LOCUS       LC177553               19127 bp    DNA     linear   BCT 15-DEC-2018
DEFINITION  Escherichia coli DNA, O-antigen biosynthesis gene cluster OgN16,
            strain: E1642.
ACCESSION   LC177553
VERSION     LC177553.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., von Mentzer,A., Kikuchi,T. and Thomson,N.R.
  TITLE     An untypeable enterotoxigenic Escherichia coli represents one of
            the dominant types causing human disease
  JOURNAL   Microb Genom 3 (2017) In press
  REMARK    DOI:10.1099/mgen.0.000121
            Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19127)
  AUTHORS   Iguchi,A., von Mentzer,A. and Thomson,N.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2016) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuenkibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..19127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E1642"
                     /db_xref="taxon:562"
                     /note="Escherichia coli O-antigen biosynthesis gene
                     cluster OgN16"
     gene            14..1408
                     /gene="wcaM"
     CDS             14..1408
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAV90521.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEVHTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEKGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLAIDNLTV
                     SHANYAILRQGFHNQIIGASITNCKFSDLQGDAIEWNVAINDSDILISDHLIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVVLTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            1566..2561
                     /gene="gne"
     CDS             1566..2561
                     /gene="gnu"
                     /note="OgN16_1"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAV90522.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            2804..3697
                     /gene="galF"
     CDS             2804..3697
                     /gene="galF"
                     /note="OgN16_2"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAV90523.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRNGIEKLLSE"
     gene            4068..5153
                     /gene="rmlB"
     CDS             4068..5153
                     /gene="rmlB"
                     /note="OgN16_3"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAV90524.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKRFRFHHISTDEVYGDLPHPDEVNNNETLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KTLPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVENVKSGAYQSWIEQNYEGRK"
     gene            5153..6052
                     /gene="rmlD"
     CDS             5153..6052
                     /gene="rmlD"
                     /note="OgN16_4"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="BAV90525.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGSWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCVKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            6059..6988
                     /gene="rmlA"
     CDS             6059..6988
                     /gene="rmlA"
                     /note="OgN16_5"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAV90526.1"
                     /translation="MHLVRDGGARCIKRNDEMKTRKGIILAGGSGTRLYPVTMAVSKQ
                     LLPIYDKPMIYYPLSTLMLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPS
                     PDGLAQAFIIGEDFIGGDDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDP
                     ERYGVVEFDKNGTAISLEEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEIT
                     DINRIYMEQGRLSVAMMGRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCLEEIAY
                     RKGFIDAAQVKVLAEPLKKNAYGQYLLKMIKGY"
     gene            6993..7529
                     /gene="rmlC"
     CDS             6993..7529
                     /gene="rmlC"
                     /note="OgN16_6"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="BAV90527.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCAVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPESDRGIIWNDPDINIIWPNI
                     AEPLLSDKDKLAKNISEL"
     gene            7542..8780
                     /gene="wzx"
     CDS             7542..8780
                     /gene="wzx"
                     /note="OgN16_7"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAV90528.1"
