48v_9"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BCG06406.1"
                     /translation="MIGGNALAQIIVILGTPLLTRMYQPSDFGIYSTIMAVVLIVGII
                     ACGRYDQIMYNFNDKYSWYKCFCNGVVIATYISLFCLIVTSILFFLFKFDVTYFIIAP
                     SVLTFAILQLYTSYFSLYAYYRIIIILNIIRSSSLIVLQLMLFKVIENGLVVSFLISQ
                     IICVLFCLSFAGKLNKNFNFEFKVVFIKAREAGLSSVQSLANSFSSQLPVLFIPSQYG
                     YYTVGLYGLAVRLTQIPIIFFTNAVRPYILGELNKKKNDYRLLSKILWRSSALLLILS
                     ILGIVLINLFAEDFFKFYAGEEWKDAGKIASVLSWWLLIAFANVTSTSYLTVVGRFKS
                     LFIYDMLLLVFRLMSVCYSFYADLSFLNFIYIYSILGMFFNLGIIGYAIKCGIQDAKN
                     INCYS"
     CDS             9734..10759
                     /note="OgN48v_10"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /product="hypothtical protein"
                     /protein_id="BCG06407.1"
                     /translation="MQKILIVTAENGWFAQGKFPWESMDIERIKRRLESNNKIVNVTT
                     FQFLKDNIASLKDYIIIYTSSQRLEHKYYIQDIMFLLKDNNTLIPNYESLLAHENKGF
                     QSLLDKKYNLGLIKSEYYCDICEVKNIKNLNYPLVYKSVNGASSMGVKKVHNIEEMYK
                     YVKQPCSISLELLKRKLKKYIFKSKYNHQWEKYLSFAQQRFIIQGFIPDLQYDFKVLI
                     FGKKYYLLKRFVAENDFKASGSGLHSRNIGSDLKIVLDFARGIKNKFRSHIYSLDICV
                     KNKEPHLIEFQMTHVGPVTLSESDHYFIYNATTNNWEKVFSLSSLEDEFSNAVEEYIN
                     ESDSYSA"
     CDS             10734..11792
                     /note="OgN48v_11"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf12"
                     /product="glycosyltranslocase"
                     /protein_id="BCG06408.1"
                     /translation="MKVIHIVHNYYGFSGASLQAKNLARALNNYSPHIEQIFVTRCQC
                     CISKLNDHDQFKVIEIPISYKRLWHFFLLCKSIKPDVVHFHGADFGLLSICAILKIPV
                     YWKSTLLDSDDFYTLLNRGSLRKFKFWLLKFISINNTLTKQIYKVNRQYIPTTKLVTI
                     PNGVSISSCTHRNELKKEKIVLIISAIIPRKKIIEGIRFYKDNFENKGYKLLIIGPDN
                     EELDGFEANYVTKCKSLSNNNIHFLGKLKHQSVSEIMCRSQFLIHLSEREGMPNVVLE
                     ALASGVYPIIGEMGGLAYEIIENESYGFIFNNKNKFDLNKYTGVNIMGMDIIDKKYSF
                     DVISKKTVNIYTKLIGGK"
     gene            11789..12910
                     /gene="wzy"
     CDS             11789..12910
                     /gene="wzy"
                     /note="OgN48v_12"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BCG06409.1"
                     /translation="MKEESHDNDKGVSFIGFLWLLACGLYITRITAISPVYLVFILAL
                     MLAILTNVNCGKYKVSVFTLLSLIILLLSIFLFFNSQIPLVINLLIVLLSPMLVDSYY
                     KRRYITKQNLLNVFLFYSLVFILDGFWRIKHPYLEHVDKLEELGIGFQIYKVNSFMYS
                     DSNFVGLEAVFILSCFLYMFKSVRRTGLLRIKYFFVFCALFFGVLLTFSRAAIIAMIL
                     LFIFFILMRSKFIKTVSYMLAPVLLVVFSWFIYKRFTDDISFESKFNLIRYTLEYIKD
                     SDIDMILLGVGLGNAVEVIGMGAHNLFLTFLIETGVIGLLLFLVLLCIMWFYLKEDAI
                     IIMLPFLIASMSLGTTAIPYFFTFTWLCILYKRKLFDVV"
     gene            12929..13936
                     /gene="gne"
     CDS             12929..13936
                     /gene="gne"
                     /note="OgN48v_13"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BCG06410.1"
                     /translation="MNILVTGGLGYIGSHTLIRLIELKHNIIVVDNLSNSKASVIERI
                     KSLSGGDFIFYNCDICNAKCLQEIFSKHNINKVIHFAGLKSVSESGKAPLTYYHNNVV
                     GTLNLLKVMYSNGIKDIIFSSSATVYGVPDIIPLTEKCVTGGTTNPYGTSKHMVEQIL
                     MDLIKSDGDWNITILRYFNPIGAHPSGEIGEDPQGVPNNLVPYVAKVAIGDLPFLTIY
                     GDDYNTPDGTGIRDYIHVCDLAEGHIAALNNKEISGLHIYNLGTGVGSSVLDIINTFE
                     QVNHVSIPYRISKRREGDVAQCWADPTLIQTKLNWRAKYNLEDMLKHAWLWQLKNKGY
                     V"
     gene            14044..15450
                     /gene="gnd"
     CDS             14044..15450
                     /gene="gnd"
                     /note="OgN48v_14"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BCG06411.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKVGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            15672..16865
