LOCUS       LC125928               21220 bp    DNA     linear   BCT 04-JUN-2016
DEFINITION  Escherichia coli DNA, O-antigen biosynthesis gene cluster, strain:
            102755.
ACCESSION   LC125928
VERSION     LC125928.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Seto,K., Nishii,H., Ohnishi,M., Mekata,H.,
            Ogura,Y. and Hayashi,T.
  TITLE     Six Novel O Genotypes from Shiga Toxin-Producing Escherichia coli
  JOURNAL   Front Microbiol 7, 765 (2016)
   PUBMED   27242776
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21220)
  AUTHORS   Iguchi,A., Iyoda,S. and Seto,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2016) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuenkibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..21220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="102755"
                     /db_xref="taxon:562"
                     /note="O genotype:OgN8; serogroup:O141"
     gene            468..1862
                     /gene="wcaM"
     CDS             468..1862
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAU71565.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGKGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NDKINWGIGIGLSGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEREQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            2025..2930
                     /gene="galF"
     CDS             2025..2930
                     /gene="galF"
                     /note="OgN8_1"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAU71566.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            3302..4387
                     /gene="rmlB"
     CDS             3302..4387
                     /gene="rmlB"
                     /note="OgN8_2"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAU71567.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSKRYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIHKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            4387..5286
                     /gene="rmlD"
     CDS             4387..5286
                     /gene="rmlD"
                     /note="OgN8_3"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="BAU71568.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLINATSVEAIAKAANEVGAWAIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTVI"
     gene            5344..6222
                     /gene="rmlA"
     CDS             5344..6222
                     /gene="rmlA"
                     /note="OgN8_4"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAU71569.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVDMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEKVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            6227..6775
                     /gene="rmlC"
     CDS             6227..6775
                     /gene="rmlC"
                     /note="OgN8_5"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="BAU71570.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKWSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFFVLSEIAEFLYKTTNYYNPESERSIHWMDPELNISWPNT
                     SELIISEKDKKALSFSNCESMN"
     gene            6783..8063
                     /gene="wzx"
     CDS             6783..8063
                     /gene="wzx"
                     /note="OgN8_6"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAU71571.1"
                     /translation="MRRLILNNKFLKNVAIIASGTALAQLINLAAIPLITRLYGPEAY
                     GMMGSFMSIVNVLIPLSALSYPIAVVLPRKDTVSNIIVRLSLILGIATSLIALIGLVL
                     YKLLKNDSGIPLWYIAVIPLTILILPLQQCGQQWLIRKKQYSALAKISVIQSIVVNSL
                     KILIGLPLHGVKTLVIITALGYWLQALQFFHNAKKNGLIIITSKFVRNLPKVSRAYSD
                     FPIYRTPQVLVNALSQSLPVFVIGYYFGVTQVGYFSLAQTILGAPVTLLSGALSNVLF
                     PKISQKVNNRELISPLIKKSFLGLITLSTSIYMTVILFGPQLFSFVFGQAWEIGGVYG
                     RWMALYCIFWLSARPAIDSIPSLKIQRQFLIYEVCSLLLRFTGLITGVMIYNDSICAM
                     ILYSIINALCYFSLIMFVLWKCKKHEQKVFRTSN"
     gene            8032..9201
                     /gene="wzy"
     CDS             8032..9201
                     /gene="wzy"
                     /note="OgN8_7"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAU71572.1"
                     /translation="MSRKYLEPVIRDESIQFQENNNSKLANTIINNSFFGICIIAVSF
                     RVLIIAVIMLAACALWSLLTLKASMKTFAMMGGILLFLCLYYFLGLSHYERGPEKFTN
                     AFSLILFSFVFFFILGYKETVEGRIKWLNFFSLCSIIYVITVCIYSILKGYPGYNLVY
                     DPINRSEENSPLYALQLVLFSIAYVNFNWSKQRRFFNIALILIATFFSVLYLGSRAAF
                     FLIIIFFTLKIITRKHSLLLFFVLIIMAALIIMNFASDLNITNSFNFGGFSERGLDSP
                     RFSMFIYGISNFLDYPFGGLKVHAEGYTGIWFHNMLLDLVRVSGYYVMFLWLIILIVT