                     /translation="MSLVRNTFWNLSGYIIPSIIAIPSLGFLARSLGPERFGLYTLAI
                     ALVGYASIFDAGLTRAVIREVSYFRNKDYEIRKIISNSTIILMFLGVIGGVFIYFGAN
                     HIVTFLRVEKLLENEAEVAVKLISLTLPLFLLNQVWLGILEGYEQFKKINIIKTFNSS
                     FIVGLPAVFVYCERSLSYAISGLLCARILSITITFFACKKYIITSGFLFDVRTAKRLL
                     NYGGWITVSNIISPLMAYLDRFFISNIMGASNVAFYTAPAEGVQRLSIFPGALSRAVF
                     PKLSRLASKQEKIKQKKVAYLLMTGAISPICIIAFFYADKIMTLWMGSDFSGLSASIL
                     QILLIGYFFNCIAQIPFASIQAAGKSKVTALLHLCEVLPYFAMLFSFIHLYGLIGAAI
                     AWSVRTTIDCFLLIWLNSKA"
     gene            8788..10008
                     /gene="wzy"
     CDS             8788..10008
                     /gene="wzy"
                     /note="OgN16_8"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAV90529.1"
                     /translation="MVALYIFTFFLATSVTYKFWQRLTYNSLVNIPLFIIIIVYQISG
                     ETFGFKELNENVYLYFSVFLILGSFIEICMVLLFRGLYNNQMPIKAAVNVPKTFCYLV
                     IFISFLVLFFASLFARQYGLVSEEFEGKMASGFVGHLLVFLMITPPFIYLAYANKKIT
                     KSLFFFCVSLVFICLFLKQVKSWIMIPIVYFFVMYLYFNKVNKKKITLYSLCAAITLF
                     MLFFLVYSLKAIIINPESNLLELFSQIYQHFLFYLFSGVGGFSEYLHANTNDLTDNWY
                     VLVLPVVNIINMITAGTMESAINSKIYIINYEIDRGSNVFTIYGTMWMYLKYYAFLFY
                     GFIVALQAFLYISRKNYVACSVFWMITSFGLFSWFDYYYFLLGAYEAPLYILVLAIIF
                     MGKKNEKRMLNSYS"
     CDS             9977..10999
                     /note="OgN16_9"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90530.1"
                     /translation="MKNECLTVILNWNGSEDTIACINSILLHPEIRTDIVIIDNDSCQ
                     VQYDKLHIELQVYECSDFIELSGFENGVEVVAVNSYIKNSSRIYLVKAKTNYGFSKAC
                     NAGAFFAAQLNYEYILFLNNDTEVEHNFLSLLIDDIKKENITAVIPQIRYFYDKNKIW
                     NCGGTISTYGSRNYYYSNQDYREINKFERYIPVSFATGCCILFRTREFLELGMFTEKF
                     FFGEEDIELSLRLLKMNKVILCDTESIIYHKVGASITGDKQKLLRKSYIHYLNRLVNM
                     KTYLGWKWFLWLVPSVTKIIVNLIKINKVNFCELCKFTYAIVIDAYKSKGVDKEKFER
                     TLKDGY"
     CDS             10989..12080
                     /note="OgN16_10"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90531.1"
                     /translation="MATKMKVLILSSACCIHTRKWCNGLAQKGLDVHLVSQQVPMEGY
                     SNDVTFHKLPYQGLKGYFLNGRALKKVIKKINPDVVNVHYASGYGTLAAIAGLKNFIM
                     SVWGSDVYDFPFTSPLHHKLVNYNLNRSSVICSTSEVMGEFVANNFSLKKNLSIQITP
                     FGVDVSKFKKKKLESNKTSFVIGTVKTLRPKYGIDNLIKAFAFLVNKGHNNISLRIAG
                     KGFMRAQLEELTRTLGVSDKVEFLGWVENDKVPSLLNTFDVYVAPSTLDSESFGVAIV
                     EASSCELPVIVTRVGGLPEVVIDNKTGIVVEPNDIELLSDAMEALINDQNLCSSLGET
                     GRKHVIEKYEWNYCVDKMISIYDKYRYKA"
     CDS             12081..13232
                     /note="OgN16_11"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf12"
                     /product="hypothetical protein"
                     /protein_id="BAV90532.1"
                     /translation="MFMMKNTCVVGSLSGIFTHSYIVGLINTGHKVCIINTSKRINYD
                     KYLDCSVLNLYPKKNEQINAKTKSNLKKNFLFCYFYELFLFFRSIFSHNADIDFFLKG
                     KDINTFVFFWGTSIRREFYCIKNIFKNIKDKPKFILDVATYPIRDYATENTPGSFLFF
                     DTKFFNEFDIVLSHSSYMDSFLQTKLSVDSYRINRFVCSFPNSAYFKGVIESSETTPK
                     KIIFLGTLDKSSSINDITNDIIKLADTGIDVYVQENKDYVNKNPRIKTFKPFSFEQII
                     NGEFSNFCSTFDGALVVYGPMSPLREKLTYPTRYALALLQGIPIFIPKKRFKSLEEIA
                     GGNKNNQIIYYSSIDEIATTILSFPSSLYRVDESMETKLNDLLVRKDQC"
     CDS             13510..14262
                     /note="OgN16_12"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf13"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90533.1"
                     /translation="MNIPAVSIIMPSFNSEKTIQAAIDSVRAQTYSSWELIISDDNSS
                     DSTVEIVEKNVLYEPRIILIQHSENRGAGFSRNDAISKARGKYIAFLDSDDIWHEKKL
                     EIQIRFMSERKLALSYTQYQKINMEGVKGKIIIPPDVTSYSELLKSNVIGCLTAVYDQ
                     EILGKMYMPLIRKRQDMALWLDILKKIEFAYCVPEVLAFYREGHNSLSSNKIKILSSQ
                     WDFYRGHLKFGIFRSAYYFSFYVFNALKKHKT"
     gene            14365..15771
                     /gene="gnd"
     CDS             14365..15771
                     /gene="gnd"
                     /note="OgN16_13"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAV90534.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLTGPKAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            15993..17186
