LOCUS       MN172356               17083 bp    DNA     linear   BCT 24-AUG-2019
DEFINITION  Escherichia coli strain 16-04178 O-antigen biosynthesis gene
            cluster, complete sequence.
ACCESSION   MN172356
VERSION     MN172356.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17083)
  AUTHORS   Lang,C., Hiller,M., Konrad,R., Fruth,A. and Flieger,A.
  TITLE     Whole genome-based public health surveillance of less common STEC
            serovars and untypable strains identifies four novel O genotypes
  JOURNAL   J. Clin. Microbiol. (2019) In press
   PUBMED   31366691
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17083)
  AUTHORS   Lang,C., Hiller,M., Konrad,R., Fruth,A. and Flieger,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2019) Division of Enteropathogenic Bacteria and
            Legionella (FG11), Robert Koch Institute, Burgstrasse 37,
            Wernigerode, Saxony-Anhalt 38855, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: A5 v. 2.1.3
            Assembly Name         :: OgN-RKI
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="16-04178"
                     /isolation_source="stool"
                     /db_xref="taxon:562"
                     /country="Germany"
     misc_feature    1..17083
                     /note="O-antigen biosynthesis gene cluster"
     gene            79..972
                     /gene="galF"
     CDS             79..972
                     /gene="galF"
                     /codon_start=1
                     /transl_table=11
                     /product="GalF"
                     /protein_id="QEE84054.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            1345..2430
                     /gene="rmlB"
     CDS             1345..2430
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="QEE84055.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIHKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            2430..3329
                     /gene="rmlD"
     CDS             2430..3329
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="QEE84056.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDIHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLINATSVEAIAKAANEVGAWAIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3387..4265
                     /gene="rmlA"
     CDS             3387..4265
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="QEE84057.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVQFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKRFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     CDS             4270..4809
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlC"
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="QEE84058.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPESDRGIIWNDPSLKIEWPLD
                     NTPVLSARDKAHSILDMAI"
     CDS             4826..5572
                     /codon_start=1
                     /transl_table=11
                     /gene="orf6"
                     /product="DUF616 domain-containing protein"
                     /protein_id="QEE84059.1"
                     /translation="MSKKYVVYTALFGSYDFLTDISHLSSSDIEYICFTDIECNNNLG
                     WKIVKVSNFSSTPAMMNRYYKLHPHCYFSNYDASLYVDANIKIKSNLNLFFEKYIAKG
                     DFLIPQHEQRNCIFKEAKECIVLKKGNEKIITRQMLNYKKDGMPVGYGLGENNILFRK
                     HNAPEIKKIMADWWEELVKESQRDQLSLAYVMWKNNKKLEFLDETCRNTNDYFEYQTH
                     KKYTNRSVLEKFKDRFFILSRRIKYHRWCV"
     gene            5573..6553
                     /gene="wzy"
     CDS             5573..6553
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="QEE84060.1"
                     /translation="MYKIKKSTLILILGAIILILASGFKPIGIDRDSINYVKMIQSPF
                     LYSDLVSREPTFNLIQFICHILFFNNVAAFFLIYALLGVGLKILAIKRSSDYPVFSFF
                     LYVFFYYILHDFTQIRAGVASALFLLAIYDRVNSNNRQALTKIIFAVCFHYSAIITLF
