LOCUS       LC177549               20130 bp    DNA     linear   BCT 15-DEC-2018
DEFINITION  Escherichia coli DNA, O-antigen biosynthesis gene cluster OgN5,
            strain: E705.
ACCESSION   LC177549
VERSION     LC177549.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., von Mentzer,A., Kikuchi,T. and Thomson,N.R.
  TITLE     An untypeable enterotoxigenic Escherichia coli represents one of
            the dominant types causing human disease
  JOURNAL   Microb Genom 3 (2017) In press
  REMARK    DOI:10.1099/mgen.0.000121
            Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20130)
  AUTHORS   Iguchi,A., von Mentzer,A. and Thomson,N.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2016) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuenkibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..20130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E705"
                     /db_xref="taxon:562"
                     /note="Escherichia coli O-antigen biosynthesis gene
                     cluster OgN5"
     gene            14..1408
                     /gene="wcaM"
     CDS             14..1408
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAV90454.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPVARALPARSSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRLMHNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            1571..2476
                     /gene="galF"
     CDS             1571..2476
                     /gene="galF"
                     /note="OgN5_1"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAV90455.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     CDS             2804..3406
                     /note="OgN5_2"
                     /codon_start=1
                     /gene="orf3"
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAV90456.1"
                     /translation="MLKKAVKYVLRKLQNKKIGYGTSIIDSEYSDSNWLGCNVSLTKS
                     SLGKYTYISDDSRIRNCKIGAFCSIGRNVKIGLGFHPTNYISTSPYLYRRSFLGMKGF
                     SNKDMFLEEDYRNTVVGNDVWIGDNVLICGGCTINDGVIIGAGSIVTRDLESYGIYAG
                     VPAKLIRKRECSYVNLDSIPSEWWELDDHELSYLIKKHNE"
     gene            3399..4619
                     /gene="wzx"
     CDS             3399..4619
                     /gene="wzx"
                     /note="OgN5_3"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAV90457.1"
                     /translation="MNDLKLSLIFGTLSRVITFVNQVLSVPLTIAIIGINEFTRFNVI
                     TAGIAWLITVGGCLLPSLVGDISRAKEDHNDIMISEKISSALTVMVIFIITVMIGYIF
                     FFGVMDNERNLLLIFSILILLFSTAENVRQGLGENYKNAIYNGCSNLLSLVIILGLAY
                     FEIKTNLQMVIIVMFGSITFFKLLNLLPLMRYFSLKYITFDSCKGMLDKATGFVFLSV
                     AYYFNTAGLITVLNWLNYTKLTEFIILQKIILIVMGMVVMIRNPLWGVIAKMQYKGNS
                     DLIIVNYRKVLRVYFYTAPLLVIISVLCIPFFLKMWAGGIVISYPDIIAFSIYLVSIV
                     FSYINSVLYYGLELFNKISRILIVESIVNIICVLFVALMGLKLNMIFIVMTITSLFIN
                     YFISKIIKRTCKYV"
     gene            4612..5799
                     /gene="wzy"
     CDS             4612..5799
                     /gene="wzy"
                     /note="OgN5_4"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAV90458.1"
                     /translation="MYKVENGFDLDELIYGIFISLLFFGYPIFSILATIFDQDVLTIF
                     YRGICIFIGLLAMVIPSKKFGFYASYSMVQILVISLHLIYLSWVLYYFSNGYRNENLD
                     LSFYVNNAILFSLIPVLLLNKKITSDTLIVLKKQFKFFILMFIALTFIAYTLGLSDPY
                     RLSFEKINPISLSLYAAICILFCNWSFRNKTISICVIILLLFIMVLSGSRGPLLALFL
                     VTCSSIFFRIKFIKKIYAVFTTAVLFFTIIVFYDNLVDYIPIISRFNLATTEGSLSVN
                     IREEQYKSAIEIFSNNPYLGGSLVEKFAFFYPHNIVLEILITGGIVLFLLYFLVFSAF
                     LVEIINALRNKIPICFIHVFFLLFVSYMFTSSLAGIGLLYFVIVIISKIKLDYKYDKY
                     NHC"
     CDS             5777..6514
                     /note="OgN5_5"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf6"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90459.1"
                     /translation="MINIITVNLNNVNGLEKTLSSLRSQDLSRDKWKLIVVDGMSTDG
                     SVELLKRHYDFIDKLVIEKDSGVYDAMNKGISYVNNADYFLFLNSGDAFVHSSVLSKL
                     NKIIMYSESNISVYYGDKINKCGELIKAYYPDSMQYGIINACHQCILYKKEKDIAFDL
                     RYKLFSDLDFTIKYYINNSKFQYVNIPISIYEDGGMSSIHQWKTKKEIYYILYRQFGF
                     VIFFRFLCVKLSKIFGIKHTFFLKKHS"
     CDS             6524..7408
                     /note="OgN5_6"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="hypothetical protein"
                     /protein_id="BAV90460.1"
                     /translation="MLKKIPFIVVHKGNQNYLKSCIFSLKKYHSTILLGNEENKSIIK
                     EWYDINSFNSEYYNRFDKCYVHMSSNSEVFEKNCFYRYIAMYEFAINKNITEFVHCDS
                     DIILLDDCTPLLDELSMFGAAYFIPDDQTNFRMTASPHFCYWKINVLKEFIEYFITEY