                     /gene="ugd"
     CDS             15993..17186
                     /gene="ugd"
                     /note="OgN16_14"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAV90535.1"
                     /translation="MFNRKKSELMKITISGTGYVGLSNGILIAQNHEVVALDIVQAKV
                     DMLNKKQSPIVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFN
                     TSSVEAVIRDVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYD
                     NLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVA
                     YFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQS
                     VPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRI
                     KAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRD
                     LFGSD"
     gene            17276..18310
                     /gene="wzz"
     CDS             17276..18310
                     /gene="wzz"
                     /note="OgN16_15"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAV90536.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGS
                     AAPKVSEIQSMLIGRFSTTFSALAETLDNLEEPEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAQYIQQVDDQVNEELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIDNLDVDKLDIHTYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGSGIVLG
                     RNALRNYNAK"
     gene            complement(18481..19092)
                     /gene="hisI"
     CDS             complement(18481..19092)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAV90537.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLTTVIENLRKRHQ"
BASE COUNT     5652 a   3571 c   4206 g   5698 t
ORIGIN
        1 tagaggagaa cgcatgccat ttaaaaaact ctcccgacgc accttcctga cggcaagctc
       61 ggtgcttgcc ttcctccata cccctttcgc tcgcgcactt cccgcacgac aaagcgttaa
      121 tattaacgac tacaacccac acgactggat cgcctcattt aaacaagcct tcagcgaagt
      181 gcatacagtc gtcgtgcctg ccggattggt ttgtgacaat atcaacaccg gcatcttcat
      241 tcctcctggc aaaacgttac acatccttgg aagcctgcgc ggcaacggca gagggcgatt
      301 tgtcttacag gacggcagcc aggtgacagg ggagaagggc ggcagtatgc ataacatcac
      361 cctggatgtg cgcggttctg actgcaccat caaagggctg gcgatgagcg gctttggccc
      421 ggtaacgcag atttatatcg gcggcaaaaa caaacgcgtc atgcgtaacc tggccatcga
      481 taacctgacc gttagccacg ctaattacgc catcttacgc cagggatttc ataaccagat
      541 tatcggtgcc agcatcacca actgtaagtt cagcgactta caaggcgacg ccattgaatg
      601 gaacgtggcg attaacgaca gtgatattct catctctgac catctcatcg agcgcatcaa
      661 ctgtactaac ggcaaaatca actggggcat tggcataggc cttgcgggaa gcacttatga
      721 taataactac ccggaagacc aggcagtgaa aaactttgtc gtggccaata taacgggatc
      781 ggattgtcgg cagttgatac atgttgaaaa tggtaaacat tttgttattc gtaatatcaa
      841 agcccgcaat atcacgccgg atttcagtaa gaaagcgggt attgataacg cgacagtcgc
      901 tatttacggt tgtgacaatt tcgtgattga taatattgaa atgattaata gcgctgggat
      961 gttaatcggt tatggggtaa ttaaaggcaa atatctctcg ataccgcaaa atttccgagt
     1021 gaataatatt caactggata atactcatct tgcttataaa ttgcgcggca tccaaatctc
     1081 cgccggaaat gccgtttcct ttgtggtact gactaacatt gagatgaagc gtgcgtcatt
     1141 ggagctgcac aacaagccgc aacacctttt tatgcgcaat atcaaggtta tgcaggaatc
     1201 ctcagttgga cccgcattga gcatgaactt cgacatgcgc aaagacgttc gtggcgtgtt
     1261 tatggcgaaa aaagaaacac tgctgtctct tgcaaatgtt catgcggtga atgaaagagg
     1321 gcaaagctcc gtcgatatcg acaggattaa tcaccatatt gttaatgtgg aaaagattaa
     1381 ctttagattg ccggaacgga gggagtagat ttgcgaccat tcataaactt actggatata
     1441 atgttctaaa agattataat cctcacacta actgttaaag ctggggctgg gcagcattga
     1501 ctactcaggg aaggtactca tctttatctt gtgtttctta cgtaacaacc agactaaagt
     1561 gatttatgaa cgataacgtt ttgctcatag gagcttccgg attcgtagga acccgactac
     1621 ttgaaacggc aattgctgac tttaatatca agaacctgga caaacagcag agccactttt
     1681 atccagaaat cacacagatt ggcgatgttc gtgatcaaca ggcactcgac caggcgttag
     1741 ccggttttga cactgttgta ctactggcag cggaacaccg cgatgacgtc agccctactt
     1801 ctctctatta tgacgttaac gttcagggta cccgcaatgt gctggcggcc atggaaaaaa
     1861 atggcgttaa aaatatcatc tttaccagtt ccgttgctgt ttatggtttg aacaaacaca
     1921 accctgacga aaaccatcca cacgaccctt tcaaccacta cggcaaaagt aagtggcagg
