LOCUS       MN172354               13955 bp    DNA     linear   BCT 24-AUG-2019
DEFINITION  Escherichia coli strain 16-04846 O-antigen biosynthesis gene
            cluster, complete sequence.
ACCESSION   MN172354
VERSION     MN172354.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13955)
  AUTHORS   Lang,C., Hiller,M., Konrad,R., Fruth,A. and Flieger,A.
  TITLE     Whole genome-based public health surveillance of less common STEC
            serovars and untypable strains identifies four novel O genotypes
  JOURNAL   J. Clin. Microbiol. (2019) In press
   PUBMED   31366691
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13955)
  AUTHORS   Lang,C., Hiller,M., Konrad,R., Fruth,A. and Flieger,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2019) Division of Enteropathogenic Bacteria and
            Legionella (FG11), Robert Koch Institute, Burgstrasse 37,
            Wernigerode, Saxony-Anhalt 38855, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: A5 v. 2.1.3
            Assembly Name         :: OgN-RKI
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="16-04846"
                     /isolation_source="stool"
                     /db_xref="taxon:562"
                     /country="Germany"
     misc_feature    1..13955
                     /note="O-antigen biosynthesis gene cluster"
     gene            161..1054
                     /gene="galF"
     CDS             161..1054
                     /gene="galF"
                     /codon_start=1
                     /transl_table=11
                     /product="GalF"
                     /protein_id="QEE84098.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            1356..2606
                     /gene="wzx"
     CDS             1356..2606
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="QEE84092.1"
                     /translation="MYSNNLKKLVTHPVLLNIYWLMSEKILTVGLIFYAEGLIAKLLT
                     KYQYGEWIYSLNLVTLISSLALIAGAEVAVPALSRNKSLTDEIITSLFKIRFFFALIA
                     FLALNVYCVIFVEPSLLKKFIMMLSVIIILNEPFSVVINYYQSITKIKSIALLRLFSL
                     ACRTFFIWLCYCLTLQANLLALSRVIELFVLAISLIFLWKYKKFRWKVKPGITKFLFI
                     RGASLWPSLVLMYLYLRMDRFFVEHYLSFEVLAVYGIAVQLTEQTFVLVKMIIQSAAP
                     RYVYARMNHANLLRNIKKLILFNVIICSSISVLCFFVLPLIVTSLFGENYQRAASLAI
                     NMLPALFFYSIDSVIMQYLYREKKSILILFKWGAGTILMFVSYYMYFDFFEMKVLSII
                     YNLNYFCMMLLTITVFLWGRQYDK"
     CDS             2596..3420
                     /codon_start=1
                     /transl_table=11
                     /gene="orf3"
                     /product="glycotransferase"
                     /protein_id="QEE84093.1"
                     /translation="MISKKIVIFTPTYNRKHTLERCYQSIIQQDYPGVVWLVIDDGST
                     DNTEQLVTEFIKEGKLEIQYIYQTNGGKQSAWNKAVQLCQKYDLFICVDSDDILYENA
                     LTKVSKYFSLVDEDDVIGLRCLAIRNTTKKADSKFMNNKIHKDYWFMEIMSNQIGERV
                     DIFKPQKLLDYLFPVNDCIKFIPESWMYANISKKYKFVYVPEPVTLFYDDHDHLRLSK
                     SSLKTNAKGQNIARKAVLKNVPVKVWFKNPISALKNSARYFQTLYYLAVEKYENKH"
     gene            3404..4543
                     /gene="wzy"
     CDS             3404..4543
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="QEE84094.1"
                     /translation="MKININKLLLFFMVAICTIDLPAAIIVYGTQIIGLILVAVLILI
