LOCUS       LC550086               20282 bp    DNA     linear   BCT 04-AUG-2020
DEFINITION  Escherichia coli OT-571 genes for O-antigen biosynthesis gene
            cluster, complete cds.
ACCESSION   LC550086
VERSION     LC550086.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S. and Lee,K.
  TITLE     Additional O-genotyping PCR primers targeting novel E. coli and
            Shigella-unique O-genotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20282)
  AUTHORS   Iguchi,A., Iyoda,S. and Lee,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2020) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuenkibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..20282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="OT-571"
                     /db_xref="taxon:562"
                     /note="OgN34 O-antigen biosynthesis gene cluster"
     gene            420..1814
                     /gene="wcaM"
     CDS             420..1814
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BCG06378.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARPSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGKGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLTMTGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHIIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGILIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSINFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            1972..2967
                     /gene="gne"
     CDS             1972..2967
                     /gene="gnu"
                     /note="OgN34_1"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BCG06379.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            3210..4103
                     /gene="galF"
     CDS             3210..4103
                     /gene="galF"
                     /note="OgN34_2"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BCG06380.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            4667..5560
                     /gene="rmlB"
     CDS             4667..5560
                     /gene="rmlB"
                     /note="OgN34_3"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BCG06381.1"
                     /translation="MARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLL
                     EAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNIKEGLPLFTETTAYAPSSP
                     YSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKGLPIY
                     GKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVP
                     KEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWYLSNTKW
                     VDNVKSGAYQSWIEQNYEGRK"
     gene            5560..6459
                     /gene="rmlD"
     CDS             5560..6459
                     /gene="rmlD"
                     /note="OgN34_4"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="BCG06382.1"
                     /translation="MNILLFGKTGQVGWELQRSLAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHF
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRMALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLKAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWRVGVKRMLNELFTTTAI"
     gene            6517..7392
                     /gene="rmlA"
     CDS             6517..7392
                     /gene="rmlA"
                     /note="OgN34_5"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BCG06383.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAKQVRKLAESL
                     SKNAYGRYLYTMLDV"
     CDS             7446..8237
                     /note="OgN34_6"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="glycosyltransferase"
                     /protein_id="BCG06384.1"
                     /translation="MSLYDKEKPEYLDQCLNSLSRQTLLADEIVMVYDGPINIHLESV
                     VDKWSRLLPIKIVKLEKNVGLGKALNEGLINCTFDIVARMDTDDICNEKRFEKQINMF
                     ARDDELSILGSYIEEFEGAEKNIISVRHVPQTYNEILNTIKLKNPFNHMTVMFKKKAI
                     LSVGGYQKHHLMEDYNLWLRLISKGYKGLNIPENLVSVRAGRDMIARRHGVKYIGSEL
                     KLAKLKMELGYLGVTRSIYIFILRSLPRLMPIFILKEIYKIARVK"
     gene            8256..8813
                     /gene="rmlC"
     CDS             8256..8813
                     /gene="rmlC"
                     /note="OgN34_7"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="BCG06385.1"
                     /translation="MKVIKTEIENVLIFEPAIFEDDRGFFYESYNKKKFTELTGIVED
                     FVQDNHSKSIQGVIRGLHFQKEPCAQGKLVRCIRGSVFDVIVDIRPQSETYGKWIGIE