     1981 cagaggaagt gctgcgtgaa tggtataaca aagcaccaac agaacgttca ttaaccatca
     2041 tccgtcctac cgttatcttc ggtgaacgca accgcggtaa cgtctataac ttgctgaaac
     2101 agatcgctgg cggcaagttt atgatggtgg gcgcagggac taactataag tccatggctt
     2161 atgttggaaa cattgttgag tttatcaagt acaaactgaa gaatgttgcc gcaggttacg
     2221 aggtttataa ctacgttgat aagccagacc tgaacatgaa ccagttggtt gctgaagttg
     2281 aacaaagcct gaacaaaaag atcccttcta tgcacttgcc ttacccacta ggaatgctgg
     2341 gtggatattg ctttgatatc ctgagcaaaa ttacgggcaa aaaatacgct gtcagctctg
     2401 tgcgcgtgaa aaaattctgt gcaacaacac agtttgacgc aacgaaagtg cattcttcag
     2461 gttttgtggc accgtatacg ctgtcgcaag gtctggatcg aactctgcag tatgaattcg
     2521 tccatgccaa aaaagacgac ataacgtttg tttctgagta atgcagacga gtcagagaaa
     2581 aaaggaccag gttggactac gattattcgg aaagaggtac tgattaaatt atcagtatgt
     2641 ggatttccga gtaatccgag taacccagat gctgatggtg tgtagtgtta tcgtaaggtt
     2701 taatcaaagt tatgcggaac aaagctttgg tatatagcca taacgtttgg ctaacctatg
     2761 gcaataattt acgcactatc agccacttag aggtaggaaa ataatgacga atttaaaagc
     2821 agttataccg gtagcgggtc ttgggatgca tatgttgcct gccactaagg cgatacccaa
     2881 agagatgcta ccgatcgtcg acaagccaat gattcagtac attgttgacg agattgtggc
     2941 tgcagggatc aaagaaatcc tcctggtaac tcacgcgtcc aagaacgcgg tcgaaaacca
     3001 cttcgacacc tcttatgaat tagaatctct tctcgaacaa cgtgtgaagc gtcaactgtt
     3061 ggcggaagta cagtccattt gcccgccggg cgtgaccatt atgaacgtgc gtcagggtga
     3121 acctttaggt ttaggccact ccattttatg tgcacgccct gccattggcg acaatccgtt
     3181 tgttgtggtg ttgccagacg tcgtgatcga cgacgccagc gccgacccgc tgcgctacaa
     3241 ccttgcagcc atgattgcac gtttcaacga aacgggccgc agccaggtgc tggcaaaacg
     3301 tatgccgggt gacctgtctg aatactccgt cattcagacc aaagaaccac tggatcgtga
     3361 aggtaaagtc agccgcattg ttgaattcat agaaaagccg gatcagccgc agacgctgga
     3421 ctcagacatc atggccgttg gtcgctatgt gctttctgcc gatatttggc cggaactgga
     3481 acgcacgcag cctggtgcat gggggcgtat tcagctgaca gatgccatcg ctgaactggc
     3541 gaaaaaacaa tctgttgatg caatgctgat gactggtgac agctacgact gcggtaaaaa
     3601 aatgggctat atgcaggcgt ttgtgaagta tggactacgc aatctgaaag aaggggcgaa
     3661 gttccgtaat gggattgaga agctgttaag cgaataatga aaatctgacc ggatgtaacg
     3721 gttgatgaga aaattataac ggtagtgaag attcgtagcg aaagtaattt gttgcgaata
     3781 ttcctgccgt tttttatata aacaatcaga ataacaacga gttagcaata ggattttagt
     3841 caaagttttc caggattttc cttgtttcca gagcggaatg gtaagacaat tagcgtttga
     3901 atttttcggg tttagcgcga gtgggtaacg ctcgtcacat cgtaggcatg catgcagtgc
     3961 tctggtagct gtaaagccag gggcggtagc gtgcattaat acctctatta atcaaactga
     4021 gagccgctta tttcacagca tgctctgaag taatatggaa taaaagagtg aagatacttg
     4081 ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata aataatacgc
     4141 aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa tcacttgcag
     4201 atgtttctga ttcagaacgc tatgttttcg aacatgcaga tatttgcgat gctgctgcaa
     4261 tggcacggat ttttgctcag catcagccgg atgcagtgat gcacctggct gctgaaagcc
     4321 atgttgaccg ttcaattaca ggtcctgcgg catttattga aaccaatatt gttggcactt
     4381 acgtcctttt agaagcagct cgtaattact ggtctgcgtt gaatgatgaa aagaaaaaaa
     4441 ggttccgctt ccatcatata tccactgacg aagtgtatgg cgacttaccc catccggatg
     4501 aagtaaataa caatgaaaca ttgccgttat ttacagaaac aacagcctat gcgccaagta
     4561 gtccgtactc tgcttcaaaa gcttccagtg atcatttggt acgcgcgtgg aaacggactt
     4621 atggtttacc gaccattgtg actaattgct ctaacaatta tggtccttat catttcccgg
     4681 aaaaattgat tccattggtt attctgaatg ctctggaagg taaaacatta cctatttatg
     4741 gtaaagggga tcaaattcgc gactggctgt atgttgaaga tcatgcgcgt gcgttatata
     4801 ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtgggcac aacgaaaaga
     4861 aaaacataga tgtagtgctc actatttgtg atttgctgga tgagattgta ccgaaagaga
     4921 aatcttatcg tgagcaaatc acttatgttg ccgatcgtcc gggacacgat cgacgttatg
     4981 cgattgatgc tgataagatt ggtcgcgaat tgggatggaa accacaggaa acgtttgaga
     5041 gcgggatacg taaaacggtg gaatggtacc tgtccaatac aaaatgggtc gaaaatgtga
     5101 aaagtggtgc ctatcaatcg tggattgaac agaactatga gggccgcaag taatgaatat
     5161 cctccttttc ggcaaaacag ggcaggtagg ttgggaacta cagcgtgccc tggctcctct
     5221 gggtaatttg attgctcttg atgttcactc cactgattat tgtggtgatt ttagtaatcc
     5281 tgaaggtgta gctgaaaccg taagaagcat tcggcctgat attattgtca acgcagccgc
     5341 tcacaccgca gttgacaaag cagaatcaga accggagttt gcacaattac ttaacgcgac
