LOCUS       AF337958               16448 bp    DNA     linear   BCT 29-APR-2005
DEFINITION  Streptococcus agalactiae strain NT6 type VI capsular polysaccharide
            synthesis operon, complete sequence.
ACCESSION   AF337958
VERSION     AF337958.1
KEYWORDS    .
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16448)
  AUTHORS   Chaffin,D.O., McKinnon,K. and Rubens,C.E.
  TITLE     CpsK of Streptococcus agalactiae exhibits
            alpha2,3-sialyltransferase activity in Haemophilus ducreyi
  JOURNAL   Mol. Microbiol. 45 (1), 109-122 (2002)
   PUBMED   12100552
REFERENCE   2  (bases 1 to 16448)
  AUTHORS   McKinnon,K., Chaffin,D.O. and Rubens,C.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2001) Infectious Disease, Immunology and
            Rheumatology, Children's Hospital and Regional Medical Center, 4800
            Sand Point Way NE, Seattle, WA 98105, USA
FEATURES             Location/Qualifiers
     source          1..16448
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /strain="NT6"
                     /serotype="VI"
                     /db_xref="taxon:1311"
     gene            895..2425
                     /gene="cpsA"
     gene            895..16444
                     /gene="type VI capsular polysaccharide synthesis operon"
     regulatory      895..900
                     /regulatory_class=minus_35_signal
                     /gene="cpsA"
     regulatory      919..924
                     /regulatory_class=minus_10_signal
                     /gene="cpsA"
     regulatory      956..961
                     /regulatory_class=ribosome_binding_site
                     /gene="cpsA"
     CDS             968..2425
                     /gene="cpsA"
                     /note="similar to LytR"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsA"
                     /protein_id="AAK11658.1"
                     /translation="MSNHSRRHQKKHSHTPLRVINLFLLVIFILLSVVSLFLMYRHHF
                     LAFRHLNVIYGVVIILIILASLFLCIKNKARIFTTIILVLSSIFVATTLYGFKSTIDL
                     TNNLNKTASYSEIEMSVVVPKDSKITNIEAVSKLAAPVKNDTSNITDLIEHIKSEKGI
                     SITPQKTDSYQDAYNRIKNGDSQAMVLNNAYVSLIELSTPDFKSQIKTIYTYKIKKKI
                     NRKNTNHKEGVFNIYISGIDTFGSISTVSRSDVNIIMTVNTNTHKVLLTTTPRDAYVK
                     IPDGGDNQYDKLTHAGLYGVETSMKTLENLYDINLDYYARINFSSFLKLIDLLGGVTV
                     YNDQAFTSKHGNFDFPVGQVTLNSEQALGFVRERYSLQGGDNDRGRNQEKVIAAIINK
                     LASSQSVTKLNSITSQLQTSVQTNMTIDNINDLINNQLSTGQRFTVESQALTGHGSTG
                     ELPSYAMPGAQLYMMSIDQSSLSNAKSKIKNTMEE"
     gene            2415..3162
                     /gene="cpsB"
     regulatory      2415..2421
                     /regulatory_class=ribosome_binding_site
                     /gene="cpsB"
     CDS             2431..3162
                     /gene="cpsB"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phosphatase CpsB"
                     /protein_id="AAK11659.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKERVKEIINMGCYT
                     QINSSHILKQKLFNDKHKRFKKRARYFLEENLVHFVASDMHNLDVRPPFLAEAYKIIC
                     RDFGKERANQLFIENAQSILKNHYI"
     gene            3161..3863
                     /gene="cpsC"
     regulatory      3161..3167
                     /regulatory_class=ribosome_binding_site
                     /gene="cpsC"
     CDS             3171..3863
                     /gene="cpsC"
                     /codon_start=1
                     /transl_table=11
                     /product="putative chain length regulator CpsC"
                     /protein_id="AAK11660.1"
                     /translation="MNKIANTEVEINIFNLLKKLWKKKFLITFVAIAFATAGLFYSLF
                     IVTPQYTSSTRIYVINPNTPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKL
                     NYPSSQLLQKITVSILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDI
                     TTLEKGNLPKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGL
                     TSLGIVPDLNKL"
     gene            3863..4563
                     /gene="cpsD"
     regulatory      3863..3868
                     /regulatory_class=ribosome_binding_site
                     /gene="cpsD"
     CDS             3874..4563
                     /gene="cpsD"