                     TCFLMKFNDKKYIILFIIVNVAFMQDLAFDGFFNLMALEFYLIGASLVKAQKFNV"
     CDS             9194..10336
                     /note="OgN8_8"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="glycosyltranslocase"
                     /protein_id="BAU71573.1"
                     /translation="MFEKILFVNESPVYGGHEEMFLRHINEIAKKRHLYLNIVVNVKN
                     KRLIDEVDNLAKINSNINLNVFRHRFWGLPLRPITNFLCVKDFCWLLKTFVSFKPEKI
                     ILIQGTIEIGGLSLLAARISGKYTSTYLPITKKSKDIGVAFGSMRDFINKHFYYQLPH
                     EIMTISEFNKEELINNFAVDDYRIKVVKNFIDAPSYPVQKGSEEENNILLIIGRIDLN
                     QKRQDLFLDFFVKSELSDFFEVHIIGDGSDEVSQTVRNKYNGMHNVYFCGWLSSNDVV
                     QRLAQCRCVIIPSKFEGVPLVMIEAIKLGKIVIASNVDGMKEYLPPQWLFDVNEMSDS
                     LRIISYLNEKPKVFEDLLPVVQKKFNQIFDPIENSKNFMNLINNKK"
     CDS             10340..11371
                     /note="OgN8_9"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="glycosyltranslocase"
                     /protein_id="BAU71574.1"
                     /translation="MNILLDCRLISNRPTGISRYSEKMLEYYIDKYGMDNIHALVNQK
                     SDKIKCKQIVTTLKPFNIVHWLIFPLWLKKNNYHWIISFHYSGIAYRIENVNSVITVH
                     DLMFELVPNFFNTTLKNLIGKLYYRIIVKKSIMNSDITISVSETTRNDILRLYKKDSK
                     ISGEGIFLQAKSDESLLMKYNLESNNYFLYIGNNRPHKNIDFLKKCFQDAKLKNNLNC
                     KLVLVGHAGQSDDDIIYPGILSDEQIVGLYRNAKAFVFPSKYEGFGLPILEALDNKCP
                     VVASDIPAFREFSNNNITYFKLGDKAALADVFNRDYKFDDAEAQEVINRYSWPQTYKN
                     LDRIFGGLI"
     CDS             11368..12450
                     /note="OgN8_10"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /product="glycosyltranslocase"
                     /protein_id="BAU71575.1"
                     /translation="MKDIIIVHDFDGRPYFKAIEKNFQCKFINSRPIRFLIRDIVKKR
                     TITKDTIDSILFLFLLPFHKNQTIILGMAPFNFRLLFYGMLCYRNKVLLHSSWHQWLG
                     RTPFSYINIFNKIFKKLWRHYLPRFERIIAVTKATKQSILDFSYPLSLNIDVIPHVVD
                     IEPITRAELDKKWNSKCIQGLFVGRLTPEKGIKDIINISEKMQSGLLKDSNIKIAGKG
                     YLEDDIVHASKRISGLLYMGYITSRDKLRDLMKQSQFFLLPSKKIKGWEELFGLVIVE
                     AMSQGCVVIASNHIGPKEIIDNHKDGILCEESDFVDNTLRNFNNFHTDIYYYKEISMR
                     ALDKSQKYNMANISQLWVELINNIER"
     CDS             12459..13229
                     /note="OgN8_11"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf12"
                     /product="glycosyltranslocase"
                     /protein_id="BAU71576.1"
                     /translation="MTVFISVVSHGHAEIIKKLDCLASLATIFKVVIKCNKSGDAELL
                     SEYENCQNIHILNEAYGLGFGKNNNYVFDYCLKHLGMNENDYFIVLNPDVKIHLKTLE
                     KLIRIMNIDDSRLASINLYKNSEMTIFDNSVRKFPRFIDFVSSFVLKKNNVIYDKKKL
                     SVPVNVEWAAGSFLAFKVSHYALLNGFSDKYFMYCEDIDICYRSHLYNVPVRYYPEVK
                     AIHFAKHANRSIFSKHFWWHVSSIVKFLYFKYNNRIER"
     gene            13244..14611
                     /gene="manC"
     CDS             13244..14611
                     /gene="manC"
                     /note="OgN8_12"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="BAU71577.1"
                     /translation="MAGGSGTRLWPLSRSFYPKQFLELFSEDSLIQGTLNRLNDKDYG
                     TPIIITNNEYRFLVKEQIKKTKHPDSQIILEPVARNTAPAIAISALISIKDGADPLLL
                     VMPSDHIIKNEKQFSESIKKAREYAEKGYLVTFGINPTSPETGYGYIKASSSVGDGIF
                     GIERFVEKPSLNKAVEYISDGHYSWNSGIFLFKASTFLQELNSFCPEIYESCLKSVNS
                     AITDLGFIRVDPVSFANCPSDSIDFAVMELTSKGVVIPVDIGWSDVGSWSALWEVGNK
                     DNNNNVSKGDIYLDETSNSYIYSKERFIACIGVDDIIVVDTSDALLVVSKNNVQKVKD
                     VVNYLMVNNRTEYQNHNEQFRPWGSHEVVSSGSKYNIKRVVIKPGHSSSEQVHYNRTE
                     HWIVLSGTALVMCGDEIKTIKENESTFIPAGVKHNFSNPGKIDLEIIEVQTGPYLAED
                     DIYRD"
     gene            14619..15989
                     /gene="manB"
     CDS             14619..15989
                     /gene="manB"
                     /note="OgN8_13"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="BAU71578.1"
                     /translation="MQKLTCFKAYDIRGKLGEEMNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPNGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGHVPLMEEKTKLI
                     LELLSK"
     gene            16153..17559
                     /gene="gnd"
     CDS             16153..17559
                     /gene="gnd"
                     /note="OgN8_14"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAU71579.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            17781..18974
                     /gene="ugd"
     CDS             17781..18974
                     /gene="ugd"
                     /note="OgN8_15"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAU71580.1"