                     /gene="ugd"
     CDS             15672..16865
                     /gene="ugd"
                     /note="OgN48v_15"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BCG06412.1"
                     /translation="MFNRKKSELMKITISGTGYVGLSNGILIAQNHEVVALDIVQAKV
                     DMLNKKQSPIVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFN
                     TSSVEAVIRDVTEINPDAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYD
                     NLHPSRIVIGEQSDRAERFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVA
                     YFNELDSYAESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQS
                     VPNNLISAIVDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRI
                     KAKGVPVIVYEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRD
                     LFGND"
     gene            complement(16931..17935)
                     /gene="gla"
     CDS             complement(16931..17935)
                     /gene="gla"
                     /note="OgN48v_16"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase"
                     /protein_id="BCG06413.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVRHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPHWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            18287..19321
                     /gene="wzz"
     CDS             18287..19321
                     /gene="wzz"
                     /note="OgN48v_17"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BCG06414.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIENLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(19418..20029)
                     /gene="hisI"
     CDS             complement(19418..20029)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BCG06415.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDSLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRMAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     6160 a   3637 c   4399 g   6162 t
ORIGIN
        1 aattacgcca tcttacgcca gggatttcat aaccagatta tcggtgccaa catcaccaat
       61 tgtaagttca gcgacttaca gggggacgct attgaatgga acgtggcgat taacgacagt
      121 gatattttga tctccgacca catcatcgag cgcatcaact gtactaacgg caaaatcaac
      181 tggggcattg gcataggtct tgcgggaagc acttatgata ataactaccc ggaagaccag
      241 gcagtgaaaa actttgtcgt ggcgaatatc acggggtcgg attgtcggca gttgatccat
      301 gttgaaaatg gtaaacattt tgttattcgt aatatcaaag cccgcaatat cacgccggat
      361 ttcagtaaga aagcaggtat tgataacgcc acagtcgcta tttacggttg tgacaatttc
      421 gtgattgata atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt
      481 aaaggcaaat atctctcgat accgcaaaat ttccgagtga ataatattca gctggataac
      541 acccatcttg cttataaatt gcgcggtatc caaatctctg ccgggaatgc tgtctccttt
      601 gtgtcgctaa ctaacattga gatgaagcgt gcctcgctgg agttacacaa caagccgcaa
      661 catcttttta tgcgaaatat caaagtgatg caggaatcct ctgttggacc cgcattgagc
      721 atgaacttcg acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaga agaaacactg
      781 ctgtctcttg caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac
      841 aggattaatc accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg
      901 gagtagattt gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca
      961 atccacaacg aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat
     1021 gctggcgaaa aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata
     1081 atgacgaatt taaaagcagt tattcctgta gcgggtcttg ggatgcatat gttgcctgcc
     1141 actaaggcga ttcccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatt
     1201 gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag
     1261 aacgcggtcg aaaaccactt cgacacctct tatgaattag aatctcttct tgagcagcgc
     1321 gtgaagcgtc aactactggc ggaagtgcag tccatctgtc caccgggcgt gaccattatg
     1381 aacgtgcgtc agggcgaacc tttaggtttg ggccactcca ttttatgtgc acgacccgcc
     1441 attggtgaca atccatttgt cgtggtgctg ccagacgttg tgatcgacga cgccagcgcc