                     LTEKNKRQIWIPAGFAHGFQTLSLEAEFLYKTTDYYSPKNEKCIIWNDETLNINWPLD
                     NVILSEKDAEGLTLKEWQQEMAYGN"
     CDS             8803..9204
                     /note="OgN34_8"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="NDP-hexose isomerase"
                     /protein_id="BCG06386.1"
                     /translation="MEIKFIPLQKHGDDRGSLVALEEENNIPFTIKRVYYLFNTKRNV
                     VRGLHAHKKLMQVAIAVRGSCRFLLDDGKERIEILLDNPAQGLLIESFMWREMYDFSD
                     DCVLMILADSHYDEDDYVRNYDDFIQLAHKK"
     CDS             9224..10327
                     /note="OgN34_9"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="putative aminotransferase"
                     /protein_id="BCG06387.1"
                     /translation="MINFLNLKTLNQKYSQELNEACKRVIDSGWYIMGDELESFERNF
                     AEYCGTRHCIGVANGLDALTLSLKTWKILGKLKDGDEIIVQANTYIASILAITANNLK
                     PVLVEPSIESYNLSKQNILDAITEKTKAILPVHLYGQISPMEDIMSIAKKYDLIVLED
                     SAQAHGASINGKRAGGWGNASGFSFYPGKNLGALGDAGAITTNCDEFNDVIKALRNYG
                     SNKKYHNLYDGVNSRLDEIQAAILNVKLKYLDNETNIRREIANYYINNIDNKNIIIPS
                     LKNEENHVWHLFVIRCKKRELLIKHLIENGVQTLIHYPIPPHKQNCFRSWNNLVLPIT
                     EMIHDEVLSIPLDSTLTQKDVERIVSSINSFKS"
     gene            10324..11574
                     /gene="wzx"
     CDS             10324..11574
                     /gene="wzx"
                     /note="OgN34_10"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BCG06388.1"
                     /translation="MKKILSISLLSGIMTLIKMVMGFAISKIVAVYAGTSGVALLGQM
                     QSVILSLNGIINSPVGNGVIKFTSENSHDFEKCSPYWRASILYVMCFSTIIIIVGCFY
                     AQEISLLLFGHSDNYWIVLLMVFVAPITSIGTLINSILNGLESYKQYVYTGIIASVLS
                     GLIMIFVIVNFGIVGALVGASLQYALIGIISFIINIYQPWLKIGYLIGKISTDVKKNI
                     AEYIFMAIVSAVSLPLGMILIRGFLIEYTGWKNAGEWQAVWKISEAYLSVITMGLSVY
                     YLPRLSSLYDHNSLVREVKSVASIVLPIVSLMAVAVYILRDFSISLLYTEQFREAREL
                     FKIQLIGDVIKVISWVYAYPMIAKKSTKLFVFTEIVFTITLVLLSYFFIKKYGVHGAN
                     LAYVLNYILYFICVYILLNKAIKN"
     CDS             11820..12512
                     /note="OgN34_11"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf12"
                     /product="glycosyltranslocase"
                     /protein_id="BCG06389.1"
                     /translation="MSLKLIDTEYAFFIASDDIINPYGFTSMFQTMLAHENMNIAIGN
                     AYVLYTGKTPEQKVYGKEHFDFFSKKDSEIRKDIFINFPKPLLLQSTIFKTACLRDVG
                     GWDSKLVWDDYPMFVKLFMRNSFDRGNIFFFADFVVSFYRQHQFNTYKNLLKQLSMVE
                     ETFKSIVPLKYYKDAISNQYAFYFLLSLREKNNKAIKKIIKVIVCEKLFFYFAKYFFV
                     HIIKWISRRLSK"
     gene            12881..13813
                     /gene="wzy"
     CDS             12881..13813
                     /gene="wzy"
                     /note="OgN34_12"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BCG06390.1"
                     /translation="MLLIYLSKVSIAGLLLSRFDANKGLGPIVRIMDALRLIIAAWMY
                     INYIRLGKKKYLIIALVFSCVSAFFSGAKFALLEQMYVMFVAGFVGERIKIKLHLKTI
                     VLAGVFLVALFLFILFMLQKLSDGIGYQNSQYIPGAPVTLELFILRILANGDSYYLSL
                     TQRVLDDININFPVLQFFSNVFGNSFMSRLFGQDFSNGDIGRQIWLYWYPDDDIMRGP
                     TAHFDISGLVYFGYIGGVIFSIVIGVVLGSLNKLKYSCYHAPTVIVAFVSALYCRSLP
                     LMLNPSVGLAYIVDIYMLLFLLLVLSTLSRKRIG"
     CDS             13813..14670
                     /note="OgN34_13"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf14"
                     /product="glycosyltranslocase"
                     /protein_id="BCG06391.1"
                     /translation="MSVLAVIVTYNSDLQKLKTIFNIVCIDADIIVSDNSTEQNTRER
                     IQELCEIHSVKYISMGGNVGIAKAQNDGIKLGLNMGYEDILLLDDDSLPHPTMISSLL
                     IVRGFVTENIKCLPVICANPCDAKGNSLAKYDKKLFDFIYNCRDVTSSGTLINKSHFD
                     LVGLHDEKLFIDCVDFEWGWRAISKGVQIVLSQKAILTHQLGTGRLNIINAGYGAPIR
                     HYYQFRNVLNMCIREYTPFPWKISQIFKLGIKYFILIPFFFDNKVLRLKYATRGVLSF
                     IRGKYGPIN"
     CDS             14695..15243
                     /note="OgN34_14"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf15"
                     /product="putative N-acetyltransferase"
                     /protein_id="BCG06392.1"
                     /translation="MKIKAKIIYGYTIDLVNACENDAEFILSLRTNISKSRFLSKTST
                     VLEDQRKWLATYSRDNKQAYFIIKNKRGEDIGTVRIYDLQGLSFCWGSWILSDKAHSN
                     ASIESALIIYQYALMRGFKSAHFDVRKGNKSVCKFHERFGAFKVGETKDDFLYDIDED
                     NIIQSLHRYKRFLPNSIVFEEM"
