LOCUS       LC177552               13654 bp    DNA     linear   BCT 15-DEC-2018
DEFINITION  Escherichia coli DNA, O-antigen biosynthesis gene cluster OgN15,
            strain: E819.
ACCESSION   LC177552
VERSION     LC177552.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., von Mentzer,A., Kikuchi,T. and Thomson,N.R.
  TITLE     An untypeable enterotoxigenic Escherichia coli represents one of
            the dominant types causing human disease
  JOURNAL   Microb Genom 3 (2017) In press
  REMARK    DOI:10.1099/mgen.0.000121
            Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13654)
  AUTHORS   Iguchi,A., von Mentzer,A. and Thomson,N.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2016) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuenkibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..13654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E819"
                     /db_xref="taxon:562"
                     /note="Escherichia coli O-antigen biosynthesis gene
                     cluster OgN15"
     gene            14..1408
                     /gene="wcaM"
     CDS             14..1408
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAV90509.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFGEGQTVVVPAGLVCDNINTGIFIPLGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            1571..2476
                     /gene="galF"
     CDS             1571..2476
                     /gene="galF"
                     /note="OgN15_1"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAV90510.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSA"
     gene            2967..4208
                     /gene="wzy"
     CDS             2967..4208
                     /gene="wzy"
                     /note="OgN15_2"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAV90511.1"
                     /translation="MLTLLVLFVLFIALVGRKYNRYGIYYSCFIFFYVVCHYFYFLTK
                     DGFYHIELDSDEGLSFLQTTILLILIIITYRTLSSIFKIREPILNGVIIGSGYVLLLM
                     LYFLGVLSYVLQNGIALGLSYNDRLTANTGGGLSIILMYAYIPALILIYISKPSKISL
                     IICLLLSVFCGLIYYVVIGGSRNVLAAGIFSLIYLALYFKHITKKFLALIIVCGVFTL
                     MILELYRYANNITDAINFIMNGGMQVILFAFESFSPMHAVININEALDKRLIEPQYLS
                     TFFNEFSIIIPRFLWEDKPINVLNNGYFYTTEILSLDTNLTMSPTFLGTSLIMFGSWF
                     YWVGGFISGVILFIFDRSFSHSSNLYWKIILLSSVGYLFFWVRDGFEVFCYILIKFFI
                     VMFIYKNLTIIYKSLARKNEF"
     gene            4198..5385
                     /gene="wzx"
     CDS             4198..5385
                     /gene="wzx"
                     /note="OgN15_3"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAV90512.1"
                     /translation="MNFKQKGKIISYILKLSVLAFTVLPGANRVVLFYIISHVTTTEY
                     FAFFSSAYSYAIVMSMIGGVGVGTVIIKYGSEFGIVNFIKIAFIAIIISSIAGGIIIF
                     SFWEVTRLGIIGLLLMSIGLTMNQIIRYMIIVKKNFLIGAINEVIFILPPFVFLFIKD
                     INIIILLGSLYIIQSLLTAFFCFVDNGKTIKFREAFIIGHSNLVSSGILYFLPIASLS
                     LVGPVITKAVSLMITTAGIITVFPRAMLNMKIVDIQSLYNNDRGEFLKESARFKNRVA