                     ETGASILKEKYKYHLKEKIPGGICDMTLLYLFSKQRKDIFNIFNHHKFYMNFNVATNE
                     STGCKRKDIIFNHQYKKIHNELFYYKKNSEEKKVLFLHMQGRTKKLMKYAESKKFKKL
                     FFLISLLNIIRRVQRLIY"
     CDS             7418..8554
                     /note="OgN5_7"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90461.1"
                     /translation="MKVAVVYHYFAHYREHVFEELIRIVNEKESTIQFYGDIHSNEPN
                     LNTYKFDNHKDKFTRVNNIWFGKFLWQSNLISKLFRDSPDTIIFLGQFNFISTWISAI
                     LFRLMGKRILFWGHGVYGSEKGLKRIVRDIFNRLPHIYMTYGEHAKELMVLSGIKESS
                     ISVIYNSLNVKQQEQFYEKLDNLSVDNLLDYHFDKTKTNIVFVGRLTKVKKIDLIFEA
                     LAKLGNRNYKLIIIGNGPEETHLKQAVKKLNLISMVTFMGEIHDESILSWLIYNSDIC
                     ISPGNVGLTAMHSLVYGTPVITNNDFCHQMPEYEAIDEEKNGGFFEANNIESLAQKIE
                     EWSNKILTTDRGLLRNECRKPILLKYNPKNQAQLIWECITKNVK"
     CDS             8544..9095
                     /note="OgN5_8"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="putative acetyltransferase"
                     /protein_id="BAV90462.1"
                     /translation="MLNKIKEIKYIYEVVRKIYTFFFKIKYGTPYIHSSARVLKPLYI
                     SKDFSLGCYSFINKGCYIGSKVVCGNFVMFGPYVTIAGGDHAFDLVGQPMYFSGRQNI
                     PTTIIGNDVWIGANSCIKAGVCIGEGAIVGMGSVVVKDVPAYAIVAGNPARLIKMRFN
                     ESQALKHSEKISDETKIIRNYCK"
     gene            9100..10224
                     /gene="gmd"
     CDS             9100..10224
                     /gene="gmd"
                     /note="OgN5_9"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="BAV90463.1"
                     /translation="MNMKKALITGITGQDGSYLAEFLLDKGYEVHGIKRRASSFNTER
                     VDHIYQDRHNQNPNFFLHYGDLTDTSNLVRLIKEIQPDEVYNLGAQSHVAVSFESPEY
                     TADVDAMGTLRLLEAIRICGLEKKTRFYQASTSELFGLVQEIPQRETTPFYPRSPYAV
                     AKMYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLY
                     LGNMDSLRDWGHAKDYVRMQWMMLQQDHPEDFVIATGKQISVREFVRMSAKEVGLELE
                     FSGQGVDEIATVVNKTSDCAIGVSIGDVIVRVDPRYFRPAEVETLLGDPAKAKKVLGW
                     EPEITVEEMCAEMVASDLAKAKQHALLKSHGYDVAVSLER"
     gene            10228..11193
                     /gene="fcl"
     CDS             10228..11193
                     /gene="fcl"
                     /note="OgN5_10"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthetase"
                     /protein_id="BAV90464.1"
                     /translation="MTKKRIYVAGHRGMVGSAICRQLSLRDDIELVVKTHKELDLTVQ
                     KDVDAFFAQEKIDQVYLAAAKVGGIYANNTFPAEFIYKNLMIESNIIHSAHKAGIQKL
                     LFLGSSCIYPKFAEQPMNESALLTGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGINDNFHPENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
                     AASVHVMELDEAIYQQNTQPMLSHINVGTGVDCSIREMAETMASVVGYQGKIVFDVTK
                     PDGTPRKLMDVTRLKNLGWQYRYNLHEGLSLTYKWFIENINSFRG"
     gene            11196..11657
                     /gene="gmm"
     CDS             11196..11657
                     /gene="gmm"
                     /note="OgN5_11"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="BAV90465.1"
                     /translation="MNKRLERELFKTIVEHTPLISIDLIIRNDKGEALLGQRLNRPAQ
                     NYWFVPGGRIYKDESFEIAFKRITLEELGVQISLNDALFLGVYEHFYNDNFFEAEFST
                     HYIVHGYEIQLKPQQLHLPTVQHNSYKWFDVVTLLNSTIVHQYTKNYFIPR"
     gene            11663..13069
                     /gene="manC"
     CDS             11663..13069
                     /gene="manC"
                     /note="OgN5_12"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="BAV90466.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
                     DKVEHKPALVICNESHRFIVAEQLRKESLKHSGILLEPVGRNTAPAVALAALQAMVTG
                     DDPILLVLAADHEIQDEDNFIAAVLAAKNFAEQGKLVTFGIVPTSPETGYGYIKSGES
                     LDGQGYKVAAFVEKPDLHVAQRYISDGGYLWNSGMFMFRASVFIDELKKFRPDILASC
                     QRSLSSSIQDLDFIRLDNASFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
                     WEISSKDQSGNAIRGDVLVEDATDSYLYSQHRLIGAVGVKDLVVVETKDAVLVAHKDK
                     VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGPRFQVKHVIVLPGHITAKQI
                     HYHRTEHWIVVSGTAKVHLEDKTYLVSENESTYIPVGVPHAIENPGKIPLEIIEVRSG
                     VYLEEDDVIRVSSSGVGY"
     CDS             13069..13815
                     /note="OgN5_13"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf14"
                     /product="glycosyltranslocase"
                     /protein_id="BAV90467.1"
                     /translation="MRISIITATYNSEKTLLDTLLSLEKQTHPDIEYIVVDGASKDNT
                     IKLIKRNCTKVSKIICEPDKGIYDALNKGIQAASGDVIGFLHSDDLLAYDDAIADIAK
                     KFKSTGCDAIYGDLEYVAQNDTTKRIRLWKSGSFSRFKMKVGWMPPHPSFYMKRECYS
