LOCUS       AY376403               14202 bp    DNA     linear   BCT 29-APR-2005
DEFINITION  Streptococcus agalactiae strain 7271 Cps7C, Cps7D, Cps7E, Cps7F,
            Cps7G, Cps7H, Cps7M, Cps7I, Cps7J, Cps7K, Cps7L, NeuB, NeuC, NeuD,
            and NeuA genes, complete cds.
ACCESSION   AY376403
VERSION     AY376403.1
KEYWORDS    .
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14202)
  AUTHORS   Cieslewicz,M.J., Chaffin,D., Glusman,G., Kasper,D., Madan,A.,
            Rodrigues,S., Fahey,J., Wessels,M.R. and Rubens,C.E.
  TITLE     Structural and genetic diversity of group B streptococcus capsular
            polysaccharides
  JOURNAL   Infect. Immun. 73 (5), 3096-3103 (2005)
   PUBMED   15845517
REFERENCE   2  (bases 1 to 14202)
  AUTHORS   Cieslewicz,M.J., Madan,A., Wessels,M.R., Kasper,D. and Glusman,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2003) Channing Laboratory, Brigham and Women's
            Hospital, 181 Longwood Ave, Boston, MA 02115, USA
FEATURES             Location/Qualifiers
     source          1..14202
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /strain="7271"
                     /serotype="VII"
                     /db_xref="taxon:1311"
     CDS             128..820
                     /note="capsular polysaccharide chain length regulator"
                     /codon_start=1
                     /transl_table=11
                     /gene="Cps7C"
                     /product="Cps7C"
                     /protein_id="AAR25945.1"
                     /translation="MNKIANTEVEINIFNLLKKLWKKKFLITFVAIAFATAGLFYSLF
                     IVTPQYTSSTRIYVINPNTPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKL
                     NYPSSQLLQKITVSILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDI
                     TTLEKGNLPKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGL
                     TSLGIVPDLNKL"
     CDS             831..1529
                     /note="capsular polysaccharide chain length regulator"
                     /codon_start=1
                     /transl_table=11
                     /product="Cps7D"
                     /gene="Cps7D"
                     /protein_id="AAR25946.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAITSVR
                     EGEGKSTASTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVNESVATYGDY
                     GDYGNYGKRDRKRK"
     CDS             1581..2930
                     /note="putative glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /gene="Cps7E"
                     /protein_id="AAR25947.1"
                     /translation="MIQTVVVYFSASLTLTLITPNFKSNKDLLFVLLIHYIVFYLSDF
                     YRDFWSRGYLEEFKMVLKYSFYYIFISSSLFFIFKNSFTMTRLSFFPFIAMNSILLYL
                     LNSFLKYYRKYSYAKFSRDTKVVLITNKDSLSKMTFKNKYDHNYIAVCILDSSEKDCY
                     DLKHNSLRIINKDALTSELTCLTVDQAFINIPIELFGKYQIQDIINDIEAMGVIVNVN
                     VEALSFDNIGEKRIQTFEGYSVITYSMKFYKYSHLIAKRFLDIMGAIIGLLICGIVAI
                     FLVPQIRKDGGPAIFSQNRVGRNGRIFRFYKFRSMRVDAEQIKKDLLVHNQMTGLMFK
                     LDDDPRITKIGKFIRKTSIDELPQFYNVLKGDMSLVGTRPPTVDEYEKYNSTQKRRLS
                     FKPGITGLWQISGRNNITDFDEIVKLDVQYINEWSIWSDIKIILLTLKVVLLGTGAK"
     CDS             2954..3403
                     /note="beta-1,4-galactosyltransferase enhancer"
                     /codon_start=1
                     /transl_table=11
                     /gene="Cps7F"
                     /product="glycosyltransferase"