     gene            15327..16733
                     /gene="gnd"
     CDS             15327..16733
                     /gene="gnd"
                     /note="OgN34_15"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BCG06393.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            16982..18148
                     /gene="ugd"
     CDS             16982..18148
                     /gene="ugd"
                     /note="OgN34_16"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BCG06394.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQADKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVVKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFITDAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            18238..19272
                     /gene="wzz"
     CDS             18238..19272
                     /gene="wzz"
                     /note="OgN34_17"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BCG06395.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETA
                     EGAQKQLAQYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(19369..19980)
                     /gene="hisI"
     CDS             complement(19369..19980)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BCG06396.1"
                     /translation="MLTELQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVNDRFELKNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     5998 a   3798 c   4501 g   5985 t
ORIGIN
        1 catcgaacaa tatcaactgg aagatgtggt agatatgccg ggctttaaac cgagccacga
       61 agtgaaagcg atgctcgacg acgcggatgt cttcctgttg ccatcggtaa cgggcgcgga
      121 tggcgatatg gaaggcattc cggtagcgct gatggaagcg atggcggtcg gtattccagt
      181 agtttccact ctgcatagcg gaataccgga actggtggag gccgataaat ccggctggct
      241 ggtgcctgag aacgatgctc gtgcactggc gcaacgactg gcggcgttta gccaactgga
      301 caccgacgaa ttggctccgg tcgtcaaacg cgcacgcgaa aaagtcgaac acgattttaa
      361 ccagcaggtg attaaccgag aactcgccag cttgctgcag gctttataga ggagaacgta
      421 tgccatttaa aaaactctcc cgacgcacct tcctgacggc aagctcggcg cttgccttcc
      481 tccatacccc tttcgctcgc gcacttcctg ctcgaccaag cgttaatatc aacgattaca
      541 atccgcacga ctggatcgcc tcatttaaac aagcttttag tgaagggcaa acggtcgtcg
      601 tgcctgccgg attggtttgt gacaatatca acaccggcat cttcattcct gcaggtaaaa
      661 cgttacacat ccttggaagc ctgcgcggca acggcaaagg gcgatttgtt ttacaggacg
      721 gcagccaggt gacaggggag gagggcggta gtatgcataa catcaccctg gatgtgcgtg
      781 gttctgactg caccatcaaa gggctgacga tgaccggctt tggcccggtg acgcagatct
      841 atatcggcgg caaaaacaaa cgggtcatgc gtaacttgac catcgataac ctcactgtca
      901 gccacgctaa ttacgccatc ttacgccagg gatttcataa ccagattatc ggtgccaaca
      961 tcaccaactg taagttcagc gacttacaag gcgacgccat tgaatggaat gtggcgatta
     1021 acgaccgcga tattttgata tccgaccaca tcatcgagcg catcaactgt accaacggca
     1081 aaatcaactg gggcatcggc ataggccttg cgggaagcac ttacgataat aattacccgg
     1141 aagatcaggc agtgaaaaac tttgtcgtgg cgaatatcac gggatcggat tgtcgacagt
     1201 tgatccatgt tgaaaatggt aaacattttg ttattcgtaa tatcaaagcc cgcaatatca
     1261 cgccggattt cagtaagaaa gcaggcattg ataacgccac ggtcgctatt tacggttgtg
     1321 acaatttcgt gattgataat attgaaatga ttaatagcgc cgggatttta attggctatg
     1381 gggtaattaa aggcaaatat ctctcgatac cgcaaaattt ccgagtgaat aatattcagc
     1441 tggataacac ccaccttgct tacaaattgc gcggcatcca aatctctgcc gggaatgccg
     1501 tctcctttgt ggcactgact aacattgaga tgaagcgtgc ctcgctggag cttcacaata
     1561 aaccgcaaca tctttttatg cgtaatatca atgtgatgca ggaatcctca gttggacccg
     1621 cattgagcat aaacttcgac atgcgcaaag acgttcgagg cgtttttatg gcgaaaaaag
     1681 aaacactgct gtcacttgca aatgttcatg cggtgaatga aagagggcaa agctccgtcg
     1741 atatcgacag gattaatcac catattgtta atgtggaaaa gattaacttt agattgccgg
     1801 aacggagaga gtagatttgc gaccattcat aaacttactg gatataatgt tctaaaagat
     1861 tataatcctc acactaactg ttaaagctgg ggctgggcag cattgactac tcagggaagg
     1921 tactcatctt tatcttgtgt ttctttcgta acaaccagac taaagtgatt tatgaacgat
     1981 aacgttttgc tcataggagc ttccggattc gtaggaaccc gactacttga aacggcaatt
     2041 gctgacttta atatcaagaa cctggacaaa cagcagagcc acttttatcc agaaatcaca
     2101 cagattggcg atgttcgtga tcaacaggca ctcgaccagg cgttagccgg ttttgacact
     2161 gttgtactac tggcagcgga acaccgcgat gacgtcagcc ctacttctct ctattatgat
     2221 gtcaacgttc agggtacccg caatgtgctg gcggccatgg aaaaaaatgg cgttaaaaat
     2281 atcatcttta ccagttccgt tgctgtttat ggtttgaaca aacacaaccc tgacgaaaac
     2341 catccacacg atcctttcaa ccactacggc aaaagtaagt ggcaggcaga ggaagtgctg
