LOCUS       LC177546               19591 bp    DNA     linear   BCT 15-DEC-2018
DEFINITION  Escherichia coli DNA, O-antigen biosynthesis gene cluster OgN2,
            strain: E1657.
ACCESSION   LC177546
VERSION     LC177546.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., von Mentzer,A., Kikuchi,T. and Thomson,N.R.
  TITLE     An untypeable enterotoxigenic Escherichia coli represents one of
            the dominant types causing human disease
  JOURNAL   Microb Genom 3 (2017) In press
  REMARK    DOI:10.1099/mgen.0.000121
            Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19591)
  AUTHORS   Iguchi,A., von Mentzer,A. and Thomson,N.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2016) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuenkibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..19591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E1657"
                     /db_xref="taxon:562"
                     /note="Escherichia coli O-antigen biosynthesis gene
                     cluster OgN2"
     gene            14..1408
                     /gene="wcaM"
     CDS             14..1408
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAV90409.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGKEGGGMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMTNSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            1571..2476
                     /gene="galF"
     CDS             1571..2476
                     /gene="galF"
                     /note="OgN2_1"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAV90410.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            2848..3933
                     /gene="rmlB"
     CDS             2848..3933
                     /gene="rmlB"
                     /note="OgN2_2"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAV90411.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            3933..4832
                     /gene="rmlD"
     CDS             3933..4832
                     /gene="rmlD"
                     /note="OgN2_3"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="BAV90412.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVAS
                     GTTTWYDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            4890..5768
                     /gene="rmlA"
     CDS             4890..5768
                     /gene="rmlA"
                     /note="OgN2_4"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAV90413.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKRFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            5773..6321
                     /gene="rmlC"
     CDS             5773..6321
                     /gene="rmlC"
                     /note="OgN2_5"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="BAV90414.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYHPESDRGIRWDDEELSITWPRG
                     NELNLSNKDQQHPSFQNCQCFE"
     CDS             6377..7357
                     /note="OgN2_6"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90415.1"
                     /translation="MINTMSRKNNSVDIVLATYNGEKYISEQITSILNMSGFNTYVNS
                     IIICDDNSTDRTLEIIKAIVPSEKLCIIKNTTDRNLGPVSNFERGLLVSNSEYVMLSD
                     QDDVWNNQKLLACMDAIKNCNEERPFLIFSDLEVVDRDLNLIAESFQEYQSIPYDWYK
                     NINNLLIQNVAPGCTMFFNKKLISMALPLPDNCIMHDWWLILVATLHGEVYFLKDKLI
                     KYRQHGNNQVGAKKIGFIETAKDFYKLKQKAEKNLKETILQMNTLSEMFEENLSQNTK
                     DKIKAWNKCFFEYNNFFVIFSLMHKYKLRKSTLIKTLGVYYLMLKHQGAK"
     CDS             7354..8097
                     /note="OgN2_7"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90416.1"
                     /translation="MIYISIVSHNHGNVILQLGCVKKLSKKYNVIITDNTGEVYLEKF
                     CKSNGVWYIKNAERKGFGENNNQNFQFARNKLSMRDDDYFLVLNPDVYIEDKELEQAL
                     SLIIKEKIKASTINLMKEKNTYDNNIRCYPGFFDFIESYLFGKNRTIINKSNINDNQY
                     VDWASGSFLIILVEVYERVGGFDEHYFMYCEDLDLCRRIHSLTGEKIFYINEVKALHF
                     AAHNNRRLFSKHFMWHLQSIIRYCLISKY"
     gene            8113..9126
                     /gene="wzy"
     CDS             8113..9126
                     /gene="wzy"
                     /note="OgN2_8"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAV90417.1"
                     /translation="MFYWLMLFFVSTTAFVAKGKIGNSITVLAAIITAGFIAPGVSQD