                     KVQKISLTAVTIWAGFTIFFVMITYVQSISIEHVSLIWKFAGRVIFWFFIFSLITPFF
                     QELNRRDVVSILQTWIIIFSLFLYAQFLLYYVFGYIVDYSVILGGEPSRILNNVGLRA
                     SGLTSEPSIYSGMMISLLILHYIYNDQKNNFYTLFGGVSILLTFSTLGFFLFTLYLSI
                     TLLRHMSIKKIILLIGTITSVVVLIMPLLISRYERFINGDDVSNNVKFSVVENLLNNT
                     SLFTLGYGVVGYSDGAPDYYQALYDLTLFGNLFVIFGIPIGIFTSVYIFLLLLKIDLP
                     LSIKALILLGVVKISIPNYIFFYLFVLMVVFYKRVLSNENYRFFN"
     CDS             4518..5606
                     /codon_start=1
                     /transl_table=11
                     /gene="orf5"
                     /product="glycosyltransferase"
                     /protein_id="QEE84095.1"
                     /translation="MKTIVFLIDGLLNSGGTERLTVVTANNLALSGYNIHIITLRESS
                     KYFFKVNHTVNVSSLAFCKKRNYFLTKILYIKEIRRYLKEISADCIVAVDSLLSVYSI
                     PASMGLKTYHITWEQFNFDINLGMRLRDVSRFLSSFFSSKIIVLTNKDKLKWLKKYHW
                     SSNKIHVIFNPSFINVTDKKYSDDSKIILAVGRLAYQKGFDLLIKNWAEICNDYQGWK
                     LRIIGSGNDELYLKELAKTLDVDEKVEFAGQISNISDEYNKASFFCMSSRFEGIGLVL
                     IEAQACGLPLIAYDCDCGPSEVIKNSENGYLIPAFNDAAYKNAMRKMIENPSLRQQFS
                     KECKHNADVFSTESFCQAWIQLIQKISE"
     CDS             5611..6672
                     /codon_start=1
                     /transl_table=11
                     /gene="orf6"
                     /product="glycosyltransferase"
                     /protein_id="QEE84096.1"
                     /translation="MKVVHVIPRLKLGGVEAAVLSSYDYLISKGINFKILALEKGTNK
                     YNNSNIFSLNSSVISILLYLRFFKFVCREKPDLMIFSLWKTSFLGILLVLFRKLIRVQ
                     TRTAIIIHSSKHAHIFDKYVTNLAVKLLDNVYFDSQEAKALYNIEGEIISFILRKPEC
                     IPTKVYDLNYSFVFIGRVHPVKNILAALNFIRLIKSQNIKVIFDIYGPDEGGLEEVQN
                     YIRNYNLYDVVSIKGPVEGAAIKNILPNYTFYLQFSKYEGMAISVVEAMQYGLIPCVT
                     NVGEINNYCKDDYNAFIFDLSKINSDCYLVDKLFKIRTALENNNEYEYISNNARKTFL
                     HSRTYKEDVMSKVIHNKKN"
     CDS             6681..7805
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="glycosyltransferase"
                     /protein_id="QEE84097.1"
                     /translation="MKAVALSANTCWYLFNFRTNTIKKLISNGYMVYIISGKDDYVHK
                     LEGLGCTFIELAIKPKSKNPFVDIRTIFHYIKIFREIKVDAVLNFTPKCNIYSTIAAS
                     LCNIPSLNNISGLGAVFSSQGIVRIITEALYKFSQKRARVVFFQNKDDMKLFTQLVDF
                     SNDTDVMLLPGSGVDLKRFKQLPYVDKKEGVVFLMSSRLLEEKGVTIYYEAAKRIRNI
                     YPSVNFYLAGFVEKECANAISADDIERWQNEGAIKYLGSTDKIEEFLSMADCFVLPSY
                     YSEGVPKSLLEAAAMGLPIITTDNIGCREVIVHNESGYLCPPRSVKGLFDAMVKFIEL
                     KQEDRILMGKRARIKIEQEFNEEVVIEKYISALTNILHKQ"
     gene            7892..9298
                     /gene="gnd"
     CDS             7892..9298
                     /gene="gnd"
                     /codon_start=1
                     /transl_table=11
                     /product="Gnd"
                     /protein_id="QEE84099.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            9520..10713
                     /gene="ugd"
     CDS             9520..10713
                     /gene="ugd"
                     /codon_start=1
                     /transl_table=11
                     /product="Ugd"
                     /protein_id="QEE84100.1"