                     QFGSFSLDYRISADYDSLLRYILKQRISIAYLPKVLVKMRVGGISNRSVSSMVKKSME
                     DIRIMKQNGIIWPLALAYKNISKLPQFIKK"
     gene            13821..15245
                     /gene="manB"
     CDS             13821..15245
                     /gene="manB"
                     /note="OgN5_14"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="BAV90468.1"
                     /translation="MLNVKKIINDSNIAFGTSGARGLVIDFSHDVCAAFTHAFLSVID
                     DKYNFNKVALAIDNRPSSYEIAQACAYAIKQHGFEVEYHGVIPTPALAHYSMQKNIPC
                     IMVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFSPVGVLPHLETSTQGADYY
                     LERYVSLFNPEILKGKRIGVYEHSSAGRDLYAPLFNQMGAEVISLGRSDEFVPIDTEA
                     VSDEDRIIAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKA
                     LAIPVSCNTAIEESNKFASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFLLASD
                     LQINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFASDSSQ
                     QIIKNAILSPNSFFNSLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPELRCYA
                     EASDENQAKQYVTNVLGNITSLIS"
     gene            15442..16848
                     /gene="gnd"
     CDS             15442..16848
                     /gene="gnd"
                     /note="OgN5_15"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAV90469.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELIAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGNKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            17070..18263
                     /gene="ugd"
     CDS             17070..18263
                     /gene="ugd"
                     /note="OgN5_16"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAV90470.1"
                     /translation="MFNRKKSELMKITISGTGYVGLSNGILIAQNHEVVALDIVQAKV
                     DMLNKKQSPIVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFN
                     TSSVEAVIRDVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYD
                     NLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVA
                     YFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQS
                     VPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRI
                     KAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRD
                     LFGSD"
     gene            18353..19387
                     /gene="wzz"
     CDS             18353..19387
                     /gene="wzz"
                     /note="OgN5_17"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAV90471.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(19484..20095)
                     /gene="hisI"
     CDS             complement(19484..20095)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAV90472.1"
                     /translation="MLTEQQRRKLDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKFADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     6083 a   3625 c   4286 g   6136 t
ORIGIN
        1 tagaggagaa cgcatgccat ttaaaaaact ctcccgacgc accttcctga cggcaagctc
       61 ggcgcttgct ttcctccata cccccgtcgc ccgcgcactt cctgcccgat caagcgttaa
      121 tatcaacgat tacaatccgc acgactggat cgcctcattt aaacaggctt ttagtgaagg
      181 gcaaacggtc gtcgtgcctg ccggattggt ttgtgacaat atcaacaccg gcatcttcat
      241 tcctgcaggt aaaacgttac acatccttgg aagcctgcgc ggcaatggca gagggcgatt
      301 tgtcttacag gacggcagcc aggtgacagg ggaggagggc ggcagtatgc ataacatcac
      361 cctggatgtg cgcggctctg actgcaccat caaagggctg gcgatgagcg gctttggccc
      421 ggtaacgcag atttatatcg gcggcaaaaa caaacggctc atgcacaacc tgaccatcga
      481 taacctgacc gttagccacg ctaattacgc catcttacgc cagggatttc ataaccagat
      541 tatcggtgcc aacatcacca actgtaagtt cagcgactta caaggcgacg ccattgaatg
      601 gaacgtggca attaacgaca gtgatatttt gatatctgac catgtcattg agcgcatcaa
      661 ctgtaccaac ggcaaaatca actggggcat cggcataggc cttgcgggga gtacttatga
      721 taataactac ccggaagacc aggcagtgaa aaactttgtc gtggcgaata tcacgggatc
      781 ggattgtcga cagttgatcc atgttgaaaa tggtaaacat tttgttattc gtaatatcaa
      841 agcccgcaat atcacgccgg atttcagtaa gaaagcaggt attgataacg ccacggtcgc
      901 tatttacggt tgtgacaatt tcgtgattga taatattgaa atgattaata gcgccgggat
      961 gttaatcggc tatggggtaa ttaaaggcaa atatctctcg ataccgcaaa atttccgagt
     1021 gaataatatt caactggata acacccacct tgcttataaa ttgcgcggca tccaaatctc
     1081 tgccgggaat gctgtctcct ttgtggcgct gactaacatt gagatgaagc gtgcgtcgct
     1141 ggagttacac aacaaaccgc aacatctttt tatgcgtaat atcaaggtga tgcaggaatc
     1201 ctcagttgga cccgcattga gcatgaactt cgacatgcgc aaagacgttc gcggcgtctt
     1261 tatggcgaaa aaagaaacac tgctgtctct tgcaaatgtt catgcggtga atgaaagagg
     1321 gcaaagctcc gtcgatatcg acaggattaa tcaccatatt gttaatgtgg aaaagattaa