                     YYNYLNGYYLTTPSLYTEPLSKIIFRLSFQLNQPITVSFLIFAIISLLIKYNALIKLN
                     IPISVFFLVYCSKLYLLLDLTQVRAGVAVAICLIALFHRVNGRNKLSLLYIIIGFLFH
                     YSAIMFLIIFIFSKRHANNAMWLCGVVLSILFSALDIKGILTSLFITVHVPTNYLAYL
                     SIESDFKVNPFSILSIVNLMIFVIFSFYKGLNNDVLFNLCYKLYGISIISFYLFLHFP
                     VLSFRISEFFLIYQILLIGLAYNKTIVNQRWVFLVLLTFYSALQLYMTYNVAQVIMPY
                     KFV"
     CDS             9233..10519
                     /note="OgN2_9"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="putative dehydrogenase"
                     /protein_id="BAV90418.1"
                     /translation="MRVSMNIDNIKIGIIGLGYVGLPLAVEFGKKYNTVGFDIKQNRI
                     DELKKQMDSTLECTADELASAKFLHYSCEIEALKACNIFIVTVPTPIDKYKRPDLSPL
                     VSASKMLSKVIKNGDIIIFESTVYPGATEEICVPQIEAGSGLVFNKDFFIGYSPERIN
                     PGDKNHRVTNIMKVTSGSTPEAATFVDKIYSSIITAGTYKASSIKVAEAAKVIENTQR
                     DLNIALINELAIIFNKLGIDTEDVLKAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKA
                     QEIGYHPEVILAGRRINDDMARYVASQLIKEMIKAGILIDNANILIMGLSFKENCPDI
                     RNTKIIDIISELKDYNLNVDVYDPWVSEDEALQEYGMEIHSTKPSKKYEGIIFAVAHE
                     QFKNMQSIEIKELQSTKCVTYDLKYILNKSDATIRL"
     CDS             10534..11565
                     /note="OgN2_10"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /product="putative epimerase"
                     /protein_id="BAV90419.1"
                     /translation="MTRYEKTIEQLKVKPKTWLITGVAGFIGSNLLETLLRLNQTVVG
                     LDNFATGHQRNLDEVQQNVTPEAWQKFTMIEGDIRDYETCMKAVDNVNYVLHQAALGS
                     VPRSINDPITTNEVNVSGFLNMLQAAKCRDVESFVFAASSSTYGDHTALPKIEHTIGK
                     PLSPYAITKYVNELYAEVFAKMYGFKSIGLRYFNVFGKRQDPNGAYAAVIPKWISAML
                     MDKDIYINGDGETSRDFSFIENTVQMNILAATADDSAKGEVYNVAYGERTSLNELVEF
                     IKNDLGSNGIKYAGNIIYRDFRKGDVRHSLADISKAREMLGYQPQFDIKTGLRQAMPW
                     YINLFKKAE"
     CDS             11662..12414
                     /note="OgN2_11"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf12"
                     /product="putative glycosyltranslocase"
                     /protein_id="BAV90420.1"
                     /translation="MKKIAIYKVVIGAYDSITLDSLKTAESTSSLCFEHFLISDQYIE
                     VPETWTLIQISRKFVSPAVENRYYKMGVPSIFDDYDYSIYLDGNIVINDDLTNLIKKI
                     IIDDHYIYAYPHFKNSTIKEEIENCFVFSRISWYDMLKIKRKLKYNLNEKVGFECGVL
                     IRKKRNKELDDLFKTWFELYFNNIRRDQFYFSIALKKHGLICREIGVNDIRTGKGFFS
                     LHPHKNKISIMNKIYIALKMRIYSKLHNMKGI"
     gene            12414..13655
                     /gene="wzx"
     CDS             12414..13655
                     /gene="wzx"
                     /note="OgN2_12"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAV90421.1"
                     /translation="MRSLYLFISSVSNLLFIVLIQLITLIKIGPSSDTDALFAGMTIP
                     SVILSVLVSSLTNVLVPLFVSSKSVLKDLWAQIYLFSIGSAFIVIPLISFSPLWLGCV
                     FSGFDVKTLSLTQHIITVQFIVIPFSIASAIFTAFFNANSKFISAEAIPAICSLIIFP
                     FIFPAITSYGVLAVTYIYAIKILMTFVMQIMKIGGPIKLSFNDIDIKGAFSRIKYLIL
                     GSVYYKSGPVIDRHILSYSVAGSLSLFVLVQQLLSMANLIFTKTIIIPDITMMNKVAV
                     QDNKIFKHWLMTRVFILFAFGLVLYFFAFILGEQIINLFLKIFNFQKFDVHIIWMLTM
                     ALFGGFIGDSIATLVSSSFYSKGDTKTPSFLSIVTFTIFIPLKFISYHYAGVYGLAFA
                     SSIYSLINMSVMFLIIMLRLK"
     CDS             13652..14539
                     /note="OgN2_13"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf14"
                     /product="hypothetical protein"
                     /protein_id="BAV90422.1"
                     /translation="MIVFIGWTADYDRIMIQQLREHFEIKNIAYPEGRLFFRLNKITK
                     FNLVRLFLKLHSLAFPPDAILIFKDSDGYGYHRHLDLFKQKKILIVRNIITSELALFF
                     KDSFDEVHSFDKAQCDKYDFKYMRQIFPLSNVYKNGSLANDSCYFVGLDKNRLSIIDS
                     IGQALSYHGIKPSFFVIKDKTSYKHSGFYSNCAISYKENINKVQENKYILEVNTVGQV
                     GLTLRSLESIFYSRKLISNNIDLMNCDFYDKSRIFIFNNPNDLFSSDFDEFIRLPYKP
                     VENVILEKYKASNIFKKLT"
     gene            14907..16313
                     /gene="gnd"
     CDS             14907..16313
                     /gene="gnd"