                     /translation="MFNRKKSELMKITISGTGYVGLSNGILIAQNHEVVALDIVQAKV
                     DMLNKKQSPIVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFN
                     TSSVEAVIRDVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYD
                     NLHPSRIVIGEQSERAERFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVA
                     YFNELDSYAESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQS
                     VPNNLISAIVDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRI
                     KAKGVPVIVYEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRD
                     LFGND"
     gene            complement(10779..11783)
                     /gene="wbnF"
                     /note="gla"
     CDS             complement(10779..11783)
                     /gene="wbnF"
                     /codon_start=1
                     /transl_table=11
                     /product="WbnF"
                     /protein_id="QEE84101.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFAEERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPQWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            12152..13189
                     /gene="wzz"
     CDS             12152..13189
                     /gene="wzz"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz"
                     /protein_id="QEE84102.1"
                     /translation="MMIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETA
                     EGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQ
                     TRQNLLDIESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVL
                     GRNALRNYNAK"
     gene            complement(13287..13898)
                     /gene="hisI"
     CDS             complement(13287..13898)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="HisI"
                     /protein_id="QEE84103.1"
                     /translation="MLTEQQRRELDWEKTDGLIPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4178 a   2460 c   2952 g   4365 t
ORIGIN
        1 ctggaaaaat ggagccatac ttaggaacaa tgctactgca atctacaacg aagcggcgta
       61 acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa aaaatcgaac
      121 aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt taaaagcagt
      181 tattcctgta gcgggtcttg ggatgcatat gttgcctgcc actaaggcga ttcccaaaga
      241 gatgctaccg atcgtcgaca agccaatgat tcagtacatt gttgatgaga ttgtggctgc
      301 agggatcaaa gaaatcctcc tggtaactca tgcgtccaag aacgcagtcg aaaaccactt
      361 cgacacctct tatgaattag aatctctcct tgagcagcgc gtgaagcgtc aactgcttgc
      421 ggaagtacag tccatttgcc cgccgggcgt gacaattatg aacgtgcgtc agggcgaacc
      481 tttaggtttg ggccactcca ttttatgtgc acgacctgcc attggtgaca atccatttgt
      541 cgtggtgctg ccagacgttg tgatcgacga cgccagcgcc gacccgctgc gctacaacct
      601 tgctgccatg attgcgcgct tcaacgaaac gggccgcagc caggtgctgg caaaacgtat
      661 gccgggtgac ctctctgaat actctgtcat ccagaccaaa gagccgctgg accgtgaagg
      721 taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga cgctggactc
      781 agacatcatg gccgttggtc gctatgtgct ttctgctgat atttggccgg aacttgaacg
      841 cactcagcca ggtgcatggg gacgtattca gctgactgat gccattgccg aactggcgaa
      901 aaaacagtcc gttgatgcca tgctgatgac tggtgacagt tacgactgcg gcaaaaaaat