                     /protein_id="AAR25948.1"
                     /translation="MKICLVGSSGGHLAHLNFLKSIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     IYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     CDS             3403..3894
                     /note="beta-1,4-galactosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="Cps7G"
                     /product="glycosyltransferase"
                     /protein_id="AAR25949.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNCYMREAEIVITHGGPATFMNAVSKGKKTIVVPRQEQFGEHVNN
                     HQVDFVNKVKLIYNFDIIIDIEGLQNLVYEGAMNRPFLETNNSNFIKEFKKILKELCD
                     ENQ"
     CDS             3881..4975
                     /note="capsular polysaccharide repeat unit polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="Cps7H"
                     /product="polymerase"
                     /protein_id="AAR25950.1"
                     /translation="MKINKNSLFYIAIFLVNFFKSLGLGEENSTYKIVMLVAILLCAI
                     KFLLDSLYFERRKLVTIFLLFVATILNLFFVHKVTFILTLIFSLALKDISLKKAFSII
                     IGSRILGVLLNQIFVKLDFVEIKYVNFYRDGHFILRSDLGFGHPNFIHNFFALTFFLY
                     IVLNYKRLKLIVIVLFLTLNYLLYQYTFSRTGYYIVILFIVLIYLTKNSLIKRVFMKL
                     APYIQFFLLAFTFLSSTIFFNSNFVQKLDKLLTGRLNYAHLQLVDGLTLFGNSFKETS
                     VLFDNSYSMLLSMYGVVLTLFCMIIYYVYSKEIRIVELQLLLFIMSIVLFTESFYPSI
                     VMNISWLVFGKIFCDGIEPIKKEFTIIDDI"
     CDS             5013..5738
                     /note="beta-1,4-glucosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="Cps7M"
                     /product="glycosyltransferase"
                     /protein_id="AAR25951.1"
                     /translation="MIPKVIHYCWFGGNPLPDNLKKYIKTWREQCPDYEIIEWNERNY
                     DVTKNIFMREAYTKKNFAYVSDYARLDIIYTYGGFYLDTDVELLKSLDPLRVHNCFLA
                     RELSYDVNTGLIIGAIRGQKFIKDNMSVYEESKFLSFDKTCVEITTSLLLKKGLKSKN
                     IIQVIDGVTIYPRQYFNPKNLLTGKLDFLTNDTYSIHHYEGSWKKPSIFPDSMKIRVR
                     LVIDFIFGYGTYRRLLRFLKLKQ"
     CDS             5782..6744
                     /note="beta-1,6-N-glucosaminyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="Cps7I"
                     /product="glycosyltransferase"
                     /protein_id="AAR25952.1"
                     /translation="MKVSLVIPVYNGAPYIEETLESVMATTYSNLGNINCCDGSTDYT
                     SVICQKYTSKDKRVKYLYKENGGQSSARNLGVKLAEGDWIAFLDSDDLCSPFYIEYLV
                     RVQSETGADLVITGIEDRPNHRMEKLTCDKSTYSTLSVEATLTEMYYDNYGISPLGKL
                     YSKALLLRHPYPIGKIHEDTDTTYKLVASSTRIAFSKEKTVYIKGQPNSTTRQSYREN
                     MLYYFDAMQHNRDFILERYSDKELIDALNYKTVIGGMWIVNKLLEVDKYDYINSLRSE
                     YRKYYKSILFNKRVTIKEKIKYLSFISSMNLFLFLRKIYNYSEG"
     CDS             6748..7713
                     /note="beta-1,4-galactosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="Cps7J"
                     /product="glycosyltransferase"
                     /protein_id="AAR25953.1"
                     /translation="MKKISVIIPVYNVQSFLNECIDSVLAQTYSNLEIILVDDGSTDT
                     SGDICDYYSKKDRRILVFHKNNGGLSDARKKENSRATGDYIYLLDSDDYLYKEDAIEE
                     MVGCSKGYDSDIVLACYVERIGQRIINIVLENETIETISPLQAIRNIYNYDDYKAIFT
                     VAHNKLYKRELFSTMCYPVGKLHEDEFLTYKLYLKAKNIIFFRYNTYVYRIREDSIMT
                     GSYNVKRLHAIEALQERIHLLKEYPELVFQSEKALIKTMEVNLTELYKNDFYKEFYTL
                     RNEYQRTILDFIEKQGVLLKVKYSLKYCILYFKILNYKRKKNMNK"
     CDS             7710..8666