     5401 aagtgtcgaa gcgattgcga aagcagccaa tgaagtcggc tcctgggtta ttcactactc
     5461 tactgactac gtatttccgg gaaccggtga aataccatgg caggaggcgg atgcaaccgc
     5521 accgctaaat gtttacggtg aaaccaagtt agccggagaa aaagcattac aagagcattg
     5581 tgtgaagcac cttattttcc ggaccagctg ggtctatgca ggtaaaggaa ataacttcgc
     5641 caaaacgatg ttacgtctgg caaaagagcg tgaagaattg gccgttatta acgatcagtt
     5701 tggtgcgcca acaggtgcag agctgctggc tgattgtacg gcacatgcca ttcgtgtcgc
     5761 actgaataaa ccggaagtcg cgggcttgta ccatctggta gctagtggta ccacaacgtg
     5821 gcacgattat gctgcgctgg tttttgaaga ggcgcgcaaa gcaggcattc cccttgcact
     5881 caacaagctc aacgcagtac caacaacagc ctatcctaca ccagctcgtc gtccacataa
     5941 ctctcgcctt aatacagaaa aatttcagca gaactttgcg cttgtcttgc ctgactggca
     6001 ggttggcgtg aaacgaatgc ttaacgaatt atttacgact acagcaattt aatagttttt
     6061 gcatcttgtt cgtgatggtg gagcaagatg tattaaaagg aatgatgaaa tgaaaacgcg
     6121 taaaggtatt attttggcgg gtggttctgg tactcgtctt tatcctgtga cgatggccgt
     6181 cagtaaacag ctattaccga tttatgataa accgatgatc tattacccgc tttctacact
     6241 gatgttagcg ggtattcgcg atattctgat tatcagtacg ccacaggata ctcctcgttt
     6301 tcaacaacta ctgggtgacg gtagccagtg ggggctaaat cttcagtaca aagtgcaacc
     6361 gagtccggat ggtcttgcgc aggcgtttat tatcggtgaa gactttattg gtggtgatga
     6421 ttgtgctttg gttcttggtg acaatatctt ctacggtcac gatctgccga agttaatgga
     6481 cgtagctgtt aacaaagaaa gtggtgcaac agtatttgcc tatcacgtta atgatcctga
     6541 acgctatggc gttgttgagt ttgataaaaa cggtacggca attagcctcg aagaaaaacc
     6601 gctacaacca aaaagtaatt atgcggtaac cgggctttat ttctatgata acgacgttgt
     6661 ggaaatggcg aaaaatctta agccttctgc ccgcggtgaa ctagaaatta ccgatattaa
     6721 ccgtatttat atggaacagg ggcgtttatc tgttgccatg atgggccgtg gttatgcatg
     6781 gctggacacg gggacacatc aaagcctgat tgaggcaagc aacttcattg ctacaattga
     6841 agaacgtcag gggctgaaag tttcctgtct agaagaaatt gcttaccgta aagggtttat
     6901 tgatgctgcg caggtgaaag tattagctga accgttgaag aaaaatgctt atggtcagta
     6961 cctgctaaaa atgattaaag gttattaata aaatgaacgt aattaaaaca gagattccag
     7021 acgtattaat tttcgaaccg aaagtttttg gtgatgagcg tggtttcttt atggaaagct
     7081 ttaatcagaa agttttcgaa gaagctgtag gacgtaaggt tgaatttgtt caggataacc
     7141 attcgaagtc tagtaaaggt gttttacgcg ggctacatta tcagttagaa ccgtatgcgc
     7201 aagggaaatt ggtacgttgc gctgttggtg aggtttttga tgtagctgtt gatattcgta
     7261 aatcgtcgcc tacctttggt aaatgggttg gggtgaattt atctgctgag aataagcggc
     7321 aattgtggat ccctgaggga tttgcacatg gttttttagt gctgagtgag actgcggaat
     7381 ttttatataa aacgacgaat tattatcatc ctgagagtga tagagggatt atttggaatg
     7441 atccagatat aaatattatt tggccaaata ttgcagagcc tctcttatct gacaaagata
     7501 aactagctaa aaatatcagc gaactttaag cgagagaatg agtgagtcta gttcgaaata
     7561 cattctggaa tttaagtggt tatataatcc cctcgattat cgccattcct tctcttggtt
     7621 ttttagccag aagtcttggg ccagagagat ttggtctata cactctggcg attgcactcg
     7681 taggttatgc aagcatattt gatgccggac taacaagagc agtaatcaga gaagtttctt
     7741 attttaggaa taaagattac gaaataagaa agataatatc taattccaca ataatattga
     7801 tgtttttagg tgttattggc ggagtcttta tatattttgg tgcaaaccat attgtcactt
     7861 tcttaagagt tgagaaatta ctggaaaatg aagctgaggt ggcagtaaaa ctaattagtt
     7921 taactttacc attgttttta ttaaatcagg tatggttggg cattttggag gggtatgagc
     7981 aatttaaaaa aataaatatt attaaaacat ttaatagctc ctttattgtt gggcttcccg
     8041 cagtgttcgt ttattgtgaa aggagtttat cttatgccat ttcaggtctg ctttgtgcaa
     8101 gaatattatc tataacaata actttttttg catgtaaaaa gtatattatc acatcaggat
     8161 ttttatttga tgttagaact gctaaaaggt tattaaatta tggggggtgg attacagtta
     8221 gcaatattat tagtccatta atggcttatc ttgataggtt ttttatatca aatataatgg
     8281 gagcttcgaa tgtggcattt tatactgctc ctgcagaggg agtacagcgg ttgtcaatat
     8341 ttcccggagc tctatcaaga gctgtttttc ctaagttgag tcgattagcc agtaaacaag
     8401 aaaaaataaa acaaaaaaaa gttgcttatt tattaatgac aggggctatt tccccaatat
     8461 gcataattgc tttcttttat gctgataaaa taatgactct ttggatgggc agtgacttct
     8521 ctggattgtc ggcttctatc ttacagatat tgttaattgg atattttttt aactgtattg
     8581 ctcaaattcc atttgcttcc atacaggcgg caggaaaatc aaaggttaca gcactgttgc
     8641 acttatgtga ggttttgccg tattttgcaa tgttgttttc tttcatacat ttatacgggt
     8701 taataggtgc tgctattgca tggtccgtaa gaacaacaat agattgtttt ttgttaatat
     8761 ggctaaatag caaggcttga ggtaatcatg gttgcgttat atatatttac tttctttttg
     8821 gctacatcgg ttacttataa gttttggcaa cgcttaacgt ataactcact tgttaacata
