LOCUS       LC125931               21020 bp    DNA     linear   BCT 04-JUN-2016
DEFINITION  Escherichia coli DNA, O-antigen biosynthesis gene cluster, strain:
            110062.
ACCESSION   LC125931
VERSION     LC125931.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Seto,K., Nishii,H., Ohnishi,M., Mekata,H.,
            Ogura,Y. and Hayashi,T.
  TITLE     Six Novel O Genotypes from Shiga Toxin-Producing Escherichia coli
  JOURNAL   Front Microbiol 7, 765 (2016)
   PUBMED   27242776
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21020)
  AUTHORS   Iguchi,A., Iyoda,S. and Seto,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2016) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuenkibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..21020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="110062"
                     /db_xref="taxon:562"
                     /note="O genotype:OgN12; serogroup:OUT"
     gene            466..1860
                     /gene="wcaM"
     CDS             466..1860
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAU71615.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPDKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     NHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQMDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHVV
                     NVEKINFRLPERRE"
     gene            2023..2928
                     /gene="galF"
     CDS             2023..2928
                     /gene="galF"
                     /note="OgN12_1"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAU71616.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDNYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKSIEKLLSE"
     gene            3301..4386
                     /gene="rmlB"
     CDS             3301..4386
                     /gene="rmlB"
                     /note="OgN12_2"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAU71617.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPHAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQVRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            4386..5285
                     /gene="rmlD"
     CDS             4386..5285
                     /gene="rmlD"
                     /note="OgN12_3"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="BAU71618.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPKFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPDVAGLYHLVAS
                     GTTTWYDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTIAI"
     gene            5343..6218
                     /gene="rmlA"
     CDS             5343..6218
                     /gene="rmlA"
                     /note="OgN12_4"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAU71619.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKG"
     gene            6227..6781
                     /gene="rmlC"
     CDS             6227..6781
                     /gene="rmlC"
                     /note="OgN12_5"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="BAU71620.1"
                     /translation="MNIIKTEIQDVLIFEPEVYEDERGFFMESFNQRILEKNICKNVK
                     FVQDNHSKSMQGVLRGLHYQLSPFAQGKLVRCTVGRVFDVAVDLRRTSETFGHYVSFE
                     LSAENKKILWIPEGFAHGFLVLSDVAEFQYKTTNYYSPKHERSIIWNDPILNIHWPIL
                     NESDLIISQKDAGAILFNNAEYFD"
     gene            6790..8028
                     /gene="wzx"
     CDS             6790..8028
                     /gene="wzx"
                     /note="OgN12_6"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAU71621.1"
                     /translation="MDKKLLKNIFSLLCVQGVSYLIPLITLPYLVKTLGPHSYGVLGF
                     SLAIWQYFIMLTDYGFNLTATQRIAQYPDDKYKVSLVFWEVMICKLILMLVCGIVIII
                     LLRFTSILSGYESVFFAGGGLLLGNILYPIWLFQGKESMALSSIVNVTTRLSTIPLTF
                     LLVNSVDDAWVSALINSTTSILGGGVALLIVYHKKWLILVTPHKKGLKKQFLEGYHVF
                     MSTAAINLYTTSVTVILGIISGPIVVGYFVAADKLRQAVQGIIVPFSQACYPRIVSLM
                     QKNRKDGLQFIRKMLILQGGGGGILSMFLFVMTPDIINLMYGDEYYRSVITLQILSLC
                     PVLVAISNVLGIQTMLTLGYKREFFLILLISSLFCITLLYPLCIHLMENGAALSVVLS
                     EFIATLFMTIFMVKKKIFRS"
     gene            8031..9122
                     /gene="glf"
     CDS             8031..9122
                     /gene="glf"
                     /note="OgN12_7"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="BAU71622.1"
                     /translation="MKYDFLIVGAGLFGATCAHELTKKGKKVLILEKRNHIAGNIYTE
                     NVNGIHVHKYGAHIFHTNDKNIWDYVNSFVEFNRFTNSPLAFYKGKIYNLPFNMNTFY
                     QLWGTKTPEEARKKIAEQKMQVEGIVPSNLEEQAISLVGRDIYECLIKGYTEKQWGRK
