LOCUS       LC125932               19501 bp    DNA     linear   BCT 04-JUN-2016
DEFINITION  Escherichia coli DNA, O-antigen biosynthesis gene cluster, strain:
            100998.
ACCESSION   LC125932
VERSION     LC125932.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Seto,K., Nishii,H., Ohnishi,M., Mekata,H.,
            Ogura,Y. and Hayashi,T.
  TITLE     Six Novel O Genotypes from Shiga Toxin-Producing Escherichia coli
  JOURNAL   Front Microbiol 7, 765 (2016)
   PUBMED   27242776
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19501)
  AUTHORS   Iguchi,A., Iyoda,S. and Seto,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2016) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuenkibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..19501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="100998"
                     /db_xref="taxon:562"
                     /note="O genotype:OgN31; serogroup:O39"
     gene            <1..906
                     /gene="wcaM"
     CDS             <1..906
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAU71632.1"
                     /translation="NYAILRQGFHNQIIGGNITNCKFSDLQGDAIEWNVAINDRDILI
                     SDHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVE
                     NGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVI
                     KGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEVKRASLELHNK
                     PQHLFMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSS
                     VDIDRINHHIVNVEKINFRLPERRE"
     gene            1069..1974
                     /gene="galF"
     CDS             1069..1974
                     /gene="galF"
                     /note="OgN31_1"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAU71633.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            2347..3423
                     /gene="rmlB"
     CDS             2347..3423
                     /gene="rmlB"
                     /note="OgN31_2"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAU71634.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSIVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LNNNDWCRRVQDGSYQREHLGLVK"
     gene            3420..4286
                     /gene="rmlA"
     CDS             3420..4286
                     /gene="rmlA"
                     /note="OgN31_3"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAU71635.1"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIRDILIITTPEDQHGFKRLLGDGSSLGINLQYEIQNSPDGLAQAFIIGEKFIANDSV
                     CLVLGDNIFYGQGFSPKLKEAFIRAKEKATIFGYQVKDPERFGVVEFDNQRNVLSIEE
                     KPSNPKSNYAVTGLYFYTNEVVEYAKLVKPSSRGELEITSINQMYLENGKLAVEMLGR
                     GFAWLDTGTHESLLQASQFVETIETRQGFKVACLEEIAFANGWLTKKELLIRSSLMSK
                     NSYGQYLLQLIK"
     gene            4293..5720
                     /gene="wzx"
     CDS             4293..5720
                     /gene="wzx"
                     /note="OgN31_4"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAU71636.1"
                     /translation="MAELKYKTINSLKWSALERIFSQGIQLILMLVLARLLGPKAFGL
                     IGMLAIFIAIGQVFIDSGFSSALIRKNDRTHIDYATAFYFNITVAILFYVVLFFSAPL
                     IAKFYHQPELELLSKIIGLSLITNSFCVVPRTILTIAMDFKSQAKVSVLSVVLGGACA
                     LFLAWKGFGVWSLVAQTLTVSMCNALLLNIITKWIPGLFFSKKSFLYLFGFGGKLLIS
                     GLLDTIYNNMYQIIIGKKFSAANVGQYTQANQISSIPAMTLTNIIQRVTYPMFSHMQD
                     DEDKLKKNYLLTLKLAALFIFPIMAGIGIIAQPLFFIILGPQWIDASRLIIILSFAYM
                     IYPIHAVNLNILQVKGRSDLFLQLEIMKKLIGVIVILITMNYGIYGMCFGLVFHSYLC
                     LIVNTYYTKKMTKLTLKQQMIELGPVWIITIISCTIAYIGALHISDDALLQVLLMVVI
                     AVALYIGLIFLLQRNLLKLALSIRK"
     gene            5732..6835
                     /gene="vioA"
     CDS             5732..6835
                     /gene="vioA"
                     /note="OgN31_5"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-viosamine synthetase"
                     /protein_id="BAU71637.1"
                     /translation="MKKIPVTQPFLPDLNEFIPYLEKIWENKWLTNNGPFHQQLEKEL
                     CKYLGVEYVSIFNNATIALITALQSLRITGEVITTPYSFVATSHSILWNGLKPVFVDI
                     DPETFNIDPKKIEEAITPETTAIMPVHCYSNPCEVEAIQEIADNYGLRVIYDAAHAFG
                     VNYKGRSLLSYGDMSVLSFHATKVFNTFEGGAIICQDAKLKQRIDRLKNFGIADELTV
                     TAPGINGKMSELNAAFGLVQLKYIDTAIAQRKSIDFLYRSELSKINGITIPKPSSNSN
                     SNYSYFPVLVESNFPLSRDELYEELKRNNILSRRYFYPLISNMPMYKSGKTSSVHHLP
                     MANDVSNKVLCLPIFNELSEEDCCGIIEIIKNC"
     CDS             6887..7954
