LOCUS       LC177548               20642 bp    DNA     linear   BCT 15-DEC-2018
DEFINITION  Escherichia coli DNA, O-antigen biosynthesis gene cluster OgN4,
            strain: E2348.
ACCESSION   LC177548
VERSION     LC177548.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., von Mentzer,A., Kikuchi,T. and Thomson,N.R.
  TITLE     An untypeable enterotoxigenic Escherichia coli represents one of
            the dominant types causing human disease
  JOURNAL   Microb Genom 3 (2017) In press
  REMARK    DOI:10.1099/mgen.0.000121
            Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20642)
  AUTHORS   Iguchi,A., von Mentzer,A. and Thomson,N.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2016) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuenkibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..20642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E2348"
                     /db_xref="taxon:562"
                     /note="Escherichia coli O-antigen biosynthesis gene
                     cluster OgN4"
     gene            14..1408
                     /gene="wcaM"
     CDS             14..1408
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAV90437.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGYTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERRQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            1571..2476
                     /gene="galF"
     CDS             1571..2476
                     /gene="galF"
                     /note="OgN4_1"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAV90438.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     CDS             2732..3988
                     /note="OgN4_2"
                     /codon_start=1
                     /transl_table=11
                     /gene="mnnA"
                     /product="UDP-N-acetyl glucosamine 2-epimerase"
                     /protein_id="BAV90439.1"
                     /translation="MQCSGSCKARGGSVFYLNFREHINNHFVRFLIANLHILFKLDTE
                     MSKKIMVVFGTRPEAIKMAPLVKEISNNKALDLRVCVTGQHREMLDQVLEIFQIKPDY
                     DLNLMKSGQSLNDITSSILINIKCILQEYKPDLVLVHGDTATTFAVSLAAYYEKISVG
                     HVEAGLRTGNIYSPWPEEANRKLTAVLAKYHFAPTFSSKENLIREGCVPDNIIVTGNT
                     VIDALHLAKEKINNDIQLSDKLKQEFSFLEPEKKIILVTGHRRESFGKGFEDICDALA
                     IIARSYKDIQIIYPVHMNPNVRGPVNRLLSNINNIYLIEPLQYLPFVYLMDKSYIILT
                     DSGGIQEEAPSLGKPVLVMRDTTERPEAILAGTVALVGTDKNKITEKVKELIENTEVY
                     KKMSAAQNPYGDGKSCQRIVNAILKA"
     CDS             3952..5061
                     /note="OgN4_3"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf4"
                     /product="putative glycosyltranslocase"
                     /protein_id="BAV90440.1"
                     /translation="MPAYRERDIKSIKEEMMRKFYVFESICSEVFHSQFNSAYLSALE
                     GDIVFSSTERHWFYIKKKLKKNIQWKRESQRKKTILSNVLWALKNSKFMAKKAELIFL
                     SSDIFIFYRCIILKLLGFENIKYVTHADLENIKNPRKIKDYVLFFRRHYFLMRLLDAM
                     DIKIVLLSRVIYNNLCEYIKIKNYEIIEHPYLYDEVFTKKKYERENIKIAFLGLPTKE
                     KGFFWLYNELKKNNKIKNCQFYLLGKCENVNYARDRFVFSFEYLDEITDDDIKKELSR
                     MDYVIFPFAEEQYKLRASGTVFDALNARKPILVTNNLFIRDVMGGEEPLGLIFDDNFG
                     IEQLINKLGAIDEKKYEELVSNVDDFVKKRIINAR"
     gene            5051..6439
                     /gene="wzy"
     CDS             5051..6439
                     /gene="wzy"
                     /note="OgN4_4"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAV90441.1"
                     /translation="MPVNKIALISFLSRVLLITLMVIIKIACGYSKILSTVFFIGLLI
                     VSVFLIFLKKKEYTLISLLCVDEINSQNSLLLYLNLAMALCFFVDCLYNRSKTFTNIE
                     LLCVALILGIETSFTIFNIFDKSAISIALQYTINDFKILFVVIVSYFYFKGCDARKIQ
                     VALMFVFISQFLYIIVGYILGLKSTRLVDFDMGVGYSAILFLFLPYIYSKIIFEKNNR
                     FRYLFLTSMLLIAMYKCHILSAQNLLSIPLVTMFVLFTSRRSIVSKMIILSVISITIL
                     LLPLILQLLTSTMDILPDLRGKIENIIAVLSFSGDYYVSSHSVQVRLLEFVNIINEMN
                     PVSLLIGKGLGSSFTDAAYPFIGLDEYDYNLEQIRANEFYTPHNISYFILKFGVGTFI
                     FLTLYSVSKIRKCKPNNLALMVSLFSFSILNMGFSLLPSLALGIFLVMSIDDLKVQQN
                     EFSYKKHNNSVC"
     gene            6399..7643
                     /gene="wzx"
     CDS             6399..7643
                     /gene="wzx"
                     /note="OgN4_5"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAV90442.1"
                     /translation="MNSHIKNTIILFADKLFTLSTGLLIAILAARIFGPAEFGLFNYV
                     TSLIGVLGFIYTLGLENVVINEASNIDNKQKLELLFVNSTALKFLSSLIGIIILLFYC
                     YFFSRESYELALILGFANFILFANSIDFIFQGTYKFNTLVILRLVSKTLMTLVQLCFL
                     LILPNIYYFAAANIVYNSMYVILLWVTANISFSFFMISKEYMLELLKKSLPFTLASVA