                     /translation="MFNRKKSELMKITISGTGYVGLSNGILIAQNHEVVALDIVQAKV
                     DMLNKKQSPIVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFN
                     TSSVEAVIRDVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYD
                     NLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVA
                     YFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQS
                     VPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRI
                     KAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRD
                     LFGSD"
     gene            19064..20098
                     /gene="wzz"
     CDS             19064..20098
                     /gene="wzz"
                     /note="OgN8_16"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAU71581.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGS
                     AAPKVSEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAQYIQQVDDQVNEELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGCMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(20194..20805)
                     /gene="hisI"
     CDS             complement(20194..20805)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAU71582.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     6199 a   4101 c   4756 g   6164 t
ORIGIN
        1 ctatcgcatc ctcggcattg gcccgtggga acgacgcctg cgcacgctca tcgaacaata
       61 tcaactggaa gatgtggtgg agatgcctgg ctttaaaccg agccatgaag tgaaagcgat
      121 gctcgacgac gcggatgtct tcctgttgcc atcggttaca ggtgcggatg gtgatatgga
      181 aggcattccg gtggcgctaa tggaagcgat ggcggtcggc attccggtgg tttctactct
      241 gcatagcgga ataccggaac tggtggaggc tgacaaatcc ggctggctgg tgcctgagaa
      301 cgatgctcgc gcactggcgc aacgcctggc ggcatttagc caactggaca ccaacgaact
      361 ggctccggtt atcaaacgtg ctcgcgaaaa agtcgaacac gattttaacc agcaggtgat
      421 taatcgagaa ctcgccagct tgttgcaggc tttatagagg agaacgtatg ccatttaaaa
      481 aactctcccg acgcaccttc ctgacggcaa gctcggccct tgccttcctc catacccctt
      541 tcgctcgcgc acttcccgcc cgacaaagcg ttaacattaa cgactacaac cctcacgact
      601 ggatcgcctc atttaaacaa gccttcagcg aagggcaaac ggtcgtcgtg cctgccggat
      661 tggtttgcga caatatcaac accggcatct tcattcctgc aggtaaaacg ttacacatcc
      721 ttggaagcct gcgcggcaac ggcaaagggc gatttgtttt acaggacggc agccaggtga
      781 caggggagga gggcggcagt atgcataaca tcaccctgga tgtgcgcggc tctgactgca
      841 ccatcaaagg gctggcgatg agcggctttg gcccggtaac gcagatttat atcggcggca
      901 aaaacaaacg ggtcatgcgc aacctgacca tcgataacct cactgtcagc cacgctaatt
      961 acgccatctt acgccaggga tttcataacc agattatcgg tgccaacatc accaattgta
     1021 agttcagcga cttacagggg gacgctattg aatggaacgt ggcaattaac gacagtgata
     1081 ttttgatctc cgaccatgtc atcgagcgca tcaactgtac taacgacaaa atcaactggg
     1141 gcatcggcat aggtctttcg ggtagtacct acgataacaa ctacccggaa gaccaggcag
     1201 tgaaaaactt tgtcgtggcg aatatcacgg gatcggattg tcggcagttg atccatgttg
     1261 aaaatggtaa acattttgtt attcgtaata tcaaagcccg caatatcaca ccggatttca
     1321 gtaagaaagc aggtattgat aacgccacag tcgctattta cggttgtgac aatttcgtga
     1381 ttgataatat tgaaatgatt aatagcgccg ggatgttaat cggctatggg gtaattaaag
     1441 gcaaatatct ctcgataccg caaaatttcc gagtgaataa tattcaactg gataataccc
     1501 accttgctta taaattgcgc ggcatccaaa tctccgccgg gaatgccgtc tcctttgtgt
     1561 cgctaactaa cattgagatg aagcgtgcct cgcttgagct tcacaataaa ccgcaacacc
     1621 tttttatgcg aaatatcaat gtgatgcagg aatcctctgt tggaccagca ttgagcatga
     1681 acttcgacat gcgcaaagac gtacgaggtg tctttatggc gaaaaaagaa acactcctgt
     1741 ctcttgcaaa tgttcatgcg gtgaatgaaa gagaacaaag ctccgtcgat atcgacagga
     1801 ttaatcacca tattgttaat gtggaaaaga ttaactttag attgccggaa cggagggagt
     1861 agatttgcga ccattcctgg aaaaatggag ccatacttag gaacaatgct actgcaatcc
     1921 acaacgaagc ggcgtaacat cacaagtaat tcagtaatca attcagggta attgatgctg
     1981 gcgaaaaaaa tcgaacaagc tataattcag caaccatttt acaggtggat gaaataatga
     2041 cgaatttaaa agcagttatt cctgtagcgg gtcttgggat gcatatgttg cctgccacta
     2101 aggcgattcc caaagagatg ctacccatcg tcgacaagcc aatgattcag tacattgttg
     2161 acgagattgt ggctgcaggg atcaaagaaa tcctcctggt aactcatgcg tccaagaacg
     2221 cggtcgaaaa ccacttcgac acctcttatg aattagaatc tctccttgag cagcgcgtga
     2281 agcgtcaact gcttgcggaa gtgcagtcca tctgtccacc gggcgtgacc attatgaacg
     2341 tgcgtcaggg cgaaccttta ggtttgggcc actccatttt atgtgcacga cccgccattg
     2401 gtgacaatcc atttgtcgtg gtgctgccag acgttgtgat cgacgacgcc agcgccgacc
     2461 cgctgcgcta caaccttgct gccatgattg cacgattcaa cgaaacgggt cgtagccagg
     2521 tgctggcaaa acgtatgccg ggtgacctct ctgaatactc ggtcatccag accaaagaac
     2581 cactggaccg tgaaggtaaa gtcagccgca ttgttgaatt tatcgaaaaa ccggatcagc
