LOCUS       MN172355               22100 bp    DNA     linear   BCT 24-AUG-2019
DEFINITION  Escherichia coli strain 16-02258 O-antigen biosynthesis gene
            cluster, complete sequence.
ACCESSION   MN172355
VERSION     MN172355.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22100)
  AUTHORS   Lang,C., Hiller,M., Konrad,R., Fruth,A. and Flieger,A.
  TITLE     Whole genome-based public health surveillance of less common STEC
            serovars and untypable strains identifies four novel O genotypes
  JOURNAL   J. Clin. Microbiol. (2019) In press
   PUBMED   31366691
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22100)
  AUTHORS   Lang,C., Hiller,M., Konrad,R., Fruth,A. and Flieger,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2019) Division of Enteropathogenic Bacteria and
            Legionella (FG11), Robert Koch Institute, Burgstrasse 37,
            Wernigerode, Saxony-Anhalt 38855, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: A5 v. 2.1.3
            Assembly Name         :: OgN-RKI
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="16-02258"
                     /isolation_source="stool"
                     /db_xref="taxon:562"
                     /country="Germany"
     misc_feature    1..22100
                     /note="O-antigen biosynthesis gene cluster"
     gene            82..975
                     /gene="galF"
     CDS             82..975
                     /gene="galF"
                     /codon_start=1
                     /transl_table=11
                     /product="GalF"
                     /protein_id="QEE84071.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            1347..2432
                     /gene="rmlB"
     CDS             1347..2432
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="QEE84072.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPHAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KKLPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRK"
     gene            2432..3331
                     /gene="rmlD"
     CDS             2432..3331
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="QEE84073.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDMPWLETDATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIALALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3389..4264
                     /gene="rmlA"
     CDS             3389..4264
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="QEE84074.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEIAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKG"
     CDS             4272..4823
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlC"
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="QEE84075.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQNIFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSTTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTQYYHPECERGIRWDDQTINIKWGNI
                     ASHIILSEKDEKACSFIDAVCFS"
     gene            4834..6243
                     /gene="wzx"
     CDS             4834..6243
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="QEE84076.1"
                     /translation="MMSFKVYKWSFIERILVQLFQLIIIITLSRFLTPDDFGLFGLSA
                     VTISIAQVFVDGGFSAALIRKKTHSDVELNSVFIINIFIALLLISLVLVCSPLIENYF
                     NIDGLANLLNLSSLVILINAFSVIPKVVLTVNLNFRGIAESSLIAVLSSGILSIILAV
                     NNFGASTLVFQLMIYNLINLILIIAKSKWLPRKCFDMQSVKELCNFSIKLFASGLLDA
                     LYQNIYLVLIGKLMSVHDVGIFAQAKKLSDIPALTITTALQRANLAATSKLDENDDIK
                     KSVLKTLQLGMFIMAPAMFFIISSSEPLVNVLLGDNWKSVSGVITILSLSGLCYPIHV
                     LNQNILIIKGRSDLHLKLEIVKKCVGLVLIFITISHGLTWLCWGIVISSILSIFVNAY
                     YSNNLVHVTICEQLKVCITPIFLAIISLIISTLLCSIPINSCTLIFLNIKDVLLSAVT
                     FMIVFVVAISYFCRKENFF"
     CDS             6268..6990
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="glycosyltransferase"
                     /protein_id="QEE84077.1"
                     /translation="MILSIIIPSYNSAHLLLNTLESIRKQKCKRTDVEVIVVDAVSTD
                     HTVDIVNKYGDIISDFICEKDEGIYDAMNKGIDVARGKFSYFIGAGDTLTDNILAEIL
                     PILTKYDGIVYGNVIRTKNNAIYDGEFSIIKLFRRNICHQAIFYHTDIIKALKYNTKY
                     KVLADYHLNIILFSKFNIAKRYINFNIAYYDDLAQGFSQNNSDDAFISDYYRIICENY
                     SLKYKLVLFCISIIRKIKKVIG"
     gene            6987..8309
                     /gene="wzy"
     CDS             6987..8309
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="QEE84078.1"
                     /translation="MSNQEYSQYLFFPNILLFFTIFLLLFLFFIAWFKNNLYSWLDPL
                     LLAILFSSAGVSTVIFLYVLGEIKVNYLLQTMLSQLMLMLGYKILSRRETSYLNKQVK
                     HSTYEQLNTGYPRAFLASSFLFIVAQASTYFLLGIPLLMANRLMIYQEGGFGVLGRII
                     DATFVISFYTFLDMKVRFKVRGLFKAYGYIYLLFLIISLVLNGSKAGILNLVFIIGIF
                     CYLNKLKFNISLISIYSKFARRLLIFAFISMFIILMVNYGIFSDQNYNRIIDLLGVIF
                     LRIFNSGDIYVLSYPNDALFKIPAEHNGIISLFKDLLATFRIISTNEIPPSLGNIAFN
                     IYSTTEIGGANARQSLFGYYYFGFMGSLLFSFFLGLTMSFFRHVLPRIKKATPLAMGV
                     LCYFILNFSFLDIDPSYQFSKLTNFFIVTPLIILLMGVMPNIRRQKNV"
     CDS             8302..9180