                     IFLLNNKINRFKYVLLPIMGGLFSLLLTHDTLETVSQYLPYFIGNKINNYLTLQVNSD
                     IYHINVFNVYYSALVFIYLSIIIFIGNKLIYRDIIYVKILGIGLFFFYAMSFVPIFAF
                     RISEFLSVVIIILFANISNYIKEKYTYQLCMFIFGVGYFITQGVMQNINL"
     CDS             6561..7397
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /product="glycosyltransferase"
                     /protein_id="QEE84061.1"
                     /translation="MINFKMLVVLYKESIDSSTTLESIKKVFEHNHLHSNFELLIWDN
                     SPVSLITKEELRIYSDNCGGIKHIEYIHNELNKPLSFIYNFALNKYKDGDYIIILDQD
                     SGFDKGYIEEVINVALEQSPELILPIIYYKNKIVSPSKISYIRGNYYKCIPCGRINPK
                     KISAINSGMIISLDYIKRIAFEYNKYLKNYCTDDYFMRQFRRFGNYAYVLNYTFKHHL
                     SLSTLNDNSELLKERYKLMKEGRYIVYSENLFDKIAIRVYFSIHKIYMAFKYKDISYL
                     KV"
     CDS             7399..8400
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="glycosyltransferase"
                     /protein_id="QEE84062.1"
                     /translation="MDDIVISIPRFSLSGGNLVSLNVALYLRTKGFKVYAVSGFSKKK
                     IEDVVLVKYKKGIMNSFANIICFLNLSVLSLFYKNYIATHHLSAILNFVRPAKFALVQ
                     DLESDFYPMRLRFLGRCLWKSYLKSENLIFTNAYLANKVCGDCQQVDGFAYVDSINEI
                     EYKKDNGQVHRYDALLILRDGKYKGYNNTLRLFEFLNSSGIKTCLVNQSRNSIISKYN
                     ELILNGLPRKDFLKLLCAANYFICLSEWEGLGLPNFEAISLGVKVISNPIPSALIIKD
                     NFPSVISLNTSFNEIRELIKSSGLINNCDVSEFFVAQNKAWLEYVFRLISLETNYAN"
     CDS             8390..9184
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="glycosyltransferase"
                     /protein_id="QEE84063.1"
                     /translation="MQISISVVSHGHFNIIDTLGCIEKLSKKDWVKICIVDNIGETGF
                     DVWCKERNIIYIKNITRLGFGKNNNIAFNKLRNRPDSVENQYFLVLNPDVSITIEQFE
                     KLISEVAENKIRFCCINLFKDQRYSEYDYSIRNYPGLWDFVSSFVFSINKTIVNKDYI
                     NHPQHVDWASGSFLLFDSRLYESLGGFDERYFMYCEDIDICLRARLEKNCKLLYMPEI
                     KAVHFAAHKNRKILSRHFIWHIRSIFRYLIVKNILLAKRYFSNFGN"
     gene            9298..10704
                     /gene="gnd"
     CDS             9298..10704
                     /gene="gnd"
                     /codon_start=1
                     /transl_table=11
                     /product="Gnd"
                     /protein_id="QEE84064.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLSLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWMY"
     gene            10840..12126
                     /gene="wzx"
     CDS             10840..12126
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="QEE84065.1"
                     /translation="MSNYIKNAGWIFCEKFIRIAVGFILFALISRVLNPTEFGLLSYY
                     QTIATMFLAITNLGFDNILINEFNQDKRHNEIFSTAFWSRIVVSIFVIILFSVGLYFD
                     DIALKNKLVLLLCMSSLIFQSQNTYISFFQSQLQASVVTKISFCALIISSIFKSYLLF
                     ISGDMIWFALSYSFDFAVSFLFLVFFSYKRNYISINPKYFRLSVLKRLLRSSWPIIAS
                     SIIIVLYTRLDQLMIMRMLGADSVALFSVALKISEAYLFIPAALVTSYYPLISKSPTK
                     ENIRFYFDVIFSSSIIMAVVVAIASYFVIPYMFGANYIASYDILIILLFGSLFATLGS
                     ACTNLMIVYGLSYLRLVRAVYGLLINFSLNILLIPKYGIIGAAYASFVSQIFATWLSN
                     SLNRKTFECFRIQSMTVLTLGLTGLRQLFTMLFRRG"
     CDS             12136..12603
                     /codon_start=1
                     /transl_table=11
                     /gene="orf13"
                     /product="serine acetyltransferase"
                     /protein_id="QEE84066.1"
                     /translation="MFDSHDRRKGIIRRIAFHFKMKLRECREYYYSIRYYHQFKIPLS
                     NHLTIGIKTLGENKTTLPHPVGIVIGKNVNIGEKCVVYQNVTIGVSNNKYEDYPIIGN
                     NVIIYAGAIIIGKVIIGDNAIIGAGCIVTKDVPEGKIAVGSPMRIIDKKHNNY"
     gene            12701..13867
                     /gene="ugd"
     CDS             12701..13867
                     /gene="ugd"
                     /codon_start=1