     2641 cgcagacgtt ggactcagac atcatggccg ttggtcgcta tgtgctttct gccgatattt
     2701 ggccggaact tgaacgcact cagcctggtg catgggggcg tattcagctg actgatgcca
     2761 tcgctgaact ggcgaaaaaa cagtccgttg atgccatgct gatgacaggt gacagctacg
     2821 actgcggtaa aaaaatgggt tatatgcagg catttgtgaa gtatggacta cgcaacctga
     2881 aagaaggggc gaagttccgt aaagggattg agaagctgtt aagcgaataa tgaaaatctg
     2941 accgaatgta acggttgata agaaaattat tacggcagtg aagattcgtg gcgaaagtaa
     3001 tttgttgcga attttcctgc cgttgtttta tataaacaat cagaataaca acgagttagc
     3061 aataggattt tagtcaaagt tttccaggat tttccttgtt tccagagcgg attggtaaga
     3121 caattagcgt ttgaattttt cgggtttagc gcgagtgggt aacgctcgtc acatcgtgga
     3181 catgtatgca gtgctctggt agctgtaaag ccaggggcgg tagcgtgcat taatacctct
     3241 attaatcaaa ctgagagccg cttatttcac agcatgctct gaagtaatat ggaataataa
     3301 agtgaagata cttgttactg gtggcgcagg atttattggt tctgctgtag ttcgtcacat
     3361 tataaataat acgcaggata gtgttgttaa tgtcgataaa ttaacgtacg ccggaaacct
     3421 agaatcactt gcagatgttt ctgactctaa acgctatgtt tttgaacatg cggatatttg
     3481 cgatgctgct gcaatggcgc ggatttttgc tcagcatcag ccggatgcag tgatgcacct
     3541 ggctgctgaa agccatgtgg atcgttcaat tacaggccct gcggcattta ttgaaaccaa
     3601 tattgttggt acttatgtcc ttttggaagc ggctcgcaat tactggtctg ctcttgatgg
     3661 cgacaagaaa aatagcttcc gttttcatca tatttctact gacgaagtct atggtgattt
     3721 gcctcatcct gacgaagtaa ataataaaga acaattaccc ctctttactg agacgacagc
     3781 ttacgcgcct agtagtcctt attccgcatc aaaagcatcc agcgatcatt tagtccgtgc
     3841 gtggaaacgt acctatggtt taccgaccat tgtgactaac tgttcgaata actacggtcc
     3901 ttatcacttt ccggaaaaat tgattccact agtaattctt aatgctctgg aaggtaaggc
     3961 attacctatt tatggcaaag gggatcaaat tcgtgactgg ctgtatgttg aagatcatgc
     4021 gcgtgcgtta tataccgtcg taaccgaagg taaagcgggt gaaacttata acattggtgg
     4081 acacaacgaa aagaaaaaca tcgatgtagt gctcactatt tgtgatttgt tggatgagat
     4141 tgtaccgaaa gagaaatctt accgcgagca aattacctat gttgccgatc gcccgggaca
     4201 cgatcgtcgt tatgcgattg atgcagagaa gattagccgc gaattgggct ggaaaccgca
     4261 ggaaacgttt gagagcggga ttcataaaac ggtggaatgg tacctgtcca atacaaaatg
     4321 ggttgataat gtgaaaagtg gtgcctatca atcgtggatt gaacagaatt atgagggacg
     4381 ccagtaatga atatcctcct tttcggcaaa acagggcagg taggttggga actacagcgt
     4441 gctctggcac ctctgggtaa tttgattgct cttgatgttc actccactga ttactgtggt
     4501 gattttagta atcctgaggg tgtagctgaa accgtaagaa gcattcggcc tgatattatt
     4561 gtcaacgcag ccgctcacac cgcagtagac aaagcagaat cagaaccgga gtttgcacaa
     4621 ttaattaacg cgacgagtgt cgaagcgatc gcgaaagcag ccaatgaagt cggcgcctgg
     4681 gctattcact actctactga ctacgtattt ccgggaacag gtgaaatacc atggcaggag
     4741 gcggatgcaa ccgcaccgct gaacgtttat ggagaaacca agttagccgg agaaaaagca
     4801 ttacaagagc attgtgcgaa gcaccttatt ttccggacca gctgggtcta tgcaggtaaa
     4861 ggaaataact tcgccaaaac gatgttgcgt ctggcaaaag agcgcgaaga actagccgtt
     4921 attaatgatc agtttggtgc gccaacgggc gcagagttgc tggctgattg tacggcacat
     4981 gcaattcgtg tggcactgaa taaaccagaa gtcgcaggtt tgtatcatct ggtagccagt
     5041 ggtaccacaa cttggcacga ttatgctgcg ctggtttttg aagaggcgcg caaagcaggc
     5101 attccccttg cactcaacaa gctcaatgca gtaccaacaa cagcctatcc tacaccagcc
     5161 cgtcgccccc ataactctcg tcttaataca gaaaaatttc agcagaactt tgcgcttgtc
     5221 ttgcctgact ggcaggttgg tgtgaaacga atgctcaacg aattatttac gacgacagta
     5281 atttaatagt ttttgcatct tgttcgtgat ggtggagcaa gatgaattaa aaggaatgat
     5341 gaaatgaaaa cgcgtaaagg tattatttta gcgggtggtt ctggtacacg tctttatcct
     5401 gtgactatgg ctgtcagtaa acagctatta cctatttatg ataagccgat gatctattac
     5461 ccgctttcta cactgatgtt agcgggtatt cgcgatatac tgattatcag tacgccacag
     5521 gatactcctc gttttcaaca actgctgggt gacggtagcc agtgggggct aaatcttcag
     5581 tacaaagtgc aaccgagtcc ggatggtctt gcgcaggcgt ttattatcgg tgaagagttt
     5641 attggtggtg atgattgtgc tttggtactt ggtgataata tcttctacgg tcacgacctg
     5701 cctaagttaa tggatgccgc tgttaacaaa gaaagtggtg caacggtatt tgcctatcac
     5761 gttaatgatc ctgaacgcta tggtgtcgtt gagtttgata aaaacggtac ggcgatcagc
     5821 ctggaagaaa aaccgctaca accaaaaagt aattatgcgg taaccgggct ttatttttat
     5881 gataacgacg ttgtcgacat ggcgaaaaat cttaagcctt ctgcccgcgg tgaactggaa
     5941 attaccgata ttaaccgtat ctatatggaa caagggcgtt tatctgttgc catgatgggg
     6001 cgtggttatg cgtggttaga cacggggaca catcagagcc tgattgaggc aagcaacttt
     6061 attgcaacaa ttgaagagcg tcaggggttg aaagtttcct gcccggaaga aattgcttac
     6121 cgtaaagggt ttattgatgc tgagaaggtg aaagtattag ctgaaccgct gaaaaaaaat
     6181 gcttatggtc agtatctgct gaaaatgatt aaaggttatt aataaaatga atgtaattaa
     6241 aacagaaatt ccagatgtac tgatttttga accgaaagtt tttggtgatg agcgtggttt
     6301 cttttttgag agttttaacc aaaaggtttt tgaggaagca gtaggtcgca aagttgagtt