                     ATELPDFIIKRLPVRFTYDNNYFSDKFQGIPVGGYTKLVEKMIEGIDIKLNTDFLKNR
                     EYYLSQAKHVIYTGPIDEYYSYRFGQLDYRSLKFENELLEMENFQGNAVVNYTEREIP
                     YTRIIEHKHFDPVETPYTVITKEYPQEWKEGDEPYYPINDAKNMELFKKYRMLAKDEN
                     IIFGGRLAEYKYYDMHQVIRSALNTVEAL"
     CDS             9125..10114
                     /note="OgN12_8"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="hypothetical protein"
                     /protein_id="BAU71623.1"
                     /translation="MQKYNKVVVCCPAELVTGGPELLHQFVHHLTEQGVDAHITYYPF
                     CSKHSTPEAYKHYKINIISLSEIEASEDILIVIPETATQISKRFPYNSIAIWWLSVDN
                     YFGYPLSENNIYKKGVHWLKILLGKKLSLRKMREMIHFYQSEYARLFLQHNNITALSL
                     KDYLNKSHMDIGYANVEKENVILYNPKKGIAITNKLKENFREFQFKPIENMTASEVRI
                     TLERSKLYIDFGHHPGKDRFPREAVMAGCVLITGVEGSAANEVDIPIPSRFKLDSNKF
                     NFESEFKKCVDNIFSNFQNEIKEFEYYRNCIKNEEQDFRNDVTSFVRSYIKQK"
     CDS             10735..11313
                     /note="OgN12_9; transposase of ISAs1 family"
                     /pseudogene="unitary"
                     /gene="orf10"
                     /codon_start=1
                     /transl_table=11
     CDS             11365..12261
                     /note="OgN12_10"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /product="glycosyltranslocase"
                     /protein_id="BAU71624.1"
                     /translation="MMKHQAKIYAVIVTFNPDIESLRKNLCSISEQVDRVVIIDNSND
                     LVCQNRLQVMISENENITLIQLNENKGIAYAQNIGFVYSLKAHADFVITFDQDSSVEN
                     GLVQSLYEEYLKVKDENDIKIACIGPSVINDRNKQEYENYFHNSVKINESVYSVNFII
                     SSGTLYETDAFVAIGLNKAEWFIDSIDIEWCYRARYLGYQVLMTTKATMKHNLGSRDI
                     QLPLGRLFNIGAPFRHYYVYRNWIFSLREPHFDYGYKFKLVFMMPIKFIICAAIPPRK
                     KRILFMLKGIRDGILKRHSFIN"
     gene            12575..13636
                     /gene="wzy"
     CDS             12575..13636
                     /gene="wzy"
                     /note="OgN12_11"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAU71625.1"
                     /translation="MFLIEFTFILLSLCIIFQVNTKPSLIYTIMLLFIALFMAGIAGF
                     RIDSPDYENYYLYFNMLSKGIDYRQINIVAPDPAFALLNICLSRLSTNPLILFLFFGI
                     TSVLINAFCFKKYVKYFMISMLFYLVHTYVARELMQIRAGLACALCLFSLRYIVNKCP
                     WRFLITIILASSFHLGAVVFLIAYPLGQYKFNSKKVAIAIICALIISSIFPLGAFFKS
                     LPSYAFLNRIQYYNDTEYGQSSGLFTNVVIIKELLIIIVCLAYRRVLENIPYFNVSFN
                     TYVVSLIWLILWNDFSIVASRIATFYSIGEVLLMAMLPFITKSGGSRNILAVLLILLA
                     GVIMFMNIYTGKWDGVVII"
     CDS             13640..14740
                     /note="OgN12_12"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf13"
                     /product="glycosyltranslocase"
                     /protein_id="BAU71626.1"
                     /translation="MYMNILHVTNTISEGGVESFLLQLLPQLNRHHKCSLFVLNKNKI
                     DMASMFMNNGVPVIHNKYKSIYNPLVVLHLFGIAKNYDVIHSHLFPTQYYTAVLKLLF
                     LFTNKKIKFVTTEHCTTNKRRRPLFKLIEKLVYSSYNKIIGVSDAATQNLKKWIDISD
                     NKVVTIHNGIDIRSILSASSYSKATFGIPEEQFVLCMTARFFEQKDHSSLIKAMQFLP
                     DNIVLLLIGSGNKFEESIKLCETLKLQNRIKFLGRRRDVYSIIKLADICILSTHYEGF
                     PVSILEYFCAGKPVIATNVDGVNEMLDDSSLLCNPNDPHDLACKILNLSMDNNKMNKE
                     SERNRMIIEKYSLENMTENYLQIYECLFEQLK"
     CDS             14737..15645
                     /note="OgN12_13"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf14"
                     /product="glycosyltranslocase"
                     /protein_id="BAU71627.1"
                     /translation="MKKVIAIIVTFKRKHLLCKVIESILNQTVKPMEIIIIDNNSNDG
                     TREVVKKFFNNGRVNISYYNTGANLGGAGGFKIGIQKALERKSEYIWLMDDDLSPDEH
                     CLEYLLDSVNSSTICQPMRLNVDSTCAEYSPTCYDLKSVFTLRPKRETVINFFNSKKD
                     AQIDLHGIPFEGPLIPTPIVSQIGLPDERFFIFYDDLDYALRARKIGCKIKCITKAKA
                     TRLLKNNQVNDLSSWKGYFMLRNFFRIHLIYGENIFVRSRPLLITIMYILKSILTGNV
                     KAARICFDAYIDHKSKSFRTSRRYIP"
     gene            15941..17347
                     /gene="gnd"
     CDS             15941..17347
                     /gene="gnd"
                     /note="OgN12_14"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAU71628.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGRKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFNKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            17596..18762
                     /gene="ugd"
     CDS             17596..18762
                     /gene="ugd"