                     /transl_table=11
                     /product="Ugd"
                     /protein_id="QEE84067.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSDRAERFAALLQEGAIKQDIPILFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(13933..14937)
                     /gene="wbnF"
                     /note="gla"
     CDS             complement(13933..14937)
                     /gene="wbnF"
                     /codon_start=1
                     /transl_table=11
                     /product="WbnF"
                     /protein_id="QEE84068.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPQWTVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            15289..16323
                     /gene="wzz"
     CDS             15289..16323
                     /gene="wzz"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz"
                     /protein_id="QEE84069.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGP
                     AVPKVSDIQTSLIGRYSTAFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETA
                     EGAQKQLAQYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(16420..17031)
                     /gene="hisI"
     CDS             complement(16420..17031)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="HisI"
                     /protein_id="QEE84070.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLTTVIENLRKRHQ"
BASE COUNT     5073 a   3050 c   3643 g   5317 t
ORIGIN
        1 caattcaagg taattgatgc tggcgaaaaa aatcgaacaa gctataattc agcaaccatt
       61 ttacaggtgg atgaaataat gacgaattta aaagcagtta ttcctgtagc gggtcttggg
      121 atgcatatgt tgcctgccac taaggcgatt cccaaagaga tgctaccgat cgtcgacaag
      181 ccaatgattc agtacattgt tgacgagatt gtggctgcag ggatcaaaga aatcctcctg
      241 gtaactcacg cgtccaagaa cgcggtcgaa aaccacttcg acacctctta tgaattagaa
      301 tctctccttg aacagcgcgt gaagcgtcag ctgctggcgg aagtgcagtc catctgtccg
      361 ccgggtgtga ccattatgaa cgtgcgtcag ggcgaacctt taggtttggg ccactccatt
      421 ttatgtgcac gacccgccat tggtgacaac ccatttgtcg tggtgctgcc agacgttgtg
      481 atcgacgacg ccagcgccga cccgctgcgc tacaaccttg ctgccatgat tgcgcgcttc
      541 aacgaaacag gacgcagcca ggtgctggca aaacgtatgc cgggtgacct ctctgaatac
      601 tccgtcattc agaccaaaga accgctggat cgcgaaggca aagtcagccg catcgtggaa
      661 tttatcgaaa aaccggatca gccgcagacg ctggactcag acatcatggc cgtgggtcgc
      721 tatgtgcttt ctgccgatat ttggccggaa cttgaacgca ctcagcctgg tgcatggggg
      781 cgtattcagc tgactgatgc catcgctgaa ctggcgaaaa aacagtccgt tgatgccatg
      841 ctgatgacag gtgacagcta cgactgcggt aaaaaaatgg gttatatgca ggcatttgtg
      901 aagtatggac tgcgcaacct gaaagaaggg gcgaagttcc gtaaagggat tgagaagctg
      961 ttaagcgaat aatgaaaatc tgaccggatg taacggttga taagaaaatt ataacggcag
     1021 tgaagattcg tggcgaaagt aatttgttgc gaatattcct gccgttgttt tatataaaca
     1081 atcagaataa caacgagtta gcaataggat tttagtcaaa gttttccagg attttccttg
     1141 tttccagagc ggattggtaa gacaattagc gtttgaattt ttcgggttta gcgcgagtgg
     1201 gtaacgctcg ctacatcgta ggcatgcatg cagtgctctg gtagctgtaa agccaggggc
     1261 ggtagcgtgc attaatacct ctattaatca aacaaagagc cgcttatttc acagtatgct
     1321 ctgaagtaat atggaataaa ttaagtgaaa atacttgtta ctggtggcgc aggatttatt
     1381 ggttctgctg tagttcgtca cattataaat aatacgcagg atagtgttgt taatgtcgat
     1441 aaattaacgt acgccggaaa cctggaatca cttgctgatg tttctgattc cgaacgctat
     1501 gtttttgaac atgcggatat ttgcgatgca gctgtaatgg cacggatttt tgctcagcat
     1561 cagccggatg cagtgatgca cctggcagct gaaagccatg tggatcgttc aattacaggc
     1621 cctgcggcat ttattgaaac caatattgtt ggtacttatg tccttttaga ggccgctcgg
     1681 aattactggt ctgctcttga tggcgacaag aaaaatagct tccgttttca tcatatttct
     1741 actgacgaag tctatggtga tttgcctcat ccagatgaag taaataataa cgaagaatta
     1801 cccttattta ctgagacgac agcttacgcg ccaagcagcc cttattccgc atccaaagca
     1861 tccagcgatc atttagtccg tgcgtggaaa cgtacctatg gtttaccgac cattgtgact