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="Cps7K"
                     /product="glycosyltransferase"
                     /protein_id="AAR25954.1"
                     /translation="MNYFVCHTLYHLLITIIKLKDKENTRIFICDTIPDYGAWIKILN
                     NQGIKTELFKELDYREQLQNKNVEEVMNLVDSYLKHYFERVNTQVYLFNDDTLMGRYM
                     AYLGKNYHLIEDGYNCFQAKLFFGGSVVKRIIKTYFLKKYVPYGFSKYCLSIEVNSLE
                     GLPHDRRSKKYKELPRKELFDSLTQEQKSLIFKLFKMKPITIAPKSVLLLTQPLAQDK
                     WYKTATERFQSIQEQYDYFEGIVHDYRERGYNIYLKVHPRDAVDYSKLPVELLPSNIP
                     MEIIELMLTGRFEVGITHSSTALDFLTCVDRKIILVNLKDIK"
     CDS             8663..10063
                     /note="capsular polysaccharide repeat unit transporter"
                     /codon_start=1
                     /transl_table=11
                     /gene="Cps7L"
                     /product="flippase"
                     /protein_id="AAR25955.1"
                     /translation="MNTKKLLQSGFIYTIGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMGIIALFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIVLLVAFLFR
                     SFLAPLVGFSESIFLLMICQSYANYVVTFFGQYFIQQQRSFANLILSLTNAVSSVALS
                     LFLIFHWSDDFLSRVFGAFVPTIIVAIVAFGYIYYHSKSFYNSKYFRFIITVSVPLIF
                     HLLGHQLLNQLDRIMLARLYNTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     AKLLQLPTYISRYLYLGVFLTLGYLTIFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPNYAAYGAAMATVISYLALLI
                     FHHIVSKVKYHYLDVSVRQYIILSGIVFSYAMLMNMFLDNIVVRWGLGIMILIVYSIV
                     FQKVILDLLSKKRRRR"
     CDS             10063..11088
                     /note="N-acetylneuraminic acid synthetase"
                     /codon_start=1
                     /transl_table=11
                     /gene="neuB"
                     /product="NeuB"
                     /protein_id="AAR25956.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEIRDYALSKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAIGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLPEL"
     CDS             11165..12319
                     /note="UDP-N-acetylglucosamine-2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="neuC"
                     /product="NeuC"
                     /protein_id="AAR25957.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIVLEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETSSVLEG
                     LSKLNEVTNFDNPYYKGNASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     CDS             12316..12945
                     /note="putative acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="neuD"
                     /product="NeuD"
                     /protein_id="AAR25958.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     CDS             12956..14197
                     /note="CMP-N-acetylneuraminic acid synthetase"
                     /codon_start=1
                     /transl_table=11
                     /gene="neuA"
                     /product="NeuA"
                     /protein_id="AAR25959.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPGLFTTLSDKGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSRTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLENYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGYSVEEIFSNFQKLYSLLAENKIKMRLTT
                     IAYTLFRETVNNADIEKINQWLTEFCYQNQIPLLDINRFLSKDGNLNYHLTSDGLHFT
                     QEANDLLQSQYQLFVDEVKTL"
BASE COUNT     4903 a   1939 c   2542 g   4818 t
