LOCUS       LC550085               19236 bp    DNA     linear   BCT 04-AUG-2020
DEFINITION  Escherichia coli OT-569 genes for O-antigen biosynthesis gene
            cluster, complete cds.
ACCESSION   LC550085
VERSION     LC550085.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S. and Lee,K.
  TITLE     Additional O-genotyping PCR primers targeting novel E. coli and
            Shigella-unique O-genotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19236)
  AUTHORS   Iguchi,A., Iyoda,S. and Lee,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2020) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuenkibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..19236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="OT-569"
                     /db_xref="taxon:562"
                     /note="OgN33 O-antigen biosynthesis gene cluster"
     gene            253..1647
                     /gene="wcaM"
     CDS             253..1647
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BCG06361.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFGEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQLIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            1822..2715
                     /gene="galF"
     CDS             1822..2715
                     /gene="galF"
                     /note="OgN33_1"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BCG06362.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            3280..4173
                     /gene="rmlB"
     CDS             3280..4173
                     /gene="rmlB"
                     /note="OgN33_2"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BCG06363.1"
                     /translation="MARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLL
                     EAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTAYAPSSP
                     YSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIY
                     GKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVP
                     KEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWYLSNTKW
                     VDNVKSGAYQSWIEQNYEGRQ"
     gene            4173..5072
                     /gene="rmlD"
     CDS             4173..5072
                     /gene="rmlD"
                     /note="OgN33_3"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="BCG06364.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKTDVAGLYHLVAS
                     GTTTWYDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            5130..6008
                     /gene="rmlA"
     CDS             5130..6008
                     /gene="rmlA"
                     /note="OgN33_4"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BCG06365.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLASIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKRFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            6013..6558
                     /gene="rmlC"
     CDS             6013..6558
                     /gene="rmlC"
                     /note="OgN33_5"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="BCG06366.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSADNKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPESDRGIIWDDPELNIDWPLT
                     DLGNDVLLLSSKDKTHPTLNN"
     gene            6655..7770
                     /gene="wzy"
     CDS             6655..7770
                     /gene="wzy"
                     /note="OgN33_6"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BCG06367.1"
                     /translation="MLFISLNVLLFISSFTNNRTIFKITFFMTACYCSMSFGRGFDWI
                     NYYDVYQNVELYDAPFEPGYLNVLRTFNWLGFSYPMQNFVVVLFLFYCVYVFVSKTQN
                     PSLAFFTIFCFMGHYILSEQIRQAIAICIILLFFDFFIKRKVLKGTVVIALAMCFHIS
                     AVFCFIYFFIVNTNAKYSNVKFSIYCSVFLLVSYYMWSNPAIISTVPILYNKFVSYTE
                     AYTEGFISIERIISSKVVMIYIFLLCLSIWLLKKYNLKSLNGSIKALVFMTLSKLTIF
                     FGRFQYYMVPILILGFDEYFAKYKIKNKVNVHRLIYASCLFIISLVPLWTPSTFESIN
                     DSLYFNASPKEIESGIMRRCKTLNHYDPQNGAIWRCK"
     CDS             7810..8751
                     /note="OgN33_7"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /product="glycosyltransferase"
                     /protein_id="BCG06368.1"
                     /translation="MAIKEIEITAIVPTIGRETVFDSLKSIISQTYKVFEIIIVYDGA