     1501 gacccgctgc gctacaacct tgctgccatg attgcgcgct tcaacgaaac gggccgcagc
     1561 caggtgctgg caaaacgtat gccggatgac ctctctgaat actccgttat tcagaccaaa
     1621 gagccgctgg atcgcgaagg taaagtcagc cgcatcgtgg aatttatcga aaaaccggat
     1681 cagccgcaga cgctggactc agacatcatg gccgtgggtc gctatgtgct ttctgccgat
     1741 atttggccgg aacttgaacg cactcagcct ggtgcatggg ggcgtattca gctgactgat
     1801 gccatcgctg aactggcgaa aaaacagtcc gttgatgcca tgctgatgac aggtgacagc
     1861 tacgactgcg gtaaaaaaat gggttatatg caggcatttg tgaagtatgg actacgcaac
     1921 ctgaaagaag gggcgaagtt ccggaaaggg attgagaagc tgttaagcga ataatgaaga
     1981 tctgaccgaa tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcgaaa
     2041 gtaatttgtt gcgaatattc ctgccgttgt tttatataaa caatcagaat aacaacgagt
     2101 tagcaatagg attttagtca aagttttcca ggattttcct tgtttccaga gcggattggt
     2161 aagacaatta gcgtttgaat tttttgggtt tagcgtgagt gggtaacgct cgtcacatcg
     2221 tagacatgca tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt gcattaatac
     2281 ctctattaat caaactaaga gccgcttatt tcacagcatg ctctgaagta atatggaata
     2341 aaagagtgaa gatacttgtt actggtggcg caggatttat tggttctgct gtagttcgtc
     2401 acattataaa taatacgcag gatagtgtta ttaatgtcga taaattaacg tacgccggaa
     2461 acctggaatc acttgctgat gtttctgatt ctgaacgcta tatttttgaa catgcggata
     2521 tttgcgatgc tgctgcaatg gcacggattt ttgctcagca tcagccgcat gcagtgatgc
     2581 acttggctgc tgaaagccat gttgaccgtt caattacagg tcctgcggca tttattgaaa
     2641 ccaatattgt tggtacttat gtccttttgg aagccgctcg caattactgg tctgctcttg
     2701 atagcgacaa gaaaaatagc ttccgttttc atcatatttc tactgacgaa gtatatggtg
     2761 atttgcctca tcctgacgag gtaaataata cagaagaatt acccttattt actgagacaa
     2821 cagcttacgc gccaagcagc ccttattccg catccaaagc atccagcgat catttagtcc
     2881 gcgcgtggaa acgtacctat ggtttaccga ccattgtgac taattgctct aacaattatg
     2941 gtccttatca tttcccggaa aaattgattc cattggttat tctcaatgct ctggaaggta
     3001 aagcattacc tatttatggt aaaggggatc aaattcgcga ctggctgtat gttgaagatc
     3061 atgcgcgtgc gttatatacc gtcgtaaccg aaggtaaagc gggtgaaact tataacattg
     3121 gtggacacaa cgaaaagaaa aacatcgatg tagtgctcac tatttgtgat ttgttggatg
     3181 agattgtccc gaaagagaaa tcttaccgcg agcaaattac ttatgttgcc gatcgtccgg
     3241 gacacgatcg ccgttatgcg attgatgctg agaacattgg tcgcgaattg ggatggaaac
     3301 cacaggaaac gtttgagagc gggattcgta aaacggtgga atggtacctg tccaatacaa
     3361 aatgggttga taatgtgaaa agtggtgcct atcaatcgtg gattgaacag aactatgagg
     3421 gccgccagta atgaatatcc tcctttttgg caaaacaggg caggtaggtt gggaactaca
     3481 gcgtgctctg gcacctctgg gtaatttgat tgctcttgat gttcactcca ctgattactg
     3541 tggtgatttt agtaatcctg aaggtgtagc tgaaaccgta agaagcattc ggcctgatat
     3601 tattgtcaac gcagccgctc acaccgcagt agacaaagca gaatcagaac cggagtttgc
     3661 acaattactt aacgcgacga gtgtcgaagc gatcgcgaaa gcagccaatg aagtcggcgc
     3721 ctgggttatt cactactcta ctgactacgt atttccgggg accggtgaaa taccatggca
     3781 ggaggcgaat gcaaccgcac cgctaaatgt ttacggtgaa accaagttag ctggagaaaa
     3841 agcattacaa gagcattgtg cgaagcacct aattttccgt acaagctggg tctatgcagg
     3901 taaaggaaat aacttcgcca aaacgatgtt gcgtctggca aaagagcgtg aagaattagc
     3961 cgttattaat gatcagtttg gtgcgccaac aggtgctgaa ctgctggctg attgtacggc
     4021 acatgcaatt cgtgtggcac tgaataaacc agaagttgca ggcttgtatc atctggtagc
     4081 cagtggtacc acaacctggc acgattatgc tgcgctggtt tttgaagagg cgcgcaaagc
     4141 aggcattccc cttgcactca acaagctcaa tgcagtacca acaacagcct atcctacacc
     4201 agcccgtcgc ccccataact ctcgtcttaa tacagaaaaa tttcagcaga actttgcgct
     4261 tgtcttgcct gactggcagg ttggtgtgaa acgaatgctc aacgaattat ttacgactac
     4321 agcaatttaa tagtttttgc atcttgttcg tgatggtgga gcaagatgaa ttaaaaggaa
     4381 tgatgaaatg aaaacgcgta aaggtattat tttagcgggt ggttctggta cacgtcttta
     4441 tcctgtgact atggctgtca gtaaacagct attacctatt tatgataagc cgatgatcta
     4501 ttacccgctt tctacactga tgttagcggg tattcgcgat attctgatta tcagtacgcc
     4561 gcaggatact cctcgttttc aacaactgct gggtgacggg agccagtggg ggctaaatct