      961 gggctatatg caggcgtttg tgaagtatgg actacgcaac ctgaaagaag gggcgaagtt
     1021 ccgtaaaggg attgagaagc tgttaagcga ataatgaaaa tctgaccgaa agtaacggtt
     1081 gataagaaaa ttataacggc agtgaagatt cgtggcgaaa gtaatatgct gcgaatattc
     1141 ctgccgttgt tttatataaa caatcagaat aacaaagagt tagcaatagg attttcgtca
     1201 aagttttcca ggattttcct tgtttccaga gcggattggt aagacaatta gcgtttgaat
     1261 ttttcgggtt tagctagtcg taagttgttt agcaaacttt ctgatgggta attttcaggg
     1321 gtctggtaac tgttgagcta ggggcggtag catgcatgta ttccaataat ttaaaaaagt
     1381 tagttactca tcctgtatta ctaaatatat actggttgat gtctgaaaaa attttgactg
     1441 tgggtttgat attttatgca gaaggtttaa tagctaaatt attaactaaa tatcagtatg
     1501 gtgaatggat atacagttta aacttagtca cgcttatatc gtcccttgct cttatcgctg
     1561 gtgctgaagt tgcagtacca gcattatcaa ggaataaatc tttaacagat gaaataataa
     1621 cgtccctctt taagattcgg ttcttttttg cactgatagc ttttttggca ttaaatgtat
     1681 attgcgttat atttgttgag ccgtctttgt taaaaaaatt cataatgatg ttatctgtca
     1741 ttattatatt gaatgaaccc ttttcagttg taataaatta ttaccaatca ataacaaaga
     1801 ttaaatcaat agcattgctg cgattatttt cattagcttg tcgaaccttt tttatatggt
     1861 tatgttattg tttgactctt caggcaaatt tacttgctct tagtcgtgtt attgaattgt
     1921 ttgttttagc gatatcatta atatttcttt ggaaatataa gaagtttcga tggaaagtta
     1981 aacctggtat tactaagttt ttatttatac gaggcgcttc gttatggccc tcattggttt
     2041 taatgtattt atacttaaga atggaccgtt tttttgttga acactactta tcatttgagg
     2101 ttttggctgt ctatggtata gctgtacaat taactgagca aacatttgtt ttagttaaaa
     2161 tgattataca gtcggcagca ccgagatacg tatatgccag aatgaatcat gctaatttat
     2221 taaggaatat taaaaaatta attttattta atgtaataat atgctcttca atcagtgtgt
     2281 tatgtttttt tgttttgcca cttattgtaa caagtttatt tggtgagaat tatcagagag
     2341 cagcaagttt agctattaat atgcttcctg cgctgttctt ttattcaatt gatagtgtga
     2401 taatgcagta tttatacaga gaaaagaaaa gtatccttat attgtttaaa tggggggcgg
     2461 ggactatatt aatgtttgtt tcctattata tgtattttga ttttttcgaa atgaaagtat
     2521 tatcgataat atataatcta aactatttct gcatgatgct attgacaata acagtttttt
     2581 tatgggggag gcagtatgat aagtaaaaaa atagttattt ttacaccgac ttataacaga
     2641 aaacacacat tagaaagatg ttatcagagt attattcagc aagattatcc aggtgttgta
     2701 tggcttgtga ttgacgatgg ttcgacagat aacacagagc aattagttac tgagtttatt
     2761 aaagaaggca agttagaaat acaatatata taccaaacaa atggaggaaa gcaatctgct
     2821 tggaataaag ctgtccaatt atgtcagaag tatgatcttt ttatatgtgt tgattctgat
     2881 gatattttat atgagaacgc tctaactaag gtgtcaaagt atttctcttt agttgatgaa
     2941 gacgatgtaa taggactgcg ttgcttagct atcagaaata caaccaaaaa agctgatagt
     3001 aagtttatga ataataagat acataaagac tactggttta tggagataat gagtaatcag
     3061 atcggtgaac gagtggatat atttaaaccg cagaaattac ttgattattt atttcctgtg
     3121 aacgattgca tcaaatttat acctgaaagt tggatgtatg ctaatatatc gaaaaaatat
     3181 aaatttgttt atgttcctga acctgtgaca ttattttatg atgaccatga tcatctgcga
     3241 ttaagtaaat cctcattaaa aactaatgct aaaggtcaaa atattgcgcg taaagcagta
     3301 ttaaaaaatg tgccagttaa agtttggttt aaaaatccaa tttcagcatt aaagaattcc
     3361 gcacgttatt tccaaacact ttattatctc gctgtggaaa aatatgaaaa taaacattaa
     3421 taaattacta ttgtttttta tggttgcaat atgcacaata gatttgcctg ctgcaattat
     3481 tgtctatggc actcaaatta ttggacttat attagttgct gtcctgatat tgattaaagt