                     DFVIFEGKINKYFEGIKNREPTKYKVINVGPFSGGNVARQKAIETARTEYVALLDDDD
                     IWLDNHIEDYISLLEKRKNHLPVLLSCAANIILTESKVVKVPTRFISEGETIPEYLFK
                     IRNIIADCGFIQSSLLMFSRQLAIDVPFNTELKFHQDIDWLLRLSQSNIHFDFIQSRN
                     NTVTYNSTPLSVSKKITPSESKYWAIMTFRNRRCMGDFILTQSYNYARNNGSLLDEFV
                     VLISSIRYARPGVFSLIRYFIKLSRIDVLIKKFLKDK"
     CDS             8755..9837
                     /note="OgN33_8"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="glycosyltransferase"
                     /protein_id="BCG06369.1"
                     /translation="MVKKWVTIFERYEDFHFDKDVGQLAYHAKVTLNYNVELWCKKGE
                     HTLKKQLFKIKELECGAKNTKVSFRVIWHITKNARKIDYLNIFHFRIYSLVYAFFYKV
                     INPRGKVILKCDGSERCYPYNDNKLKNRLLFYFLKKIKLINIFLAEHLSLVEYYKSKG
                     LPALFCPNGTSPAYYSCKLPVKSNIVPTIIFVGTCGDDRKNAKLAVEAVSKLKFDCRI
                     FFLGGETELFNKWFKKWSIGKPHDVIQRYIFCGYVNDVSRIIHYYDEAHVFLMTSVHE
                     GYPLSLAQAAWRGCYPILSEGSGGGDMNIAGCAAIFHNEESLIDILDKSLSNLSATIA
                     KGKKSRLYALNNNDWRLIIRKIEELI"
     gene            9839..11119
                     /gene="wzx"
     CDS             9839..11119
                     /gene="wzx"
                     /note="OgN33_9"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BCG06370.1"
                     /translation="MTKIIFKTAALYMFFSVINQAIGLFIQLILMQGVSINNYGIYAI
                     NFEAMALMQLVLANAYRNFYLQKIRNNISEFEINNLITYQIVNGTISILIFGTLISLA
                     YKLSLAVSAFLIISNVLSSLLLPLQANWLANNERFKIIIKDFTISIVSLIAVIISVKM
                     MKFSVNQITITQFIVYGGISFVILFIFKDKLKFKAVFSKNIFSCFHFDKTLYVFILIF
                     TINALHNKYGGLFLRHYSNEMQTALYLAAFKFINPLFFIQTSLISAFMPNFIKNKNFE
                     FDIKVYFTFAIPGILIALLLYFFFPFILGVLRIEKYLPAYEIIKVASLFVFIVFIYGA
                     MSNYISVSGGQSFILKVNILALTLMCIASFLLINDNNISFMLINVFVFAEALICILYY
                     AYLSKIKVKTSVLFLISPLLCMLIIIVSFLQHYA"
     CDS             11128..12018
                     /note="OgN33_10"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /product="glycosyltransferase"
                     /protein_id="BCG06371.1"
                     /translation="MIRLTIIIVIYGKECFDSRTLQSLKLCPLHQNITLFIFNNGPRL
                     LSEDKCYELFDEQIKKNKINLILKQNINNIPLSVIYNDAIKENAASDYFMILDDDTEI
                     PSSYFAFNANRFDFDLSVPLIKTGESEEFFYPIVDGIIPIKKQSVDDYEEIYTISSGL
                     MISKSLTETFKCYFNDVFDTRFAFYGIDFNFFRRLRIIKHHGHYFRLFIAEPIRHSLS
                     RFDGEQTEWRNIERIYDVVLSLKYYEKRNMVKVVKLARLVSSSLLKFKFRSIKAIFYS
                     LLVGMHPRSKKYISNKKIIK"
     CDS             12080..12973
                     /note="OgN33_11"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf12"
                     /product="glycosyltranslocase"
                     /protein_id="BCG06372.1"
                     /translation="MNHVTAILVTYNPDYELLPHVVEQITQQVDSVILIDNASTCSEK
                     IFSVIKEYSNIHFYSLSENIGLAAAQNLAISKVQEKCNYVIFFDQDSVIQKDLIKQLL
                     IADFRLRQAGENVAAVGPSFIDPTNNMIYPATVYKGPFIKKVDVTSQPIEATFIIASG
                     CLIKTSAINEIGLMYDGFFIDYIDVEWCLRAKSKGYSVYVIPNAKMYHTIGDSRITIL
                     GRTISIHNPMRRYYLIRNSILICKLKYIPIGYKIRECMFNIIRAILSLLLNNNKKLTL
                     KMIFRGIIDGVKGITGRVEIK"
     CDS             12970..14064
                     /note="OgN33_12"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf13"
                     /product="glycosyltranslocase"
                     /protein_id="BCG06373.1"
                     /translation="MRKRLAHIQVVPKMSGVQQISFDILKNLDPDEFDLYLICGDGTE
                     IENFKKRFQSINVKVIVIPELKREICFSDIKAFFRLLALFKRHKIDIVHTNSTKPGVI
                     ARIAAKFAGVRKIVHTVHGIAFHAEMNTFKRLFYWLVEYFSCFFGDVNVLVNKHYLMY
                     YPFINNMVVYNGVDFGQFTGSKSVSDQLHFAFMARLDDQKNPFEFIEAIKIIHDTHPH
                     LLSNVKFTLAGDGELAEQCRFLIVRYGLENKVNMPGWIIDKNKFFSDVDVICQPSKWE
                     AFGLVFAEAAYFKIPSIARAVEGVPEVIINGKTGLLYNKSEDELSVLMIKLINNPSLI
                     SELGEQAYSYVTNKFSLNTMLANYKQLYTS"
     gene            14202..15608
                     /gene="gnd"
     CDS             14202..15608
                     /gene="gnd"
                     /note="OgN33_13"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BCG06374.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILNKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            15830..17023