                     /note="OgN31_6"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="glycosyltransferase"
                     /protein_id="BAU71638.1"
                     /translation="MSKEVSVLLLLISNLTNKIVCQFYHYGENVEEVVVSICCITFNQ
                     ELYISTAIESFLAQKTDFPFEIIISDDKSNDNTVGIIQEYVEKYPDLIKLNVNRENIG
                     ANANLLQAIKRARGKYIALCEGDDYWIDELKLQLQYETMLKNPQSLFCFHSCRLHKNS
                     KLEEKVYFDKGGDVRLFGVDDILQDLKQFAPTASYMFARHLDQLLPDWFSAAPVGDLF
                     LELYGMKNGGGVYIPRPMCAYRISSLGSWTETTQSKTNIHRKRHLEIIRFLKKSQIDF
                     PLYKSAFDKKISHIYLSLAIRAVKNKDYIDFRRFIEQSICYNNEISISQKICNKLKSA
                     PLMLYYLLCLKSVFSINQKKY"
     gene            7976..9184
                     /gene="wzy"
     CDS             7976..9184
                     /gene="wzy"
                     /note="OgN31_7"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAU71639.1"
                     /translation="MTRKYLAVLFAFFNISCAFYFGMLNSLGGDFKYEFTADVSLLLL
                     STIIALLTFFFIQVVVFSFFEKIRMDKKILYNNNISLDFIIIIITSFAIFFSLYYKVG
                     VLGINKDIIEDAPKMVFYFNSIFQPSYLILVYLFYRYDSVRLIYYANFILYILLLLVS
                     GQTGQLLILFCLYIMRKRNNFNLLRLFVLSLMGISFYPIIRIFKDAIVQSVNGNESLV
                     GALVSVYSNLDSELYFRYLFITLERFQIVSNIHYIIENGRVLYSGFLHTGSSDTFFSL
                     YWIFSAISKLLGYHTAHIISAQDFMAININGIPTWSSQIGVLGYFYFYQVYAVLIIMF
                     MFFILFISITLSKYLSRNKEIINLTWFLSLMLLCHGWFIPFINYVQCLAVFVALISIL
                     KTRTQKFVRN"
     CDS             9197..10363
                     /note="OgN31_8"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="glycosyltransferase"
                     /protein_id="BAU71640.1"
                     /translation="MKRKVVVFRSKLLPFSETFIKEQVLSYSKWEPVLMGHCLLKNRV
                     NLDDITNKIVFDYNDSGVSCFKRKVYKFFNVCPIKDKKIIAQQRADLIHIHFGVDAVV
                     NWSTFKQFKKPIVITLHGYDINIKKEWWASGNEGRHLKRYPERLLEISKYNNVYFIAV
                     SKAIKERAIAFGIPPKKISVSYIGIDTFSFKNSMTPFNERRQILFVGRLVEKKGCEIL
                     INAYAKLHRQHYGYPLKIIGDGPLKHQLETYAKKLNLNVQFTGVLSKREIIAELNKTA
                     IFCLPSIHASSGDAEGFGLVLLEAAACGVPVITSAFGGAEEGVIHGLTGFSFPEKDTN
                     RLSYYLNLLLSDPNLMESMGKAGQQFVADNFDLKKCTEELEVIYNSIMEQFFEK"
     CDS             10375..11211
                     /note="OgN31_9"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="glycosyltransferase"
                     /protein_id="BAU71641.1"
                     /translation="MLNKSKIDVVVPSYNRLDYLLQSIKSIKKQSYTANQIIVVDDDS
                     EFSEKDFWSGLKRENICTDRILFYKKEKNLGACHSRNLGVQLSKSEYVAFLDDDDLWE
                     INHLEGLINCFQNDDIVLAYSGKLIKNFTTGKERKSLNVIPDEQQFLSLLKCNYPGST
                     SSIMVRKKSFFAAGGFDETLPAIQDYDFYLRIVKEGRITTSNIFTLIYRVDTPVKITN
                     QLYKARKAYYLIIEKLVPQHRKVLAKTISIQNIKKAVINKKMNYIILFSFDYIKNALR
                     KG"
     CDS             11214..12320
                     /note="OgN31_10"
                     /codon_start=1
                     /transl_table=11
                     /gene="glf"
                     /product="putative UDP-galactopyranose mutase"
                     /protein_id="BAU71642.1"
                     /translation="MKYDYLIVGSGLYGSVFAYEMKKRGFTVKIIEKREHVGGNVYCK
                     NIDGINVHVYGAHIFHTNDKKIWDYVNELVEFNRFTNSPLAFSKGKLFNLPFNMNTFY
                     QLWGVTSPDEAKAIIEHQSKQPNVIEPQNLEEQAISLVGKDIFNTLIKEYTEKQWGRN
                     CKELPPFIIKRLPVRFTFDNNYFNDKYQGIPIGGYNKLIESLLDGVEVETNTDFFENK
                     EYWLKSANKIVYTGPIDRFFGFEFGELAYRTLEFEHEEINKSNFQGNAVINYIDSQYP
                     YTRIIEHKHFEFGCQEKTIITKEYPKEYSKGDEPYYPVNDSYNNRLFSYYKQKAKNLD
                     NVIFGGRLAEYKYYDMHQVIASALTAVEKELCTE"
     CDS             12308..13222
                     /note="OgN31_11"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf12"
                     /product="glycosyltransferase"
                     /protein_id="BAU71643.1"
                     /translation="MYRMKKVIAVVVTFKRKSLLEDVIKGLAEQSYPLSEIIIIDNHS
                     CDGTDILVDNLRKVYATSKISYYDTGDNLGGAGGFAYGFEVAQKSDFEYLWLMDDDLL
                     PESDCLENLLATGCDGIIQPIRFNKDGSCAEISPIEYDLDSVFIINPKKKTVKDIIEL
                     KNVDIPVPIAGVPFEGPLISKSVVDKIGKPESKFFIFNDDLDYSIRARNAGFKILCQP
                     KATAIRLLVNNQSNDLKSWKGYFMLRNHFYILRTHGKNFFVKSRPIILTLGYAALSIF
                     RGDLDVALKVCRAYRDSYYLSNNDRFKP"
     gene            13516..14922
                     /gene="gnd"
     CDS             13516..14922
                     /gene="gnd"