     4621 tcagtataaa gtgcaaccga gtccagatgg acttgcacag gcatttatca tcggtgaaga
     4681 gtttattggt ggtgatgatt gtgctttggt acttggtgat aatatcttct acggtcacga
     4741 cctgccgaag ttaatggact cagctgttaa caaagaaagt ggtgcaacga tatttgccta
     4801 tcacgtaaat gatcctgaac gctatggtgt cgttgagttt gataaaaacg gtactgcaat
     4861 cagcctggaa gaaaaaccgc tacaaccaaa aagtaattat gcggtaaccg ggctttattt
     4921 ctatgataac gacgttgtcg aaatggcgaa aaaccttaag ccatctgccc gtggtgaact
     4981 ggaaattact gatattaacc gtatttatat ggaacagggg cgtttgtctg ttgccatgat
     5041 ggggcgtgga tatgcatggc tggacacggg aacacatcaa agcttgattg aggcaagcaa
     5101 cttcattgct acaattgaag agcgtcaagg gttgaaagta tcctgtccgg aagaaattgc
     5161 ttaccgtaaa gggtttattg acgcagagca ggtgaaagca ttagctgagc cgctgaaaaa
     5221 aaatgcttat ggtcagtatc tgctgaaaat gattaaaggt tattaataaa atgaacgtaa
     5281 ttaaaacaga aattccagat gtactgattt ttgaaccgaa agtttttagg gatgagcgcg
     5341 gtttcttttt cgagagcttt aaccaaaagg tttttgagga tgcggtaggt cgcaaagttg
     5401 agtttgtcca ggataatcat tcgaagtcat gcaaaggggt tttacgtgga ctgcattatc
     5461 agttagaacc ttatgcacaa ggaaaattgg tgcgctgtgt tgttggtgag gtttttgatg
     5521 ttgcggtgga tattcgtaag tcttcgccta cgtttggtaa atgggttggg gtgactttat
     5581 ctgctgagaa taagcgtcag ttgtggatcc ctgaaggatt tgcacatgga ttcataacat
     5641 tgtcagagtt tgcagagttt ctttataaaa caactagttt ttattcccca aaaaatgaag
     5701 tttgtatccg gtttgatgat gaaaaaatag ggatagattg gcagtgtgca gagtatatga
     5761 aactctcaga aaaagattca aaagggatat ctctcgatga atacgtgcgc acggtggtat
     5821 gatagattat gaacaaagtt agcatattaa tgcctgttta taatgtggaa aaatatatta
     5881 gagctgcgat tgctagtata ttaaaccaaa catatccgaa tatagagtta gtaattgtag
     5941 acgatgcgtc tactgacaat acattactca ttatagatga aattgcgcaa aaaaataata
     6001 acataattat tgttcgcaat gatgtaaact ctggaataac cgtagcgcta aacaaaggtc
     6061 ttaaatattg tagcggtgat tatatagctc gagctgatgg tgatgacata caagatcctt
     6121 tacgaattca aaagcaaata gactttctaa aggagaatca agaatatgga ttagttggat
     6181 gttggataaa aaacatagat gagtcaggaa aacaaatagc ttcatgtaaa tatccggttt
     6241 cagacttaga agtgagacaa tgcgttagct atacatctcc tgtgcttcat atatggttag
     6301 caaagaaaga agtttacgat aaattgaatg gatacaggaa tacaaatcca gctgaagatt
     6361 atgattttat tttaagatgt ttggattatg gtataagaat agggaacgtt ccgtattatg
     6421 gttcaatgat acgtttacgt tcagggagta cattaactga atcttcttta aaacaacggg
     6481 ttatatcaaa ctatctatgt aaaaaattta aaaaaggcat tattaataca attgatatcg
     6541 aatctgaact acctaaaaca aatgaactta aaattattac taaattgcat tcttggtcat
     6601 gtttcttttt aaaaaaagca atagagaaca aaaggtcgtt aaaaggggca gcttatttat
     6661 gtttgtcgct tataagtcct tttacagttc aaaatataat aaaaaggcag atttttaaaa
     6721 gaaaatgtaa caagggttat taaattgata aaaatagcaa taatattacc atcattgaaa
     6781 aacgttggac cgaatagagt tgctcatact cttattaaca aactgagtaa taaagaaggt
     6841 gtatacctaa aggtttttta tataaaaaat gaattaggat taaattttac ttgtgataca
     6901 gaacttttga ctttagttaa tgtacgaaaa ttatatgcct tcgatgtttt gcattcacat
     6961 atgcttaggc cagatgtctt ggttgggcta cttccatttt acaaaggcct taaaataagc
     7021 acaatccata atatagtaac agaagatata caatatacac atggtagtgt gatatctaaa
     7081 attttctctg aattatggtt taggacttgg agtagatttg ataagatagt cgtgctatca
     7141 aaagtagcaa aaaaatacta tctgggacat gggctaaaat ctgaaaaatt agagatcata
     7201 tataatggaa ttgatctgga tataggaagt aatatcaatt atgaagataa gaaaataata
     7261 gaggagttta aaggagacag tactcttctg ggaacacttt gtttagcaaa tcacaggaaa
     7321 ggtatagaac agattcttta tgccttgcca ttcttaccta attataaatt tataattatt
     7381 ggtgatggac ctgtaagaga atatttagag aatttagcta taagtctgaa tgtaagagag
     7441 cgatttttta ttctgggcta tagagaaaac gctaaagatt tattaaaata tatcgatatt
     7501 tatctaatgc caagtagatc ggaaggattt ggtctcgcat tgcttgaggc tgttgcagca
     7561 aaaataacag tagtttgctc aaatattcca atatttaaag aaatctttac tgaacgacaa
     7621 gtgtcttttt ttgaattgga taatacaaac tcacttattc aagcgataca taactcaata
     7681 aataatgata taaatgacgc gtatataagg ctaaaacaaa attatagctc agatacgatg
     7741 gctcgattat atcatgaatt atatatgact tgtaaggaag taaaatgagg cttttcatat