                     IPIFMQSDTIMLMHITNNAHETGLYSAVQRILLPASVVGSVLSVSIFSIIKNSVKLQD
                     FSFLIKIHSVIFYGTALLGAIVTYYSEVIMTFLYGNKYLGSGQVLSYSIWIILFSIIG
                     PIGTKMLICANASKVEFYKTAIAAIINIFLNLFFIKKWGAVGACISSLIAYFIANVFI
                     FFCIKRTRFIINIYLKAIFWRY"
     CDS             7643..8710
                     /note="OgN4_6"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90443.1"
                     /translation="MFVFFAKHPGSNPNQGMHHRILAIDNIVRDKERKYVHVSFFKNL
                     RRKITQIDAKTTQYEFNFFIHFFIINKILSNASIIYIHTIINGLKACLLFNKKTVLTI
                     LDAHGVVPEESKMNNEVIKKHVFSLAEKMCFRKVDYIIHVSNAMKEHFDNKYSNRKNR
                     DIILPIFGKMPEHKSINANEHDYILFCYVGGTQKWQNFDDVFAFAINTASPNYRFHFM
                     IPSTDIKKYIEMIPQSIKEYIIINNGGRNEVYDLLQRADFGFLLRDDVLVNNVACPTK
                     AIEYLHFDVIPIVKNNHIGDLNVYGVETLLFSNMECVYMGQLNFEKITKHNKLAYKKM
                     LDDTNKAILELKSIYDERSDY"
     CDS             8688..9878
                     /note="OgN4_7"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90444.1"
                     /translation="MMNVAIINSYFPPVFSGGAENSIDHMLHGLICKPQIKKLIMISL
                     SNIHKEVNVVEKKGIIYYYIPYLYKVCPLSQKRTKLDKLTWYMEDFYSTKSFELVLTI
                     LQEHKIDIVNTNNLAGFSTAILKVIAENNYKIIHTIRDYYFMCIKGNLNHTGCSNTQG
                     GMCKCLTLVRKYHSKFTTAVVGNSKFILDLHLRNGYFKNSKISRHIYNGYSDEQVKYS
                     KALGSTRNKKIGYIGRICKEKGVEIFIQQSMNAAPDNEIIVAGKGDNIYINQLKKMYP
                     SVKFIGHVSPEVFYNQVDFVIVPSLWHEPLARTVFESYFYGLPVIGSNRGGIPESIVN
                     GVNGFIFDPDNPNSLSDSIKLLAYDDEVYEKMSNNAISMSKKFDIETTVNNYVALYQE
                     VING"
     CDS             9871..10818
                     /note="OgN4_8"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90445.1"
                     /translation="MARVIRISASDAKYGGNIYESNINKALQNFNITTFFALSWVPKK
                     VRYFFAPIFYLLNFIKCNFCDADIVIVPLEAISFLSKKKKNIVVVHHIDHSFSSFFSS
                     LNQIFTYFLLVLNKNRINKIVVVSEYWEKYLQGKGFNNIQIIYNDIDISLRDEVKKSI
                     RNFRVEGKTNIYIGNFHKKKGTYDLLSLLKDHYVLHTSGYKGQQIEGLINHSRLSYPD
                     YLKLLSDMDVCITYSRFREGWCRTAHEALILGTKVIGSGYGGMGELLKGSSQPIASDY
                     LSLKIALENYDLFEIDSDYVTSFDFERFKKSWSELVEKL"
     CDS             10791..11582
                     /note="OgN4_9"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90446.1"
                     /translation="MVRIGGKIMNFINIEQPLVSIIMACYNSIKTIDESISSVINQDY
                     KNFELIIADDNSTDGTYEFIISKYSSSDKVKIIRNEYNKGVSGARNSALRKAKGRYIC
                     FLDSDDLWETTKLSTQITYMLKHNVTLSYSDYTVFVIKDGHTKRKKLKFCEKVSYKEM
                     LKTCSFGCLTVCYDVNYFGKVYFDDVVKEDYVCWLSLLKRVPYAYNVGVDIARYRQQK
                     QSLSSNKIKEIKKQFYVISKIEGNNSILSIYNLLFYIFNGLIKRV"
     gene            11582..12979
                     /gene="manC"
     CDS             11582..12979
                     /gene="manC"
                     /note="OgN4_10"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="BAV90447.1"
                     /translation="MRNIIPVIMAGGSGTRLWPLSRTEYPKQFLALINKHSMLQNTIN
                     RIKELQHSAPIIICNNEHRFLVSEQLKQIGSNTNGIILEPCGRNTAPAIALAALRAME
                     LEEDPVLLVLAADHSIDDEDIFINSILHAFPHALNNKLVTFGVTPSKPETGYGYIKIG
                     ETLINNAYLIESFKEKPNVELAEYYLKSGDYLWNSGIFLFKASKFLDELKKLSPSIYD
                     ACEKSYKDAQIDLDFIRVDKDKFELCPSDSIDYAVMEKTRAGVVIPLNASWSDVGSWT
                     SLWDISEKDENGNHIIGDSLLIDSYNSYIRADNTLIAAIGLRNLVIVQTKDAILIAEK
                     ERVQDVKFIVDKLKKEQREEYKIHRESYKLWGRSECIDKNSGYLVNKITINPGNKIKM
                     QFHLRRTEHWIILAGTAKVTSNNQVQFLTENQSIYIPIGVSHEIENLGKIPLVFLEIQ
                     SGEYLSEDDIIRVDS"
     gene            12993..14426
                     /gene="manB"
     CDS             12993..14426
                     /gene="manB"
                     /note="OgN4_11"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="BAV90448.1"
                     /translation="MKSKYISKNIIALSKVQFGTSGARGLIADFSNDVCVAFTHSFLT
                     VVEQKFSFTSVALAIDNRPSSYEIAQACAFAIKQHGFTVEYHGVIPTPALAHYSMQKN
                     IPCIMVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFSPVDVLPHLETSTQGA
                     DYYLERYVSLFNPEILKGKRIGVYEHSSAGRDLYAPLFNQMGAEVISLGRSDEFVPID
                     TEAVSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELN
                     IKALAIPVSCNTAIEESNKFASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFLL
                     ASDLQINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFPSD
                     SSQQIIKNAISSPNNFFNSLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPELR
                     CYAEASDENQAKQYVTNVLGNITSLIS"
     gene            14623..16029
                     /gene="gnd"
     CDS             14623..16029
                     /gene="gnd"