                     CVMLLGVIIMIAYGCLTNKTSSIVDIIYIGLSLSLFIFMGQYTILETTLINLVGKENI
                     SLILNSLSFIFFVGVYYILKIIDLQNTTLTMFLLCSIVFISYSIRYYIINKVLNEVGK
                     CLL"
     CDS             5373..6863
                     /note="OgN15_4"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf5"
                     /product="hypothetical protein"
                     /protein_id="BAV90513.1"
                     /translation="MSFVTINTELPSELNHVLSKNKKLITDVIFGNSVSIYFDPNDWH
                     HYPVIKSEKSSIYVSGWFINENYERNNIVWLKEVLDSKNNISDVQKRIIAGVFICIYE
                     TKGEMSVFTDPFALSPHYYYIKDNKLSLSPSPCVFTNEIDHELNDILNAQGHLFGKYT
                     IYKNVYRTLPGDVITCTDGKLGVYENGYEVMNTELPFDVINEAKKLILTTHKSMQSIA
                     ISAGFDSRLLLIVSEPEFAYTWGPNGSADVRNGKTLAAHKKIPYHSFGFRVNKVTESD
                     ERICELLFKGSVKSYNTQFFANYNYVHNLSKSNTIALDGYLGDVLQRGVYMTFGGKKG
                     EFYKLFPSITSSFVDAKMLLRKRYRKLNAKQFDYVYADFLKKVSKVECIDSLQKITYY
                     EFLYGRGLRYITTGAIVMNSCFKSVFPVFASRNIFNYLIKQKANDVLTYKVFYDIWKD
                     ENAFYRTMKSEGAYSPSMKPIFIPFVNLLGRIITNFHPKYKNYTKE"
     CDS             6866..7915
                     /note="OgN15_5"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf6"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90514.1"
                     /translation="MKILLLSNMYPSEKEPSFGIFVETIANQLSDIGYDVELCVRKRK
                     CNGRISKLLSYMFFYIRAFLYLNFKKYDYIYCHYVSHISLPVICAIFLGRRLRVISHV
                     HGGDVKHLNGRSFIFHKIKNVFTVTLFKHSLSILCPSQQYAKYLCENYSVNISKVIVY
                     PSGGVKKCFKYETSFPVHDESVKIGFAGRLVKSKNVDLIINAIKQLKNVQLSIVGDGE
                     QKDYLNKLAKDCDIEFLGPMDHNELARWYKTINVLVYPSESESLGLVPLEAMSSGVYC
                     ILSKIPAFYEIRQHGLTFSFIENYDSQSIAREIENFQKINVVYLNKILKRNSDIVNEV
                     YGESNVKRILINVFK"
     CDS             7902..8387
                     /note="OgN15_6"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90515.1"
                     /translation="MSLNKKHILFVYGEGGHAAQMNRLAPIILNNLEDFEAITLSDST
                     KYPHWSCWHNKTIEFRDKYSWLSLLKNIGPLIIFKQTYSILRKKNIKVIVTTGPGIAI
                     AATLAAKLVGTKVIYIETWSRFTTYSLSGRIMYRLSNKFYIQNKSLLKLYPKAIYSGR
                     L"
     CDS             8384..8845
                     /note="OgN15_7"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90516.1"
                     /translation="MRIFVTVGTTSFDSMIENFDHYFNENKKYNVNFQIANGNYIPKS
                     GAFFSFRDDVDVFYNDADVIITHAGAGTLYQLLEKGKKIIAVPNLERIDKHQVDIATY
                     MERNHYLLVCWDYNEIGDLLKSIHNFKPNPYYKIEFDKGKEIADYIRSLYF"
     gene            8966..10372
                     /gene="gnd"
     CDS             8966..10372
                     /gene="gnd"
                     /note="OgN15_8"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAV90517.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLNKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            10594..11787
                     /gene="ugd"
     CDS             10594..11787
                     /gene="ugd"
                     /note="OgN15_9"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAV90518.1"