     1921 aactgttcga ataactacgg tccttatcac tttccggaaa aattgattcc actagtaatt
     1981 cttaatgctc tggaaggtaa ggcattgcct atttatggta aaggggatca aattcgcgac
     2041 tggctgtatg ttgaagatca tgcacgtgcg ttatataccg tcgtaaccga aggtaaagcg
     2101 ggtgaaactt ataacattgg tggacacaac gaaaagaaaa acatcgatgt agtgctcact
     2161 atttgtgatt tgttggatga gattgtaccg aaagagaaat cttaccgcga gcaaattacc
     2221 tatgttgccg atcgcccggg acacgatcgc cgttatgcga ttgatgcaga gaagattagc
     2281 cgcgaattgg gctggaaacc gcaggaaacg tttgagagcg ggattcataa aacggtggaa
     2341 tggtacctgt ccaatacaaa atgggttgat aatgtgaaaa gtggtgccta tcaatcgtgg
     2401 attgaacaga attatgaggg ccgccagtaa tgaatatcct ccttttcggc aaaacagggc
     2461 aggtaggttg ggaactacag cgtgctctgg cacctctggg taatttgatt gctcttgata
     2521 ttcactccac tgattactgt ggtgatttta gtaatcctga gggtgtagct gaaaccgtaa
     2581 gaagcattcg gcctgatatt attgtcaacg cagccgctca caccgcagta gacaaagcag
     2641 aatcagaacc ggagtttgca caattaatta acgcgacgag tgtcgaagcg atcgcgaaag
     2701 cagccaatga agtcggcgcc tgggctattc actactctac tgactacgta tttccgggaa
     2761 caggtgaaat accatggcag gaggcggatg caaccgcacc gctgaacgtt tatggagaaa
     2821 ccaagttagc cggagaaaaa gcattacaag agcattgtgc gaagcacctt attttccgga
     2881 ccagctgggt ctatgcaggt aaaggaaata acttcgccaa aacgatgttg cgtctggcaa
     2941 aagagcgcga agaactagcc gttattaatg atcagtttgg tgcgccaacg ggcgcagagt
     3001 tgctggctga ttgtacggca catgcaattc gtgttgcact gaataaacca gaagtcgcag
     3061 gcttgtatca tctggtagcc agtggtacca caacttggca cgattatgct gcgctggttt
     3121 ttgaagaggc gcgcaaagca ggcattcccc ttgcactcaa caagctcaat gcagtaccaa
     3181 caacagccta tcctacacca gctcgtcgcc cccataactc tcgtcttaat acagaaaaat
     3241 ttcagcagaa ctttgcgctt gtcttgcctg actggcaggt tggtgtgaaa cgaatgctca
     3301 acgaattatt tacgactaca gcaatttaat agtttttgca tcttgttcgt gatggtggag
     3361 caagatgaat taaaaggaat gatgaaatga aaacgcgtaa aggtattatt ttagcgggtg
     3421 gttctggtac acgtctttat cctgtgacta tggctgtcag taaacagctg ttaccgattt
     3481 atgataaacc gatgatctat tacccgctct ctacactgat gttggcgggt attcgcgata
     3541 ttctgattat cagtacgcca caggatactc ctcgttttca acaactgctg ggtgacggga
     3601 gccagtgggg gctaaatctt cagtacaaag tgcaaccgag tccagatggt cttgcgcagg
     3661 catttatcat cggtgaagag tttatcggtg gtgatgattg tgctttggtt ctaggtgata
     3721 atatctttta cggtcacgat ctgccgaagt taatggatgt cgctgttaac aaagaaagtg
     3781 gtgcaacggt atttgcctat cacgttaatg atcctgaacg ctacggtgtc gttcagtttg
     3841 ataaaaacgg tacggcaata agcctggaag aaaaaccgtt acaaccaaaa agtaattatg
     3901 cggtaaccgg gctttatttc tatgataacg acgttgtcga aatggcgaaa aatcttaagc
     3961 cttctgcccg cggtgaactg gaaattaccg atattaaccg tatttatatg gaacaggggc
     4021 gtttatccgt tgccatgatg gggcgtggat atgcatggct ggacacggga acacatcaaa
     4081 gtcttattga agcaagcaac tttattgcaa caattgaaga acgtcaggga ctgaaagttt
     4141 cctgtccgga agaaattgct taccgtaaac ggtttattga tgctgagcag gtgaaagtat
     4201 tagccgaacc gctgaagaaa aatgcttatg gtcagtatct gctcaaaatg attaaaggtt
     4261 attaataaaa tgaacgtaat taaaactgaa attccagacg tactgatttt tgaaccgaaa
     4321 gtttttggtg atgagcgtgg tttctttttt gagagcttta accagaaggt ttttgaggaa
     4381 gctgtaggcc gcaaagttga atttgtccaa gataaccatt cgaagtcttg taaaggtgtt
     4441 ttacgcgggc tgcattatca gttagaacct tatgcacaag ggaaattggt gcgctgcgtt
     4501 gttggtgaag tttttgacgt agctgttgat attcgtaaat cgtcaccaac ttttggcaaa
     4561 tgggttgggg tgaatttatc tgctgagaat aagcgacaat tgtggatacc tgaagggttt
     4621 gcgcatggat ttttggtgtt gagtgaaacg gcggagtttt tgtataagac gacgaattat
     4681 tatcatccag agagtgatag agggattatt tggaatgatc caagtttaaa aattgagtgg
     4741 cccttggata atactcctgt actctctgca agagataagg cacactcgat cttagatatg
     4801 gctatttgat ttaagggaaa taattgtgtc taaaaaatat gttgtctata cggcactatt
     4861 tgggagttac gattttttaa cagatatctc acacctatct tcatctgata ttgagtatat
