LOCUS       LC177547               11322 bp    DNA     linear   BCT 15-DEC-2018
DEFINITION  Escherichia coli DNA, O-antigen biosynthesis gene cluster OgN3,
            strain: E1548.
ACCESSION   LC177547
VERSION     LC177547.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., von Mentzer,A., Kikuchi,T. and Thomson,N.R.
  TITLE     An untypeable enterotoxigenic Escherichia coli represents one of
            the dominant types causing human disease
  JOURNAL   Microb Genom 3 (2017) In press
  REMARK    DOI:10.1099/mgen.0.000121
            Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11322)
  AUTHORS   Iguchi,A., von Mentzer,A. and Thomson,N.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2016) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuenkibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..11322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E1548"
                     /db_xref="taxon:562"
                     /note="Escherichia coli O-antigen biosynthesis gene
                     cluster OgN3"
     gene            14..1408
                     /gene="wcaM"
     CDS             14..1408
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAV90427.1"
                     /translation="MPFKKLSRRTFLAASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFILQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     NHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVISNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            1566..2561
                     /gene="gne"
     CDS             1566..2561
                     /gene="gnu"
                     /note="OgN3_1"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAV90428.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNDAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            2804..3697
                     /gene="galF"
     CDS             2804..3697
                     /gene="galF"
                     /note="OgN3_2"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAV90429.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     CDS             3914..4828
                     /note="OgN3_3"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf4"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90430.1"
                     /translation="MQQCLSVTVRNINEFSALVAVKPGAVAWIDGMKNKCPRIAVIMS
                     VYINDKFNEFKEAFDSIINQTYSNFVFFIYADGKINPDIESFLQNYNSQHVIFIRSEK
                     NKGLAYALNKLISVALVNNEFDFIARMDADDISLEDRFQRQIDFFMANPELDVVGGLC
                     QEFGGESAREKCKIYFTDEAIKNNAFKKCPFVHPTVMFKSSVFNNGMRYPENQPLTED
                     MCLWLELISANKKFANIDSVLLKYRINEQTLKRRRGLNKAVTELSTRLYYAKKLKMLS
                     VRNLLYILIHFGVRVLPSNIFKLVHKLN"
     CDS             4830..6596
                     /note="OgN3_4"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf5"
                     /product="hypothetical protein"
                     /protein_id="BAV90431.1"
                     /translation="MNKTVYTYDVWDTILKRHCLPSYTLEVSLYWLLLYLGKPEKHKD
                     VYKKVKALESDFFNRKEEYFIEELIVGILIENNLMNGFCNNSVTAAWEQIYYFVERNC