     8881 ccgcttttta tcataataat tgtttatcaa atttctggtg agacatttgg ctttaaagaa
     8941 ttaaatgaaa atgtatattt atatttttct gtatttttaa tattgggctc ttttattgaa
     9001 atttgcatgg tgctgctttt tcgtgggcta tataacaatc agatgccaat taaggcagcc
     9061 gttaatgtgc caaaaacatt ttgttatttg gttattttta tttctttttt ggttttattt
     9121 tttgcttcgt tatttgcacg gcaatatggt ttggtaagtg aagaatttga agggaaaatg
     9181 gcatcaggat ttgtcggtca tttgttggtt ttcctgatga taactccccc ttttatttat
     9241 ttagcatatg caaataaaaa aataacaaaa agtttatttt tcttttgtgt ctccttggtt
     9301 tttatatgtc ttttcctaaa acaagtaaaa tcatggatta tgattcccat tgtttatttt
     9361 tttgtcatgt acctttattt taataaagtt aataaaaaga aaattacact atactcttta
     9421 tgtgcagcaa tcaccctttt tatgttattt tttttagttt actcgctcaa agcaatcatc
     9481 ataaaccccg aaagtaatct gttagagtta tttagtcaaa tataccagca ttttcttttt
     9541 tatcttttta gtggcgtggg aggtttttct gagtatttac acgcgaatac taatgacctc
     9601 actgacaact ggtatgtttt agtattgcca gtagttaata ttattaatat gattactgca
     9661 ggtaccatgg agtcagcaat taactctaaa atctatatta ttaactatga aatagataga
     9721 gggtctaatg tatttacaat atatggtact atgtggatgt accttaaata ttatgcattt
     9781 ctattttatg gatttatagt cgcattacaa gcgtttctat acatatcacg aaaaaattat
     9841 gtggcctgta gtgttttttg gatgatcacc agttttggat tattttcttg gtttgattat
     9901 tattattttt tattaggagc ttatgaggca cctctgtata ttttagtgct tgctattatt
     9961 tttatgggta aaaaaaatga aaaacgaatg cttaacagtt attcttaatt ggaatggttc
    10021 tgaagataca attgcctgca taaatagtat attattacat ccggaaattc gtactgatat
    10081 agtcataatt gataatgatt catgtcaagt gcagtatgat aaattacata ttgagttgca
    10141 ggtatatgag tgttctgatt ttattgaatt atccgggttt gaaaatggtg ttgaagtcgt
    10201 agcagttaat tcttatatta aaaattcatc tcggatttat ttagttaaag caaaaactaa
    10261 ttatggattt tcaaaggcat gtaatgctgg tgcatttttt gctgcgcaat taaattatga
    10321 atatattctt tttcttaaca atgatacgga agttgagcat aactttctct cattactaat
    10381 tgatgatatt aaaaaagaaa atataacggc tgtaattcct caaataagat atttttacga
    10441 taagaacaaa atatggaatt gcggaggaac aatttctact tatggaagtc ggaattatta
    10501 ttattcaaat caagattatc gagaaattaa taaatttgaa cgatacatac cagtcagttt
    10561 tgctacagga tgttgtattt tatttagaac cagagagttt cttgaattag gaatgtttac
    10621 ggagaaattt ttctttggcg aagaagacat agaattatca ttacgtttac tgaaaatgaa
    10681 caaggttata ttgtgtgaca cagagtcaat tatttatcac aaagttggcg caagcattac
    10741 gggtgataaa caaaaattat tgagaaaatc ttatattcat tatttaaata gactagtaaa
    10801 tatgaaaaca tatttgggat ggaaatggtt tctttggttg gtcccatcgg tgactaaaat
    10861 tattgttaat cttattaaaa tcaataaggt taatttttgt gagctctgta aatttactta
    10921 tgcaattgta attgatgcct ataaaagcaa aggtgtagat aaagagaaat ttgaacgtac
    10981 tttgaaagat ggctactaaa atgaaagtat tgatattgtc ttctgcatgt tgtattcata
    11041 caagaaaatg gtgtaatgga ttagctcaga aaggacttga tgtgcattta gtatcacagc
    11101 aagttccaat ggagggatac agtaatgatg tgacatttca caaattgcca tatcaaggtc
    11161 ttaaaggata tttcctgaat ggaagggctt taaagaaagt aattaaaaaa attaatcctg
    11221 atgtcgtaaa tgttcactac gcctctggct atggaacttt agctgcaatt gcggggttaa
    11281 agaattttat tatgtctgtt tggggctctg atgtttacga ttttccattc acatctccat
    11341 tgcatcataa attagttaac tataacttaa ataggtcgtc agttatttgt tcaacaagtg
    11401 aggttatggg agagtttgtc gcgaataatt ttagcctcaa aaaaaatctt agtattcaaa
    11461 tcacaccttt tggtgttgat gtcagtaaat ttaagaaaaa aaaattagaa tctaataaaa
    11521 catcattcgt tattggcaca gtcaaaacat tgaggcctaa atatggtatt gataatttaa
    11581 tcaaggcatt tgcgtttttg gtaaataagg gacataacaa tatctccctg agaatcgctg
    11641 ggaaaggatt catgagggcg cagttagaag agttaactcg aacattgggt gtttctgaca
    11701 aagttgagtt tttagggtgg gtggaaaatg ataaagtccc atctttatta aatacatttg
    11761 atgtttatgt tgcgccaagt acattagata gtgaaagttt tggagtagca attgtcgaag
    11821 cttcatcatg cgaactacca gttattgtaa cgcgagtagg aggattacct gaagtcgtaa
    11881 tcgataataa aacaggtatt gttgtcgaac caaatgacat tgaattatta agtgatgcaa
    11941 tggaagcatt aattaatgat cagaatctat gctcatcatt aggggaaaca ggtagaaaac
    12001 atgtcataga aaaatatgag tggaactatt gcgttgacaa aatgattagt atttatgata
    12061 agtatcgtta taaagcttag gtgttcatga tgaaaaatac atgtgttgtt ggctccttaa
    12121 gtggtatttt tacgcatagt tatatcgttg gattgatcaa taccggtcat aaagtatgca
    12181 ttattaatac ctcaaaaaga ataaattacg ataaatatct tgattgcagt gttcttaatc
    12241 tttatccaaa aaaaaatgaa caaattaatg ctaaaacaaa atcaaactta aagaaaaatt