     2401 cgtgaatggt ataacaaagc accaacagaa cgttcattaa ctatcatccg tcctaccgtt
     2461 atcttcggtg aacgcaaccg cggtaacgtc tataacttgc tgaaacagat cgctggcggc
     2521 aagtttatga tggtgggtgc aggaactaac tataagtcca tggcttatgt tggaaacatt
     2581 gttgagttta tcaagtacaa actgaagaat gttgccgcag gttatgaggt ttataactac
     2641 gttgataagc cagatctgaa catgaaccag ttggttgcgg aagttgaaca aagcctgaac
     2701 aaaaagatcc cttctatgca cttgccttac ccactaggaa tgctgggtgg atattgcttt
     2761 gatatcctga gcaaaattac gggcaaaaaa tacgcagtca gctctgtgcg cgtgaaaaaa
     2821 ttctgcgcaa caacacagtt tgacgcaacg aaagtgcatt cttcaggttt tgtggcaccg
     2881 tatacgctgt cgcaaggtct ggatcgaact ctgcagtatg aattcgtcca tgccaaaaaa
     2941 gacgacataa cgtttgtttc tgagtaatgc aggcgagtca gagaaaaaag gaccaggttg
     3001 gactacgatt attcggaaag aggtactgat taaattatca gtatgcggaa ttccgagtaa
     3061 tccgagtaac ccagatgctg atggtgtgta gtgttatcgt aaggtttaat caaagttatg
     3121 cggaactaag ctttggtata tagccataac gtttggctaa ccaatggcaa taatttacgc
     3181 actatcagcc acttagaagt aggaaaagaa tgacgaattt aaaagcagtt ataccggtag
     3241 caggtcttgg gatgcatatg ttgcctgcca ctaaggcgat tcccaaagag atgctaccga
     3301 tcgtcgacaa gccaatgatt cagtacattg ttgacgagat tgttgctgca gggatcaaag
     3361 aaatcctcct ggtaactcac gcgtccaaga acgcagtcga aaaccacttc gacacctctt
     3421 atgaattaga atctctcctg gaacagcgcg tgaagcgtca actactggcg gaagttcagt
     3481 ccatctgccc gccgggcgtg accattatga acgtgcgtca gggcgaacct ttaggtttgg
     3541 gccactccat tttatgtgcg cgacctgcca ttggtgacaa cccatttgtc gtggtgctgc
     3601 cggacgttgt tatcgatgat gccagcgccg acccgttgcg ttacaacctt gctgccatga
     3661 ttgcgcgctt caatgaaacg ggacgcagcc aggtgctggc aaaacgtatg ccgggtgacc
     3721 tctctgaata ctccgtcatc cagaccaaag aaccgctgga ccgtgaaggc aaagtcagcc
     3781 gcattgttga attcatcgaa aaaccggatc agccacaaac gctggactca gacatcatgg
     3841 ccgtgggtcg ctatgtgctt tctgccgata tttggccgga acttgaacgc actcaacctg
     3901 gtgcatgggg gcgtattcag ctgactgatg ccattgctga actggcgaaa aaacagtccg
     3961 ttgatgcaat gctgatgact ggtgacagct acgactgcgg taaaaaaatg ggctatatgc
     4021 aggcgtttgt gaagtatggc ttacgcaacc tgaaagaagg ggcgaagttc cgtaaaggta
     4081 ttgagaagct gttaagcgaa taatgaaaat ctgaccggat gtaacggttg ataagaaaat
     4141 tataacggca gtgaagattc gtggcaaaag taatttgttg agaatattcc tgccgttgtt
     4201 ttatataaac aatcagaata acaacgagtt agcaatagga ttttagtcaa agttttccag
     4261 gattttcctt gtttccagag cggattggta agacaattag cgtttgaatt tttcgagtta
     4321 agcgcgagtg ggtaacgctc gtcacatcgt aggcatgcat gcagtgctct ggtagctgta
     4381 aagccagggg cggtagcgtg cattaatacc tctattaatc aaactaagag ccgctaattt
     4441 cacagcatgc tctgaagtaa tatggaataa aaaagtgaag atacttgtta ctggtggcgc
     4501 aggatttatt ggttctgctg tagttcgtca cattataaat aataggcagg atagtgttgt
     4561 taatgtcgat aaattaacgt acgccggaaa cctggaatca cttgctgatg tttctgattc
     4621 tgaacgctat gtttttgaac atgcggatat ttgcgatgct gctgcaatgg cgcggatttt
     4681 tgctcagcat cagccggatg cagtgatgca cctggctgct gaaagccatg ttgaccgttc
     4741 aattacaggt cctgcggcat ttattgaaac caatattgtt ggtacttatg tccttttaga
     4801 agccgctcgt aattactggt ctgctcttga tagcgacaag aaaaatagct tccgttttca
     4861 tcatatttct actgacgaag tctatggtga tttgcctcat cctgacgagg taaatattaa
     4921 agaaggatta cccttattta ctgagacaac agcttacgcg ccaagcagcc cttattccgc
     4981 atccaaagca tccagcgatc atttagtccg cgcgtggaaa cgtacctatg gtttaccgac
     5041 cattgtgact aattgctcta acaattatgg tccttatcat ttcccggaaa aattgattcc
     5101 attggttatt ctcaatgctc tggaaggtaa aggattacct atttatggta aaggggatca
     5161 aattcgcgac tggctgtatg ttgaagatca tgcgcgtgcg ttatataccg tcgtaaccga
     5221 aggtaaagcg ggtgaaactt ataacattgg tggacacaac gaaaagaaaa acatcgatgt
     5281 agtgctcact atttgtgatt tgttggatga gattgtcccg aaagagaaat cttaccgcga
     5341 gcaaattact tatgttaccg atcgtccggg acacgatcgc cgttatgcga ttgatgctga
     5401 gaagattggt cgcgaattgg gatggaaacc acaggaaacg tttgagagcg ggattcggaa
     5461 gacagtggaa tggtacctgt ccaatacaaa atgggttgat aatgtgaaaa gtggtgccta
     5521 tcaatcgtgg attgaacaga actatgaggg ccgcaagtaa tgaatatcct ccttttcggc
     5581 aaaacagggc aggtaggttg ggaactacag cgttctcttg ctcctctggg taacttgatt
     5641 gctcttgatg ttcactccac tgattattgt ggcgatttca gcaacccaga aggtgtggct
     5701 gaaaccgtca aaaaaattcg cccagatgtt attgttaatg ctgcggctca taccgcagta
     5761 gataaagctg agtcagaacc cgaatttgca caattactca atgcgaccag cgttgaatca
     5821 attgcaaaag cggctaatga agttggggcc tgggtaattc atttctcaac tgactacgta
     5881 ttccctggaa atggcgacac tccatggctg gagactgatg caaccgcacc actaaatgtt
     5941 tacggtgaaa ccaagttagc tggagaaaaa gcattacaag agcattgtgc gaagcacctt