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="glycosyltransferase"
                     /protein_id="QEE84079.1"
                     /translation="MFSIIIINWNSYTETMNCIASILANRELITRIIIVDNNSSDDSL
                     KKLKQEFIYNDLVKIIESKTNTGYCGGNNLGFQYCLNDNSNYLLVLNNDVIITRRTLS
                     LLKKAFEKDEKLVMVSPMVNGELIENSTFLVRINENLNSTLASTQDDSGFVYKYYIPG
                     CAIAFSKNFLKNYGGFNENYFMYVEEIEIAFRARKNGYNVAQLTSFDSSILRKKDDIE
                     KLRKRYIWYYQTRNMLYFLDDIYMRNYWKGVFKKSLYITFSFIRPFTQRNLGNLIAVY
                     KGVGDYLKGVKGKQVV"
     CDS             9196..10359
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="glycosyltransferase"
                     /protein_id="QEE84080.1"
                     /translation="MNILIVMPHFYPAANYGGPVISSFKTCKELAKLGHNVSVSTSNV
                     NQNGKLDVKLNTRIKISDNFYVKYYNETIKNRFSLPLTLGVFRDIKNSDVVHIQYVFN
                     YSTVVALLYSYILRKKTILSPRGCLGKWCLEHGSRLKIFWNKLLIKPLTKNTIWHATS
                     KQECDDIINQFGDVKVIISPNGIDLEEYKDITPISKKEFISRYIPNYSRNDYNKLVVS
                     LGRIQKKKGFDILIKSFTNLLNDFPESILVIAGPDEGEKENLFDLIAKNKMQDNIFII
                     DSIYGRDKLHFLNAADVFALPSHNENFGNVYLESLAAGTPILASKHTPWSIVEQFGCG
                     QWVNNDEVSVTNALKLLFEGDAYHLHKNSLKCAQNYSWKNSAEIISNALDNLK"
     CDS             10367..10897
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="QEE84081.1"
                     /translation="MILSEYKKIYHAPKVKSIIWYVLNFIFFETAIPYPSQIKCFILK
                     LMGAHIGKGVVIKPNVKIKYPWNLYIGNNSWLGEKVWIDNLDIVSVGNNCCISQGAYL
                     LTGNHDYRSEYFDLIISPITIESEVWVGAKAIVTPGVHLKTGAILAVNSVAVSDLNEN
                     SVYQGNPATFKRKRYS"
     gene            10897..12015
                     /gene="gmd"
     CDS             10897..12015
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmd"
                     /protein_id="QEE84082.1"
                     /translation="MKKALITGVTGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERVD
                     HIYQDRHNQNPNFFLHYGDLTDTSNLIRLIKEIQPDEVYNLGAQSHVAVSFESPEYTA
                     DVDAMGTLRLLEAIRICGLEKKTRFYQASTSELFGLVQEIPQRETTPFYPRSPYAVAK
                     MYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYLG
                     NMDSLRDWGHAKDYVRMQWMMLQQDHPEDFVIATGKQISVREFVRMSAKEVGLELEFS
                     GQGVDEIATVVNKTSDCAIGVSIGDVIVRVDPRYFRPAEVETLLGDPAKAKKVLGWEP
                     EITVEEMCAEMVASDLAKAKQHALLKSHGYDVAVSLER"
     gene            12019..12984
                     /gene="fcl"
     CDS             12019..12984
                     /gene="fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="Fcl"
                     /protein_id="QEE84083.1"
                     /translation="MTKKRIYVAGHRGMVGSAICRQLSLRDDIELVVKTHKELDLTVQ
                     KDVDAFFEQEKIDQVYLAAAKVGGIYANNTFPAEFIYQNLMIESNIIHSAHKAGIQKL
                     LFLGSSCIYPKFAEQPMNESALLTGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGINDNFHPENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
                     AASVHVMELDEAIYQQNTQPMLSHINVGTGVDCSIREMAETMASVVGYQGKIVFDVTK
                     PDGTPRKLMDVTRLKNLGWQYRYNLHEGLSLTYKWFIENINSFRG"
     gene            12987..13448
                     /gene="gmm"
                     /note="nudD"
     CDS             12987..13448
                     /gene="gmm"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmm"
                     /protein_id="QEE84084.1"
                     /translation="MNKRLERELFKTIVEHTPLISIDLIIRNDKGEALLGQRLNRPAQ
                     NYWFVPGGRIYKDESFEVAFKRITFEELGVKISLNDALFLGVYEHFYNDNFSEAEFST
                     HYVVHGYEIQLNPQQLHLPTVQHNSYKWFDVVTLLNSTIVHQYTKNYFIPR"
     gene            13454..14860
                     /gene="manC"
     CDS             13454..14860
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="QEE84085.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
                     DKIEHKPALVICNESHRFIVAEQLRKEGLKHSGILLEPVGRNTAPAVALAALQAMVTG
                     DDPILLVLAADHEIQNEDNFIDAVLAAKNFAEQGKLVTFGIVPTSPETGYGYIKSGES
                     LDGKGYKVAAFVEKPELHVAQRYISDGGYLWNSGMFMFRASVFIDELKKFRPDILASC
                     QTSLSSSTQDLDFIRLDNSSFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
                     WEISSKDQSGNAIRGDVLVEDATDSYLYSQHRLIGAVGVKDLVVVETKDAVLVAHKDK
                     VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGPRFQVKHVIVLPGHITAKQI
                     HYHRTEHWIVVSGTAKVHLEDKTYLVSENESTYIPVGVPHAIENPGKIPLEIIEVRSG
                     VYLEEDDVIRVPSSGVGY"
     gene            14860..15606
                     /gene="wfgS"
                     /note="wfeY"
     CDS             14860..15606
                     /gene="wfgS"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgS"
                     /protein_id="QEE84086.1"
                     /translation="MRISIITATYNSEKTLLDTLLSLEKQTHPDIEYIVVDGASKDNT
                     IKLIKSNCTKVSKIICEPDKGIYDALNKGIQAASGDVIGFLHSDDLFAYDDAIADIAK
                     TFESTGCDAIYGDLEYVAQNDTTKRIRLWKSGSFSRFKMKVGWMPPHPSFYMKRECYS
                     QFGSFSLDYRISADYDSLLRYILKQRISIAYLPKVLVKMRVGGISNRSVSSMVKKSME
                     DIRIMKQNGIIWPLALAYKNISKLPQFIKK"