                     /gene="ugd"
     CDS             15830..17023
                     /gene="ugd"
                     /note="OgN33_14"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BCG06375.1"
                     /translation="MFNRKKSELMKITISGTGYVGLSNGILIAQNHEVVALDIVQAKV
                     DMLNKKQSPIVDKEIEEYLATKELNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFN
                     TSSVEAVIRDVTKINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYD
                     NLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVA
                     YFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQS
                     VPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRI
                     KAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRD
                     LFGSD"
     gene            17113..18147
                     /gene="wzz"
     CDS             17113..18147
                     /gene="wzz"
                     /note="OgN33_15"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BCG06376.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQPLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIENLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(18243..18854)
                     /gene="hisI"
     CDS             complement(18243..18854)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BCG06377.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     5665 a   3600 c   4170 g   5801 t
ORIGIN
        1 tcggcattcc ggtggtttct actctgcata gcggaatacc ggaactggtt gaggccgata
       61 aatccggctg gctggtgcct gagaacgatg ctcgcgcact ggcgcaacga ctggcggcgt
      121 ttagccaact ggacaccgac gaactggctc cggtcgtcaa acgtgcgcgc gaaaaagtcg
      181 aacacgattt taaccagcag gtgattaacc gagaactcgc cagcttgttg caggctttat
      241 agaggagaac gtatgccatt taaaaaactc tcccgacgca ccttcctgac ggcaagctcc
      301 gcgcttgcct tcctccatac ccctttcgct cgcgcacttc ccgcccgaca aagcgttaac
      361 attaacgact acaaccctca cgactggatc gcctcattta aacaagcctt cggcgaaggg
      421 caaacggtcg tcgtgcctgc cggattggtt tgcgacaata tcaacaccgg catcttcatt
      481 cctgcaggta aaacgttaca catccttgga agcctgcgcg gcaacggcag agggcgattt
      541 gtcttacagg acggcagcca ggtgacaggg ggggagggcg gcagtatgca taacatcacc
      601 ctggatgtgc gcggctctga ctgcaccatc aaagggctga ctatgagcgg ctttggtccg
      661 gtaacgcaga tttatatcgg cggcaaaaac aaacgggtca tgcgcaacct gaccatcgat
      721 aacctgaccg ttagccatgc taattacgcc atcttacgcc agggatttca taaccagctt
      781 atcggtgcca acatcaccaa ctgtaagttc agcgacttac aaggcgacgc cattgaatgg
      841 aacgtggcga ttaacgaccg tgatattttg atatctgacc atgtcatcga gcgcatcaac
      901 tgtaccaacg gcaaaatcaa ctggggtatc ggcataggcc ttgcgggaag tacctacgat
      961 aataactacc cggaagacca ggcagtgaaa aactttgtcg tggcgaatat cacgggatcg
     1021 gattgtcggc agttgatcca tgttgaaaat ggtaaacatt ttgttattcg taatatcaaa
     1081 gcccgcaata tcacgccgga tttcagtaag aaagcaggta ttgataacgc cacagtcgct
     1141 atttacggtt gtgacaattt cgtgattgat aatattgaaa tgattaatag cgccgggatg
     1201 ttaatcggct atggggtaat taaaggcaaa tatctctcga taccgcaaaa tttccgagtg
     1261 aataatattc aactggataa tacccacctt gcttacaaat tgcgcggtat ccaaatctcc
     1321 gccgggaatg ctgtctcctt tgtggcgctg actaacattg agatgaagcg tgcctcgctg
     1381 gagcttcaca ataaaccgca acatcttttt atgcgtaata tcaaagtgat gcaggaatcc
     1441 tctgttggac ccgcattgag catgaacttt gacatgcgca aagacgttcg cggcgtcttt
     1501 atggcgaaaa aagaaaccct gctatctctt gcaaatgttc atgcggtgaa tgagaaagga
     1561 caaagctccg tcgatatcga tagggttaat caccatattg ttaatgtgga aaagattaac
     1621 tttagattgc cggaacggag ggagtagatt tgcgaccatt cctggaaaaa tggagccata
     1681 cttaggaaca atgctactgc aatccacaac gaagcggcgt aacatcacaa gtaattcagt
     1741 aatcaattca aggtaattga tgctggcgaa aaaaatcgaa caagctataa ttcagcaacc
     1801 attttacagg tggatgaaat aatgacgaat ttaaaagcag ttattcctgt agcgggtctt
     1861 gggatgcata tgttgcctgc cactaaggcg attcccaaag agatgctacc gatcgtcgac
     1921 aagccaatga ttcagtacat tgttgacgag attgtggctg cagggatcaa agaaatcctc
     1981 ctggtaactc acgcgtccaa gaacgcggtc gaaaaccact tcgacacctc ttatgaatta
     2041 gaatctctcc ttgaacagcg cgtgaagcgt cagctgctgg cggaagtgca gtccatctgt
     2101 ccgccgggtg tgaccattat gaacgtgcgt cagggcgaac ctttaggttt gggccactcc