    12301 tcctattttg ttatttttat gaactttttt tgttttttcg ctctattttt tctcataatg
    12361 ctgatattga tttttttttg aaagggaagg atattaatac ttttgtgttt ttttggggga
    12421 catcaataag aagagaattt tattgtatta aaaatatctt taaaaatatc aaagataaac
    12481 ctaaattcat attagatgtg gcaacgtatc ctattcggga ttatgcaaca gaaaatacac
    12541 ctggttcttt tctatttttt gataccaaat ttttcaatga gttcgatatt gttttatcac
    12601 attcatcata tatggactcg tttctacaaa ctaaacttag tgtagatagc tatcgtatca
    12661 atcgttttgt ttgttcattc cctaacagcg cctattttaa aggcgtaatt gagagttcag
    12721 aaacaacacc aaaaaaaatc atctttcttg gaactcttga taagtcatcg tctattaatg
    12781 acattacaaa tgatatcatt aaattagctg atactggaat tgatgtttat gttcaagaaa
    12841 ataaagatta tgttaataaa aatccaagaa taaaaacctt caaacccttt tcatttgaac
    12901 aaattataaa tggtgaattt tctaattttt gttcaacatt tgatggtgca ttagtggttt
    12961 atggaccaat gtctccattg cgagaaaaat taacgtatcc tacacgatat gcgttagcct
    13021 tattacaagg cattccaatt tttataccta aaaaaagatt taaatcatta gaagaaattg
    13081 caggtggaaa caagaataat caaataattt attattcatc cattgatgaa atagcaacaa
    13141 caatattatc cttcccaagt tctttataca gagtagatga aagcatggaa acaaaattaa
    13201 atgatctttt ggtaagaaaa gatcaatgtt agatagtttg catacataaa tattagccag
    13261 ttatttatgg tttcaatcct taattgaata tcctaaatat catcagctag tgtgtttaaa
    13321 gcagtgtttt ttatcaataa taacaagaca tttttattat aagttttaaa tgataacgtt
    13381 aaaataacat gtttataatg gttttaatct tttagagata ttttggttgg aattaattat
    13441 aataatgtaa ccagtaatgg catacgcgat ttatatcttg agggttatta ttaataaaat
    13501 aggtgttata tgaatattcc tgctgtatca ataattatgc catcatttaa tagtgagaaa
    13561 actattcaag ctgcaatcga ctcggttaga gcgcaaacat attctagttg ggagttgatt
    13621 atatctgatg ataactccag tgattccacc gtggaaattg ttgaaaaaaa cgtgttgtat
    13681 gagccccgca ttattctgat acaacattct gaaaatagag gtgcaggttt ttcgagaaat
    13741 gatgcaatct ctaaagctcg tggtaaatat attgcatttc ttgatagtga tgatatatgg
    13801 catgaaaaga aattagaaat acaaattcgc tttatgtcag agcgaaagct tgcgttatcc
    13861 tatacacagt atcaaaaaat taatatggag ggggttaaag gtaaaataat tattcctcca
    13921 gacgttactt catattctga gttattaaaa tccaatgtca ttggttgttt aacagcggta
    13981 tatgaccaag aaattttggg aaaaatgtat atgcccctaa ttcgaaaacg tcaagacatg
    14041 gctctatggt tagatatcct aaagaagatt gagtttgcat actgtgtccc tgaagttttg
    14101 gcattttatc gggaagggca taacagttta tcttcaaata aaataaaaat attatcatcc
    14161 caatgggact tttaccgagg gcatttaaag tttggtatat tcagaagtgc atattacttt
    14221 tcgttttatg tttttaatgc attgaaaaag cataaaacat agaaagaatt catgctattc
    14281 aaataaacat aaattgagtt aatatttaaa ggcactatag tcgcgcacaa cctcgcgctg
    14341 aacaccccct gacaggagta aacaatgtca aagcaacaga tcggcgtcgt cggtatggca
    14401 gtgatggggc gcaaccttgc gctcaacatc gaaagccgtg gttataccgt ctctattttc
    14461 aaccgttccc gtgagaaaac ggaagaagtg attgccgaaa atccaggcaa gaaactggtt
    14521 ccttactata cggtgaaaga gtttgttgaa tctctggaaa cgcctcgtcg catcctgtta
    14581 atggtgaaag caggtgcagg cacggatgct gctattgatt cccttaagcc gtacctcgat
    14641 aaaggtgaca tcatcattga tggtggtaac accttcttcc aggacactat tcgtcgtaat
    14701 cgtgagcttt ctgccgaagg ctttaacttc atcggtactg gtgtttccgg cggtgaagaa
    14761 ggcgcgctga aaggaccttc cattatgcct ggtggccaga aagaagccta tgaactggtt
    14821 gcgccgatcc tgaccaaaat cgctgccgta gctgaagatg gcgaaccgtg cgttacctat
    14881 attggcgcag acggcgcagg tcactatgtg aagatggttc acaacggtat tgaatacggc
    14941 gatatgcagc tgattgctga agcctattct ctgcttaaag gtggcctgaa cctttccaac
    15001 gaagaactgg cgcagacctt taccgagtgg aataacggtg aactgagcag ctacctgatc
    15061 gacatcacca aagatatctt caccaaaaaa gatgaagacg gtaactacct ggttgatgtg
    15121 atcctggatg aagcggctaa caaaggtacc ggtaaatgga ccagccagag cgcactggat
    15181 cttggcgaac cgctgtcgct gattaccgag tctgtgtttg cacgttatat ctcttctctg
    15241 aaagatcagc gtgttgccgc atctaaagtt ctcactggcc cgaaagcgca gccagcaggc
    15301 gataaggctg agtttatcga gaaagttcgt cgtgcgctgt atctgggcaa aatcgtttct
    15361 tatgctcagg gcttctctca gttgcgcgcc gcgtctgaag agtacaactg ggatctgaac
    15421 tacggcgaaa tcgcgaagat tttccgtgct ggttgcatca tccgtgcgca gttcctgcag
    15481 aaaatcaccg atgcatatgc cgaaaatccg cagatcgcta acctgctgct ggctccgtac
    15541 ttcaagcaaa tcgccgatga ctaccagcag gcgctgcgtg atgtcgtcgc ttacgcggta
    15601 cagaacggta tcccggttcc gaccttcgcc gctgcggttg cctattatga cagctaccgt
    15661 tccgctgttc tgcctgcgaa cctgatccag gcacagcgtg actatttcgg tgcgcatact