                     /note="OgN31_12"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAU71644.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLTGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            15144..16337
                     /gene="ugd"
     CDS             15144..16337
                     /gene="ugd"
                     /note="OgN31_13"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAU71645.1"
                     /translation="MFNRKKSELMKITISGTGYVGLSNGILIAQNHEVVALDIVQAKV
                     DMLNKKQSPIVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFN
                     TSSVEAVIRDVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYD
                     NLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVA
                     YFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQS
                     VPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRI
                     KAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRD
                     LFGSD"
     gene            16479..17453
                     /gene="wzz"
     CDS             16479..17453
                     /gene="wzz"
                     /note="OgN31_14"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAU71646.1"
                     /translation="MMRVENNNVSGQNHDPEQIDLIDLLAQLWRGKMTIIISVIVAIV
                     LAIGYLYVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAPKVSEIQSMLIGRFST
                     TFSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQV
                     NEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQI
                     QQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIH
                     AYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGVVLGRNLLYGYK"
     CDS             complement(17734..18327)
                     /note="OgN31_15"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf16"
                     /product="putative galactoside O-acetyltransferase"
                     /protein_id="BAU71647.1"
                     /translation="MSLKTFDIGYYDTEHLIKCGFKHIGENVLIAKNCNIVGIENISI
                     GSNVRIDAFTTIIANNGGYLNLGSYIHIGGYSAIFAGSGVTMMDFSGISQGVKIYSKS
                     DDYSGKFMTNPCVPSQYTGVIRGEVTLEKHVIVGSQSIIMPNITLKEGTAVGANSLIT
                     KSTEEWSIMFGSPAKKIKSRRKNILKMEEELQNNKHI"
     gene            complement(18582..19193)
                     /gene="hisI"
     CDS             complement(18582..19193)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAU71648.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGNVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     5971 a   3415 c   4028 g   6087 t
ORIGIN
        1 aattacgcca tcttacgcca gggatttcat aaccagatta tcggtggcaa catcaccaac
       61 tgtaagttca gcgacttaca gggggacgct attgaatgga acgtggcgat taacgaccgt
      121 gatattttga tatctgacca tgtcatcgag cgcatcaact gtactaacgg caaaatcaac
      181 tggggcatcg gcataggcct tgcggggagt acttatgata ataactaccc ggaagaccag
      241 gcagtgaaaa actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat
      301 gttgaaaatg gtaaacattt tgttattcgt aatatcaaag cccgcaatat cacgccggat
      361 ttcagtaaga aagcaggtat tgataacgcc acagtcgcta tttacggttg tgacaatttc
      421 gtgattgata atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt
      481 aaaggcaaat atctctcgat accgcaaaat ttccgagtga ataatattca actggataat
      541 acccaccttg cttacaaatt gcgcggtatc caaatctctg ccgggaatgc cgtctccttt
      601 gtggcactga ctaacattga ggtgaagcgt gcgtcgctgg agttacacaa caaaccgcaa
      661 catcttttta tgcgtaatat caaagtgatg caggaatcct cagttggacc cgcattgagc
      721 atgaacttcg acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg
      781 ctgtcacttg caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac
      841 aggattaatc accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg
      901 gagtagattt gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca
      961 atccacaacg aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat
     1021 gctggcgaaa aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata
     1081 atgacgaatt taaaagcagt tattcctgta gcgggtcttg ggatgcatat gttgcctgcc
     1141 actaaggcga ttcccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatt
     1201 gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag
     1261 aacgcggtcg aaaaccactt cgacacctct tatgaattag aatctcttct tgagcagcgc
     1321 gtgaagcgtc aactactggc ggaagtgcaa tctatctgcc cgccgggcgt gactattatg
     1381 aacgtgcgtc agggcgaacc tttaggtttg ggccactcca ttttatgtgc acgacctgcc
     1441 attggtgaca atccatttgt cgtggtgctg ccagacgttg tgatcgacga cgccagtgcc