                     TYKNKEVIELIGSDYGDKLFISDFYTDSSFIKKLLHHHQVSVLDGVTSAEYGVSKHMG
                     SLYEKIELLKQRKWIHTGDNELADIKMAKRAGAQVRLIKSKKKKINKLKIKNDYERLA
                     FIILSFSLFILKTSRKKKCNKIYFFTREGVFFKKNFDLLLNQIPNIFPSITTEILPIS
                     RVASLALKFNESDEFLGFNDALTQYGYGVSTFLSFFHLDDCYSELTTKYHDVNDLLSH
                     RNDPLVKQLIKSIEDKKKRTENFLTSIEFDSEPSIIVDIGWRGSIQDNLMSRKNNYIQ
                     HGCYLGLFDFYPGQSGQNKSSVIFDNNRFRQKWTMKGVAFMETVFNALDGSVVDYVNN
                     KPKRKENYAEIENSKHLIVMQDAIIEEFMNLSIMLKEGKLNINDIERLAIESYKKIVT
                     NPSKEIADFYVGSIQNESFGLNDFIDKEFNISAVDVFRSFFSKSKRNEIRIKLHRNGW
                     RESILKSSKVSLSAKLCSLLIR"
     CDS             6600..7472
                     /note="OgN3_5"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf6"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90432.1"
                     /translation="MNISVIICCYNSENRIVNTLFHLSQCVSLSKGLIDEIILVDNNS
                     SDETVKTAEDFWRNTGEEQIFKIVFESIPGLVHARRRGLDESKNEIVVFCDDDNWLVP
                     SYFTKLEKYFENKQVGAVGGRGIPIANTVIPEWFFENLDAYACSAQTSNMLYGASLAI
                     RSSILKKFYTDALSVKLKGRTKDSLDSGEDNLLCNFVKKQGYTLIPDNTPFCHFMESK
                     RLDVDYLYKLAESFGKATASMKNNSFTTDYVYLTAKMLLAISRIFSKKNKKIYLKREI
                     SFLRGYLRFRKESK"
     gene            7472..8650
                     /gene="wzy"
     CDS             7472..8650
                     /gene="wzy"
                     /note="OgN3_6"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAV90433.1"
                     /translation="MRGGMLVIAWYSIFLLISATGMTGLNKLSTEAILCYIAFLFCFF
                     FGMMLGGVSLPNGMKMLQQEQKIKKRHASAIVLIYCFFIFYSLIALLNIYYFNKVDLS
                     SYRQAFFDGSRDNVKYFYGVFGFYFYYLLSLLVFALVPYTIITNSKKTISLCFFALLL
                     YDIIFLSRTGVYYFILSYIVSSILLKKGIKKIIIVGMVALLFSLIMSYTRDMKSDIIG
                     TLFSSIINYHIAPFVLFDENIISNHLIKYNGAGLASLGIYNIIFYPIDSSIMSSINDF
                     RAQLNEFYDLGVNRYLPYNAYYTSLGGVYIDSGLIGSMMVSFMTGFIIVGIEKSAFKS
                     TKFLVSSVFFTTLCMESIFSPITLNIFAFTIIVYLVVLMVLKYENSDDNTIFKKYRAK
                     "
     CDS             8613..9626
                     /note="OgN3_7"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90434.1"
                     /translation="MIIPSLKNTAPNNIALSICEGARKHGVNIDIYYLDEIVELDIDE
                     SELNINRLNDRTILNSYDIVHSHGLRPDVINCKTSKNVINVSTLHSFILDDLKNKYGI
                     KGYLIAFAWLKLLKRFDSCIAISEATKRFYVKHGLKCHHIYNGIDFPDIELYQDNSRL
                     NNGKINIVSIAHLEKIKGLEQLLYLAAEREEFHVHIIGEGTYRGKLTNIIDKFDLSQR
                     VTLHGYISNASSMLGKFDVYVQPSKSEGFGIAVIEALVNKIPTVCSDIEVFRELFGSG
                     EVEFFKLDDINSLYDAISSALTKTDMSRDASATAISQKFSSEVMSLNYLSWYKKLYEE
                     RNL"
     gene            9607..10785
                     /gene="wzx"
     CDS             9607..10785
                     /gene="wzx"
                     /note="OgN3_8"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAV90435.1"
                     /translation="MKKEIYKYGTIILCYGINFILPILFFKEISLILSPIVLSSFFFV