    15721 tataagcgca ttgataaaga aggtgtgttc cataccgaat ggctggatta atctgattta
    15781 aatcaattaa tcaatgcaag gcccggagaa aacctccggg cttttttatt atacaaagcg
    15841 gcaggttatg gccttttttt ataatttata ggtaaaaacg cgatataata cagcgccgca
    15901 cagcagaatc gctgccttga cagttcatct gcatcagcgt taaaaattcc gcagtagatg
    15961 aagttgtggt ggtggattaa tgaccacttt aaatgtttaa ccggaagaag tcagagctaa
    16021 tgaaaataac aatttcagga acaggttatg ttggtctttc aaatggtatt ctgattgcgc
    16081 aaaaccacga agtggttgca ctggatatcg ttcaggccaa agtggacatg cttaacaaga
    16141 agcagtcacc gattgttgat aaggagattg aagagtatct ggcgactaaa gatctcaatt
    16201 tccgcgctac gacagataag tatgacgcgt ataaaaatgc cgattacgtt attattgcca
    16261 cgcctaccga ttatgacccg aaaacaaatt attttaatac ctcaagcgtg gaagcggtca
    16321 tccgtgatgt gacagaaatt aatcccaacg cggtaatggt gattaaatct acaattccgg
    16381 taggtttcac caaatcaatt aaagaacgtc tgggtatcga taaccttatt ttctcccccg
    16441 aattcctccg cgaaggtaaa gccctttacg ataatctcca tccgtcacgt attgtcatcg
    16501 gtgagcgttc ggaacgcgca gaacgttttg ctgcgttgtt acaggaagga gcgattaagc
    16561 aaaatatccc gaccctgttc accgattcaa ccgaagcaga agcgattaaa ctgtttgcta
    16621 atacctatct ggcgatgcgc gttgcatact ttaacgagct ggatagctat gcagaaagtt
    16681 taggtctgaa tactcgtcag attatcgaag gtgtttgtct cgatccgcgt attggcaatc
    16741 attacaacaa tccgtcgttt ggctatggtg gttattgtct gccaaaagat acgaaacagt
    16801 tactggcgaa ctaccagtct gtgccgaata acctgatctc agcaattgtc gatgctaacc
    16861 gcacgcgtaa agattttatt gccgatgcca ttttgtcacg caaaccgcaa gtggtgggta
    16921 tttatcgcct gattatgaag agcggttcag ataacttccg tgcgtcttcc attcagggga
    16981 ttatgaagcg tatcaaagcg aaaggcgttg aagtgatcat ttacgagccg gtgatgaaag
    17041 aagactcatt cttcaactct cgcctggaac gtgatctcgc caccttcaaa caacaagccg
    17101 acgtcattat ctctaaccgt atggcagaag agcttaagga tgtggcagac aaagtctaca
    17161 cccgcgatct ctttggcagt gactaacatc ctgttatcag ggcgattttc gccctgattc
    17221 tctcatgttc cctttgtaat aattcattat ttttatcatt tatcctatag cattcatggc
    17281 gattatcgct aaactatggc gacttaaaat tatttcgtcc gttagggtaa tgatgagagt
    17341 agaaaataat aatgtttctg ggcaaaacca tgacccggaa cagattgatt tgattgattt
    17401 actggtccaa ctatggcgtg gcaagatgac tatcatcatt tctgtcattg ttgctattgc
    17461 cctggcgata gggtatctgg ccgttgccaa agaaaagtgg acttccacag ctatcgttac
    17521 tcaacctgat gtgggacaaa tcgctggtta taacaatgcc ataaatgtga tttatggttc
    17581 ggcagcgccg aaagtgtctg agatccagtc gatgttaatt ggtcgtttca gcacgacatt
    17641 ttctgcatta gcagaaacgc tggataatct cgaagaacct gaaaaactga ccattgagcc
    17701 taccgttaaa aatcagtcat tacctttagc ggtttcttat gtgggtgaaa cacctgaagg
    17761 tgcgcagaaa cagctggcgc agtatatcca gcaagttgac gaccaggtaa atgaagaact
    17821 ggaacaagac ctgaaagaca acatcgcgct gcaaatgaaa aacttgcagg attcgctgaa
    17881 gacgcaggaa gtagtcgctc aagaacagaa agatctgcgt atccgtcaga ttcaggaagc
    17941 gttgcagtat gcgaatcagg cgcaggtgac aaagccgcag attcagcaga cacaggacgt
    18001 aacccaagat actatgttcc ttttggggag cgaagcgctg gagtcgatga ttaagcacga
    18061 ggcgactcgt ccgttggtgt tctcgccaaa ctactatcag actcgtcaaa acctgctgga
    18121 tatcgacaat cttgacgttg ataaacttga tattcatact taccgctatg taatgaaacc
    18181 gacgttacct attcgtcgcg atagcccgaa aaaggcaatt accttgattc tggcggtgct
    18241 gctgggcggc atggttggct caggtattgt gctggggcgt aacgctctac gcaattacaa
    18301 cgcgaaataa tccttgaagc tttaaagaaa acgggcaggg tggtgacacc ttgcccgttt
    18361 ttttgccgga tgcgatgtat ccagcttacg tgtgcagaga gaatagtagg cccgataagc
    18421 ttgcgcatcg ggcaatggct ccggatgcga caacaacatc gcatccgctt accccgcaac
    18481 tcactgatgc cgtttacgca ggttctcaat caccgtcgtt aaatccagcc cctgatcctg
    18541 caacagcacc agcaggtgat acatcaagtc agatgcctcg ttggtcagct caaagcggtc
    18601 atgtaccgtt gctgccagtg cggtttccac gccttcttcg cccactttct gcgcaatgcg
    18661 cttggtgccg ctggcataca gtttggcggt ataggaggtt tccgggtcgg cagatttgcg
    18721 ttcggcgagc agttgttcca gttgatacag gaacagccac tggtgagcgg tgtcgccgaa
    18781 gcagctgctg gtacctttgt ggcaggttgg cccgattgga tttgccagca ccagtaacgt
    18841 gtcgttgtcg cagtccggag caatactcac cacattgaga aaattgcccg acgtttcgcc
    18901 tttggtccac agtcgctgtt tagtgcgcga gaagaaggtg actttgccgc tttcgatggt
    18961 tttatctaag gcttccgggt tcatatagcc cagcattaac acttcaccgg ataccgcgtg
    19021 ttgcacaatc accggcatca gtccgtcggt tttttcccag tccagttcgc gacgttgttg
    19081 ttctgttaac atatcctgat ctccacgccc tgtgttgcca ggtacgc
//