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide chain length
                     regulator/exporter CpsD"
                     /protein_id="AAK11661.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAITSVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIASACDGFVLVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVNESVATYGDY
                     GNYGKRDRKRK"
     gene            4577..5964
                     /gene="cpsE"
     regulatory      4577..4582
                     /regulatory_class=ribosome_binding_site
                     /gene="cpsE"
     CDS             4615..5964
                     /gene="cpsE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAK11662.1"
                     /translation="MIQTVVVYVSVSLTLTLITPNFKSNKDLLFVLLIHYIVFYLSDF
                     YRDFWSRGYLEEFKMVLKYSFYYIFISSSLFFIFKNSFTTTRLSFFTFIAMNSILLYL
                     LNSFLKYYRKYSYAKFSRDTKVVLITNKDSLSKMTFRNKYDHNYIAVCILDSSEKDCY
                     DLKHNSLRIINKDALTSELTCLTVDQAFINIPIELFGKYQIQDIIIDIEAMGVIVNVN
                     VEALSFDNIGEKRIQTFEGYSVITYSMKFYKYSHLIAKRFLDIMGAIIGLLICGIVAI
                     FLVPQIRKDGGPAIFSQNRVGRNGRIFRFYKFRSMRVDAEQIKKDLLVHNQMTGLMFK
                     LEDDPRITKIGKFIRKTSIDELPQFYNVLKGDMSLVGTRPPTVDEYEKYNSTQKRRLS
                     FKPGITGLWQISGRNNITDFDEIVKLDVQYINEWSIWSDIKIILITLKVVLLGTGAK"
     gene            5975..6437
                     /gene="cpsF"
     regulatory      5975..5981
                     /regulatory_class=ribosome_binding_site
                     /gene="cpsF"
     CDS             5988..6437
                     /gene="cpsF"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAK11663.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKIYPKAINLGGIF"
     gene            6422..6910
                     /gene="cpsG"
     regulatory      6422..6428
                     /regulatory_class=ribosome_binding_site
                     /gene="cpsF"
     CDS             6437..6910
                     /gene="cpsG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAK11664.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMSVISLGKLPVVVPRRKQFGEHIND
                     HQIQFLNSIAHLYPLAWIEDVDGLAEALKRNIATEKYQGNNDMFCHKLEKIIGEI"
     gene            6898..8064
                     /gene="cpsH"
     regulatory      6898..6903
                     /regulatory_class=ribosome_binding_site
                     /gene="cpsG"
     CDS             6907..8064
                     /gene="cpsH"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAK11665.1"
                     /translation="MRKYLDLDYSLFYALWVLILVPNQWYQFLIITIIVLLLLWKSEF
                     RISISNSSILFLLWLFIYLFAILIRGTQEDITFQRFIAELLKLISTGYALFFYNYYRK
                     ADFNSSVVRNVVKVNYFVLFLITVLYLFFPNEFTTFLGRDLFSIEWIPSMKVRLTAYF
                     EYATLLGQFILFTYPILFLKQQRYGENIFITLFLVFCAYLTGARIFLICMIILLGYLL
                     LEIIINKFNLKITKKAVFLIIIGIILLLVCFSYKVESIINYIIHYRFQSSSTRLTVYY
                     ESIRAILDGNFLIGQGIRVPSSVGIFLGSHSSYISIFYRTSFTGLFLFFSILLFLYRE
                     AIKQNRIIYKLFFGLLLLYMVFEEFDPNHWSVVLLFTTLGIVGRGNDKKTS"
     gene            8034..9013
                     /gene="cpsVII"
     regulatory      8034..8040
                     /regulatory_class=ribosome_binding_site
                     /gene="cpsVII"
     CDS             8045..9013
                     /gene="cpsVII"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAK11666.1"
                     /translation="MIKKLVSVIVPVYNSELVIENCVESLLQQTYPEIEILLIDDGST
                     DKSSHICNNFLKRDSRVKVYHKYNGGASSARNVGLEMAEGEFITFVDSDDVVALNMIE
                     IMLNNLLTENADISEIDFEVSDDFYKRKKRKGYYRVFQNNKSLKEFFSGNKVENVVWG
                     KLYKKSIIGDLRFNEKYKIGEDLLFNFQILNKEHRIVVDTRRSLYTYRIEEKSIMNQQ
                     FNKNTLDFIDIFNEIHQDSPTELFNYVEAKFVREKIKCLRKMFELGEIADENLRLQRY
                     KFWQDIKSYSICKAIRFLSKKHICTLYLMKYFPYVYIKMYNKFQKQ"
     gene            9031..9752
                     /gene="cpsVIJ"
     regulatory      9031..9037
                     /regulatory_class=ribosome_binding_site
                     /gene="cpsVIJ"
     CDS             9060..9752
                     /gene="cpsVIJ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAK11667.1"
                     /translation="MSIYNEPLKYIKSSIDSILSQTLKNFEFIIVIDNPDREDVYDIL