     7801 caattgtatc acatggacac ggggaattaa ttgagtcatt agggtgttta gaaaaacttt
     7861 cggaaaaatt tagtgttgtg cttaaagata ataaaaatga gtccaacctt aaagagtatt
     7921 gcaaacataa taatataacc tacttaagta gcgatattcc tagaggattt ggggagaata
     7981 ataatattat atttaaccac tgctcatcag aattaggcat gcaaccagat gatatatttg
     8041 tagtaatgaa tccagatctg attattgaag aaaatgaatt gataaaactt aaaaataaga
     8101 tgatgtgtga aaatatagac tttgccacta taaatcttta taaagatagg gactataaaa
     8161 tatatgatcc atcaatcagg aattatccgg gcattcttga ctttgtaaat agttatcttg
     8221 gtttctcaaa taaaacaata ataaataaat cggtaattaa atcaaatacc ccggtgccat
     8281 gggctgcagg ttcattcttg gcattaaagt cttatctcta taaagaagtt gatggtttta
     8341 atatcagata tttcatgtat tgcgaagata tagatatatg tatgagggta ggttcttatg
     8401 gttataaatt aatttatata ccaacaataa aagccataca tattgcacgg cacaataatc
     8461 gacgtttttt atcaaaacat ttttattggc atttacgtag tatgcttata tatctatcgg
     8521 aaaaaagaaa gtatgtcgct aaaaaaaata agtgctacac taattaaagt gatcggtgga
     8581 aatgcattag ctcagattat tgttatatta ggcactcctt tacttacgag aatgtaccaa
     8641 ccgagtgatt ttggtatata ttcgaccatt atggcagtag ttttaatagt gggaattatt
     8701 gcatgtggta gatatgacca gataatgtat aacttcaatg acaaatattc ttggtataaa
     8761 tgtttttgca acggggttgt tatagcaaca tatatatctc tgttctgctt aattgtaacc
     8821 agtatactgt tttttttatt taaatttgat gtgacttact ttataattgc accttcagtg
     8881 ttgacatttg caattttaca gttgtataca tcttatttta gcttatatgc ttattacaga
     8941 ataataataa tattaaatat cattcgttct tcaagtttaa ttgtattaca attgatgttg
     9001 tttaaagtta ttgaaaatgg acttgtcgtg agctttctaa tatcgcaaat aatatgcgtt
     9061 ttgttttgct taagcttcgc ggggaaactg aacaaaaatt ttaattttga atttaaagtg
     9121 gtttttatta aagctagaga agctggattg agttctgtac aatctcttgc taattccttt
     9181 tcatcacaat tacctgttct ttttattcct agccaatatg gatattatac cgttggtcta
     9241 tatggattag ctgtgaggtt gactcagata ccaattattt tttttacaaa tgcagttagg
     9301 ccatatatat taggagaatt aaataaaaag aaaaatgatt atcgtctttt atcaaaaatc
     9361 ctatggagaa gttcggcttt attattgatt ttaagtattc ttgggatagt tcttattaat
     9421 ttatttgcgg aagatttttt taagttttat gctggtgaag aatggaaaga cgcaggtaaa
     9481 atagcctctg ttttgtcatg gtggctatta attgcattcg ctaatgttac gtcgacttca
     9541 tatctaactg ttgtagggcg atttaaatca ttatttatct atgatatgct tctattggta
     9601 ttcagattaa tgtcagtatg ctatagtttt tatgcggatt tatcattctt aaactttata
     9661 tatatttatt caatacttgg tatgtttttt aatctaggta ttataggtta tgcaataaaa
     9721 tgtggaattc aagatgcaaa aaatattaat tgttacagct gagaacggtt ggtttgcgca
     9781 gggtaaattc ccttgggaaa gtatggatat tgaaaggata aagagaaggt tggaaagtaa
     9841 taataaaatt gttaatgtga ctacttttca atttctcaaa gataatattg cctcgctgaa
     9901 agattacata ataatatata catcatctca acgactggaa cataaatatt acatacaaga
     9961 tataatgttc ttattaaaag ataacaatac tcttatccct aactatgaat cacttttggc
    10021 gcatgaaaac aaaggatttc aaagtttact tgataagaaa tataatttgg gtttaataaa
    10081 atctgaatat tattgcgata tttgtgaagt aaagaatatt aaaaatttga attatcccct
    10141 cgtttataaa tctgtgaatg gcgcatcatc aatgggggta aaaaaagtgc acaacataga
    10201 agaaatgtat aaatatgtta aacaaccttg ctcaatttca ttggaattat tgaaaagaaa
    10261 actaaaaaaa tatattttta aaagcaaata taatcatcag tgggagaaat atctgtcatt
    10321 tgcgcaacaa agatttatta ttcaaggttt cattcctgat ttacaatatg attttaaagt
    10381 gttgattttt ggtaaaaaat attatctctt aaagaggttc gtcgctgaga atgattttaa
    10441 agctagtggc agtgggttgc actcgagaaa tattggtagt gatttgaaaa tcgtgttaga
    10501 tttcgcaaga ggtattaaaa ataaatttag atcgcacata tattctttag atatttgtgt
    10561 gaaaaataaa gaaccacacc ttattgagtt tcaaatgacg cacgttggtc ctgttacgtt
    10621 gagtgaatct gatcactatt ttatttacaa cgcaacaaca aataactggg aaaaggtgtt
    10681 ttctttaagc tctcttgaag atgaattttc taatgcggta gaagagtata taaatgaaag
    10741 tgattcatat agtgcataat tattatggat ttagcggtgc atctcttcag gcaaaaaatt
    10801 tagcaagggc tttgaataat tatagtccgc atattgaaca aatatttgtg actagatgtc
    10861 agtgttgtat ttcaaaatta aatgaccatg atcaatttaa ggttatagag ataccgatat
