LOCUS       AF363060                4513 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Streptococcus agalactiae strain SG99/077 capsular polysaccharide
            biosynthesis gene cluster, partial sequence.
ACCESSION   AF363060
VERSION     AF363060.1
KEYWORDS    .
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Kong,F., Gowan,S., Martin,D., James,G. and Gilbert,G.L.
  TITLE     Serotype identification of group B streptococci by PCR and
            sequencing
  JOURNAL   J. Clin. Microbiol. 40 (1), 216-226 (2002)
   PUBMED   11773119
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Kong,F. and Gilbert,G.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2001) CIDMLS, ICPMR, Westmead Hospital, Darcy
            Road, Sydney, NSW 2145, Australia
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /strain="SG99/077"
                     /serotype="Ia"
                     /db_xref="taxon:1311"
     gene            <1..126
                     /gene="cpsD"
     CDS             <1..126
                     /gene="cpsD"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD"
                     /protein_id="AAL56395.1"
                     /translation="AKEQMEQSGSKFLGIILNKVSESVATYGDYGNYGKRDRKRK"
     gene            352..1527
                     /gene="cpsE"
     CDS             352..1527
                     /gene="cpsE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAL56396.1"
                     /translation="MVLKYSFYYIFISSSLFFIFKNSFTTTRLSFFSFIAMNSILLYL
                     LNSFLKYYRKYSYAKFSRDTKVVLITNKDSLSKMTFRNKYDHNYIAVCILDSSEKDCY
                     DLKHNSLRIINKDALTSELTCLTVDQAFINIPIELFGKYQIQDIINDIEAMGVIVNVN
                     VEALSFDNIGEKRIQTFEGYSVITYSMKFYKYSHLIAKRFLDITGAIIGLLICGIVAI
                     FLVPQIRKDGGPAIFSQNRVGRNGRIFRFYKFRSMRVDAEQIKKDLLVHNQMTGLMFK
                     LDDDPRITKIGKFIRKTSIDELPQFYNVLKGDMSLVGTRPPTVDEYEKYNSTQKRRLS
                     FKPGITGLWQISGRNNITDFDEIVKLDVQYINEWSIWSDIKIILLTLKVVLLGTGAK"
     gene            1551..2000
                     /gene="cpsF"
     CDS             1551..2000
                     /gene="cpsF"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAL56397.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            2000..2482
                     /gene="cpsG"
     CDS             2000..2482
                     /gene="cpsG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAL56398.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMNAVSKGKKTIVVPRQEQFGEHVNN
                     HQVDFLKELFLKYELDYILNISELENIIKEKNISTSKVISQNNDFCSSFKNELSKLFE
                     "
     gene            2578..3717
                     /gene="cpsH"
     CDS             2578..3717
                     /gene="cpsH"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAL56399.1"
                     /translation="MQLLLLLALIVLLICSSYNEKMKFLNMAEIFFIVFYIIYLTSIL
                     LHSLFKNPDFDRILAAFNSLIIGIVSLAFYHYYKNTTLELDKILKAFLFNGLILFFLG
                     GTYYYCLHNNIQNISIFGRDLIGSDWINGMHTQRAMGFFEYSNLIIPITVVTNLYIYI
                     YMKLRNYSIMTIGVVLLFTFILPIGSGSRAGIVAILAQMFILLLNTVVVKKKTIKFLL
                     YILPFLLVIVMMLYFDNLLSIYYRIINLRSGSSESRFSLYKDTVNIVINNSLLFGEGV
                     KELWLNSDLPLGSHSTYIGYFYKSGLLGLMNIVPGLLLIFTNIGRKAKQSAFYYEIVG