                     /translation="MFNRKKSELMKITISGTGYVGLSNGILIAQNHEVVALDIVQAKV
                     DMLNKKQSPIVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFN
                     TSSVEAVIRDVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYD
                     NLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVA
                     YFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQS
                     VPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRI
                     KAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRD
                     LFGSD"
     gene            11877..12911
                     /gene="wzz"
     CDS             11877..12911
                     /gene="wzz"
                     /note="OgN15_10"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAV90519.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETA
                     EGAQKQLAQYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVAKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(13008..13619)
                     /gene="hisI"
     CDS             complement(13008..13619)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAV90520.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELKNEASDLVYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4058 a   2513 c   2892 g   4191 t
ORIGIN
        1 tagaggagaa cgcatgccat ttaaaaaact ctcccgacgc accttcctga cggcgagctc
       61 cgcgcttgcc ttccttcata cgcctttcgc ccgcgcactt cccgcccgac aaagcgttaa
      121 cattaacgac tacaacccac acgactggat cgcctcattt aaacaagcct tcggcgaagg
      181 gcaaacggtc gtcgtgcctg ccggattggt ttgcgacaat atcaacaccg gcatcttcat
      241 tcctcttggc aaaacgttac acatccttgg aagcctgcgc ggcaacggca gagggcgatt
      301 tgtcttacag gacggtagcc aggtgacagg ggaggagggc ggcagtatgc ataacatcac
      361 cctggatgtg cgtggctctg actgcaccat caaagggctg gcgatgagcg gctttggccc
      421 ggtaacgcag atttatatcg gcggcaaaaa caaacgggtc atgcgcaacc tgaccatcga
      481 taacctcact gtcagccacg ctaattacgc catcttacgc cagggatttc ataaccagat
      541 aatcggtgcc aacatcacca attgtaagtt cagcgactta cagggcgatg ccattgaatg
      601 gaacgtggcg attaacgacc gtgatatttt gatctccgac catgtcatcg agcgcatcaa
      661 ctgtactaac ggcaaaatca actggggcat cggcataggc cttgcgggga gtacttatga
      721 taataactac ccggaagacc aggcagtgaa aaactttgtc gtggcgaata tcacgggatc
      781 ggattgtcgg cagttgatcc atgttgaaaa tggtaaacat tttgttattc gtaatatcaa
      841 agcccgcaat atcacgccgg atttcagtaa gaaagcaggt attgataacg ccacagtcgc
      901 tatttacggt tgtgacaatt tcgtgattga taatattgaa atgattaata gcgccggaat
      961 gttaatcggc tatggggtaa ttaaaggcaa atatctctcg ataccgcaaa atttccgagt
     1021 gaataatatt caactggata atacccacct tgcttacaaa ttgcgcggta tccaaatctc
     1081 cgctgggaat gccgtctcct ttgtggcgct gactaacatt gagatgaagc gtgcgtcgct
     1141 ggagttacac aacaaaccgc aacatctttt tatgcgtaat atcaaggtga tgcaggaatc
     1201 ctcagttgga cccgcattga gcatgaactt tgacatgcgc aaagacgttc gcggcgtctt
     1261 tatggcgaaa aaagaaacac tgctgtctct tgcaaatgtt catgcggtga atgagaaagg
     1321 acaaagctcc gtcgatatcg acaggataaa tcaccatatt gttaatgtgg aaaagattaa