    10921 catataaacg actttggcat ttttttctcc tgtgtaaatc aattaagccg gatgttgtac
    10981 attttcatgg ggctgatttc ggtctgctaa gtatttgtgc tattctaaaa ataccagttt
    11041 attggaaatc aactctatta gatagtgatg atttttatac attattgaat cgtggttctt
    11101 tgaggaaatt taagttttgg ttgttgaagt tcatctccat aaataacacg ttaactaagc
    11161 aaatatataa agttaaccgc caatatattc ctacaactaa attagttacg attccaaatg
    11221 gtgtaagcat ttctagttgt acacacagga atgagctgaa aaaagaaaaa attgttttaa
    11281 taatatctgc aatcatacca agaaaaaaga ttatcgaagg gataagattt tataaagata
    11341 actttgaaaa caaaggatat aaacttttga taattggccc tgataatgaa gaacttgatg
    11401 gtttcgaagc aaactacgta acgaaatgta agagtttaag taataataat atccattttt
    11461 taggtaaatt gaaacatcaa agcgtaagtg aaataatgtg tcgctctcaa tttttaatcc
    11521 atttatctga gcgagaaggc atgccaaacg ttgtattgga ggcactggcc agtggtgtct
    11581 atccgattat aggagaaatg ggtggccttg catatgagat tatagaaaat gaatcgtatg
    11641 gttttatttt taataataaa aataagtttg atttaaataa atacactggt gttaatataa
    11701 tggggatgga tataattgat aaaaaatatt cattcgatgt gatttcaaaa aaaacagtta
    11761 atatatatac gaaacttatt ggtggaaaat gaaagaagaa agtcatgata atgataaagg
    11821 agtctctttt attggttttt tatggctact ggcatgtggg ttgtatataa caaggataac
    11881 tgctattagt cctgtttatc tggtttttat tctcgcatta atgttagcta ttcttacaaa
    11941 tgttaactgc gggaaatata aggtttccgt ttttacacta ctaagtttaa taatactatt
    12001 actttcaata tttctttttt ttaattcaca aatacctctc gtaattaatc tgttgatagt
    12061 gttactatcg ccaatgctag tggattctta ttataagagg cgatatatta caaaacaaaa
    12121 tttattgaat gtgtttttat tttattcctt ggttttcatt cttgatggtt tttggcgaat
    12181 taaacatccc tatcttgaac atgtagataa actagaagaa ctgggtatcg gctttcaaat
    12241 atataaagtg aattcattca tgtattcaga ttctaatttt gttggcctgg aagctgtttt
    12301 tatactatct tgttttcttt acatgtttaa aagtgttcga cggactggat tacttcggat
    12361 aaaatatttt ttcgtttttt gtgctttatt ttttggggtt ctgttaactt tttctagggc
    12421 tgctatcata gcaatgattt tactttttat atttttcatt ttgatgagga gtaaatttat
    12481 taaaactgtt tcgtatatgt tagcgccagt attacttgtg gttttttcat ggtttatata
    12541 taagagattt acggatgata taagttttga gagtaaattt aatttaatta ggtataccct
    12601 tgaatacatt aaagatagtg atattgatat gatattgcta ggtgttggtc tgggaaatgc
    12661 agtcgaagta attggtatgg gggcacataa tctatttttg actttcttaa tcgaaactgg
    12721 tgttattggt ttattattgt ttttagtttt attatgcatt atgtggttct atctcaaaga
    12781 agatgcaatc atcataatgc ttccatttct aatagcatca atgtcgctcg ggacaaccgc
    12841 aataccttac ttttttacct ttacttggtt atgtattctt tacaaaagaa aattgtttga
    12901 tgtcgtctga cattggatag gaaacagtat gaatatttta gttaccggtg gcttaggata
    12961 tataggctct catactttaa ttcgattgat tgagcttaag cataacatca tagtggttga
    13021 taacctttca aactctaaag caagtgtgat cgagcgaata aaatcactga gtggtggaga
    13081 ctttatattt tataactgtg atatttgtaa tgctaagtgt ctgcaagaga tattctctaa
    13141 acataatatc aataaagtca ttcattttgc cggattgaaa tcagtttcag agtcaggtaa
    13201 ggcacctcta acatattatc acaataatgt agttggaacg ttaaatttat tgaaggtaat
    13261 gtattcaaat ggaataaagg atattatatt tagttcttct gcaactgtat acggggtgcc
    13321 agatattata cctttgactg aaaaatgcgt aactggagga actacaaatc cttatggaac
    13381 cagtaaacac atggtcgagc aaatcttaat ggatttaatt aagtctgatg gcgattggaa
    13441 tataacaatt cttcgatatt ttaatcctat tggagcccat ccttcaggag agattgggga
    13501 ggacccgcaa ggagtaccta ataatttagt accttacgta gctaaagtcg caattggaga
    13561 tttgcctttt ttaaccattt atggcgatga ttacaatact cctgatggaa caggaatcag
    13621 agattatatt catgtttgtg acttagctga agggcatata gctgcattga acaataaaga
    13681 aattagtggt ttgcatatct ataatttagg cacaggcgtt ggctcatctg tattagatat
    13741 aataaataca tttgagcaag ttaatcatgt ttcgattcct tatcgtatat ctaaaaggcg
    13801 agagggagat gtcgcgcaat gctgggctga cccaacattg attcaaacta agttaaattg
    13861 gcgtgcaaaa tataatcttg aggacatgct taagcatgct tggctttggc aactcaaaaa
    13921 caagggatat gtttaattga ggggatactt ttatttgtgc attaattttt cagttacaaa
    13981 gatgtatgct tacatcattt gaaatgtcat aatcaaagca gtgtcccctg acaggagtaa
    14041 acaatgtcaa agcaacagat cggcgtcgtc ggtatggcag tgatggggcg caaccttgcg