     6001 atttttcgta ccagctgggt atatgcaggt aaaggaaaca attttgccaa gacaatgtta
     6061 cgtctggcaa aagagcgcga agaattagct gtcattaacg atcagtttgg tgcaccaaca
     6121 ggtgctgaac tgctggctga ttgtacggca catgcaattc gtatggcact gaataaacca
     6181 gaagtcgcag gcttgtacca tctggtagcc agtggtacca caacctggca cgattatgct
     6241 gcgctggttt ttgaagaggc acgaaaagca ggtattcccc ttgcactcaa caagctcaag
     6301 gcagtaccaa caacagccta tcctacacca gctcgtcgtc cacataactc tcgccttaat
     6361 acagaaaaat ttcagcagaa ttttgcgctt gtcttgcctg actggcgggt tggcgtgaaa
     6421 cgaatgctca acgaattatt tacgactaca gcaatttaat agtttttgca tcttgttcgt
     6481 gatggtggag caagatgaat taaaaggaat gatgaaatga aaacgcgtaa aggtattatt
     6541 ttagcgggtg gttctggtac tcgtctttat cctgtgacta tggctgtcag taaacagcta
     6601 ttacctattt atgataagcc gatgatctat tacccgctct ctacactgat gttggcgggt
     6661 attcgcgata ttctgattat tagtacgcca caggatactc ctcgttttca acaactgctg
     6721 ggtgacggta gccagtgggg gctaaatctt cagtacaaag tgcaaccgac tccagatggg
     6781 cttgcgcagg cgtttattat cggtgaagag tttatcggtg gtgatgattg tgctttggtt
     6841 cttggtgata atatcttcta cggtcatgat ctgccgaagt taatggatgt cgctgttaac
     6901 aaagaaagtg gtgcaacggt atttgcctat cacgttaatg atcctgaacg ctacggtgtc
     6961 gttgagtttg ataaaaacgg tacggcgatc agcctggaag aaaaaccgct acagccaaaa
     7021 agtaattatg cggtgacagg gctttatttc tatgataacg acgttgtcga aatggcgaaa
     7081 aaccttaagc catctgcacg tggtgaactg gaaattaccg atattaaccg tatttatatg
     7141 gaacaagggc gtttgtctgt tgctatgatg ggccgtggtt atgcatggct ggacacgggg
     7201 acacatcaaa gtcttattga agcaagcaac ttcattgcaa caattgaaga acgtcaggga
     7261 ctgaaagttt cctgcccgga agaaattgct taccgtaaag ggtttataga tgcgaaacaa
     7321 gtgagaaagc tagcggaatc tctatcaaaa aatgcatatg gcagatatct ttatacaatg
     7381 cttgatgtat aaattaacat ttgtgataaa caaaaagata gaggtgaaat ttgtttagtg
     7441 ttttgatgtc tttatatgat aaagaaaagc cagaatattt ggatcaatgt ttgaatagcc
     7501 tttcaaggca aacgttgtta gcagatgaaa ttgtgatggt gtatgatggt cctattaata
     7561 tacacttaga aagtgttgta gataaatggt ccagactatt gcccataaaa attgttaagc
     7621 tcgagaagaa tgtgggcttg ggcaaagctt taaacgaagg attaatcaac tgtacttttg
     7681 atatagtcgc acgaatggat acagacgata tatgcaatga aaaaagattt gaaaaacaga
     7741 ttaatatgtt tgctagggat gatgaattat caatacttgg aagttatatt gaagagttcg
     7801 aaggggctga aaaaaatata atttctgtac gtcatgttcc acagacatat aatgaaatat
     7861 taaatactat aaaattaaaa aaccccttca atcatatgac tgtaatgttt aaaaaaaaag
     7921 ccattctgtc agtaggtgga tatcaaaaac atcatctaat ggaggactat aatttatggt
     7981 taaggttaat atcaaaggga tataaaggat taaacatacc cgagaattta gtttctgttc
     8041 gtgctggacg cgatatgatt gcccgacgac atggagttaa atatatagga agcgagctaa
     8101 agctggcaaa attaaaaatg gaactcgggt atttaggtgt cactaggagt atatatatat
     8161 ttatattaag aagtttaccg agactgatgc caatatttat tttgaaagag atatataaga
     8221 tagcaagggt aaagtaatta tttggagtat taaaaatgaa agttataaaa actgaaattg
     8281 aaaatgtact tatatttgaa cctgctattt ttgaagatga tagaggtttt ttttatgaaa
     8341 gctataacaa aaaaaagttt acagagctca ctggcattgt tgaggatttt gtacaggata
     8401 atcattctaa atcgattcaa ggggtgattc gtggattaca ttttcaaaag gaaccttgtg
     8461 cccaaggaaa gttagtgaga tgtattagag gaagtgtatt tgatgtgatt gtagatataa
     8521 gaccacaatc tgagacctat ggtaaatgga taggtattga attgacagaa aaaaataaaa
     8581 ggcaaatatg gatcccggca ggatttgccc atgggtttca aacattaagc cttgaagctg
     8641 aatttttata taaaactact gattactatt ctccaaagaa tgaaaaatgc attatttgga
     8701 atgacgaaac actaaacatt aactggccgt tagataatgt aattttatct gaaaaagatg
     8761 cagaaggttt aactttaaaa gaatggcagc aagagatggc atatggaaat taaatttatt
     8821 ccacttcaaa aacatggcga tgatagaggt tcattagttg cccttgaaga agaaaataat
     8881 atcccattta caatcaaacg tgtttactat ttatttaata ctaaaagaaa tgttgtaaga
     8941 ggattacatg cccataaaaa gttgatgcaa gttgcaattg cagtgagagg ctcttgtcgc
     9001 tttctgctag atgatggaaa agaaagaatt gaaatactct tagataatcc tgcccaaggt
     9061 ttacttattg aatccttcat gtggcgtgag atgtatgact tttcggatga ttgcgtatta
     9121 atgatattag ccgatagcca ttacgatgaa gatgattatg taagaaatta tgatgatttt
     9181 attcagttag cccacaaaaa ataaaaagat attaggagta aggatgatta attttttaaa
     9241 tcttaaaaca ttgaatcaaa agtatagcca agaattaaat gaagcttgta aaagagtaat
     9301 tgattcgggc tggtatatta tgggggatga gcttgaatcg tttgaaagga actttgcaga
     9361 atattgtggg actcgccatt gtattggtgt ggctaacggc ttagatgcat taacactttc
     9421 acttaagact tggaagattt tgggtaaact gaaggatggt gacgagatca ttgttcaggc
     9481 taacacatat atagcctcta ttttggcgat tactgcaaat aatttaaaac ctgttttggt