     6361 tgttcaggat aaccattcga agtctagtaa aggtgtttta cgcgggctgc attatcaatt
     6421 agaaccttat gctcaaggga aattggtacg ttgcgttgtt ggcgaggttt ttgatgttgc
     6481 ggttgatatt cgtaaatggt cacctacctt tggcaaatgg gttggggtga atttatctgc
     6541 tgaaaataag cgccagttgt ggataccaga ggggtttgca catggatttt ttgtgctgag
     6601 tgagatagct gagtttttgt ataagacgac aaattattac aatcccgaaa gtgaacgaag
     6661 tattcattgg atggatccag agttaaatat ttcttggcct aatacatctg aactaattat
     6721 ttcagaaaag gacaaaaagg cattatcttt cagtaattgt gaaagcatga actaacaaga
     6781 aaatgcgaag attaatatta aacaataaat ttttaaaaaa cgtcgcgata attgcatcgg
     6841 gtacagcact tgcacagcta attaacttag ctgctattcc tcttatcact cgtctttatg
     6901 gcccagaagc gtacgggatg atgggaagct ttatgtcgat tgtaaacgta ttgattcctt
     6961 tatcagcatt aagctacccc attgctgttg tgttacctag aaaagatact gtttcaaaca
     7021 taattgtacg gttaagtttg atacttggaa ttgctacatc gttgatagct ctaataggat
     7081 tagttttata caagcttctt aaaaatgatt ctggaatacc attatggtat atagctgtaa
     7141 ttccactaac tatacttatt cttcctcttc aacaatgtgg gcagcaatgg cttataagaa
     7201 aaaaacaata tagtgcactt gccaaaattt cagttattca gtctattgta gttaactcat
     7261 taaaaatatt aattggatta cctttgcatg gtgttaaaac tttagtcatc ataactgctt
     7321 tagggtattg gttacaggca ttacaatttt tccacaacgc aaaaaaaaat ggacttatta
     7381 taattactag taaatttgta cgtaacttgc caaaggtaag tcgtgcttat tccgactttc
     7441 caatatatag aactccacag gttttggtta atgcactttc acagagtttg ccagttttcg
     7501 taataggata ttattttggg gttacgcagg ttggttattt tagtttggcg caaacaatac
     7561 ttggcgctcc tgttacgttg ctctctggtg cgttaagtaa tgttttattt cctaagattt
     7621 cacagaaagt taacaataga gaattaattt caccattaat taaaaagtct tttttggggc
     7681 ttatcacttt atcaacgtcg atatatatga cggtaatatt gtttggccca caattgtttt
     7741 cttttgtttt tgggcaagca tgggaaattg gtggtgtcta tggcagatgg atggctcttt
     7801 attgtatttt ttggctttct gccagacctg ctatagattc gattccgtca ctaaaaatac
     7861 aacgtcaatt tttaatatat gaagtatgct cgcttttgtt aaggtttact ggtttgatta
     7921 ccggtgtaat gatttataac gatagtatct gtgctatgat attatactct ataataaatg
     7981 cactgtgtta tttttctttg ataatgtttg tattatggaa atgtaaaaaa catgagcaga
     8041 aagtatttag aaccagtaat tagagatgaa agcatacagt ttcaggagaa taataattca
     8101 aaactggcaa atacaattat aaataattcg ttttttggaa tttgtattat agccgtttct
     8161 ttccgcgtat tgattattgc agtgatcatg ctagcagctt gtgctttatg gtctttattg
     8221 acattaaaag catccatgaa aaccttcgct atgatggggg ggatactgct atttttatgt
     8281 ttatattatt ttttgggatt atctcattat gaaagggggc ctgaaaaatt cacaaatgca
     8341 ttttcattaa ttttattttc atttgttttt ttcttcatat tagggtataa agaaactgtt
     8401 gaaggaagaa ttaaatggtt gaatttcttt tctctgtgta gtatcattta tgttataaca
     8461 gtctgtatat atagcattct caaaggatat cctgggtata atcttgttta tgatcctatc
     8521 aatagaagcg aagagaattc tccactttac gcccttcagt tagttctttt cagtattgct
     8581 tatgtgaatt ttaattggtc aaagcaacgt agatttttca atatagcttt aatactcatt
     8641 gctacttttt ttagtgtgct ttatctcggt tcgagagcgg ctttttttct aataataatt
     8701 ttttttacat taaaaataat aaccaggaaa catagcttat tgctgttctt tgtattaatt
     8761 attatggctg cactaataat aatgaacttt gctagcgatc tgaatattac taattcattc
     8821 aattttggtg ggttttctga gcgaggtctt gatagtccaa gatttagtat gtttatttat
     8881 ggcatatcca attttttaga ttaccctttt ggtggattaa aagttcatgc tgaaggttat
     8941 acaggtattt ggttccataa tatgctttta gatttagtca gagtatcagg ctattatgtt
     9001 atgttcttat ggcttattat ccttatagta accacctgtt ttttaatgaa atttaatgat
     9061 aaaaaatata ttatactttt cattatagta aatgttgcgt tcatgcaaga tttagcattc
     9121 gatggatttt ttaatttgat ggcattggag ttttatttaa tcggagcatc cctggtaaag
     9181 gcgcaaaaat ttaatgtttg aaaaaatatt atttgtaaat gagtctcctg tctatggcgg
     9241 gcatgaggag atgtttctac gtcatatcaa tgaaatagct aaaaaaagac atttgtattt
     9301 aaacatagtt gtaaatgtaa aaaataaacg cctaatagat gaagtcgata atttggcgaa
     9361 aataaattcc aacatcaatt tgaatgtttt tcgacatcga ttttggggct tgccactaag
     9421 accaataacg aactttttat gtgtaaagga tttttgttgg ctattgaaaa catttgtttc
     9481 tttcaaacct gaaaaaataa tattaattca aggaacaatc gaaataggtg gcttatcatt
     9541 attagcagca aggatatctg gaaaatacac ttctacatat cttcctataa ctaaaaaaag
     9601 taaagatatt ggagttgcat ttggtagtat gagagatttt ataaataagc atttctacta
     9661 tcagttacct catgaaatta tgacaatttc agaatttaac aaagaagaat tgattaataa
     9721 ttttgctgta gatgattata ggataaaggt ggtcaagaat ttcatagatg ccccttcata
     9781 tccggtacaa aaaggtagcg aagaagaaaa caatatttta ttaattattg gtaggattga
     9841 tctaaatcaa aaaaggcaag atttattcct cgattttttc gtaaaaagtg aattgtctga
     9901 ttttttcgag gtacacataa ttggtgatgg gagcgacgag gtttcacaga ctgttagaaa
     9961 taaatataac ggtatgcata atgtttattt ctgcggttgg ttgagttcaa atgatgttgt
    10021 tcaacgtctg gcccagtgca gatgtgtaat tattccttct aaatttgaag gtgtgccgct
