LOCUS       LC125927               14272 bp    DNA     linear   BCT 04-JUN-2016
DEFINITION  Escherichia coli DNA, O-antigen biosynthesis gene cluster, strain:
            121862.
ACCESSION   LC125927
VERSION     LC125927.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Seto,K., Nishii,H., Ohnishi,M., Mekata,H.,
            Ogura,Y. and Hayashi,T.
  TITLE     Six Novel O Genotypes from Shiga Toxin-Producing Escherichia coli
  JOURNAL   Front Microbiol 7, 765 (2016)
   PUBMED   27242776
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14272)
  AUTHORS   Iguchi,A., Iyoda,S. and Seto,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2016) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuenkibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..14272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="121862"
                     /db_xref="taxon:562"
                     /note="O genotype:OgN1; serogroup:O40"
     gene            <1..906
                     /gene="wcaM"
     CDS             <1..906
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAU71553.1"
                     /translation="NYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILI
                     SDHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVE
                     NGKHFVIRNIKACNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVI
                     KGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEVKHASLELHNK
                     PQHLFMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKEETLLSLANVHAVNERGQSS
                     VDIDRINHHIVNVEKINFRLPERRE"
     gene            1064..2059
                     /gene="gne"
     CDS             1064..2059
                     /gene="gnu"
                     /note="ogN1_1"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAU71554.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            2302..3195
                     /gene="galF"
     CDS             2302..3195
                     /gene="galF"
                     /note="ogN1_2"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAU71555.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLNE"
     gene            3387..4637
                     /gene="wzx"
     CDS             3387..4637
                     /gene="wzx"
                     /note="ogN1_3"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAU71556.1"
                     /translation="MLRNILYLFGMQGTNYLLPLIILPYLVRVLGPEQYGRLGFSVAI
                     IQYLILLIDFGFNLLSSARIAKNKENTFIVSKIYSATIFAKVCISIIIIFITILLTFF
                     VPKLYDIRWLLAVSVIQIWGAIFTPIWFFQGMEVMKKFAIITVIARFISLPLIFVFVK
                     KPSDVLIAAFLQSCVFAISGVIAFILIRQMGVRLVKIKLSTISSYLKRSSGIFVGTVA
                     ISLYTLSTAVILGLVASNYDVGIFNAADKIRAALLGVFVMLGSVFYPRINNLWKFNRN
                     GAYDILRKIIIFQSLASLLLMVLFYFSVPLIVRYYLGEQYYDCIRLLKVMAPMIFLVP
                     ASIIMSNYILLPFGYRKYFSYIPVITAFMHFFYAFYLSKNYGAYGACIAILLTELISF
                     MILLVVNLKLHTFKYIFGFEYEPR"
     CDS             4624..5568
                     /note="ogN1_4"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf5"
                     /product="glycosyltransferase"
                     /protein_id="BAU71557.1"