     3541 gcaaaaaatt tcattaacag ctgttaccat ttgggcagga tttactatat tttttgtaat
     3601 gattacatat gttcaaagta taagtattga acatgtaagt cttatatgga aatttgctgg
     3661 tcgggtaata ttttggtttt ttatattcag tttaataaca ccatttttcc aagagttgaa
     3721 tagaagagat gtggtatcaa ttctacaaac atggataata atattttcat tatttcttta
     3781 tgctcaattt ctcttatatt atgtttttgg ctatattgtc gattattctg ttatattagg
     3841 aggagagcct tcgcggatat tgaataatgt agggctacgg gcatcaggtc taacatctga
     3901 gccctctatt tattcaggga tgatgatatc gttactgata cttcattata tatataatga
     3961 tcaaaaaaat aatttttaca cgttgtttgg aggggtaagc attctgttga ctttttcaac
     4021 gcttggtttt tttcttttta ctttatattt atctattact ttgctaaggc atatgagtat
     4081 caaaaaaatt atccttttaa taggaactat aacaagtgtg gtagtgctta ttatgccatt
     4141 actgatctca cgatatgaac gttttataaa tggtgatgat gttagtaata atgtaaagtt
     4201 ttcggttgtt gaaaatttgt tgaataacac ttcacttttt actttggggt atggagtagt
     4261 tggttatagt gatggcgcgc cagattacta tcaggctcta tatgacctta cgttatttgg
     4321 aaatctattt gtcatatttg gtattcctat tggcatcttt acttctgtat acatattctt
     4381 gttgctttta aaaatagacc tgccattaag tataaaagca ttgatactat taggagttgt
     4441 taagatttca atcccaaatt atatattttt ctatttgttt gttttaatgg ttgtttttta
     4501 taaaagagtg ttatctaatg aaaactatcg tttttttaat tgatggatta ttaaactctg
     4561 gtggcactga gagattaact gtcgtaacag ccaataattt agctttatct ggttataata
     4621 ttcatattat tacacttcgt gaaagttcaa aatatttttt caaagttaat catacagtta
     4681 atgtttcaag tttggctttt tgtaagaaaa gaaattattt ccttactaag atactatata
     4741 ttaaggaaat tcgaagatat ttaaaagaaa tatcggcaga ttgtattgtc gcagttgatt
     4801 ctttgctctc tgtatattca attcctgcat caatgggcct taaaacatat cacataacat
     4861 gggagcaatt taattttgat attaatttag gcatgagatt aagagacgtt tctcgttttt
     4921 tatcatcttt tttttcaagt aaaattattg ttcttacaaa taaagataaa ttaaaatggt
     4981 taaaaaaata tcattggtct agtaataaga ttcatgtaat ttttaatcct tcatttatta
     5041 acgtgacaga taaaaaatac tccgatgata gtaaaattat actggccgtg ggaagattag
     5101 catatcaaaa aggatttgat cttttaataa aaaattgggc ggagatttgc aacgattatc
     5161 aagggtggaa actaaggatc attggttcag gtaatgatga attgtattta aaagaattgg
     5221 ctaagacctt agatgtagat gaaaaagtag aatttgcagg gcaaataagt aatatttctg
     5281 atgaatacaa taaagcatca tttttctgca tgtcatcaag atttgaagga ataggattgg
     5341 ttttaattga agcgcaggca tgtggattac cacttatagc ttatgattgt gattgtgggc
     5401 cgtcagaggt aattaaaaat tcagaaaatg gttatttgat cccggcattt aatgatgcag
     5461 catataagaa tgccatgagg aaaatgattg aaaatccatc attgagacaa cagttttcta
     5521 aagaatgtaa gcataatgca gatgtttttt ctacagaaag tttttgtcaa gcatggattc
     5581 aattgattca gaaaataagt gagtaatttt atgaaggtag tacacgttat tccaaggctt
     5641 aaactcggag gggtagaggc agctgttctt agttcgtatg attatttaat aagtaaaggt
     5701 attaacttta aaatactggc tcttgaaaag ggtaccaaca aatataataa ttccaatatt
     5761 ttttcactta attcgtcagt gatatctata ctattatatt tgaggttttt taagtttgta
     5821 tgccgcgaaa aaccagatct aatgattttt tcactttgga aaacgtcttt tcttggtatt
     5881 ttacttgtat tgtttcgtaa attaattaga gttcaaacac ggacagcaat aataatacat
     5941 agctcaaaac atgctcatat atttgacaaa tatgtgacaa atcttgccgt aaaactatta
     6001 gataatgtct attttgactc acaggaagca aaagcattat ataatattga aggtgaaatc
     6061 ataagtttta ttttacgtaa acctgagtgt attccaacca aggtatatga tttaaactat
     6121 tcttttgttt ttataggtcg ggttcatccg gtaaaaaata tattggcagc gcttaatttt