     1381 ctttagattg ccggaacgga gagagtagat ttgcgaccat tcctggaaaa atggagccat
     1441 acttaggaac aatgctactg caatccacaa cgaagcggcg taacatcaca agtaattcag
     1501 taatcaattc agggtaattg atgctggcga aaaaaatcga acaagctata attcagcaac
     1561 cattttacag gtggatgaaa taatgacgaa tttaaaagca gttattcctg tagcgggtct
     1621 tgggatgcat atgttgcctg ccactaaggc gattcccaaa gagatgctac cgatcgtcga
     1681 caagccaatg attcagtaca ttgttgacga gattgtggct gcagggatca aagaaatcct
     1741 cctggtaact cacgcgtcca agaacgcggt cgaaaaccac ttcgacacct cttatgaatt
     1801 agaatctctt cttgaactgc gcgtgaagcg tcaactactg gcggaagttc agtccatctg
     1861 cccgccgggc gtgactatta tgaacgtgcg tcagggcgaa cctttaggtt taggccactc
     1921 cattttgtgt gcgcgacctg ccattggtga caacccattt gtcgtggtac tgccagacgt
     1981 tgttatcgac gacgccagcg ccgacccgct gcgttacaac cttgcagcca tgattgcgcg
     2041 cttcaacgaa acaggccgca gccaggtgct ggcaaaacgt atgccgggcg atctctctga
     2101 atactccgtt attcagacca aagagccgct ggaccgcgaa ggtaaagtca gccgcatcgt
     2161 cgaatttatc gaaaaaccgg atcagccgca gacgctggac tcagacatca tggccgttgg
     2221 ccgttatgtg ctttctgccg atatttggcc ggaacttgaa cgcactcagc caggtgcatg
     2281 gggacgtatc caactgacag atgccattgc cgaactggcg aaaaaacagt ctgttgatgc
     2341 aatgctgatg actggtgaca gctacgactg cggtaaaaaa atgggctata tgcaggcgtt
     2401 cgtgaagtat gggctgcgca acctcaaaga aggggcgaag ttccgtaaag gcattgagaa
     2461 gctgttaagc gcataatgaa aatctgaccg aatgtaacgg ttgataagaa aattataacg
     2521 gcagtgaaga ttcgtggcga aagtaatttg ttgcgaattt tcctgccgtt gttttatata
     2581 aacaatcaga ataacaacga gttagcaata ggattttagt caaagttttc caggattttc
     2641 cttgtttcca gagcggattg gtaagacaat tagcgtttga atttttcggg ttaagcgcga
     2701 gtgggtaacg ctcgtcacat cgtaggcatg catgcagtgc tctggtagct gtaaagccag
     2761 gggcggtagc gtatgttata aataatgata ttttcgatgt gagcaattat aaggctccta
     2821 actctactta agatattaaa tatcaattat atgtgttggc ttattaatat gagaagtctt
     2881 cattgattaa gatttatggt gttataataa ttgagaagcc atagaaatgc tattttaatt
     2941 gagtgcgatt actttgagag taaacaatgc taactttact ggttttattc gtattattta
     3001 ttgcgttagt aggcaggaaa tataacaggt atgggattta ttactcttgt tttatttttt
     3061 tctatgtggt ctgccattat ttttattttt taactaaaga tggattttat catattgagc
     3121 tggattcaga tgaggggctt tctttcctgc aaacaacaat tttgctaata ttaattatta
     3181 ttacttatag gacattgtcc tctattttta aaatacgtga acctatatta aatggcgtga
     3241 taattggttc tgggtatgtt ttattactga tgttatattt cttaggtgtt ttatcttacg
     3301 tactacaaaa tggaattgca ctaggattat catataatga ccgattaaca gccaacacag
     3361 gtggtggatt atctataata ttaatgtatg catacatacc agcattaatc cttatttata
     3421 tatcaaaacc ctctaagatc agcttaatta tatgtttatt attaagtgtg ttttgcggtt
     3481 tgatttatta tgttgtgata ggtggttcca gaaatgttct agcagctggt atattttcac
     3541 ttatctactt ggctctatat ttcaaacata tcacaaagaa gtttctggca ttgattatcg
     3601 tttgtggtgt gttcacatta atgatattag aactatatcg ttatgcaaat aatattactg
     3661 atgctataaa ttttattatg aatggaggaa tgcaagtaat attatttgca ttcgaatctt
     3721 tttcaccaat gcatgctgtg ataaatatta atgaagcact agataagaga ttaattgaac
     3781 ctcaatacct gtcaactttt tttaatgagt tttctattat catcccaagg tttctatggg