                     /note="OgN4_12"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAV90449.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            16251..17444
                     /gene="ugd"
     CDS             16251..17444
                     /gene="ugd"
                     /note="OgN4_13"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAV90450.1"
                     /translation="MFNRKKSELMKITISGTGYVGLSNGILIAQNHEVVALDIVQAKV
                     DMLNKKQSPIVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFN
                     TSSVEAVIRDVTEINPDAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYD
                     NLHPSRIVIGEQSDRAERFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVA
                     YFNELDSYAESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQS
                     VPNNLISAIVDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRI
                     KAKGVPVIVYEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRD
                     LFGND"
     CDS             complement(17510..18514)
                     /note="OgN4_14"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf15"
                     /product="NAD-dependent epimerase"
                     /protein_id="BAV90451.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYSLPTTGLRFFTVYGPWGRPDMALFKFTKAILEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKNPQWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            19157..19900
                     /gene="wzz"
     CDS             19157..19900
                     /gene="wzz"
                     /note="OgN4_15"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAV90452.1"
                     /translation="MIYGSAAPKVSEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIE
                     PTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVNEELEQDLKDNIALQMKNLQD
                     SLKTQEVVAQEQKDLRICQIQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALES
                     MIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAI
                     TLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(19996..20607)
                     /gene="hisI"
     CDS             complement(19996..20607)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAV90453.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLVNPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSANPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     6524 a   3569 c   4205 g   6344 t
ORIGIN
        1 tagaggagaa cgcatgccat ttaaaaaact ctcccgacgc accttcctga cggcaagctc
       61 ggcgcttgcc ttcctccata cccctttcgc tcgcgcactt cccgcccgac aaagcgttaa
      121 cattaacgac tacaacccac acgactggat cgcctcattt aaacaagcct tcagcgaagg
      181 gtatacggtc gtcgtgcctg ccggattggt ttgtgacaat atcaacacag gtatctttat
      241 ccctcccggt aaaacgttac acatccttgg aagcctgcgc ggcaacggca gagggcgatt
      301 tgtcttacag gacggcagcc aggtgacagg ggaggagggc ggcagtatgc ataacatcac
      361 cctggatgtg cgcggctctg actgcaccat caaagggctg gcgatgagcg gctttggccc
      421 ggtaacgcag atttatattg gcggcaaaaa caaacgggtc atgcgtaacc tgaccatcga
      481 taacctgacc attagccacg ctaattacgc catcttacgg cagggatttc ataaccagat
      541 tatcggtgcc aacatcacca attgtaagtt cagcgactta cagggggacg ctattgaatg
      601 gaacgtggcg attaacgaca gtgatatttt gatatctgac catgttatcg agcgcatcaa
      661 ctgtaccaac ggcaaaatca actggggcat cggcataggt cttgcgggaa gtacctacga
      721 taacaactac ccggaagacc aggcagtgaa aaactttgtc gtggcgaata tcacgggatc
      781 ggattgtcgg cagttgatcc atgttgaaaa tggtaaacat tttgttattc gtaatatcaa
      841 agcccgcaat atcacgccgg atttcagtaa gaaagcaggt attgataacg ccacagtcgc
      901 tatttacggt tgtgacaatt tcgtgattga taatattgaa atgattaata gcgccgggat
      961 gttaatcggc tatggggtaa ttaaaggcaa atatctctcg ataccgcaaa atttccgagt
     1021 gaataatatt caactggata acacccatct tgcttataaa ttgcgcggta tccaaatctc
     1081 cgccgggaat gctgtctcct ttgtggcgct gactaacatt gagatgaagc gtgcgtcgct
     1141 ggagttacac aacaaaccgc aacatctttt tatgcgtaat atcaaagtga tgcaggaatc
     1201 ctcagttgga ccagcattga gcatgaactt cgacatgcgc aaagacgttc gcggcgtctt
     1261 tatggcgaaa aaagaaaccc tgctatctct tgcaaatgtt catgcggtga atgaaagaag
     1321 gcaaagctcc gtcgatatcg acaggattaa tcaccatatt gttaatgtgg aaaagattaa
     1381 ctttagattg ccggaacgga gggagtagat ttgcgaccat tcctggaaaa atggagccat
     1441 acttaggaac aatgctactg caatccacaa cgaagcggcg taacatcaca agtaattcag
     1501 taatcaattc agggtaattg atgctggcga aaaaaatcga acaagctata attcagcaac
     1561 cattttacag gtggatgaaa taatgacgaa tttaaaagca gttattcctg tagcgggtct
     1621 tgggatgcat atgttgcctg ccactaaggc gattcccaaa gagatgctac cgatcgtcga
     1681 caagccaatg attcagtaca ttgttgacga gattgtggct gcagggatca aagaaatcct
     1741 cctggtaact cacgcgtcca agaacgcggt cgaaaaccac ttcgacacct cttatgaatt