     2161 attttatgtg cacgacccgc cattggtgac aacccatttg tcgtggtgct gccagacgtt
     2221 gtgatcgacg acgccagcgc cgacccgctg cgctacaacc ttgctgccat gattgcgcgc
     2281 ttcaacgaaa caggacgcag ccaggtgctg gcaaaacgta tgccgggtga cctctctgaa
     2341 tactccgtca ttcagaccaa agaaccgctg gatcgcgaag gcaaagtcag ccgcatcgtg
     2401 gaatttatcg aaaaaccgga tcagccgcag acgctggact cagacatcat ggccgtgggt
     2461 cgctatgtgc tttctgccga tatttggccg gaacttgaac gcactcagcc tggtgcatgg
     2521 gggcgtattc agctgactga tgccatcgct gaactggcga aaaaacagtc cgttgatgcc
     2581 atgctgatga caggtgacag ctacgactgc ggtaaaaaaa tgggttatat gcaggcattt
     2641 gtgaagtatg gactgcgcaa cctgaaagaa ggggcgaagt tccgtaaagg gattgagaag
     2701 ctgttaagcg aataatgaaa atctgaccgg atgtaacggt tgataagaaa attataacgg
     2761 cagtgaagat tcgtggcgaa agtaatttgt tgcgaatatt cctgccgttg ttttatataa
     2821 acaatcagaa taacaacgag ttagcaatag gattttagtc aaagttttcc aggattttcc
     2881 ttgtttccag agcggattgg taagacaatt agcgtttgaa tttttcgggt ttagcgcgag
     2941 tgggtaacgc tcgctacatc gtaggcatgc atgcagtgct ctggtagctg taaagccagg
     3001 ggcggtagcg tgcattaata cctctattaa tcaaacaaag agccgcttat ttcacagtat
     3061 gctctgaagt aatatggaat aaattaagtg aaaatacttg ttactggtgg cgcaggattt
     3121 attggttctg ctgtagttcg tcacattata aataatacgc aggatagtgt tgttaatgtc
     3181 gataaattaa cgtacgccgg aaacctggaa tcacttgctg atgtttctga ttccgaacgc
     3241 tatgtttttg aacatgcgga tatttgcgat gcagctgtaa tggcacggat ttttgctcag
     3301 catcagccgg atgcagtgat gcacctggca gctgaaagcc atgtggatcg ttcaattaca
     3361 ggccctgcgg catttattga aaccaatatt gttggtactt atgtcctttt agaggccgct
     3421 cggaattact ggtctgctct tgatggcgac aagaaaaata gcttccgttt tcatcatatt
     3481 tctactgacg aagtctatgg tgatttgcct catccagatg aagtaaataa taacgaagaa
     3541 ttacccttat ttactgagac gacagcttac gcgccaagca gcccttattc cgcatccaaa
     3601 gcatccagcg atcatttagt ccgtgcgtgg aaacgtacct atggtttacc gaccattgtg
     3661 actaactgtt cgaataacta cggtccttat cactttccgg aaaaattgat tccactagta
     3721 attcttaatg ctctggaagg taaggcattg cctatttatg gtaaagggga tcaaattcgc
     3781 gactggctgt atgttgaaga tcatgcacgt gcgttatata ccgtcgtaac cgaaggtaaa
     3841 gcgggtgaaa cttataacat tggtggacac aacgaaaaga aaaacatcga tgtagtgctc
     3901 actatttgtg atttgttgga tgagattgta ccgaaagaga aatcttatcg tgagcaaatt
     3961 acttatgttg ccgatcgccc gggacacgat cgccgttatg cgattgatgc agagaagatt
     4021 agccgcgaat tgggctggaa accgcaggaa acgtttgaga gcgggattcg taaaacggtg
     4081 gaatggtacc tgtccaatac aaaatgggtt gataatgtga aaagtggtgc ctatcaatcg
     4141 tggattgaac agaactatga gggccgccag taatgaatat tctccttttc ggcaaaacag
     4201 ggcaggtagg ttgggaacta cagcgtgctc tggcaccttt gggtaatttg attgctcttg
     4261 atgttcactc cactgattat tgtggtgatt ttagtaatcc tgaaggtgtg gctgaaaccg
     4321 tcaaaaaaat tcgccctgat gttattgtta atgctgcggc tcataccgca gtagataagg
     4381 ctgagtcaga acccgaattt gcacaattac tcaatgcgac cagcgttgaa tcaattgcaa
     4441 aagcggctaa tgaagttggg gcctgggtaa ttcattactc aactgactac gtatttccgg
     4501 gaaccggtga aataccatgg ctggagacgg atgcaaccgc accgctaaat gtttacggtg
     4561 aaaccaagtt agccggagaa aaagcgttac aggaacattg cgcgaagcat cttattttcc
     4621 gtaccagctg ggtatacgca ggtaaaggaa ataacttcgc caaaacgatg ttgcgtctgg
     4681 caaaagagcg tgaagaatta gcggttatta acgatcagtt tggtgcacca acgggtgctg
     4741 agctgctggc tgattgtacg gcacatgcta ttcgtgttgc actgaaaaaa acggatgtcg
     4801 caggcttgta ccatttggta gccagtggta ccacaacctg gtacgattat gctgcgctgg
     4861 tttttgaaga ggcgcgcaaa gcaggcattc cccttgcact caacaagctc aacgcagtac
     4921 caacaactgc ctatcctaca ccagctcgtc gtccacataa ctctcgcctt aatacagaaa
     4981 aatttcagca gaactttgcg cttgtcttgc ctgactggca ggttggcgtg aaacgcatgc
     5041 tcaacgaatt atttacgact acagcaattt aatagttttt gcatcttgtt cgtgatgatg
     5101 gagcaagatg aattaaaagg aatgatgaaa tgaaaacgcg taaaggtatt attttagcgg
     5161 gcggttctgg tactcgtctt tatcctgtga ctatggctgt cagtaaacag ctgttaccga
     5221 tttatgataa acctatgatc tattacccgt tgtctacact gatgttagcg agtattcgcg
     5281 atattctgat tatcagtacg ccacaggata ctcctcgttt tcaacaactg ctgggtgacg
     5341 ggagccagtg ggggctaaat cttcagtaca aagtgcaacc gagtccagat ggtcttgcgc
     5401 aggcatttat catcggtgaa gagtttatcg gtggtgatga ttgtgctttg gttctaggtg
     5461 ataatatctt ttacggtcac gatctgccga agttaatgga tgtcgctgtt aacaaagaaa
     5521 gtggtgcaac ggtatttgcc tatcacgtta atgatcctga acgctacggt gtcgttgagt