     3841 aagataaacc gataaatgtt ttaaacaatg gatattttta tactactgaa attctatctt
     3901 tagacactaa tttaactatg tcgccaactt ttttaggaac atccttaata atgtttggta
     3961 gttggttcta ttgggtaggt ggattcatta gtggggttat attgtttatt tttgatagat
     4021 cattttctca ctcatccaat ctctattgga aaataatatt actaagtagt gttgggtact
     4081 tattcttttg ggtcagagat ggatttgaag tattttgcta tattttgata aagtttttta
     4141 tagtcatgtt tatttataaa aatcttacaa taatttataa gtcattggcg aggaaaaatg
     4201 aattttaaac agaaaggaaa aataatctca tatattttga aattatctgt tttggcattt
     4261 acggtattac cgggggctaa tagggtagta cttttttata taatatcaca tgtaacaaca
     4321 acagagtatt ttgctttttt ttcctctgcg tattcttatg ctatagtaat gtctatgatt
     4381 ggtggggtgg gggtcggaac tgtaataatc aagtatggtt ctgaattcgg aattgttaat
     4441 ttcattaaaa ttgcattcat agctattatt ataagtagta ttgctggagg gattatcatt
     4501 ttttcttttt gggaagttac aaggttaggc ataatagggt tgttattaat gtcaattggt
     4561 ttgacaatga atcaaatcat acgttatatg ataatagtta aaaaaaattt tttgattggc
     4621 gcaattaatg aagtgatttt tattcttccc ccatttgttt ttttatttat caaagatata
     4681 aatataatta tactgcttgg ttctttatat attattcaat ctttacttac agcatttttc
     4741 tgttttgttg ataatggaaa aactataaaa tttagagagg cttttattat tggtcattca
     4801 aatttagttt cttcaggtat actttatttt cttcctatcg ccagtttatc actagttggt
     4861 ccggtaataa ctaaagcagt ttcactaatg atcacaaccg ctggaattat tacggtattc
     4921 ccgcgtgcta tgttgaatat gaaaattgta gatattcaga gtttatataa taatgaccga
     4981 ggcgaatttt taaaagaatc tgctaggttt aaaaatcggg tggcatgtgt aatgctgtta
     5041 ggtgttatta ttatgattgc atatggatgt ttgacaaata aaacatcatc tatagtagat
     5101 atcatttata ttggtttgag tttatctctt tttatattta tggggcagta tactattctt
     5161 gaaacaacac taattaattt ggttgggaaa gaaaacattt cgttaatatt gaattcatta
     5221 agtttcatct tttttgttgg agtttactac atattaaaga taatagactt gcaaaatacg
     5281 acactaacta tgtttttatt atgctctatt gtttttatat cgtattcgat tcgatattat
     5341 attattaata aggttttaaa tgaggttgga aaatgtcttt tgtaacaata aatacagaac
     5401 taccaagtga attaaatcat gtattaagca agaataaaaa acttattacc gacgtaatat
     5461 ttggtaattc ggttagtatt tatttcgatc caaacgattg gcatcattat cctgtaatca
     5521 aatctgaaaa aagcagtatc tatgtttctg gttggtttat aaatgaaaat tatgaaagga
     5581 ataatatagt atggctgaaa gaagttctgg attcaaaaaa taatattagc gatgtacaaa
     5641 agcgtattat tgctggggtg tttatttgta tctatgaaac aaaaggggaa atgtctgttt
     5701 ttacagatcc atttgcactt tcacctcatt attattatat aaaagataat aaactatctt
     5761 tatccccatc accatgtgta tttacaaatg agattgatca tgagcttaat gatattttaa
     5821 acgctcaagg tcatttgttt ggtaaatata caatatataa gaatgtttat cgcacattac
     5881 caggtgatgt tattacttgt actgatggta agttgggtgt ttacgaaaat ggttatgaag
     5941 tcatgaatac tgaattacca tttgatgtta ttaatgaagc caaaaagtta attttaacaa
     6001 cacataaatc gatgcaatcc attgcaatat ctgcaggatt tgattctcgc ttactattaa
     6061 ttgtatcaga accagaattt gcttacacat gggggccaaa tggttctgca gatgtaagaa
     6121 acgggaagac tcttgcagcg cataaaaaaa taccttatca ctcatttggt tttcgagtta
     6181 ataaagtaac ggaaagtgat gagaggatat gtgaattatt attcaaagga agcgtaaagt
     6241 cttataacac acaatttttt gctaactata attacgtaca taatttatcc aaaagtaata