     1501 gatccgctgc gctacaacct tgctgccatg attgcgcgct tcaacgaaac gggccgcagc
     1561 caggtgctgg caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagaccaaa
     1621 gaaccgctgg atcgtgaagg caaagtcagc cgcattgttg aatttatcga aaaaccggat
     1681 cagccgcaga cgctggactc agatatcatg gccgtgggcc gttatgtgct ttctgccgat
     1741 atctggccgg aacttgaacg cactcagcct ggtgcatggg ggcgtattca gctgactgat
     1801 gccattgccg aactggcgaa aaaacagtcc gttgatgcca tgctgatgac tggtgacagt
     1861 tacgactgcg gcaaaaaaat gggctatatg caggcgtttg tgaagtatgg actacgcaac
     1921 ctgaaagaag gggcgaagtt ccgcaaaggg attgagaagc tgttaagcga ataatgaaaa
     1981 tctgaccgga tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcgaaa
     2041 gtaatttgtt gcgaatattc ctgccgttgt tttatataaa caatcagaat aacaacgagt
     2101 tagcaatagg attttagtca aagttttcca ggattttcct tgtttccaga gcggattggt
     2161 aagacaatta gcgtttgaat ttttcgggtt tagcgcgagt gggtaacgct cgtcacatcg
     2221 taggcatgca tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt gcattaatac
     2281 ctctattaat caaactgaga gccgcttatt tcacagcatg ctctgaagta atatggaata
     2341 aattaagtga aaatacttgt tactggtggc gcaggattta ttggttctgc tgtagttcgt
     2401 cacattataa ataatacgca ggatagtatt gttaatgtcg ataaattaac gtacgccgga
     2461 aacctggaat cacttgctga tgtttctgat tctgaacgct atgtttttga acatgcggat
     2521 atttgcgatg cagctgcaat ggcacggatt tttgctcagc atcagccgga tgcagtgatg
     2581 cacctggctg ctgaaagcca tgttgaccgt tcaattacag gccctgcggc atttattgaa
     2641 accaatattg ttggtactta tgtccttttg gaagccgctc gcaattactg gtctgctctt
     2701 gatagcgaca agaaaaatag cttccgtttt catcatattt ctactgacga agtctatggt
     2761 gatttgcctc atccagatga agtaaataat aacgaagaat tacccttatt tactgagacg
     2821 acagcttacg cgccaagcag cccttattcc gcatcaaaag cttccagcga tcatttagtc
     2881 cgcgcgtgga aacgaaccta tggtttaccg accattgtga ctaactgctc taacaattat
     2941 ggtccttatc atttcccgga aaaattgatt ccattggtta ttctgaatgc tctggaaggt
     3001 aaagcattac ctatttatgg caaaggggat caaattcgcg attggttgta tgtcgaggat
     3061 catgctcgag cgttatatac agtcgttact gaaggtaaag caggcgaaac ctataatatt
     3121 ggcggacaca acgaaaagaa aaacatcgat gtagtgctta ctatttgtga tttgctggat
     3181 gagattgtac cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgaccgcccg
     3241 ggacacgatc gccgttatgc gattgatgct gagaagatta gccgcgaatt gggctggaaa
     3301 ccgcaggaaa cgtttgagag cgggattcgg aagacagtgg aatggtattt gaataataat
     3361 gattggtgcc gacgagttca agatggctct tatcaacgtg agcatttggg gttagttaaa
     3421 tgaaaggaat tattttagcc ggtggttctg ggacaaggct ttatccaatt acaatggggg
     3481 tttcaaagca gctattgccc atttatgata aaccgatgat ttattatcct ttgtcagtgc
     3541 tgatgttggc agggataaga gatattttaa taataactac gccagaggat caacatggtt
     3601 tcaaacgtct acttggtgat ggttcatcgc ttgggataaa tttacagtat gaaatacaaa
     3661 attctcctga tggccttgcg caagctttta tcataggaga gaaatttatt gccaatgatt
     3721 ctgtctgctt agttctgggt gataatatat tctatgggca aggtttttca ccaaagctaa
     3781 aagaggcatt catacgggct aaagaaaaag ctacgatatt tggttatcag gtaaaagatc
     3841 ctgaacgatt tggtgtagta gaatttgaca accaacgaaa tgttttgtca atagaagaga
     3901 aaccgagcaa tcccaaaagt aactatgcag tgacaggttt atatttttat actaacgaag
     3961 tagtagaata cgctaaactt gtgaagccct cttctcgagg agaactcgaa atcacatcga
     4021 ttaatcagat gtatctagag aatggaaagt tggctgtaga aatgctaggt cgtggtttcg
     4081 cttggcttga tacaggtacc catgaaagtt tgcttcaagc ttcccaattt gttgaaacga
     4141 tcgaaactcg tcagggattt aaagtagcat gtttagaaga aatagcattt gctaatggtt
     4201 ggttgacaaa aaaagaatta ttaattcgct caagcttgat gtcaaaaaat agctatgggc
     4261 aatatttatt gcaattaatc aagtgaaata tcgtggcaga actaaaatac aaaacaataa
     4321 atagcttaaa atggagtgca ttggaaagga tattttcaca aggtattcaa ttaatattaa
     4381 tgcttgtttt ggcaagatta cttggaccca aggcatttgg tttaattgga atgcttgcaa
     4441 tatttatcgc aattgggcag gtatttattg atagtggttt tagttctgcc ttaataagaa
     4501 aaaatgatcg tacacatatt gattatgcta cagcatttta tttcaatatt acagtggcaa
     4561 tcttatttta tgttgtccta ttcttttcag caccattaat tgcaaaattt tatcatcaac
     4621 cggaacttga acttctatct aaaataatag ggttatcatt gataactaat agcttttgcg
     4681 ttgtgccgcg aacaattttg actattgcca tggattttaa atctcaggca aaagtatctg
     4741 ttttgtcggt agtgttaggg ggggcatgtg ctctatttct ggcatggaag ggctttggtg
     4801 tttggtcttt agtagctcaa acgttgactg tatcaatgtg taatgctcta ttattaaata
     4861 ttataaccaa atggatacct ggattatttt tttcaaaaaa atcatttcta tatctttttg
     4921 ggtttggtgg gaaattatta atatcaggtc tacttgatac aatatataac aatatgtatc
     4981 aaataataat tggcaagaaa tttagtgccg caaacgtggg gcagtatact caagcgaatc
     5041 agatatccag tattcctgca atgacactga cgaatataat acagagggta acgtacccaa