     4921 ttgttttact gatatcgaat gcaataataa tttggggtgg aaaattgtta aagttagcaa
     4981 cttcagttca actcctgcta tgatgaatcg ttattacaaa ttgcatcctc attgttattt
     5041 ttctaactat gatgccagct tatatgttga tgctaatata aaaataaaat caaatttaaa
     5101 tctttttttt gaaaaatata tagcgaaagg cgatttttta atacctcagc atgaacagag
     5161 aaattgtatt tttaaagaag ctaaagagtg tattgtttta aaaaaaggta atgaaaaaat
     5221 aataacccgg caaatgttaa attataaaaa ggatgggatg ccagtaggtt atggcttagg
     5281 tgagaataat attctgttca gaaagcataa tgctccagag ataaaaaaaa tcatggcaga
     5341 ttggtgggag gagttggtta aagagtctca acgagatcaa ttgagtttag cttatgttat
     5401 gtggaaaaat aataaaaaac tagagttttt agatgaaact tgtaggaaca ctaatgatta
     5461 ctttgaatat caaacacaca aaaaatatac aaatagaagc gtattggaaa aatttaaaga
     5521 taggtttttt attttgtcaa gacgtattaa atatcatcgt tggtgtgtat aaatgtataa
     5581 gataaaaaaa tccacattaa ttcttatttt aggggctatt attttaattt tagcctctgg
     5641 gtttaaacct atcggtatag accgtgattc tattaattat gtgaaaatga ttcaatcacc
     5701 atttctctat agtgatttgg ttagcagaga gcccactttt aatctgattc agtttatatg
     5761 tcatatatta ttttttaaca atgttgctgc attttttttg atttatgcat tacttggtgt
     5821 aggactaaag atattagcaa taaagaggtc atcagattat cctgttttta gtttttttct
     5881 gtatgttttt ttttattata tcttacatga ttttactcag attcgtgctg gtgtggcatc
     5941 ggcactattc ttacttgcta tttatgatag agttaattcg aataaccgac aagcgttgac
     6001 taaaatcatt tttgcagtat gttttcatta ttcagcaata ataacgcttt ttatattttt
     6061 attgaataat aaaattaata gatttaaata tgtgttattg ccaattatgg gtgggctatt
     6121 ttctttgtta ttaacccatg acacgcttga aactgttagt caatatttgc cttacttcat
     6181 aggtaataaa ataaataatt atcttacctt acaagttaac agtgatatat atcacataaa
     6241 tgtttttaat gtttattatt ctgcgctagt atttatctat ttatcgatta ttatttttat
     6301 tggaaataaa ctaatatatc gagatattat atatgttaaa attttaggta ttgggttgtt
     6361 tttcttttat gcaatgtcat ttgttcctat ctttgcgttt agaatttcag agtttttaag
     6421 tgtggtaata attattttat tcgcaaatat atcaaattac attaaagaaa aatacacgta
     6481 tcaactatgt atgtttattt ttggtgttgg ttattttatc actcaaggtg taatgcaaaa
     6541 tatcaatttg tagaatatgt atgataaatt ttaaaatgct agttgtgctt tataaagaaa
     6601 gtattgattc ttctacaaca ttggagtcaa taaaaaaagt tttcgaacat aatcatttac
     6661 attcaaactt cgaactttta atttgggata acagccccgt atctttaata actaaagaag
     6721 aattacgtat ctattcagat aattgtggtg gaattaaaca tatagaatat atacacaatg
     6781 aattgaataa gcctttatct tttatttata attttgcatt aaataaatat aaggatggag
     6841 attatattat tatattggat caggattcgg gttttgacaa gggatatatt gaagaggtaa
     6901 taaatgttgc tttggaacaa tctcctgagt tgatattgcc aataatttat tacaaaaata
     6961 aaattgtaag cccatctaaa ataagttata taagaggtaa ttattataaa tgcattccat
     7021 gtggtaggat taatccaaaa aaaataagcg caattaacag tggcatgatt atatctctcg
     7081 actacataaa gagaattgcg tttgaataca ataaatattt aaaaaattat tgcacagatg
     7141 attatttcat gcgtcagttt agaaggtttg gtaattatgc atatgtcttg aattatactt
     7201 ttaaacatca tttgtcactt tctactctaa atgataattc ggagctgttg aaagagcgtt
     7261 ataagcttat gaaggaggga cgttatattg tttattctga aaatctattt gataaaatag
     7321 caataagagt ttatttttcc atccacaaga tttacatggc atttaaatat aaagatatta
     7381 gttatttaaa ggtttaaaat ggacgatata gtgatctcaa tacctcgatt ctcattatct
     7441 ggtggaaact tagtttcgtt aaatgtagcc ttatatctac gaacgaaagg ctttaaggta
     7501 tatgctgttt caggtttttc gaaaaaaaaa atagaagatg tggttttagt gaaatataaa
     7561 aaagggataa tgaactcatt tgcaaatatt atttgctttc ttaatttatc agttctttcc
     7621 cttttttata aaaattatat tgccacacac catctttcag caatactgaa ttttgttcgc
     7681 ccagctaaat ttgcattggt ccaagatctt gagtctgatt tttatccaat gaggcttcgt
     7741 tttttgggac gatgtttgtg gaaaagttat ttgaaatcag agaatcttat cttcacaaat
     7801 gcttatcttg ctaataaagt atgtggagat tgtcaacagg tggatggttt cgcatatgtc
     7861 gactcaatta atgagattga atataaaaaa gacaatggtc aagtacatag gtatgatgct
     7921 ttacttatct taagggatgg aaagtataaa ggatacaata atacactaag gttgtttgag