     6301 caatagcgtt agatggatac cttggagatg tgctacagcg tggcgtctat atgacttttg
     6361 gtggtaaaaa aggagaattt tataaattat ttccatcaat tacatcatct tttgtcgatg
     6421 cgaaaatgtt actgaggaaa cgatatagaa aactcaatgc caaacaattt gattatgtat
     6481 atgctgattt tttaaaaaag gtttctaaag tagaatgtat tgattcattg caaaaaataa
     6541 cgtactacga gtttttatat ggtcgaggtc ttcgatatat tactacaggg gctatagtaa
     6601 tgaattcatg ttttaagagt gttttcccag tttttgcatc gagaaatata tttaattatc
     6661 taattaaaca aaaagctaat gatgttttaa cgtacaaagt tttctatgac atatggaaag
     6721 atgagaatgc attttataga acaatgaaat ctgagggtgc atacagtcca tctatgaaac
     6781 caatattcat tccatttgtt aatttattag ggcgaattat tactaacttt catccgaaat
     6841 acaaaaatta tacaaaggag taaggatgaa aattttatta ttgagcaata tgtatccttc
     6901 cgaaaaggaa ccgagttttg gaatatttgt tgaaacaatt gcaaatcagt tatctgatat
     6961 tggctatgat gttgagctct gtgtcaggaa aaggaaatgc aatggccgaa tatctaaact
     7021 cttaagttat atgttttttt atatacgagc gttcctttat ttgaacttca aaaaatatga
     7081 ctatatatat tgccattatg tttctcatat atcgcttcct gttatatgtg ccattttttt
     7141 aggcagaaga ttacgtgtaa tcagtcatgt gcatggtggg gatgtaaaac acctcaacgg
     7201 aagaagtttt atattccata agataaaaaa tgtatttacc gtaacattgt ttaaacatag
     7261 cttatcaata ttatgtccct ctcaacaata tgctaaatat ttatgtgaaa actatagtgt
     7321 taatataagt aaagttattg tttatccttc tggtggtgtt aaaaaatgtt tcaaatatga
     7381 aacctccttt cctgtacatg atgaatctgt aaaaattgga tttgcaggta gattggttaa
     7441 atcaaaaaac gtagatctta taataaatgc cattaaacaa ttaaaaaacg tacaattaag
     7501 cattgtcggt gatggtgagc aaaaagatta tcttaataaa ttagctaagg attgcgatat
     7561 tgaatttcta ggtcccatgg atcataatga attagcaagg tggtataaaa ccattaatgt
     7621 gttagtttat ccttccgaaa gtgaaagttt gggtttagta ccattagaag ctatgtcatc
     7681 tggtgtttat tgtatattat caaaaatccc agcattctat gaaattagac aacatggttt
     7741 aacattctct tttattgaaa actatgactc tcaatctata gctagagaaa ttgaaaattt
     7801 tcaaaaaatt aatgttgtct atttaaataa aatattaaaa agaaatagcg atatagttaa
     7861 tgaagtttac ggtgagtcaa atgttaaaag gatattaata aatgtcttta aataaaaaac
     7921 atatattatt tgtttatggt gaaggtgggc atgctgctca aatgaatcga cttgctccta
     7981 taatacttaa caacctcgag gattttgaag caataacttt atctgactct acaaagtacc
     8041 ctcattggtc ctgctggcat aataaaacta tagaatttag agataaatat agttggttat
     8101 ctttattgaa aaacataggt cccctaataa ttttcaaaca aacatattct attttacgca
     8161 aaaagaatat taaggttatc gttacaactg gaccaggaat tgcgattgca gcaacgctgg
     8221 cagcgaaatt agttggcaca aaagtaattt atattgaaac ttggtcgaga tttacaacat
     8281 actctttatc cggaagaata atgtatcgat tgtctaataa attttatatt caaaataagt
     8341 cactcttaaa attatatcct aaagcaattt attcaggtag gttatgagaa tttttgttac
     8401 tgtagggacg acctcatttg attctatgat tgaaaatttc gatcattact ttaatgagaa
     8461 taaaaagtac aatgttaatt ttcaaatcgc aaatggtaat tatataccca aaagtggtgc
     8521 ttttttttct tttagggatg atgttgacgt tttctataat gacgcggatg taataattac
     8581 ccatgccggg gctgggacac tatatcagct cttagaaaaa gggaagaaaa ttatagcggt
     8641 tcctaatctt gaacgtatag ataaacacca agtcgatata gctacttata tggagcgtaa
     8701 tcattatttg ttagtttgtt gggattacaa tgagataggt gatttgttaa aatctattca