                     QTYLASDARVKVLSNERNMGLAYSLNKAIKYSVGNYIFRMDADDISYPERFEKQINFM
                     EKYRLDFSATLIEVMDEDEETLYKQKNINKNYFSEDIYRMLSNRCVLSHPTWCVKRAV
                     FLRLDGYRNIVPAEDYDFLLRLALSGIKCGLIGDILLRYRSNANGISQLININSIYTQ
                     LYFRIGSKMDKC"
     gene            9965..10913
                     /gene="cpsK"
     regulatory      9965..9971
                     /regulatory_class=ribosome_binding_site
                     /gene="cpsK"
     CDS             9978..10913
                     /gene="cpsK"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAK11668.1"
                     /translation="MMSNIYVCHTLYHLLICLYKETDYTNTVIVLSSSILNASELQKK
                     LKSVGANVYILTESAKENQGVILKLKKIGLRNTNGRSNCFIFNDATPIGRTLIKYGIY
                     YNLIEDGLNCFTYSIFRQRLWKYYVKKYILHKVQPYGFSRYCLGIEVNSLVGLPKDPR
                     YKKFIEVPRKELFDNATEYQKEMAINLFGAVKVSINSPSVLILTQPLSKDKEFMSYNN
                     KIETSEEQFNFYKSIVDEYINKGYNVYLKVHPRDVVDYSKLPVELLPSNVPMEIIELM
                     LTGRFECGITHSSTALDFLTCVDKKITLVDLKDIK"
     gene            10897..12310
                     /gene="cpsL"
     regulatory      10897..10903
                     /regulatory_class=ribosome_binding_site
                     /gene="cpsK"
     CDS             10910..12310
                     /gene="cpsL"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAK11669.1"
                     /translation="MNTKKLLQSGFIYTIGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMNIIMLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIILLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYANYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVFGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSVPLIF
                     HLLGHQLLNQLDRIMLARLYSTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EKLLQLSTYISRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALLI
                     FHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLDNIVIRWSLGIIILIVYSIV
                     FQKVILDLLSKKRRRR"
     gene            12299..13329
                     /gene="neuB"
     regulatory      12299..12305
                     /regulatory_class=ribosome_binding_site
                     /gene="neuB"
     CDS             12310..13329
                     /gene="neuB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative N-acetyl neuramic acid synthetase NeuB"
                     /protein_id="AAK11670.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAIGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLP"
     gene            13400..14566
                     /gene="neuC"
     regulatory      13400..13406
                     /regulatory_class=ribosome_binding_site
                     /gene="neuC"
     CDS             13412..14566
                     /gene="neuC"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-N-acetylglucosamine-2-epimerase
                     NeuC"
                     /protein_id="AAK11671.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPPLTKEAIEKQLGIALEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLTNHENSHCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETSSVLEG
                     LSKLNEVTNFDNPYYKENASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            14547..15192
                     /gene="neuD"
     regulatory      14547..14552
                     /regulatory_class=ribosome_binding_site
                     /gene="neuD"
     CDS             14563..15192
                     /gene="neuD"
                     /note="similar to Escherichia coli K1 NeuE"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD"
                     /protein_id="AAK11672.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            15120..16444
                     /gene="neuA"
     regulatory      15120..15125
                     /regulatory_class=ribosome_binding_site
                     /gene="neuD"
     CDS             15203..16444
                     /gene="neuA"
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N-acetylneuraminic acid synthetase NeuA"