                     /translation="MNRDKVTVLLPVYNCESYVDECIKSIRDQTYKNLEILIINDGSN
                     DGSEHIILKHAKQDERIIYIHQPNRGLIETLNYGLDIATGKYIARIDADDVCLPNRIE
                     SQYLHMEATKADICGGNYYEINDSGDIIRKRFVPFDRVCMELSLVSRVPFAHPTVMFR
                     NSFLIENNVRYRKNANVYAEDLELWCRLKNLGASFSNTKDFLIKYRVLNTSLSRLNKQ
                     KIFFDSMRFTTEYYIKHHEYLLGLLLNEGMAASLNPEEEEIVLSFIYKEILFKHNLSI
                     LYLLRKFKFKQAIKSLLRELKHYKYRKVIIGVTSESIL"
     gene            5552..6802
                     /gene="wzy"
     CDS             5552..6802
                     /gene="wzy"
                     /note="ogN1_5"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAU71558.1"
                     /translation="MKASYDSKIYFPKVLLILFFLYNVNYKFIPGDIPTSYLSLGFIV
                     VASLYWILRKKRFPVANGWALVSALLLFFCSMISYFDNIEHADLYMIRTTFIYLIMVL
                     FVSPFIACIFKNDRKEVLKTVGYAGLVNGILILGMLIFKPLQYIYLPLLSEKTFLLIG
                     GNDAIESLMSLRMIGITGFSAYTTGFVQVLCAICYIYYMILRDGRIRLKLSDYILLII
                     IFLSALVSARSSLIGIFLSIIILMFNMNSLRFIKTLSLSIISVIFLFSIITMLLPDNL
                     SDFFINWATEFFVSGTKTGSLQTNIDMYIYGLNDFSAFGQSRWYGDNNDYFMNTDVGW
                     YRLAFSIGFLGVIFWYITLMNIFRFNRLFTSRISIENIISICIFIYITIMMFKGAIIF
                     DSFQSVLILLVLDIVFYNRNKYEA"
     CDS             7241..8413
                     /note="ogN1_6"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="glycosyltransferase"
                     /protein_id="BAU71559.1"
                     /translation="MNIGLYCNWKVKPHKNGGFYIPSVHAKYILAFKKIYKKIFLLSN
                     VGSDEINDSDVLIVDDDIHLIELPGFKNYLSALRFSKQIINGINELTEQCKYIYVRTP
                     EPLCWFFMFAKNKDKIIINHHFTSNPLDVLLKQKSFFSLKKYIKYIAFLPEYYLSIIS
                     ACVNNATCNGPSVLKNIPFFLRKRIRILIENTLTKEELVEKEYCERTHAGCYNFICVS
                     RLQEGKGLQELISAFSAFLNKHIDIDAHLTIIGDGPLKKCLEVQVATLSVSNRITIQG
                     YVQNGEPLNEFYRNAHFLINPSLSETGPRVILEAMSEGCVCLSTDVGYVRYILSQEPV
                     FDSLIIRSDFERQFCLIAKELCVNKELYTELSMKSFFIAQKYSLDSFVENIFIGQE"
     CDS             8417..9169
                     /note="ogN1_7"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /product="glycosyltransferase"
                     /protein_id="BAU71560.1"
                     /translation="MHLVSIIMPTYNSSSTIHQSIESVISQTYTNWELLITDDCSSDD
                     TYKIICNYALKDHRIRVFQNNINSGAGLTRNNSIEHARGRFIAFLDSDDMWTNDKLEK
                     QIKFMLDNHFPLTYTYYKKTNASGEITGRLRPPLEVNYNELLKSNVIGCLTAIYDASS
                     LGKVYMPTIRKRQDMGLWLNILKIVPTAHCLAEELAIYREGHESLSSNKLKILRTQWL
                     FYREHVGLSFIKTLWYFSHYVIRALKKHKLPS"
     gene            9291..10697
                     /gene="gnd"
     CDS             9291..10697
                     /gene="gnd"
                     /note="ogN1_8"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAU71561.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            10919..12112
                     /gene="ugd"
     CDS             10919..12112
                     /gene="ugd"
                     /note="ogN1_9"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAU71562.1"
                     /translation="MFNRKKSELMKITISGTGYVGLSNGILIAQNHEVVALDIVQAKV