     9541 tgaaccctca atagaaagtt ataacctcag caagcaaaac atacttgatg cgataactga
     9601 aaaaacaaag gctattttac ctgtacattt atatgggcaa atatcaccaa tggaagatat
     9661 tatgtccatt gcaaaaaaat atgatcttat agtactcgaa gattctgcac aggcacatgg
     9721 ggcatctatc aatggcaaac gagcaggggg ctggggaaat gcctctggtt ttagttttta
     9781 tccaggaaaa aatttgggcg ctctaggtga tgccggtgca ataactacaa attgcgatga
     9841 attcaatgat gtgatcaagg cgctgcgaaa ctatggttct aataaaaaat atcataactt
     9901 atatgatgga gtgaatagtc gtcttgatga aatacaagcg gcaatactaa acgttaaact
     9961 gaaatactta gataatgaaa ctaatattcg acgggaaatt gctaattatt acatcaataa
    10021 tattgataat aaaaatatta tcataccgtc tttaaaaaac gaagaaaatc acgtttggca
    10081 tttattcgtt attcgatgta aaaaaagaga gttactgatt aaacatttaa ttgaaaatgg
    10141 ggttcaaaca ttaattcatt atcccattcc acctcataag caaaattgct ttaggtcttg
    10201 gaataattta gttctcccta ttactgagat gattcatgat gaagttctgt ctattccatt
    10261 agattcaaca ttaacacaaa aggatgtcga aagaatagtt tcttcaataa attcttttaa
    10321 atcatgaaaa aaatactaag catttcttta ctttccggta tcatgacact tataaaaatg
    10381 gttatggggt ttgctatttc aaaaatagtt gcagtgtatg caggaaccag tggagttgct
    10441 cttctaggac aaatgcaaag tgttatttta agtttaaacg ggattataaa ttcacccgta
    10501 ggtaatgggg ttataaaatt cacatcagaa aatagtcatg attttgaaaa atgctcgcca
    10561 tactggcgag catcaatctt atatgttatg tgtttctcta caattataat tatagtcgga
    10621 tgtttttatg cgcaggaaat atccttactt ctttttggac atagtgataa ttactggatc
    10681 gttttattaa tggtatttgt agctcctata acatctatag ggacgctaat taattcaata
    10741 ttgaacggtt tagaaagcta caagcagtat gtttatacag gcattatagc ttctgtactt
    10801 tcaggtctaa taatgatctt tgtgattgtt aatttcggta tagttggcgc gttagttggt
    10861 gcttcacttc aatatgcttt aataggaatt atatctttca ttattaatat atatcagcct
    10921 tggctgaaga ttggctattt aataggtaaa attagcacag atgttaagaa gaatatagca
    10981 gaatatatat ttatggctat agttagtgct gtatcgttac cattaggtat gattttaatt
    11041 agaggctttt taatagaata taccggttgg aaaaatgctg gggagtggca agctgtttgg
    11101 aaaatatctg aggcttattt gagtgtaatt acaatggggc tatcagttta ctatcttcca
    11161 agactcagtt ctttatatga tcataactct ttggttcgtg aagtaaaatc tgtcgcatca
    11221 attgtattac caatagtttc attgatggct gttgcggtat atatcctaag ggatttctca
    11281 atttcattat tgtatactga acagttcaga gaggcaaggg agttatttaa aatccaatta
    11341 ataggtgatg ttataaaagt tattagctgg gtatacgcct atccaatgat agcaaaaaaa
    11401 tcaacaaaac tatttgtatt taccgagatt gtgttcacca taaccttggt tcttttaagt
    11461 tattttttta taaaaaaata tggcgtacat ggagctaatt tagcatatgt actaaattat
    11521 atattatatt tcatatgtgt atatattcta ctgaataaag ctataaaaaa ttaatcttta
    11581 ttattgaagg gggggctgtg ataaccttaa tagttttatc gtataatcat cgcgagttta
    11641 tagtagaaac actaaactct gtatataatg taaatttgca aaaaaaagtg atcattatcg
    11701 atgatgggtc atctgatgga tcatgcgaga ttattgaaaa atatattgaa agatataatt
    11761 atcaatctat cactaggttt ttcacaaagg aaaatgccgg tttagtagac tctttaaata
    11821 tgtcgcttaa gcttattgat acagagtatg cattctttat cgcatcagat gatattatta
    11881 acccttatgg attcacttca atgtttcaga caatgctggc acatgaaaac atgaatatag
    11941 caattggaaa tgcttacgtg ttatatactg gaaaaacacc cgagcaaaaa gtttatggaa
    12001 aagaacattt tgacttcttc tctaaaaaag atagcgagat caggaaggat atttttataa
    12061 atttccccaa accgttgctg ttacaaagca ctatttttaa aacagcttgt ttgagagatg
    12121 ttggaggatg ggattccaag cttgtatggg atgattatcc gatgtttgtt aaattattta
    12181 tgagaaattc ttttgatagg gggaatatat ttttttttgc tgattttgtt gtttctttct
    12241 atcggcagca tcagtttaat acatacaaaa atttattaaa acaattgtct atggttgaag
    12301 aaacatttaa atcaatagtc ccactaaagt attataaaga tgcgatttca aaccagtatg
    12361 ctttctactt tttactatct ttacgcgaaa aaaacaataa agcaataaag aaaataatta
    12421 aagttattgt ttgtgaaaag ttattttttt attttgcaaa gtatttcttc gtacatataa
    12481 ttaagtggat ttctcggaga ttatcaaagt gagggattat ctcttttttg atttcttatt
    12541 cgacaatagc ttggagttct tgatatactt attcactatt tgtatattgt tatttctttt
    12601 atcaaaaaag acagtgggtt atattcttga tcctttccat ttttattaca catttacatt
    12661 tggcacatca tacgcagtta ttttactttt atacatacat gactatattc cagacgatta
    12721 cttttcatat ctattaatca gcaatgttgt tttttttgtg agttatagtc tatcatcaat
    12781 aagaagaagt aagcatgcag gactatttat tagattgggt tattccatcc gatataatga
    12841 aaggtttata cttcagttat tgtggtttat atatttcgct atgctgctga tttacttatc
    12901 aaaggtatca atagctggtc tcctattgtc acgatttgac gcgaataaag gtcttggccc
    12961 aattgtgaga ataatggatg ccttgagact aataattgca gcgtggatgt atataaatta
    13021 cattaggctt gggaaaaaga agtaccttat tattgcatta gttttttctt gtgtttcggc
    13081 atttttttct ggcgctaagt ttgctttgct tgagcaaatg tatgttatgt ttgttgctgg