     6181 attcgtctaa taaaatctca gaatattaaa gttattttcg atatctacgg gcctgatgaa
     6241 ggaggattag aggaagtaca aaattatatt aggaattata atctttatga tgtcgtatct
     6301 ataaagggac ctgtcgaagg ggctgcaatt aagaatattt tacctaacta tactttctat
     6361 ttacagttta gtaagtatga gggtatggca atatctgttg tcgaagcgat gcaatatggt
     6421 ttgattccgt gtgtaacaaa cgttggagaa attaataatt attgcaagga tgattataac
     6481 gcttttatat ttgatttgag taaaattaat agcgattgtt atttagttga taagctattc
     6541 aaaattagaa cggcattaga aaacaacaat gaatatgaat atatttctaa taatgctcgg
     6601 aaaacatttt tgcattcccg gacatataaa gaggatgtca tgagcaaagt aattcacaat
     6661 aaaaagaact aaggtaaaat atgaaagcag tagctttgag tgcaaatact tgctggtatt
     6721 tatttaattt ccgaactaac accattaaaa aactaatcag caatggttat atggtatata
     6781 ttatttccgg taaagatgat tatgttcata aattggaagg gctggggtgt acttttatag
     6841 agcttgcaat aaagccaaag agtaaaaatc cttttgttga tataaggaca atatttcact
     6901 atattaaaat tttcagagaa attaaagttg atgctgtgtt gaatttcact cctaagtgta
     6961 atatttattc tactatagct gcatcgttat gcaatattcc atcattgaat aatatttcag
     7021 gacttggtgc tgtattttca tcgcaaggga tcgtgcgaat tattacagaa gcgctttaca
     7081 aatttagtca aaaaagagca agggtggtat tcttccaaaa taaggatgat atgaagcttt
     7141 ttacacaact tgtggacttt agtaatgata cagatgtcat gttattacct ggttcagggg
     7201 tagatttaaa gcgatttaaa cagttgccct atgttgataa aaaagaggga gttgtttttt
     7261 taatgtcttc aagattattg gaagaaaaag gagttactat ttactacgag gctgcaaaaa
     7321 ggattagaaa tatataccct tctgttaact tctatttggc tgggtttgtg gagaaagaat
     7381 gtgctaatgc aatttctgct gatgatatag aacgttggca aaatgaaggc gcaataaaat
     7441 atcttgggag tacagataaa attgaagaat ttttatcaat ggctgattgt tttgtcttgc
     7501 catcatatta tagtgagggg gtgccaaaat cattactcga agcagcagcg atggggttgc
     7561 caatcattac aacggataat ataggttgta gggaagtaat tgttcataat gaatctggat
     7621 atttatgccc tccacgttct gttaaaggtc tttttgatgc aatggttaaa tttattgaac
     7681 ttaagcaaga agatagaatt ttgatgggta aacgagccag aataaagata gaacaagagt
     7741 tcaacgaaga agttgtaatt gaaaaataca tcagcgcatt aactaatata cttcataaac
     7801 aataattaat tggtaatatc tgtaaaactt ttttgagtta taattgttta tcattatact
     7861 tcatagtttt gcaaactgac aggagtaaac aatgtcaaag caacagatcg gtgtcgtcgg
     7921 tatggcagtg atggggcgca acctggcgct caacatcgaa agccgtggtt ataccgtctc
     7981 tattttcaac cgctcccgtg agaagacgga agaagtgatt gccgaaaatc caggcaagaa
     8041 actggttcct tactatacgg tgaaagagtt tgttgaatct ctggaaacgc ctcgtcgcat
     8101 cctgttaatg gtgaaagcag gtgcaggcac ggatgctgct attgattccc tcaaaccata
     8161 tctcgataaa ggcgacatca tcattgatgg tggtaacacc ttcttccagg acaccattcg
     8221 tcgtaaccgt gagctttctg ctgaaggctt taacttcatt ggtaccggtg tctccggtgg
     8281 agaagaaggc gcgctgaaag gtccttccat tatgcctggt gggcagaaag aagcctatga
     8341 actggttgcg ccgatcctga ccaaaatcgc cgcagtggct gaagacggtg agccatgcgt
     8401 tacctatatt ggtgccgatg gcgcaggtca ctatgtgaag atggttcaca acggtatcga
     8461 atacggtgat atgcaactga ttgctgaagc ctattctctg cttaaaggtg gcctgaacct
     8521 caccaacgaa gaactggcgc agacctttac cgaatggaat aacggtgaac tgagcagcta
     8581 cctgatcgac atcaccaaag atatcttcac caaaaaagat gaagacggta actacctggt
     8641 tgatgtgatt ctggatgaag cagcaaacaa aggtacgggt aaatggacca gccagagtgc
     8701 gctggatctc ggtgaaccac tgtcgctgat taccgagtct gtgtttgcac gttatatctc