                     DMLNKKQSPIVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFN
                     TSSVEAVIRDVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYD
                     NLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVA
                     YFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQS
                     VPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRI
                     KAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRD
                     LFGSD"
     gene            12202..13236
                     /gene="wzz"
     CDS             12202..13236
                     /gene="wzz"
                     /note="ogN1_10"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAU71563.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGP
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSDALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(13332..13943)
                     /gene="hisI"
     CDS             complement(13332..13943)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAU71564.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPQALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4147 a   2668 c   3099 g   4358 t
ORIGIN
        1 aattacgcca tcttacgcca gggatttcat aaccagatta tcggtgccaa catcaccaac
       61 tgtaagttca gcgacttaca gggggacgct attgaatgga acgtggcgat taacgacagt
      121 gatattttga tctccgacca tgtcatcgag cgcatcaact gtaccaacgg caaaatcaac
      181 tggggcatcg gtataggcct tgcgggaagc acttacgata ataattaccc ggaagaccag
      241 gcagtgaaaa actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat
      301 gttgaaaatg gtaaacattt tgttattcgt aatatcaaag cctgcaatat cacgccggat
      361 ttcagtaaga aagcaggtat tgataacgcc acagtcgcta tttacggttg tgacaatttc
      421 gtgattgata atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt
      481 aaaggcaaat atctctcgat accgcaaaat ttccgagtga ataatattca actggataac
      541 acccatcttg cttataaatt gcgcggtatc caaatctctg ccgggaatgc cgtctccttt
      601 gtggcactga ctaacattga ggtgaagcat gcgtcgcttg agctacacaa taagccgcaa
      661 catcttttta tgcgaaatat caaagtgatg caggaatcct ctgttggacc cgcattgagc
      721 atgaacttcg acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaga agaaacactg
      781 ctgtctcttg caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac
      841 aggattaatc accatattgt taatgtggaa aagattaact ttagattgcc ggaacggaga
      901 gagtagattt gcgaccattc ataaacttac tggatataat gttctaaaag attataatcc
      961 tcacactaac tgttaaagct ggggctgggc agcattgact actcagggaa ggtactcatc
     1021 tttatcttgt gtttcttacg taacaaccag actaaagtga tttatgaacg ataacgtttt
     1081 gctcatagga gcttccggat tcgtaggaac ccgactactt gaaacggcaa ttgctgactt
     1141 taatatcaag aacctggaca aacagcagag ccacttttat ccagaaatca cacagattgg
     1201 cgatgttcgt gatcaacagg cactcgacca ggcgttagcc ggttttgaca ctgttgtact
     1261 actggcagcg gaacaccgcg atgacgtcag ccctacttct ctctattatg atgtcaacgt
     1321 tcagggtacc cgcaatgtgc tggcggccat ggaaaaaaat ggcgttaaaa atatcatctt
     1381 taccagttcc gttgctgttt atggtttgaa caaacacaac cctgacgaaa accatccaca
     1441 cgaccctttc aaccactacg gcaaaagtaa gtggcaggca gaggaagtgc tgcgtgaatg
     1501 gtataacaaa gcaccaacag aacgttcatt aaccatcatc cgtcctaccg ttatcttcgg