     1381 ctttagattg ccggaacgga gagagtagat ttgcgaccat tcctggaaaa atggagccat
     1441 acttaggaac aatgctactg caatccacaa cgaagcggcg taacatcaca agtaattcag
     1501 taatcaattc agggtaattg atgctggcga aaaaaatcga acaagctata attcagcaac
     1561 cattttacag gtggatgaaa taatgacgaa tttaaaagca gttattcctg tagcgggtct
     1621 tgggatgcat atgttgcctg ccactaaggc gattcccaaa gagatgctac cgatcgtcga
     1681 caagccaatg attcagtaca ttgttgacga gattgtggct gcagggatca aagaaatcct
     1741 cctggtaact cacgcgtcca agaacgcggt cgaaaaccac ttcgacacct catatgaatt
     1801 agaatctctc cttgagcagc gcgtgaagcg tcaactgctt gcggaagtgc agtccatctg
     1861 tccaccgggc gtgaccatta tgaacgtgcg tcagggcgaa cctttaggtt taggccactc
     1921 cattttgtgt gcacgacccg ccattggtga caacccattt gtcgtggtgc tgccagacgt
     1981 tgtgatcgac gacgccagtg ccgatccgct gcgttacaac cttgctgcca tgattgcgcg
     2041 cttcaacgaa acgggccgca gccaggtgct ggcaaaacgt atgccgggtg acctctctga
     2101 atactccgtc attcagacca aagaaccgct ggatcgcgaa ggcaaagtca gccgcattgt
     2161 tgaatttatc gaaaaaccgg atcagccgca gacgctggac tcagacatca tggccgttgg
     2221 tcgctatgtg ctttctgcag atatttggcc ggaacttgaa cgcactcagc caggtgcatg
     2281 gggacgtatt cagctgactg atgccattgc cgaactggcg aaaaaacagt ctgttgatgc
     2341 catgctgatg acaggtgaca gctatgactg cggtaaaaaa atgggttata tgcaggcatt
     2401 tgtgaagtat ggactacgca acctgaaaga aggggcgaag ttccgtaaag ggattgagaa
     2461 gctgttaagc gaataatgaa aatctgaccg gatgtaacgg ttgataagaa aattataacg
     2521 gcagtgaaga ttcgtggcga aagtaatttg ttgcgaatat tcctgccgtt gttttatata
     2581 aacaatcaga ataacaacga gttagcaata ggattttagt caaagttttc caggattttc
     2641 cttgtttcca gggcggattg gtaagacaat tagcgtttga atttttcggg ttaagcgcga
     2701 gtgggtaacg ctcgtcacat cgtaggcatg catgcagtgc tctggtagct gtaaagccag
     2761 gggcggtagc gtgcattaat aaatagacgg tcagtgaaat aaaatgctta agaaagctgt
     2821 taagtacgtt ttgcgaaaat tgcaaaataa gaaaataggt tatggcacaa gtattatcga
     2881 cagtgaatac tcagatagta attggctggg ctgcaatgtt agcttaacaa aaagttcatt
     2941 aggtaaatat acttatatat cagatgactc aaggattaga aattgcaaga ttggtgcatt
     3001 ttgctcaatc ggcagaaatg taaaaattgg acttggcttt catcctacga attatatatc
     3061 aacaagtcct tatctttata gacgttcatt tcttggtatg aaaggctttt ctaataaaga
     3121 tatgttttta gaagaagatt atagaaatac ggtcgtagga aacgatgttt ggatcggtga
     3181 taatgttttg atatgtggag gttgtacaat aaatgatggt gtaattattg gagcaggaag
     3241 tattgttacg agagatttgg agtcatatgg catctatgct ggcgtacctg ctaaactaat
     3301 tagaaaaaga gaatgctctt atgttaacct ggacagtatt ccctccgaat ggtgggaact
     3361 tgatgatcat gaactaagct atttgataaa aaaacataat gaatgatctt aagttatcac
     3421 ttatatttgg taccctttcg agagtaataa cgttcgtaaa ccaagtttta tctgttccat
     3481 taactattgc aataattgga ataaatgaat ttacacgttt taatgtaata acggctggca
     3541 ttgcatggtt aatcacagtt ggaggatgct tacttccatc tttggtaggg gatatttcaa
     3601 gagctaaaga agatcataat gatattatga tttctgaaaa gatatcgtca gcactcacag
     3661 tgatggtgat ttttattatt actgtaatga ttggctatat ctttttcttt ggtgtgatgg
     3721 ataatgaaag aaatctacta ttaatatttt ccattctgat attgttattt tctacggctg
     3781 agaatgttcg gcaaggatta ggtgagaatt acaaaaatgc gatttataat ggatgttcaa
     3841 atttattatc gctggttata atattaggtc tcgcatattt tgaaattaaa acaaatcttc
     3901 aaatggttat aattgtcatg tttggcagta ttactttttt taaattgctg aatttgttac
     3961 cattaatgcg atacttttct ctgaaatata taacatttga tagttgcaaa ggaatgctag
     4021 ataaagcaac aggttttgtc tttctgtctg ttgcttatta ttttaataca gcaggattaa
     4081 taacagttct aaactggtta aattatacta aacttacaga gtttatcatt ctgcaaaaaa
     4141 taatattaat tgtgatgggg atggttgtaa tgatacgtaa tcctttatgg ggtgtcattg
     4201 caaaaatgca atacaaaggc aatagtgatt taataatagt gaactataga aaagtattaa
     4261 gagtttattt ctatactgca ccattgctag taattatttc agtgctatgc attccatttt
     4321 ttttaaagat gtgggctgga ggcattgtta tttcttatcc tgatattata gcattctcaa
     4381 tatatttagt ttcgattgtg ttttcatata taaactcagt gctatattat ggtttggaac
     4441 tatttaataa aatttcgagg attttgattg tagaatcaat cgttaatata atatgcgtat
     4501 tatttgttgc attaatgggg ctaaagttaa atatgatttt catagtgatg actatcacat
     4561 ctttgtttat aaattatttt atttccaaaa ttatcaagag aacatgtaaa tatgtataaa
     4621 gtagagaatg ggtttgatct tgatgagttg atttatggca tatttatttc tctactattt
     4681 tttggatacc ctatcttttc catattagca acaatttttg atcaagatgt gttaacgatt
     4741 ttttacagag gtatatgtat tttcattggc ttattggcta tggtaatacc atctaagaaa
     4801 tttggttttt atgcatcata ttcaatggtg caaattcttg taatatctct tcatctgatc
     4861 tatctatcat gggtattgta ttatttctct aatggatata ggaatgaaaa cctagatcta
     4921 tccttttacg taaacaatgc tattttattt tcactaattc ctgttttgtt gctaaataaa
     4981 aaaatcacat cagacactct aattgtttta aaaaaacagt ttaagttctt tattttgatg
     5041 ttcattgcat taacttttat cgcttatact ctaggtttaa gcgacccgta tcgattatct