    14101 ctcaacatcg aaagccgtgg ttataccgtc tctattttca accgttcccg tgaaaagacc
    14161 gaagaagtga ttgccgaaaa tccaggcaag aaactggttc cttactatac ggtgaaagag
    14221 tttgttgaat ctctggaaac gcctcgtcgc atcctgttaa tggtgaaagc aggtgcaggc
    14281 acggatgctg ctattgattc tctcaagcca tacctcgata aaggtgacat catcattgat
    14341 ggtggtaata ctttcttcca ggacaccatt cgtcgtaacc gcgagctttc tgccgaaggc
    14401 tttaacttta tcggtaccgg tgtttccggt ggtgaagaag gtgcgctgaa aggtccttcc
    14461 attatgcctg gtgggcagaa agaagcctat gagctggttg caccaatcct gaccaaaatc
    14521 gccgcagtgg ctgaagacgg tgagccatgc gttacctata ttggtgccga tggcgcaggt
    14581 cactatgtga agatggttca caacggtatt gaatatggtg atatgcagct gattgccgaa
    14641 gcctattctc tgctaaaagt tggcctgaac ctctccaacg aagaactggc ccagaccttt
    14701 accgagtgga ataacggtga actgagcagc tacctgatcg acatcaccaa agatatcttc
    14761 accaaaaaag atgaagacgg taactacctg gttgatgtga ttctggatga agcagcaaac
    14821 aaaggtacgg gcaaatggac cagccagagt gcgcttgatc tcggcgaacc gctgtcgctg
    14881 attaccgagt ctgtgtttgc acgttatatc tcttctctga aagatcagcg tgttgccgca
    14941 tctaaagttc tctctggccc gcaagcacag ccagcaggcg acaaggctga gttcatcgaa
    15001 aaagttcgcc gtgcgctgta tctgggcaaa atcgtttctt acgctcaggg cttctctcag
    15061 ctacgcgccg cgtctgaaga gtacaactgg gatctgaact atggtgaaat cgcgaagatt
    15121 ttccgtgctg gttgcatcat ccgtgcgcag ttcctgcaga aaatcaccga tgcgtatgcc
    15181 gaaaatccgc agatcgctaa cctgctgctg gctccttact ttaagcaaat tgccgatgac
    15241 taccagcagg cgctgcgtga tgtcgttgct tatgcggtac agaacggtat cccggttccc
    15301 accttctctg cggcagttgc ctattatgac agctaccgtg ccgctgttct gcctgcgaac
    15361 ctgatccagg cacagcgtga ctatttcggt gcgcatactt ataagcgcat tgataaagaa
    15421 ggtgtgttcc ataccgaatg gctggattaa tctgatttaa atcaattaat caatgcaagg
    15481 cccggagaaa ccctccgggc ttttttatta tacaaagcgg caggttatgg ccttttttta
    15541 taatttatag ttaaaaacgc gatataatac agcgccgcac agcaggatcg ctgccttgac
    15601 agttcatcta catcagcgtt aaaaatcccg cagtagatga agttgtggtg gtgaattaat
    15661 gaccactcta aatgtttaac cggaagaagt cagagctaat gaaaataaca atttcaggaa
    15721 caggttatgt tggtctttca aatggtattc tgattgcgca aaatcacgaa gtggttgcac
    15781 tggatatcgt tcaggccaaa gtggacatgc ttaacaagaa gcagtcaccg attgttgata
    15841 aggagattga agagtatctg gcgactaaag atctcaattt ccgcgctacg acagataagt
    15901 atgacgcgta taaaaatgcc gattacgtta ttattgccac acctaccgat tatgatccga
    15961 aaacaaatta ttttaatacc tcaagcgtgg aagcggtcat tcgcgatgtt acagaaatta
    16021 atcccgacgc tgtaatgatt ataaaatcaa ctatccctgt tggttttaca gagtcaatta
    16081 aagaacggtt tggtattgaa aatgtgatct tttcgccgga gtttttacgc gaagggaaag
    16141 cactttatga taacttacac ccatcacgca ttgtgattgg cgagcaatcc gaccgcgcag
    16201 aacgttttgc agcgttatta caggaaggcg ctattaagca agacatacca acattgttta
    16261 ctgactcaac cgaggctgag gcgattaaac tttttgccaa cacctatctg gcgatgcgtg
    16321 tagcgtattt caatgaactt gatagttatg ctgaaagcct gggacttaat tcacgccaga
    16381 ttattgaggg cgtatgcctt gacccgcgta tcggtaatca ctacaacaac ccgtcattcg
    16441 gttatggtgg ttattgtctg ccgaaagata ctaagcagtt actggcaaat taccagtctg
    16501 tgccgaataa cctgatctcg gcaattgttg acgccaaccg cacgcgcaaa gattttattg
    16561 ccgattctat ccttgcacgt aaaccgaaag ttgttggcgt ctatcgtttg attatgaaga
    16621 gtggttcaga caatttccgt gcatcctcga ttcagggtat tatgaagcga atcaaggcga
    16681 aaggcgtgcc tgtaatcgtt tatgagccag ctatgaaaga ggacgatttt ttccggtcgc
    16741 gcgtggtacg tgatctggat gcgttcaaac aagaagctga tgttattatt tctaaccgta
    16801 tgtctgccga tctggctgat gtagcagata aagtttatac gcgcgacttg tttggcaatg
    16861 attaattatt ttgttttatt ctaagaaaag gccctaatat attagggcct tttcttatgg
    16921 ttttgtaaaa tcaaacttta tagaagttac gataccattc tacaaagttc tttaccccct
    16981 ctttaactga cgtttcaggt ttgaatccta ttacgtcata cagtgctttt gtatcagcac
    17041 tggtttccag tacatcaccg ggttggagag gcatcatatt cttgttggct tcaataccca
    17101 gagcctcttc taaggcattg atatagtcca tcaactccac aggcgaacta ttaccaatgt
    17161 tatagacacg atatggtgct gaacttgttg ctggcgagcc tgtttctaca gcccagtgtg