     gene            15612..17036
                     /gene="manB"
     CDS             15612..17036
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="QEE84087.1"
                     /translation="MLNVKKIINDSNIAFGTSGARGLVIDFSHDVCAAFTHAFLSVVD
                     DKYNFNKVALAIDNRPSSYEIAQACAYAIKQHGFEVEYHGVIPTPALAHYSMQKNIPC
                     IMVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFSPVGELPHLETSTQGADYY
                     LERYVSLFNPEILKGKRIGVYEHSSAGRDLYAPLFNQMGAEVISLGRSDEFVPIDTEA
                     VSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKA
                     LAIPVSCNTAIEESNKFASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFLLASD
                     LQINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFPSDSSQ
                     QIIKNAISSPNNFFNSLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPELRCYA
                     EASDENQAKQYVTNVLGNITSLIS"
     gene            17233..18639
                     /gene="gnd"
     CDS             17233..18639
                     /gene="gnd"
                     /codon_start=1
                     /transl_table=11
                     /product="Gnd"
                     /protein_id="QEE84088.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYGLVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            18861..20054
                     /gene="ugd"
     CDS             18861..20054
                     /gene="ugd"
                     /codon_start=1
                     /transl_table=11
                     /product="Ugd"
                     /protein_id="QEE84089.1"
                     /translation="MFNRKKSELMKITISGTGYVGLSNGILIAQNHEVVALDIVQAKV
                     DMLNKKQSPIVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFN
                     TSSVEAVIRDVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYD
                     NLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVA
                     YFNELDSYAESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQS
                     VPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRI
                     KAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRD
                     LFGSD"
     gene            20144..21178
                     /gene="wzz"
     CDS             20144..21178
                     /gene="wzz"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz"
                     /protein_id="QEE84090.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIENLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(21275..21886)
                     /gene="hisI"
     CDS             complement(21275..21886)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="HisI"
                     /protein_id="QEE84091.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGNVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     6558 a   3979 c   4802 g   6761 t
ORIGIN
        1 aatcaattca gggtaattga tgctggcgaa aaaaatcgaa caagctataa ttcagcaacc
       61 attttacagg tggatgaaat aatgacgaat ttaaaagcag ttattcctgt agcgggtctt
      121 gggatgcata tgttgcctgc cactaaggcg attcccaaag agatgctacc gatcgtcgac
      181 aagccaatga ttcagtacat tgttgacgag attgtggctg cagggatcaa agaaatcctc
      241 ctggtaactc acgcgtccaa gaacgcggtc gaaaaccact tcgacacctc ttatgaatta
      301 gaatctctcc ttgaacagcg cgtgaagcgt cagctgctgg cggaagtgca gtccatctgt
      361 ccgccgggcg tgaccattat gaacgtgcgt cagggcgaac ctttaggttt gggccactcc
      421 attttatgtg cacgacccgc cattggtgac aatccatttg tcgtggtgct gccagacgtt
      481 gtgatcgacg atgccagcgc cgacccgctg cgctacaacc ttgcagccat gattgcgcgc
      541 ttcaacgaaa cgggccgtag ccaggtgctg gcaaaacgta tgccgggcga tctctctgaa
      601 tactccgtca ttcagaccaa agaaccgctg gatcgcgaag gcaaagtcag ccgcattgtt
      661 gaatttatcg aaaaaccgga tcagccgcag acgctggact cagacatcat ggccgttggt
      721 cgctatgtgc tttctgcaga tatttggccg gaacttgaac gcactcagcc tggtgcatgg
      781 gggcgtattc agctgactga tgccatcgct gaactggcga aaaaacagtc cgttgacgcc
      841 atgctgatga ctggagacag ctacgactgt ggtaaaaaaa tgggctatat gcaagcgttc
      901 gtgaagtatg ggctgcgcaa cctgaaagaa ggggcgaagt tccgtaaagg cattgagaag
      961 ctgttaagcg aataatgaaa atctgaccgg atgtaacggt tgataagaaa attataacgg
     1021 cagtgaagat tcgtggcgaa agtaatttgt tgcgaatatt cctgccgttg ttttatataa
     1081 acaatcagaa taacaacgag ttagcaatag gattttagtc aaagttttcc aggattttcc
     1141 ttgtttccag agcggattgg taagacaatt agcgtttgaa tttttcgggt ttagcgcgag
     1201 tgggtaacgc tcgtcacatc gtaggcatgc atgcagtgct ctggtagctg taaagccagg
     1261 ggcggtagcg tgcatttata cctctattaa tcaaactaag agccgcttat ttcacagcat
     1321 gctctgaagt aatatggaat aaaagagtga agatacttgt tactggtggc gcgggattta
     1381 ttggttctgc tgtagttcgt cacattataa ataatacgca ggatagtgtt gttaatgtcg
     1441 ataaattaac gtacgccgga aacctggaat cacttgctga tgtttctgat tctgaacgct
     1501 atatttttga acatgcggat atttgcgatg ctgctgcaat ggcacggatt tttgctcagc
     1561 atcagccgca tgcagtgatg cacttggctg ctgaaagcca tgttgaccgt tcaattacag
     1621 gtcctgcggc atttattgaa accaatattg ttggtactta tgtccttttg gaagccgctc
     1681 gcaattactg gtctgctctt gatagcgaca agaaaaatag cttccgtttt catcatattt
     1741 ctactgacga agtctatggt gatttgcctc atcctgacga agtaaataat aaagaacaat