     1561 tgaacgcaac cgcggtaacg tctataactt gctgaaacag atcgctggcg gcaagtttat
     1621 gatggtgggc gcagggacta actataagtc catggcttat gttggaaaca ttgttgagtt
     1681 tatcaagtac aaactgaaga atgttgccgc aggttatgag gtttataatt acgttgataa
     1741 gccagatctg aacatgaacc agttggttgc tgaagttgaa caaagtctga acaaaaagat
     1801 cccttctatg cacttgcctt acccactagg aatgttgggt ggatattgct ttgatatcct
     1861 gagcaaaatt acgggcaaaa aatacgctgt cagctctgtg cgcgtgaaaa agttctgcgc
     1921 aacaacacag tttgacgcaa cgaaagtgca ttcttcaggt tttgtggcac cgtatacgct
     1981 gtcgcaaggt ctggatcgaa ctctgcagta tgaattcgtt catgccaaaa aagacgacat
     2041 aacgtttgtt tctgagtaat gcagacgagt cagagaaaaa aggaccaggt tggactacga
     2101 ttattcggaa agaggtactg attaaattat cagtatgcgg aattccgagt aatccgagta
     2161 acccagatgc tgatggtgtg tagtgttatc ctaaggttta agtaaagtta tgcggaacta
     2221 agctttggta tatagccata acgtttggct aacctatggc aataatttac gcactatcag
     2281 ccacttagag gtaggaaaag aatgacgaat ttaaaagcag ttataccggt agcaggtctt
     2341 gggatgcata tgttgcctgc cactaaggcg attcccaaag agatgctacc gatcgtcgac
     2401 aagccaatga ttcagtacat tgttgacgag attgtggctg cagggatcaa agaaatcctc
     2461 ctggtaactc acgcgtccaa gaacgcggtc gaaaaccact tcgacacctc atatgaatta
     2521 gaatctctcc ttgagcagcg cgtgaagcgt caactgcttg cggaagtgca gtccatctgt
     2581 ccaccgggcg tgaccattat gaacgtgcgt cagggcgaac ctttaggttt aggccactcc
     2641 attttgtgtg cacgacccgc cattggtgac aacccatttg tcgtggtgct gccagacgtt
     2701 gtgatcgacg atgccagcgc cgacccgctg cgctacaacc ttgcagccat gattgcgcgc
     2761 ttcaacgaaa cgggccgcag ccaggtgctg gcaaaacgta tgccgggcga tctctctgaa
     2821 tactccgtca ttcagaccaa agaaccgctg gatcgcgaag gtaaagtcag ccgcatcgtc
     2881 gaatttatcg aaaaaccgga tcagccgcag acgctggact cagacatcat ggccgttggc
     2941 cgttatgtgc tttctgccga tatttggccg gaacttgaac gcactcaacc tggtgcatgg
     3001 gggcgtattc agctgactga tgccattgct gaactggcga aaaaacagtc cgttgatgca
     3061 atgctgatga ctggtgacag ctacgactgc ggtaaaaaaa tgggctatat gcaggcgttt
     3121 gtgaagtatg ggctacgcaa cctgaaagaa ggggcgaagt tccgcaaagg gattgagaag
     3181 ctactgaatg aataatttga agcctgaatg attctgagga aaatggtgat tattttaatc
     3241 accactgatt ttacaatagg ttagtatatg ttcctaaaat ttaactcttt gagctctttg
     3301 gttgctatag tcaaggcggt agcgtgcatt ataaataatc ctagagctta tatttattat
     3361 ttaggtaaga gcattggaac aatgaaatgt taaggaatat tctttatctt tttggaatgc
     3421 agggaaccaa ttatttactg ccgctaataa tacttcctta tttagtacgt gttcttggac
     3481 cagagcagta cgggcgatta ggattttcag ttgccataat acagtatttg atattgttaa
     3541 tcgactttgg gtttaattta ctatcgtcag ccagaatagc aaagaataaa gagaatacat
     3601 ttatcgtaag taaaatatat tctgcgacta tctttgccaa agtgtgtata tcaataatta
     3661 taatctttat tactatactt ctgacatttt ttgtccctaa attatatgat atacgttggt
     3721 tgttggctgt ttcggttatt caaatatggg gggcaatatt tacgccaatt tggttttttc
     3781 aggggatgga agtgatgaag aaatttgcta ttattactgt tatagcaagg tttatttcat
     3841 taccgcttat tttcgttttt gtcaagaaac caagtgatgt tttaatagcc gcttttcttc
     3901 agtcgtgcgt ctttgctata tccggagtga ttgcgtttat tttaattaga cagatgggag
     3961 ttagactagt taaaattaaa ttgtcgacta tttccagtta tctcaaaaga agtagtggca
     4021 tttttgtcgg aactgtagct ataagtctat atactctcag tactgctgta attttaggcc
     4081 ttgttgcatc aaattatgat gtaggtattt ttaatgcagc agataaaatt agagcagcat