    17221 gatctttttc tggaataaca tcctgtaagc gaataatagc ttcggcaata tcatcaatat
    17281 aagtaaagtc acgcttcatt ttgccgaagt tgtaaacatc aatgctttta ccttccagca
    17341 tggctttagt aaatttaaat aatgccatat ccggacgtcc ccatggacca taaaccgtaa
    17401 agaaacgcag ccctgtggtc ggtaagccat acaaatgaga atacgtatgg gccatgagtt
    17461 cattcgcttt tttagttgct gcataaagcg aaacaggatg atctacagag tcatctgtag
    17521 agaaaggcat cttgcggttc atgccataaa cagaactgga ggaagcgtaa agtagatgcc
    17581 gaacattatt atggcgacat ccttccagta tgttcaggaa tccaatcagg tttgcatctg
    17641 catatgcatt gggattttca agggagtaac gtacaccggc ttgcgcagcg aggtttatta
    17701 cgcgatcgaa ccgctcgtct gcaaacagtg ccgccatttt ctcacgatcg gccaggtcaa
    17761 ttttataaaa actgaagttg tcgtgcttga gtaaatcaag tcgtgcttgt ttgaggttga
    17821 catcgtaata atcatttaag ttgtcaatgc ctacaacctg atgaccagct gcaagaagcc
    17881 gtttacttac atagaaaccg ataaagccag cagctcccgt aaccagaaat ttcatttata
    17941 atcctcgctc aggctagaat atagccaatc ttcatctggc ataactgaaa gttaaatcat
    18001 accgttagac aagaaaaaaa gataatcggt atcagttcta aacttggctg ttttttctgg
    18061 taacgtgctc attttacaat caaagctgtt ctaagctgac tatacaagcc gacgtcatta
    18121 tctccaaccg tatggcagaa gagcttaagg atgtggcaga caaagtctac acccgcgatc
    18181 tctttggcag tgactaacat cctgttatca gggctatttt cgccctgatt ctcttatgtt
    18241 ccctttgtaa taattcatta tttttatcat ttatcctata gcattcatgg cgattatcgc
    18301 taaactatgg cgacttaaaa ttatttcgtc cgttagggta atgatgagag tagaaaataa
    18361 taatgtttct gggcaaaacc atgacccgga acagattgat ttgattgatt tactggtgca
    18421 gttgtggcgc ggcaagatga caattatcat ctctgtcatt gttgctattg ccctggctat
    18481 agggtatctg gccgttgcca aagaaaagtg gacttccaca gctatcgtta ctcaacctga
    18541 tgtggggcaa atcgctggtt ataccaacgc gatgaatgtg atctacggtt cggctgtacc
    18601 gaaagtctcc gacattcagg cttcacttat cggacgttac agcactgcat tctcagcatt
    18661 agcggaaacg ctggataacc aggaagaagc agagaagctg accattgagc ctaccgttaa
    18721 aaaccagtcg ttaccattgg cggtgtctta tgttggtgaa acacctgaag gtgcgcagaa
    18781 gcagctggcg aagtatatcc agcaagttga cgaccaggta aacgaagaac tggaaaaaga
    18841 cctgaaagac aacatcgcgc tgcgtatgaa aaacttgcag gattctctga agacccagga
    18901 agtggttgcg caggagcaga aagatctgcg tatccgtcag attcaggaag cgttgcagta
    18961 tgcgaatcag gcgcaggtga caaagccgca gattcagcag acacaggacg taacccaaga
    19021 tactatgttc cttttgggga gcgaagcgct ggagtcgatg attaagcatg aggcgacccg
    19081 tccgttggtg ttctcaccaa actactatca gactcgtcaa aacctgctgg atatcgaaaa
    19141 cttaaaggtt gatgatcttg atattcatgc ttaccgctat gtaatgaaac cgacgttacc
    19201 tattcgtcgc gatagtccga aaaaggcgat caccttgatt ctggcggtgc tgctgggcgg
    19261 catggttggc gcggggattg tcttggggcg taacgctctg cgtaattaca acgcgaagta
    19321 atcttttcgg ttttaaagaa aaagggcagg gtggtgacac cttgcccgtt ttttttgccg
    19381 gatgcgacaa caacattgca tccgcttacc ccgcaactca ctgatgccgt ttacgcaggt
    19441 tctcaattac cgtcgttaaa tccagatcct gatcctgcaa cagcaccagc aggtgataca
    19501 tcaaatcaga cgcctcgttg gtgagctcaa agcggtcatg tactgtcgct gccagtgcgg
    19561 tttccacgcc ttcttcgccc actttctgcg ccatgcgttt ggtgccgctg gcatacagtt
    19621 tggcagtgta agaggtttcc gggtcggcag atttacgttc ggcgagcagt tgttccagtt
    19681 gataaaggaa cagccactgg tgagcggtgt cgccgaagca gctgctggtg cctttgtggc
    19741 aggtcggacc gatgggattc gccagcacta ataaactgtc gttgtcgcag tctggtgcaa
    19801 tactcaccac attgaggaaa ttgcccgacg tttcgccttt ggtccacagt cgctgtttag
    19861 tgcgcgagaa gaaggtgact ttgccgcttt cgatggtttt gtctaaggct tccgggttca
    19921 tatagcccag cattaacact tcaccggata ccgcgtgttg cacaatcacc ggcataagtc
    19981 cgtcggtttt ttcccagtcc agttcgcgac gttgttgttc tgttaacata tcctgatctc
    20041 cacgccctgt gttgccaggt acgcttttaa ttcaccaata ttgattattt gtttgtggaa
    20101 tacggaagct gccagcgcgc cgtcaacgtc ggcatcgcgg aaggcttcga ggaagtgttc
    20161 catggtgccc gcgccaccgg aagcaatcag cgggacgtgg caaacttcac gcactttttt
    20221 cagttgttcg aggtcgtaac cgttacgcac gccgtcctga ttcatcatgt tgaggacgat
    20281 ttctccggca ccgcgttttt gcacttcctg tacccagtcg agtgtttccc actgagtgac
    20341 gcgggtgcgg ctttcatc
//