     1801 tacccctctt tactgagacg acagcttacg cgccaagcag tccttattct gcatccaaag
     1861 catctagcga tcatttagtc cgcgcgtgga aacgtaccta tggtttaccg accattgtaa
     1921 ctaattgctc taacaattat ggtccttatc atttcccgga aaaattaatt ccattggtta
     1981 ttctcaatgc tctggaaggt aaaaaattac ctatttatgg taaaggggat caaattcgcg
     2041 actggttgta tgttgaagat catgcgcgtg cgttatatac cgtcgtaacc gaaggtaaag
     2101 cgggtgaaac ttataacatt ggtggacaca acgaaaagaa aaacatcgat gtagtgctca
     2161 ctatttgtga tttgttggat gagattgtcc cgaaagagaa atcttaccgc gagcaaatta
     2221 cttatgttgc cgatcgtccg ggacacgatc gccgttatgc gattgatgct gagaagattg
     2281 gtcgcgaatt gggatggaaa ccacaggaaa cgtttgagag cgggattcgg aagacagtgg
     2341 aatggtacct gtccaataca aaatgggttg ataatgttaa aagtggtgcc tatcaatcgt
     2401 ggattgaaca gaactatgag ggccgcaagt aatgaatatt ctccttttcg gcaaaacagg
     2461 gcaggtaggt tgggaactac agcgtgctct ggcacctctg ggtaatttga ttgctcttga
     2521 tgttcactcc actgattatt gtggtgattt tagtaatcct gaaggtgtag ctgaaaccgt
     2581 aagaagcatt cggcctgata ttattgtcaa cgcagccgct cacaccgcag tagacaaagc
     2641 agaatcagaa ccggagtttg cacaattact taacgcaaca agtgtcgaag cgattgcgaa
     2701 agcagcaaat gaagttggag cttgggttat ccattactcg actgattacg tcttccctgg
     2761 aaatggcgac atgccatggc tggagacgga tgcaaccgca ccactaaatg tttacggtga
     2821 aaccaagtta gccggagaaa aagcgttaca ggaatattgc gcgaagcatc ttattttccg
     2881 taccagctgg gtatatgcag gtaaaggaaa taatttcgcc aaaacaatgt tgcgtctggc
     2941 aaaagagcgt gaagaattag ccgttattaa tgatcagttt ggtgcgccaa ctggcgcaga
     3001 gttgctggct gattgtacgg cacatgccat tcgtgtagca ctgaataaac cggaagtcgc
     3061 aggcttgtac catttggtag ccagtggtac cacaacctgg cacgattatg ctgcgctggt
     3121 ttttgaagag gcgcgcaaag caggcattgc ccttgcactc aacaagctca acgcagtacc
     3181 aacaactgcc tatcctacac cagctcgtcg tccacataac tctcgcctta atacagaaaa
     3241 atttcagcag aactttgcgc ttgtcttgcc tgactggcag gttggcgtga aacgaatgct
     3301 taacgaatta tttacgacta cagcaattta atagtttttg catcttgttc gtgatggtgg
     3361 agcaagatga attaaaagga atgatgaaat gaaaacgcgt aaaggtatta ttttagcggg
     3421 tggttctggt acacgtcttt atcctgtgac tatggctgtc agtaaacagc tattacctat
     3481 ttatgataaa ccgatgatct attacccgct ctctacactg atgttagcgg gtattcgtga
     3541 tattctgatt attagtacgc cacaggatac tcctcgtttt caacaactgt tgggtgatgg
     3601 tagccagtgg gggctaaatc ttcagtacaa agtgcaaccg agtccagatg gtcttgcgca
     3661 ggcatttatc atcggtgaag agtttatcgg tggtgatgat tgtgctttgg ttcttggtga
     3721 taatatcttc tacggtcatg atctgccgaa gttaatggat gtcgctgtta acaaagaaag
     3781 tggtgcaacg gtatttgcct atcacgttaa tgatcctgaa cgctacggtg ttgttgagtt
     3841 tgataaaaac ggtacggcaa tcagcctgga agaaaaaccg ctacaaccaa aaagtaatta
     3901 tgcggtaacc gggctttatt tctatgataa cgacgttgtc gaaatagcga aaaaccttaa
     3961 gccttctgcc cgtggtgaac tggaaattac cgacattaac cgtatttata tggaacaggg
     4021 gcgtttatcc gttgccatga tggggcgtgg ttatgcatgg ctggatacgg ggacacatca
     4081 gagtcttatt gaagcaagca acttcattgc cacaattgaa gagcgccagg gactaaaggt
     4141 ttcctgccca gaagaaattg cttaccgtaa agggtttatt gatgcagagc aggtgaaagt
     4201 attagctgaa ccgctgaaaa aaaatgctta tggtcagtat ttgctgaaaa tgattaaagg
     4261 ttagtaataa aatgaacgtt attaaaacag aaattccaga cgtactgatt tttgaaccga
     4321 aagtatttgg tgatgaacgt ggattctttt ttgaaagttt taaccaaaat atttttgagg
     4381 aagcggtagg tcgcaaagtt gagtttgttc aggataacca ttctaaatct tccaaagggg
     4441 ttttacgtgg tctgcattat cagttagaac cttatgcaca aggaaaactg gtacgctgtg
     4501 ttgtcggtga agtttttgat gttgcggttg atattcgtaa gtcgtcaact acgtttggca
     4561 agtgggttgg tgtgaattta tctgctgaga ataagcgcca gttgtggatc ccagagggat
     4621 ttgcgcatgg gtttttggtg ctgagtgaaa cggcggagtt tttgtataag acgactcagt
     4681 attatcatcc tgaatgtgaa cgagggatca gatgggatga tcagactata aatataaaat
     4741 ggggtaatat agcaagtcat attattttgt ctgaaaaaga tgaaaaggct tgttcattca
     4801 ttgatgcagt atgcttttct taaatgagta tacatgatgt catttaaagt atataaatgg
     4861 agttttattg aaagaatttt agttcaatta ttccagctaa ttataataat tacactttca
     4921 agatttttaa ctccagatga ttttggctta tttggattaa gcgcagtaac tatatcaatt
     4981 gcacaagtat ttgtagatgg tggtttcagt gctgcgctga ttaggaagaa aacacatagt
     5041 gatgttgaac taaacagtgt ttttattatc aacattttta tcgcactttt gctaatatca
     5101 ttagtactcg tatgttctcc actgatagaa aattatttta atattgacgg actagctaat
     5161 ctattaaatc tatcaagttt agtaatatta ataaatgcat tttcagtaat acctaaagta
     5221 gtactgactg tgaatttgaa ttttcgtggt atcgctgagt cttctttaat tgcggttttg
     5281 agcagcggaa tactttccat aatattagct gttaataatt tcggtgccag tacgttagtt
     5341 ttccaactaa tgatttacaa tttgattaac ttaattctta tcatcgccaa atcaaaatgg
     5401 ctaccgagaa aatgttttga tatgcagtca gtaaaagaat tgtgtaattt cagtattaag
     5461 ttatttgcat caggactctt ggatgcatta tatcaaaata tatatctagt tctaataggg
     5521 aaattaatgt ctgttcatga tgttggtatt tttgcacaag caaagaaact ttctgatatt
     5581 ccagcattaa ctattacaac agctctccaa agagcaaacc ttgctgcaac aagcaagctt
     5641 gatgagaatg atgatattaa aaaaagtgtg ctgaagacat tacagttagg tatgtttatt
     5701 atggctccag caatgttttt tataatatca tcgtcagaac cgttagttaa tgtcttactt
     5761 ggtgataatt ggaagagcgt atcaggagta ataacaatac tgtcattatc aggtttatgt