                     /protein_id="AAK11673.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPSLFTTLSDKGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSRTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISTSLESYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGYSVEEIFSNFQKLYSLLAENKIKMRLTT
                     IAYTLFRETVNNADIEKINQWLTEFCYQNQIPLLDINRFLSKDGNLNYHLTSDGLHFT
                     QEANDLLQSQYQLFVDEVKTL"
BASE COUNT     5676 a   2271 c   2903 g   5598 t
ORIGIN
        1 tttcgtcacc gttcaatttt gagttgagga taccagttgc cactgtataa ccatctaatc
       61 caatcattaa attaaataga gttgctctat cactgacaac aatggatttt gggtgaggaa
      121 tttgagacat catttcctca gaaaagtaga aagaattatg caggccttga tcataactca
      181 gataaggata atcttctaaa tctttcatac ttaatttttt tctattagct aagggattgg
      241 atttgctcac gaaaatatga ggtgtagtag taaaaagagt tgtggcaatt aaggaattat
      301 cgtcgaataa tttggttaat acatctctat tatagctatt aagaaacaga acaccaatct
      361 ctgaacggaa atttttaaca tcatcaataa tttcccaagt cctagtttca cgtaaaaaaa
      421 gttcgtattg ggtcatgtct gtgccattaa agagagctac aaaagcgtta accacaaatg
      481 catagtgttg tgaagaaacg ctgaacagct cacgacttgt attatcaccc ttataacgct
      541 cttcaagaag tgctgtttgt tctagaattt gtcgcgcata tgacagaaat tccataccat
      601 cttttgttaa cgttattcct tttggatttc gaataaaaat ttggataccc atctcagtct
      661 caaggttcct aacagcgttt gaaagtgaag gttgggtaat atagagttgt ttagcggctt
      721 cattcatgct accagtttct acaatcttaa taacatattg taattgttga attctcataa
      781 ctctagttta aactagaatg taaataaatt caattagatc agctaaatta tcaatataga
      841 tcaattgaaa atgttattta gctaatataa tcagattatt ccaatattac tttcgttgaa
      901 tattgtattg aaaaatgata taatatccaa gtaaaattaa ggttttttta agtattggag
      961 aattacaatg tctaatcatt cgcgccgtca ccaaaagaaa cactcacata cacctctacg
     1021 ggtgattaat ttatttcttt tggtgatttt tattttgtta agtgtagtct cattatttct
     1081 tatgtatcgt caccattttt tggcatttag acacttgaac gtcatttatg gagttgtaat
     1141 tattttaatc attttagcaa gtttatttct ttgtattaag aataaagcta gaatttttac
     1201 aactataatt ttagtactat cctctatttt cgttgctact actttatatg gatttaagtc
     1261 aaccattgat ttgacaaata atctaaataa aactgcttca tactctgaaa ttgagatgag
     1321 tgtagttgta ccaaaagatt ctaaaataac caatatagaa gctgtcagca aattagccgc
     1381 accagttaaa aacgatactt caaatattac tgatttgata gaacatataa aatcagaaaa
     1441 aggaatctct attacaccac aaaaaacaga ttcttaccag gatgcataca atagaattaa
     1501 aaatggtgat agtcaagcta tggttttaaa taatgcttat gttagcttaa ttgaacttag
     1561 cacccctgat tttaaatcgc agataaaaac gatttatact tacaaaatta agaaaaaaat
     1621 taatcgtaaa aatactaatc ataaagaagg ggtatttaat atctatatta gcggtattga
     1681 tacttttggc tctatatcaa cagtatcaag atctgatgta aatattatta tgacggttaa
     1741 taccaatacc cacaaagtat tgttaacgac aacaccacga gatgcctatg taaaaattcc
     1801 agatggtggg gacaatcaat atgataaatt aacccatgca ggtttgtatg gcgttgagac
     1861 atcaatgaaa acacttgaaa acctttacga catcaacctt gattattatg ctagaattaa
     1921 tttttcatca tttttaaaat taatagacct cttgggagga gtgacagttt ataacgatca
     1981 agcttttaca agtaaacatg gtaattttga ctttcctgtt ggtcaagtaa cattgaattc
     2041 tgagcaggct ttgggctttg ttagagaacg ttattctcta caaggaggcg ataacgatag
     2101 aggtagaaat caagaaaaag tgattgcagc tattataaat aagttagctt ctagtcagtc
     2161 agtaacaaaa ttaaatagca ttacctcaca gctccaaacg tccgttcaaa ctaatatgac
     2221 tattgataat attaatgatt tgattaacaa tcaattgtca actggacaac gcttcactgt
     2281 cgagtcacaa gcattaactg gtcatggttc aacgggtgaa ctcccttcat atgcaatgcc
     2341 aggagctcaa ctttatatga tgtcaattga tcaatctagc ttatctaatg caaaatcaaa
     2401 aattaagaac acaatggagg aataacaaga atgattgata ttcattctca tatcgttttt
     2461 gatgtagatg atgggcccaa aacgcttgaa gagagtttat ctttaataga agaaagttat
     2521 cgacaaggtg ttagaataat tgtttcgaca tcacatagaa gaaaaggtat gtttgaaaca
     2581 cctgaagata ttatcttcaa aaacttttct atagttaaac acgaagcgga aaaaagattt
     2641 gaacatcttc aaatcttata tggtggagaa ttgtactata caagtgatat gttagaaaaa
     2701 ctgaagttaa agcaaattcc aactttaaac aatacgaaat ttgctttaat tgaattttct
     2761 atgcaaactt cttggaaaga tattcataca gctttgtcaa atgttttaat gcttggtatt
     2821 acaccagtcg ttgcgcatat agagaggtat aacgctttag agaatcaaaa agaacgggtg
     2881 aaggaaatta ttaatatggg gtgttacaca caaataaata gttcccatat tttgaaacaa
     2941 aaacttttta atgataagca taaacgcttt aagaaaagag cccgttattt tttagaggaa
     3001 aatttagtgc attttgtagc gagtgatatg cataaccttg atgttagacc gccattttta