     8761 ttctctaaaa gatcagcgcg ttgccgcatc taaagttctc tctggtccgc aagcgcagcc
     8821 agctggcgac aaggctgagt tcatcgaaaa agttcgtcgt gcgctgtatc tgggcaaaat
     8881 cgtttcttac gcccaaggct tctctcagct gcgtgctgcg tctgaagagt acaactggga
     8941 tctgaactac ggcgaaatcg cgaagatttt ccgtgctggc tgcatcatcc gtgcgcagtt
     9001 cctgcagaaa atcactgatg catatgccga aaatccgcag atcgctaacc tgctgctggc
     9061 tccgtacttt aaacaaattg ccgatgatta ccagcaggcg ctgcgtgatg tcgttgctta
     9121 tgcagtacag aacggtatcc cggttccgac cttcgccgct gcggttgcct attatgacag
     9181 ctaccgtgcc gctgttctgc ctgcgaacct gatccaggca cagcgtgact atttcggtgc
     9241 gcatacttat aagcgcattg ataaggaagg tgtgttccat actgaatggc tggattaatc
     9301 tgatttaaat caattaacca acacaaggcc cggagaaacc ctccgggctt ttttattata
     9361 caaagcggca ggttatggcc tttttttata atttatgatt aaaaaagcga tataatacag
     9421 agccgcacag caggatcgct gccttgacag ttcatataca tcagcgttaa aaatcccgca
     9481 gtagatgaag ttgtggtggt ggattaatga ccactctaaa tgtttaaccg gaagaagtca
     9541 gagctaatga aaataacaat ttcaggaaca ggttatgttg gtctttcaaa tggtattctg
     9601 attgcgcaaa accacgaagt ggttgcactg gatatcgttc aggccaaagt ggacatgctt
     9661 aacaagaagc agtcaccgat tgttgataag gagattgaag agtatctggc gactaaagat
     9721 ctcaatttcc gcgctacgac agataagtat gacgcgtata aaaatgccga ttacgttatt
     9781 attgccacac ctaccgatta tgatccgaaa acgaattatt ttaatacctc aagcgtggaa
     9841 gcggtcattc gcgatgtgac agaaattaat cccaacgcgg taatgattat aaaatcaact
     9901 atccctgttg gttttacaga gtccattaaa gaacgttttg gtattgaaaa tgtgatcttt
     9961 tcgcctgagt ttttgcgtga aggtaaagca ctttatgata acttacaccc atcacgcatt
    10021 gtgattggcg agcagtctga acgcgctgaa cgttttgctg cgttattaca ggaaggcgcc
    10081 attaagcaag acataccaac attgtttact gactcaaccg aagctgaggc gattaaactt
    10141 tttgccaaca cttatctggc tatgcgtgta gcgtatttca atgaacttga tagttatgct
    10201 gaaagcctgg ggcttaattc acgccagatt attgagggcg tatgccttga tccgcgtatc
    10261 ggtaatcact acaacaaccc gtcattcggt tatggtggtt attgtctgcc gaaagatacc
    10321 aagcagttac tggcaaatta ccagtctgtg ccgaataacc tgatctcggc aattgttgac
    10381 gccaaccgca cgcgcaaaga ttttattgcc gattctatcc ttgcacgtaa accgaaagtt
    10441 gttggcgtct atcgtctgat tatgaagagt ggttcggaca atttccgtgc ttcctcgatt
    10501 cagggtatta tgaagcgaat caaggcgaaa ggtgtgccag taatcgttta tgagccagcg
    10561 atgaaagagg acgatttttt ccgttcgcgc gtggtacgtg atctggatgc gttcaaacaa
    10621 gaagctgatg ttattatttc taaccgtatg tctgccgatc tggctgatgt agcagataag
    10681 gtttatacgc gcgacttgtt tggcaatgat taattatttt gtttcatact aagaaaaggc
    10741 cctaataaac tagggccttt tcttatggtt ttataaaatc aaactttata gaagttacga
    10801 taccattcta caaagttctt taccccctct ttaactgacg tttcaggttt gaatcctatt
    10861 acgtcataca gtgcttttgt atcagcactg gtttccagta catcaccggg ttggagaggc
    10921 atcatatttt tgttggcttc aatacccaga gcctcttcta acgcattgat atagtccatc
    10981 aactccacag gcgaactatt accaatgtta tagacacgat atggtgctga acttgttgca
    11041 ggcgagcctg tttcaacagc ccactgcgga tctttttctg gaataacatc ctgtaagcga
    11101 ataatagctt cggcaatatc atcaatataa gtaaagtcac gcttcatttt gccgaagttg
    11161 taaacatcaa tgcttttacc ttccagcatg gctttagtga atttaaataa tgccatatcc
    11221 ggacgtcccc atggaccata aaccgtgaag aaacgcagcc ctgtggtcgg taagccatac
    11281 aaatgagaat acgtatgggc catgagttca ttcgcttttt tagttgctgc atacagcgaa