     5101 ttcgaaaaaa taaatcctat ttcattaagc ttatatgcgg ctatttgtat tttgttttgc
     5161 aattggtcct tcagaaataa aacaatatca atttgcgtaa taattctatt actattcata
     5221 atggtattga gtggttctag ggggccatta ttagcactat ttttggttac atgcagttcg
     5281 attttcttta gaataaaatt tattaaaaaa atatatgctg tatttacaac agcagtttta
     5341 ttctttacta taatcgtatt ttacgacaat ctagtagatt atatccccat aatctcaagg
     5401 tttaatcttg ctacaacaga aggtagttta tcagttaata taagagaaga gcagtataaa
     5461 tcggccattg aaattttctc aaataatcca tatcttggtg gaagtttagt cgaaaaattt
     5521 gctttttttt acccccataa tatagttctt gagatactca taacaggtgg gattgtttta
     5581 tttctacttt attttctggt gtttagcgct ttccttgttg aaatcataaa tgctttgaga
     5641 aataaaatac caatttgttt tatacatgtg ttttttctac ttttcgtatc ttatatgttt
     5701 acctcatcac tggctggaat tggtttactt tattttgtaa ttgtaattat ttcaaaaata
     5761 aaactggatt ataaatatga taaatataat cactgttaac ttaaataatg ttaatggatt
     5821 agagaaaaca ttatcatcgc tacgttcaca agacttatca cgagataaat ggaagttaat
     5881 agttgttgat ggtatgtcta cagatggtag tgtagagtta ttaaaaagac attatgattt
     5941 cattgataaa ctcgttatag agaaagatag tggagtttat gacgcaatga ataaaggtat
     6001 atcatatgtt aataacgctg attattttct gtttttgaat tcaggagatg catttgtaca
     6061 ctcgtcagtt ttatcaaaat tgaataaaat aatcatgtat tcagaatcaa atatctctgt
     6121 ttattacggt gataaaatta ataaatgtgg agaattgatc aaagcatact atcctgatag
     6181 tatgcaatat gggattataa atgcttgcca tcagtgtatc ttatataaga aggaaaagga
     6241 tatagctttt gatttaagat ataagctttt ttcagattta gattttacta ttaaatatta
     6301 cattaataat tccaagtttc aatatgttaa tatacccatc tctatttatg aagatggtgg
     6361 tatgtctagt atccatcaat ggaaaacaaa aaaagaaata tactatatat tgtatagaca
     6421 gtttggattt gtgattttct ttaggtttct atgtgttaaa ttatctaaga tttttgggat
     6481 taaacataca ttctttttaa aaaaacatag ctgacaaaat tttatgctca agaaaatacc
     6541 atttatcgtt gttcacaagg gtaatcaaaa ttatcttaaa tcgtgcatat tctcgcttaa
     6601 aaaatatcat tcaactattt tacttggtaa cgaagaaaat aaaagtataa taaaagaatg
     6661 gtatgatata aactcattta atagcgaata ttataatagg tttgataaat gttatgtaca
     6721 tatgtcatcc aatagtgaag ttttcgaaaa aaattgtttt tatagatata ttgcaatgta
     6781 cgaatttgct ataaataaaa atattactga atttgtacac tgtgactctg atattatttt
     6841 attggacgat tgtactccat tgctggatga attatcaatg tttggggctg catattttat
     6901 acccgacgat cagactaatt ttcgtatgac tgcatcacca catttctgct actggaagat
     6961 taatgtatta aaagaattta ttgaatattt tattacagag tatgaaacag gggcatctat
     7021 cttaaaagaa aaatataaat atcacctaaa agaaaaaata cctggtggta tttgtgatat
     7081 gactttatta tatttattct ctaaacaacg taaggatata tttaatatct ttaatcatca
     7141 caaattttat atgaacttta acgtggcaac aaatgaatcc acaggctgca agcgtaagga
     7201 tattattttt aatcaccagt ataaaaaaat acacaacgag ctattttatt acaagaaaaa
     7261 tagtgaagaa aaaaaagtat tgtttctgca catgcaaggc aggactaaaa aattaatgaa
     7321 atatgcagaa agtaaaaaat tcaaaaaact tttttttctc atatcgttac ttaatataat
     7381 tcgtagagtt cagagattga tatattgagg tttaaaaatg aaggttgctg tagtctatca
     7441 ctattttgca cattataggg agcatgtttt tgaggagttg attcgtattg tcaacgagaa
     7501 agaatctacg attcaatttt atggagatat tcattcaaat gaaccaaatt taaatacata
     7561 taagtttgac aatcataaag ataagtttac aagagttaat aatatctggt ttggcaagtt
     7621 tttgtggcaa agtaatttga tatctaaatt atttagagac tcacctgata caattatatt
     7681 tttagggcag tttaatttta tttctacttg gatttctgct attttattca gattgatggg
     7741 gaaacggata ttattctggg ggcatggggt atatgggagt gaaaaaggac taaaaagaat
     7801 agttagggat atatttaatc gactgcctca tatttacatg acttatggag aacatgcgaa
     7861 agaattaatg gtattatcag ggatcaaaga atcaagtatt tctgttatat ataatagtct
     7921 aaatgttaaa cagcaagaac aattttacga aaagcttgat aatctttcgg ttgataactt
     7981 acttgattac cattttgata aaacaaaaac taatattgtc tttgtgggac ggttaacaaa
     8041 agtaaaaaaa atagatttaa tatttgaagc attagcaaag ttaggcaata gaaattataa
     8101 attaataatc ataggtaatg gtccagaaga gacacattta aaacaagctg ttaaaaaact
     8161 taatcttata tcgatggtta cttttatggg ggagatacat gatgaatcaa ttctttcttg
     8221 gcttatatac aatagcgata tttgtatatc accaggaaat gtcggtctaa ccgcaatgca
     8281 ttcattagtg tatggcactc cagttataac aaataatgat ttttgccacc agatgccgga
     8341 gtatgaagca atagatgaag aaaaaaatgg tggctttttt gaggctaata acattgagtc
     8401 tctagctcag aaaatcgaag aatggagtaa caaaattcta accacggatc gtggtttatt
     8461 acgcaatgaa tgtaggaagc ccatactctt aaagtataat cctaaaaatc aagcacaatt
     8521 gatttgggag tgcataacaa aaaatgttaa ataaaattaa agaaataaaa tatatatatg
     8581 aagttgtacg taagatttac acgttttttt ttaaaataaa gtatgggaca ccatacattc
     8641 actcaagtgc tagagtgtta aaacccttat atatttcaaa ggatttttca ttaggctgtt
     8701 actcttttat caataaagga tgctatatag gttctaaagt tgtttgtggt aacttcgtaa
     8761 tgtttggacc ttacgtgaca attgctggtg gagaccatgc ttttgatctt gttggacaac
     8821 caatgtattt ttcaggaaga caaaatatac cgaccactat tattggtaat gatgtatgga