     3061 gcagaagctt ataagattat ctgtagagat ttcggtaaag aacgtgctaa ccaacttttt
     3121 attgaaaatg ctcaatctat attaaaaaac cattacattt aggagatttc atgaataaaa
     3181 tagctaatac agaagttgaa ataaatatat tcaacctttt aaaaaaattg tggaaaaaga
     3241 aatttttaat cacatttgtt gccattgcat ttgcaacagc tggacttttc tatagtcttt
     3301 ttattgttac gccacaatat acttcttcaa caaggatata tgtcattaac cctaataccc
     3361 ctaataatag tattactgca caagatctac aagcggggag ttttcttgca aatgactata
     3421 aggagattat tacgtctact gacgttctag aaaaagttat ttcttctgaa aaattgaatt
     3481 atccttcgtc tcagttgcta caaaaaataa cagtttctat tttaaaagat acacgtgtta
     3541 tttcaatatc ggtcgaagat gctaatccaa aaatgtctca aaaattagca aattcagtta
     3601 gagaagcagc agtttcaaaa atcaaggcag ttactcaagt agaagatatc actactcttg
     3661 agaagggaaa tttacctaaa gcaccatctt ctcctaatat taaaaagaat gtactaatcg
     3721 ggtttattgt tggtgcagga ttatcgacta ttgttttagt tattatgggt attttggatg
     3781 atcgtgttaa tactgaagaa gatattgaaa aggtcctagg acttacttct ttaggtatag
     3841 taccagattt gaataaactt taaggagaat ataatgactc gtttagaaat agttgatagc
     3901 aagttaagac aagcaaaaaa aacagaagaa tacttcaatg cgatccgtac aaacatacag
     3961 tttagtggaa aggaaaataa aattcttgca attacctctg ttagggaagg ggaaggaaaa
     4021 tccactactt caacaagttt agctttatct ttagctcaag caggatttaa aacattatta
     4081 attgatgcgg atactaggaa ctctgttatg tctggaacct ttaaagcaac tggaactatt
     4141 aaaggcttga cgaattattt atcaggtaat gcagatcttg gagatattat ctgtgaaacc
     4201 aatgttccta gactgatggt cgttccttca gggaaagtac caccaaatcc aacagcatta
     4261 cttcagaacg cttattttaa taagatgatt gaagctatta aaaatatatt tgattatatt
     4321 atcatcgata ctccacctat tggtttagtt gttgatgccg caataatcgc tagtgcttgt
     4381 gatggctttg ttttagtaac ccaagcaggt agaataaaac gtaattatgt tgaaaaagca
     4441 aaagaacaga tggaacaaag tggttcaaag ttcttaggta ttattcttaa taaagttaat
     4501 gaatctgttg ctacttacgg cgattatgga aattacggaa aaagggatag aaaaaggaag
     4561 taaggggctc ttgtattgaa agaaaaagaa aatatacaaa agattattat agcgatgatt
     4621 caaacagttg tggtttatgt ttctgtaagt ttgacattaa cattaatcac tcccaatttt
     4681 aaaagcaata aagatttatt gtttgttcta ttgatacatt atattgtctt ttatctttct
     4741 gatttttaca gagacttttg gagtcgtggc tatcttgaag agtttaaaat ggtattgaaa
     4801 tacagctttt actatatttt catatcaagt tcattatttt ttatttttaa aaactctttt
     4861 acaacgacac gactttcctt ttttactttt attgctatga attcgatttt attatatcta
     4921 ttgaattcat ttttaaaata ttatcgaaaa tattcttacg ctaagttttc acgagatacc
     4981 aaagttgttt tgataacgaa taaggattct ttatcaaaaa tgacctttag gaacaaatac
     5041 gaccataatt atatcgctgt ctgtatcttg gactcctctg aaaaggattg ttatgatttg
     5101 aaacataact cgttaaggat aataaacaaa gatgctctta cttcagagtt aacctgctta
     5161 actgttgatc aagcttttat taacataccc attgaattat ttggtaaata ccaaatacaa
     5221 gatattatta ttgacattga agcaatggga gtgattgtca atgttaatgt agaggcactt
     5281 agctttgata atataggaga aaagcgaatc caaacttttg aaggatatag tgttattaca
     5341 tattctatga aattctataa atatagtcac cttatagcaa aacgattttt ggatatcatg
     5401 ggtgctatta taggtttgct catatgtggc attgtggcaa tttttctagt tccgcaaatc
     5461 agaaaagatg gcggaccggc tatcttttct caaaatagag taggtcgtaa tggtaggatt
     5521 tttagattct ataaattcag atcaatgcga gtagatgcag aacaaattaa gaaagattta
     5581 ttagttcaca atcaaatgac agggctaatg tttaagttag aagatgatcc tagaattact
     5641 aaaataggaa aatttattcg aaaaacaagc atagatgaat tgcctcaatt ctataatgtt
     5701 ttaaaaggtg atatgagttt agtaggaaca cgccctccca cagttgatga atatgaaaag
     5761 tataattcaa cgcagaagcg acgccttagt tttaagccag gaatcactgg tttgtggcaa
     5821 atatctggta gaaataatat tactgatttt gatgaaatcg taaagttaga tgttcaatat
     5881 atcaatgaat ggtctatttg gtcagatatt aagattattc tcataacact aaaggtagtt
     5941 ttactcggga caggagctaa gtaaaggtaa ggtttgaaag gaatataatg aaaatttgtc
     6001 tggttggttc aagtggtggt cacctagcac acttgaacct tttgaaaccc atttgggaaa
     6061 aagaagatag gttttgggta acctttgata aagaagatgc taggagtatt ctaagagaag
     6121 agattgtata tcattgcttc tttccaacaa accgtaatgt caaaaacttg gtaaaaaata
     6181 ctattctagc ttttaaggtc cttagaaaag aaagaccaga tgttatcata tcatctggtg
     6241 ccgctgtagc agtaccattc ttttatattg gtaagttatt tggttgtaag accgtttata
     6301 tagaggtttt cgacaggata gataaaccaa ctttgacagg aaaattagtg tatcctgtaa
     6361 cagataaatt tattgttcag tgggaagaaa tgaaaaaaat ttatcctaag gcaattaatt