     7981 tttttaaatt cttcaggtat taaaacatgc cttgttaatc aatcaaggaa cagtataata
     8041 tcaaaatata atgaattaat tctgaatggt cttccaagaa aagatttttt aaaattactt
     8101 tgtgcggcaa actattttat ctgtctttct gaatgggagg ggttgggatt accaaatttt
     8161 gaggcgatta gtcttggagt aaaagtaata tcgaatccta ttccttcggc tctaattata
     8221 aaagataatt tccccagtgt catttctctt aacacaagtt ttaatgaaat tagagagttg
     8281 attaagagtt ctggattaat taataattgc gatgtatctg aattttttgt tgcgcaaaat
     8341 aaagcttggt tagagtatgt atttagatta atcagtttgg aaacaaatta tgcaaattag
     8401 tatctcagta gtatctcatg gtcattttaa tattattgac acattaggct gcattgaaaa
     8461 actatccaaa aaagactggg tgaaaatttg tattgtagat aatattggtg aaactggatt
     8521 tgatgtatgg tgtaaagaac gcaatataat atatatcaag aatattacaa ggttaggttt
     8581 tggaaagaat aataacatag ctttcaataa gttaaggaat agacctgatt ctgtagaaaa
     8641 ccaatacttt ttagtattaa atcctgatgt aagcataact atagaacaat ttgaaaaatt
     8701 aatttcagag gttgctgaaa ataaaatacg tttttgctgc attaatcttt ttaaagatca
     8761 gcgttatagc gaatatgatt attctataag gaactatcct gggttatggg attttgtctc
     8821 gtcatttgta ttttcaataa ataaaactat agttaataag gattacataa atcatcctca
     8881 acatgtggat tgggcatctg gatctttctt attattcgat tcaaggttat acgagtcctt
     8941 ggggggattt gatgagagat attttatgta ctgcgaagat attgatattt gtcttcgtgc
     9001 aagattagaa aaaaactgta aattgttata tatgccagaa ataaaggcgg tacattttgc
     9061 tgcgcataaa aataggaaga tattatccag acattttatt tggcatatca gaagtatatt
     9121 ccggtatctc atcgtaaaaa atattcttct tgcaaaaaga tatttttcta attttggtaa
     9181 ctaacaaata aatatagggt gttagaaatt ttaaattctg taactggaat gtttttatat
     9241 gctttattat tttaaagcga atacacagtg gtaaccaatc cctgacagga gtaaacaatg
     9301 tcaaagcaac agatcggcgt cgtcggtatg gcagtgatgg ggcgcaacct tgcgctcaat
     9361 atcgaaagcc gtggttatac cgtctctatt ttcaaccgtt cccgtgaaaa gactgaagaa
     9421 gtgattgccg aaaatccagg caagaaactg gttccttact atacggtgaa agagtttgtt
     9481 gaatctctgg aaacgcctcg tcgcatcctg ttaatggtga aagcaggtgc aggcacggat
     9541 gctgctattg attccctcaa gccatacctc gataaaggtg acatcatcat tgatggtggt
     9601 aacaccttct tccaggacac cattcgtcgt aaccgtgagc tttctgcaga aggctttaac
     9661 tttatcggta ccggtgtttc cggtggtgaa gaaggtgcgc tgaaaggtcc ttccattatg
     9721 cctggcggac agaaagaagc ctatgaactg gttgcgccga tcctgaccaa aatcgccgca
     9781 gtggctgaag acggcgaacc gtgcgttacc tatattggtg ccgatggtgc aggtcattat
     9841 gtgaagatgg ttcacaacgg tattgaatac ggtgatatgc agttgattgc cgaagcctat
     9901 tctcttctta aaggtggcct tagtctttcc aacgaagaac tggcgcagac gtttaccgag
     9961 tggaataacg gtgaactgag cagctacctg atcgacatca ccaaagatat cttcaccaaa
    10021 aaagatgaag acggtaacta ccttgttgat gtgattctgg atgaagcagc aaacaaaggt
    10081 acgggcaaat ggaccagcca gagtgcgctt gatctcggcg aaccgctgtc gctgattacc
    10141 gagtctgtgt ttgcacgtta tatctcttct ctgaaagatc agcgtgttgc cgcatctaaa
    10201 gttctctctg gcccgcaagc acagccagca ggcgacaagg ctgagttcat cgaaaaagtt
    10261 cgccgtgcgc tgtatctggg caaaatcgtt tcttacgccc agggcttctc tcagctgcgt
    10321 gctgcgtctg aagagtacaa ctgggatctg aactacggcg aaatcgcgaa gattttccgt
    10381 gctggctgca tcatccgtgc gcagttcctg cagaaaatca ccgatgccta tgccgaaaat
    10441 ccgcagatcg ctaacctgct gctggctccg tacttcaagc aaattgccga tgactaccag
    10501 caggcgctgc gcgatgtcgt cgcttatgcg gtacagaacg gtatcccggt tccgaccttc
    10561 gccgctgcgg ttgcctatta tgacagctac cgcgccgcag ttctgcctgc gaacctgatc
    10621 caggcacagc gtgactattt cggtgcgcat acttataagc gcattgacaa agaaggtgtg
    10681 ttccataccg aatggatgta ttgattcttt tttagttatt aaagagcccg cttttgtggc
    10741 tctttaatat atacaaaact cataattcag tcgattcaac ctagacaaga cgattatttt
    10801 tattttttct taatgctaag agcctacgtg agatattaga tgtctaacta tatcaaaaat
    10861 gctggttgga ttttttgcga aaaatttata cgaatagccg tgggttttat attatttgca
    10921 ttaatttcaa gagtgcttaa ccctaccgaa tttggcttgc tctcttatta tcaaacaata