                     TLITLFSFFALEDLDGANWLIVFIFTVLGILENKDFYSQLKRWKS"
     gene            3717..>4513
                     /gene="cpsI"
     CDS             3717..>4513
                     /gene="cpsI"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAL56400.1"
                     /translation="MEKRILVSIIIPIYNSEAYLKECVQSVLQQTHPLIEVILIDDGS
                     TDNSGEICDNLSQEDNRILVFHKKNGGVSSARNLGLDKSTGEFITFVDSDDFVAPNMI
                     EIMLKNLITENADIAEVDFDISNERDYRKKKRRNFYKVFKNNNSLKEFLSGNRVENIV
                     CTKLYKKSIIGNLRFDENLKIGEDLLFNCKLLCQEHRIVVDTTSSLYTYRIVKTSAMN
                     QKFNENSLDFITIFNEVSSLVPAKLANYVEAKFLREKIKCLRKMFELG"
BASE COUNT     1574 a    553 c    795 g   1591 t
ORIGIN
        1 gcaaaagaac agatggaaca aagtggttca aagttcttag gtattattct taataaagtt
       61 agtgaatctg ttgctactta cggcgattat ggaaattacg gaaaaaggga tagaaaaagg
      121 aagtaagggg ctcttgtatt gaaagaaaaa gaaaatatac aaaagattat tatagcgatg
      181 attcaaacag ttgtggttta tttttctgca agtttgacat taacattaat tactcccaat
      241 tttaaaagca ataaagattt attgtttgtt ctattgatac attatattgt cttttatctt
      301 tctgattttt acagagactt ttggagtcgt ggctatcttg aagagtttaa aatggtattg
      361 aaatacagct tttactatat tttcatatca agttcattat tttttatttt taaaaactca
      421 tttacaacga cacgactttc ctttttttct tttattgcta tgaattcgat tttattgtat
      481 ctattgaatt catttttaaa atattatcga aaatattctt acgctaagtt ttcacgagat
      541 accaaagttg ttttgataac gaataaggat tctttatcaa aaatgacctt taggaataaa
      601 tacgaccata attatattgc tgtctgcatc ttggactcct ctgaaaagga ttgttatgat
      661 ttgaaacata actcgttaag gataataaac aaagatgctc ttacttcaga gttaacctgc
      721 ttaactgttg atcaagcttt tattaacata cccattgaat tatttggtaa ataccaaata
      781 caagatatta ttaatgacat tgaagcaatg ggagtgattg tcaatgttaa tgtagaggca
      841 cttagctttg ataatatagg agaaaagcga atccaaactt ttgaaggata tagtgttatt
      901 acatattcta tgaaattcta taaatatagt caccttatag caaaacgatt tttggatatc
      961 acgggtgcta ttataggttt gctcatatgt ggcattgtgg caatttttct agttccacaa
     1021 atcagaaaag atggtggacc ggctatcttt tctcaaaata gagtaggtcg taatggtagg
     1081 atttttagat tctataaatt cagatcaatg cgagtagatg cagaacaaat taagaaagat
     1141 ttattagttc acaatcaaat gacagggcta atgtttaagt tagacgatga tcctagaatt
     1201 actaaaatag gaaaatttat tcgaaaaaca agcatagatg agttgcctca attctataat
     1261 gttttaaaag gtgatatgag tttagtagga acacgccctc ccacagttga tgaatatgaa
     1321 aagtataatt caacgcagaa gcgacgcctt agttttaagc caggaatcac tggtttgtgg
     1381 caaatatctg gtagaaataa tatcactgat tttgatgaaa tcgtaaagtt agatgttcaa
     1441 tatatcaatg aatggtctat ttggtcagat attaagatta ttctcctaac actaaaggta
     1501 gtcttacttg ggacaggtgc taagtaaagg taaggtttga aaggaatata atgaaaattt
     1561 gtctggttgg ttcaagtggt ggtcatctag cacacttgaa ccttttgaaa cccatttggg
     1621 aaaaagaaga taggttttgg gtaacctttg ataaagaaga tgctaggagt attctaagag
     1681 aagagattgt atatcattgc ttctttccaa caaaccgtaa tgtcaaaaac ttggtaaaaa
     1741 atactattct agcttttaag gtccttagaa aagaaagacc agatgttatc atatcatctg
     1801 gtgccgctgt agcagtacca ttcttttata ttggtaagtt atttggttgt aagaccgttt
     1861 atatagaggt tttcgacagg atagataaac caactttgac aggaaaatta gtgtatcctg
     1921 taacagataa atttattgtt cagtgggaag aaatgaaaaa agtttatcct aaggcaatta