     8761 taattttaaa cctaatccgt attacaagat tgagttcgac aaaggcaagg aaatagctga
     8821 ttatataagg tcattgtact tttagtttaa tgtattgtgt tattttattt tttaaagaaa
     8881 atttcgagta tatagcttaa tcccatttaa cagaattcat cgcataactg cccctcgtgg
     8941 ctaatttccc tgacaggagt aaacaatgtc aaagcaacag atcggcgtcg tcggtatggc
     9001 agtgatgggg cgcaaccttg cgctcaacat cgaaagccgt ggttataccg tctctatttt
     9061 caaccgttcc cgtgaaaaga ccgaagaagt gattgccgaa aatccaggca agaaactggt
     9121 tccttactat acggtgaaag agttcgttga atctctggaa acgcctcgtc gcatcctgtt
     9181 aatggtgaaa gcaggtgcag gcacggatgc tgctattgat tctctcaagc catacctcaa
     9241 taaaggtgac atcatcattg atggtggtaa caccttcttc caggacacca tccgtcgtaa
     9301 ccgtgagctt tctgcagaag gctttaactt catcggtacc ggtgtctccg gtggtgagga
     9361 aggcgcacta aaaggtcctt ccattatgcc tggtggccag aaagaagcct atgaactggt
     9421 tgcaccgatc ctgaccaaaa tcgccgcagt ggctgaagac ggtgagccat gcgttaccta
     9481 tattggtgcc gatggtgcag gtcactatgt gaagatggtt cacaacggta ttgaatacgg
     9541 tgatatgcag ctgattgctg aagcctattc tctgctaaaa ggtggcctga atctttccaa
     9601 cgaagaactg gcgcagacgt ttaccgagtg gaataacggt gaactgagca gctacctgat
     9661 cgacatcacc aaagatatct tcaccaaaaa agatgaagac ggtaactacc tggttgatgt
     9721 gattctggat gaagcggcta acaaaggtac cggtaaatgg accagccaga gtgcgctgga
     9781 tctcggcgaa ccgctgtcgc tgattaccga gtctgtgttt gcacgttata tctcttctct
     9841 gaaagatcag cgtgttgccg catctaaagt actgtctggt ccgcaagcgc agccagcagg
     9901 cgataaggct gagtttatcg agaaagttcg tcgtgcgctg tatctgggca aaatcgtttc
     9961 ttacgcccag ggcttctctc agctgcgtgc tgcgtctgaa gaatacaact gggatctgaa
    10021 ctacggcgaa atcgcgaaga ttttccgtgc tggctgcatc atccgtgcgc agttcctgca
    10081 gaaaatcacc gatgcctatg ccgaaaatcc gcagatcgct aacctgctgc tggctccgta
    10141 cttcaagcaa atcgccgatg actatcagca ggcgcttcgc gatgtcgttg cttatgcagt
    10201 acagaacggt atccctgttc ctaccttcgc tgctgcggtt gcctattacg acagctaccg
    10261 cgccgctgtt ctgcctgcga acctgatcca ggcacagcgt gactatttcg gtgcgcatac
    10321 ttataagcgc attgataaag aaggtgtgtt ccataccgaa tggctggatt aatctgattt
    10381 aaatcaatta atcaaagcaa ggcccggaga aaccctccgg gcttttttat tatacaaagc
    10441 ggcaggttat ggcctttttt tataatttat ggttaaaaaa gcgatataat acagagccgc
    10501 acagcaggat cgctgccttg acagttcatc tacatcagcg ttaaaaatcc cgcagtagat
    10561 gaagctgtgg tggtggatta atgaccactc taaatgttta accggaagaa gtcagagcta
    10621 atgaaaataa caatttcagg aacaggttat gttggtcttt caaatggtat tctgattgcg
    10681 caaaaccacg aagtggttgc actggatatc gttcaggcca aagtggacat gcttaacaag
    10741 aagcagtcac cgattgttga taaggagatt gaagagtatc tggcgactaa agatctcaat
    10801 ttccgcgcta cgacagataa gtatgacgcg tataaaaatg ccgattacgt tattattgcc
    10861 acacctaccg attatgatcc gaaaacgaat tattttaata cctcaagcgt ggaagcggtc
    10921 attcgcgatg tgacagaaat taatcctaat gctgtcatgg tgattaaatc tacaattccg
    10981 gtaggtttca ccaaatcaat taaagaacgt ctgggtatcg ataaccttat tttctctccg
    11041 gaatttctcc gtgaaggtaa agccctttac gataatctcc atccgtcacg tattgtcatc
    11101 ggtgagcgtt cagaacgtgc agaacgtttt gctgcgttgt tacaggaagg cgcgattaag
    11161 caaaatatcc cgaccctgtt taccgattca accgaagcag aagcgattaa acttttcgct