                     /note="OgN12_15"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAU71629.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKENSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            18872..19885
                     /gene="wzz"
     CDS             18872..19885
                     /gene="wzz"
                     /note="OgN12_16"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAU71630.1"
                     /translation="MRTWKFPSVRVMMRVENNNVSGQNHDPEQIDLIDLLVQLWRGKM
                     TIIISVIVAIALAIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSD
                     LQETLIGRFSSAFSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKL
                     AQYIQQVDDKVNQELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQY
                     ANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDI
                     ESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNY
                     NAK"
     gene            complement(19981..20592)
                     /gene="hisI"
     CDS             complement(19981..20592)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAU71631.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGEMSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAARKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     6326 a   3868 c   4537 g   6289 t
ORIGIN
        1 atcgcatcct cggcattggc ccgtgggaac gacgcctgcg taccctcatc gaacaatatc
       61 aactggaaga tgtggtagag atgcctggct ttaaaccgag ccatgaagtg aaagcgatgc
      121 tcgacgacgc ggatgtcttc ctgttgccat cggtaacggg cgcggatggc gatatggaag
      181 gcattccggt ggcgctgatg gaagcgatgg cggtcggtat tccggtggtt tctactctgc
      241 atagcggaat accggaactg gtggaggccg ataaatccgg ctggctggtg cctgagaacg
      301 atgctcgcgc actggcacaa cgtctggcgg cgtttagcca actggacacc gacgaattag
      361 ctccggtcgt caaatgcgcg cgcgaaaaag ttgaacacga ttttaaccag caggtgatca
      421 atcgagaact cgccagcttg ttacaggctt tatagaggag aacgtatgcc atttaaaaaa
      481 ctctcccgac gcaccttcct gacggcaagc tcggcgcttg ccttcctcca tacccctttc
      541 gctcgcgcgc ttcccgcccg acaaagcgtt aacattaacg actacaaccc acacgactgg
      601 atcgcctcat ttaaacaagc cttcagcgaa gggcaaacgg tcgtcgtgcc tgccggattc
      661 gtttgtgaca atatcaacac cggcatcttc attcctcctg acaaaacgtt acatatcctt
      721 ggaagcctgc gcggcaacgg cagagggcga tttgtcttac aggacggcag ccaggtgaca
      781 ggggaggagg gcggcagtat gcataacatc accctggatg tgcgtggatc tgactgcacc
      841 atcaaagggc tggcgatgag cggctttggt ccggtaacgc agatttatat cggcggcaaa
      901 aacaaacggg tcatgcgcaa cctgaccatc gataatctca ccgttaacca cgctaattac
      961 gctattttgc gccagggatt tcataaccag attatcggtg ccaacatcac caactgtaaa
     1021 tttagtgatt tacagggcga cgccattgaa tggaacgtgg cgattaacga cagtgatatt
     1081 ttgatatctg accatgtcat cgagcgcatc aactgtacca acggcaaaat caactgggga
     1141 atcggcatag gccttgcagg aagcacttac gataataatt acccggaaga ccaggcagtg
     1201 aaaaactttg tcgtggcgaa tatcacggga tcggattgtc ggcagttgat ccatgttgaa
     1261 aatggtaaac attttgttat tcgaaatatc aaagcccgca atatcacacc ggatttcagt
     1321 aagaaagcag gtattgataa cgccacagtc gctatttacg gttgtgacaa tttcgtgatt
     1381 gataatattg aaatgattaa tagcgccggg atgttaatcg gctatggggt aattaaaggc
     1441 aaatatctct caataccgca aaatttccga gtgaataata ttcaaatgga taacacccat
     1501 cttgcttata aattgcgcgg catccaaatc tccgccggga atgctgtctc ctttgtggcg
     1561 ctgactaaca ttgagatgaa gcgtgcgtca ttggagttac acaacaagcc gcaacatctt
     1621 tttatgcgta atatcaaggt gatgcaggaa tcctcagttg gacccgcatt gagcatgaac
     1681 ttcgacatgc gcaaagacgt tcgcggcgtg tttatggcga aaaaagaaac actgctgtct
     1741 cttgcaaatg ttcatgcggt gaatgaaaga gggcaaagct ccgtcgatat cgacaggata
     1801 aatcaccatg ttgttaatgt ggaaaagatt aactttagat tgccggaacg gagggagtag
     1861 atttgcgacc attcctggaa aaatggagtc atacttagga acaatgctac tgcaatccac
     1921 aacgaagcgg cgtaacatca caagtaattc agtaatcaat tcagggtaat tgatgctggc
     1981 gaaaaaaatc gaacaagcta taattcagca accattttac aggtggatga aataatgacg
     2041 aatttaaaag cagttattcc tgtagcgggt ctagggatgc atatgttgcc tgccactaag
     2101 gcgattccca aagagatgct accgatcgtc gacaagccaa tgattcagta cattgttgac
     2161 gagattgtgg cggcagggat caaagaaatc ctcctggtaa ctcacgcgtc caagaacgcg
     2221 gtcgaaaacc acttcgacac ctcttatgag ttagaatcac tccttgagca gcgcgtgaag
     2281 cgtcaactac tggcggaagt gcaatctatc tgcccgccgg gcgtgaccat tatgaacgtg
     2341 cgtcagggcg aacctttagg tttaggccac tccattttgt gtgcacgacc tgccattggt
     2401 gacaacccat ttgtcgtggt actgccagac gttgtgatcg acgatgccag cgccgacccg
     2461 ctacgttaca accttgctgc catgattgcg cgcttcaacg aaacgggccg cagccaggtg
     2521 ctggcaaaac gtatgccggg cgatctctct gaatactccg tcattcagac caaagaacca
     2581 ctggatcgtg aaggtaaagt cagtcgcatt gttgaattca tcgaaaaacc ggatcagccg