     5101 tgttcagtca tatgcaagat gatgaagata aattgaaaaa aaattatttg ttgacactga
     5161 aattggcggc attattcatt tttccaatta tggccggtat tgggattatc gcacagccac
     5221 tcttttttat tattttaggg cctcaatgga ttgatgcatc taggctgatt ataatactat
     5281 cttttgctta tatgatttat cccattcatg ctgtgaattt aaatatttta caagtaaaag
     5341 gtagaagcga cttattcctc caacttgaga taatgaagaa gttaataggt gtcattgtta
     5401 ttttgataac aatgaattat gggatttatg gcatgtgctt tgggttagta tttcattcct
     5461 atctttgcct gattgttaat acgtactata caaaaaaaat gacaaaacta actcttaagc
     5521 agcaaatgat tgaactcggt ccagtttgga ttataactat tatatcttgt accattgcat
     5581 atattggagc attgcatata agtgatgatg cactattaca agtgctattg atggtcgtaa
     5641 tagcagtggc attatatatt ggattaatat ttctactaca aagaaattta ttgaaattag
     5701 ccttatcaat tagaaaatag agtatattta aatgaaaaaa atcccagtaa cacaaccttt
     5761 tttaccggat ttaaatgaat ttatcccgta tcttgaaaag atttgggaaa ataaatggtt
     5821 gacaaataat ggtccgttcc atcaacaact cgaaaaagaa ctgtgcaagt atttaggagt
     5881 tgaatacgtt tctatattta ataatgcaac gattgcactg attacagcat tacaatcttt
     5941 gagaattaca ggcgaagtta taacaacacc atattctttt gttgcaacaa gtcactctat
     6001 tttatggaat ggattaaaac cggtttttgt ggatattgat cctgaaacat tcaatattga
     6061 tcctaaaaaa attgaagagg caataacacc ggaaacaaca gccatcatgc ccgtgcattg
     6121 ctattctaat ccttgtgaag tagaggctat ccaggagatt gcagataatt acggcttaag
     6181 agttatttat gatgctgctc atgcatttgg agtaaattat aaaggtcgga gcttattaag
     6241 ctatggtgat atgtcggtct tgagttttca tgcaacaaaa gtgtttaata cttttgaagg
     6301 tggtgcgatt atctgtcagg atgcaaaact aaagcagcgt attgataggt taaagaattt
     6361 cgggatagca gatgagttaa ctgttacagc tcctggcatt aatgggaaaa tgagtgagct
     6421 aaatgctgca tttggtttag ttcaattaaa atatatagat acagcaattg ctcaacgtaa
     6481 atcaattgat tttctttatc gcagtgagtt atcgaaaata aatggcataa caataccgaa
     6541 gccatcatct aattcaaata gtaattactc atattttcct gttttggttg aaagcaactt
     6601 tccgctaagt cgagatgagc tctatgagga attaaaacgg aataatattc tttctcggcg
     6661 ttatttttat ccgctcataa gtaatatgcc tatgtacaaa agcgggaaaa catcttctgt
     6721 acatcatttg ccgatggcaa atgatgtgtc aaataaagtt ttatgtttgc ctatttttaa
     6781 cgagttaagc gaggaagatt gttgtggtat aattgaaata ataaagaatt gctgacattt
     6841 aatataataa atgtaatcat ttaatgacta atatatcgtt ggtcatttga gcaaagaagt
     6901 gtctgtttta ttgcttttaa tcagtaattt gacaaataag attgtatgtc aattttatca
     6961 ttatggtgag aatgtggaag aagtggttgt aagcatatgc tgtattacat ttaatcaaga
     7021 actttatatt tcaactgcca ttgaaagctt tttagcacaa aaaacagatt ttccatttga
     7081 aattataatt tctgatgata aaagtaatga taatacagta ggtattatac aagagtatgt
     7141 agaaaaatat cctgacttaa ttaagttaaa tgttaacagg gaaaatattg gagcgaatgc
     7201 aaacttgctt caagccataa aaagagcaag ggggaaatat attgctttat gtgaaggaga
     7261 tgattattgg atcgatgaac tgaagttgca gttacagtat gaaacaatgt taaaaaaccc
     7321 acaatcatta ttttgctttc actcttgtcg ccttcataaa aatagtaaac tggaggaaaa
     7381 agtttacttt gataaggggg gggatgtccg gctttttggt gtagatgata ttttgcagga
     7441 cttaaaacaa tttgccccta ccgcttcata tatgtttgcc cgacatttag atcagctgtt
     7501 acctgattgg ttttcagctg cccctgtggg cgatttattt cttgaattat acggtatgaa
     7561 aaatggagga ggtgtatata ttccgcgacc tatgtgcgca tacaggattt catcgcttgg
     7621 ctcatggact gaaacgacac aaagcaaaac aaatatacat agaaaaaggc atttagaaat
     7681 aataagattt ttaaaaaaat cacaaataga tttccctctg tataagtccg cattcgataa
     7741 aaaaatctca catatatatt tatcgttggc cataagagct gtaaaaaata aagattacat
     7801 tgattttaga cgattcattg agcaatccat ttgctataat aatgaaatat caatatcaca
     7861 aaaaatatgc aataagctaa agtctgcgcc tttaatgtta tattatttgt tatgtctgaa
     7921 aagtgtgttt agtataaatc agaaaaaata ttaataatta attaaaagta aatatatgac
     7981 cagaaaatat ctagcagtgt tatttgcttt ttttaacata tcctgcgcat tttactttgg
     8041 tatgctgaac tcacttggag gggatttcaa atatgagttt acagcagatg ttagcctttt
     8101 gctattatca actataatag ctttgttaac gttttttttt atacaggttg tcgttttttc
     8161 attttttgaa aaaatccgca tggataaaaa aatattatat aataataaca tctcgctaga
     8221 ttttattata ataattataa cgagttttgc catatttttc tcattgtatt ataaagtggg
     8281 cgtattaggc attaataaag atatcataga agatgcacca aagatggttt tttattttaa
     8341 ctctatattt caaccatctt atttgatttt ggtttacctt ttttacagat atgatagcgt
     8401 ccgtttgata tactatgcta attttatatt atacatatta cttttgctgg taagtggcca
     8461 aacaggccaa ttgcttattt tgttttgttt atatataatg cgtaaacgca ataattttaa
     8521 tttattgcgt ctttttgttc tctcattaat ggggataagt ttttatccta taataagaat
     8581 ttttaaagat gccattgtgc agtcagttaa tggtaatgaa tctttagtgg gagctttagt
     8641 tagtgtttat tctaatttgg attccgaact ctatttcaga tatttgttca ttacgctcga