                     LALLMYCQFIVEYGLQISLLRKLNENKSDLSRLLSTILGLKVILFVLCAVFIYCILLY
                     NNEVIFFILVFILLGNVFSCQFLYQVVDQLHFFYVLNSLVKLIFIPLIFINDNYIYLM
                     ICYSCFNILPNLAALAYFLYKNKVKVVKVPACEIYNLSKECFSYFISNISITLYTNFY
                     QILLGIISPAYLAAYALSDKIIRGIVSGNYVFIQIVQVQYLNDESKFINGTWKTIVSL
                     LLLGGMEFLFIFFGADFFSRYLFPTISLLPIFLKLMSLLIVVILLSNFFAMVYLPCSG
                     NTLFLSRVLIFVSIVSVVVAPILIWLYAGYGAVISAIFSELLVLILCYGLYLKLKHKL
                     "
     CDS             10808..11248
                     /note="OgN3_9"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="hypothetical protein"
                     /protein_id="BAV90436.1"
                     /translation="MNLLIVFCFYISVLRSNKISVFKLITITTVLIVYVFWLIADSFT
                     GVGINDSVYFHMLMPHEGASVENLTYYGFLIVSLVISIFLIAYVSWKKKHNRGLSKNY
                     SFYVLFAIVLVTPFMRNFLGSINNLISEQFSTEDISKEYVINSR"
BASE COUNT     3532 a   1885 c   2305 g   3600 t
ORIGIN
        1 tagaggagaa cgcatgccat ttaaaaaact ctcccgacgc accttcctgg cggcaagctc
       61 ggcgcttgcc ttcctccata cccctttcgc tcgcgcactt cccgcccgac aaagcgttaa
      121 cattaacgac tacaacccac acgactggat cgcctcattt aaacaagcct tcagcgaagg
      181 gcaaacggtc gtcgtgcctg ccggattggt ttgtgacaat atcaacaccg gcatcttcat
      241 tcctgcaggt aaaacgttac acatcttggg aagcctgcgc ggcaacggca gagggcgatt
      301 tatcttacag gacggcagcc aggtgacagg ggaggagggc ggcagtatgc ataacatcac
      361 cctggatgtg cgtggctctg actgcaccat caaagggctg gcgatgagcg gttttggccc
      421 ggtaacgcag atttatatcg gcggcaaaaa caaacgggtc atgcgcaacc tgaccatcga
      481 taatctcacc gttaaccacg ctaattacgc tattttgcgc cagggatttc ataaccagat
      541 aatcggtgcc aacatcacca attgtaagtt cagtgactta caaggcgacg ccattgaatg
      601 gaacgtggca attaacgaca gtgatatttt gatatctgac catgtcatcg agcgcatcaa
      661 ctgtactaac ggcaaaatca actggggcat tggcataggt cttgcgggaa gtacctacga
      721 taacaactac ccggaagacc aggcagtgaa aaactttgtc gtggcgaata tcacgggatc
      781 ggattgtcgg cagttgatcc atgttgaaaa tggcaaacat tttgttatta gtaatatcaa
      841 agcccgcaat atcacgccgg atttcagtaa gaaagcgggc attgataacg ccacggtcgc
      901 tatttacggt tgtgacaatt tcgtgattga taatattgaa atgattaata gcgccgggat
      961 gttaatcggc tatggggtaa ttaaaggcaa atatctctcg ataccgcaaa atttccgagt
     1021 gaataatatt caactggata acacccatct tgcttataaa ttgcgcggca tccaaatctc
     1081 cgccgggaat gctgtctcct ttgtggcgct gactaacatt gagatgaagc gtgcgtcgct
     1141 ggagttacac aacaaaccgc aacatctttt tatgcgtaat atcaaggtga tgcaggaatc
     1201 ctcagttgga ccagcattga gcatgaactt cgacatgcgc aaagacgttc gcggcgtctt
     1261 tatggcgaaa aaagaaacac tcctgtctct tgcaaatgtt catgcggtga atgaaagagg
     1321 gcaaagctcc gtcgatatcg acaggataaa tcaccatatt gttaatgtgg aaaagattaa
     1381 ctttagattg ccggaacgga gggagtagat ttgcgaccat tcataaactt actggatata
     1441 atgttctaaa agattataat cctcacacta actgttaaag ctggggctgg gcagcattga
     1501 ctactcaggg aaggtactca tctttatctt gtgtttctta cgtaacaacc agactaaagt
     1561 gatttatgaa cgataacgtt ttgctcatag gagcttccgg attcgtagga acccgactac