     2641 cagacgctgg actcagacat catggccgtt ggtcgctatg tgctttctgc cgatatttgg
     2701 ccggaactgg aacgcactca gcctggtgca tgggggcgta ttcagctgac tgatgccatt
     2761 gccgaactgg cgaaaaaaca gtccgttgac gccatgttga tgactggaga caactacgac
     2821 tgcggtaaaa aaatgggtta tatgcaggcg tttgtgaagt atggactacg caacctgaaa
     2881 gaaggggcga agttccgtaa aagcattgag aagctgttaa gcgaataatg aaaatctgac
     2941 cgggtgtaac ggttgataag aaaattataa cggcagtgaa gattcgtggc gaaagtaatt
     3001 tgttgcgaat tttcctgccg ttgctttata taaacaatca gaataacaaa gagttagcaa
     3061 taggattttc gtcaaagttt tccaggattt tccttgtttc cagagcggat tggtaagaca
     3121 attagcgttt gaatttttcg ggtttagcgc gagtgggtaa cgctcgtcac atcgtaggca
     3181 tgcatgcagt gctctggtag ctgtaaagcc aggggcggta gcgtgcatta ttacctctat
     3241 taatcaacct gagagcagct tatttcacag catgctctga agtaatatgg aataaattaa
     3301 gtgaaaatac ttgttactgg tggagcagga tttattggtt ctgctgtagt tcgtcacatt
     3361 ataaataata cgcaggatag tgttgttaat gtcgataaat taacgtacgc cggaaacctg
     3421 gaatcacttg ctgatgtttc tgattctgaa cgctatattt ttgaacatgc ggatatttgc
     3481 gatgctgctg caatggcacg gatttttgct cagcatcagc cgcatgcagt gatgcacttg
     3541 gctgctgaaa gccatgttga ccgttcaatt acaggtcctg cggcatttat tgaaaccaat
     3601 attgttggta cttatgtcct tttggaagcc gctcgcaatt actggtctgc tcttgatagc
     3661 gacaagaaaa atagcttccg ttttcatcat atttctactg acgaagtcta tggtgatttg
     3721 cctcatccag atgaagtaaa taataacgaa gaattaccct tatttactga gacaacagct
     3781 tacgcgccaa gcagccctta ttccgcatca aaagcatcca gcgatcattt agtccgagcg
     3841 tggaaacgta cctatggttt accgaccatt gtgactaatt gctctaacaa ttatggtcct
     3901 tatcattttc cggaaaaatt gattccattg gttattctca atgctctgga aggtaaagca
     3961 ttacctattt atggtaaagg ggatcaagtt cgcgattggc tgtatgttga agatcatgct
     4021 cgagcgttat atacagtcgt tactgaaggt aaagcaggcg aaacctataa tattggcgga
     4081 cacaacgaaa agaaaaacat cgatgtagtg cttactattt gtgatttgct ggatgagatt
     4141 gtaccgaaag agaaatctta tcgtgagcaa attacttatg ttgccgaccg cccgggacac
     4201 gatcgccgtt atgcgattga tgctgagaag attagccgcg aattgggctg gaaaccgcag
     4261 gaaacgtttg agagcgggat tcggaagaca gtggaatggt acctgtccaa tacaaaatgg
     4321 gttgataatg tgaaaagtgg tgcctatcaa tcgtggattg aacagaacta tgagggccgc
     4381 cagtaatgaa tatcctcctt tttggcaaaa cagggcaggt aggttgggaa ctacagcgtg
     4441 ctctggcacc tctgggtaat ttgattgctc ttgatgttca ctccactgat tactgtggtg
     4501 attttagtaa tcctgaaggt gtagctgaaa ccgtaagaag cattcggcct gatattattg
     4561 tcaacgcagc cgctcacacc gcagtagaca aagcagaatc agaaccgaag tttgcacaat
     4621 tacttaacgc gacgagtgtc gaagcgatcg cgaaagcagc caatgaagtc ggcgcttggg
     4681 ttattcacta ctctactgac tacgtatttc cggggaccgg tgaaatacca tggctggaga
     4741 cggatgcaac cgcaccacta aatgtttacg gtgaaaccaa gttagccgga gaaaaggcgt
     4801 tacaggaata ttgcgcgaag catcttattt tccggaccag ctgggtctat gcaggaaaag
     4861 gaaataactt cgccaaaacg atgttacgtc tggcaaaaga gcgtgaagaa ttagcggtta
     4921 ttaacgatca gtttggtgcg ccaacaggtg ctgaactgct ggctgattgt acagcacatg
     4981 ccattcgtgt cgcactgaat aaaccggatg tcgcaggctt gtaccatttg gtagccagtg
     5041 gtaccacaac ctggtacgat tatgctgcgc tggtttttga agaggcgcgc aaagcaggca
     5101 ttccccttgc actcaacaag ctcaacgcag taccaacaac cgcctatcct acaccagctc
     5161 gtcgtccaca taactctcgc cttaatacag aaaaatttca gcagaacttt gcgcttgtat
     5221 tgcctgactg gcaggttggc gtgaaacgaa tgcttaacga attatttacg actatagcaa
     5281 tttaatagtt tttgcatctt gttcgtgatg gtggagcaag atgaattaaa aggaatgatg
     5341 aaatgaaaac gcgtaaaggt attattttag cgggtggttc tggtactcgt ctttatcctg
     5401 tgactatggc agtcagtaaa cagctgttac ctatttatga taaaccgatg atctattacc
     5461 cgctttctac actgatgtta gcgggtattc gcgatattct gattatcagt acaccacagg
     5521 atactcctcg ttttcaacaa ctgctgggtg acgggagcca gtgggggcta aatcttcagt
     5581 acaaagtaca accgagtcca gatggtcttg cgcaggcatt tatcatcggt gaagagttta
     5641 tcggtggtga tgattgtgct ttggttctag gtgataatat cttttacggt catgatctgc
     5701 cgaagttaat ggatgtcgct gttaacaaag aaagtggtgc aacggtattt gcctatcacg
     5761 ttaatgatcc tgaacgctac ggtgttgttg agtttgataa aaacggtacg gcaatcagcc
     5821 ttgaagaaaa accgctacaa ccaaaaagta attatgcggt aaccgggctt tatttctatg
     5881 ataacgacgt tgtcgaaatg gcgaaaaacc ttaagccttc tgcccgtggt gaactggaaa
     5941 ttaccgacat taaccgtatt tatatggaac aggggcgttt atccgttgcc atgatggggc
     6001 gtggttatgc atggctggat acggggacac atcagagtct tattgaagca agcaacttca
     6061 ttgccacaat tgaagagcgc cagggactaa aggtttcctg cccagaagaa attgcttacc
     6121 gtaaagggtt tattgatgca gagcaggtga aagtattagc tgaaccgctc aagaaaaatg
     6181 cttacggcca atacctgtta aaaatgatta aaggttaatt aataaaatga acataattaa
     6241 aactgaaatt caagacgtgt tgatattcga gcctgaagta tatgaagatg aacgtggctt
     6301 ttttatggaa agttttaacc aaagaatatt ggaaaaaaac atatgtaaaa atgtaaaatt