     5821 tatccaattc acgtgctaaa tcaaaatata cttataataa aagggcgatc tgatctccat
     5881 ttaaaattag agatagtaaa aaaatgtgtt ggtctagtac ttatttttat taccatatca
     5941 catggattaa cgtggctatg ctgggggata gtaattagtt ccatactatc aatttttgtt
     6001 aatgcatact attccaataa tcttgttcac gtgacaatat gtgaacaact aaaagtttgt
     6061 ataacgccta tattcttagc aattattagt ttgataatat ctacattatt atgtagtatt
     6121 cctataaact cttgcacatt aatatttctt aatataaaag atgtcttatt atctgccgtg
     6181 acgtttatga ttgtttttgt agttgcaata agttattttt gtagaaaaga aaattttttt
     6241 tgaggaaata aaaatcaaag gtttaacatg attctttcta ttataatacc ttcctataat
     6301 tcggctcatt tattgcttaa tacacttgag agtattagaa aacaaaaatg taaacgaaca
     6361 gatgtagaag ttattgtggt tgatgcggta tctactgacc atacagtcga tattgtaaat
     6421 aaatatggag atattatttc tgattttatt tgtgaaaagg atgaggggat atatgatgca
     6481 atgaataaag gcatagacgt agcgaggggg aaatttagtt attttattgg cgctggtgat
     6541 accttaacag ataatatcct tgcggaaata ttgcctatat taacaaagta tgatgggatt
     6601 gtttatggta atgttataag aactaaaaac aatgcaattt atgatggaga gttcagtatt
     6661 ataaagttat ttcggcggaa tatatgtcac caagctattt tttatcatac agatataatt
     6721 aaagctttaa aatataatac caaatataag gtgttagcag attatcattt aaacatcatt
     6781 ttgttttcga aatttaatat cgctaagaga tatattaatt ttaacattgc ttattatgat
     6841 gatcttgctc agggatttag ccagaacaat agcgatgatg cttttattag tgactattat
     6901 agaataatat gtgaaaacta cagtttaaaa tataaattag ttcttttttg catatcaata
     6961 atccgaaaga taaaaaaggt tataggatga gcaatcagga gtattcgcaa tatttgtttt
     7021 tccctaatat acttttgttt tttactattt ttttattatt atttctgttt tttattgcgt
     7081 ggtttaaaaa taatttatat tcatggttgg atccgctttt attagctata ttattctcaa
     7141 gtgcaggagt atcaactgtc atttttttat atgtacttgg ggaaataaag gttaactatt
     7201 tgttgcaaac tatgctttct cagttaatgt tgatgcttgg atataaaata ttgtctagga
     7261 gagaaacaag ctatttaaat aagcaagtga aacacagtac atatgaacag ttaaatacag
     7321 gatatcctcg cgcgtttctt gcctcgtctt tcttatttat agtcgcgcag gcttcaactt
     7381 attttttatt aggcattcca ttattaatgg ctaatagatt aatgatatat caagaaggtg
     7441 ggtttggtgt attaggacgt atcattgacg caacttttgt tatttcattt tatacatttt
     7501 tagatatgaa ggtacgtttt aaagtacgag ggctttttaa agcatatggg tatatatatc
     7561 tgcttttttt aataatatcc ttggtattaa atggtagcaa agcaggaatc ttaaatttag
     7621 tatttataat aggaatattt tgttacttaa ataaactgaa attcaacata tcgttaataa
     7681 gtatatactc aaaatttgct cgaagattgt tgatatttgc attcatatca atgttcataa
     7741 ttttaatggt aaactatgga attttcagtg atcaaaacta taatcgaata attgatctct
     7801 taggagttat ttttttgagg atctttaatt ccggcgatat atatgtgttg agctatccaa
     7861 atgatgcttt attcaaaata ccagctgaac ataatggtat tatttcgcta tttaaagatt
     7921 tgcttgctac tttccgcatt atcagtacca atgaaatccc accatcatta ggtaatattg
     7981 catttaatat ttactctact actgagattg gtggtgctaa tgcccgtcaa agcttatttg
     8041 gctattacta tttcggtttt atgggatcac ttttgttttc atttttttta ggactaacta
     8101 tgtcattttt taggcatgtt cttcctagaa ttaaaaaagc tacaccattg gcgatgggag
     8161 tgctctgtta ttttattttg aacttttcat ttcttgatat tgatccaagt tatcaatttt
     8221 ctaaattaac gaattttttt attgtaacac cactaataat tttattaatg ggagtcatgc
     8281 cgaatattag gagacagaaa aatgtttagc attataatta taaactggaa ttcttatacg
     8341 gaaacaatga attgtattgc ttcaatattg gcgaacaggg aattgataac tagaattata
     8401 atagttgaca ataattcgag tgatgattca ttaaagaaat taaagcaaga atttatctat
     8461 aatgaccttg taaaaataat agagtcgaaa actaatactg gctactgcgg cggtaataat
     8521 cttggatttc aatattgcct aaacgataat agcaattatt tattagtgtt gaataatgat
     8581 gttattataa ctagacgcac tttaagttta ttaaagaagg catttgaaaa ggatgaaaag
     8641 ttggtaatgg tttcaccgat ggttaatgga gagttaattg aaaactcaac atttcttgta
     8701 aggataaatg aaaatttaaa ttcgacttta gcttcaacac aagatgattc tggttttgta
     8761 tataagtatt atatacctgg atgtgctatt gcattttcaa agaatttttt gaaaaactat
     8821 ggaggattta atgaaaatta cttcatgtat gtagaagaga ttgaaatagc attccgtgca
     8881 cgtaaaaatg gttataatgt agctcaatta acttccttcg attcgtctat attgagaaaa
     8941 aaagatgata tagagaagct tagaaaaaga tatatttggt actatcaaac tagaaatatg
     9001 ctctattttc ttgatgatat atatatgcga aattattgga agggggtttt taaaaaatca
     9061 ttatatataa cattttcttt tatcagacct tttacgcaaa gaaacttagg taatttaatt
     9121 gctgtctata aaggtgtagg tgattacctt aagggagtca aaggtaagca agtcgtttag
     9181 tgaatcgaga gtgttatgaa tattctgatc gtaatgccac atttttaccc ggctgcaaat
     9241 tatggcgggc cagttatttc aagctttaaa acctgtaagg aattagcgaa attaggacac
     9301 aatgtatcag tatctacaag taacgtaaat caaaatggaa agttagatgt aaaattaaat
     9361 actagaatta aaatatctga taatttttac gttaagtatt ataatgaaac gataaaaaat
     9421 agattttcac tcccattaac tttaggtgtg tttagggata ttaaaaattc agatgtggtt
     9481 catattcaat atgtatttaa ctatagtaca gttgttgcat tgttatacag ttatatacta
     9541 cgaaaaaaaa ccattctttc accacgaggc tgtttgggta aatggtgtct ggagcatggc
     9601 agcagactga aaatattctg gaataaattg ttaataaaac cattaacaaa aaatacaata
     9661 tggcatgcaa cttcaaaaca ggaatgtgat gatataatta atcagtttgg tgatgtcaag
     9721 gtaataatat caccaaatgg tattgacctt gaagagtata aagatattac acctataagc
     9781 aaaaaagaat tcatatcacg atatattcca aactattcga gaaatgatta taataaacta
     9841 gtggtttcac tgggccggat acaaaaaaag aaaggttttg atattttaat taagtctttc