     1801 agaatctctc cttgagcagc gcgtgaagcg tcaactgctg gcggaagtac aatctatctg
     1861 tccgccgggc gtgaccatta tgaacgtgcg tcagggtgaa cctttaggtt taggccactc
     1921 cattttatgt gcgcggcctg ccattggtga caatccattt gtcgtggtgc tgccagacgt
     1981 tgtgatcgac gacgccagcg ccgacccgct gcgctacaac cttgctgcca tgattgcacg
     2041 attcaacgaa acgggtcgta gccaggtgct ggcaaaacgt atgccgggtg acctctctga
     2101 atactcggtc atccagacca aagaaccact ggaccgtgaa ggtaaagtca gccgcattgt
     2161 tgaatttatc gaaaaaccgg atcagccgca gacgttggac tcagacatca tggccgttgg
     2221 tcgctatgtg ctttctgccg atatttggcc ggaacttgaa cgcacgcagc caggtgcatg
     2281 ggggcgtatt cagctgactg atgccatcgc tgaactggcg aaaaaacagt ccgttgatgc
     2341 tatgctgatg acaggtgaca gctacgactg cggtaaaaaa atgggttata tgcaggcatt
     2401 tgtgaagtat ggactacgca acctgaaaga aggggcgaag ttccgtaaag ggattgagaa
     2461 gctgttaagc gaataatgaa aatctgaccg gatgtaacgg ttgataagaa aattataacg
     2521 gcagtgaaga ttcgtggcga aagtaatttg ttgcgaattt tcctgccgtt gttttatata
     2581 aacaatcaga ataacaacga gttagcaata ggattttagt caaagttttc caggattttc
     2641 cttgtttcca gagcggattg gtaagacaat tagcgtttga atttttcgga tttggcgcga
     2701 gtgggtaacg ctcgctacat cgtaggcatg catgcagtgc tctggtagct gtaaagccag
     2761 gggcggtagc gtgttttatt taaactttcg agagcatata aataatcact ttgtcagatt
     2821 tttaatagct aatttacata ttttatttaa attggatact gaaatgtcta aaaaaataat
     2881 ggttgtattt ggaacgagac ctgaagcaat taaaatggct cctctcgtca aagaaataag
     2941 caataataaa gctttagatc ttcgcgtatg tgtaacaggg cagcatcgcg aaatgctaga
     3001 ccaagtacta gaaatatttc aaataaaacc tgactatgac ttaaatttga tgaagtctgg
     3061 gcagtctctt aatgatatta cgtcatcaat attgattaat attaagtgta tcttacaaga
     3121 atataagcca gaccttgtgc tggttcatgg tgataccgca actacatttg ccgtgagttt
     3181 agctgcttac tatgagaaaa tttcagtggg gcatgtggaa gcaggtttac gaactgggaa
     3241 tatttattca ccttggccag aggaagcaaa tagaaaacta acagcggtat tggcaaagta
     3301 tcattttgct ccaacttttt cttcaaaaga aaatttaata agagaagggt gcgtacctga
     3361 caatataata gttactggaa ataccgttat agatgcatta catttggcta aagaaaaaat
     3421 aaataacgat attcaattat cagataaact caaacaggaa ttttcttttt tagaaccaga
     3481 aaagaaaata atacttgtaa ctggacatcg acgagagagt tttggaaaag gattcgagga
     3541 tatctgcgat gctctagcaa ttattgctcg tagttataag gatatacaga ttatctatcc
     3601 agtgcatatg aatcctaatg ttcgaggtcc tgtgaacagg ttgttgtcaa atataaataa
     3661 catatattta attgaaccac tgcagtattt accttttgtt tatttaatgg ataaatctta
     3721 tattatatta acagattctg gtggcattca ggaggaagcc ccctcattag gtaaacctgt
     3781 tttagtgatg cgagatacaa cagaaagacc agaagcgatc ttggccggca ctgttgcttt
     3841 agtaggtaca gataaaaata aaattactga aaaagttaag gagttaatag aaaacactga
     3901 ggtgtataaa aaaatgagtg cagcacaaaa tccttatggt gatggtaaat cttgccagcg
     3961 tatcgtgaac gcgatattaa aagcataaag gaggagatga tgcgtaaatt ttatgtgttt
     4021 gagtctattt gttcagaggt atttcatagc caatttaatt cagcctatct ttcagcatta
     4081 gaaggcgata ttgtattcag tagtactgaa cgtcattggt tttatataaa gaaaaaatta
     4141 aaaaaaaaca ttcaatggaa aagagaaagt cagcgcaaga aaacaatttt atcaaatgtt
     4201 ttatgggcgt taaaaaactc taagtttatg gcgaaaaagg cagagttaat atttttgtcg
     4261 tcagatattt ttatttttta tagatgtatt atattaaaac tacttggttt tgaaaacata
     4321 aaatatgtta ctcatgccga tttggaaaac ataaaaaatc ctagaaaaat aaaagattat
     4381 gtactatttt ttagaaggca ttatttttta atgagattat tggatgcaat ggatattaaa
     4441 atcgtgttat tgtcgcgtgt aatatacaat aatctatgcg aatatataaa aataaagaat
     4501 tatgaaatca ttgagcatcc atatttatat gatgaggtat ttaccaaaaa gaaatatgag
     4561 agagaaaaca taaaaattgc ttttttaggg ttaccaacaa aagagaaagg ttttttttgg
     4621 ttgtataatg agttaaaaaa gaataataaa ataaaaaatt gtcagtttta tcttctggga
     4681 aagtgcgaaa atgtgaatta cgcgagggat cgttttgtgt tcagtttcga atatttagat
     4741 gaaataacgg atgatgatat caaaaaagaa ttaagtagaa tggattacgt tatatttcca
     4801 tttgcggaag aacaatacaa attaagagca agtggtactg tgtttgatgc tttaaatgcg
     4861 agaaagccaa ttctagttac aaataatcta tttataagag atgtaatggg aggtgaggaa
     4921 ccattggggc taatatttga tgataacttt ggtatagaac aattaataaa caaactcggc
     4981 gctattgatg aaaaaaaata cgaagagctt gtaagtaatg tggatgattt tgttaaaaag
     5041 agaattataa atgcccgtta ataaaattgc tcttatatca tttttatcga gagtgttatt
     5101 aattacttta atggtaataa ttaaaattgc ttgtggatat agcaaaatac tatctacagt
     5161 attctttata ggactgttga ttgtttctgt attcttgatc ttcttgaaaa agaaagaata
     5221 cactttgatt agtctcttat gtgtggatga aataaactca caaaatagct tgctgctcta
     5281 tttgaatctt gcaatggcgc tatgcttctt cgtagattgc ctttataata ggagcaaaac
     5341 atttacgaat attgagttgc tttgtgttgc tttaatatta ggaatagaaa catcatttac
     5401 tatatttaat atattcgata aatcagcgat atcaattgca ttgcaatata caataaatga
     5461 ttttaagata ctgtttgtcg tcattgtgtc ttatttttat tttaagggct gtgatgctcg
     5521 aaaaatacaa gtggcattaa tgtttgtttt tatttcgcaa tttttataca ttattgtggg