    10081 tgttatgata gaggctatca aattaggaaa aatagttata gcaagcaatg ttgatgggat
    10141 gaaagaatat ttacccccac aatggttatt cgacgtcaac gaaatgagtg attcactacg
    10201 aattatttca tatttgaatg aaaaaccaaa agtgtttgag gatttactac cggtggtaca
    10261 aaaaaaattt aatcaaattt tcgatccgat cgaaaattct aaaaatttta tgaacctaat
    10321 caataataaa aagtgactaa tgaatattct tcttgactgt aggttaatta gtaatcgtcc
    10381 aactggtatt tcaagatatt cagagaaaat gttggaatat tacattgata aatatggtat
    10441 ggataacatc catgcattag taaaccagaa aagcgataaa ataaaatgta aacaaattgt
    10501 taccaccctt aaaccattta atatagtcca ttggttaatt tttcctttgt ggctcaaaaa
    10561 aaacaattac cattggatca tcagttttca ttactctggt attgcatacc gtatagaaaa
    10621 tgtaaattct gtaataacag tacatgattt aatgttcgag ttggtgccca atttcttcaa
    10681 cactacatta aaaaatctta ttgggaagct ttattaccgc ataatcgtaa aaaaaagcat
    10741 aatgaattca gatataacta taagcgtatc tgaaacaacc agaaatgata tactgaggtt
    10801 atataaaaag gattcaaaaa taagcggtga aggtatattt cttcaagcaa aaagtgatga
    10861 aagcttatta atgaagtata atctggaatc taacaactat tttctatata tagggaataa
    10921 tagacctcat aaaaatatag attttttaaa gaaatgtttc caggacgcca agttaaagaa
    10981 taatttaaac tgtaaattgg ttttagttgg acatgccggg caaagtgatg atgatataat
    11041 atatcctgga atattaagtg atgagcaaat tgttggactt tatagaaatg ctaaggcgtt
    11101 tgttttccct tcaaagtatg agggctttgg cctgcctatt ttagaggctt tagataataa
    11161 atgtccagtt gtcgcatcag atattcctgc attcagagag ttttcaaaca ataatataac
    11221 ttattttaaa ttgggagata aggccgctct ggcagatgta ttcaatcgtg actataaatt
    11281 tgatgatgcc gaagctcagg aagttattaa cagatatagt tggccacaaa cttataaaaa
    11341 tttagatcga atttttggag gcctgatatg aaagatatca ttatcgttca tgattttgat
    11401 ggtagaccat atttcaaagc aattgagaaa aattttcagt gtaaatttat aaatagtagg
    11461 ccaataaggt tcttgatacg tgatattgta aaaaagagaa caattactaa agacactatt
    11521 gattctattc tgtttttatt tcttctgcct tttcataaaa accaaactat aattttgggg
    11581 atggcaccat ttaattttag attgttgttc tatggtatgc tttgctaccg caacaaagtc
    11641 ctcctgcatt cctcatggca tcaatggttg ggaagaactc cgttttcata tattaatatt
    11701 ttcaataaaa tttttaaaaa attatggcgt cattatcttc ctcgttttga acggataatt
    11761 gctgtaacta aagcaactaa acagtcaata ttggattttt cctaccccct gtcactaaat
    11821 atagacgtga taccacatgt agtggatatt gaacctatta ccagagctga gttagacaaa
    11881 aagtggaatt caaaatgtat tcaagggtta tttgttggcc gactaacacc tgaaaaaggt
    11941 attaaagata taatcaatat ttcagagaaa atgcaatcgg gtttattgaa agattcaaat
    12001 atcaagattg ctggaaaagg atatcttgag gatgacatcg ttcacgcgag taaacgtatt
    12061 tctgggttat tgtatatggg atatataact tcacgggata aacttaggga tctcatgaaa
    12121 caatctcagt tttttttact tccttcaaaa aaaataaaag gttgggaaga attatttggt
    12181 ttggttattg tagaggctat gagtcaagga tgtgttgtaa tagcttctaa ccatataggc
    12241 cctaaagaaa ttatagataa tcataaagat ggtatccttt gcgaagaaag tgactttgta
    12301 gataatacat taagaaactt taataatttc catacagata tttattatta taaagaaatc
    12361 tcgatgagag cacttgataa aagtcaaaaa tacaatatgg cgaatatttc tcaactttgg
    12421 gttgaactca ttaataatat agaacgataa ggctttatat gaccgttttt attagtgttg
    12481 tatcacatgg acatgcagag attataaaaa aactagattg tcttgcatca cttgcaacta
    12541 tttttaaagt tgttattaaa tgcaacaaga gcggtgatgc tgaattgctt tctgaatatg
    12601 aaaattgcca aaacattcat attctcaatg aagcatatgg cttggggttt ggtaaaaata
    12661 ataattacgt attcgactat tgtctaaaac atcttggcat gaatgaaaat gattatttta
    12721 ttgttttaaa tccggatgtg aaaattcatc tcaagactct tgaaaagtta atcaggatta
    12781 tgaatattga tgatagtaga ctagcttcga ttaatctgta taaaaactct gagatgacta
    12841 tttttgacaa ttcagtaagg aaatttccta ggtttataga ttttgtttca tcattcgttc
    12901 taaaaaaaaa caatgtaata tatgataaaa aaaagttatc tgtcccagtt aatgttgaat
    12961 gggcagcagg ttctttttta gcatttaaag taagtcatta tgccttgctt aacggttttt
    13021 cagataaata tttcatgtat tgtgaagata ttgatatatg ttatcgctcc catctttata
    13081 acgtcccagt gagatattat cccgaggtta aagctatcca ttttgcgaaa catgcgaaca
    13141 gaagcatctt ttctaagcat ttttggtggc acgtaagtag tattgtgaaa tttttatact
    13201 ttaaatataa taacaggatt gaaagatgat tataccaata ataatggctg gaggtagcgg
    13261 tactcgcctt tggcctctct ctcgctcatt ttatccaaag caatttctcg agctgttctc
    13321 tgaggatagt ttaatacagg gtacacttaa tcgcttaaat gataaagatt atgggactcc
    13381 gataatcata acaaataatg agtatcgttt tttggttaag gaacaaataa aaaaaaccaa
    13441 gcaccctgat tcgcaaataa tactagagcc agtcgcaagg aataccgctc cagcaatagc
    13501 aatttctgca ttaatttcaa ttaaggatgg agccgatcct cttttgttag taatgccgtc
    13561 agatcatata atcaaaaatg aaaagcaatt ttcagaaagt ataaaaaaag cacgtgaata
    13621 tgctgaaaag ggctatttag ttacatttgg tatcaatcct acctcgccag aaacgggata
    13681 tggatatatt aaagcaagta gttctgtcgg tgacggtata tttggcatag aacgctttgt
    13741 tgagaaacct agccttaata aagccgttga atatatttct gatggtcatt atagctggaa