    11341 acaggatgat ctacagagtc atctgtagag aaaggcatct tgcggttcat gccataaaca
    11401 gaactggagg aagcgtaaag tagatgctga acattattat ggcggcatcc ttccagtatg
    11461 ttcaggaatc caatcaggtt tgcatctgca tatgcattgg gattttcaag ggagtaacgt
    11521 acaccggctt gcgcagcgag gtttattacg cgatcgaacc gctcctctgc aaacagtgcc
    11581 gccattttct cacgatcggc caggtcaatt ttataaaaac tgaagttgtc gtgcttgagt
    11641 aaatcaagtc gtgcttgttt gaggttgaca tcgtaataat catttaagtt gtcaatgcct
    11701 acaacctgat gaccagctgc aagaagccgt ttacttacat agaaaccgat aaagccagca
    11761 gctcccgtaa ccagaaattt catttataat cctcgctcag gctagaatat agccaatctt
    11821 catctggcat aactgaaagt taaatcatac cgttagacaa gaaaaaaaga taatcggtat
    11881 cagttctaaa cctggctgtt ttttctggta acgtgctcat tttgcaatca aacctgttct
    11941 aagctgacta ttagaccacc ttaaaacaac aagccgatgt cattatttcc aaccgtatgg
    12001 cagaagagct taaggatgtg gcagacaaag tctacacccg cgatctcttt ggcagtgact
    12061 aacatcctgt tatcagggcg attttcgccc tgattctctt atgttccctt tgtaataatt
    12121 cattattttt atcatttatc ctatagcatt catgatgatt atcgctaaac tatggcgact
    12181 taaaattatt tcgtccgtta gggtaatgat gagagtagaa aataataatg tttctgggca
    12241 aaaccatgac ccggaacaga ttgatttgat tgatttactg gtgcagttat ggcgtggcaa
    12301 gatgacaatt atcatctctg tcattgtggc tatagctctg gctatcgggt atctggccgt
    12361 tgccaaagaa aagtggactt ccacagctat cgttactcaa cctgatgtgg ggcaaatcgc
    12421 tggttatacc aacgcgatga atgtgatcta cggatcggct gtaccgaaag tctccgacat
    12481 tcaggcttca cttatcggac gttacagcac tgcattttca gcattagcgg aaacgctgga
    12541 taaccaggaa gaagcagaga agctgaccat tgagcctacc gttaaaaacc aatcgttacc
    12601 attggcggtt tcttatgtgg gtgaaacagc tgaaggtgcg cagaaacagc tggcgaagta
    12661 tatccagcaa gttgacgacc aggtaaacga agaactggaa aaagacctga aagacaacat
    12721 cgcgctgcgt atgaaaaact tgcaggattc gctgaagacc caggaagtgg tcgcgcagga
    12781 gcagaaagat ctgcgtatcc gtcagattca ggaagcgttg cagtatgcga atcaggcgca
    12841 ggtgacaaaa ccgcagattc aacagactgg cgaagatatc acacaagata cgttgttcct
    12901 tttggggagc gaagcgctgg agtcgatgat taagcacgag gcgacccgtc cgttggtgtt
    12961 ctcaccaaac tactatcaga ctcgtcaaaa cctgctggat atcgaaagct taaaggttga
    13021 tgatcttgat attcatgctt accgttatgt gatgaaaccg acgttaccta ttcgtcgcga
    13081 tagtccgaaa aaggcaatca ccttgattct ggcagtgctg ctgggcggca tggttggcgc
    13141 ggggattgtg ttggggcgta acgctctgcg taattacaac gcgaagtaat cttttcggtt
    13201 ttaaagaaaa tgggcagggt ggtgacacct tgcccgtttt tttttgccgg atgcgacaac
    13261 aatatcgcat ccgcttaccc cgcaactcac tgatgccgtt tacgcaggtt ctcaattacc
    13321 gtagttaaat ccagatcctg atcctgcaac agcaccagca ggtgatacat caaatcagat
    13381 gcctcgttgg tcagctcaaa gcggtcatgt accgttgctg ccagcgcggt ttccacgcct
    13441 tcttcaccca ctttctgcgc aatgcgtttg gtgccgctgg catacagttt ggcggtgtag
    13501 gaggtttccg gatcggcaga tttgcgctcg gcgagcagtt gttctagttg atacaggaac
    13561 agccactggt gagcggtgtc gccgaagcaa ctgctggtgc ctttgtggca ggtcgggccg
    13621 atgggattcg ccagcaccag taacgtgtcg ttgtcgcagt ccggggcaat actcactacg
    13681 ttgaggaaat tgcccgacgt ttcgcctttg gtccacagtc gctgtttagt gcgcgagaag
    13741 aaggtgactt tgccgctttc gagggttttg tctaaggctt ccgggttcat atagcccagc
    13801 attagcactt cgccggatac cgcgtgttgc acaatcaccg gtataagtcc gtcggttttt
    13861 tcccagtcca gttcgcgacg ttgttgttct gttaacatat cctgatctcc acgccctgtg
    13921 ttgccaggta cgcttttaat tcaccaatat tgatt
//