     5581 atatatattg gggttgaaat caacaagact tgttgatttt gatatggggg tgggttacag
     5641 tgctatccta tttctatttt taccatatat ttattcaaaa ataatatttg aaaaaaacaa
     5701 taggtttcga tatttgtttt taaccagcat gttgctaata gcaatgtata aatgtcacat
     5761 actgagtgcg caaaatttgc tttcaatacc gttagtcaca atgtttgtat tatttaccag
     5821 cagacgtagt atagttagta aaatgattat tctgagtgtt atatcaataa ctatattatt
     5881 gctgccactt atacttcagt tgttaacgtc tactatggat atacttcctg acttaagagg
     5941 taaaattgag aatattattg ctgtcttaag ttttagtggt gattattatg taagtagtca
     6001 ttcagttcag gttcgattgt tagagtttgt taatataatt aatgagatga atcctgtttc
     6061 tctattaata ggaaaaggac tgggaagtag ttttactgat gcggcctatc catttatagg
     6121 attggatgaa tatgattata atttagaaca aataagagca aatgagtttt acacaccaca
     6181 taatatttcc tattttatac tcaaatttgg ggtcggtacc ttcatttttc tgacacttta
     6241 ttctgtctca aagatcagaa agtgcaagcc aaataatttg gctttgatgg tttcattatt
     6301 tagtttttct attcttaata tggggttttc attgctccca tctcttgcat taggtatatt
     6361 tctcgtaatg agtatagatg atcttaaggt tcaacagaat gaattctcat ataaaaaaca
     6421 caataattct gtttgctgat aagttattca cattatctac aggattatta atagcaatat
     6481 tggctgctag aattttcggt cctgctgaat ttggtctttt taattatgtg acaagcttga
     6541 ttggggtgtt aggctttatt tatactctag ggttggagaa tgttgtaata aatgaagcaa
     6601 gcaatataga taataaacaa aagctggagc tgctttttgt caattcaaca gcacttaaat
     6661 ttttatcctc attgatagga ataattatac ttctatttta ctgttacttc ttttctagag
     6721 aaagttatga attagcatta attttggggt ttgcaaactt catattgttt gcaaattcaa
     6781 ttgattttat attccaaggg acatataagt ttaatacttt ggtcatactg cgattagtat
     6841 cgaaaacact aatgacatta gttcaattat gttttttgct tattttgccc aatatatatt
     6901 attttgcagc agcaaatatt gtatataatt caatgtatgt aatattgcta tgggtaacgg
     6961 cgaatatatc gtttagtttc ttcatgataa gtaaagaata tatgctggag cttttgaaaa
     7021 aaagcttgcc atttacattg gcttcagtag caatacctat atttatgcaa agtgatacaa
     7081 ttatgctcat gcatataaca aataatgcac atgagactgg tttgtatagt gccgttcaga
     7141 gaatattatt acctgcaagt gttgtcggtt ctgtcttatc tgtatctatc ttttcgataa
     7201 ttaaaaatag tgttaaatta caagattttt catttttaat caaaattcac tcagttatct
     7261 tttatggtac tgctttactt ggggcaattg ttacttatta ctctgaagta ataatgacat
     7321 ttctatacgg caataagtat ttaggttctg gacaggttct ttcatattct atatggataa
     7381 ttttattttc tattatagga ccaattggca caaaaatgtt aatatgtgca aatgcatcaa
     7441 aggtcgagtt ctataaaaca gcaatagcgg ctattataaa cattttcctt aatttgtttt
     7501 tcataaaaaa atggggagct gtcggtgctt gtatttcatc attaattgca tactttattg
     7561 caaatgtttt tatatttttt tgcatcaaaa ggactcgttt tattattaac atctatctaa
     7621 aagcaatatt ctggagatat taatgtttgt tttttttgcg aaacatccag gtagcaatcc
     7681 taatcaaggc atgcatcatc gaatactggc gatagataat attgtaagag ataaagagag
     7741 gaagtatgta catgtttctt tctttaaaaa tttacgaaga aaaattacac aaatagatgc
     7801 taaaaccact caatatgaat ttaatttttt tatacatttt tttattataa ataaaattct
     7861 aagcaatgcc tcaattatat atattcatac catcattaat ggactaaaag cttgtctttt
     7921 attcaataaa aaaacagtat taaccatact ggatgcacat ggtgtagtac ctgaagaaag
     7981 taaaatgaat aatgaagtaa taaaaaagca tgtattctcg ctcgcggaaa aaatgtgctt
     8041 taggaaagtt gattatataa ttcatgtctc caatgcaatg aaagaacatt ttgacaataa
     8101 atattcaaat aggaaaaatc gagatataat attaccaatc tttggtaaaa tgcctgaaca
     8161 taaatcaatt aatgcaaatg aacatgacta tatattgttt tgttatgtag gaggaactca
     8221 aaaatggcaa aactttgatg atgtttttgc ttttgcaatc aatacggcat ctcctaatta
     8281 ccgattccat tttatgattc cctcaactga tataaaaaaa tatattgaga tgattcctca
     8341 aagcataaaa gaatatatta tcataaataa tggtggaagg aatgaagtat atgatttatt
     8401 acaaagggct gatttcggtt ttttgctaag agatgatgtt ttagtgaata atgtggcttg
     8461 tccaacaaaa gcaatagaat atcttcattt tgatgtaatt ccaattgtga aaaataatca
     8521 cattggtgat ttgaatgtat acggcgtaga aacattactt tttagcaata tggaatgcgt
     8581 atatatgggg caattaaatt ttgaaaaaat cacaaaacat aacaaattag cttataaaaa
     8641 gatgctggat gacacaaata aagcaatttt agaattgaag agtatttatg atgaacgtag
     8701 cgattattaa ttcatatttc cctcctgttt ttagtggtgg ggcagaaaat tcaatagacc
     8761 atatgctcca tggtttaata tgtaagcctc aaattaaaaa actgataatg atatcattat
     8821 caaatattca taaagaagta aatgttgtag aaaaaaaagg gattatttac tactatatac
     8881 catacttata taaagtttgc ccattatctc aaaaaagaac taaacttgat aagttaactt
     8941 ggtatatgga agatttttac tcaaccaaat catttgaact agtattgaca atattacaag
     9001 aacataaaat tgatattgtt aatacaaata atttagccgg ctttagtact gcgatattaa
     9061 aagtaatagc ggaaaataat tataaaatca ttcatacaat tcgtgattat tatttcatgt
     9121 gcattaaggg aaatttaaat cacacgggat gtagcaatac tcaaggggga atgtgtaaat
     9181 gtcttacgct tgtaagaaaa tatcattcta aattcacaac tgcagttgta ggtaatagta
     9241 aatttatatt agatttgcac ctacggaatg gttattttaa aaattcaaaa atatcgagac
     9301 atatatataa cggttactct gatgaacaag taaaatatag taaagcgctt ggtagtacaa