     5581 ttgataaaaa cggtacggca ataagcctgg aagaaaaacc gttacaacca aaaagtaatt
     5641 atgcggtaac cgggctttat ttctatgata acgacgttgt cgaaatggcg aaaaatctta
     5701 agccttctgc ccgcggtgaa ctggaaatta ccgatattaa ccgtatttat atggaacagg
     5761 ggcgtttatc cgttgccatg atggggcgtg gatatgcatg gctggacacg ggaacacatc
     5821 aaagtcttat tgaagcaagc aactttattg caacaattga agaacgtcag ggactgaaag
     5881 tttcctgtcc ggaagaaatt gcttaccgta aacggtttat tgatgctgag caggtgaaag
     5941 tattagccga accgctgaag aaaaatgctt atggtcagta tctgctcaaa atgattaaag
     6001 gttattaata aaatgaacgt aattaaaact gaaattccag acgtactgat ttttgaaccg
     6061 aaagtttttg gtgatgagcg tggtttcttt tttgagagct ttaaccagaa ggtttttgag
     6121 gaagctgtag gccgcaaagt tgaatttgtc caagataacc attcgaagtc ttgtaaaggt
     6181 gttttacgcg ggctgcatta tcagttagaa ccttatgcac aagggaaatt ggtgcgctgc
     6241 gttgttggtg aagtttttga cgtagctgtt gatattcgta aatcgtcacc aacttttggc
     6301 aaatgggttg gggtgaattt atctgctgac aataagcgac aattgtggat tcctgaggga
     6361 tttgcgcatg ggtttttggt gttgagtgag acggcggagt ttttgtataa gacgacgaat
     6421 tattatcatc cagagagtga tagaggaatt atctgggatg atccggagtt aaatatagat
     6481 tggcctctaa ctgatctagg caatgatgta ttgctactat cctcaaagga taaaacccat
     6541 ccaaccttga ataattagtt atctgaaaac ggaaacatct taatgtaata aggggcagca
     6601 tgctcaacca aaaaataata ttgttgcaga taagcgtgtt atcgggagag tgagatgctc
     6661 tttataagtt tgaatgtgtt gctttttatt agttccttta cgaataatag aacaatattt
     6721 aaaatcacct tttttatgac tgcatgctat tgcagtatgt ctttcggtag aggttttgat
     6781 tggataaatt attatgatgt gtatcagaat gttgagttgt atgatgcacc ttttgagcct
     6841 gggtatttaa atgtattgcg cacatttaat tggttgggct tttcttatcc aatgcaaaac
     6901 tttgttgtag tgctttttct attttattgt gtatatgttt ttgtatctaa aacacaaaac
     6961 ccgtcacttg cattttttac gattttttgt tttatggggc attatatact atctgagcaa
     7021 attaggcagg ctattgcaat atgtataata ttattattct ttgacttttt cataaagaga
     7081 aaagtactaa aaggaacagt ggttattgca ttagctatgt gttttcatat ttcagctgtt
     7141 ttttgtttta tatatttttt tatagtaaac acaaatgcta aatattctaa tgttaaattt
     7201 tcaatctact gttccgtttt tttgttagtt tcatattata tgtggagtaa tcctgcaatt
     7261 atatcaacag ttcccatatt atataataaa tttgtaagtt atacggaagc ttatactgaa
     7321 ggttttatta gtattgaaag gattattagt tctaaagttg tcatgattta tattttttta
     7381 ctatgcttat caatttggct actaaagaag tataatttaa aaagtttaaa tggtagtata
     7441 aaagctcttg tttttatgac actttcaaag cttacgatat tttttggaag gtttcaatat
     7501 tacatggtac cgatattaat attagggttt gatgaatact ttgctaaata taaaataaaa
     7561 aataaagtga atgtacatag gttaatatat gcttcttgtc tttttataat atccttagtt
     7621 ccgttatgga caccatcgac atttgagtca ataaacgatt ctctgtactt caacgcgtca
     7681 cctaaagaaa ttgaaagcgg tattatgcgt agatgtaaaa cactcaacca ctacgaccct
     7741 caaaatgggg cgatttggag atgcaaataa tatttcatct gcagtctatg attaaagagt
     7801 ttaaggcaaa tggcaataaa agaaattgag ataacagcta ttgtaccaac gatcggtcgt
     7861 gaaacggtat ttgattcact aaagtcaatt atatcgcaaa cttataaggt atttgaaatc
     7921 ataattgtgt atgatggtgc tgatttcgtt atttttgagg gaaaaatcaa caagtatttt
     7981 gagggtataa aaaatagaga accaactaaa tataaagtta ttaatgtagg accattttca
     8041 ggggggaatg tcgcaaggca gaaagctatt gaaacggcaa gaacagaata tgttgcttta
     8101 cttgatgacg atgatatatg gcttgacaat catatagagg attatatttc ccttttagag
     8161 aaaaggaaaa accatttacc agttcttctt tcatgtgccg cgaacattat acttactgag
     8221 tcgaaagttg taaaagtgcc aactcgtttc atttccgaag gtgaaacaat tccagaatat
     8281 ttgtttaaaa tacgaaatat tattgcggat tgtggattca tacagtcatc tttattaatg
     8341 tttagtaggc agttagcaat agatgtacca tttaataccg aattaaaatt ccatcaagat
     8401 attgattggc ttctcaggtt atcacagagt aacattcatt ttgactttat acagtccaga
     8461 aataatacag ttacctataa ctccacgcct ttaagtgttt ctaaaaaaat cactcccagt
     8521 gagtcaaagt attgggctat tatgacattt aggaatagaa gatgcatggg agattttatt
     8581 cttacccaat catataacta tgctcgaaat aatggttcat tacttgatga atttgtcgtg
     8641 ctaatctcat caattagata tgcaaggcct ggtgtttttt cgctaataag atacttcata
     8701 aaactaagca ggattgatgt cttaattaaa aaatttctaa aggataaata gtttatggtg
     8761 aaaaagtggg taactatctt tgaaagatat gaagattttc acttcgataa ggatgtgggg
     8821 caacttgcat accatgcaaa agtgacttta aactataacg tagaactttg gtgtaaaaag
     8881 ggcgagcata ccctaaaaaa acagttgttc aaaattaaag aattagaatg tggtgctaaa
     8941 aatactaaag tttcattcag agttatttgg catattacaa aaaacgcgcg aaaaattgat