     6421 taggaggaat tttttaatga tttttgtcac agtggggaca catgaacagc agttcaaccg
     6481 tcttattaaa gaagttgata gattaaaagg gacaggtgct attgatcaag aagtgttcat
     6541 tcaaacgggt tactcagact ttgaacctca gaattgtcag tggtcaaaat ttctctcata
     6601 tgatgatatg aactcttaca tgaaagaagc tgagattgtt atcacacatg gcggcccagc
     6661 gacgtttatg tcagttattt ctttagggaa attaccagtt gttgttccca ggagaaagca
     6721 gtttggtgaa catatcaatg atcatcaaat acaattttta aattcgattg cccacctgta
     6781 tcccttggct tggattgaag atgtagatgg acttgcggaa gcgttgaaaa ggaatatagc
     6841 tacagaaaaa tatcagggaa ataatgatat gttttgtcat aaattagaaa aaattatagg
     6901 tgaaatatga ggaaatatct agatttagat tattctttat tttatgctct ttgggtactt
     6961 attttagtac caaaccaatg gtatcagttt ttaattatta ccattatagt tctattatta
     7021 ctttggaaga gtgagtttag aatatctata agcaattctt caatactatt tctgctttgg
     7081 ttatttattt atttatttgc aatactcatt agaggtactc aagaggatat aacgtttcag
     7141 cgatttattg ctgagctatt aaaactaatt agtacaggat atgctttatt tttttataat
     7201 tattatagaa aagctgattt taatagttca gttgtaagga atgtggtaaa ggttaactat
     7261 tttgtgttgt ttcttataac agttttatat ttattttttc caaatgaatt tactacattc
     7321 ctaggaagag atttattttc aattgaatgg attccttcta tgaaagttag acttactgca
     7381 tattttgagt atgcaacact attaggtcag tttattttat tcacttatcc gatattattt
     7441 ttaaaacagc agaggtatgg agaaaatatt tttatcacac tattcctagt tttttgtgca
     7501 tatttgacag gggcaagaat tttcctaatt tgtatgataa ttttattagg ttatttactc
     7561 ttagaaataa tcattaataa atttaaccta aaaattacta aaaaagctgt ctttttgata
     7621 attataggga taatattatt attggtatgt ttttcttaca aagtggagtc tattatcaat
     7681 tatataatac actatagatt tcaaagtagt agtacaagat tgacagtcta ttacgaaagt
     7741 ataagagcga ttttagatgg gaatttcctt attgggcaag gtataagagt tccctccagt
     7801 gtgggaatat ttttaggttc acattcatca tacattagta tattttatag aacttctttt
     7861 acggggctgt ttcttttctt ttcaatatta ctttttctat atagagaagc tatcaaacaa
     7921 aacaggataa tctacaagct tttttttgga ttgttattat tgtatatggt atttgaagaa
     7981 tttgatccta atcattggag tgttgtattg ttatttacta cattaggtat agtagggaga
     8041 gggaatgata aaaaaactag ttagtgtgat tgttccagtt tataattcgg agttagtgat
     8101 tgagaactgt gtagaatctt tgcttcaaca aacataccca gaaatagaaa ttttattaat
     8161 agatgatgga tctacagata aaagtagtca tatttgtaat aattttttaa aaagggatag
     8221 tcgcgtaaaa gtctatcata aatacaatgg aggtgcatca tcagcaagaa atgtgggact
     8281 tgagatggca gaaggtgaat ttataacttt tgtagatagc gatgatgttg tcgcactaaa
     8341 tatgattgaa attatgctga ataatttgtt aacggagaac gcagatatat cagaaattga
     8401 tttcgaagtt tcagatgatt tttataaaag aaaaaaaaga aaaggttact atagagtttt
     8461 tcaaaacaat aagtctctca aagaattttt ttcaggaaat aaagtagaaa atgttgtttg
     8521 ggggaaatta tataaaaaaa gcattattgg ggatttacga tttaatgaaa aatacaaaat
     8581 tggtgaagac ttgctattta actttcagat tttaaataaa gaacatcgta tagttgtaga
     8641 tactagaaga tcactctata cttatcgtat tgaagaaaaa tctataatga atcaacaatt
     8701 taataaaaat acattagact tcattgatat ttttaatgag attcatcagg atagtccgac
     8761 agaattgttt aattatgtgg aagcgaagtt tgtacgagaa aaaatcaagt gtttaaggaa
     8821 aatgtttgaa ttaggagaaa tagctgatga aaatttacgt ttacagagat ataaattttg
     8881 gcaagatatt aaatcatatt caatatgcaa agcaataagg ttcttatcta aaaaacatat
     8941 ctgtacgtta tatttgatga aatattttcc gtacgtatat ataaagatgt ataataaatt
     9001 tcaaaagcaa taactaagtc atacaatact aggaggataa tttgaagtat agtgtcatca
     9061 tgtcaattta taatgaacca ttgaagtaca ttaaatcttc aatagattca attttatcac
     9121 aaacattaaa aaactttgaa tttattattg tgattgataa ccctgacaga gaagatgtat
     9181 atgatatttt gcaaacatat ctagcaagcg atgcaagagt aaaagtatta tcaaatgaga
     9241 gaaatatggg attagcttat agtttaaaca aagctataaa atattcagtt ggaaattata
     9301 tttttagaat ggatgcggac gatatttcat atccagaaag atttgaaaag caaattaact
     9361 ttatggaaaa gtatcgttta gacttctcag caactttaat agaagttatg gatgaagatg
     9421 aagaaacatt atataaacag aaaaatataa ataaaaatta tttttcagaa gatatttata
     9481 gaatgctctc taatagatgt gtcctttcac atcccacttg gtgtgttaaa agagcagtat
     9541 ttttaaggct agatggatat agaaacattg ttcctgcaga ggattatgat tttttactaa
     9601 ggttagcgtt gtcaggaatt aaatgtggtt taatcggtga tatattgctt agatatagga
     9661 gtaatgctaa tggtatttca caactaatca atataaacag tatatatacg cagctatact
     9721 tcaggattgg ttcaaaaatg gacaaatgtt aaatgtagac tatattccta tttatcttga