     8701 aaggtttcag atagtaagta atatccatta catcattgag aatggtcggg tgctctattc
     8761 tggtttcctt cacactggat catctgatac atttttctct ctttattgga tattttctgc
     8821 aatttctaag ttgctaggtt atcatacagc tcatataatt tctgcacagg attttatggc
     8881 tattaatatt aatggtattc cgacttggtc ttctcaaata ggagtgttag gttactttta
     8941 tttttaccaa gtttatgcag tattaataat aatgtttatg ttctttatac tatttatatc
     9001 aataactctt agtaaatatt taagccgtaa taaagaaata ataaatttaa cttggttttt
     9061 atcattaatg ctcttgtgtc atggttggtt tattccgttc ataaattatg tccaatgctt
     9121 ggctgtattt gtagcgctga tctctattct aaagactaga actcaaaaat ttgttcggaa
     9181 ctaaagaggg caattagtga aaaggaaagt tgttgttttt agaagtaaat tattaccctt
     9241 ttcagaaact tttataaaag agcaggtgtt atcctattca aaatgggaac ctgtattaat
     9301 ggggcattgt ttactaaaaa acagagtaaa tttagatgat ataacaaaca aaattgtatt
     9361 tgactataat gatagtggag tctcttgttt taagaggaaa gtttataagt tttttaatgt
     9421 ctgccccata aaagataaaa aaataatagc gcaacaaaga gctgatttga tacatattca
     9481 ttttggtgtc gacgctgtag ttaactggtc aacctttaag caattcaaaa agcctattgt
     9541 tattacgctt catggatacg atattaatat aaaaaaagaa tggtgggctt ctggtaatga
     9601 gggtagacac ttaaaaagat atccagaacg attattagaa atttctaaat acaacaatgt
     9661 gtattttatt gcagtatcta aagctataaa agagagagcg atagcatttg gtataccacc
     9721 taaaaaaata agcgtatcat acattgggat cgatactttt agttttaaaa atagtatgac
     9781 tccttttaat gaacgtagac aaatattatt tgtcggtcgt ttagttgaaa aaaaaggatg
     9841 tgagattcta ataaatgcat atgcaaaact acatcgacaa cattatggtt atccacttaa
     9901 aataataggg gatggcccat taaaacatca gttggaaacc tacgcaaaaa agctaaattt
     9961 aaatgtacaa tttactggag tattatcaaa gcgagaaatt attgctgagt taaacaaaac
    10021 agcaattttt tgtttaccaa gtatacatgc ctcaagtggt gatgctgaag gatttggtct
    10081 ggttttactg gaggctgctg cgtgtggtgt tcctgtaata acctcagcat tcggaggggc
    10141 tgaagagggg gtgattcatg gtttgactgg attttcattc ccagaaaaag atactaacag
    10201 attatcatac tatcttaatc tacttctttc tgatccaaat ctaatggagt caatgggtaa
    10261 agccggtcaa cagttcgtgg ctgataactt tgatttgaaa aaatgtactg aagaattaga
    10321 ggttatctac aatagtataa tggaacaatt ttttgaaaaa taattggaat tggtatgtta
    10381 aataagagca agatagatgt tgtagttcca agttataatc gtctggatta cttattgcaa
    10441 tcaattaaaa gtattaaaaa acaatcgtac acggccaacc aaataatagt agtagatgat
    10501 gattctgagt tttctgagaa agacttttgg tcaggtttaa aaagggaaaa catttgtact
    10561 gatagaattc ttttttataa gaaagaaaaa aatctaggtg catgccattc aagaaatctt
    10621 ggagtacaac ttagtaagtc tgaatatgtc gcatttcttg atgatgacga tctctgggag
    10681 ataaatcatc ttgaaggact tataaattgt tttcagaatg atgatatagt attagcttat
    10741 tctgggaaat tgattaaaaa tttcactaca ggtaaagaac gaaaaagtct taatgtaatt
    10801 cctgatgaac aacagtttct ttcattatta aaatgcaatt acccgggttc aacatcttca
    10861 ataatggtaa ggaaaaaaag tttttttgct gctggagggt ttgatgagac tctccctgca
    10921 attcaagatt atgactttta tctccgcatt gtaaaagagg ggagaatcac gactagtaat
    10981 atttttactt taatctatag agttgatact cccgtaaaaa ttactaatca attatataaa
    11041 gcccgtaagg cttattattt aattattgaa aagttagtgc cccagcatcg aaaagtgctt
    11101 gctaaaacaa tatctattca aaatattaag aaagcagtaa taaataaaaa aatgaattat
    11161 attattctct ttagttttga ttatattaaa aatgcgttga ggaaaggctg aaaatgaaat
    11221 atgattacct tattgtcggt tcgggacttt atggttccgt gtttgcttat gaaatgaaaa
    11281 aaagaggatt tacggtaaaa atcatcgaga aaagagaaca tgtgggtggg aatgtctact
    11341 gtaaaaatat tgatggtata aatgtgcatg tttatggggc acatattttt catactaatg
    11401 ataaaaaaat atgggattat gtaaatgaat tagtagaatt taaccgtttc acaaattctc
    11461 ctcttgcctt ttcaaaagga aaattgttca acctgccctt taatatgaat acattctatc
    11521 aattgtgggg cgtaacaagt ccagatgagg caaaagcaat aattgaacat caaagtaaac
    11581 aaccaaatgt tattgaacca caaaatcttg aagagcaggc tatatctcta gtaggtaaag
    11641 atatatttaa tactttgatc aaagagtaca cagaaaagca atggggtaga aactgcaaag
    11701 agctcccacc ttttattatt aaacggttac cagtaagatt tacttttgat aataattact
    11761 ttaatgataa atatcaagga attccaattg gcggatataa caagttaata gaatccttac
    11821 ttgatggtgt ggaagttgaa accaatactg atttctttga aaacaaagaa tattggctca
    11881 aatctgcaaa taaaatagtt tatactggtc caatagatag gttttttggt tttgaatttg
    11941 gtgaattagc ttatagaaca cttgagttcg aacatgagga aataaataaa tccaattttc
    12001 aagggaatgc tgtaattaat tatattgatt ctcaataccc atacactaga attattgagc
    12061 ataaacactt tgagtttggc tgccaagaaa aaacaattat aactaaagaa tatcctaaag
    12121 aatattctaa aggagatgaa ccatactatc ctgtaaatga tagttataat aatagattgt
    12181 tttcttatta taaacaaaaa gcaaaaaact tggataatgt gatttttggt ggtcgacttg
    12241 ctgagtataa atattatgat atgcatcagg ttattgcctc agcattaacc gcggttgaaa