                     /note="OgN2_14"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAV90423.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPDKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            16558..17724
                     /gene="ugd"
     CDS             16558..17724
                     /gene="ugd"
                     /note="OgN2_15"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAV90424.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQADKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVVKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            17814..18848
                     /gene="wzz"
     CDS             17814..18848
                     /gene="wzz"
                     /note="OgN2_16"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAV90425.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGP
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQPLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIENLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(18945..19556)
                     /gene="hisI"
     CDS             complement(18945..19556)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAV90426.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     5931 a   3522 c   4114 g   6024 t
ORIGIN
        1 tagaggagaa cgtatgccat ttaaaaaact ctcccgacgc accttcctga cggcaagctc
       61 ggcgcttgcc ttcctccata cccctttcgc tcgcgcactt cccgcccgac aaagcgttaa
      121 cattaacgac tacaacccac acgactggat cgcctcattt aaacaagcct tcagcgaagg
      181 gcaaacagtc gtcgtgcctg ccggattggt ttgtgacaat atcaacaccg gcatcttcat
      241 tcctccaggc aaaacgttac acatcctggg aagcctgcgc ggtaacggca gagggcgttt
      301 tgtcttacag gacggcagcc aggtgacagg gaaggagggc ggcggtatgc ataacatcac
      361 cctggatgtg cgtggctctg actgcaccat caaagggctg acgatgagcg gctttggtcc
      421 ggtaacgcag atttatatcg gcggcaaaaa caaacgggtc atgcgcaacc tgaccatcga
      481 taacctcact gtcagccacg ctaattacgc catcttacgc cagggatttc ataaccagat
      541 aatcggtgcc aacatcacca actgtaaatt tagtgattta cagggcgacg ccattgaatg
      601 gaacgtggcg attaacgacc gtgatatctt gatctccgac catgtcatcg agcgcatcaa
      661 ctgtactaat ggcaaaatca actggggcat cggcataggt cttgcgggaa gtacctacga
      721 taacaactac ccggaagacc aggcagtgaa aaactttgtc gtggcgaata tcacgggatc
      781 ggattgtcgg cagttgatcc atgttgaaaa tggtaaacat tttgttattc gtaatatcaa
      841 agcccgcaat atcacgccgg atttcagtaa gaaagcaggt attgataacg ccacagtcgc
      901 tatttacggt tgtgacaatt tcgtgattga taatattgaa atgactaata gcgccgggat
      961 gttaatcggt tatggggtaa ttaaaggcaa atatctctcg ataccgcaaa atttccgagt
     1021 gaataatatt caactggata acacccatct tgcttataaa ttgcgcggca tccaaatctc
     1081 cgccgggaat gctgtctcct ttgtggcact gactaacatt gagatgaagc gtgcatcgct
     1141 ggagttacac aacaaaccgc aacatctttt tatgcgtaat atcaaggtga tgcaggaatc
     1201 ctcagttgga ccagcattga gcatgaactt cgacatgcgc aaagacgttc gcggcgtctt
     1261 tatggcgaaa aaagaaacac tgctgtctct tgcaaatgtt catgcggtga atgaaaaagg
     1321 gcaaagctcc gtcgatatcg acagagttaa tcaccatatt gttaatgtgg aaaagattaa
     1381 ctttagattg ccggaacgga gagagtagat ttgcgaccat tcctggaaaa atggagccat
     1441 acttaggaac aatgctactg caatccacaa cgaagcggcg taacatcaca agtaattcag
     1501 taatcaattc agggtaattg atgctggcga aaaaaatcga acaagctata attcagcaac
     1561 cattttacag gtggatgaaa taatgacgaa tttaaaagca gttattcctg tagcgggtct
     1621 tgggatgcat atgttgcctg ccactaaggc gattcccaaa gagatgctac cgatcgtcga
     1681 caagccaatg attcagtata ttgttgacga gattgtggct gcagggatca aagaaatcct
     1741 cctggtaact catgcgtcca agaacgcggt cgaaaaccac ttcgacacct cttatgaatt
     1801 agaatctctt cttgaactgc gcgtgaagcg tcaactactg gcggaagttc agtccatctg
     1861 cccgccgggc gtgaccatta tgaacgtccg tcagggcgaa cctttaggtt taggtcactc
     1921 cattttgtgt gcacgacccg ccattggtga caacccattt gtcgtggtgc tgccagacgt
     1981 tgtgatcgac gacgccagtg ccgatccgct gcgttacaac ctcgctgcca tgattgcgcg
     2041 tttcaatgaa acgggccgta gccaggtgct ggcaaaacgt atgccgggtg acctctctga
     2101 atactccgtt attcagacca aagaaccact ggatcgtgaa ggtaaagtca gccgcattgt
     2161 tgaatttatc gaaaaaccgg atcagccgca gacgctggac tcagacatca tggccgtggg
     2221 ccgttatgtg ctttctgctg atatttggcc ggaactagaa cgcactcagc ctggtgcatg
     2281 ggggcgtatt cagctgactg atgccatcgc tgaactggcg aaaaaacagt ccgttgatgc
     2341 catgctgatg actggagaca gctacgactg cggtaaaaaa atgggttata tgcaggcatt
     2401 tgtgaagtat ggactacgca acctgaaaga aggggcgaag ttccgtaaag ggattgagaa