    13141 ttttgttggg gaaaggataa aaattaaatt acatttgaaa actatagtgc tggctggtgt
    13201 atttttggtt gcgttatttt tatttatttt atttatgttg caaaaattat cagatggaat
    13261 aggctaccag aattcgcaat atattccagg tgcgcctgtt acattagaac tttttatatt
    13321 gcgcatcttg gcgaatgggg attcctacta tttatctctt acccagcggg ttcttgatga
    13381 tataaatatc aatttccctg tgcttcagtt tttttccaat gtttttggaa attcatttat
    13441 gagccgatta tttgggcaag atttctcaaa tggagatatt ggtcgccaaa tatggctata
    13501 ttggtacccg gatgatgata taatgcgtgg tcccactgca catttcgata tatcggggct
    13561 tgtttatttt ggttatatag gtggcgtgat tttttctatt gttataggcg tggttttagg
    13621 ttcgcttaat aaattaaaat attcttgcta tcatgcgccg actgtaattg ttgcttttgt
    13681 atctgcgtta tattgtcgct cattaccttt aatgcttaat ccatctgttg ggttagcgta
    13741 tatcgttgac atatatatgc tgttattttt attattagtt ctttcaacat tatctcgtaa
    13801 aaggattggg taatgagtgt tctagctgtg attgttacgt ataattctga tttgcagaag
    13861 ttgaaaacaa tttttaatat agtatgtatt gatgctgata taatagtgtc tgataattca
    13921 acagaacaga acacacgtga aagaatacaa gaattatgcg agattcattc agttaaatat
    13981 atatcaatgg gcggcaatgt aggtatagca aaagcacaga atgatggtat taaattagga
    14041 ttaaacatgg gctatgagga catattgctt cttgatgatg atagtcttcc tcatccgact
    14101 atgattagtt cgcttttaat agttaggggt tttgttactg aaaatattaa atgtttacct
    14161 gtaatatgtg caaatccttg tgatgcaaaa ggaaactctt tagcaaaata tgataagaaa
    14221 ctttttgatt tcatatataa ttgccgtgat gtcacaagtt cgggcacttt gataaataaa
    14281 agccactttg atttagttgg tttacacgat gaaaaattat ttattgactg tgttgatttt
    14341 gaatggggct ggcgcgcgat atcaaagggc gtgcaaatag tactgtctca aaaggccata
    14401 ttaacacatc aacttggtac tgggaggtta aatataataa atgctggtta tggcgcacct
    14461 atcagacatt attatcaatt taggaatgtg cttaatatgt gcattcgtga atatacccct
    14521 ttcccttgga agatatcgca gattttcaaa cttgggataa aatattttat tcttattcct
    14581 tttttctttg ataataaggt gttaagattg aaatatgcta cgcggggggt tctaagtttt
    14641 attcgcggga aatatggtcc aataaattag attattatct tattagggat actcatgaaa
    14701 attaaagcaa aaataatcta tggttatact attgacttgg ttaatgcttg tgaaaatgat
    14761 gccgaattta ttctttcgtt acgtacaaat atctctaagt caagattttt atcaaaaaca
    14821 agtacggttt tagaggacca aagaaaatgg cttgctacat atagccgcga taacaaacag
    14881 gcctacttta ttataaaaaa caagagaggg gaggatattg ggactgtaag gatatatgat
    14941 ttgcagggat tatcgttttg ttgggggtca tggatactca gcgacaaggc acattctaat
    15001 gcctcgattg agtcggctct tataatatac caatacgcgt tgatgcgtgg atttaaatcc
    15061 gcccatttcg atgtgagaaa agggaataaa tcggtttgca aatttcatga aagatttggg
    15121 gcatttaaag ttggtgaaac taaagacgat tttctttatg acatagatga agataatatt
    15181 atacaatctt tgcatcgtta caagaggttt ttgcctaatt ctattgtttt tgaggaaatg
    15241 taatttcaag ctgtatttat tttagtcatg cgcattcatt atttatatat tcagattatt
    15301 aatctccccc ctgacaggag taaacaatgt caaagcaaca gatcggcgta gtcggtatgg
    15361 cagtgatggg gcgcaacctt gcgctcaaca tcgaaagccg tggttatacc gtctctattt
    15421 tcaaccgctc ccgtgagaag acggaagaag tgattgccga aaatccaggc aagaaactgg
    15481 ttccttacta tacggtgaaa gagtttgttg aatctcttga aacgcctcgt cgcatcctgt
    15541 taatggtgaa agcaggtgca ggcacggatg ctgctattga ttccctcaaa ccatatctcg
    15601 ataaaggtga catcatcatt gatggtggta acaccttctt ccaggacact attcgtcgta
    15661 atcgtgagct ttcagcagag ggctttaact tcatcggtac cggtgtttcc ggcggtgaag
    15721 agggggcgct gaaagggcct tccattatgc ctggtgggca gaaagaagcc tatgaactgg
    15781 ttgctccgat cctgaccaaa atcgccgccg ttgctgaaga tggcgaaccg tgcgttacct
    15841 atattggtgc cgatggcgcg ggtcactatg tgaagatggt tcacaacggt attgaatacg
    15901 gtgatatgca actgattgct gaagcctatt ctctgcttaa aggtggcctg aatctttcca
    15961 acgaagaact ggcgcagacg tttaccgagt ggaataacgg tgaactgagc agctacctga
    16021 tcgacatcac caaagacatc ttcactaaaa aagatgaaga cggtaactac ctggttgatg
    16081 tgatcctgga tgaagcggct aacaaaggta ccggtaaatg gaccagccag agcgcgcttg
    16141 atctcggcga accgctgtcg ctgattaccg agtctgtgtt tgcacgatac atctcttctc
    16201 tgaaagagca gcgcgttgcg gcgtccaaag tactgtctgg tccgcaagca cagccagcag
    16261 gcgacaaggc tgagttcatc gaaaaagttc gccgtgcgct gtatcttggc aaaatcgttt
    16321 cttacgctca gggcttctct cagctgcgtg ctgcgtctga agagtacaac tgggatctga
    16381 actacggcga aatcgcgaag attttccgtg ctggctgcat catccgtgcg cagttcctgc
    16441 agaaaatcac cgatgcatat gccgaaaatc cgcagatcgc taacctgctg ctggccccgt
    16501 acttcaagca aattgccgat gactaccagc aggcgctgcg cgatgtcgtc gcttatgcag
    16561 tacagaacgg tatcccggtt ccgaccttcg ccgctgcggt tgcctattac gatagctacc
    16621 gtgccgctgt tctgcctgcg aacctgatcc aggcacagcg tgactatttc ggggcgcata
    16681 cttataagcg cattgataaa gaaggtgtgt tccatactga atggctggat taatctgatt