    13801 tagcggaatt tttcttttta aagcttctac tttccttcaa gaattaaatt ctttttgtcc
    13861 agaaatctat gaatcctgct taaagtctgt taatagtgca atcactgatc ttggtttcat
    13921 aagagtagat cctgttagtt tcgctaattg cccctctgat tcaattgatt ttgcagtaat
    13981 ggaattgacc agtaaaggtg ttgttatacc tgtagatata ggttggagtg atgtaggttc
    14041 ttggagtgca ctatgggaag ttggcaataa agataataat aataatgtct ctaagggaga
    14101 tatctatttg gatgagactt ccaatagtta tatttattct aaagaaagat ttattgcatg
    14161 tatcggtgtc gatgatatta ttgttgtaga tacaagtgac gcattactgg tagtttcaaa
    14221 aaataatgtg caaaaggtca aggatgtagt aaattatctt atggtaaata acagaacaga
    14281 atatcagaat cataatgaac agttccggcc ttggggaagt catgaagttg tgagttctgg
    14341 tagtaaatat aatattaaac gagttgtaat aaagcccgga catagcagtt ctgagcaagt
    14401 tcattataat agaacggaac attggattgt tctttcaggc acagctctag tcatgtgtgg
    14461 tgatgaaata aaaacaatca aagaaaatga atccactttt attccagctg gagttaaaca
    14521 taatttttct aacccaggaa aaattgattt agaaattatt gaggtgcaaa caggcccata
    14581 cctagctgaa gatgatatat atagagattg atattataat gcaaaagtta acctgtttta
    14641 aagcgtacga tattcgcggc aagctgggcg aagaaatgaa tgaagatatc gcctggcgta
    14701 ttggtcgcgc ttatggcgaa tttctcaaac cgaaaaccat tgtgttaggc ggtgatgtcc
    14761 gcctcaccag cgaaacctta aaactggcgc tggcgaaagg tttacaggat gcgggcgttg
    14821 acgtgctgga tattggtatg tccggcaccg aagagatcta tttcgccacg ttccatctcg
    14881 gcgtggatgg cggcattgaa gttaccgcca gccataatcc gatggattac aacggcatga
    14941 aactggtgcg cgaaggggct cgcccgatca gcggtgatac cggactgcgc gacgtccagc
    15001 gtctggcaga agccaacgac tttcctcccg tcgatgaaac caaacgcggt cgctatcagc
    15061 aaatcaacct gcgtgacgct tacgttgatc acctgttcgg ttatatcaat gtcaaaaacc
    15121 tcacgccgct caagctggtg attaactccg ggaatggcgc agcgggtccg gtggtggacg
    15181 ccattgaagc ccgctttaaa gcccttggcg cacccgtgga attaatcaaa gtgcacaaca
    15241 cgccgaacgg caatttcccc aacggtattc ctaacccgct actgccggaa tgccgcgacg
    15301 acacccgcaa tgcggtcatc aaacacggcg cggatatggg cattgccttt gatggcgatt
    15361 ttgaccgctg cttcctgttt gacgaaaaag ggcagtttat cgaaggctac tacattgtcg
    15421 gcctgctggc agaagcgttc ctcgaaaaaa atcccggcgc gaagatcatc cacgatccac
    15481 gtctctcctg gaataccgtt gatgtggtga ccgccgcggg cggcactccg gtgatgtcga
    15541 aaaccggaca cgcctttatt aaagaacgga tgcgcaagga agacgccatc tacggtggtg
    15601 aaatgagcgc ccaccattac ttccgtgatt tcgcttactg cgacagcggc atgatcccgt
    15661 ggctgctggt cgccgaactg gtgtgtctga aaggaaaaac gctgggcgaa ctggtgcgcg
    15721 accggatggc agcgtttccg gcaagcggtg agatcaacag caaactggcg caacccgttg
    15781 aggcgattaa ccgcgtcgaa cagcatttta gccgcgaggc gctggcggtg gatcgcaccg
    15841 atggcatcag catgaccttt gccgactggc gctttaacct gcgcacctcc aacaccgaac
    15901 cggtggtacg gttgaatgtg gaatcgcgcg gtcatgtgcc gctgatggaa gaaaaaacaa
    15961 aacttatcct tgagttactg agtaagtaat ccagtaattt catataaatg ggttttaaaa
    16021 gacggaaaag atgagatatc cagtgtggta tagccaaggt aatgctattc agcatctcta
    16081 tgagtgagtt aacatctata ccacatttaa gccgcacact cggcggtgac caccccctga
    16141 caggagtaaa caatgtcaaa gcaacagatc ggcgtcgtcg gtatggcagt gatggggcgc
    16201 aaccttgcgc tcaacatcga aagccgtggt tataccgtct ctattttcaa ccgttcccgt
    16261 gaaaagacgg aagaagtgat tgccgaaaat ccaggcaaga aactggttcc ttactatacg
    16321 gtgaaagagt ttgttgaatc tctggaaacg cctcgtcgca tcctgttaat ggtgaaagca
    16381 ggtgcaggca cggatgctgc tattgattcc ctcaagccat acctcgataa aggcgacatc
    16441 atcattgatg gtggtaacac cttcttccag gacaccatcc gtcgtaaccg tgagctttct
    16501 gcagaaggct ttaacttcat tggtaccggt gtctccggtg gtgaagaagg cgcgctgaaa
    16561 ggtccttcca ttatgcctgg tgggcagaaa gaagcctatg aactggttgc gccgatcctg
    16621 accaaaatcg ccgcagtggc tgaagacggc gaaccgtgcg ttacctatat tggtgccgat
    16681 ggcgcaggtc actatgtgaa gatggttcac aacggtattg agtacggcga tatgcagctg
    16741 attgctgaag cctattctct gcttaaaggt ggcctgaacc tcaccaacga agaattggcg
    16801 cagacgttta ccgagtggaa taacggtgaa ctaagcagct acctgatcga catcaccaaa
    16861 gatatcttca ccaaaaaaga tgaagacggt aactacttgg ttgatgtgat tctggatgaa
    16921 gcagcaaaca aaggtactgg caaatggacc agccagagtg cgctggatct cggtgaaccg
    16981 ctgtcgctga ttaccgagtc tgtgtttgca cgttatatct cttctctgaa agatcagcgc
    17041 gttgccgcgt ctaaagttct ctctggcccg caagcgcagc cagctggcga caaggctgag
    17101 ttcatcgaaa aagttcgtcg tgcgctgtat ctgggcaaaa tcgtttctta cgctcagggg
    17161 ttctctcaac tgcgtgcggc gtctgaagag tacaactggg atctgaacta cggcgaaatc
    17221 gcgaagattt tccgtgctgg ctgcatcatc cgtgcgcagt tcctgcagaa aatcaccgat
    17281 gcttatgccg aaaatccgca aatcgctaat ctgctgctgg ctccgtactt caagcaaatt
    17341 gccgatgact accagcaggc gctgcgtgat gtcgttgctt atgcagtaca gaacggtatc
    17401 cctgttccga ccttcgctgc tgcggttgcc tattacgaca gctaccgcgc cgctgttctg
    17461 cctgcgaacc tgatccaggc acagcgtgac tatttcggtg cgcatactta taagcgcatt