     2461 gctgttaagc gaataatgaa aatctgaccg gatgtaacgg ttgataagaa aattataacg
     2521 gcagtgaaga ttcgtggcga aagtaatttg ttgcgaatat tcctgccgtt gttttatata
     2581 aacaatcaga ataacaacga gttagcaata ggattttagt caaagttttc caggattttc
     2641 cttgtttcca gagcggattg gtaagacaat tagcgtttga atttttcggg tttagcgcga
     2701 gtgggtaacg ctcgtcacat cgtaggcatg catgcagtgc tctggtagct gtaaagccag
     2761 gggcggtagc gtgcattaat acctctatta atcaaacaaa gagccgctta tttcacagca
     2821 tgctctgaag taatatggaa taataaagtg aagatacttg ttactggtgg cgcaggattt
     2881 attggttctg ctgtagttcg tcacattata aataatacgc aggatagtgt tgttaatgtc
     2941 gataaattaa cgtacgccgg aaacctggaa tcacttgctg atgtttctga ttccgaacgc
     3001 tatgtttttg aacatgcgga tatttgcgat gcagctgtaa tggcacggat ttttgctcag
     3061 catcagccgg atgcagtgat gcacctggca gctgaaagcc atgttgaccg ttcaattaca
     3121 ggccctgcgg catttattga aaccaatatt gttggtactt atgtcctttt agaggccgct
     3181 cggaattact ggtctgctct tgatggcgac aagaaaaata gtttccgttt tcatcatatt
     3241 tctactgacg aagtctatgg tgatttgcct catccagatg aagtaaataa tacagaagaa
     3301 ttacccttat ttactgagac gacagcttac gcgccaagca gcccttattc cgcatccaaa
     3361 gcatccagcg atcatttagt ccgtgcgtgg aaacgtacct atggtttacc gaccattgtg
     3421 actaattgtt cgaataacta cggtccttat cactttccgg aaaaattgat tccactagta
     3481 attcttaatg ctctggaagg taaggcatta cctatttatg gcaaagggga tcaaattcgc
     3541 gactggctgt atgttgaaga tcatgcacgt gcgttatata ccgtcgtaac cgaaggtaaa
     3601 gcgggtgaaa cttataacat tggtggacac aacgaaaaga aaaacatcga tgtagtgctc
     3661 actatttgtg atttgttgga tgagattgta ccgaaagaga aatcttaccg cgagcaaatt
     3721 acttatgttg ccgatcgtcc gggacacgat cgccgttatg ccattgatgc tgagaagatt
     3781 ggtcgcgaat tgggatggaa accacaggaa acgtttgaga gcgggattcg gaagacagtg
     3841 gaatggtacc tgtccaatac aaaatgggtt gataatgtga aaagtggtgc ctatcaatcg
     3901 tggattgaac agaactatga gggccgccag taatgaatat tctccttttc ggcaaaacag
     3961 ggcaggtagg ttgggaacta cagcgtgctc tggcaccttt gggtaatttg attgctcttg
     4021 atgttcactc cactgattat tgtggtgatt ttagtaatcc tgaaggtgtg gctgaaaccg
     4081 tcaaaaaaat tcgccctgat gttattgtta atgctgcggc tcataccgca gtagataagg
     4141 ctgagtcaga acccgaattt gcacaattac tcaatgcgac cagcgttgaa tcaattgcaa
     4201 aagcggctaa tgaagttggg gcctgggtaa ttcattactc aactgactac gtatttccgg
     4261 gaaccggtga aataccatgg ctggagacgg atgcaaccgc accgctaaat gtttacggtg
     4321 aaaccaagtt agccggagaa aaagcgttac aggaacattg cgcgaagcat cttattttcc
     4381 gtaccagctg ggtatacgca ggtaaaggaa ataacttcgc caaaacgatg ttgcgtctgg
     4441 caaaagagcg tgaagaatta gcggttatta acgatcagtt tggtgcacca acgggtgctg
     4501 agctgctggc tgattgtacg gcacatgcta ttcgtgttgc actgaaaaaa ccagaagtcg
     4561 caggcttgta ccatttggta gccagtggta ccacaacctg gtacgattat gctgcgctgg
     4621 tttttgaaga ggcgcgcaaa gcaggcattc cccttgcact caacaagctc aacgcagtac
     4681 caacaactgc ctatcctaca ccagctcgtc gtccacataa ctctcgcctt aatacagaaa
     4741 aatttcagca gaactttgcg cttgtcttgc ctgactggca ggttggcgtg aaacgaatgc
     4801 tcaacgaatt atttacgact acagcaattt aatagttttt gcatcttgtt cgtgatggtg
     4861 gagcaagatg aattaaaagg aatgatgaaa tgaaaacgcg taaaggtatt attttagcgg
     4921 gtggttctgg tacacgtctt tatcctgtga ctatggctgt cagtaaacag ctgttaccga
     4981 tttatgataa accgatgatc tattacccgc tctctacact gatgttggcg ggtattcgcg
     5041 atattctgat tatcagtacg ccacaggata ctcctcgttt tcaacaactg ctgggtgacg
     5101 ggagccagtg ggggctaaat cttcagtaca aagtgcaacc gagtccagat ggtcttgcgc
     5161 aggcatttat catcggtgaa gagtttatcg gtggtgatga ttgtgctttg gttctaggtg
     5221 ataatatctt ttacggtcac gatctgccga agttaatgga tgtcgctgtt aacaaagaaa
     5281 gtggtgcaac ggtatttgcc tatcacgtta atgatcctga acgctacggt gtcgttgagt
     5341 ttgataaaaa cggtacggca ataagcctgg aagaaaaacc gttacaacca aaaagtaatt
     5401 atgcggtaac cgggctttat ttctatgata acgacgttgt cgaaatggcg aaaaatctta
     5461 agccttctgc ccgcggtgaa ctggaaatta ccgatattaa ccgtatttat atggaacagg
     5521 ggcgtttatc cgttgccatg atggggcgtg gatatgcatg gctggacacg ggaacacatc
     5581 aaagtcttat tgaagcaagc aactttattg caacaattga agaacgtcag ggactgaaag
     5641 tttcctgtcc ggaagaaatt gcttaccgta aacggtttat tgatgctgag caggtgaaag
     5701 tattagccga accgctgaag aaaaatgctt atggtcagta tctgctcaaa atgattaaag
     5761 gttattaata aaatgaacgt aattaaaact gaaattccag acgtactgat ttttgaaccg
     5821 aaagtttttg gtgatgagcg tggtttcttt tttgagagct ttaaccagaa ggtttttgag