     4141 tacttggtgt gtttgtaatg ttaggtagtg ttttttatcc aaggattaat aatctatgga
     4201 agttcaacag aaatggggca tatgatattt taaggaaaat cataatattt cagtcattag
     4261 catcattact gcttatggtt ttgttttatt tttcagttcc acttatagtt cgttattatt
     4321 taggtgagca atattatgat tgtattagat tgttaaaagt catggcgcct atgattttcc
     4381 ttgttccagc ctctataata atgtctaact atattttatt gccttttgga tataggaaat
     4441 attttagtta cattccagtt attactgcct ttatgcattt cttttatgca ttttatttgt
     4501 caaaaaatta tggggcctat ggcgcatgta ttgcaatatt attaacggaa ttaattagct
     4561 ttatgatttt acttgtagtt aatttaaaat tgcatacctt caaatatata tttgggtttg
     4621 aatatgaacc gagataaagt tacagtctta ttgccagtgt ataattgtga atcttatgtt
     4681 gatgagtgta ttaaatctat tcgcgatcaa acatataaaa accttgagat attaataatt
     4741 aatgatggtt caaatgatgg ttcagaacat attatactta aacacgctaa gcaagatgaa
     4801 cggattatat atattcacca gccaaatcga gggttgattg aaacattaaa ttatggctta
     4861 gatatagcca ctggaaaata tattgcccgg atcgatgctg atgacgtctg tttgcccaac
     4921 agaattgaaa gtcaatattt acatatggag gcgacgaaag cagatatatg tggaggaaat
     4981 tactacgaga taaatgattc aggtgatatt atccgcaagc gttttgttcc ttttgataga
     5041 gtttgtatgg aactctctct ggtaagcaga gttccatttg cacatccgac agtcatgttt
     5101 agaaatagct tcttgataga gaataatgtt cgctatagga agaatgcaaa tgtatatgca
     5161 gaagatttag agttatggtg tagattaaaa aatttgggag catcattttc taatacgaaa
     5221 gactttttaa taaaatatag agtgttaaat acttcgcttt ctcgcttgaa taagcaaaaa
     5281 atattttttg attcaatgag gtttacgact gaatattata ttaagcacca tgagtatttg
     5341 ctgggtttgc tattaaatga agggatggca gcttcattaa atccagagga agaagaaata
     5401 gttctttcat ttatttataa ggagattctt tttaagcata atttatctat tttatatctt
     5461 cttaggaaat ttaaatttaa acaagcaatc aaatctttat tacgtgaatt gaaacattac
     5521 aaataccgca aagtaattat aggtgtaaca agtgaaagca tcttatgatt caaagatata
     5581 ttttcctaaa gttttattaa ttctgttttt tttgtataat gtgaattata aatttatacc
     5641 aggcgacata cctacaagct atttaagttt aggttttatt gtagtagctt ctttatactg
     5701 gatacttagg aaaaaaaggt tccctgttgc caatggttgg gcgcttgtta gcgcattact
     5761 tttatttttt tgttcaatga ttagctattt tgataatata gaacatgctg atttatacat
     5821 gattagaaca acatttatct atcttattat ggtgctgttt gtatcgccat ttattgcttg
     5881 tatatttaaa aatgaccgaa aggaagtatt aaaaacggta gggtatgcag ggttggtaaa
     5941 tggtattttg atactgggta tgcttatatt taagccattg caatatatat atttacctct
     6001 gttaagtgaa aaaacgttct tgctaatcgg aggaaatgat gcaattgaaa gtttgatgtc
     6061 tttacgtatg attggcatca cggggttttc tgcgtataca accggttttg tccaggtgtt
     6121 atgtgcaatt tgttatatat attatatgat actcagagat ggacgaataa ggctaaaatt
     6181 gtctgattat atcttgttga ttattatttt tttatcggca ttggtttctg cacgttcgtc
     6241 tctgattggt atttttctgt cgatcataat tcttatgttt aatatgaatt cattaagatt
     6301 tataaaaaca ttatcattat caattatcag tgttattttt ttatttagca ttataactat
     6361 gttgttgcca gataatctga gtgatttttt cattaattgg gctactgaat tttttgtttc
     6421 tggtaccaaa actggctcat tacaaactaa tattgatatg tacatctacg gtttgaatga
     6481 ttttagtgcg tttggtcaat ccaggtggta tggtgataat aatgattatt ttatgaacac
     6541 agatgttgga tggtataggc ttgcattttc aattggtttt ttgggcgtaa ttttctggta
     6601 tataacacta atgaatattt ttagattcaa caggttgttt acatctagaa tttctattga