    10981 gcaaccatgt tcttagcaat aactaattta ggttttgata atatcctgat taacgaattc
    11041 aatcaggata aaaggcataa tgaaatattt tcaacagcat tctggtcaag aattgttgtt
    11101 tcgatttttg taataatatt attttctgtg ggattgtatt ttgatgatat cgcgctaaag
    11161 aataaattag ttttactatt gtgtatgagt agtttgatct ttcaaagcca aaatacatac
    11221 atatcatttt ttcaatctca attacaagcc tcagttgtga caaaaattag tttttgtgca
    11281 cttattatat cctcgatatt taaaagttac ttgttgttta taagtggaga tatgatatgg
    11341 tttgctttat catacagttt tgattttgct gtttcttttt tatttcttgt ttttttttct
    11401 tataagagaa attatatatc cattaatcca aaatatttcc gattaagtgt actaaaacgt
    11461 ttgttacgtt catcttggcc aattattgca tcatcaataa ttattgttct ttatactcgc
    11521 ttagatcaat taatgattat gcgaatgtta ggggcagatt cagttgcatt atttagtgtt
    11581 gctttaaaaa tatcggaagc atatttattc attcccgcag cgctagttac atcatattat
    11641 ccattgattt caaaatcacc gaccaaagaa aacatcaggt tttattttga tgtaattttt
    11701 tcgagctcca tcataatggc tgtagtggta gctatagcat cttattttgt aataccatat
    11761 atgtttgggg ctaattatat tgcatcttat gatatattaa ttattttgct ttttggctca
    11821 ttatttgcta cactcggttc agcatgtaca aatttaatga tagtgtatgg tttatcttat
    11881 ttacggttag ttcgagctgt gtatggatta ttaataaact tttccttaaa tattttactt
    11941 attcctaaat atggtattat tggtgccgct tatgcatctt ttgttagtca gatatttgca
    12001 acttggctaa gcaatagcct taatagaaaa acgtttgaat gcttcagaat acagtcaatg
    12061 accgttctaa ctttgggtct aacaggatta agacaattgt ttacaatgct atttagaagg
    12121 gggtaggtat attatatgtt tgacagtcat gacagaagga aaggcataat acgaagaata
    12181 gcttttcatt ttaaaatgaa attgagagaa tgtcgtgaat actattatag tattagatat
    12241 tatcatcagt ttaaaattcc actctcgaac catcttacca ttggcatcaa aacattaggt
    12301 gaaaacaaaa ccactttacc tcatccggta ggaatagtta taggtaagaa tgttaatatc
    12361 ggtgaaaaat gtgttgttta tcaaaatgtc actatagggg tgagtaataa taaatatgag
    12421 gattatccca ttattgggaa taatgttatt atttatgcgg gagcaattat aataggtaaa
    12481 gtaattatag gggataatgc aataattggt gcaggttgca ttgttacgaa agatgtacct
    12541 gaaggaaaga ttgcagttgg ttcaccaatg agaattatcg ataaaaaaca taacaactat
    12601 taatgatctc agtgaattct taaaaatttt aaaatgtatt gactataatg ttcttttttt
    12661 gctattaata tgaaaatttt cattaaataa gaatgcagat atgaaaataa caatttcagg
    12721 aacaggttat gttggtcttt caaatggtat tctgattgcg caaaaccacg aagtggttgc
    12781 actggatatc gttcaggcca aagtggacat gcttaacaag aagcagtcac cgattgttga
    12841 taaggagatt gaagagtatc tggcgactaa agatctcaat ttccgcgcta cgacagataa
    12901 gtatgacgcg tataaaaatg ccgattacgt tattattgcc acacctaccg attatgatcc
    12961 gaaaacaaat tattttaata cctcaagcgt ggaagcggtc attcgcgatg tgacagaaat
    13021 taatcccaat gcggtaatga ttataaaatc aactatccct gttggtttta cagagtccat
    13081 taaagaacgg tttggtattg aaaatgtgat cttttcgccg gagtttttac gcgaagggaa
    13141 agcactttat gataacttgc atccatcacg cattgtgatt ggtgagcaat ccgatcgcgc
    13201 tgaacgtttt gcagcgttat tacaggaagg cgctattaag caagacatac caatattgtt
    13261 tactgactca accgaggctg aggcgattaa actttttgcc aatacttatc tggcgatgcg
    13321 tgtggcttat tttaatgaac tagatagtta cgctgaaagt ctgggtctta actcccgcca
    13381 aattattgag ggggtttgcc ttgatcctcg tatcggtaat cactacaaca atccctcatt
    13441 tggttatggt ggttattgtc tgccgaaaga taccaagcag ttactggcaa actatcaatc
    13501 tgtaccgaat aatctgattt cggcaattgt tgatgctaac cgcacgcgaa aagactttat
    13561 tgccgattct atccttgcac gtaaaccgaa agttgttggc gtttatcgtt tgattatgaa
    13621 gagtggttca gacaatttcc gtgcttcctc gattcagggt attatgaagc gaatcaaggc
    13681 gaaaggtgtg ccagtaatcg tttatgagcc agcgatgaaa gaggacgatt ttttccgttc
    13741 gcgcgtggta cgtgatctgg atgcgttcaa acaagaagct gatgttatta tttctaaccg
    13801 tatgtctgcc gatctggctg atgtagcaga taaagtttat acgcgcgact tgtttggcaa
    13861 tgattaatta ttttgtttta ttctaagaaa aggccctgat atattagggc cttttcttat
    13921 ggttttgtaa aatcaaactt tatagaagtt acgataccat tctacaaagt tctttacccc
    13981 ctctttaact gacgtttcag gtttgaatcc tattacgtca tacagtgctt ttgtatcagc