    16741 taaccaacaa taaaattgag gcccggcgta tattgcaccg ggcttttttt tgccaattat
    16801 cttatagact aaatttcact gcttaatatt aacttaataa atatcagcta ttcttataaa
    16861 gaaaatctga attgtttttc gttgcgttga ccatcgaaca acgtagcgtt aaaactttta
    16921 gctcttatca ggatgctaaa aacatcatga ttcacagtta agttaattct gagagcatga
    16981 aatgaaaatc accatttccg gtactggcta tgtaggcttg tcaaacgggc ttctaatcgc
    17041 acaaaatcat gaggttgtgg cattagatat tttaccgtca cgcgttgcta tgctgaatga
    17101 tcggatatct cctattgttg ataaggaaat tcagcagttt ttgcaagcag ataaaataca
    17161 ctttaatgcc acattagata aaaatgaagc ctaccgggat gctgattatg tcatcatcgc
    17221 cactccaacc gactatgatc ctaaaactaa ttatttcaat acatccagtg tagaatcagt
    17281 aattaaagac gtagttgaga taaatcctta tgcggttatg gtcgtcaaat caacggttcc
    17341 cgttggtttt accgcagcga tgcataagaa atatcgtact gaaaatatta ttttctctcc
    17401 ggaatttctc cgtgaaggta aagcccttta cgataatctc catccgtcac gtattgtcat
    17461 aggtgagcgt tcagaacgcg cagaacgttt tgctgcgttg ttacaggaag gagcgattaa
    17521 gcaaaatatc ccgaccctgt ttactgactc cactgaagca gaagcgatta aactttttgc
    17581 aaacacctac ctggcgatgc gcgtggcgta ctttaacgaa ctggatagct acgcagaaag
    17641 tttaggtctg aattcccgtc aaataatcga aggcgtttgt ctcgacccac gtattggcaa
    17701 ccattacaac aatccgtcgt ttggttatgg tggttattgc ttgccgaaag ataccaagca
    17761 gttactggcg aactaccagt ctgtgccgaa taacctgatc tcggcaattg tcgatgctaa
    17821 ccgcacgcgt aaagatttta ttaccgatgc cattttgtca cgcaagccgc aagtggtagg
    17881 tatttatcgt ctgattatga aaagcggttc agataacttc cgtgcgtctt ctattcaggg
    17941 gattatgaag cgtatcaagg cgaaaggtgt tgaagtaatc atctacgagc cagtgatgaa
    18001 agaagactca ttcttcaact ctcgcctgga acgtgatctc gccaccttca aacaacaagc
    18061 cgacgtcatt atctccaacc gtatggcaga agagcttaag gatgtggcag ataaggtata
    18121 cacccgcgat ctctttggca gcgactaaca tcctgttatc agggcgattt tcgccctgat
    18181 tctcttatgt tccctttgta ataattcatt atttttatca tttatcctat agcattcatg
    18241 gcgattatcg ctaaactatg gcgacttaaa attatttcgt ccgttagggt aatgatgaga
    18301 gtagaaaata ataatgtttc tgggcaaaac catgacccgg aacagattga tttgattgat
    18361 ttactggtgc agttgtggcg tggcaagatg acaattatca tctctgtcat tgttgctatt
    18421 gccctggcta tagggtatct ggccgttgcc aaagaaaagt ggacttccac agctatcgtt
    18481 actcaacctg atgtggggca aatcgctggt tataccaacg cgatgaatgt gatttacgga
    18541 tcggctgtac cgaaagtctc cgacattcag gcttcgctta tcggacgtta cagcactgca
    18601 ttctcagcat tagcggaaac gctggataat caggaagaac ctgaaaaact gaccattgag
    18661 cctaccgtta aaaatcagtc cttaccttta gcggtttctt atgttggtga aacagctgaa
    18721 ggtgcgcaga aacagctggc gcaatatatc cagcaagttg acgaccaggt aaacgaagaa
    18781 ctggaaaaag acctgaaaga caacatcgcg ctgcgtatga aaaacttgca ggattcgctg
    18841 aagacccagg aagtagtcgc gcaggagcag aaagatctgc gtatccgtca gattcaggaa
    18901 gcgttgcagt atgcgaatca ggcgcaggtg acaaagccac aggttcagca gactgaagat
    18961 gtgacgcaag atacgttatt ccttttgggg agcgaagcgc tggagtcgat gattaagcat
    19021 gaggcgaccc gtccgttggt gttctcacca aactactatc agactcgtca aaacctgctg
    19081 gatatcgaaa gcttaaaagt tgatgatctt gatattcatg cttaccgcta tgtgatgaaa
    19141 ccgacgttac ctattcgtcg cgatagcccg aaaaaggcaa ttaccttgat tctggcggtg
    19201 ctgctgggcg gcatggttgg cgcggggatt gtgctggggc gtaatgctct acgcaattac
    19261 aacgcgaaat aatctttgca gctttaaaga aaacgggcag ggtggtgaca ccttgcccgt
    19321 tttttttgcc ggatacgaca acaacatcgc atccgcttac cccgcaactc actgatgccg
    19381 tttacgcagg ttctcaatca ccgtcgttaa atccagatcc tgatcctgca acagcaccag
    19441 caggtgatac atcaagtcag atgcctcatt cttcagctca aagcggtcat ttaccgtcgc
    19501 ggccagtgcg gtttccaccc cttcttcgcc cactttctgc gcaatgcgtt tagtgccgct
    19561 ggcatacagt ttggctgtgt aagaggtttc cgggtcggca gatttacgtt cggcgagcaa
    19621 ttgttccagt tgatacagga acagccactg gtgagcggtg tcgccgaagc agctgctggt
    19681 gcctttgtgg caggttggcc cgatgggatt cgccagtacc agtaatgtgt cgttgtcgca
    19741 gtccggggca atactcacca cattgagaaa attgcccgac gtttcgcctt tggtccacag
    19801 tcgctgttta gtgcgcgaga agaaggtgac tttgccgctt tcgatagttt tgtctaaagc
    19861 ttccgggttc atatagccca gcattagcac ttcgccggat accgcatgtt gcacaattac
    19921 cggcatcagt ccgtcggttt tttcccagtc cagttcgcga cgttgtagtt ctgttaacat
    19981 atcctgatct ccacgccctg tgttgccagg tacgctttta attcaccaat attgattatt
    20041 tgtttgtgga atacagaagc tgccagcgcg ccgtcaacgt cggcatcgcg gaaggcttca
    20101 aggaagtgct ccatagtgcc cgcgccaccg gaggcaatca gcgggacgtg gcaaacttca
    20161 cgcacttttt tcagttgttc gaggtcgtaa ccgttacgca cgccgtcctg attcatcatg
    20221 ttgaggacga tttctccggc accgcgtttt tgcacttcct gtacccagtc gagcgtttcc
    20281 ca
//