     6361 tgttcaggat aatcactcca aatccatgca aggtgttttg agaggattgc actatcaatt
     6421 atctcctttt gctcaaggga aattagttag atgcaccgta ggccgggtat ttgatgttgc
     6481 ggttgatctt agaaggacat ctgaaacatt tggtcattat gtatcctttg aattgtcggc
     6541 agaaaataaa aaaatattgt ggattcctga aggctttgct cacggatttt tagttttaag
     6601 cgatgtggct gaattccaat ataaaacaac taattattat agtccaaaac atgagcgtag
     6661 cataatatgg aatgatccta tattaaatat tcattggccg attttaaatg aaagtgattt
     6721 gataatttca caaaaggatg ctggtgctat tttatttaat aacgcagaat attttgatta
     6781 gagtgttacg tggataaaaa actattaaag aatatttttt cattactttg tgtacaaggt
     6841 gtaagttatt taattccttt aataacttta ccgtatttag ttaaaacatt ggggcctcat
     6901 tcctatggtg tgctagggtt ctcattagca atttggcagt attttataat gctgactgat
     6961 tatggtttta atttaactgc aactcaaaga atagcacaat atcccgatga taagtataag
     7021 gttagtttag tgttttggga ggttatgata tgtaagttaa tattaatgct cgtgtgcggg
     7081 attgtaataa ttatattgtt acggtttacc agtattttaa gtggttacga gagtgtattt
     7141 tttgctggtg gaggcttact tttaggaaac attttatatc caatttggct ctttcaggga
     7201 aaagaatcaa tggcgttatc ctccatcgta aatgttacaa caagattgtc gacaattcca
     7261 ttaacctttt tattagttaa ttcagttgat gatgcttggg tgtcagcttt aattaattca
     7321 acaacctcta ttctgggggg gggggtcgca cttttgatcg tttaccataa aaaatggtta
     7381 attctggtta ccccccacaa aaaaggatta aagaaacaat ttctggaagg ttaccatgta
     7441 tttatgtcta ctgctgcgat aaatttatat accacaagcg ttacagtaat tcttggaatc
     7501 ataagcggtc ccattgttgt aggttatttt gttgctgcgg ataagttaag acaggctgtt
     7561 caaggaatta tcgttccatt ttcccaagcc tgttatccaa ggattgtttc cttgatgcaa
     7621 aaaaatagaa aggatggact tcaatttata cgaaagatgc ttatcctaca gggaggggga
     7681 gggggtattc taagcatgtt tctctttgta atgactcctg atataatcaa tttaatgtat
     7741 ggggacgaat attaccgctc ggttattaca ctacaaatat tgtcattatg ccctgttttg
     7801 gtagcaatta gtaatgtatt aggcattcag acaatgctta ctttgggtta taaaagagag
     7861 ttttttttaa tattattaat atctagtttg ttttgtatca ctttacttta tccgttgtgt
     7921 atccacttga tggagaatgg agccgctcta tcggttgttt tgtcagagtt tatcgctact
     7981 ctttttatga ctattttcat ggttaagaaa aaaatattta ggagttaaga atgaaatatg
     8041 attttttaat agttggggcg gggctttttg gcgctacttg tgctcatgag ctcacaaaaa
     8101 aaggaaaaaa ggtactaatt ttagaaaaaa gaaatcatat agcaggaaat atttatacgg
     8161 aaaacgtgaa tggcatacat gtccataaat atggtgcgca catatttcat acaaatgata
     8221 aaaacatatg ggactatgtc aatagtttcg ttgagtttaa tcgttttaca aactcaccat
     8281 tagcttttta taagggtaaa atttataatt taccttttaa tatgaataca ttttatcaat
     8341 tatggggaac aaaaactcca gaagaggctc gtaaaaaaat agccgagcag aaaatgcaag
     8401 ttgaaggcat cgtaccatca aatttggagg agcaagcaat atctttggtt ggtcgagata
     8461 tctacgaatg cttgataaaa ggttataccg aaaaacaatg gggaagaaaa gcaacagaac
     8521 ttccagattt tattattaaa cgacttcctg ttagatttac atacgataat aattatttct
     8581 cagataaatt ccaaggtata cctgtgggcg gatatacaaa acttgtagaa aaaatgatag
     8641 aaggtattga tataaaatta aatactgatt tccttaaaaa cagagagtat tatctctctc
     8701 aagctaagca tgttatatac acagggccta ttgacgaata ttacagttat aggtttggtc
     8761 aattagatta tcgatcatta aaatttgaaa atgaattgtt ggagatggaa aattttcaag
     8821 ggaatgcagt ggttaattac actgaacgtg aaatacccta taccagaatt attgaacaca
     8881 aacactttga tcctgtcgaa actccttata cggtaataac aaaagaatat cctcaggagt
     8941 ggaaagaagg ggatgaacca tattatccaa taaatgatgc taaaaatatg gagctattca
     9001 aaaaatatcg tatgttggca aaagatgaaa atataatttt tggggggagg ctcgctgaat
     9061 ataaatatta tgatatgcat caagtcattc gctctgcgct taacacagtg gaagcattat
     9121 aaatatgcaa aaatataata aagtggttgt ttgttgtccg gcagaacttg tgactggagg
     9181 accagaatta cttcatcagt ttgtgcatca cctcactgag caaggtgtgg atgcgcatat
     9241 aacatactat ccattttgct ctaagcattc aactcccgaa gcttataaac attataaaat
     9301 taacatcata tcgttatcag aaattgaagc aagtgaggat atattaatag taatacctga
     9361 aacagctacc caaataagta aaagatttcc ttataactcg attgccattt ggtggctatc
     9421 agtagataat tatttcggat atccgttatc tgaaaataat atctataaaa aaggagtgca
     9481 ctggttaaaa atattactag gaaaaaaact ctctctaaga aaaatgaggg aaatgattca
     9541 tttttatcaa agtgaatatg ccagactgtt tcttcaacat aataatataa ccgctctttc
     9601 tttgaaggat tatttaaata aaagtcatat ggatataggt tacgcgaacg ttgaaaaaga
     9661 gaatgtcata ttatataatc caaaaaaagg tattgctatt actaataaat tgaaagaaaa
     9721 tttcagagag tttcaattta aaccgataga gaatatgaca gcctctgaag tccgtataac
     9781 gcttgagcgc tctaaattgt atattgattt tgggcatcat cccggaaaag atcgtttccc
     9841 tagggaagca gtaatggctg gttgtgtctt aattactggt gttgaaggta gtgctgctaa
     9901 tgaagtagat ataccaatac cgagtcgttt taaattggat agtaataagt ttaattttga
     9961 gagcgaattt aaaaaatgcg ttgataatat tttttctaat ttccaaaatg aaataaaaga