     6661 aaatataatt tcaatttgta tatttatata cattactata atgatgttca aaggggcgat
     6721 tatatttgat agttttcaat ctgtcttgat tttattggtt ttagatattg tattttacaa
     6781 caggaataaa tatgaagcat aagtttttat taatgtattc attttttgta aggttaatat
     6841 ttttatggtt gccagatacc ccgatgataa tgcgatttcg gggttatgtt tactcatggg
     6901 gcatgaataa atgtgggcat aatctccaag tttcatctac tgcagtgcta cgtggaatcg
     6961 aacggatatc ctgtgggaat gatgtatatt ttggtcccaa ttcatatatt atggcacgta
     7021 aatcaataat aattggtgat gaaacattag ttgctatgaa tgtcgtgatt gtcgatgcta
     7081 atcatggtaa agaaaataat agttatagat ttaaaagggg aagtgcaaag gagattaata
     7141 tagggcgagg ggtatggatt gcagcaaact gtgttgttac tgcaggttca actataaaag
     7201 atggtacatt aattccacct tgttcagtgg ttaggtaatt atgaatattg gattatattg
     7261 taattggaaa gttaaaccgc ataagaatgg tgggttttat atacctagtg ttcatgctaa
     7321 atatatcctt gcatttaaga aaatatataa aaaaatattt ttactgtcaa atgttggtag
     7381 tgatgaaata aatgattctg atgttctaat tgtcgatgat gatatacact taatagagtt
     7441 gcctggtttc aaaaattatt tatcagcttt aaggtttagt aagcaaatta ttaatggtat
     7501 aaatgaactt accgaacagt gtaaatatat atatgtaagg actccagagc ctttatgttg
     7561 gttttttatg tttgcgaaaa acaaagataa aattataata aatcatcatt ttacatctaa
     7621 tccattagat gttcttctta aacaaaaatc atttttttcc cttaaaaaat acataaaata
     7681 tatagcgttt cttccagagt attatctttc gattatttct gcatgtgtaa ataatgccac
     7741 atgtaatggc ccgtcagttc tgaaaaatat ccctttcttc ttaagaaaaa gaattcgaat
     7801 tcttattgaa aatacgctta caaaagaaga attagtagaa aaagaatatt gtgaaagaac
     7861 acatgcaggc tgttataatt ttatttgtgt tagtcggtta caagaaggta agggacttca
     7921 agaactcatc tctgcatttt ctgctttctt aaataaacat attgatattg atgctcatct
     7981 aactattatt ggggatggac ctttaaagaa atgtttagaa gttcaagttg ctactttatc
     8041 agtctccaat cgaattacta tacaaggata tgtgcaaaat ggagaaccac ttaatgaatt
     8101 ttataggaat gctcattttt taataaatcc ttctttatct gaaactggac cgagagtaat
     8161 tttggaggcg atgtctgaag gatgtgtttg cctgtcaact gatgttggtt acgtgcgcta
     8221 catcttaagc caagaaccag tttttgattc tttaataata cgctctgatt ttgaaagaca
     8281 attttgctta attgcaaaag aattatgtgt aaataaagaa ttatatactg aattatcgat
     8341 gaaatccttt tttattgcac aaaaatattc attggactca tttgttgaga atatattcat
     8401 cggacaggag tagtaaatgc atcttgtttc aataattatg ccaacatata attcgtcatc
     8461 gactatacat cagagtattg aatctgtaat ttcgcaaacc tatacaaatt gggaattgct
     8521 aataactgat gattgctctt ctgacgatac ttacaaaatt atctgcaatt acgcactcaa
     8581 agaccataga attagagtat tccagaataa cataaactct ggtgctggcc tgactagaaa
     8641 taactctatc gaacatgctc gcggaagatt tattgctttt ctcgatagcg atgatatgtg
     8701 gactaatgat aaacttgaaa aacaaataaa attcatgttg gataatcatt ttcctttaac
     8761 ctacacctat tataaaaaga ctaatgcatc tggagaaatc accggcagat taaggccacc
     8821 attagaagtt aactataacg aactattaaa atctaacgtt attggatgtt tgactgcaat
     8881 atatgatgct tcctcattgg gcaaagtata tatgcctaca attcgtaaac gtcaggatat
     8941 gggattatgg cttaatatcc taaaaatagt accaactgct cattgtttgg ctgaggaatt
     9001 ggctatttat agagaggggc acgaaagcct ttcatcgaat aaattaaaga tcctacgaac
     9061 tcaatggtta ttttatagag agcatgtcgg gttgagtttt attaaaacct tatggtattt
     9121 ttcacactat gttattagag cattaaaaaa acataaatta ccaagctaac tcgaaattga