     1621 ttgaaacggc aattgctgac tttaatatca agaacctgga caaacagcag agccactttt
     1681 atccagaaat cacacagatt ggcgatgttc gtgatcaaca ggcactcgac caggcgttag
     1741 ccggttttga cactgttgtg ctactggcag cggaacaccg cgatgacgtc agccctactt
     1801 ctctctatta tgatgtcaac gttcagggta cccgcaatgt gctggcggcc atggaaaaaa
     1861 atggcgttaa aaatatcatc tttaccagtt ccgttgctgt ttatggtttg aacaaacaca
     1921 accctgacga aaaccatcca cacgatcctt tcaaccacta cggcaaaagt aagtggcagg
     1981 cagaggaagt gctgcgtgaa tggtataaca aagcaccaac agaacgttca ttaactatca
     2041 tccgtcctac cgttatcttc ggtgaacgca accgcggtaa cgtctataac ttgctgaaac
     2101 agatcgctgg cggcaagttt atgatggtgg gtgcaggaac taactataag tccatggctt
     2161 atgttggaaa cattgttgag tttatcaagt acaaactgaa gaatgatgcc gcaggttatg
     2221 aggtttataa ctacgttgat aagccagatc tgaacatgaa ccagttggtt gcggaagttg
     2281 aacaaagcct gaacaaaaag atcccttcta tgcacttgcc ttacccacta ggaatgctgg
     2341 gtggatattg ctttgatatc ctgagcaaaa ttacgggcaa aaaatacgca gtcagctctg
     2401 tgcgcgtgaa aaaattctgc gcaacaacac agtttgacgc aacgaaagtg cattcttcag
     2461 gttttgtggc accgtatacg ctgtcgcaag gtctggatcg aactctgcag tatgaattcg
     2521 ttcatgccaa aaaagacgac ataacgtttg tttctgagta atgcagacga gtcagagaaa
     2581 aaaggaccag gttggactac gattattcgg aaagaggtac tgattaaatt atcagtatgc
     2641 ggaattccga gtaatccgag taacccagat gctgatggtg tgtagtgtta tcgtaaggtt
     2701 taatcaaagt tatgcggaac taagctttgg tatatagcca taacgtttgg ctaacctatg
     2761 gcaataattt acgcactatc agccacttag aggtaggaaa agaatgacga atttaaaagc
     2821 agttataccg gtagcaggtc ttgggatgca tatgttgcct gccactaagg cgattcccaa
     2881 agagatgcta ccgatcgtcg acaagccaat gattcagtac attgttgacg agattgtggc
     2941 tgcagggatc aaagaaatcc tcctggtaac tcacgcgtcc aagaacgcag tcgaaaacca
     3001 cttcgacacc tcttatgaat tagaatctct ccttgaacag cgcgtgaagc gtcaactgct
     3061 ggcggaagta cagtctatct gtccgccggg cgtgaccatt atgaacgtgc gtcagggcga
     3121 acctttaggt ttaggccact ccattttgtg tgcacgaccc gccattggtg acaacccatt
     3181 tgtcgtggta ttgccggacg ttgtgatcga cgacgccagt gccgacccgc tgcgctacaa
     3241 ccttgctgcc atgattgcac gtttcaacga aacgggccgc agccaggtgc tggcaaaacg
     3301 tatgccgggt gacctctctg aatactccgt cattcagact aaagagccgc tggatcgtga
     3361 aggtaaagtc agccgtattg ttgaattcat agaaaagccg gatcagccgc agacgctgga
     3421 ctcagacatc atggccgttg gtcgatatgt gctttctgcc gatatttggc cggaacttga
     3481 acgcactcag cctggtgcat gggggcgtat tcagctgact gatgccattg ccgaactggc
     3541 gaaaaaacag tccgttgatg ccatgctgat gactggtgac agctatgact gcggtaaaaa
     3601 aatgggttat atgcaggcgt ttgtgaagta tggactgcgc aacctcaaag aaggggcgaa
     3661 gttccgcaaa ggcattgaga aactgttaag cgaataatga aaatctgacc gaatgtgacg
     3721 gttggcaagg aaactataac agcagtgaag attcgtggcg aaagtaattt gttgcgaata
     3781 ttcctgccgt tattttgtat aaacaatcag aataacaaat agttagcaat aggcttttcg
     3841 tcaaagtttt cctggatttt ccttgtttcc agaacggatt ggtaagacaa ttagcgtttg
     3901 agtttcgagg gagatgcagc aatgtctctc agtaacagta cgcaatatca atgagttcag
     3961 tgcactggta gctgttaagc caggggcggt agcgtggatt gatggaatga aaaataagtg
     4021 cccacgaatt gcagtaataa tgtcggttta tattaatgat aaatttaatg agtttaaaga