     8881 ttggagctaa ctcatgtata aaagctggtg tttgtatagg tgagggtgcg attgtaggga
     8941 tgggaagtgt agtagtgaaa gatgtcccag catatgcaat tgtggcaggt aatccagcgc
     9001 gattaattaa aatgagattt aatgaatctc aagccttaaa acatagtgaa aaaatatctg
     9061 atgagacaaa aattatcaga aattactgta aataaaagga tgaatatgaa aaaagcatta
     9121 attacaggta ttacaggtca agatggttcc tacttggctg aattccttct tgataaaggg
     9181 tatgaagtgc atggcattaa acgtcgagca tcttcattta atacagagcg tgttgatcac
     9241 atttatcaag atcgccacaa tcaaaatcca aatttctttc ttcattacgg tgatttgaca
     9301 gatacttcaa acttggttcg cctcattaaa gaaatccaac cagacgaagt ttataacctt
     9361 ggcgctcaat ctcatgtagc ggtttcattc gaatcacctg aatacactgc tgacgtggat
     9421 gcgatgggga ctttacgtct gctggaagcg attcgtattt gtgggctaga gaaaaaaaca
     9481 cgtttctatc aggcatcaac ttctgaactc ttcggcttag ttcaggaaat cccgcagcga
     9541 gaaacaacac cattctaccc tcgttcgcct tatgctgttg cgaagatgta tgcatactgg
     9601 attactgtaa actatcgtga atcctatgga atgtacgcct gtaacggcat tctctttaac
     9661 catgaatccc cgcgtcgtgg cgaaacattc gtaacacgta aaatcacgcg tgctattgca
     9721 aatatttcgc agggaatcga aaaatgtctc tatcttggca atatggattc actgcgcgac
     9781 tgggggcatg cgaaagatta cgtgcgtatg cagtggatga tgttgcagca agatcaccca
     9841 gaggattttg taattgcaac aggcaaacaa atttccgtac gtgaatttgt tcgtatgtca
     9901 gctaaagaag tcggtctgga attagaattc tccggacaag gtgttgatga aatagcaact
     9961 gttgtgaata aaacatctga ctgtgctatt ggtgttagta ttggggatgt gatcgttcgt
    10021 gttgatcctc gttatttccg tcctgcagaa gttgaaactc ttcttggtga tccagcaaaa
    10081 gcgaaaaaag tattgggttg ggaaccagag attacagttg aagagatgtg tgcagaaatg
    10141 gttgccagtg atctggcgaa agcaaaacag catgcacttc tgaaaagcca tggttacgat
    10201 gttgcagttt ctctggagcg gtaaggtatg acaaaaaaac gtatctacgt tgctggtcac
    10261 cgaggtatgg ttggctctgc tatttgccgt caattatcac tgcgtgatga tatcgaatta
    10321 gtggtcaaaa cacacaaaga actcgatcta accgtacaga aggatgttga tgcatttttt
    10381 gcgcaagaga aaattgatca ggtttatctt gctgcggcta aagttggtgg catttatgcc
    10441 aacaatactt ttccggcaga attcatctat aagaatctca tgattgagag taatattatt
    10501 cattcagctc acaaggccgg aattcaaaaa ttactttttt taggctcaag ctgtatttat
    10561 cctaaattcg cagagcagcc gatgaacgag tcagcgcttt taacgggcat acttgagcca
    10621 actaatgaac catacgcaat tgctaaaatt gcgggcataa aattatgtga atcttataac
    10681 cgtcaatatg gccgtgatta tcgcagtgta atgcctacta atctttatgg cataaatgat
    10741 aatttccatc ctgaaaactc tcatgttatt ccggcactca tgcgtagatt ccatgaagca
    10801 aaagagagtg gtgcgccaga ggttattgtc tggggaaccg gaacaccgat gcgtgagttt
    10861 ttatatgttg atgatatggc tgccgcatcc gtccatgtaa tggagcttga cgaagcaatt
    10921 tatcaacaaa atacacagcc tatgttatcc catattaatg ttggtacggg tgtagattgt
    10981 tctatacgtg aaatggctga aacaatggcc tctgtggtgg gttatcaagg taaaattgtt
    11041 tttgatgtta ctaaacctga tggcactccg cgtaaactta tggacgttac ccggctcaaa
    11101 aacttgggct ggcaatatcg ctataattta catgaaggct tatcattaac atataaatgg
    11161 tttattgaga atattaattc ttttcgggga tagttatgaa caagagattg gaacgtgagc
    11221 tatttaaaac aatagttgag catactcctc taatctcgat tgatctcata attagaaacg
    11281 ataaaggaga ggcgctgctg gggcagcgcc tgaatcgccc agcacaaaat tattggtttg
    11341 tgccaggagg acgaatttat aaggatgagt cattcgagat tgcatttaag cggataacac
    11401 ttgaagagtt gggcgttcaa attagtctta atgatgcctt atttcttggg gtgtatgaac
    11461 atttttacaa tgataatttt tttgaagcag aattttctac acactatata gtgcatggat
    11521 atgaaatcca acttaaacct cagcaacttc acctaccaac ggtccagcat aattcctaca
    11581 agtggtttga tgtagtaacg ttgcttaata gcactatagt tcatcaatat accaaaaatt
    11641 attttatacc aaggtaatag atatgctact tcccgttgtc atggccggtg gttctggtac
    11701 cagattatgg cctctttcac gtacacttta tccgaaacaa tttctgtctt taaatagtcg
    11761 tttaaccatg ttgcaagaga cattgcggcg gcttgacaag gtcgaacata aacctgcttt
    11821 ggtcatttgt aacgaatcac atcgctttat cgttgctgaa caattgcgta aagagagttt
    11881 aaagcatagc ggtattttgc ttgagcctgt tggtcgtaat actgcgcctg ctgtagcact
    11941 tgcagcactt caggctatgg taactggaga cgatcctatt cttttggttc ttgctgctga
    12001 tcatgaaatc caggatgagg ataatttcat tgctgcagtt ctcgctgcaa agaattttgc
    12061 agagcagggt aagcttgtta cgtttggtat tgttccaaca tccccagaga ctggctacgg
    12121 ttacattaag tcaggtgaat ctctggatgg acaaggttac aaagttgcgg cttttgttga
    12181 aaaaccagat cttcacgtag ctcagcggta catatcagat ggcggttatc tttggaatag
    12241 cgggatgttt atgttcagag catctgtatt tatcgatgaa ctgaaaaaat tccgaccaga
    12301 tattttagcc agttgccaac gctccttgtc ctcttcgata caagatttag attttatccg
    12361 tctggataat gcttcatttt cttgctgtcc tgaagagtct attgactatg cagttatgga
    12421 aaaaacagca gaagctgtcg tcgttccatt aaatgcacaa tggagtgatg ttgggtcatg
    12481 gtctgcattg tgggaaataa gttcaaaaga tcaaagtggt aatgccattc gtggtgatgt
    12541 attggttgaa gatgctacag atagttatct ttattcgcag catagactta ttggtgccgt
    12601 gggcgtaaag gatttggttg ttgttgaaac gaaagatgcc gtattagttg ctcataaaga