     9181 ataactcctt attgctattt tgaaaactag cagcataata ttaatacaca tacgtagatt
     9241 catttgtcta taccgcattc tcccgcggta acccctgaca ggagtaaaca atgtcaaagc
     9301 aacagatcgg cgtcgtcggt atggcagtga tggggcgcaa ccttgcgctc aacatcgaaa
     9361 gccgtggtta taccgtctct attttcaacc gttcccgtga gaaaacggaa gaagtgattg
     9421 ccgaaaatcc aggcaagaaa ctggttcctt actatacggt gaaagagttt gttgaatctc
     9481 tggaaacgcc tcgtcgcatc ctgttaatgg tgaaagcagg tgcaggcacg gatgctgcta
     9541 ttgattccct caagccatac ctcgataaag gtgacatcat cattgatggt ggtaatacct
     9601 tcttccagga caccattcgt cgtaatcgtg agctttctgc agaaggcttt aacttcattg
     9661 gtaccggtgt ttccggcggt gaagaaggtg cgctgaaagg tccttccatt atgcctggtg
     9721 ggcagaaaga agcctatgaa ctggttgcac cgatcctgac caaaatcgcc gcagtggctg
     9781 aagacggcga accgtgcgtt acctatattg gtgccgatgg cgcaggtcac tatgtgaaga
     9841 tggttcacaa cggtattgaa tacggtgata tgcagctgat tgctgaagcc tattctctgc
     9901 taaaaggtgg cctgaacctc accaacgaag aactggcaca gacctttacc gagtggaata
     9961 acggtgaact gagcagctac ctgatcgaca tcaccaaaga tatcttcacc aaaaaagatg
    10021 aagacggtaa ctacctggtt gatgtgattc tggatgaagc ggctaacaaa ggtaccggta
    10081 aatggaccag ccagagtgcg ctggatctcg gcgaaccgct gtcgctgatt accgagtctg
    10141 tgtttgcacg ttatatctct tctctgaaag atcagcgcgt tgccgcgtct aaagttctct
    10201 ctggcccgca agcgcagcca gcaggcgaca aggctgagtt catcgaaaaa gttcgccgtg
    10261 cgctgtatct gggcaaaatc gtttcttacg ctcagggctt ctctcagcta cgcgccgcgt
    10321 ctgaagagta caactgggat ctgaactacg gtgaaatcgc gaagattttc cgtgctggct
    10381 gcatcatccg tgcgcagttc ctgcagaaaa tcaccgatgc atatgccgaa aatccgcaga
    10441 tcgctaacct gctgctggct ccgtacttca agcaaatcgc cgatgactac cagcaggcgc
    10501 tgcgcgatgt cgtcgcttac gcggtacaga acggtatccc ggttccgacc ttcgccgctg
    10561 cggttgccta ttatgacagc taccgcgccg cagttctgcc tgcaaacctg atccaggcac
    10621 agcgtgacta tttcggtgcg catacttata agcgcattga taaagaaggt gtgttccata
    10681 ccgaatggct ggattaatct gatttaaatc aattaatcaa tgcaaggccc ggagaaaccc
    10741 tccgggcttt tttattatac aaagcggcag gttatggcct ttttttataa tttatagtta
    10801 aaaacgcgat ataatacagc gccgcacagc aggatcgctg ccttgacagt tcatctacat
    10861 cagcgttaaa aatcccgcag tagatgaagt tgtggtggtg gattaatgac cactctaaat
    10921 gtttaaccgg aagaagtcag agctaatgaa aataacaatt tcaggaacag gttatgttgg
    10981 tctttcaaat ggtattctga ttgcgcaaaa tcacgaagtg gttgcactgg atatcgttca
    11041 ggccaaagtg gacatgctta acaagaagca atcaccgatt gttgataagg agattgaaga
    11101 gtatctggcg actaaagatc tcaatttccg cgctacgaca gataagtatg acgcgtataa
    11161 aaatgccgat tacgttatta ttgccacgcc taccgattat gacccgaaaa caaattattt
    11221 taatacctca agcgtggaag cggtcattcg cgatgtgaca gaaattaatc ccaacgcggt
    11281 aatggtgatt aaatctacaa ttccggtagg tttcaccaaa tcaattaaag aacgtctggg
    11341 tatcgataac cttattttct ctccggaatt tctccgtgaa ggtaaagctc tttacgataa
    11401 tctccatcct tcacgtattg tcattggtga gcgttcagaa cgcgcagaac gttttgctgc
    11461 gttgttacag gaaggggcga ttaagcaaaa tatccctaca ttgtttaccg actccactga
    11521 agcagaagcg attaaacttt ttgctaatac ttatctggcg atgcgcgtag catactttaa
    11581 tgaactggat agctatgcag aaagtttagg gctgaatact cgccagatta tcgaaggcgt
    11641 ttgtctcgat ccgcgtattg gcaaccatta caacaatcct tcgtttggtt atggtggtta
    11701 ttgtctgccg aaagatacca agcagttact ggcgaactac cagtctgtgc cgaataacct