     9361 gaaataaaaa aattggctat attggtcgta tctgtaaaga aaaaggtgta gaaatcttca
     9421 tacaacaatc aatgaatgcg gcacctgata atgaaataat tgttgccggc aaaggtgata
     9481 atatttatat aaatcaactt aaaaaaatgt acccctcagt taaatttatt ggacatgtat
     9541 ctcctgaagt attttataac caagttgatt ttgtaattgt tccatcatta tggcatgagc
     9601 ctttagcgag aacagttttt gaatcgtatt tttacggact acctgttata ggctcaaatc
     9661 gtggtggtat acctgagtcc attgtaaatg gagtaaatgg atttatcttc gatcctgata
     9721 atcccaattc attatcagac agcataaaat tgttggcata tgatgatgaa gtctatgaaa
     9781 aaatgagcaa caatgctata tcgatgtcaa aaaaattcga tattgaaaca acggtcaata
     9841 attatgttgc tttatatcag gaggtcataa atggctagag ttataagaat tagtgccagt
     9901 gatgcaaagt atggtggtaa tatttatgag agtaatatca ataaggcatt gcagaatttt
     9961 aacatcacta cattttttgc actatcatgg gtaccaaaga aagtaaggta tttttttgca
    10021 ccgatattct atttgttaaa ttttattaaa tgtaattttt gtgatgctga cattgttata
    10081 gttcctttag aagccataag ttttttatca aaaaagaaaa aaaacattgt tgttgttcat
    10141 catatcgatc actctttttc ttctttcttc tcaagtctta atcaaatctt tacctatttc
    10201 ctattagttt taaataagaa tagaattaat aagattgtag tggttagtga atattgggaa
    10261 aaatatttac agggaaaagg gtttaataac attcagatta tatataatga catcgatatt
    10321 tcattaagag atgaagtcaa aaaatctatt agaaacttta gagttgaagg taagacaaat
    10381 atatacatcg gcaattttca taaaaaaaag ggaacctatg atttgttatc cttgctaaaa
    10441 gatcactatg ttcttcatac aagtgggtac aaaggtcagc aaatcgaagg attgataaat
    10501 cacagtaggt taagttatcc cgattatctg aaattattat ctgatatgga tgtatgtata
    10561 acctactcgc gttttcgaga aggatggtgt cgcacagccc atgaggcgtt aatacttggt
    10621 actaaggtca ttggctctgg ttatggaggg atgggggagc ttttgaaagg ctcttcacag
    10681 cctatagcaa gtgattacct gtcattgaaa atagctttag aaaattatga tttatttgaa
    10741 attgatagtg attatgttac cagttttgat tttgaaaggt ttaaaaaatc atggtcagaa
    10801 ttggtggaaa aattatgaac ttcatcaata ttgaacagcc gttagtttcg ataattatgg
    10861 cgtgctataa ttccataaaa acaatagatg aatctatttc atctgttatt aatcaagatt
    10921 ataaaaactt tgagcttatt attgctgatg ataattctac agatgggaca tatgaattta
    10981 taatcagtaa atattccagt tctgataaag ttaaaataat tcgtaatgaa tacaataaag
    11041 gtgtttctgg agctagaaat tcagctttaa gaaaggctaa aggaagatat atatgctttt
    11101 tggattctga tgatttatgg gaaacaacaa aattatcaac ccaaattact tatatgctta
    11161 agcataatgt tactctatca tattctgatt acacagtatt tgtaataaaa gatggtcata
    11221 ccaagaggaa aaaactgaag ttttgtgaga aggtaagtta taaggaaatg ctaaaaacct
    11281 gtagtttcgg gtgtttaact gtttgctatg atgtaaatta ttttggaaaa gtttattttg
    11341 atgatgtcgt aaaagaagac tatgtttgct ggttgagctt attaaaaaga gttccttatg
    11401 cttataatgt tggcgtagat atcgcgagat atcgacaaca gaaacagtca ctttcttcta
    11461 ataagatcaa agaaataaaa aaacaatttt atgtcatttc caaaatagag ggaaataatt
    11521 caattttatc aatctacaat cttctttttt atatttttaa tgggctaatt aagagagtgt
    11581 aatgagaaat ataatacccg taataatggc tggtggttct ggtacaagac tttggccgct
    11641 atctcgaaca gaatatccta aacagttttt ggcattaata aacaagcatt caatgctcca
    11701 aaacacaata aatcgtatta aagaattaca acatagtgct ccaataataa tttgtaataa
    11761 tgaacatagg tttttagttt ctgaacaatt aaaacaaatt ggttcaaata ctaacggtat
    11821 catactagaa ccttgtggaa ggaatactgc accagcaata gcattagccg cattaagagc
    11881 aatggagctc gaagaagatc ctgttttgct ggttttagct gcggatcatt caattgatga
    11941 tgaagatatt tttataaatt ctattcttca tgcttttcct catgcattaa ataataagtt
    12001 agttacattt ggagtgactc catcaaaacc agaaacaggt tacggatata tcaaaattgg
    12061 cgaaactctt ataaataacg cttatttaat tgaaagtttt aaagaaaaac caaatgttga
    12121 acttgctgaa tattatttaa aaagtggaga ttatctttgg aatagtggta tctttctatt
    12181 taaagcatct aaattccttg atgagctaaa gaaattatca ccatctatct atgacgcatg
    12241 tgaaaaatca tacaaagatg cacaaattga tcttgatttt attcgagtcg ataaagacaa
    12301 atttgaatta tgcccaagcg attcaataga ctatgcggta atggaaaaaa cacgagcagg
    12361 ggtggttata ccattaaatg cttcatggag tgatgtcggg tcatggacat cattatggga
    12421 tatttcggaa aaagatgaaa atggaaacca tattattggt gatagtttac tcatagattc
    12481 atataatagc tatatccgag ctgataatac acttattgct gcaataggtc ttagaaattt
    12541 ggttatagtc caaactaaag atgcaattct tatcgcagaa aaagaacgag tacaagatgt
    12601 aaagtttatt gttgataagt taaaaaaaga acaaagagaa gaatataaga tacatcgaga
    12661 aagttataaa ctatggggac gctcagaatg tattgataaa aattctggct atcttgttaa
    12721 taagatcact attaatcccg ggaataaaat taagatgcaa ttccacctga ggcgtactga
    12781 acattggatt atacttgccg ggacagcaaa agtaacatca aataatcaag ttcaattcct
    12841 cactgaaaat caatcaattt atattccgat aggagtttca catgaaatag aaaatcttgg
    12901 taaaattcct ttggtatttt tagagataca atcaggtgaa tatctttctg aagacgatat
    12961 tatacgtgtt gatagttaac ggttaagaaa atatgaaatc aaaatatatc tcaaaaaata
    13021 ttattgcttt atcaaaagtt caatttggta ctagtggtgc tcgtggctta atagcagatt