    10021 gttcgaatat tatcgaaact gtataaaaaa cgaggagcaa gatttcagga atgatgtgac
    10081 ttcatttgtc agatcatata tcaaacaaaa atgaaactaa gcgatgcttt gcatcgctta
    10141 aatagcagtt aaatatctat tcattcatat ggctttatag aattacagct atcaattaaa
    10201 tctcgactgg agtatgttaa tattgaattg tttagaaaat aatatgtaat tatcgtgata
    10261 atgtgggatc ttctaaattg ctgagtctaa cacccctaca cttttgccag cccatttttc
    10321 cctcccgggg atggactgat gctttccata aaatgacgaa cttttctcaa cagctgccag
    10381 atcgtcctgc atggatgatt gcgggttatc gtatcgtgaa gtgactgcca cagatgctca
    10441 accagggaaa gattatcaga agtacacttt ttgtactaga taatttgcat tttatgttta
    10501 aaaattgaga tattcctcat tacctaaagc tgttttttat tgcttataca tgatcaaata
    10561 ctccttacat aattaaggag aacaaaatgg tacttaaaaa attgatggaa catatttcta
    10621 ttatccccga ttacagacaa gcctggaaag tggaaaataa attatcggat attctactgt
    10681 tgactatttg cgccgttatt tcgggtgcag aaggttagga agatatagag aattttgggg
    10741 aaacacatct cgattttttt gaagcaatat ggtgattttg aaaatggtat tcctgttcac
    10801 gataccattg ccagagttgt atcctgtttc agtcctgcaa aatttcacga gtgctttatt
    10861 agctggatgc gtgactgcca ttcttcagat gataaaggcg tcattgcaat tgatggaaaa
    10921 acgctccggc attcttatga caagagtcgc cgcaggggag cgattcatgt cattagtgcg
    10981 ttctcaacaa tgcacagtct ggtcatcgga cagatcaaga cggatgagaa atccaatgag
    11041 attacagcta tcccagaact tcttaacatg ctggatatta aaggaaaaat tatcacaact
    11101 gatgcgatgg gttgccagaa agatattgca gagaagatac aaaaacaggg aggtgattat
    11161 ttattcgcta taaaaggaaa ccaggggcga ctaaataaag ccctcgagga aaaacttccg
    11221 ctgaaagaat taaataatcc agagcatgac agttacgcaa ttagtgaaaa gagtcacggc
    11281 agagaagaaa tccgtcttca tttgatattt taaaccattt agattaatag tcttatcaag
    11341 atgtattttt agaaggtaaa ataattgatg aaacatcaag ctaaaatata tgcagtaatt
    11401 gttactttta atccagatat tgaatcgttg agaaaaaatt tatgttctat ttctgaacag
    11461 gttgatagag ttgtaatcat tgataacagt aatgatctcg tttgtcagaa ccgtttacag
    11521 gtaatgataa gtgaaaatga aaacatcact ctaatccagc ttaatgaaaa caaaggtatt
    11581 gcttatgcac aaaatatcgg ttttgtctat tcgttaaagg cgcatgccga ttttgttata
    11641 acgtttgatc aggattcatc tgttgaaaat gggttagttc aatcattata tgaagagtat
    11701 ttaaaagtaa aagatgagaa cgatataaaa attgcatgca ttggtccttc agttatcaac
    11761 gataggaata agcaggaata tgaaaattat tttcataata gcgtcaaaat taatgaatct
    11821 gtgtatagtg ttaacttcat aatttcttcc ggaactttat atgaaaccga tgcttttgta
    11881 gcgattggcc ttaacaaggc cgaatggttt attgactcaa ttgatattga gtggtgctat
    11941 agagctcgct atctaggata tcaggtatta atgacgacga aagcaactat gaaacataac
    12001 cttggatcga gagatattca gcttccgtta ggaaggcttt ttaatatagg tgctcctttc
    12061 cggcactact atgtatacag aaattggata ttttcattgc gagaaccaca ttttgattat
    12121 ggatataagt ttaaacttgt gttcatgatg cctataaagt ttataatttg cgcagccatt
    12181 cccccacgaa agaaaagaat tttgttcatg ttaaaaggaa ttcgagatgg aattcttaaa
    12241 agacattcct ttatcaacta atagcaatta gcaatatgat tgttgtattt ttgtgatgag
    12301 aatatccaca tgatttttaa taagataatt tttaatatat aaaatgtttt ttataatgtt
    12361 ttagagggga tgctaaagta ttgctttata aaagtgctat tttattcgat gtgaaatgct
    12421 tttgatggaa atactttttg tgtttcagtt atttttagtg gggatggagt ttgttagcat
    12481 ttttgtattt accgaacaaa tatcattcat taacattaat gcataaaagt agcacttatt
    12541 tcttaaattt ttacaataac atgagtgatg aataatgttt ctcattgagt ttacttttat
    12601 attgttgtcc ctttgtataa tttttcaggt taatactaag ccctcattaa tatatactat
    12661 tatgttattg tttatagctt tattcatggc tggtattgca ggcttcagaa tagattcgcc
    12721 tgattatgaa aattattatt tatattttaa tatgttatct aagggaatag attaccggca
    12781 aattaatatt gtggcacctg atcctgcttt tgctttatta aatatatgtt tatcaaggct
    12841 ttctacaaat cctttgattt tatttttatt ttttggcatt acatcagtct tgataaatgc
    12901 tttttgtttt aaaaaatatg ttaaatactt tatgataagt atgctattct atttggtgca
    12961 tacttatgtg gcccgcgaac taatgcaaat aagagcaggg ttagcatgtg ctctatgttt
    13021 attttcattg cggtacatag ttaataaatg tccatggagg tttttaataa caataatatt
    13081 agctagttcc tttcatctag gggcggttgt ttttcttata gcttatccgt taggccaata
    13141 taaatttaac tctaaaaagg tcgctattgc aataatatgt gcattgatta ttagtagtat
    13201 tttcccattg ggtgcctttt tcaaaagtct acctagttac gcttttctga atagaattca
    13261 gtattataat gatactgaat acggccaaag tagtggttta tttaccaatg ttgttattat
    13321 taaagagttg ttgataatta tagtttgcct tgcttatcgt agagttttgg aaaacatccc
    13381 ttattttaat gtgtctttta atacttatgt agtatcatta atatggttaa tattatggaa
    13441 tgatttttca attgtagcct cacggattgc aacattttat tcaataggtg aggttttatt
    13501 aatggctatg cttcctttta taacaaaatc aggtgggtcc agaaatattc ttgctgtgct
    13561 tttaatctta cttgccggtg ttatcatgtt tatgaatata tacacaggaa aatgggatgg
    13621 tgttgttatt atttaagagg tgtatatgaa tattctacat gttacgaata caatttctga