    14041 actggtttcc agtacatcac cgggttggag aggcatcata ttcttgttgg cttcaatacc
    14101 cagagcttct tctaaagcat tgatatagtc catcaactcc acaggcgaac tattaccaat
    14161 gttatagaca cgatatggtg ctgaacttgt tgcaggcgat ccagtttcta cagtccactg
    14221 tggatctttt tctggaataa catcctgtaa gcgaataata gcttcggcaa tatcatcaat
    14281 ataagtaaag tcacgcttca ttttgccgaa gttgtaaaca tcaatgcttt taccttccag
    14341 catggcttta gtaaatttaa ataatgccat atccggacgt ccccatggac cataaaccgt
    14401 aaagaaacgc agccctgtgg tcggtaagcc atacaaatga gaatacgtat gggccatgag
    14461 ttcattcgct tttttagttg ctgcataaag cgaaacagga tgatctacag agtcatctgt
    14521 agagaaaggc atcttgcggt tcatgccata aacagaactg gaggaagcgt aaagtagatg
    14581 ctgaacatta ttatggcggc atccttccag tatgttcagg aatccaatca ggtttgcatc
    14641 tgcatatgca ttgggatttt caagggagta acgtacaccg gcttgcgcag cgaggtttat
    14701 tacgcgatcg aaccgctcgt ctgcaaacag tgccgccatt ttctcgcgat cggccaggtc
    14761 aattttataa aaactgaaat tgtcgtgctt gagtaaatca agtcgtgctt gtttgaggtt
    14821 gacatcgtaa taatcattta agttgtcaat ccctacaacc tgatgaccag ctgcaagaag
    14881 ccgtttactt acatagaaac cgataaagcc agcagctccc gtaaccagaa atttcattta
    14941 taatcctcac tcaggctaga atatagccaa ttttcatctg gcataactga aagttaaatc
    15001 ataccgttag acaagaaaaa aagataatcg gtatcagttc taaacctggc tgttttttct
    15061 ggtaacgtgc tcattttaca atcaaagctg ttctaagctg actatacaag ccgacgtcat
    15121 tatctccaac cgtatggcag aagagcttaa ggatgtggca gacaaagtct acacccgcga
    15181 tctttttggc agcgactaac atcttgttat cagggcgatt ttcgccctga ttctcttatg
    15241 ttccctttgt aataattcat tatttttatc atttatccta tagcattcat ggcgattatc
    15301 gctaaactat ggcgacttaa aattatttcg tccgttaggg taatgatgag agtagaaaat
    15361 aataatgttt ctgggcaaaa ccatgacccg gaacagattg atttgattga tttactggtc
    15421 caactatggc gcggcaagat gacaattatc atctctgtca ttgtggctat tgccctggct
    15481 atagggtatc tggccgttgc caaagaaaag tggacttcca cagctatcgt tactcaacct
    15541 gatgtggggc aaatcgctgg ttataccaac gcgatgaatg tgatttacgg tccggctgta
    15601 ccgaaagtct ccgacattca gacttcactt atcggacgtt acagcactgc attctcagca
    15661 ttagcggaaa cgctggataa tcaggaagaa cctgaaaaac tgaccattga gcctaccgtt
    15721 aaaaatcagt ccttaccttt agcggtttct tatgttggtg aaacagctga aggtgcgcag
    15781 aaacagctgg cgcagtatat ccagcaagtt gacgaccagg taaacgaaga actggaaaaa
    15841 gacctgaaag acaacatcgc actgcgtatg aaaaacttgc aggattcgct taagacccag
    15901 gaagtggttg cgcaggagca gaaagatctg cgtatccgtc agattcagga agcgttgcag
    15961 tatgcgaatc aggcgcaggt gacaaaacca caggttcagc agactgaaga tgtgacgcaa
    16021 gatacgttgt tccttttggg gagcgaagcg ctggagtcga tgattaagca tgaggcgacc
    16081 cgtccgttgg tgttctcatc aaactactat cagacacgtc aaaacctgct ggatatcgaa
    16141 agcttaaagg ttgatgatct tgatattcat gcttaccgct atgtaatgaa accgacgtta
    16201 cctattcgtc gcgatagtcc gaaaaaggca atcaccttga ttctggcggt gctgctgggc
    16261 ggcatggttg gcgcggggat tgtgctgggg cgtaacgctc tgcgtaatta caacgcgaag
    16321 taatcttttc ggttttaaag aaaatgggca gggtggtgac accttgcccg ttttttttgc
    16381 cggatgcgac aacaacatcg catctgctta ccccgcaact cactgatgcc gtttacgcag
    16441 gttctcaatt accgtcgtta aatccagccc ctgatcctgc aacagcacca gcaggtgata
    16501 catcaaatca gacgcctcgt tggtgagctc aaagcggtca tgtactgtcg ctgccagtgc
    16561 ggtttccacg ccttcttcgc ccactttctg cgcaatgcgt ttggtgccgc tggcatacag
    16621 tttggcggtg taagaggttt ccgggtcggc agatttacgt tcggcgagca gttgttccag
    16681 ttgatacaaa aacagccact ggtgagcggt gtcgccgaag cagctgctgg tgcctttgtg
    16741 gcaggttggg ccgatgggat tcgccagcaa cagtaatgtg tcgttgtcgc agtccggggc
    16801 aatactcacc acattgagga aattgcccga cgtttcgcct ttggtccaca gtcgctgttt
    16861 agtgcgcgag aagaaggtga ctttgccgct ttcgatggtt ttgtctaagg cttccgggtt
    16921 catatagccc agcattaaca cttcgccgga taccgcgtgt tgcacaatca ccggcatcag
    16981 tccgtcggtt ttttcccagt ccagttcgcg acgttgttgt tctgttaaca tatcctgatc
    17041 tccacgccct gtgttgccag gtacgctttt aattcaccaa tat
//