     9001 tacctaaaca tctttcactt tagaatatat agtcttgtat atgctttttt ttataaagtg
     9061 attaaccctc gaggaaaagt tattttaaag tgcgatggtt ctgaacggtg ttatccttat
     9121 aatgacaata aactaaaaaa taggcttctt ttttattttt taaaaaaaat taaattaata
     9181 aatatatttc tagcagaaca tttatcgttg gttgagtatt ataagtctaa aggattgcca
     9241 gcgctgtttt gccctaatgg tacttcaccg gcgtattata gttgcaagtt gccagtaaaa
     9301 tcaaatatag tacctaccat aatatttgtg ggtacttgcg gtgatgatag gaaaaatgca
     9361 aagttagctg ttgaagctgt atcgaaactc aagttcgact gtagaatatt ttttttaggc
     9421 ggtgaaaccg aacttttcaa taaatggttt aaaaaatgga gtatcggaaa accccatgat
     9481 gttatacaac gttacatctt ctgtggatat gttaatgatg tttcacgcat aattcattat
     9541 tatgatgaag cacatgtttt tttaatgaca tctgttcatg aaggatatcc actttctctc
     9601 gcgcaggcag cctggagagg atgctatcct attctttctg aaggatcagg cgggggggat
     9661 atgaatattg caggatgtgc agctatcttt cataatgaag aaagtttaat tgatattctt
     9721 gacaaaagcc tttccaattt atcagctacg attgctaaag gtaaaaaaag taggttatat
     9781 gcattaaata ataacgactg gcgtttaatc ataagaaaaa ttgaagagct gatttaaaat
     9841 gactaaaatt atttttaaaa cagcagcgct ctatatgttt ttttcagtca taaatcaagc
     9901 tataggcctt tttatacagc taatcttgat gcaaggagtc tcaattaata actatggtat
     9961 ttatgcaata aattttgaag caatggctct catgcaatta gtattggcta atgcgtaccg
    10021 taatttttat ttgcaaaaaa tacgcaataa tatatcagag tttgaaataa ataatcttat
    10081 cacttatcaa atagttaatg ggacaatttc gattttaata tttggtactt taatttcatt
    10141 ggcatataaa ttaagtttgg ctgtatctgc ttttttaatt atatctaatg ttttatcatc
    10201 tttacttctt ccattacaag caaattggtt ggcgaataat gagagattca agattattat
    10261 aaaggatttt accatatcaa ttgtgtcgct aattgctgtg attatatcag tgaaaatgat
    10321 gaaattttca gtgaatcaaa tcacaataac acaatttatc gtctatggtg gtatttcatt
    10381 tgtcatttta tttattttta aagataaatt aaaattcaag gccgttttca gcaagaatat
    10441 tttttcatgt tttcattttg ataagacgtt gtatgtattt atacttattt tcacgataaa
    10501 tgcacttcat aataaatacg gaggattgtt cttaagacac tattctaatg aaatgcaaac
    10561 agcattatat ttggcggcat ttaaatttat aaacccgctt ttttttatac aaacatcgtt
    10621 aatctccgct tttatgccaa attttattaa aaataaaaac tttgagtttg acattaaggt
    10681 ttattttact tttgctatcc ccggtatttt aattgcttta ctgctttatt ttttctttcc
    10741 gtttatactg ggggttctca gaatcgaaaa atacttgcct gcttatgaaa ttatcaaggt
    10801 tgcttccttg tttgtgttta ttgtttttat ttatggcgca atgtcgaatt atatatccgt
    10861 aagcggtggg cagtctttca tactaaaggt aaatattctc gcactaacac tcatgtgcat
    10921 agcatcattt ttgcttatca acgacaataa tataagcttc atgttaatca atgtatttgt
    10981 gtttgctgag gctttaatat gcattttata ctatgcttat ctgagtaaaa ttaaggtgaa
    11041 aacttcagtt ttattcttaa tttcaccatt attatgcatg ctaattatta ttgtttcatt
    11101 tttacagcac tatgcttaag gcgatatatg atacgattaa ctattattat agtaatatat
    11161 ggtaaggagt gttttgattc ccgcactctt caaagtttaa agttatgccc tttacaccaa
    11221 aatattacgt tatttatttt taacaatggg ccacggttgc tttctgaaga taaatgttat
    11281 gagttgtttg atgaacaaat aaaaaaaaat aagattaatt tgatactaaa acaaaatatt
    11341 aataatattc ctctgagtgt gatttataat gatgctatca aagaaaatgc tgctagtgat
    11401 tatttcatga tacttgacga tgatacggaa atcccttcaa gctattttgc ttttaatgct
    11461 aatcgttttg atttcgattt atctgttccg ttaataaaaa cgggtgagtc ggaagagttt
    11521 ttttatccaa tagttgatgg aattataccc attaaaaaac aatctgtaga tgattatgag
    11581 gaaatatata caatttcttc cggtttaatg ataagtaagt ctttaacaga aacattcaaa
    11641 tgttacttta atgatgtttt tgatactcga tttgcttttt acggtataga ttttaacttt
    11701 ttcagacgct taagaataat aaaacatcat ggacattatt tccgattgtt tattgctgaa
    11761 cctataagac attctctttc tagatttgat ggtgaacaaa cggaatggag aaatattgag
    11821 cgcatatatg acgtggttct tagcctgaaa tattatgaaa aaagaaatat ggtcaaagtt
    11881 gtcaagcttg ctaggcttgt ttcatctagc ctcctcaaat ttaaatttag aagcataaag
    11941 gctatttttt atagcttatt agttggtatg caccctcgct caaaaaaata tattagtaat
    12001 aagaaaatta taaaataatc tacctaaata ttcaacattg tatatatact aacagaaatt
    12061 aaatatcaga aggttttcga tgaatcatgt tactgctata ttagtaactt acaacccaga
    12121 ttatgagttg ttacctcatg tggtggagca aatcacgcaa caagtagact ctgtcatttt
    12181 gattgataat gcatcaacct gttcagaaaa aattttttca gtaattaaag aatacagtaa
    12241 tatacatttt tattccttaa gtgagaatat cggtttagcg gctgctcaaa atcttgcaat
    12301 atcaaaagta caggaaaagt gtaattatgt gatttttttc gatcaggata gcgtaattca
    12361 aaaggattta attaaacaat tattgattgc tgattttaga ttaaggcaag caggtgaaaa