    12301 aggaattatg tacagaatga aaaaggttat tgctgtcgta gttacattca aacgaaagtc
    12361 tctattagaa gatgttatca aaggtcttgc tgaacagtct taccctttga gtgaaatcat
    12421 tatcattgat aatcatagtt gcgatggtac tgatattcta gttgacaact tgagaaaagt
    12481 atatgcaacg tctaaaataa gttattatga cactggtgat aatttagggg gcgcaggggg
    12541 ctttgcgtat ggatttgagg tggcgcaaaa aagtgatttt gaataccttt ggttaatgga
    12601 tgatgacctt ttgcctgagt ctgattgttt ggaaaatctt ttggctactg gatgtgatgg
    12661 aattatacag cctataagat ttaataagga tggttcatgc gcagaaattt cgccgataga
    12721 atatgatctt gactctgtat tcataattaa ccctaagaaa aaaaccgtta aagatattat
    12781 tgagttaaaa aatgtggata tacctgtacc tatcgcaggt gttccttttg aaggcccttt
    12841 aatctcaaaa tctgttgttg ataaaatagg taaacctgag tccaaatttt ttatttttaa
    12901 tgatgatttg gattattcta ttcgagcgcg aaatgccggt tttaaaattt tgtgtcaacc
    12961 aaaagctact gcgattagat tattggtaaa taatcaaagt aatgatctta agtcctggaa
    13021 aggctatttt atgttacgta accattttta tatattacgt acgcatggaa aaaatttttt
    13081 tgttaaatca aggccgatta tattgacatt aggatacgca gcattatcta tatttcgagg
    13141 tgatctagat gtggctctaa aagtatgtag ggcttataga gattcttact atttgagtaa
    13201 caatgacaga tttaagccat aataaatttc tgttaatatc aactcattaa tacccgttat
    13261 atatttgctt ctacgtggtg ttgatttttt agacttctaa tttattaaat attactcaat
    13321 agaatttatt tattttgacg tacgctgata tatcgggatt ttttgattgt tgccctaaat
    13381 taaattgatg gtatgatatg tgccgatatt tcatttatcg ctaaatctga tttttatttg
    13441 gatatttttt acatacaata atttatcgta aataatgttt agcctttagg ctatcgcccc
    13501 tgacaggagt aaacaatgtc aaagcaacag atcggcgtag tcggtatggc agtgatgggg
    13561 cgcaaccttg cgctcaacat cgaaagccgt ggttataccg tctctatttt caaccgttcc
    13621 cgtgaaaaga cggaagaagt gattgccgaa aatccaggca agaaactggt tccttactat
    13681 acggtgaaag agtttgttga atctctggaa acacctcgtc gcatcctgtt aatggtgaaa
    13741 gcaggtgcag gcacggatgc tgctattgat tccctcaaac catatctcga taaaggtgac
    13801 atcatcattg atggtggtaa caccttcttc caggatacca tccgtcgtaa ccgtgagctt
    13861 tctgccgaag gctttaactt cattggtacc ggtgtctccg gtggtgaaga aggcgcgctg
    13921 aaaggtcctt ccattatgcc tggtggccag aaagaagcct atgaactggt tgcaccgatc
    13981 ctgaccaaaa tcgccgcagt ggctgaagac ggtgagccat gcgttaccta tattggtgcc
    14041 gatggtgcag gtcactatgt gaagatggtt cacaacggta ttgaatacgg tgatatgcag
    14101 ctgattgctg aagcctattc tctgctaaaa ggtggcctga acctcaccaa cgaagaactg
    14161 gcgcagacct ttaccgagtg gaataacggt gaactgagca gctatctgat cgacatcacc
    14221 aaagatatct tcaccaaaaa agatgaagac ggtaactacc tggttgatgt gattctggat
    14281 gaggcggcta acaaaggtac cggtaaatgg accagccaga gcgcgctgga tctcggcgaa
    14341 ccgctgtcgc tgattaccga gtctgtgttt gcacgttata tctcttctct gaaagatcag
    14401 cgtgttgccg catctaaagt actgactggt ccgcaagcac agccagcagg cgacaaggct
    14461 gagttcatcg aaaaagttcg tcgtgcgctg tatctgggca aaatcgtttc ttacgcccaa
    14521 ggcttctctc agctgcgtgc tgcgtctgaa gagtacaact gggatctgaa ctacggcgaa
    14581 atcgcgaaga ttttccgtgc tggctgcatc atccgtgcgc agttcctgca gaaaatcacc
    14641 gatgcctatg ccgaaaatcc gcagatcgct aacctgctgc tggctccgta cttcaagcaa
    14701 attgccgatg actaccagca ggcgctgcgc gatgtcgtcg cttatgcggt acagaacggt
    14761 atcccggttc cgaccttcgc cgctgcggtt gcctattatg acagctaccg cgccgcagtt
    14821 ctgcctgcga acctgatcca ggcacagcgt gactatttcg gtgcgcatac ttataagcgc
    14881 attgataaag aaggtgtgtt ccataccgaa tggctggatt aatctgattt aaatcaatta
    14941 atcaatgcaa ggcccggaga aaccctccgg gcttttttat tatacaaagc ggcaggttat
    15001 ggcctttttt tataatttat ggttaaaaaa gcgatataat acagagccgc acagcaggat
    15061 cgctgccttg acagttcatc tacatcagcg ttaaaaatcc cgcagtagat gaagttgtgg
    15121 tggtggatta atgaccactc taaatgttta accggaagaa gtcagagcta atgaaaataa
    15181 caatttcagg aacaggttat gttggtcttt caaatggtat tctgattgcg caaaatcacg
    15241 aagtggttgc actggatatc gttcaggcca aagtggacat gcttaacaag aagcagtcac
    15301 cgattgttga taaggagatt gaagagtatc tggcgactaa agatctcaat ttccgcgcta
    15361 cgacagataa gtatgacgcg tataaaaatg ccgattacgt tattattgcc acacctaccg
    15421 attatgatcc gaaaacaaat tattttaata cctcaagcgt ggaagcggtc atccgtgatg
    15481 tgacagaaat taatcccaac gcggtaatgg tgattaaatc tacgattccg gtaggtttca
    15541 ccaaatcaat taaagaacgt ctgggtatcg ataaccttat tttctctccg gaatttctcc
    15601 gtgaaggtaa agctctttac gataatctcc atccttcacg tattgtcatt ggtgagcgtt
    15661 cagaacgcgc agaacgtttt gcggcgttat tacaggaagg cgcgattaag caaaatatcc
    15721 caaccctgtt taccgactcc actgaagcag aagcgattaa acttttcgct aatacctatc
    15781 tggcgatgcg cgtagcatac tttaatgaac tggatagcta tgcagaaagt ttaggtctga
    15841 atactcgcca gattatcgaa ggcgtttgtc tcgatccgcg tattggcaac cattacaaca