     9781 aagctatata aatagcaata aattttgtca aaaacactta aaaaaatacc tagactttaa
     9841 atttagtagt agtcctattg ctaaaaaagt cttttgggga ggatttttat ttttcaaata
     9901 tccttttgca cgtcgattaa taattcaaga ttttaatatt tacaaaataa aaatattgaa
     9961 cggtaagagg acaacagatg atgagtaata tatatgtgtg tcatacttta taccatttat
    10021 taatttgttt atataaagaa acagattaca cgaatactgt aattgtatta tcttcttcca
    10081 ttttgaatgc tagtgaatta cagaaaaagt taaaaagtgt aggggctaac gtttatattt
    10141 tgacagagtc agctaaggaa aatcaaggag ttatattaaa acttaaaaaa attggtttaa
    10201 ggaacacaaa cggcaggagt aattgtttta tttttaatga tgcaacacct attgggagga
    10261 ctctaataaa gtatggtatt tattataatc taattgaaga tggtttaaat tgctttactt
    10321 actctatatt taggcaaaga ctttggaagt actatgtaaa aaaatatatt cttcacaaag
    10381 ttcagccata tggattttca cgatactgtt tagggattga agttaattca ttagttggtt
    10441 tgccaaaaga tccacgttat aaaaaattta ttgaagtccc taggaaggaa ctttttgata
    10501 atgcaacaga atatcaaaaa gaaatggcaa taaatctttt tggagcagta aaagttagta
    10561 ttaattcacc ttcagtactg atattaacgc agcctttatc taaagataaa gaatttatga
    10621 gttataacaa taagatagaa acgtccgaag aacaatttaa tttttataaa tcaatagtcg
    10681 acgaatatat aaataaagga tacaatgttt atttaaaagt tcatcctaga gatgtagtag
    10741 attattccaa attgccggta gagctattac catcaaatgt tcctatggaa attatagagt
    10801 tgatgttaac aggtcggttc gaatgtggga taacacattc gtctactgcg ctggattttt
    10861 taacttgtgt tgataaaaaa ataactttag tagatctgaa ggatattaaa tgaatactaa
    10921 gaaattactt caatctggtt ttatttatac aatcgggaat ttgttggtgc aaggattagc
    10981 ctttatcaca cttcccatct acactcgggt catctcaaca gaagtttatg gccaatatag
    11041 tttatatgtc gcttggatga atattattat gctttttatt ggccttcaga cgagcggttc
    11101 tttaagttca gcccgggtca aatacggaga ggaatttaaa agctattctg ggagtgcctt
    11161 ttctgtaggt aatatatggt ttcttattat acttttgata gcttttctat ttagaagttt
    11221 tcttgcacca ttagttggtt tttctgaatc tattttttta ttaatggtgt gtcaaagtta
    11281 cgctaactat gtggtgactt tctttggtca gtattttata caacaacaga ggagcttggc
    11341 taatttaata ctatccttag ccaatgcagt ttcatctgtt gcactatctc tatttttaat
    11401 ttttcattgg tccgatgact ttttatctag ggtttttgga gcttttgttc ccactataat
    11461 aactggaata gttgcctttg cttatattta ttatcatagt aaatcttttt acaatcctaa
    11521 gtattttcgg ttcattgtca ctgtgtctgt tcctttgatt tttcatttgt taggacatca
    11581 gttattaaat caattagata ggattatgct agctcgctta tatagtacta aagaagttgc
    11641 aatgtatagt ttcggttatt cgcttggaat gattattcag attgttttga atagtattaa
    11701 tatggcttgg attccatggt tttttgatgc tagaaaagaa aaattactgc aactttcaac
    11761 ttatattagc cgttatcttt atttaggagt tttcttaaca ttaggttatt tgactgtttt
    11821 tcctgagtta gctcaaataa tgggaggaga caagtatagt agtagtgttc aatttatttc
    11881 tttaattatt gtcagctatt ttttagtctt tctctataca tttcctgtaa atatccaatt
    11941 cttctatgct aatacgacat ggattcctat tgggacatta ttagctgctg gtgtaaattg
    12001 gttactaaac cttgtattaa ttcctcatta tgcagcctat ggcgctgcaa tggctacaat
    12061 aatatcttat ttagccttgt tgatttttca tcatattgtt tcgaaagtta aataccatta
    12121 ttcagatgtc tcagttaggc agtacatcat tttaagtggt atagtattta gttatgctat
    12181 gttaatgaac atgttcctag ataatatcgt tattagatgg agtttaggaa taattattct
    12241 tatagtatat agcattgttt ttcaaaaagt cattttagac ttattgagta aaaaaagaag
    12301 gagaagataa tggtttatat tattgcagag attggttgca atcataatgg agatattaat
    12361 cttgcgaaaa aaatggtaga tgttgccgtg tcttgtggtg ttgatgctgt taaatttcag
    12421 acttttaaag ctgagaaact tatttctaaa tttgctccca aagctgaata tcaaaaagca
    12481 actacaggaa cagcagacag tcaacttgag atgacgaaac gtttagagtt aagctttgaa
    12541 gaatacttag aaatgcgtga ttatgcaatt tcaaaaggtg tggagacctt ttcaacacct
    12601 tttgatgaag agtcattaga gttcttaatt tctacagata tgccaattta caaaattcca
    12661 tcaggagaaa tcactaattt accttactta gaaaagattg gcaagcaaca aaagaaagtt
    12721 attctttcga cgggtatggc ggtaatggaa gagatccatc aagcggtgaa tattttacgt
    12781 cagaatggta caaccgacat ttctatttta cattgtacaa cagagtaccc aacaccttac
    12841 ccctctctaa atttaaacgt tattcatact ttgaaagatg aatttaaaga tttaacgata
    12901 ggttattcgg atcattcaat tggatcagaa gtacctatcg cagcagcagc aataggtgca
    12961 gaagttattg aaaaacactt tactttagat actaatatgg aaggtccgga tcataaagcc
    13021 agtgcaacac ctgatatttt agctgcttta gttaaagggg ttcgcattgt tgaacaagcc
    13081 ttaggtagat ttgaaaaaat cccagatcca gtagaagaaa aaaataagat tgttgctcgt