    13081 ttagtaacga tgtatgtgtc gcctttaccc attcttttct taccgttgta gaacaaaaat
    13141 tttcttttac tagtgttgca ttagcaattg acaatcgccc tagtagttat gagatcgctc
    13201 aggcatgcgc attcgctatc aaacaacatg ggttcactgt tgaatatcat ggtgtaattc
    13261 cgactcctgc attagctcat tattcgatgc agaaaaacat tccctgcata atggtcacag
    13321 ggagtcatat cccttttgat cgtaatggct taaaattcta cagacctgat ggtgaaatca
    13381 ctaaagagga tgagctcgca attataaata gtgagtatac attttcgcct gtagatgtat
    13441 tacctcatct tgaaacaagc actcaaggtg cagactacta ccttgagcgt tatgtttctc
    13501 tttttaatcc cgagatttta aaggggaaaa gaataggggt atatgaacat tctagtgcgg
    13561 ggcgcgattt atatgctcct ctttttaatc aaatgggtgc agaggtcatt tccctcggta
    13621 ggagtgatga gttcgttccg attgatacgg aagcagtaag cgatgaagat cgtatacttg
    13681 caagagagtg gtctaaaaaa tataatcttg atgctatttt ctcaacagat ggcgatggtg
    13741 atcgtccttt agttgctgat gaaaatggtg aatggttaag aggcgatatt ctgggactac
    13801 ttactgctat tgaacttaat atcaaggcgt tggctattcc agtgagttgt aatacagcaa
    13861 ttgaagagtc taataaattt gcaagtatac aacgaacgaa aataggttct ccttatgtaa
    13921 ttgcagcatt tgcagatctt gctaagcaat ttgattcagt cgcaggtttt gaagctaatg
    13981 gtggttttct ccttgcctcc gatttacaaa ttaatggcaa ggaattaaaa tcgttgccta
    14041 cacgagatgc tgtgttacca gcattaatgc tcttaatagc ttctcgcaat agtactattt
    14101 ctcaactgat taataatctt cctcagcgat tcacttggtc agatagagtt aaaaacttcc
    14161 cttcagattc aagtcaacaa attataaaaa atgccatatc gtcacccaat aatttcttta
    14221 atagtttagg ttatgaatca ttatcctgtt ccgctattga tgaaacggat ggtgcaagat
    14281 ttactttaaa taatggtgat attatacatc tccgtccttc cggtaatgct ccagaactcc
    14341 gttgttatgc tgaggccagt gatgaaaatc aagctaagca atatgttacg aatgtgctgg
    14401 gaaatattac ctctttgatt tcttgatgtt ataggtttat ctacgcttat atgtgtgcgt
    14461 aggtttgatt acacgtagat gccggtatac agaattgaag aacggtattt gttgcattaa
    14521 tgaaattcag cactacacac attcgtgcaa cttgagataa catctcaatc atattcaagt
    14581 cgcgcaaaca tcgcggtgaa caccccctga caggagtaaa caatgtcaaa gcaacagatc
    14641 ggcgtcgtcg gtatggcagt gatggggcgc aaccttgccc tcaacatcga aagccgtggt
    14701 tataccgtct ctattttcaa ccgttcccgt gagaaaacgg aagaagtgat tgccgaaaat
    14761 ccgggcaaga aactggttcc ttactatacg gtgaaagaat ttgttgaatc tctggaaacg
    14821 cctcgtcgca tcctgttaat ggtgaaagca ggtgcaggca cggatgctgc tattgattcc
    14881 ctcaaaccat atctcgataa aggtgacatc atcattgatg gtggtaacac cttcttccag
    14941 gacaccattc gtcgtaaccg tgagctttct gcagaaggct ttaatttcat tggtaccggt
    15001 gtttccggag gtgaagaagg tgcgctgaaa ggaccttcca tcatgcctgg tgggcagaaa
    15061 gaagcctatg aactggttgc accaatcctg accaaaatcg ccgcagtagc tgaagacggg
    15121 gagccatgcg ttacctatat tggtgccgat ggcgcaggtc actatgtgaa gatggttcac
    15181 aacggtattg aatacggtga tatgcagctg attgctgaag cctattctct gcttaaaggt
    15241 ggcctgaatc tttccaacga agaactggcg cagacgttta ccgagtggaa taacggtgaa
    15301 ctgagcagct acctgatcga catcaccaaa gatatcttca ccaaaaaaga tgaagacggt
    15361 aactacctgg ttgatgtgat tctggatgaa gcagcaaaca aaggtacggg aaaatggacc
    15421 agccagagcg cgctggatct cggcgaaccg ctgtcgctga ttactgagtc tgtgtttgca
    15481 cgttatatct cttctctgaa agatcagcgt gttgccgcat ctaaagttct ctctggcccg
    15541 caagcacagc cagcaggcga taaggctgag tttatcgaga aagttcgtcg tgcgctgtat
    15601 ctgggcaaaa tcgtttctta cgctcagggc ttctctcagt tgcgtgctgc gtctgaagaa
    15661 tacaactggg atctgaacta cggcgaaatc gcgaagattt tccgtgctgg ctgcatcatt
    15721 cgtgcgcagt tcctgcagaa aatcaccgat gcatatgccg aaaatccgca gatcgctaac
    15781 ctgctgctgg ctccgtactt taagcaaatc gccgatgact atcagcaggc gctgcgtgat
    15841 gtcgttgctt atgcagtaca gaacggtatc ccggttccga ccttcgccgc tgcggttgcc
    15901 tattatgaca gctaccgcgc cgctgttttg cctgcgaatc tgatccaggc acagcgtgac
    15961 tatttcggtg cgcatactta taagcgcatt gataaagaag gtgtgttcca taccgaatgg
    16021 ctggattaat ctgatttaaa tcaattaatc aatgcaaggc ccggagaaac cctccgggct
    16081 tttttattat acaaagcggc aggttatggc ctttttttat aatttatggt taaaaaagcg
    16141 atataataca gagccgcaca gcaggatcgc tgccttgaca gttcaaccgc atcagcgtta
    16201 aaaatcccgc agtagatgaa gctgtggtgg tggattaatg accactctaa atgtttaacc
    16261 ggaagaagtc agagctaatg aaaataacaa tttcaggaac aggttatgtt ggtctttcaa
    16321 atggtattct gattgcgcaa aaccacgaag tggttgcact ggatatcgtt caggccaaag
    16381 tggacatgct taacaagaag cagtcaccga ttgttgataa ggagattgaa gagtatctgg
    16441 cgactaaaga tctcaatttc cgcgctacga cagataagta tgacgcgtat aaaaatgccg
    16501 attacgttat tattgccacg cctaccgatt atgacccgaa aacaaattat tttaatacct
    16561 caagcgtgga agcggtcatc cgcgatgtta cagaaattaa tcccgacgct gtaatgatta
    16621 taaaatcaac tatccctgtt ggttttacag agtcaattaa agaacggttt ggtattgaaa
    16681 atgtgatctt ttcgccggag tttttacgcg aagggaaagc actttatgat aacttgcatc
    16741 catcacgcat tgtgattggc gagcaatccg accgcgcaga acgttttgcc gcgttattac
    16801 aggaaggcgc tattaagcaa gacataccaa cattgtttac tgactcaacc gaagctgagg