     4081 ggcttttgac agtattatta atcaaacata ctctaacttt gttttcttta tttatgccga
     4141 tggcaagata aatccagata ttgaaagttt tttgcaaaat tataattcgc aacatgtgat
     4201 tttcattaga agtgaaaaaa ataagggatt agcttatgct ttgaataaat taatatcagt
     4261 ggcgctagtt aataatgaat ttgattttat tgccagaatg gatgctgatg atataagttt
     4321 agaagataga tttcaacgac aaatagattt ttttatggct aaccctgagt tagatgttgt
     4381 tggtgggttg tgtcaagagt ttggtggtga gtcagcaaga gaaaaatgca agatctattt
     4441 tacagatgaa gccattaaaa acaatgcctt taaaaaatgt cctttcgttc atccaaccgt
     4501 gatgtttaaa tcgtctgtat ttaataacgg gatgcgatac cctgaaaatc agccactgac
     4561 agaagatatg tgcttatggc tcgagttaat atccgcgaat aaaaagtttg caaatattga
     4621 ctccgttctt ctaaagtaca gaattaatga gcagacgcta aaaagaagaa gaggtttgaa
     4681 taaggctgta accgaattat ccacaagatt gtattacgcc aagaaactta aaatgcttag
     4741 cgtacggaat ttgttatata tattaatcca ttttggtgtc agagtccttc cttcaaatat
     4801 atttaaactt gtacataagt taaattaaaa tgaataaaac tgtttataca tatgatgtat
     4861 gggatactat tttaaaacgt cattgtttac catcatatac gttagaggtt tcgttgtact
     4921 ggctactttt gtatcttgga aaacctgaga aacataagga tgtgtataaa aaagtaaaag
     4981 cactggagag tgattttttt aatcgaaaag aagaatattt tattgaagag cttattgttg
     5041 ggatattaat tgaaaataat ttaatgaatg gattttgcaa taactctgtg actgctgctt
     5101 gggaacaaat atattatttc gtagagagaa attgtacata taaaaataaa gaggttatag
     5161 agttaattgg gagtgactat ggggacaaat tatttatttc tgatttttat actgactcca
     5221 gtttcataaa gaaattgcta caccaccacc aagtatcggt tttagatggt gtgacgtcag
     5281 ctgagtatgg cgtcagtaag catatgggtt cattgtatga aaaaatagaa ctattgaagc
     5341 aaaggaaatg gatccatact ggagataatg aattagctga tataaaaatg gcaaagcgtg
     5401 caggtgccca agttagactt ataaaatcca aaaagaagaa aataaataag ttaaaaataa
     5461 aaaatgacta tgagcggtta gcatttatta ttttgtcctt tagcctgttt atattaaaaa
     5521 cttccagaaa gaaaaagtgc aataagatct acttttttac aagggagggg gtttttttca
     5581 aaaaaaactt tgatcttcta ctaaaccaaa tacctaacat attcccatct ataactacgg
     5641 aaatattgcc aatcagtcga gtagccagct tagcattgaa gtttaatgag tctgatgaat
     5701 ttttaggatt taatgatgca ttaacccagt atggctatgg agtgtctacg tttcttagtt
     5761 tctttcatct tgatgattgt tactccgagc tcacaacaaa gtatcatgat gtaaatgatt
     5821 tattatctca cagaaatgat cctttggtaa aacaactaat aaaaagtata gaggataaaa
     5881 aaaaacgaac agaaaatttt cttacatcca ttgagttcga ttctgagcca agtatcatcg
     5941 tggatattgg ttggcggggt agcatccaag ataatttaat gtcgcggaaa aataattata
     6001 ttcaacatgg atgttatctc ggattgtttg atttttaccc aggccagtca ggtcagaata
     6061 aatcatctgt aatttttgat aacaacagat ttagacaaaa atggaccatg aaaggtgtag
     6121 cttttatgga gacggttttc aatgctttgg acggaagtgt tgtcgattat gtaaacaata
     6181 aaccgaaaag aaaagaaaat tacgctgaga tagaaaattc aaaacattta atagttatgc
     6241 aagatgctat cattgaggaa tttatgaatc tatctattat gttaaaagag ggaaagttaa
     6301 atatcaatga tattgaaaga ttagctattg aaagttacaa aaaaatagta actaacccat
     6361 ctaaagaaat agctgatttt tatgttggca gtatccaaaa tgaaagcttc ggtttgaatg
     6421 attttatcga caaagaattt aacattagcg cagtagatgt tttccgaagt tttttttcta