    15901 atccgtcgtt tggttatggt ggctattgcc tgccgaaaga taccaagcag ttgctggcga
    15961 actatcagtc tgtgccgaat aacctgatct cggcaattgt cgatgctaac cgcacgcgta
    16021 aagattttat tgccgatgcc attttgtcac gcaagccgca agtggtgggt atttatcgtc
    16081 tgattatgaa gagcggttca gataatttcc gcgcgtcttc cattcagggg attatgaaac
    16141 gtatcaaagc gaaaggcgta gaagtgatca tatacgagcc agtgatgaaa gaagactcat
    16201 tcttcaactc tcgcctggaa cgagatctcg ccaccttcaa acaacaagcc gacgtcatta
    16261 tctccaaccg tatggcagaa gagcttaagg atgtggccga taaggtatac acccgcgatc
    16321 tctttggcag cgactaacat cctgttatca gggcgatttt cgccctgatt ctcttatgtt
    16381 ccctttataa taattcatta tttttatcat ttatcctata gcattcatgg cgattatcgc
    16441 taaactatgg cgacttaaat ttttcgtccg ttagggtaat gatgagagta gaaaataata
    16501 atgtttctgg gcaaaaccat gacccggaac agattgattt gattgattta ctagcccaac
    16561 tatggcgcgg caagatgact atcatcattt ctgtcattgt ggctatagtt ctggctatcg
    16621 ggtacctgta cgtagccaaa gaaaaatgga cttccacagc tatcgttact caacctgatg
    16681 tggggcaaat cgctggttat aacaatgcca taaatgtgat ttatggttcg gcagcgccga
    16741 aagtgtctga gatccagtcg atgttaattg gtcgtttcag cacgacattt tctgcattag
    16801 cagaaacgct ggataatcag gaagaagcag agaagctgac cattgagcct accgttaaaa
    16861 accagtcgtt accattggcg gtgtcttatg ttggtgaaac acctgaaggt gcgcagaaac
    16921 agttggcgaa gtatatccag caagttgacg accaggttaa tgaagaactg gaaaaagacc
    16981 tgaaagacaa catcgcgctg cgtatgaaaa acttgcagga ttctctgaaa actcaggaag
    17041 tagtcgccca ggagcagaaa gatctgcgta tccgtcagat tcaggaagcg ttgcagtatg
    17101 cgaatcaggc gcaggtgaca aagccgcaga ttcagcagac acaggacgta acccaagata
    17161 ctatgttcct tttggggagc gaagcgctgg agtcgatgat taagcacgag gcgactcgtc
    17221 cgttggtgtt ctcatcaaac tactatcaga ctcgtcaaaa cctgctggat atcgacaatc
    17281 ttgacgttga taaacttgat attcatgctt accgctatgt aatgaaaccg acgttaccta
    17341 ttcgtcgcga tagtccgaaa aaggcaatta ccttgattct ggcggtgctg ctgggcggca
    17401 tggttggcgc gggagttgta ctggggcgta atttgctata tggatataaa taatatctta
    17461 tacgcaaaaa agtatcacta atatatctgt agaaaatcac atcacctttt cagaatatga
    17521 taaggtgatg cttgttaaat ttatttttat tttctgaata atgctttcaa tttttactac
    17581 tccaataatc tgacggatat ttcatagtgt aaaaatcagt tgtcaatctt ctctgtataa
    17641 aatatagatg ttgtttaatg tctatttata tcgataatat tttattttat taaacagtat
    17701 tgttcacact ctacgcgaat tatcgactat ttctcatatg tgcttgttgt tttgcaattc
    17761 ttcttccatt tttagtatgt tttttcttct gctttttatt ttctttgcgg gagagccaaa
    17821 cattattgac cactcttcag tactttttgt tattaatgag ttagcaccaa ctgcagtgcc
    17881 ttcttttaag gttatattag gcataattat tgattgggaa ccaactatga catgtttttc
    17941 aagagtcact tctccacgta ttacacctgt gtattgggat ggaacacagg ggtttgtcat
    18001 gaattttcct gagtaatcat cactttttga gtatattttt actccctgag aaataccgct
    18061 aaaatccatc attgtaacac cacttcctgc aaaaatcgca gaatatccac cgatgtgaat
    18121 atatgaacct aaatttaaat atcctccatt atttgcgatg attgttgtaa aagcatcaat
    18181 gcgtacatta cttccaattg aaatattttc aattccgact atattacaat tttttgcaat
    18241 aagtacgttt tctccaatat gtttgaaacc acattttatt aggtgttcgg tatcgtaata
    18301 tccaatgtca aatgttttga gcgacatatt ttcacctttt atatgagtta gtacttaaat
    18361 tctaatctaa aatctgatcg tcgtcagcat ttattatgaa ttttctgata tcaggttgct
    18421 cttaacagtt ctattgttgt cttttacttg gctgttatta atattataca taaatgttcc
    18481 tatgttaggg ttgtattgtt ttaactatgt tatctttctt gagacacttt ttgaagtatt
    18541 ctcggatgcg acaacaacat cgcatccgct taccccgcaa ctcactgatg ccgtttgcgc
    18601 aggttctcaa tcaccgtcgt taaatccaga tcctgatcct gcaacagcac cagcaggtga
    18661 tacatcaagt cagatgcctc gttggtcagc tcaaagcggt catgtaccgt tgctgccagt
    18721 gcggtttcca cgccttcttc gcccactttc tgcgcaatgc gtttggtgcc gctggcatac
    18781 agtttggcgg tgtaggaggt ttccgggtcg gcagatttac gttcggcgag cagttgttcc
    18841 agttgataca ggaacagcca ctggtgagcg gtgtcgccga agcagctgct ggtgcctttg
    18901 tggcaggttg gcccgattgg atttgccagc accagtaacg tgtcgttgtc gcagtccgga
    18961 gcaatactca ccacattgag aaaattgccc gacgtttcgc ctttggtcca cagtcgctgt
    19021 ttagtgcgcg agaagaaggt gacattgccg ctttctatgg ttttgtccag agcttccggg
    19081 ttcatatagc ccagcattaa cacttcaccg gataccgcgt gctgcacaat caccggcata
    19141 agtccgtcgg ttttttccca gtccagttcg cgacgttgtt gttctgttaa catatcctga
    19201 tctccacgcc ctgtgttgca aggtacgctt ttaattcacc aatattgatt atttgtttgt
    19261 ggaatacaga agctgccagc gcaccgtcaa cgtcggcatc gcggaaggct tcgaggaagt
    19321 gttccatggt gcccgcgcca ccggaagcaa tcagcgggac gtggcaaact tcacgcactt
    19381 ttttcagctg ctctaggtcg taaccgttac gcacgccgtc ctgattcatc atgttaagga
    19441 cgatttctcc ggcaccgcgt ttttgcactt cctgtaccca gtcgagcgtt tcccactgag
    19501 t
//