    13141 aaatcagtag ttgctttaaa accaattaaa aaaggcgata tttattcaat agaaaatatt
    13201 acggtgaagc gcccaggtaa tggtatttct cctatgaact ggtatgatat cttgggacaa
    13261 gaagcgcaag atgatttcga agaggatgaa gttattcgtg attcacgctt tgaaaatcaa
    13321 ttgccctagt tataataaca cctaattata atcggcaaca gatgtattac acggctaaga
    13381 ttatgtaatt actatatata gaagggatac gatgaaaaaa atttgtttag taaccggatc
    13441 gcgtgctgaa tacggaataa tgaagccatt gattcaaaga ttatcaaaag ataaagaagt
    13501 caacttacaa attattgcaa cagcaatgca tctggaagaa aagtacggct atacttatcg
    13561 tcaaattgaa gaagacggtt ttgatattgc ttataaagtt cccttacatc tttatgatac
    13621 tgacagaaga actgtatcta ctgcaatggc gcatttacaa ctaggattga ccaaaatttt
    13681 tgacaaggaa gactatgatc tagtcatcat tttaggggat cgttatgaaa tgttaccagt
    13741 tgtgaatgta gcgttgattt ataatgtccc agtatgccac cttcatggag gggagacatc
    13801 attaggcaat tttgatgagt atattcgcca tgcaattact aagatgagtc acctacactt
    13861 agtctctaca gaggattttc gtcaacgtgt gattcagatg ggagaacaac ctcaatttgt
    13921 aattaacaca ggagctctcg gagtggaaaa tgctctatca attcctcctc taaccaaaga
    13981 agcaatagaa aagcaattag gtattgcact agaggaatcc tattttgttg ttctctatca
    14041 tcctgttact tttgaacaag gaaaaagtgc tggagaacag atgaaagcgg tactatcagc
    14101 cctttctaag tttggtgtcc agtgtctatt tataggttcc aattcagata ctggttctga
    14161 tgatattgca aaggctatca atacttattt gacaaatcat gaaaatagtc attgctttgc
    14221 ttctttatca acccaacttt atcattcctt aattcgtcat tcactaggac tgattggtaa
    14281 ttcgtcttct ggtttaatag aagttccttc tttgatgaaa cctactctta atattggtga
    14341 tcgccaaaaa ggtaggctac atggagaaag tgtagtaagt gtaccagtcg aaacctcctc
    14401 tgtattggag ggattatcaa aattgaatga agtgaccaac tttgacaatc cctactacaa
    14461 agagaatgct tcatctattg catatgaagc aataaagctg tatttgaaag atgaaccttc
    14521 aatttcccaa ccattttatg atttaaagga gaataatcta aaatgaaaaa aaagttggca
    14581 tttttaggcg caggaagtca tgctgatgcg attgctccgg ttattgatcc tcttgtgtat
    14641 gatttcgtag gtttttttga tgataaagat attacggagc atgatggtta tcctgttctt
    14701 ggaaaacttt atgatgtgct accttacctt gaagatggct caatagatgc agtatttatt
    14761 acaataggtg ataatgctaa aaggaaagaa ctatttgaat atgtagcaaa ggattattat
    14821 gactttatta ttaacatcat tagtcccaat gctttagtat tgacaccaga tagtatttgt
    14881 ggacgtggta tctttattgg ttttggggct tttataggtt ctaaagtgaa gctgtttgat
    14941 aacaatgttg ttaatacagg agcgctcatt gaacatcata ctgttgtaga atcacactgt
    15001 aatatagcac ctaacgctac cataaatggt ctttgttata ttagagaaga agtttatgta
    15061 ggtagtgcca gtgttattat tcaaaccttg gatatttcat cgtgtacgac aattggagca
    15121 ggagctgttg ttgtaaaaga tattatagaa cctggaacct atgtaggggt tccagccaaa
    15181 aaaataaaat aaaggatatg ttatgaagcc aatttgtatt attcctgcgc gatcagggtc
    15241 aaaaggatta ccagataaga atatgttatt cctagcaggt aaacccatga ttttccatac
    15301 tatagatgct gcaattgaat ctggaatgtt tgataagaaa gatatttttg ttagtacaga
    15361 ttcagaattg tacagagaga tttgtttaga acgcggtatt tcagtggtga tgagaaaacc
    15421 ggaactttca actgatcagg caacttcgta tgatatgtta aaagattttt tatctgacta
    15481 tgaagataat caggagtttg tgttacttca agtaacttct ccactaagaa aatcatggca
    15541 tataaaggaa gcaatggagt attattcttc acatgatgtt gacaatgttg taagtttttc
    15601 tgaagttgag aaacacccta gtctgtttac gacattgtct gataaaggct atgctataga
    15661 tatggtggga gcagataaag gttatcgtcg ccaagattta caacctttat actatccgaa
    15721 cggcgctatt tttatttcta ataaagaaac ttacttaagg gaaaaaagct ttttcacctc
    15781 taggacatat gcttatcaaa tggcaaagga attttcatta gatgttgata cgagagatga
    15841 ttttatccac gtcatcggtc acctcttttt cgattatgcc attagggaaa aagagaataa
    15901 agttttttat aaagaaggct atagtcgttt gttcaataga gaagcttcaa agataatttt
    15961 aggtgattca aaaacgatat ctacctcact agaaagttac cataattatt cccaaggtgg
    16021 tgtaacatta gcaacgatgt tggaaaactt acctaacttt ttgacagcta atgtaacaga
    16081 ggcttttgtt tctatagggg taaatgatct cattacaggt tatagtgtgg aagaaatatt
    16141 cagcaatttc caaaaactct actcattatt agctgagaat aaaataaaga tgagattgac
    16201 aactattgct tacacccttt ttagagaaac tgtcaataat gcagatattg agaaaatcaa
    16261 tcaatggcta acagaatttt gttatcaaaa tcagattcca ctgttagata ttaatagatt
    16321 tttatcaaag gatggtaatc taaattatca tttaactagt gatggattgc attttactca
    16381 agaggctaat gatttgttac aaagtcaata tcaattattt gtagacgaag ttaaaacctt
    16441 ataatatt
//