    12421 tgttgctgct gttggccctt cctttattga tccgactaat aatatgatat accccgcaac
    12481 tgtttataag gggcctttta taaaaaaagt tgatgttacc tcacaaccta ttgaagcaac
    12541 ttttattatt gcatcgggat gtttaatcaa aacttcagca attaatgaga ttggattaat
    12601 gtatgatggt ttctttattg attatattga cgttgaatgg tgcttaagag caaaaagcaa
    12661 ggggtacagt gtttatgtca tacccaatgc aaaaatgtat catactattg gtgatagccg
    12721 tatcactata ttaggaagaa caatttctat tcataacccg atgaggcgat actatttaat
    12781 tcgtaatagt atcttaatat gcaaattaaa atatatacct ataggttata aaattagaga
    12841 gtgtatgttt aacataattc gagccattct ctctttgtta ttaaataata ataaaaaatt
    12901 aacattaaaa atgatttttc gcgggataat tgatggggtt aagggtatta cagggagagt
    12961 ggaaataaaa tgagaaagcg tttagcgcat atacaggttg ttcctaaaat gtctggggtg
    13021 caacaaatat cctttgatat cttaaaaaat cttgatcccg atgaatttga tttgtatttg
    13081 atatgtggtg atggtactga aattgagaac ttcaaaaaaa gatttcaatc tattaatgta
    13141 aaagtaatcg taattccaga attgaaaaga gaaatttgct tttcagatat taaggctttt
    13201 tttcgtttat tagctttatt taaaaggcac aaaattgata ttgttcatac taattctaca
    13261 aaacccggtg tcatagctag gattgcggca aaatttgccg gggtaagaaa aattgttcac
    13321 acagttcacg gaatcgcttt ccatgctgaa atgaacacgt ttaaaagatt gttttattgg
    13381 ctagttgaat atttttcatg cttttttggt gatgtcaatg ttcttgttaa taaacattat
    13441 ttaatgtatt acccattcat taataatatg gttgtttaca atggagttga ttttggacag
    13501 tttaccggta gtaaatctgt cagtgatcaa ttgcactttg cctttatggc aagattagat
    13561 gatcaaaaaa atccctttga atttattgaa gcaattaaaa ttatacatga cacacatcca
    13621 catcttttat ctaacgtgaa atttacttta gctggtgatg gagaattggc tgaacagtgc
    13681 cgttttctga tagtccgata cggacttgag aacaaagtaa atatgcctgg ttggattata
    13741 gacaagaata agttttttag cgatgttgat gttatttgtc agccatcaaa atgggaagca
    13801 tttggattgg tatttgctga agctgcctat tttaaaatcc caagtattgc cagagctgta
    13861 gagggggtgc ccgaagttat tattaatgga aaaacagggt tgctttataa taaatctgaa
    13921 gatgaacttt ccgttttaat gattaaattg ataaataatc cctctctcat atcagaattg
    13981 ggagagcaag cctattctta tgttaccaat aaattttcac ttaatacaat gctagctaat
    14041 tataaacaac tttacacttc ttgagtgtta taattttctt aatgcgttat agaagatcat
    14101 ttacttataa tatatccttc tattaggggc ataatttaaa taaatgcccg ataaccatat
    14161 ccacatcctt gtggtgaagc accccctgac aggagtaaac aatgtcaaag caacagatcg
    14221 gcgtcgtcgg tatggcagtg atggggcgca accttgcgct caacatcgaa agccgtggtt
    14281 ataccgtctc tattttcaac cgttcccgtg agaaaacgga agaagtgatt gccgaaaatc
    14341 caggcaagaa actggttcct tactatacgg tgaaagagtt tgttgaatct ctggaaacgc
    14401 ctcgtcgcat cctgttaatg gtgaaagcag gtgcaggcac ggatgctgct attgattctc
    14461 tcaagccata cctcgataaa ggcgacatca tcattgatgg tggtaacacc ttcttccagg
    14521 acaccattcg tcgtaatcgt gagctttctg cagaaggctt taacttcatt ggtaccggtg
    14581 tttccggcgg tgaagaaggt gcgctgaaag gtccttccat tatgcctggt gggcagaaag
    14641 aagcctatga actggttgca ccgatcctga acaaaatcgc cgcagtggct gaagacggtg
    14701 agccatgcgt tacctatatt ggtgccgatg gcgcaggtca ctatgtgaag atggttcaca
    14761 acggtattga atacggcgat atgcagttga ttgctgaagc ctattctctg cttaaaggtg
    14821 gcctgaatct ttccaacgaa gaactggcgc agacgtttac cgagtggaat aacggtgaac
    14881 tgagcagcta cctgatcgac atcaccaaag atatcttcac caaaaaagat gaagacggta
    14941 actacctggt tgatgtgatt ctggatgaag cagcaaataa aggtacgggc aaatggacca
    15001 gccagagtgc gctggatctc ggcgaaccgc tgtcgctgat taccgagtct gtgtttgcac
    15061 gttatatctc ttctctgaaa gatcagcgcg ttgccgcgtc taaagttctc tctggcccgc
    15121 aagcgcagcc agctggcgac aaggctgagt tcatcgaaaa agttcgtcgt gctctgtatc
    15181 tgggcaaaat cgtttcttac gcccaaggct tctctcagct gcgtgctgcg tctgaagagt
    15241 acaactggga tctgaactac ggcgaaatcg cgaagatttt ccgtgctggc tgcatcatcc
    15301 gtgcgcagtt cctgcagaaa atcactgatg catatgccga aaatccgcag atcgctaacc
    15361 tgctgctggc tccgtacttt aaacaaattg ccgatgatta ccagcaggcg ctgcgtgatg
    15421 tcgttgctta tgcagtacag aacggtatcc cggttccgac cttcgccgct gcggttgcct
    15481 attatgacag ctaccgtgcc gctgttctgc ctgcgaacct gatccaggca cagcgtgact
    15541 atttcggtgc gcatacttat aagcgcattg ataaagaagg tgtgttccat actgaatggc
    15601 tggattaatc tgatttaaat caattaacca acacaaggcc cggagaaacc ctccgggctt
    15661 ttttattata caaagcggca ggttatggcc tttttttata atttatgatt aaaaaagcga
    15721 tataatacag agccgcacag caggatcgct gtcttgacag ttcaaccgca tcagcgttaa
    15781 aaatcccgca gtagatgaag ttgtggtggt ggactaatga ccactctaaa tgtttaaccg