    13681 agggggagta gaatcctttc ttctacaatt attgccacaa ctaaatcgtc atcacaaatg
    13741 ttccttattt gttctaaata aaaataaaat agacatggca tcaatgttta tgaataatgg
    13801 agttcctgtt attcataata aatataaaag catttataat cctcttgttg tattacattt
    13861 gtttgggatt gcgaaaaatt atgatgtaat acacagccat ttatttccaa cacagtatta
    13921 tactgcggtg cttaaactat tattcttatt tacaaataaa aaaataaaat ttgtcactac
    13981 agagcattgc actactaaca aaagaagaag gcctttattt aaattaattg aaaagcttgt
    14041 ttacagttca tataataaaa taataggtgt tagtgatgct gctactcaaa atttaaagaa
    14101 atggattgat attagtgata acaaggtcgt aactattcat aatggtattg atattcgatc
    14161 tattttatct gcttcttctt atagtaaagc aacgtttggt attcccgaag aacaatttgt
    14221 gctatgtatg acagcacgtt tttttgagca aaaagaccat tcaagcctta taaaagcaat
    14281 gcaattttta ccagataata tcgttttgct tttaattggt tccggcaata agttcgagga
    14341 gtccattaaa ctttgtgaga ctctaaaatt acaaaacaga ataaagtttt tagggagaag
    14401 acgagatgtt tattcaataa taaaattagc tgacatatgt attttgtcaa ctcattacga
    14461 aggatttcct gtttcaattt tggagtattt ttgtgcagga aaaccagtaa ttgcaactaa
    14521 tgtagatggt gttaacgaaa tgttagatga ttcttcatta ttgtgcaatc ctaatgatcc
    14581 tcatgatttg gcttgtaaaa tattaaattt aagcatggat aataataaaa tgaataagga
    14641 atcagaaaga aaccggatga taatagagaa atattctcta gagaatatga cggaaaatta
    14701 tcttcaaatc tatgaatgtc ttttcgagca acttaaatga aaaaagttat tgcaattatc
    14761 gtaacattta aacgtaagca cctgctgtgt aaggttatag agtctatatt aaatcaaaca
    14821 gtcaagccaa tggagataat tattatagac aataatagta atgatggtac gcgagaagta
    14881 gttaaaaaat tttttaataa tggtagagtc aatatttctt attataacac tggtgcaaat
    14941 ttgggaggag caggaggatt taaaattggt attcagaagg cacttgaaag gaaatcagaa
    15001 tatatctggc ttatggatga tgatttaagc cctgatgaac attgtttaga atacttactt
    15061 gacagtgtaa atagtagcac aatttgccaa ccaatgagac taaatgtaga tagtacttgt
    15121 gctgaatatt caccaacatg ttatgatcta aagtctgtat ttaccctaag gcccaagcgt
    15181 gaaactgtca ttaatttttt taattctaaa aaagatgcgc agattgattt gcatgggata
    15241 ccattcgaag gtccattaat ccccactcca atagtttcac aaataggttt gcctgatgaa
    15301 agatttttta ttttctatga tgatcttgat tatgcattaa gagccagaaa gatcggatgt
    15361 aaaattaagt gtataactaa agcaaaagca actagattat tgaaaaacaa tcaagttaat
    15421 gatttatctt catggaaagg atacttcatg ttaagaaact tctttagaat tcaccttatt
    15481 tatggagaaa atatttttgt tagatccaga ccattgctta taactattat gtatatattg
    15541 aaatcaatat taactggaaa tgtaaaagcc gctaggatat gttttgatgc ttatatagat
    15601 cataaaagca aatcatttag aacttcaagg cgatatattc cctgaattaa tgcaattata
    15661 attcatcgga cttgttttac tttgtaatgg gtaattttaa ttagctactg agttatattc
    15721 acatgagggt ttaatcctga ttaactacta atgaattatg atttgataag tcgatattat
    15781 tttgctgagt aaactataat tatcacacca taggcgttct taagtattat gcatttttct
    15841 atctagaatg gtgtttctgc ccacacaaag attataattt acgggattat aaaactgtat
    15901 attttccaaa cggtgatcca ccccctgaca ggagtaaaca atgtcaaagc aacagatcgg
    15961 agtagtcggt atggcagtga tggggcgcaa ccttgcgctc aacatcgaaa gccgtggtta
    16021 taccgtctct attttcaacc gttcccgtga aaagacggaa gaagtgattg ccgaaaatcc
    16081 aggcaggaaa ctggttcctt actatacggt gaaagaattc gttgaatctc ttgaaacgcc
    16141 tcgtcgcatc ctgttaatgg tgaaagcagg tgcaggcacg gatgctgcta ttgattccct
    16201 gaaaccatat ctcgataaag gcgatatcat cattgatggt ggtaatacct tcttccagga
    16261 caccattcgt cgtaaccgtg agctttctgc agaaggcttt aacttcatcg gtacgggtgt
    16321 ttccggtggt gaagagggcg cgctgaaagg tccttccatt atgcctggtg gccagaaaga
    16381 agcctatgaa ctggttgctc cgatcctgac caaaatcgcc gcagtagctg aagacggtga
    16441 gccatgcgtt acctatattg gtgccgatgg cgcaggtcac tatgtgaaga tggttcacaa
    16501 cggtattgaa tacggtgata tgcagctgat tgctgaagcc tattctctgc ttaaaggtgg
    16561 cctgaatctt tccaacgaag aactggcgca gacctttacc gagtggaata acggtgaact
    16621 gagcagctac ctgatcgaca tcaccaaaga tatcttcaac aaaaaagatg aagacggtaa
    16681 ctacctggtt gatgtgattc tggatgaagc agcaaacaaa ggtacgggca aatggaccag
    16741 ccagagcgcg ctggatctcg gcgaaccgct gtcgctgatt accgagtctg tgtttgcacg
    16801 ttatatctcg tctctgaaag atcagcgtgt tgccgcgtct aaagttctct ctggtccgca
    16861 agcgcagcca gcaggcgaca aggctgagtt tatcgagaaa gttcgtcgtg cgctgtatct
    16921 gggcaaaatc gtttcttatg ctcagggctt ctctcagctg cgtgcggcgt ctgaagagta
    16981 caactgggat ctgaactacg gcgaaatcgc gaagattttc cgtgctggct gcattatccg
    17041 tgcgcagttc ctgcagaaaa tcaccgatgc ttatgccgaa aatccgcaga tcgctaacct
    17101 gctgctggct ccgtacttca agcaaattgc cgatgactac cagcaggcgc tgcgtgatgt
    17161 cgttgcttat gcagtacaga acggtatccc ggttcctacc ttcgccgctg cggttgctta
    17221 ttatgatagc taccgtgccg ctgttttgcc tgcgaacctg atccaggcac agcgtgacta
    17281 tttcggtgcg catacttata agcgcattga taaagaaggt gtgttccata ctgaatggct