     6481 aaagtaaaag aaatgaaata agaataaaat tgcatagaaa tggatggcgt gagtcgatac
     6541 ttaaatcctc taaagtatcg ctgagcgcaa aattatgttc acttttaatt agataaaata
     6601 tgaatatttc agtaataatt tgttgctata actcagaaaa tagaatcgta aacacccttt
     6661 ttcatctttc acaatgtgta tctctatcca aggggttaat tgacgagatt attcttgttg
     6721 acaataactc atctgatgaa actgttaaaa cagctgagga tttttggcgt aatacggggg
     6781 aggagcaaat ctttaaaatt gtttttgaat cgattccggg ccttgttcat gctagacggc
     6841 gagggctgga cgagtccaaa aatgagattg ttgttttttg tgatgatgat aattggctgg
     6901 taccatctta ttttacaaaa ctagagaaat attttgaaaa caaacaagta ggcgctgtcg
     6961 ggggacgcgg tattcctatt gctaataccg ttattcctga gtggtttttt gagaatctgg
     7021 atgcatatgc atgttctgca caaacttcta acatgttgta tggtgctagc ttggctattc
     7081 ggtctagtat cttaaaaaaa ttttatacgg atgctttatc agtaaagcta aagggtagaa
     7141 caaaagacag tctggattct ggggaagata acttattgtg taattttgtc aaaaaacaag
     7201 gatacactct gatacctgac aatacaccat tttgccattt catggaaagc aagcgattgg
     7261 atgtcgatta tctatataaa ctggctgaat catttggaaa agcaacggca tcaatgaaaa
     7321 acaattcctt caccaccgat tatgtatatt tgacagcaaa aatgttatta gctatatcta
     7381 ggatcttctc taaaaaaaat aaaaagatat atttaaaacg tgagatttcc tttctcagag
     7441 gttatttgag gtttagaaag gaatcgaagt aatgagaggg ggaatgcttg taattgcgtg
     7501 gtattctatt tttctgttga tttcagctac gggcatgacc ggtctaaata agctgagtac
     7561 tgaagctatt ctgtgctata ttgcattttt attctgtttt ttctttggca tgatgttagg
     7621 tggtgtttcg ctcccaaacg gaatgaaaat gttgcaacag gagcaaaaga taaagaaaag
     7681 gcatgcttct gccatagttc taatttattg cttttttatt ttttattctt taattgctct
     7741 tttaaatata tattatttca ataaggttga tctatcatct tatagacaag cattttttga
     7801 tggttcaaga gataatgtta aatacttcta tggcgtgttt ggcttttact tctattattt
     7861 acttagcctg ttagtattcg ccttagttcc ttatactatt ataacgaaca gcaaaaagac
     7921 tatttcatta tgtttctttg ccttgctgct atacgatatt attttccttt caagaactgg
     7981 tgtttattac tttatattat cttatatagt atcgagtatt cttctcaaga aaggaatcaa
     8041 gaaaattata attgtaggga tggttgcctt attattctca ttgataatga gttacacaag
     8101 ggatatgaag agtgacatca ttggtacatt atttagctca attataaatt atcatatagc
     8161 tccgtttgtc ttgtttgatg aaaatataat atcaaatcat ttgattaaat ataatggggc
     8221 tggcttggca tcgttgggga tatataatat cattttttat cccattgatt cgtctatcat
     8281 gtcatcaata aacgatttta gagcacagtt gaatgaattc tatgatttgg gagtaaatcg
     8341 ttatttgcca tataatgcct attatacttc tttgggtggg gtatatatag atagcggcct
     8401 gattggtagc atgatggtta gttttatgac tggttttatt attgtcggaa tcgagaagag
     8461 tgcttttaaa tcaactaagt tcttagtctc ttctgtgttt tttactacat tatgtatgga
     8521 atctatattt tccccaataa cactaaatat ttttgccttt acaataattg tctacttagt
     8581 tgttttgatg gtccttaaat atgaaaatag cgatgataat accatcttta aaaaataccg
     8641 cgccaaataa tatagccctt tcaatatgtg aaggggcaag gaagcatggt gtcaatatag
     8701 atatttatta tcttgatgaa attgtagagt tggatataga cgagtcagaa ctgaatataa
     8761 ataggctaaa tgatagaacc attttaaaca gttatgatat tgttcactct catggcttaa
     8821 gacccgatgt tattaattgt aagaccagca aaaatgtcat taatgtaagt acgcttcata