     9901 actaacctgt taaatgactt tccagaaagc atattagtaa ttgctggtcc cgatgaaggt
     9961 gaaaaagaaa acctttttga tcttatagct aagaataaaa tgcaggataa tatttttatt
    10021 attgatagca tttatgggcg tgataaattg cattttttaa atgcggctga tgtttttgcc
    10081 ttgccatcac ataatgaaaa ttttggcaat gtttatttag aaagccttgc agcaggaaca
    10141 cctattctgg ctagtaaaca tactccgtgg agtattgttg aacagtttgg atgcggccaa
    10201 tgggtaaata acgacgaagt gtcagttact aatgcgctaa aattattatt tgagggcgat
    10261 gcttatcact tacataaaaa tagtcttaaa tgtgctcaga actattcatg gaaaaattca
    10321 gcggaaatta tttctaatgc attagataac ttaaaatgag ataaaaatga tattatctga
    10381 atataaaaaa atttatcatg cccctaaggt taaatctatt atttggtatg ttttgaactt
    10441 tatatttttt gaaactgcta tcccataccc ttcgcagata aagtgtttta ttctaaaatt
    10501 aatgggtgcg catattggaa agggtgttgt tatcaaacct aatgtgaaaa ttaagtatcc
    10561 gtggaaccta tatatcggaa ataattcttg gttaggtgaa aaagtttgga tcgataacct
    10621 tgatattgtt tcggttggaa ataattgttg catatctcag ggggcttatt tattaacagg
    10681 taatcatgat tatagaagtg aatattttga tttgattatc tcacctataa ctattgaaag
    10741 tgaagtttgg gtgggggcta aagcaattgt tacacctggc gtacatttaa aaacaggtgc
    10801 tattttggcg gttaattctg tcgctgtttc tgatttgaat gaaaactctg tttaccaagg
    10861 taatcctgca acttttaaaa gaaagaggta tagctaatga aaaaagcgtt aatcactgga
    10921 gttactgggc aagatggatc ttacttggca gaattccttc ttgataaagg gtatgaagtg
    10981 catggcatta aacgtcgagc atcttcattt aatacggagc gtgttgatca tatttatcaa
    11041 gatcgccaca atcaaaatcc aaatttcttt cttcattacg gtgatttgac agatacttcg
    11101 aatttgatac gtctcattaa agaaatccaa ccagacgaag tttataacct tggcgctcaa
    11161 tctcatgtag cggtttcatt cgaatcacct gaatacactg ctgacgtaga tgcgatgggg
    11221 actttacgtc tgctggaagc gattcgtatt tgtgggctag agaaaaaaac acgtttctat
    11281 caggcatcaa cttctgaact cttcggctta gttcaggaaa tcccgcagcg agaaacaaca
    11341 ccattctacc ctcgttcgcc ttatgctgtt gcgaagatgt atgcatactg gattactgta
    11401 aactatcgtg aatcctatgg aatgtacgcc tgtaacggta ttctcttcaa ccatgaatcc
    11461 ccgcgtcgtg gtgaaacatt cgtaacacgt aaaatcactc gtgctattgc aaatatttcg
    11521 cagggaatcg aaaaatgtct ctatcttggc aatatggact cactgcgtga ctggggacat
    11581 gcaaaagatt acgtgcgtat gcagtggatg atgttgcagc aagatcaccc agaagatttt
    11641 gttattgcaa caggcaaaca aatttccgta cgtgaatttg ttcgtatgtc cgctaaagaa
    11701 gtaggtctgg agttagaatt ctccggacaa ggtgttgatg aaatagcaac tgttgtcaat
    11761 aaaacatctg actgtgctat tggtgtaagt attggggatg tgatcgttcg tgttgatccg
    11821 cgttattttc gtcctgcaga agttgaaact cttcttggtg atccagcaaa agcgaaaaaa
    11881 gtattgggtt gggaaccaga gattacagtt gaagagatgt gtgcagaaat ggttgccagt
    11941 gatctggcga aagcaaaaca gcatgcactt ctgaaaagcc atggttacga tgttgcagtt
    12001 tctctggagc ggtaaggtat gacaaaaaaa cgtatctacg ttgctggtca ccgaggtatg
    12061 gttggctctg ctatttgccg tcaattatca ctgcgtgatg atatcgaatt agtggtcaaa
    12121 acacacaaag aactcgatct aaccgtacag aaggatgttg atgcattttt tgagcaagag
    12181 aaaattgatc aggtttatct tgctgcggct aaagttggtg gcatttatgc caacaataca
    12241 tttccggcag aattcatcta tcagaatctc atgattgaga gtaatattat tcattcagct
    12301 cacaaggccg gaattcaaaa attacttttt ttaggctcaa gctgtattta tcctaaattc
    12361 gcagagcagc cgatgaacga gtctgcgctt ttaacgggca tacttgagcc aactaatgag
    12421 ccatacgcaa ttgctaaaat tgcgggcata aaattatgtg aatcttataa ccgtcaatat
    12481 ggccgtgatt atcgcagtgt aatgcctact aatctttatg gcataaatga taatttccac
    12541 cctgaaaact ctcatgttat tccggcactc atgcgtagat tccatgaggc aaaagagagt
    12601 ggtgcaccag aagttattgt ctggggaacc ggaacaccga tgcgtgagtt tttatatgtt
    12661 gatgatatgg ctgccgcatc tgttcatgta atggaacttg acgaagcaat ttatcaacaa
    12721 aatacacagc ctatgttatc tcatattaat gttggtacgg gtgtagattg ttctatacgt
    12781 gaaatggctg aaacaatggc ctctgtggtg ggttatcaag gaaaaattgt ttttgatgtt
    12841 actaaacctg atggcactcc gcgtaaactt atggacgtta cccggctcaa aaacttgggc
    12901 tggcaatatc gctataattt acatgaaggc ttatcattaa catataaatg gtttattgag
    12961 aatattaatt cttttcgggg atagttatga acaagagatt ggaacgtgag ctatttaaaa
    13021 caatagttga gcatactcct ctaatctcga ttgatctcat aattagaaac gataaaggag
    13081 aggcgctgct ggggcagcgc ctgaatcgcc cagcacaaaa ttattggttt gtgccaggag
    13141 ggcgaattta taaggatgag tcattcgagg ttgcatttaa acggataaca tttgaagagt
    13201 tgggcgttaa aattagtctt aatgacgcct tatttcttgg ggtgtatgaa catttttaca
    13261 atgataattt ttctgaagca gaattttcta cacactatgt agtgcatgga tatgaaatcc
    13321 aacttaatcc tcagcaactt cacctaccaa cggtccagca taattcctac aagtggtttg
    13381 atgtagtaac gttgcttaat agcactatag ttcatcaata taccaaaaat tattttatac
    13441 caaggtaata catatgctac ttcccgttgt catggccggt ggttcaggta ccagattatg
    13501 gcctctttca cgtacacttt atccgaaaca atttctgtct ttaaatagtc gtttaaccat
    13561 gttgcaagag acattgcggc ggcttgacaa gatcgaacat aaacccgctt tggtcatttg
    13621 taacgaatca catcgcttta tcgttgctga acaattgcgt aaagagggtt taaagcatag
    13681 tggtattttg cttgagcctg tgggtcgtaa tactgcgcct gctgtagcac tcgcagcact
    13741 tcaggctatg gtaactggag atgatcctat tctgttggtt cttgctgctg atcatgaaat
    13801 ccagaatgag gataatttca ttgatgcagt tcttgcagcc aagaattttg cagagcaggg
    13861 taagcttgtt acgtttggta ttgttccaac atccccagag actggctatg gttacattaa
    13921 gtcaggtgaa tctttggatg gaaaaggtta caaagttgcg gcttttgttg aaaaaccaga