    17521 gataaagaag gtgtgttcca taccgaatgg ctggattaat ctgatttaaa tcaattaatc
    17581 aatgcaaggc ccggagaaac cctccgggct tttttattat acaaagcggc atgttatggc
    17641 ctttttttat aatttatagt taaaaacgcg atataataca gcgccgcaca gcaggatcgc
    17701 tgccttgaca gttcatctac atcagcgtta aaaatcccgc agtagatgaa gttgtggtgg
    17761 tggattaatg accactctaa atgtttaacc ggaagaagtc agagctaatg aaaataacaa
    17821 tttcaggaac aggttatgtt ggtctttcaa atggtattct gattgcgcaa aatcacgaag
    17881 tggttgcact ggatatcgtt caggccaaag tggacatgct taacaagaag cagtcaccga
    17941 ttgttgataa ggagattgaa gagtatctgg cgactaaaga tctcaatttc cgcgctacga
    18001 cagataagta tgacgcgtat aaaaatgccg attacgttat tattgccacg cctaccgatt
    18061 atgacccgaa aacaaattat tttaatacct caagcgtgga agcggtcatt cgcgatgtga
    18121 cagaaattaa tcctaatgct gtcatggtga ttaaatctac aattccggta ggtttcacca
    18181 aatcaattaa agaacgtctg ggtatcgata atcttatttt ctctccggaa tttctccgtg
    18241 aaggtaaagc cctttacgat aatctccatc cttcacgtat tgtcattggt gagcgttcag
    18301 aacgcgcaga acgttttgct gcgttgttac aggaaggcgc gattaagcaa aatatcccga
    18361 ccctgtttac cgactccact gaagcagaag cgattaaact tttcgctaat acctatctgg
    18421 cgatgcgcgt agcatacttt aatgaactgg atagctatgc agaaagttta ggtctgaata
    18481 ctcgccagat tatcgaaggc gtttgtctcg atccgcgtat tggcaaccat tacaacaatc
    18541 cgtcgtttgg ttatggtggt tattgtctgc cgaaagatac caagcagtta ctggcgaact
    18601 accagtctgt gccgaataac ctgatttcgg caattgtcga tgcaaaccgt acacgtaaag
    18661 attttattgc cgatgccatt ttgtcacgca agccgcaagt ggtgggtatt tatcgtctga
    18721 ttatgaagag tggttcagat aacttccgcg cgtcttccat tcaggggatt atgaagcgta
    18781 tcaaggcgaa aggcgttgaa gtgatcatat acgagccagt gatgaaagaa gactcattct
    18841 tcaactctcg cctggaacgt gatctcgcca ccttcaaaca acaagccgac gtcattatct
    18901 ccaaccgtat ggcagaagag cttaaggatg tggcagataa ggtatacacc cgcgatctct
    18961 ttggcagcga ctaacatcct gttatcaggg cgattttcgc cctgattctc ttatgttccc
    19021 tttgtaataa ttcattattt ttatcattta tcctatagca ttcatggcga ttatcgctaa
    19081 actatggcga cttaaaatta tttcgtccgt tagggtaatg atgagagtag aaaataataa
    19141 tgtttctggg caaaaccatg acccggaaca gattgatttg attgatttac tggtgcagtt
    19201 gtggcgtggc aagatgacaa ttatcatctc tgtcattgtc gctattgccc tggctatagg
    19261 gtatctggcc gttgccaaag aaaagtggac ttctacagct atcgttactc aacctgatgt
    19321 ggggcaaatc gctggttata acaatgccat aaatgtgatt tatggttcgg cagcgccgaa
    19381 agtgtctgag atccagtcga tgttaattgg tcgtttcagc acgacatttt ctgcattagc
    19441 agaaacgctg gataatcagg aagaacctga aaaactgacc attgagccta ccgttaaaaa
    19501 tcagtcatta cctttagcgg tttcttatgt gggtgaaaca cctgaaggtg cgcagaaaca
    19561 gctggcgcag tatatccagc aagttgacga ccaggtaaat gaagaactgg aacaagacct
    19621 gaaagacaac atcgcgctgc aaatgaaaaa cttgcaggat tctctgaaga cccaggaagt
    19681 ggttgcgcag gagcagaaag atctgcgtat ccgtcagatt caggaagcgt tgcagtatgc
    19741 gaatcaggcg caggtgacaa agccgcagat tcagcagaca caggacgtaa cccaggatac
    19801 tatgttcctt ttggggagcg aagcgctgga gtcgatgatt aagcacgagg cgactcgtcc
    19861 gttggtgttc tcatcaaact actatcagac tcgtcaaaac ctgctggata tcgacaatct
    19921 tgacgttgat aaacttgata ttcatgctta ccgctatgta atgaaaccga cgttacctat
    19981 tcgtcgcgat agcccgaaaa aggcaattac cttgattctg gcggtgctgc tgggctgcat
    20041 ggttggcgcg gggattgtgt tggggcgtaa cgctctgcgt aattacaacg cgaagtaatc
    20101 ttttcggttt taaagaaaat gggcagggtg gtgacacctt gcccgttttt ttgccggatg
    20161 cgacaacaac atcgcatccg cttaccccgc aactcactga tgccgtttac gcaggttctc
    20221 aatcaccgtc gttaagtcca gatcctgatc ctgcaacagc accagcagat gatacatcaa
    20281 gtcagatgcc tcgttggtca gctcaaagcg gtcatgtaca gtcgcggcca gtgcggtttc
    20341 cacgccttct tcgcccactt tctgcgcaat gcgtttggtg ccgctggcat acagtttggc
    20401 agtgtaagag gtttccgggt cggcagattt gcgttcggcg agcagttgtt ccagttgata
    20461 caggaacagc cactggtgag cggtatcgcc gaagcagctg ctggtgcctt tgtggcaggt
    20521 tggcccgatg ggattcgcca gcaccagtaa agtgtcgttg tcgcagtccg gggcaatact
    20581 caccacattg agaaaattgc ccgacgtttc gcctttggtc cacagtcgct gtttagtgcg
    20641 cgagaagaag gtgactttgc cgctttcgat ggttttgtct aaggcttccg ggttcatata
    20701 gcccagcatt agcacttcgc cggataccgc gtgttgcaca atcaccggca tcagtccgtc
    20761 ggttttttcc cagtccagtt cgcgacgttg ttgttctgtt aacatatcct gatctccacg
    20821 ccctgtgttg ccaggtacgc ttttaattca ccaatattga ttatttgttt gtggaatacg
    20881 gaagctgcca gcgcgccgtc aacgtcggca tcacggaagg cttcgaggaa gtgttccatg
    20941 gtccccgcgc caccggaggc aatcagcggg acgtggcaaa cttcacgtac ttttttcagc
    21001 tgctcaaggt cgtaaccgtt acgcacgccg tcctgattca tcatgttgag gacgatttct
    21061 ccggctccac gtttttgcac ttcctgtacc cagtcgagcg tttcccactg agtgacgcgg
    21121 gtgcgggttt catcgccggt atattgattc acatgatatt taccggtttc agcgtcgtac
    21181 caggtatcaa taccgaccac aatacactgc acgccaaagc
//