     5881 gaagctgtag gccgcaaagt tgaatttgtc caggataacc attcgaagtc ttgtaaaggt
     5941 gtcttacgcg gattgcatta tcagttagaa ccttatgcac aagggaaatt ggtgcgctgc
     6001 gttgttggtg aagtttttga tgttgcggtt gatattcgta aatcgtcacc aacttttggc
     6061 aaatgggttg gggtgaattt atctgctgag aacaagcgcc agttgtggat acctgaagga
     6121 tttgcacatg gttttttggt gctgagtgag actgcggagt ttgtatataa aactacaaat
     6181 tattatcatc cggaaagtga tagagggatc cgatgggatg atgaggaatt atctattacg
     6241 tggcctagag gaaatgaact aaacttatca aataaagatc agcaacatcc gagctttcaa
     6301 aactgtcagt gttttgaata actgtttcga ggagatattt tatgacgtaa aatatctcct
     6361 aataattggt taggtattga taaatacaat gtctcgcaaa aataattctg ttgacatagt
     6421 gcttgcaact tataacggtg aaaaatacat ttctgaacaa ataacatcaa tactcaacat
     6481 gtcaggtttc aatacatatg ttaatagtat tattatttgt gatgataatt caactgatcg
     6541 cacattagaa ataataaaag ccattgttcc atccgaaaaa ctatgtatta ttaaaaatac
     6601 aacagatagg aatttggggc cagtcagtaa tttcgaacgg ggtttattgg tatccaattc
     6661 tgaatatgtg atgctttcag atcaagatga tgtatggaat aatcaaaaat tattagcttg
     6721 catggatgca ataaaaaact gcaatgaaga aagacctttt ctgatattta gtgatcttga
     6781 ggttgtagat agggatttaa atcttattgc agaatcattt caagagtacc aatcaatacc
     6841 ctatgattgg tataaaaata ttaataatct tttgattcaa aatgtggcac ctggttgtac
     6901 tatgtttttt aataaaaaat taatttcaat ggcattacca ctccctgata attgcattat
     6961 gcatgattgg tggcttatat tggtagcaac attgcatggg gaagtttatt ttttgaagga
     7021 taaattaata aagtataggc aacatgggaa taaccaagta ggtgcaaaaa aaattgggtt
     7081 cattgaaact gcaaaggatt tttataagct aaaacaaaaa gcggaaaaaa atctaaaaga
     7141 gacaatattg caaatgaata cactctctga aatgtttgag gagaatctat cacaaaatac
     7201 aaaagacaaa ataaaagcgt ggaataaatg tttttttgaa tataataatt tttttgttat
     7261 attttctttg atgcacaagt acaagttaag aaaatcgaca ttaattaaaa cgttgggtgt
     7321 ttattatctt atgttaaagc atcaaggggc aaaatgattt atatatcaat tgtatcacat
     7381 aatcatggca atgttatttt gcaactaggc tgtgtgaaaa agttaagtaa aaaatacaat
     7441 gttatcatta cagataatac tggtgaggtt tatcttgaga aattctgcaa gagtaatggt
     7501 gtttggtata taaaaaatgc tgaaagaaaa gggtttgggg aaaataacaa tcaaaacttt
     7561 caatttgcaa gaaataaact ttcaatgagg gatgatgatt attttttagt tttaaatcct
     7621 gatgtctata ttgaagataa agaattagaa caagctttat cattaataat aaaagaaaaa
     7681 ataaaggcat caacaataaa cttaatgaaa gagaaaaaca catatgacaa caatatccgc
     7741 tgttatcctg gctttttcga ttttattgaa tcgtacttgt tcggtaaaaa tcgcactatc
     7801 attaacaagt cgaatataaa tgataatcaa tatgttgatt gggcttcagg ttcattctta
     7861 atcattctcg ttgaagtcta tgaaagagta ggtggatttg atgagcatta ttttatgtat
     7921 tgtgaagact tggatttatg ccgccgtatt cattctctga ccggtgagaa aattttttat
     7981 attaatgagg tgaaagcact acattttgct gcgcataata atcggcgttt gttttcaaaa
     8041 catttcatgt ggcaccttca gagcattata cgatattgtc taatttcaaa atattaaaca
     8101 agtgaataat atatgtttta ttggttgatg cttttttttg tatctacaac tgcttttgtt
     8161 gcaaaaggaa aaattggtaa tagtattaca gtattggctg ctattattac tgctggattt
     8221 atagctccgg gtgtaagtca agactattat aattatttaa atgggtatta tctcacaaca
     8281 ccttctttat atactgagcc gttatctaaa ataatattca gattgtcttt ccaattaaat
     8341 cagccaataa cagtaagttt tttgattttt gcaataatct ctctactgat taaatacaat
     8401 gcgttaatta aactaaatat cccgatttct gtattttttc ttgtctattg ttctaaactt
     8461 tatttattat tagatttaac tcaagttaga gctggcgttg cagtcgcaat ttgcctaata
     8521 gcattgtttc atcgagttaa tgggagaaat aaattaagcc tactttacat tattataggt
     8581 tttttgtttc actatagcgc aattatgttt ttaattattt ttatcttcag taaaagacat
     8641 gcaaataatg ctatgtggct ctgcggtgtg gttttatcaa ttctattttc agcactagat
     8701 attaaaggta tccttacatc attatttatt acagtgcatg tgccaactaa ctatttagct
     8761 tatttgagta tagaatcgga ttttaaagta aacccattta gtattctttc tattgtgaat
     8821 ttaatgatat ttgttatttt tagtttctat aaggggctta ataatgatgt gttatttaat
     8881 ctttgctata aactttatgg tatatcaata atatcgttct atctatttct tcatttccct
     8941 gttctatcgt ttagaattag tgagtttttt ttaatatacc agattttact gattggactg
     9001 gcatataata aaactatagt aaatcagaga tgggtttttc ttgttttatt gacattttat
     9061 tctgcattac aattatatat gacatataat gtcgctcaag taattatgcc ctataaattt
     9121 gtttaatcta gtgagcgatt tgaattatta tagtatttga tataatatca ccactattgt
     9181 tttatgtgtt ttaacaattg gttgtttgtc tcttataaaa tatgagtaaa tagtgagagt
     9241 ttctatgaat attgataata taaaaatagg catcattggt cttggttatg ttggcctgcc