    13981 gcttcacgta gctcagcggt atatatcaga tggcggttat ctttggaata gtgggatgtt
    14041 tatgttcaga gcatctgtat ttatcgatga actgaaaaaa ttccgaccag atattttagc
    14101 cagttgccaa acctctctgt cctcttcgac acaagattta gattttatcc gtctggataa
    14161 ctcttcattt tcttgctgtc ctgaagagtc gattgattat gccgttatgg aaaaaacagc
    14221 cgaagctgtc gtcgttccat taaatgcgca atggagtgat gttgggtcat ggtctgcatt
    14281 gtgggaaata agttcaaaag accaaagcgg caatgccatt cgtggtgatg tattggttga
    14341 agatgctaca gatagttatc tttattcgca gcatagactt attggtgccg tgggcgtaaa
    14401 ggatttggtt gttgttgaaa cgaaagatgc cgtattagtt gctcataaag acaaagttca
    14461 gcaagttaaa aatattgtcg ctcaacttaa aaaaaataat cgaacagaat atttacagca
    14521 tcgggaaatt tttcggccct ggggcagtca tgatactata gctgaagggc cgcgctttca
    14581 agtgaaacat gtgattgtat tacctggtca cattaccgct aagcagattc attaccatcg
    14641 tactgagcat tggattgttg tctcggggac agctaaagtt catcttgaag ataagactta
    14701 tcttgtttct gaaaatgaat caacatacat acctgttggt gttccacatg ccattgaaaa
    14761 tcctggcaag atcccactcg aaataattga ggttagatca ggagtctacc ttgaagagga
    14821 tgatgttata agagttcctt cttctggagt aggatactaa tgcgaatttc tattatcaca
    14881 gctacttata atagtgaaaa aactctcctt gatacattac tttctctaga aaagcaaacg
    14941 catccagata ttgaatatat agttgtagat ggagcatcga aagataatac aattaagctg
    15001 attaagagta attgtacaaa agtttcaaaa atcatttgcg aacccgataa gggcatttat
    15061 gatgctctga ataaaggtat tcaagcagct tcgggggatg ttattggttt tttacattct
    15121 gatgacttat ttgcttatga tgatgctatt gcagatatag caaaaacatt tgaaagtaca
    15181 ggatgtgatg ctatttatgg cgatttggag tatgttgccc aaaatgatac gactaaacgt
    15241 attagattat ggaaaagtgg ttcatttagt cgtttcaaga tgaaagtggg ttggatgccg
    15301 ccacacccat cattttatat gaaacgcgaa tgttatagtc agtttggtag tttttcttta
    15361 gattatcgaa tatctgctga ttatgactca ttattacgat atattttaaa acaacgtatt
    15421 tcgatagcat atttaccaaa agtcttagtg aagatgcgtg ttggtggaat tagcaatcgt
    15481 tcagtatctt caatggtcaa gaagtcaatg gaagacattc gtattatgaa acagaatggt
    15541 attatctggc cacttgcttt agcgtataaa aatatatcca aacttcctca atttattaaa
    15601 aagtaatcat catgttaaat gttaaaaaaa tcattaacga tagtaatatt gcgttcggta
    15661 ctagtggtgc tcgcggttta gtgatcgatt tttcccatga tgtttgtgct gcgttcactc
    15721 atgcgtttct ttctgttgtt gatgacaaat acaattttaa taaagttgcc ttagccattg
    15781 ataaccgccc aagtagttac gaaattgctc aggcatgcgc gtatgctata aaacaacatg
    15841 gattcgaggt tgaatatcat ggtgtaattc cgactcctgc attagctcat tattcgatgc
    15901 agaaaaacat tccctgcata atggtcacag ggagtcatat cccttttgat cgtaatggct
    15961 taaaattcta cagacctgat ggtgaaatca ctaaagagga tgagctcgca attataaata
    16021 gtgagtacac attttcgcct gtaggtgaat tacctcatct tgaaacaagc actcaaggtg
    16081 cagactacta cctcgagcgt tatgtttctc tttttaatcc cgagatttta aaggggaaaa
    16141 gaataggggt atatgaacat tctagtgcgg gacgcgattt atatgctcct ctttttaatc
    16201 aaatgggtgc agaggtcatt tccctcggta ggagtgatga gttcgttccg attgatacgg
    16261 aagcagtaag cgatgaagat cgtatacttg caagagagtg gtctaaaaaa tataatcttg
    16321 atgctatttt ctcaacagat ggcgatggtg atcgtccttt agttgccgat gaaaatggtg
    16381 aatggttaag aggcgatatt ctgggactac ttactgctat tgaacttaat atcaaggcgt
    16441 tggctattcc agtgagttgt aatacagcaa ttgaagagtc taataaattt gcaagtatac
    16501 aacgaacgaa aataggttct ccttatgtaa ttgcagcatt tgcagatctt gctaagcaat
    16561 ttgattcagt cgctggtttt gaagctaatg gtggttttct ccttgcctcc gatttacaaa
    16621 ttaatggcaa ggaattaaaa tcgttaccta cacgagatgc tgtgttacca gcattaatgc
    16681 tcttaatagc ttctcgcaat agtactattt ctcaactgat taataatctt cctcagcgat
    16741 tcacttggtc agatagagtt aaaaacttcc cttcagattc aagtcaacaa attataaaaa
    16801 atgccatatc gtcacccaat aatttcttta atagtttagg ttatgaatca ttatcctgtt
    16861 ccgctattga tgaaacggat ggtgcaagat ttactttaaa taatggtgat attatacatc
    16921 tccgtccttc cggtaatgct ccagaactcc gttgttatgc tgaggccagt gatgaaaatc
    16981 aggctaagca atatgttaca aatgtgctgg gaaatattac ctctttgatt tcttgatgtt
    17041 ataggtttat ctacgcttat atgtgtgcgt aggtttgatt acacgtagat gccggtatac
    17101 agaattgaag aacggtattt gttgcattaa tgaaattcag cactacacac attcgtgcaa
    17161 cttgagataa catctcaatc atattcaagt cgcgcataca tcgcggtgaa caccccctga
    17221 caggagtaaa caatgtcaaa gcaacagatc ggcgtcgtcg gtatggcagt gatggggcgc
    17281 aaccttgcgc tcaacatcga aagccgtggt tataccgtct ctattttcaa ccgttcccgt
    17341 gagaagacgg aagaagtgat tgccgaaaat ccaggcaaga aactggttcc ttactatacg
    17401 gtgaaagagt ttgttgaatc tctggaaacg cctcgtcgca tcctgttaat ggtgaaagca
    17461 ggtgcaggca cggatgctgc tattgattcc ctcaaaccat atctcgataa aggcgacatc
    17521 atcattgatg gcggtaacac cttctttcag gacaccattc gtcgtaaccg tgagctttct
    17581 gcagaaggct ttaacttcat tggtaccggt gtttccggcg gtgaagaagg tgcgctgaaa
    17641 ggtccttcca ttatgcctgg tgggcagaaa gaagcctatg gactggttgc accgatcctg
    17701 accaaaatcg ccgcagtagc tgaagacggt gagccatgcg ttacctatat tggtgccgat
    17761 ggtgcaggtc actatgtgaa gatggttcac aacggtattg aatacggcga tatgcagctg
    17821 attgctgaag cctattctct gcttaaaggt ggcctgaacc tcaccaacga agaactggcg
    17881 cagaccttta ccgagtggaa taacggtgaa ctgagcagct acctgatcga catcaccaaa
    17941 gatatcttca ccaaaaaaga tgaagacggt aactacctgg ttgatgtgat cctggatgaa
    18001 gcggctaaca aaggtacggg caaatggacc agccagagtg cgctggatct cggcgaaccg