    11761 gatttcggca attgtcgatg ctaaccgcac acgtaaagac tttattgccg atgccatttt
    11821 gtcacgcaaa ccgcaagtgg tgggtattta tcgtctgatt atgaagagcg gttcagataa
    11881 cttccgcgcg tcttccattc aggggattat gaagcgtatc aaggcgaaag gcgttgaagt
    11941 gatcatctac gaaccggtga tgaaagaaga ttcattcttc aactctcggc tggaacgtga
    12001 tctcgccact ttcaaacaac aagccgatgt cattatttcc aaccgtatgg cagaagagct
    12061 taaggatgtg gcagacaaag tctacacccg cgatctcttt ggcagtgact aacatcctgt
    12121 tatcagggcg attttcgccc tgattctctt atgttccctt tgtaataatt cattattttt
    12181 atcatttatc ctatagcatt catggcgatt atcgctaaac tatggcgact taaaattatt
    12241 tcgtccgtta gggtaatgat gagagtagaa aataataatg tttctgggca aaaccatgac
    12301 ccggaacaga ttgatttgat tgatttactg gtgcagttgt ggcgcggcaa gatgacaatt
    12361 atcatctctg tcattgtggc tattgccttg gctatagggt atctggccgt tgccaaagaa
    12421 aagtggactt ccacagctat cgttactcaa cctgatgtgg ggcaaatcgc tggttatacc
    12481 aacgcgatga atgtgattta cggtccggct gtaccgaaag tctccgacat tcaggcttca
    12541 cttatcggac gttacagtac tgcattctca gcattagcgg aaacgctgga taaccaggaa
    12601 gaagcagaaa agctgaccat tgagcctacc gttaaaaacc agtcgttacc attggcggtg
    12661 tcttatgttg gtgaaacacc tgaaggtgcg cagaagcagc tggcgaagta tatccagcaa
    12721 gttgacgacc aggtaaatga agaactggaa aaagacctga aagacaacat cgcgctgcgt
    12781 atgaaaaact tgcaggattc tttgaagact caggaagtag ttgcgcagga gcagaaagat
    12841 ctgcgtatcc gtcagattca ggaagcgttg cagtatgcga atcaggcgca ggtgacaaaa
    12901 ccacaggttc agcagactga agatgtgacg caagatacgt tgttcctttt ggggagcgac
    12961 gcgctggagt cgatgattaa gcatgaggcg acccgtccgt tggtgttctc gccaaactac
    13021 tatcagactc gtcaaaacct gctggatatc gaaagcttaa aggttgatga tcttgatatt
    13081 catgcttacc gctatgtaat gaaaccgacg ttacctattc gtcgcgatag tccgaaaaag
    13141 gcaatcacct tgattctggc ggtgctgctg ggcggcatgg ttggcgcggg gattgtcttg
    13201 gggcgtaacg ctctgcgtaa ttacaacgcg aagtaatctt ttcggtttta aagaaaaagg
    13261 gcagggtggt gacaccttgc ccgttttttt gccggatgcg acaacaacat tgcatccgct
    13321 taccccgcaa ctcactgatg ccgtttacgc aggttctcaa ttaccgtcgt taaatccaga
    13381 tcctgatcct gcaacagcac tagcaagtga tacatcaagt cagatgcttc gttggtcagt
    13441 tcaaagcggt catgcaccgt tgctgccagt gcggtttcca cgccttcttc gcccactttc
    13501 tgcgcaatgc gcttggtgcc gctggcatac agtttggcgg tgtaagaggt ttccgggtcg
    13561 gcagatttac gttcggcgag cagttgttcc agttgataca ggaacagcca ctggtgagcg
    13621 gtgtcgccga agcagctgct ggtgcctttg tggcaggtcg ggccgatggg attcgccagc
    13681 aacagtaacg tgtcgttgtc gcagtccggc gtaatattta ctacgttgag gaaattgccc
    13741 gacgtttcgc ctttggtcca cagtcgctgt ttagtgcgcg agaagaaggt gactttgccg
    13801 ctttcgatgg ttttgtccag agcttgcggg ttcatatagc ccagcattaa cacttcaccg
    13861 gataccgcgt gttgcacaat caccggcata agtccgtcgg ttttttccca gtccagttcg
    13921 cgacgttgtt gttctgttaa catatcctga tctccacgcc ctgtgttgcc aggtacgctt
    13981 ttaattcacc aatattgatt atttgtttgt ggaatacgga agctgccagc gcgccgtcaa
    14041 cgtcggcatc gcggaaggct tcgaggaagt gttccatggt gcccgcgcca ccggaggcaa
    14101 tcagcgggac gtggcaaact tcacgcactt ttttcagttg ttcgaggtcg taaccgttac
    14161 gcacgccgtc ctgattcatc atgttgagga cgatttctcc ggcaccgcgt ttttgcactt
    14221 cctctaccca gtcgagcgtt tcccactgag tgatgcgggt gcggctttca tc
//