     9301 tttggcggtg gaattcggga aaaaatataa tactgttggt tttgatatta aacaaaacag
     9361 aattgacgaa ttaaaaaaac aaatggatag tacactggag tgtactgcgg atgagcttgc
     9421 tagtgcgaaa tttcttcact attcatgtga aatcgaagca ctcaaagcgt gtaatatatt
     9481 tattgttact gtacctacgc caattgataa atataaacga ccggatttaa gtcccttggt
     9541 tagtgcttca aaaatgttaa gcaaggttat taaaaatggc gatatcatta tatttgaatc
     9601 gacggtctac ccaggcgcaa cagaagaaat atgtgttcct cagattgaag ctggttccgg
     9661 attagttttc aacaaagatt tttttatagg ctatagccca gagagaatca atccaggaga
     9721 taaaaatcat agagtaacaa atattatgaa agtaacttct ggttcaacac cagaagcagc
     9781 aacgtttgtg gataaaattt actcctcaat tataacagcc gggacataca aggcatcatc
     9841 tattaaggtg gctgaggccg ctaaggtaat tgaaaatacg caacgagatc ttaatattgc
     9901 actaataaac gaattggcta tcatcttcaa taaattaggt attgatactg aggatgtttt
     9961 aaaggcagcg ggtactaaat ggaattttct cccttttcgt cccggcctgg ttggtggtca
    10021 ttgtatcggg gttgacccat attatttaac gcataaagct caagaaattg gttatcatcc
    10081 agaggttatt ttggctggta gacgtattaa tgatgatatg gcacgttatg ttgcatctca
    10141 acttataaag gaaatgatta aggcaggtat tctaatagat aatgctaata ttttaattat
    10201 ggggttatct tttaaagaaa attgccctga tattcgcaat acgaaaatta ttgatataat
    10261 atcagaatta aaagattata atttgaatgt cgatgtttat gacccttggg taagtgaaga
    10321 tgaggcattg caggaatatg gcatggaaat tcattctact aaaccttcta agaaatatga
    10381 aggcataatt tttgctgtag cccatgagca attcaaaaat atgcaatcca ttgaaatcaa
    10441 ggagctgcag tccacaaaat gtgtgacata tgatttgaaa tatattctta ataaatcaga
    10501 tgcaacaatt agactttaac taggtgaata tctatgacta gatatgaaaa aactattgag
    10561 caattaaaag ttaaacctaa gacttggttg ataacaggag ttgctggctt cattggctca
    10621 aatttacttg aaacactttt aagattaaat cagacagtag ttggtttgga taattttgct
    10681 acaggacatc agcgaaatct tgatgaagtc cagcaaaacg ttacacctga agcatggcaa
    10741 aaatttacaa tgatcgaagg tgatatacgt gattatgaga catgtatgaa ggccgttgat
    10801 aatgttaact acgtattgca ccaggctgca cttggttcag tgcctcgttc aattaatgat
    10861 cctataacaa ctaatgaagt taatgtctct ggcttcttga atatgttaca agctgccaag
    10921 tgtagagatg tagagagttt tgtttttgcc gcttcaagtt caacatatgg tgatcatacc
    10981 gctctgccca aaatagaaca tacaattggt aaaccattat ctccctatgc tatcacaaaa
    11041 tatgtaaatg aattatatgc ggaagttttc gcaaaaatgt atggttttaa aagcattggt
    11101 ttgagatatt tcaatgtctt tggtaaaagg caagatccaa atggtgcata tgcagcagta
    11161 atacccaaat ggatatcggc aatgctcatg gataaagata tttatatcaa tggtgatggg
    11221 gaaactagta gagatttttc atttattgaa aatactgttc aaatgaatat tcttgctgct
    11281 acagcagatg atagtgcaaa aggagaagtt tacaacgttg cttatggtga gagaacatca
    11341 ttaaacgaat tagttgaatt tataaaaaat gaccttggca gtaatggaat aaaatatgca
    11401 gggaatatta tatatcggga tttcagaaaa ggtgatgtga gacattctct agctgatatt
    11461 agtaaagcta gagaaatgct cggctatcaa cctcagtttg atattaaaac tggtttaaga
    11521 caggcaatgc cctggtatat aaacttattt aaaaaagcag aatgacatac gtttctaact
    11581 agttatttta tattttggca gataatctgc caaaatataa tgcatttatt atctattacc
    11641 atcattatgt tacgtatata aatgaaaaaa atagcaatct ataaagttgt aattggagct
    11701 tatgattcaa taacattgga tagccttaag acagctgaaa gtacaagttc gttatgtttt
    11761 gagcattttc tcatttccga ccagtatatt gaagtgccag aaacatggac attgatacaa
    11821 atttcaagaa aatttgtttc tccagctgtg gaaaataggt attataaaat gggagtacca
    11881 tcaatatttg atgattatga ctatagtata tatcttgatg gtaatattgt aatcaatgat
    11941 gatttaacta acttaataaa aaaaatcatt attgatgatc attacatata tgcttatcct
    12001 cattttaaaa attcaactat caaagaagaa atagaaaatt gctttgtttt ttctcgtata
    12061 agttggtatg acatgcttaa aataaagcgt aaattaaaat ataatctaaa tgaaaaagta
    12121 ggctttgaat gtggtgtcct aataagaaaa aaaaggaata aagaattaga tgatttattt
    12181 aagacttggt ttgaactgta ttttaacaat ataaggcgag atcaatttta tttttcaatt
    12241 gcccttaaaa aacatggatt aatttgcagg gaaattggtg ttaacgatat tagaacggga
    12301 aaaggttttt tttcccttca tccacacaaa aataaaatat caattatgaa taagatttat
    12361 attgctctta aaatgagaat atattcgaaa ttgcataaca tgaaagggat ataatgcgtt
    12421 ctttatattt atttatttct tctgtttcta acttgttatt tattgttctc attcagttaa
    12481 taacactaat taaaatagga ccttctagcg atacagatgc tttatttgcc ggtatgacta
    12541 taccttctgt tatcctgtct gtattagtta gttcattaac taatgttctg gttccattat
    12601 ttgtatcttc aaagagtgtt ttaaaagatc tttgggcgca gatatactta ttttcaatcg
    12661 gctcagcatt tatagttata ccattaatat cctttagtcc attatggttg ggatgtgtat