    16861 cgattaaact ttttgccaac acttatctgg ctatgcgtgt agcgtatttc aatgaacttg
    16921 atagttatgc tgaaagcctg gggcttaatt cacgccagat tattgagggc gtatgccttg
    16981 acccgcgtat cggtaatcac tacaacaacc cgtcattcgg ttatggtggt tattgtctgc
    17041 cgaaagatac caagcagtta ctagcaaatt accagtctgt gccgaataac ctgatctcgg
    17101 caattgttga cgccaaccgc acgcgcaaag attttattgc cgattctatc cttgcacgta
    17161 aaccgaaagt tgttggcgtc tatcgtttga ttatgaagag tggttcagac aatttccgtg
    17221 cttcctcgat tcagggtatt atgaagcgaa tcaaagcgaa aggcgtgcct gtaatcgttt
    17281 atgagccagc tatgaaagag gacgattttt tccggtcgcg cgtggtacgt gatctggatg
    17341 cattcaaaca agaagctgat gttattatat ctaaccgtat gtctgccgat ctggctgatg
    17401 tagcagataa agtttatacg cgcgacttgt ttggcaatga ttaattattt tgtttcattc
    17461 taagaaaagg ccctaataaa ttagggcctt ttcttatggt tttttaaaat caaactttat
    17521 agaagttacg ataccattca acaaagttct ttaccccctc tttaactgac gtctcaggtt
    17581 tgaatcctat tacgtcatac agtgcttttg tatcagcact ggtttctagt acatcaccgg
    17641 gttggagagg catcatattt ttgttggctt caatacccag agcctcttct aacgcattga
    17701 tatagtccat caactccaca ggcgaactat taccaatgtt atagacacga tatggtgctg
    17761 aacttgttgc aggcgagcct gtttctacag cccactgtgg atttttttct ggaataacat
    17821 cctgtaagcg aataatagct tcggcaatat catcaatata agtaaagtca cgcttcattt
    17881 tgccgaagtt gtaaacatca atgcttttac cttccagtat ggctttagtg aatttaaata
    17941 atgccatatc cggacgtccc catggaccat aaaccgtaaa gaaacgcagc cctgtggtcg
    18001 gtaagctata caaatgagaa tacgtatgag ccatgagttc attcgctttt ttagttgctg
    18061 cataaagcga aacaggatga tctacagagt catctgtaga gaaaggcatc ttgcggttca
    18121 tgccataaac agaactggag gaagcgtaaa gtagatgctg aacattatta tggcgacatc
    18181 cttccagtat gttcaggaat ccaatcaggt ttgcatctgc atatgcattg ggattttcaa
    18241 gggagtaacg tacaccggct tgcgcagcga ggtttattac gcgatcgaac cgctcgtctg
    18301 caaacagtgc cgccattttc tcacgatcgg ccaggtcaat tttataaaaa ctgaaattgt
    18361 cgtgcttgag taaatcaagt cgtgcttgtt tgaggttgac atcgtaataa tcatttaagt
    18421 tgtcaatccc tacaacctga tgaccagctg caagaagccg tttacttaca tagaaaccga
    18481 taaagccagc agctcccgta accagaaatt tcatttataa tcctcgctca ggctagaata
    18541 tagccaatct tcatctggca taactgaaag ttaaatcata ccgttagaca agaaaaaaag
    18601 ataatcggta tcagttctaa acctggctgt tttttctggt aacgtgctca ttttacaatc
    18661 aaagctgttc taagctgact atacaagccg acgtcattat ttccaaccgt atggcagaag
    18721 agcttaagga tgtggcagac aaagtctaca cccgcgatct ctttggcagt gactaacatc
    18781 ctgttatcag ggcgattttc gccctgattc tcttatgttc cctttgtaat cattcattat
    18841 ttttatcatt tatcctatag cattcacggg gattatcgct aaactatggc gacttaaaat
    18901 tatttcgtcc gttagggtaa tgatgagagt agaaaataat aatgtttctg ggcaaaacca
    18961 tgacccggaa cagattgatt tgattgattt actggtgcag ttgtggcgtg gcaagatgac
    19021 aattatcatc tctgtcattg ttgctattgc cctggctata gggtatctgg ccgttgccaa
    19081 agaaaagtgg acttccacag ctatcgttac tcaacctgat gtggggtaaa tcgctggtta
    19141 taccaacgcc ataaatgtga tttatggttc agctgcgccg aaagtgtctg agatccagtc
    19201 gatgttaatt ggtcgcttca gcacgacatt ttctgcatta gcagaaacgc tggataatca
    19261 ggaagaacct gaaaaactga ccattgagcc taccgttaaa aatcagtcat tacctttagc
    19321 ggtttcttat gtgggtgaaa cacctgaagg tgcgcagaaa cagctggcga agtatatcca
    19381 gcaagttgac gaccaggtaa atgaagaact ggaacaagac ctgaaagaca acatcgcgct
    19441 gcaaatgaaa aacttgcagg attctctgaa gacccaggaa gtggttgcgc aggagcagaa
    19501 agatctgcgt atctgtcaga ttcaggaagc gttgcagtat gcgaatcagg cgcaggtgac
    19561 aaagccgcag attcagcaga cacaggacgt aacccaagat actatgttcc ttttggggag
    19621 cgaagcgctg gagtcgatga ttaagcacga ggcgactcgt ccgttggtgt tctcatcaaa
    19681 ctactatcag actcgtcaaa acctgctgga tatcgacaat cttgacgttg ataaacttga
    19741 tattcatgct taccgctatg taatgaaacc gacgttacct attcgtcgcg atagcccgaa
    19801 aaaggcaatt accttgattc tggcggtgct gctgggcggc atggttggcg cggggattgt
    19861 gctggggcgt aacgctctgc gtaattacaa cgcgaagtaa tcttttcggt tttaaagaaa
    19921 aagggcaggg tggtgacacc ttgcccgttt tttttgccgg atgcgacaac aacatcgcat
    19981 ccgatcaccc gcaactcact gatgccgttt acgcagattt tcaattaccg tcgttaaatc
    20041 cagatcctga tcctgcaaca gcaccagcag gtgatacatc aagtcagatg cttcgttggt
    20101 cagttcaaag cggtcatgca ccgttgctgc cagtgcggtt tccacgcctt cttcgcccac
    20161 tttctgcgca atgcgtttag tgccgctggc atacagtttg gctgtgtaag aggtttccgg
    20221 gttggcagat ttacgttcgg cgagcagttg ttccagttga tacaggaaca gccactggtg
    20281 agcggtgtcg ccgaagcagc tgctggtgcc tttgtggcag gtcgggccga tgggattcac
    20341 cagtaccagt agcgtgtcgt tgtcgcagtc cggggcaatg ctcactacgt tgaggaaatt
    20401 gcccgacgtt tcgcctttgg tccacagtcg ctgtttagtg cgcgagaaga aggtgacttt
    20461 gccgctttcg atggttttgt ctaaggcttc cgggttcata tagcccagca ttaacacttc
    20521 gccggatacc gcatgttgca caatcaccgg cataagtccg tcggtttttt cccagtccag
    20581 ttcgcgacgt tgttgttctg ttaacatatc ctgatctcca cgccctgtgt tgccaggtac
    20641 gc
//