    15841 gaagaagtca gagctaatga aaataacaat ttcaggaaca ggttatgttg gtctttcaaa
    15901 tggtattctg attgcgcaaa accacgaagt ggttgcactg gatatcgttc aggccaaagt
    15961 ggacatgctt aacaagaagc agtcaccgat tgttgataag gagattgaag agtatctggc
    16021 gactaaagag ctcaatttcc gcgctacgac agataagtat gacgcgtata aaaatgccga
    16081 ttacgttatt attgccacac ctaccgatta tgatccgaaa acgaattatt ttaatacctc
    16141 aagcgtggaa gcggtcattc gcgatgtgac aaaaattaac cctaatgctg tcatggtgat
    16201 taaatctaca attccggtag gtttcaccaa atcaattaaa gaacgtctgg gtatcgataa
    16261 ccttattttc tctccggaat tcctccgcga aggtaaagct ctttacgata accttcatcc
    16321 gtcacgtatt gtaatcggtg agcgttcgga acgcgcagaa cgttttgctg cgttattaca
    16381 ggaaggcgcg attaagcaaa atatcccgac cctgttcacc gattcaaccg aagcagaagc
    16441 gattaaactg tttgctaata cctatttggc gatgcgcgta gcatacttta atgaactgga
    16501 tagctatgca gaaagtttag gtctgaatac tcgccagatt atcgaaggcg tttgtctcga
    16561 tccgcgtatt ggcaaccatt acaacaatcc gtcgtttggt tatggtggtt attgtctgcc
    16621 gaaagatacc aagcagttac tggcgaacta ccagtctgtg ccgaataacc tgatttcggc
    16681 aattgtcgat gctaaccgca cacgtaaaga ctttattgcc gatgccattt tgtcacgcaa
    16741 accgcaagtg gtgggtattt atcgtctgat tatgaagagc gggtcagata actttcgcgc
    16801 gtcttccatt caggggatta tgaagcgtat caaggcgaaa ggcgttgaag tgatcatata
    16861 cgagccagtg atgaaagaag actcattctt caactctcgc ctggaacgag atctcgccac
    16921 cttcaaacaa caagccgacg tcattatttc caaccgtatg gcagaagagc ttagggatgt
    16981 ggcagataag gtatacaccc gcgatctctt tggcagcgac taacatcctg ttatcagggc
    17041 gattttcgcc ctgattctct tatgttccct ttgtaataat tcattatttt tatcatttat
    17101 cctatagcat tcatggcgat tatcgctaaa ctatggcgac ttaaaattat ttcgtccgtt
    17161 agggtaatga tgagagtaga aaataataat gtttctgggc aaaaccatga cccggaacag
    17221 attgatttga ttgatttact ggtgcagttg tggcgtggca agatgacaat tatcatctct
    17281 gtcattgttg ctattgccct ggctataggg tatctggccg ttgccaaaga aaagtggact
    17341 tccacagcta tcgttactca acctgatgtg gggcaaatcg ctggttatac caacgcgatg
    17401 aatgtgatct acggatcggc tgtaccgaaa gtctccgaca ttcaggcttc acttatcgga
    17461 cgttacagca ctgcattctc agcattagcg gaaacgctgg ataaccagga agaagcagag
    17521 aagctgacca ttgagcctac cgttaaaaac cagccgttac cactggcggt gtcttatgtg
    17581 ggtgaaacac ctgaaggtgc gcagaagcag ctggcgaagt atatccagca agttgatgac
    17641 caggtaaacg aagaactgga aaaagacctg aaagacaaca tcgcgctgcg tatgaaaaac
    17701 ttgcaggatt ctctgaaaac tcaggaagta gtcgcgcagg agcagaaaga tctgcgtatc
    17761 cgtcagattc aggaagcgtt gcagtatgcg aatcaggcgc aggtgacaaa gccgcagatt
    17821 cagcagacac aggacgtaac ccaagatact atgttccttt tggggagcga agcgctggag
    17881 tcgatgatta agcatgaggc gactcgtccg ttggtgttct caccaaacta ctatcagaca
    17941 cgtcaaaacc tgttggatat tgaaaactta aaggttgatg atcttgatat tcatgcttac
    18001 cgttatgtga tgaaaccgac gttacctatt cgtcgcgata gcccgaaaaa ggcaattacc
    18061 ttgattctgg cggtgctgct gggcggcatg gttggcgcgg ggattgtgct ggggcgtaac
    18121 gctctgcgta attacaacgc gaagtaatct tttcggtttt aaggaaaaag ggcagggtgg
    18181 tgacaccttg cccgtttttt ttgccggatg cgacaacaac atcgcatccg atcacccgca
    18241 actcactgat gccgtttacg caggttctca attaccgtcg ttaaatccag atcctgatcc
    18301 tgcaacaata ccagcaggtg atacatcaag tcagacgcct cgttggttag ctcaaagcgg
    18361 tcatgtaccg tcgcggccag tgcggtttcc accccttctt cgcccacttt ctgcgcaatg
    18421 cgtttggtgc cgctggcata cagtttggct gtgtaagagg tttccgggtc ggcagattta
    18481 cgttcggcga gcagttgttc cagttgatac aggaacagcc attggtgagc ggtgtcgccg
    18541 aagcagctgc tggtgccttt gtggcaggtt ggcccgatgg gattcgccag caccagtaac
    18601 gtgtcgttgt cgcagtccgg ggcaatactc accacattga gaaaattgcc cgacgtttcg
    18661 cctttggtcc acagtcgctg tttagtgcgc gagaagaagg tgactttgcc gctttcgatg
    18721 gttttgtcta aggcttccgg gttcatatag cccagcatta acacttcacc ggataccgcg
    18781 tgttgcacaa tcaccggcat aagtccgtcg gttttttccc agtccagttc gcgacgttgt
    18841 tgttctgtta acatatcctg atctccacgc cctgtgttgc caggtacgct tttaattcac
    18901 caatattgat tatttgtttg tggaatacag aagctgccag cgcaccgtca acgtcggcat
    18961 cgcggaaggc ttcgaggaag tgttccatgg tgcccgcgcc accggaagca atcagcggga
    19021 cgtggcaaac ttcacgcact tttttcagct gctctaggtc gtaaccgtta cgcacgccgt
    19081 cctgattcat catgttaagg acgatttctc cggcaccgcg tttttgcact tcctgtaccc
    19141 agtcgagcgt ttcccactga gtgacgcggg tgcggctttc atcgccggta tattgattca
    19201 catgatattt accggtttca gcgtcgtacc aggtat
//