    12661 taaagttcag caagttaaaa acatcgtcgc tcaacttaaa aagaataatc gcacagaata
    12721 tttacagcat cgggaaatat ttcggccttg gggcagtcat gatactatag ctgaagggcc
    12781 gcgctttcaa gtgaaacatg tgattgtatt acctggtcac attaccgcta agcagattca
    12841 ttaccaccgt actgaacatt ggattgttgt ctcggggaca gctaaagttc atcttgaaga
    12901 taagacttac cttgtttctg aaaatgaatc aacatatata cctgttggtg ttccacatgc
    12961 cattgaaaat cctggcaaga tcccgcttga aataattgag gttagatcag gagtctactt
    13021 ggaagaggat gatgttataa gagtttcttc ttctggagta ggatactaat gcgaatttct
    13081 attatcacag ccacttataa tagtgaaaaa actctccttg atacattact ttctctagaa
    13141 aaacaaacgc atccagatat cgaatatata gttgtagatg gagcatcgaa agataataca
    13201 attaagctga tcaagaggaa ttgtacaaaa gtttcaaaaa tcatttgcga acccgataag
    13261 ggcatttatg atgctctgaa taaaggtatt caagcagctt cgggtgatgt tattggtttt
    13321 ttacattctg atgatttact agcttatgat gatgctattg cagatatagc aaaaaaattt
    13381 aaaagtacag ggtgtgatgc tatttatggc gatttggagt atgttgccca aaatgatacg
    13441 actaaacgta ttagattatg gaaaagtggt tcatttagtc gtttcaagat gaaagtgggt
    13501 tggatgccgc cacacccatc attttatatg aaacgcgaat gttatagtca gtttggtagt
    13561 ttttctttag attatcgaat atctgctgat tatgactcat tattacgata tattttaaaa
    13621 caacgtattt cgatagcata tttaccaaaa gtcttagtga agatgcgtgt tggtggaatt
    13681 agcaatcgtt cagtatcttc aatggtcaag aagtcaatgg aagacattcg tattatgaaa
    13741 cagaatggta ttatctggcc acttgcttta gcgtataaaa atatatccaa acttcctcaa
    13801 tttattaaaa agtaatcatc atgttaaatg ttaaaaaaat cattaacgat agtaatattg
    13861 cgttcggtac tagtggtgct cgcggtttag tgatcgattt ttcccatgat gtttgtgctg
    13921 cgttcactca tgcgtttctt tctgttattg atgacaaata caattttaat aaagttgcct
    13981 tagccattga taaccgccca agtagttacg aaattgctca ggcatgcgcg tatgctataa
    14041 aacaacatgg attcgaggtt gaatatcatg gtgtaattcc gactcctgca ttagctcatt
    14101 attcgatgca gaaaaacatt ccctgcataa tggtcacagg gagtcatatc ccttttgatc
    14161 gtaatggctt aaaattctac agacctgatg gtgaaatcac taaagaggat gagctcgcaa
    14221 ttataaatag tgagtataca ttttcgcctg taggtgtatt acctcatctt gaaacaagca
    14281 ctcaaggtgc agactactac ctcgagcgtt atgtttctct ttttaatccc gagattttaa
    14341 aggggaaaag aataggggta tatgaacatt ctagtgcggg acgcgattta tatgctcctc
    14401 tttttaatca aatgggtgca gaggtcattt ccctcggtag gagtgatgag ttcgttccga
    14461 ttgatacgga agcagtaagc gatgaagatc gtataattgc aagagagtgg tctaaaaaat
    14521 ataatctcga cgctattttc tcaacagatg gcgatggtga tcgtccttta gttgccgatg
    14581 aaaatggtga atggttaaga ggcgatattc tgggactact tactgctatt gaacttaata
    14641 tcaaggcgtt ggctattcca gtgagttgta atacagcaat tgaagagtct aataaatttg
    14701 caagtataca acgaacgaaa ataggttctc cttatgtaat tgcagcattt gcagatcttg
    14761 ctaagcaatt tgattcagtc gctggttttg aagctaatgg tggttttctc cttgcctccg
    14821 atttacaaat taatggcaag gaattaaaat cgttacctac acgagatgct gtgttaccag
    14881 cattaatgct cttaatagct tctcgcaata gtactatttc tcaactgatt aataatcttc
    14941 ctcagcgatt cacttggtca gatagagtta aaaacttcgc ttcagattca agtcaacaaa
    15001 ttataaaaaa tgccatattg tcacccaata gtttctttaa tagtttaggt tatgaatcat
    15061 tatcctgttc cgctattgat gaaacggatg gtgcaagatt tactttaaat aatggtgata
    15121 ttatacatct ccgtccttcc ggtaatgctc cagaactccg ttgttatgct gaggccagtg
    15181 atgaaaatca ggctaagcaa tatgttacga atgtgctggg aaatattacc tctttgattt
    15241 cttgatgtta taggtttatc tacgcttata tgtgtgcgta ggtttgatta cacgtagatg
    15301 ccggtataca gaattgaaga acggtatttg ttgcattaat gaaattcagc actacacaca
    15361 ttcgtgcaac ttgagataac atctcaatca tattcaagtc gcgcatacat cgcggtgaac
    15421 atcccctgac aggagtaaac aatgtcaaag caacagatcg gcgtcgtcgg tatggcagtg
    15481 atggggcgca accttgcgct caacatcgaa agccgtggtt ataccgtctc tattttcaac
    15541 cgttcccgtg aaaagacgga agaagtaatt gctgaaaatc caggcaagaa actggttcct
    15601 tactatacgg tgaaagagtt tgttgaatct ctggaaacgc ctcgtcgcat cctgttaatg
    15661 gtgaaagcag gtgcaggcac ggatgctgct attgattctc tcaagccata cctcgataaa
    15721 ggcgacatca tcattgatgg tggtaacacc ttcttccagg acaccattcg tcgtaaccgt
    15781 gagctttctg cagaaggctt taactttatc ggtaccggtg tttccggtgg tgaggagggc
    15841 gcactaaaag gtccttccat tatgcctggt gggcagaaag aagcctatga actgattgcg
    15901 ccgatcctga ccaaaatcgc cgcagtggct gaagacggtg agccatgcgt tacctatatt
    15961 ggtgccgatg gcgcaggtca ctatgtgaag atggttcaca acggtattga atacggcgat
    16021 atgcagctga ttgctgaagc ctattctctg cttaaaggtg gtctgaacct caccaacgaa
    16081 gaactggcgc agacctttac cgagtggaat aacggtgaac tgagcagcta cctgatcgac
    16141 attaccaaag acatcttcac taaaaaagat gaagacggta actacctggt tgatgtgatc
    16201 ctggatgaag cggcaaacaa aggtacgggc aaatggacca gccagagcgc actggatctc
    16261 ggcgaaccgc tgtcgctgat taccgagtct gtgtttgcac gatacatctc ttctctgaaa
    16321 gatcagcgcg ttgctgcgtc taaagttctc tctggcccac aagcgcagcc agctggcaac