     8881 gttttatttt agatgatctt aagaataaat atggaataaa aggatatctg atcgcttttg
     8941 cttggcttaa attattaaag cgatttgatt catgtattgc aattagtgag gcgactaaac
     9001 ggttttatgt taaacatggt cttaaatgcc accatatata taatggaatc gactttcctg
     9061 acatcgaatt atatcaagat aactcaaggc taaataacgg caaaataaat atagtatcga
     9121 tcgcgcatct ggaaaaaata aaaggcttag agcaactcct atatctagcc gcggagcgag
     9181 aagagttcca tgttcatata attggtgaag gaacttaccg tgggaaatta acaaatatta
     9241 ttgataagtt tgatttatct cagcgtgtaa ctttgcatgg atatatatca aatgcgagtt
     9301 ctatgttggg gaaatttgat gtttatgtgc aaccatctaa aagtgagggg tttggcattg
     9361 cagtcattga agctcttgta aataaaatcc caacagtttg ctcagacatt gaagtgttca
     9421 gagagttatt tggctcagga gaagttgaat ttttcaagtt agacgatatt aattcacttt
     9481 acgatgcaat ttcaagtgca ttaaccaaga cagatatgtc tcgagatgca agtgccacag
     9541 cgataagcca aaaattttct tcagaagtga tgtcattgaa ctatttaagt tggtataaaa
     9601 aattatatga agaaagaaat ttataaatat ggcacaataa tattatgcta tggaattaac
     9661 tttatactac ctattctttt ctttaaagaa ataagtttaa tattatctcc aatagtatta
     9721 tcatcatttt tctttgtttt ggcattgcta atgtattgtc aattcattgt tgaatacggg
     9781 ttgcaaatat cattattaag aaagttaaat gaaaataaga gtgatttaag tcggctttta
     9841 tctactattc ttggactaaa agtaatacta ttcgtcctat gcgcagtatt catttattgt
     9901 attttgctat ataataatga agttatattt tttatacttg ttttcattct gctaggaaac
     9961 gtattctcat gccagttcct ctatcaagta gttgatcagc tccatttttt ttatgtatta
    10021 aattcgcttg ttaagctaat tttcataccc ctcattttta tcaatgataa ttatatctat
    10081 ctgatgatat gttatagttg tttcaatatc ctacctaact tagctgcact tgcttatttt
    10141 ttatataaga ataaagttaa ggttgtaaaa gttcctgctt gtgaaattta taatttatct
    10201 aaggaatgtt ttagctattt tatctcgaat atttcgataa cattatacac taatttttat
    10261 cagatcttat taggcattat atcgcccgct tatctggccg cgtatgcatt aagtgacaaa
    10321 attattaggg ggatcgtgtc agggaattat gtgtttatac agatagtaca agtacaatac
    10381 ttaaatgatg aatcaaagtt catcaatggt acatggaaaa cgattgtttc attattatta
    10441 ctggggggga tggagttttt atttattttc tttggagcgg attttttctc tcgttattta
    10501 ttccctacaa tatctttact gccaattttt ctaaaactaa tgtcattgct aattgttgtt
    10561 atcttgttga gtaacttttt tgcaatggtt tatcttccgt gttcaggcaa tactttattc
    10621 ctctcacgtg ttctgatttt tgtttctatt gtgtccgtag tagttgcccc gatactaata
    10681 tggctctatg caggttatgg tgcagtcatt tccgctatat tttcagagtt attagttctg
    10741 attttatgct atggtttata tttgaaatta aaacacaaac tctaactatt aacgtagaat
    10801 aattaaaatg aatttattga ttgttttttg cttttatatt tcggttctgc gtagtaataa
    10861 aataagtgta tttaaattaa tcactatcac aactgtcttg attgtatatg tattctggct
    10921 aatagccgac agttttactg gcgttgggat aaatgattca gtttatttcc atatgttaat
    10981 gccgcatgag ggggcatcgg ttgaaaattt aacatattat ggatttctga ttgtaagcct
    11041 tgttatttca attttcttga ttgcttatgt ttcttggaaa aagaagcata atagaggatt
    11101 aagtaaaaat tactcttttt acgtactctt cgctattgta ttagttactc catttatgcg
    11161 taatttcctg gggtcaatta acaatttaat ttccgaacag ttttctactg aagatataag
    11221 taaagagtat gtaatcaata gtaggtgatg ctgccaactt actgatttag tgtatgatgg
    11281 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gt
//