    11221 aatacctatc tggcgatgcg cgtagcgtac tttaatgaac tggatagcta tgcagaaagt
    11281 ttagggctga atactcgcca gattatcgaa ggcgtttgtc tcgatccgcg tattggcaac
    11341 cattacaaca acccgtcgtt tggctatggt ggttattgtc tgccgaaaga taccaagcag
    11401 ttactggcga actaccagtc tgtgccgaat aacctgattt cggcaattgt cgatgcaaac
    11461 cgcacgcgta aagattttat tgccgatgcc attttgtcac gcaagccgca agtggtgggt
    11521 atttatcgtc tgattatgaa gagcggttca gataacttcc gcgcgtcttc cattcagggg
    11581 attatgaagc gtatcaaggc gaaaggcgtt gaagtgatca tatacgagcc agtgatgaaa
    11641 gaagactcat tcttcaactc tcgcctggaa cgagatctcg ccaccttcaa acaacaagcc
    11701 gacgtcatta tttccaaccg tatggcagaa gagcttaagg atgtggcaga caaagtctac
    11761 acccgcgatc tctttggcag tgactaacat cctgttatca gggcgatttt cgccctgatt
    11821 ctcttatgtt ccctttgtaa taattcatta tttttatcat ttatcctata gcattcatgg
    11881 cgattatcgc taaactatgg cgacttaaaa ttatttcgtc cgttagggta atgatgagag
    11941 tagaaaataa taatgtttct gggcaaaacc atgacccgga acagattgat ttgattgatt
    12001 tactggtgca gttgtggcgc ggcaagatga caattatcat ctctgtcatt gttgctattg
    12061 ccctggctat agggtatctg gccgttgcca aagaaaagtg gacttccaca gctatcgtta
    12121 ctcaacctga tgtggggcaa atcgctggtt ataccaacgc gatgaatgtg atttacggat
    12181 cggctgtacc gaaagtctcc gacattcagg cttcgcttat cggacgttac agcactgcat
    12241 tctcagcatt agcggaaacg ctggataacc aggaagaagc agaaaagctg accattgagc
    12301 ctaccgttaa aaatcagtca ttacctttag cggtttctta tgttggtgaa acagctgaag
    12361 gtgcgcagaa acagctggcg caatatatcc agcaagttga cgaccaggta aacgaagaac
    12421 tggaaaaaga cctgaaagac aacatcgcgc tgcgtatgaa aaacttgcag gattctttga
    12481 agactcagga agtagttgcg caggagcaga aagatctgcg tatccgtcag attcaggaag
    12541 cgttgcagta tgcgaatcag gcgcaggtgg caaagccaca ggttcagcag actgaagatg
    12601 tgacacaaga tacgttgttc cttttgggga gcgaagcgct ggagtcgatg attaagcatg
    12661 aagcgactcg tccgttggtg ttctcaccaa actactatca gacacgtcaa aacctgctgg
    12721 atatcgacaa tcttgacgtt gataaacttg atattcatgc ttaccgctat gtaatgaaac
    12781 cgacgttacc tattcgtcgc gatagcccga aaaaggcaat caccttgatt ctggcagtgc
    12841 tgctgggcgg tatggtgggc gcggggattg tgttggggcg taacgctctg cgtaattaca
    12901 acgcgaagta atcttttcgg ttttaaagaa aaagggcagg gtggtgacac cttgcccgtt
    12961 ttttttgccg gatgcgatgt tgttgtcgca tccgcttacc ccgcaactca ctgatgccgt
    13021 ttgcgcaggt tctcaatcac cgttgttaaa tccagatcct gatcctgcaa cagcaccaac
    13081 aggtgataca ccaagtcaga tgcctcattc ttcagctcaa agcggtcatg tactgtcgct
    13141 gccagtgcgg tttccacgcc ttcttcgccc actttctgcg caatgcgttt ggtgccgctg
    13201 gcatacagtt tggcggtata ggaggtttcc ggatcggcag atttacgttc ggcgagcaat
    13261 tgttccagtt gatacaggaa cagccactgg tgagcggtat cgccgaagca gctgctggtg
    13321 cctttgtggc aggtcgggcc gatgggattc gccagcacca gtaacgtgtc gttgtcgcag
    13381 tccggcgcaa tacttactac gttgaggaaa ttgcccgacg tttcgccttt ggtccacagt
    13441 cgctgtttag tgcgcgagaa gaaggtgact ttgccgcttt cgatggtttt gtctaaggct
    13501 tccgggttca tgtagcccag cattagcact tcgccggata ccgcgtgttg cacaatcacc
    13561 ggcatcagtc cgtcggtttt ttcccagtcc agttcgcgac gttgttgttc tgttaacata
    13621 tcctgatctc cacgccctgt gttgccaggt acgc
//