     1981 atttaggagg aattttttaa tgatttttgt cacagtgggg acacatgaac agcagttcaa
     2041 ccgtcttatt aaagaagttg atagattaaa agggacaggt gctattgatc aagaagtgtt
     2101 cattcaaacg ggttactcag actttgaacc tcagaattgt cagtggtcaa aatttctctc
     2161 atatgatgat atgaactctt acatgaaaga agctgagatt gttatcacac atggcggtcc
     2221 agcgacgttt atgaatgcag tttctaaagg gaaaaaaact attgtggttc ctagacaaga
     2281 acagtttgga gagcatgtga ataatcatca ggtggatttt ttgaaagagt tattcttgaa
     2341 atatgaatta gattatattt tgaatatcag tgaattagag aatattatta aggaaaaaaa
     2401 tatatctact agtaaagtaa tatcacaaaa caatgatttt tgttcctctt tcaaaaatga
     2461 actttctaaa ctatttgaat aaatatattt tgttggagaa aaaaattgaa attaactatc
     2521 aatccaaagt atttgttaat aggaggaatt ttcgctttaa ccctattttc aaagccaatg
     2581 caacttttgt tacttttagc attaatagtt ttacttattt gtagtagtta taatgaaaaa
     2641 atgaaatttt taaatatggc tgaaattttt ttcattgtat tttatattat ttatttaact
     2701 tcaatattgc tacattcttt gtttaaaaat cctgattttg atagaatttt agcagctttt
     2761 aactcgttga ttatcggtat agtatcattg gctttttatc attattataa gaatacaact
     2821 ttggagttag ataaaatatt aaaagcattt ttatttaatg ggttaatcct atttttttta
     2881 gggggaacat attattattg tttgcataat aatattcaaa atatcagtat ttttggtaga
     2941 gatttgattg ggtcagactg gattaatggt atgcatactc aaagagcaat gggatttttt
     3001 gaatattcaa accttataat tcctattaca gtggtaacta acttatatat atatatatat
     3061 atgaagttaa gaaactattc aattatgacc ataggtgttg tattattatt tacctttatt
     3121 ttacctattg gatcgggctc cagggctgga atagtagcta tattggcgca gatgtttatt
     3181 cttcttctaa atacagttgt cgtaaagaag aaaactataa aatttttatt gtacatactt
     3241 ccgtttctac tagtaatagt aatgatgtta tattttgata acttactatc tatatattat
     3301 cgtataatta atttgcgatc cgggagtagt gaatccagat tttctttata taaagataca
     3361 gtaaacatcg ttataaataa ttctttatta tttggagaag gagttaaaga gttatggtta
     3421 aatagtgatc tccctttggg gtcgcattca acgtatatag gctatttcta caaaagtggc
     3481 ctgctgggat taatgaatat agttccaggt ttgcttttaa tttttactaa tattggtagg
     3541 aaagctaaac aatcagcttt ttattatgag atagtaggaa cacttataac tttattctca
     3601 ttttttgcac ttgaagatct tgacggagct aattggctta ttgtttttat ttttacagtg
     3661 ttaggaattt tagaaaataa ggatttttat agtcaactta aaaggtggaa aagttaatgg
     3721 aaaaacgaat acttgtttct atcattatac ctatatacaa ctcagaagca taccttaaag
     3781 aatgtgtgca atccgtacta caacagactc atccattgat agaagttata ctaattgatg
     3841 atggatccac tgataatagt ggagaaattt gtgataattt atctcaagaa gataatcgca
     3901 tacttgtatt tcataaaaaa aatggagggg tctcttcggc aaggaaccta ggtctagata
     3961 aatccacagg agaattcata acatttgtgg atagtgatga ttttgtagca ccgaatatga
     4021 ttgaaataat gttaaaaaat ttaatcactg agaatgctga tatagcagaa gtagattttg
     4081 atatttcgaa tgagagagat tatagaaaga agaaaagacg aaacttttat aaagtcttta
     4141 aaaacaataa ctctttaaaa gaatttttat caggcaatag agtggaaaat attgtttgta
     4201 caaaattata taaaaaaagt ataattggca acttgaggtt tgatgagaac ttaaaaattg
     4261 gtgaggattt actttttaat tgtaaactct tatgtcaaga gcaccgtata gtcgtagata
     4321 cgacttcttc cttatatact tatcgaattg taaaaacttc cgcaatgaat cagaaattca
     4381 acgaaaactc attagatttt ataacaattt ttaatgaagt aagtagtttg gttcctgcca
     4441 aattggctaa ttatgttgaa gcgaaatttt taagagaaaa gataaagtgt ctccgaaaaa
     4501 tgtttgaatt agg
//