    17341 ggattaatct gatttaacca acaataaaat tgatgcccgg cgtatattgc accgggcttt
    17401 tttttgccaa atatcttata gactaaattt cactgcttaa tattaactta ataaatatca
    17461 gctattctta taaagaaaat ctgaattgtt tttcgttgcg ttgaccatct gacaacgtag
    17521 cgttaaaact tttagctctt atcaggatgc taaaaacatc atgattcaca gttaagttaa
    17581 ttctgagagc atgaaatgaa aatcaccatt tccggtactg gctatgtagg cttatcaaac
    17641 gggcttctaa tcgcacaaaa tcatgaggtt gtggcattag atattttacc gtcacgcgtt
    17701 gctatgctga atgatcggat atctcctatt gtcgataagg aaattcagca gtttttgcaa
    17761 tcagataaaa tacactttaa tgccacatta gataaaaatg aagcctaccg ggatgctgat
    17821 tatgtcatca tcgccactcc aaccgactat gatcctaaaa ctaattattt caatacatcc
    17881 agtgtagaat cagtaattaa agacgtagtt gagataaacc cttatgcggt tatggtaatc
    17941 aaatcaacgg ttcccgttgg ttttaccgca gcgatgcata aaaaatatcg tactgaaaat
    18001 attatattct ccccggaatt tctccgtgag ggtaaagccc tttacgataa ccttcatccg
    18061 tcacgtattg tcatcggtga gcgttcagaa cgcgcagaac gttttgctgc gttgttacag
    18121 gaaggcgcga ttaagcaaaa tatcccgacc ctgtttaccg actccactga agcagaagcg
    18181 attaaacttt tcgctaatac ctatctggcg atgcgcgtgg cgtactttaa cgaactggat
    18241 agctatgcag aaagtttggg tctgaatacg cgtcagatta tcgaaggtgt ttgtctcgac
    18301 ccgcgtatcg gcaaccatta caacaatccg tcgtttggtt atggtggtta ttgtttgccg
    18361 aaagatacca aacagttact ggcgaattat cagtctgtgc cgaataacct gatttcggca
    18421 attgtcgatg ctaaccgcac gcgtaaagat tttattgccg atgccatttt gtcacgcaag
    18481 ccgcaagtgg tgggtattta tcgtctgatt atgaagagcg gttcagacaa cttccgcgcg
    18541 tcttccattc aggggattat gaaacgtatc aaggcgaaag gcgttgaagt catcatttac
    18601 gaaccggtga tgaaagaaaa ctcattcttc aactctcgcc tggaacgaga tctcgccact
    18661 ttcaaacaac aagctgacgt cattatctcc aatcgtatgg cagaagagct taaggatgtg
    18721 gcagataagg tatacacccg cgatctcttt ggcagcgact aacatcttgt tatcagggct
    18781 atttacgccc tgattgtctt ttgttacttc ctcaacaatt ctttattttt atcacttatc
    18841 ctatagcatt cacgaggatt atcgctaaac tatgcggact tggaaatttc cgtcagttag
    18901 ggtaatgatg agagtagaaa ataataatgt ttctgggcaa aaccatgacc cggaacagat
    18961 tgatttgatt gatttactag tgcagttgtg gcgtggcaag atgacaatca tcatttccgt
    19021 cattgtggct attgccctgg ctattggata tttggcagta gcgaaggaga aatggacgtc
    19081 aacagcaatt atcactcagc ccgacgtggg gcaaattgct ggctataaca atgccatgaa
    19141 tgttatctat ggtcaggctg caccgaaagt atcggatttg caggagacgt taattggtcg
    19201 cttcagttct gccttctctg cattagcaga aacgctggat aatcaggaag aaccagaaaa
    19261 acttaccatc gaaccttctg ttaagaacca gcaattacca ttaactgttt cttatgttgg
    19321 gcaaactgca gagggcgcac aaatgaagtt ggcccaatac attcagcaag ttgacgataa
    19381 agtgaatcaa gagttagaaa aggatctcaa ggacaacatt gctctgggac ggaaaaactt
    19441 gcaggactct ttaagaacgc aggaagtagt cgcgcaggag cagaaagatc tgcgtatccg
    19501 tcagattcag gaagcgttgc agtatgcgaa tcaggcgcag gtgacaaagc cacaggttca
    19561 gcagactgaa gatgtgacac aagatacgtt gttccttttg gggagcgaag cgctggagtc
    19621 gatgattaag catgaggcga cccgtccgtt ggtattctca ccaaactact atcagacacg
    19681 tcaaaacctg ctggatatcg aaagcttaaa agttgatgat cttgatattc atgcttaccg
    19741 ctatgtaatg aaaccgacgt tacctattcg tcgcgatagt ccgaaaaagg caatcacctt
    19801 gattctggcg gtgctgctgg gcggcatggt tggcgcgggg attgtgctgg ggcgtaacgc
    19861 tctgcgtaat tacaacgcga agtaatcttt tcggttttaa agaaaatggg cagggtggtg
    19921 acaccttgcc cgtttttttg ccggatgcga caacaacatc gcatccgctt accccgcaac
    19981 tcactgatgc cgtttacgca ggttctcaat taccgtcgtt aaatccagat cctgatcctg
    20041 caacagcacc agcaggtgat acatcaaatc agatgcctcg ttagtcagct caaagcggtc
    20101 atgtaccgtc gctgccagtg cggtttccac gccttcttca cctactttct gcgcaatgcg
    20161 tttggtgccg ctggcataca gtttggcggt gtaggaggtt tccgggtcgg cagatttgcg
    20221 tgcggcgagc agttgttcca gttgatacag gaacagccat tggtgagcgg tgtcgccgaa
    20281 gcagctgctg gtgcctttgt ggcaggttgg tccgatagga ttcgccagta ccagtagcgt
    20341 gtcgttgtcg caatccgggg caatactcac tacgttgagg aaattgcccg acatttcgcc
    20401 tttggtccac agccgttgtt tagtgcgcga gaagaaggtg actttgccgc tttcgatggt
    20461 tttgtctaaa gcttccgggt tcatatagcc cagcattagc acttcgccgg ataccgcgtg
    20521 ttgcacaatt accggcatca gtccgtcggt tttttcccag tccagttcgc gacgttgttg
    20581 ttctgttaac atatcctgat ctccacgccc tgtgttgcca ggtacgcttt taattcacca
    20641 atattgatta tttgtttgtg gaatacggaa gctgccagcg cgccgtcaac gtcggcatcg
    20701 cggaaggctt cgaggaagtg ttccatggtc cccgcgccac cggaggcaat cagtgggacg
    20761 tggcaaactt cacgcacttt tttcagttgt tcgaggtcgt aaccgttacg cacgccgtcc
    20821 tgattcatca tgttgaggac gatttctccg gcaccgcgtt tttgcacttc ctctacccag
    20881 tcgagcgttt cccactgagt gacgcgggtg cggctttcat cgccggtata ttgattcaca
    20941 tgatatttcc cggtttcgcc gtcgtaccag gtatcaatac cgaccacaat acactgcacg
    21001 ccaaaacgat ccgccaggcg
//