    16381 aaggctgagt tcatcgaaaa agttcgccgt gcactgtatc tgggcaaaat cgtttcttac
    16441 gctcaggggt tctctcaact gcgtgcggcg tctgaagagt acaactggga tctgaactac
    16501 ggcgaaatcg cgaagatttt ccgtgctggc tgcatcatcc gtgcgcagtt cctgcagaaa
    16561 atcaccgatg catatgccga aaatccgcag atcgctaacc tgctgctggc cccgtacttc
    16621 aagcaaatcg ccgatgacta ccagcaggcg ctgcgcgatg tcgtcgctta cgcagtacag
    16681 aacggtatcc cggttccgac cttcgccgct gcggttgcct attatgacag ctaccgtgcc
    16741 gctgttctac ctgcgaacct gatccaggca cagcgtgact atttcggtgc gcatacttat
    16801 aagcgcattg ataaagaagg tgtgttccat actgaatggc tggattaatc tgatttaaat
    16861 caattaacca acacaaggcc cggagaaacc ctccgggctt ttttattata caaagcggca
    16921 ggttatggcc tttttttata atttatgatt aaaaaagcga tataatacag agctgcacag
    16981 caggatcgct gtcttgacag ttcaaccgca tcagcgttaa aaatcccgca gtagatgaag
    17041 ttgtggtggt ggactaatga ccactctaaa tgtttaaccg gaagaagtca gagctaatga
    17101 aaataacaat ttcaggaaca ggttatgttg gtctttcaaa tggtattctg attgcgcaaa
    17161 accacgaagt ggttgcactg gatatcgttc aggccaaagt ggacatgctt aacaagaagc
    17221 agtcaccgat tgttgataag gagattgaag agtatctggc gactaaagat ctcaatttcc
    17281 gcgctacgac agataagtat gacgcgtata aaaatgccga ttacgttatt attgccacac
    17341 ctaccgatta tgacccgaaa acaaattatt ttaatacctc aagcgtggaa gcggtcattc
    17401 gcgatgtgac agaaattaat cctaatgctg tcatggtgat taaatctaca attccggtag
    17461 gtttcaccaa atcaattaaa gaacgtctgg gtatcgataa ccttattttc tctccggaat
    17521 tcctccgcga aggtaaagcc ctttacgata atctccatcc ttcacgtatt gtcattggtg
    17581 agcgttcaga acgcgcagaa cgttttgctg cgttgttaca ggaaggcgcg attaagcaaa
    17641 atatcccgac cctgtttacc gactccactg aagcagaagc gataaaactg tttgctaata
    17701 cctatctggc gatgcgcgta gcatacttta atgaactgga tagctatgca gaaagtttag
    17761 gtctgaatac tcgccagatt atcgaaggcg tttgtctcga tccgcgtatt ggcaaccatt
    17821 acaacaatcc gtcgtttggt tatggtggtt attgtctgcc gaaagatacc aagcagttac
    17881 tggcgaacta ccagtctgtg ccgaataacc tgatttcggc aattgtcgat gcaaaccgaa
    17941 cgcgtaaaga ttttattgcc gatgccattt tgtcacgcaa gccgcaagtg gtgggtattt
    18001 atcgtctgat tatgaagagc ggttcagata acttccgcgc gtcttccatt caggggatta
    18061 tgaagcgtat caaggcgaaa ggcgttgaag tgatcatata cgagccagtg atgaaagaag
    18121 actcattctt caactctcgc ctggaacgtg atctcgccac cttcaaacaa caagccgacg
    18181 tcattatctc caaccgtatg gcagaagagc ttaaggatgt ggcagataag gtatacaccc
    18241 gcgatctctt tggcagcgac taacatcatg ttatcagggc gattttcgcc ctgattctct
    18301 tatgttccct ttgtaataat tcattatttt tatcatttat cctatagcat tcatggcgat
    18361 tatcgctaaa ctatggcgac ttaaaattat ttcgtccgtt agggtaatga tgagagtaga
    18421 aaataataat gtttctgggc aaaaccatga cccggaacag attgatttga ttgatttact
    18481 ggtgcagttg tggcgtggca agatgacaat tatcatctct gtcattgttg ctattgccct
    18541 ggctataggg tatctggccg ttgccaaaga aaagtggact tccacagcta tcgttactca
    18601 acctgatgtg gggcaaatcg ctggttatac caacgcgatg aatgtgatct acggatcggc
    18661 tgtaccgaaa gtctccgaca ttcaggcttc acttatcgga cgttacagca ctgcattctc
    18721 agcattagcg gaaacgctgg ataaccagga agaagcagaa aagctgacca ttgagcctac
    18781 cgttaaaaat cagtcattac ctttagcggt gtcttatgtt ggtgaaacac ctgaaggtgc
    18841 gcagaagcag ctggcgaagt atatccagca agttgacgat caggtaaatg aagaactgga
    18901 aaaagacctg aaagacaaca tcgcgctgcg tatgaaaaac ttgcaggatt ctctgaaaac
    18961 tcaggaagta gtcgcgcagg agcagaaaga tctgcgtatc cgtcagattc aggaagcgtt
    19021 gcagtatgcg aatcaggcgc aggtgacaaa gccacaggtt cagcagactg aagatgtgac
    19081 gcaagatacg ttgttccttt tggggagcga agcgctggag tcgatgatta agcatgaggc
    19141 gacccgtccg ttggtgttct caccaaacta ctatcagaca cgtcaaaacc tgctggatat
    19201 cgaaagctta aaagttgatg atcttgatat tcatgcttac cgctatgtaa tgaaaccgac
    19261 gttacctatt cgtcgcgata gtccgaaaaa ggcaattacc ttgattctgg cagtgctgct
    19321 gggtggcatg gttggcgcgg ggattgtgtt ggggcgtaat gctctgcgta attacaacgc
    19381 gaagtaatct ttgcagcttt aaagaaaacg ggcagggtgg tgacaccttg cccgtttttt
    19441 ttgccggatg cgacaacaac atcgcatccg cttaccccgc aactcactga tgccgtttac
    19501 gcaggttctc aattactgtc gttaaatcca gatcctgatc ctgcaacagc accaacaggt
    19561 gatacatcaa atcagatgcc tcgttggtca gctcaaagcg gtcatgtaca gtcgcggcca
    19621 gtgcggtttc cacgccttct tcgcccactt tctgcgcaat gcgtttggtg ccgctggcat
    19681 acagtttggc agtgtaagag gtttccgggt cggcaaattt gcgttcggcg agcagttgtt
    19741 ccagttgata caggaacagc cactggtgag cggtgtcgcc gaagcagctg ctggtgcctt
    19801 tgtggcaggt cgggccgatg ggattcgcca gcaacagtaa cgtgtcgttg tcgcagtccg
    19861 gcgtaatatt tactacgttg aggaaattgc ccgacgtttc gcctttggtc cacagtcgct
    19921 gtttagtgcg cgagaagaag gtgactttgc cgctttcgat ggttttgtct aaggcttccg
    19981 ggttcatgta gcccagcatt aacacttcgc cggataccgc gtgttgcaca atcaccggca
    20041 tcagtccgtc ggttttttcc cagtccagtt tgcgacgttg ttgttctgtt aacatatcct
    20101 gatctccacg ccctgtgttg ccaggtacgc
//