    12721 tttctggttt tgatgtaaaa acattatctt taacgcaaca tattattacg gtccaattca
    12781 tcgtaattcc tttttctatc gcatcagcaa tattcactgc cttttttaat gcaaactcaa
    12841 aatttatttc tgcagaagcg attccagcaa tttgttcttt aattatattt ccatttattt
    12901 tcccagctat aacttcttat ggtgtgttgg ctgttacgta tatttatgca ataaaaatac
    12961 ttatgacatt cgttatgcaa ataatgaaaa taggaggccc aattaaatta tcctttaatg
    13021 atatagacat caagggagcc ttttcccgca ttaaatattt gattttaggt tctgtctatt
    13081 ataaatcagg tcccgttatt gacagacata ttctttctta ttcagttgct ggctctctct
    13141 ctctttttgt tttagtccag caattgcttt caatggcaaa cttaattttc actaaaacaa
    13201 taattattcc tgatataaca atgatgaata aggtggctgt acaggataat aaaatattta
    13261 aacattggtt aatgacgagg gtttttattt tatttgcctt tggtttggtt ctatatttct
    13321 ttgcctttat attaggggaa cagataataa atttattttt aaaaatattt aactttcaga
    13381 aatttgatgt ccatattatc tggatgttaa ctatggcgtt atttggtggg tttatagggg
    13441 attctattgc aacacttgtc tcttcaagtt tttattctaa aggtgatacc aaaacaccgt
    13501 cttttttatc aattgtaaca tttactatat ttattccttt aaaatttata tcttatcatt
    13561 atgctggggt ttatggatta gcattcgcta gtagtattta ttctttgata aatatgtcgg
    13621 ttatgttttt aattatcatg ttgaggctta aatgatagtt tttattggct ggacggcaga
    13681 ttatgaccgt ataatgatac agcaattaag agaacatttc gaaataaaaa acattgccta
    13741 tcctgaaggg cgattgtttt ttagattaaa caaaatcaca aaatttaatc ttgttcgatt
    13801 atttttaaag ttacatagtt tggcgtttcc acctgatgca attctgattt ttaaagattc
    13861 agatggatac ggctatcata gacatttaga tttatttaag caaaaaaaga tattgatcgt
    13921 aaggaatata atcacatcag aattagcctt gttttttaag gatagttttg atgaagtcca
    13981 ttcctttgat aaagcacaat gtgataaata tgactttaag tatatgcggc aaatatttcc
    14041 tttgtctaat gtttataaga atggttcact tgcaaatgat agttgttatt ttgtcgggct
    14101 tgataaaaat aggttgtcaa taatcgattc aattggtcaa gctctcagtt atcatggcat
    14161 taaaccatct ttctttgtta ttaaggataa aacctcttac aagcattcag gtttctatag
    14221 taattgtgct ataagttata aggagaatat aaataaagta caagaaaata aatatattct
    14281 tgaggtaaac acagttgggc aagttggatt aacattgcgt tctttggaat ctatttttta
    14341 ttcacgaaaa ttaatatcga acaatattga cttgatgaat tgtgattttt atgataaatc
    14401 ccggatattc atttttaata atccaaacga tctgttctct tcagattttg atgagttcat
    14461 ccgtttacca tataaacctg tagaaaatgt tattttagaa aaatacaaag catctaatat
    14521 ctttaaaaag ctaacttagt agaaattata taaaattaac ttgaactatt atgcatgcaa
    14581 tctttgataa tataaagttg tcgcaattaa tattattaca ttcttgtctg ttaaatctaa
    14641 attgtttgta tttaaaacta taaactaccg ttacttacaa actttaattt attgttgatt
    14701 gtcaattctt aaggtagtta ttatgtgagt cacagtataa gtgaattggt ctatctgtat
    14761 aacaattatg atttaaatga gatatctaaa ttaatatcaa atatctcatt taaattatga
    14821 cttttattca cgaaatctac gttctaatac atagaactat ctgaactatt atttatgata
    14881 gtttctcacc ctgacaggag taaacaatgt caaagcaaca gattggcgta gtcggtatgg
    14941 cagtgatggg gcgcaacctt gcgctcaaca ttgaaagccg tggttatacc gtctctattt
    15001 tcaaccgttc ccgtgagaaa acggaagaag tgattgccga aaatccagac aaaaaactgg
    15061 ttccttacta tacggtgaaa gagttcgttg aatctctgga aacgcctcgt cgcatcctgt
    15121 taatggtgaa agcaggtgca ggcacggatg ctgctattga ttccctcaag ccatacctcg
    15181 ataaaggtga catcatcatt gatggaggta acaccttctt ccaggacacc attcgtcgta
    15241 accgtgagct ttctgccgaa ggctttaact tcattggtac cggtgtctcc ggtggtgaag
    15301 aaggcgcgct gaaaggtcct tccattatgc ctggtggaca gaaagaagcc tatgaactgg
    15361 ttgcgccgat cctgaccaaa atcgccgcag tggctgaaga cggtgagcca tgcgttacct
    15421 atattggtgc cgatggtgca ggtcactatg tgaagatggt tcacaacggt attgaatacg
    15481 gcgatatgca gctgattgct gaagcctatt ctctgcttaa aggtggcctg aatctcacca
    15541 acgaagaact ggcgcagacc tttaccgagt ggaataacgg tgaactgagc agctacctta
    15601 tcgacatcac caaagatatc ttcaccaaaa aagatgaaga cggtaactac ctggttgatg
    15661 tgatcctgga tgaagcggct aacaaaggta ccggtaaatg gaccagccag agcgcactgg
    15721 atctcggcga accgctgtcg ctgattaccg agtctgtgtt tgcacgttat atctcttctc
    15781 tgaaagatca gcgtgttgcc gcgtctaaag ttctctctgg cccgcaagcg cagccagctg
    15841 gcgacaaggc tgagttcatc gaaaaagttc gtcgtgctct gtatctgggc aaaatcgttt
    15901 cttacgccca aggcttctct cagctgcgtg ctgcgtctga agagtacaac tgggatctga
    15961 actacggcga aatcgcgaag attttccgtg ctggctgcat catccgtgcg cagttcctgc
    16021 agaaaatcac tgatgcatat gccgaaaatc cgcagatcgc taacctgctg ctggctccgt
    16081 actttaaaca aattgccgat gactatcagc aggcgctgcg tgatgttgtt gcttatgcag
    16141 tacagaacgg tatcccggtt cccaccttcg ccgctgcggt tgcctattat gacagctacc