    18061 ctgtcgctga ttactgagtc tgtgtttgca cgttatatct cttctctgaa agatcagcgt
    18121 gttgccgcat ctaaagttct ctctggcccg caagcacagc cagcaggcga caaggctgag
    18181 ttcatcgaaa aagttcgtcg tgcgctgtat ctgggcaaaa tcgtttctta cgcccagggc
    18241 ttctctcagc tgcgtgctgc gtctgaagag tacaactggg atctgaacta cggcgaaatc
    18301 gcgaagattt tccgtgctgg ttgcatcatc cgtgcgcagt tcctgcagaa aatcaccgat
    18361 gcatacgccg aaaatccgca gatcgctaac ctgctgctgg ctccgtactt caagcaaatt
    18421 gccgatgact accagcaggc gctgcgcgat gtcgtcgctt atgcggtaca gaacggtatc
    18481 ccggttccga ccttcgccgc tgcggttgcc tattatgaca gctaccgcgc cgcagttctg
    18541 cctgcgaacc tgatccaggc acagcgtgac tatttcggtg cgcatactta taagcgcatt
    18601 gataaagaag gtgtgttcca taccgaatgg ctggattaat ctgatttaaa tcaattaatc
    18661 aatgcaaggc ccggagaaac cctccgggct tttttattat acaaagcggc aggttatggc
    18721 ctttttttat aatttatggt taaaaaatcg atataataca gagccgcaca gcaggatcgc
    18781 tgccttgaca gttcatctac atcagcgtta aaaatcccgc agtagatgaa gttgtggtgg
    18841 tggattaatg accactctaa atgtttaacc ggaagaagtc agagctaatg aaaataacaa
    18901 tttcaggaac aggttatgtt ggtctttcaa atggtattct gattgcgcaa aaccacgaag
    18961 tggttgcact ggatatcgtt caggccaaag tggacatgct taacaagaag cagtcaccga
    19021 ttgttgataa ggagattgaa gagtatctgg cgactaaaga tctcaatttc cgcgctacga
    19081 cagataagta tgacgcgtat aaaaatgccg attacgttat tattgccaca cctaccgatt
    19141 atgatccgaa aacaaattat tttaatacct caagcgtgga agcggtcatt cgcgatgtga
    19201 cagaaattaa tcccaacgcg gtgatggtga ttaaatctac aattccggta ggtttcacca
    19261 aatcaattaa agaacgtctg ggtatcgata accttatttt ctctccggaa tttctccgtg
    19321 aaggtaaagc cctttacgat aatctccatc cttcacgtat tgtcattggt gagcgttcag
    19381 aacgcgcaga acgtttcgct gcgttattac aggaaggagc gattaagcaa aatatcccga
    19441 ccctgtttac cgactccact gaagcagaag cgattaaact ttttgcaaac acctacctgg
    19501 cgatgcgcgt ggcgtacttt aacgaactgg atagctacgc agaaagttta ggtctgaatt
    19561 cccgtcaaat aatcgaaggc gtttgtctcg acccacgaat tggcaaccat tacaacaatc
    19621 cgtcgtttgg ttatggtggt tattgtctgc cgaaagatac caagcagtta ctggcgaact
    19681 accagtctgt gccgaataac ctgatttcgg caattgtcga tgctaaccgc acacgtaaag
    19741 actttattgc cgatgccatt ttgtcacgca aaccgcaagt ggtgggtatt tatcgtctga
    19801 ttatgaagag cgggtcagat aacttccgtg cgtcttccat tcaggggatt atgaaacgta
    19861 tcaaggcgaa aggcgttgaa gtgatcatat acgagccagt gatgaaggaa gactcattct
    19921 tcaactctcg cctggaacga gatctcgcca ccttcaaaca acaagccgac gtcattattt
    19981 ccaaccgtat ggcagaagag cttaaggatg tggcagacaa agtctacacc cgcgatctct
    20041 ttggcagtga ctaacatact gttatcaggg cgattttcgc cctgattctc ttatgttccc
    20101 tttgtagtaa ttcattcttt ttatcattta tcctatagca ttcatggcga ttatcgctaa
    20161 actatggcga cttaaaatta tttcgtccgt tagggtaatg atgagagtag aaaataataa
    20221 tgtttctggg caaaaccatg acccggaaca gattgatttg attgatttac tggtgcagtt
    20281 gtggcgcggc aagatgacaa ttatcatctc tgtcattgtc gctattgccc tggctatagg
    20341 gtatctggcc gttgccaaag aaaagtggac ttccacagct atcgttactc aacctgatgt
    20401 ggggcaaatc gctggttata ccaacgcgat gaatgtgatc tacggatcgg ctgtaccgaa
    20461 agtctccgac attcaggctt cgcttatcgg acgttacagc actgcattct cagcattagc
    20521 ggaaacgctg gataaccagg aagaagcaga gaagctgacc attgagccta ccgttaaaaa
    20581 ccagtcgtta ccattggcgg tgtcttatgt tggtgaaaca cctgaaggtg ctcagaagca
    20641 gctggcgaag tatatccagc aagttgacga ccaggtaaat gaagaactgg gaaaagacct
    20701 gaaagacaac atcgcgctgc gtatgaaaaa cttgcaggat tctctgaaaa ctcaggaagt
    20761 agttgcgcag gagcagaaag atctgcgtat ccgtcagatt caggaagcgt tgcagtatgc
    20821 gaatcaggcg caggtgacaa agccacaggt tcagcagact gaagatgtga cacaagatac
    20881 gttgttcctt ttggggagcg aagcgctgga gtcgatgatt aagcatgagg cgacccgtcc
    20941 gttggtgttc tcacctaact actatcagac tcgtcaaaac ctgctggata tcgaaaactt
    21001 aaaggttgat gatcttgata ttcatgctta ccgttatgtg atgaaaccga cgttacctat
    21061 tcgtcgcgat agtccgaaaa aggcaatcac cttgattctg gcggtgctgc tgggcggcat
    21121 ggttggcgcg gggattgtgc tggggcgtaa tgctctgcgt aattacaacg cgaagtaatc
    21181 ttttcggttt taaagaaaaa gggcagggtg gtgacacctt gcccgttttt tttgccggat
    21241 gcgacaacaa tatcgcatcc gcttaccccg caactcactg atgccgttta cgcaggttct
    21301 caatcaccgt cgttaagtcc agatcctgat cctgcaacag caccagcagg tgatacatca
    21361 aatcagatgc ctcgttggtc agctcaaagc ggtcatgtac cgttgcggca agtgcggttt
    21421 ccaccccttc ttcgcccact ttctgcgcaa tgcgtttggt gccgctggca tacagtttgg
    21481 cggtgtagga ggtttccgga tcggcagatt tgcgttcggc gagcaattgt tccagttgat
    21541 acaggaacag ccactggtga gcggtgtcgc cgaagcagct gctggtgcct ttgtggcagg
    21601 tcgggccgat gggattcgcc aataccagta gcgtgtcgtt gtcgcagtcc ggcgcaatat
    21661 ttactacgtt gaggaaattg cccgacgttt cgcctttggt ccacagtcgc tgtttagtgc
    21721 gcgagaagaa ggtgacattg ccgctttcta tggttttgtc cagagcttcc ggattcatat
    21781 agcccagcat taacacttcg ccggataccg catgttgcac aatcaccggc atcagtccgt
    21841 cggttttttc ccagtccagt tcgcggcgtt gttgttctgt taacatatcc tgatctccac
    21901 gccctgtgtt gccaggtacg cttttaattc accaatattg attatttgtt tgtggaatac
    21961 ggaagctgcc agcgcgccgt caacgtcggc atcacggaag gcttcgagga agtgttccat
    22021 ggtgcccgcg ccaccggagg caattagcgg gacgtggcaa acttcacgca cttttttcag
    22081 ttgttcgagg tcgtaaccgt
//