    16201 gtgccgctgt tctgcctgcg aacctgatcc aggcacagcg tgactatttc ggtgcacata
    16261 cttataagcg cattgataaa gaaggtgtgt tccatactga atggctggat taatctgact
    16321 taaccaacaa taaattgagg cccggcatat attgctgggc tttttttgcc aataatctta
    16381 tagactaaat ttcactgctt aatattaact taataaatat cagctattct tataaagaaa
    16441 atctgaattg tttttcgttg cgttgaccat ctgataacgt agcgttaaaa cttttagctc
    16501 ttatcaggat gctaaaaaca tcatgattca tagttaagtt aattctgaga gcatgaaatg
    16561 aaaatcacca tttccggtac tggctatgta ggcttgtcaa acgggcttct aatcgcacaa
    16621 aatcatgagg ttgtggcatt agatatttta ccgtcacgcg ttgctatgct gaatgatcgg
    16681 atatctccta ttgttgataa ggaaattcag cagtttttgc aagcagataa aatacacttt
    16741 aatgccacat tagataaaaa tgaagcctac cgggatgctg attatgtcat catcgccact
    16801 ccaaccgact atgatcctaa aactaattat ttcaatacat ccagtgtaga atcagtaatt
    16861 aaagacgtag ttgagataaa tccttatgcg gttatggtcg tcaaatcaac ggttcccgtt
    16921 ggttttaccg cagcgatgca taagaaatat cgtactgaaa atattatttt ctctccggaa
    16981 tttctccgtg aaggtaaagc cctttacgat aatctccatc cgtcacgtat tgtcattggt
    17041 gagcgttcag aacgcgcaga acgttttgct gcgttgttac aggaaggcgc gattaagcaa
    17101 aatatcccga ccctgtttac cgattcaacc gaagcagaag cgatcaaact gtttgctaat
    17161 acctatctgg cgatgcgcgt agcatacttt aatgaactgg atagctatgc agaaagttta
    17221 gggctgaata ctcgccagat tatcgaaggc gtttgtctcg atccgcgtat tggcaaccat
    17281 tacaacaatc cgtcgtttgg ttatggtggt tattgtctgc cgaaagatac caagcagtta
    17341 ctggcgaact accagtctgt gccgaataac ctgatttcgg caattgtcga tgcaaaccgt
    17401 acacgtaaag attttattgc cgatgccatt ttgtcacgca agccgcaagt agtgggtatt
    17461 tatcgtctga ttatgaagag cggatcagat aacttccgtg cgtcttccat tcaggggatt
    17521 atgaaacgta tcaaggcgaa aggcgttgaa gtgatcatct acgaaccggt gatgaaagaa
    17581 gattcattct tcaactctcg gctggaacgt gatctcgcca ctttcaaaca acaagccgac
    17641 gtcattattt ccaaccgtat ggcagaagag cttaaggatg tggcagacaa agtctacacc
    17701 cgcgatcttt ttggcagcga ctaacatcat gttatcaggg cgattttcgc cctgattctc
    17761 ttatgttccc tttgtaataa ttcattattt ttatcattta tcctatagca ttcatggcga
    17821 ttatcgctaa actatggcga cttaaaatta tttcgtccgt tagggtaatg atgagagtag
    17881 aaaataataa tgtttctggg caaaaccatg acccggaaca gattgatttg attgatttac
    17941 tggtgcagtt gtggcgtggc aagatgacaa ttatcatctc tgtcattgtg gctattgccc
    18001 tggctatagg gtatctggcc gttgccaaag aaaagtggac ttccacagct atcgttactc
    18061 aacctgatgt ggggcaaatc gctggttata ccaacgcgat gaatgtgatt tacggtccgg
    18121 ctgtaccgaa agtctccgac attcaggctt cacttatcgg acgttacagc actgcattct
    18181 cagcattagc ggaaacgctg gataaccagg aagaagcaga gaagctgacc attgagccta
    18241 ccgttaaaaa ccagccgtta ccactggcgg tgtcttatgt tggtgaaaca cctgaaggtg
    18301 cgcagaagca gctggcgaag tatatccagc aagttgacga ccaggtaaac gaagaactgg
    18361 aaaaagacct gaaagacaac atcgcgctgc gtatgaaaaa cttgcaggat tcgctgaaga
    18421 cccaggaagt ggttgcgcag gagcagaaag atctgcgtat ccgtcagatt caggaagcgt
    18481 tgcagtatgc gaatcaggcg caggtgacaa agccacaggt tcagcagact gaagatgtga
    18541 cgcaagatac gttgttcctt ttggggagcg aagctctgga gtcgatgatt aagcatgagg
    18601 cgactcgtcc gttagtgttc tcaccaaact actatcagac acgtcaaaac ctgctggata
    18661 tcgaaaactt aaaggttgat gatcttgata ttcatgctta ccgttatgtg atgaaaccga
    18721 cgttacctat tcgtcgcgat agcccgaaaa aggcaattac cttgattctg gcggtgctgc
    18781 tgggcggcat ggttggcgcg gggattgtct tggggcgtaa cgctctgcgt aattacaacg
    18841 cgaagtaatc ttttcggttt taaagaaaaa gggcagggtg gtgacacctt gcccgttttt
    18901 ttttccggat gctacaacaa tatcgcatcc gcttaccccg caactcactg atgccgttta
    18961 cgcaggttct caatcaccgt cgttaagtcc agatcctgat cctgcaacag caccagcagg
    19021 tgatacatca aatcagacgc ctcgttggtg agctcaaagc ggtcatgtac tgtcgctgcc
    19081 agtgcggttt ccacgccttc ttcgcccact ttctgcgcaa tgcgtttggt gccgctggca
    19141 tacagtttgg cggtgtagga ggtttccggg tcggcagatt tgcgttcggc gagcagttgt
    19201 tccagttgat acaggaacag ccactggtga gcggtgtcgc cgaagcagct gctggtgcct
    19261 ttgtggcagg tcgggccgat gggattcgcc agcaccagta acgtgtcgtt gtcgcagtcc
    19321 ggcgcaatat ttactacgtt gaggaaattg cccgacgttt cgcctttggt ccacagtcgc
    19381 tgtttagtgc gcgagaagaa ggtgactttg ccgctttcga tggttttatc taaggcttcc
    19441 gggttcatat agcccagcat taacacttca ccggataccg cgtgctgcac aatcaccggc
    19501 ataagtccgt cggttttttc ccagtccagt tcgcgacgtt gttgttctgt taacatatcc
    19561 tgatctccac gccctgtgtt gccaggtacg c
//