ORIGIN
        1 ttagaccgcc atttttagca gaagcttata agattatctg tagagatttc ggtaaagaac
       61 gtgctaacca actttttatt gaaaatgctc aatctatatt aaaaaaccat tacatttagg
      121 agatttcatg aataaaatag ctaatacaga agttgaaata aatatattca accttttaaa
      181 aaaattgtgg aaaaagaaat ttttaatcac atttgttgcc attgcatttg caacagctgg
      241 acttttctat agtcttttta ttgttacgcc acaatatact tcttcaacaa ggatatatgt
      301 cattaaccct aataccccta ataatagtat tactgcacaa gatctacaag cggggagttt
      361 tcttgcaaat gactataagg agattattac gtctactgac gttctagaaa aagttatttc
      421 ttctgaaaaa ttgaattatc cttcgtctca gttgttgcaa aaaataacag tttctatttt
      481 aaaagataca cgtgttattt caatatcggt cgaagatgct aatccaaaaa tgtctcaaaa
      541 attagcaaat tcagttagag aagcagcagt ttcaaaaatc aaggcagtta ctcaagtaga
      601 ggatatcact actcttgaga agggaaattt acctaaagca ccatcttctc ctaatattaa
      661 aaagaatgta ctaatcgggt ttattgttgg tgcaggatta tcaactattg ttttagttat
      721 tatgggtatt ttggatgatc gtgttaatac tgaagaagat attgaaaagg tcctaggact
      781 tacttcttta ggtatagtac cagatttgaa taaactttaa ggagaatata atgactcgtt
      841 tagaaatagt tgatagcaag ttaagacaag caaaaaaaac agaagaatac ttcaatgcga
      901 tccgtacaaa catacagttt agtggaaagg aaaataaaat tcttgcaatt acctctgtta
      961 gggaagggga aggaaaatcc actgcttcaa caagtttagc tttatcttta gctcaagcag
     1021 gatttaaaac attattaatt gatgcggata ctaggaactc tgttatgtct ggaaccttta
     1081 aagcaactgg aactattaaa ggcttgacga attatttatc aggtaatgca gatcttggag
     1141 atattatctg tgaaaccaat gttcctagac tgatggtcgt tccttcaggg aaagtaccac
     1201 caaatccaac agcattactt cagaacgctt attttaataa gatgattgaa gctattaaaa
     1261 atatatttga ttatattatc atcgatactc cacctattgg tttagttgtt gatgccgcaa
     1321 taatcgctaa tgcttgcgat ggttttattt tagtaaccca agcaggcaga ataaaacgta
     1381 attatgttga aaaagcaaaa gaacagatgg aacaaagtgg ttcaaagttc ttaggtatta
     1441 ttcttaataa agttaatgaa tctgttgcta cttacggcga ttacggcgat tatggaaatt
     1501 acggaaaaag ggatagaaaa aggaagtaag gggctcttgt attgaaagaa aaagaaaata
     1561 tacaaaagat tattatagcg atgattcaaa cagttgtggt ttatttttct gcaagtttga
     1621 cattaacatt aattactccc aattttaaaa gcaataaaga tttattgttt gttctattga
     1681 tacattatat tgtcttttat ctttctgatt tttacagaga cttttggagt cgtggctatc
     1741 ttgaagagtt taaaatggta ttgaaataca gcttttacta tattttcata tcaagttcgt
     1801 tattttttat ttttaaaaac tcatttacaa tgacacgact ttcctttttt ccttttattg
     1861 ctatgaattc gattttatta tatctattga attcattttt aaaatattat cgaaaatatt
     1921 cttacgctaa gttttcacga gataccaaag ttgttttgat aacgaataag gattctttat
     1981 caaaaatgac ctttaagaat aaatacgacc ataattatat cgctgtctgt atcttggact
     2041 cctctgaaaa ggattgttat gatttgaaac ataactcgtt aaggataata aacaaagatg
     2101 ctcttacttc agagttaacc tgcttaactg ttgatcaagc ttttattaac atacccattg
     2161 aattatttgg taaataccaa atacaagata ttattaatga cattgaagca atgggagtga
     2221 ttgtcaatgt taatgtagag gcacttagct ttgataatat aggagaaaag cgaatccaaa
     2281 cttttgaagg atatagtgtt attacatatt ctatgaaatt ctataaatat agtcacctta
     2341 tagcaaaacg atttttggat atcatgggtg ctattatagg tttgctcata tgtggcattg
     2401 tggcaatttt tctagttccg caaatcagaa aagatggtgg accggctatc ttttctcaaa
     2461 atagagtagg tcgtaatggt aggattttta gattctataa attcagatca atgcgagtag
     2521 atgcagaaca aattaagaaa gatttattag ttcacaatca aatgacaggg ctaatgttta
     2581 agttagacga tgatcctaga attactaaaa taggaaaatt tattcgaaaa acaagcatag
     2641 atgagttgcc tcaattctat aatgttttaa aaggtgatat gagtttagta ggaacacgcc
     2701 ctcccacagt tgatgaatat gaaaagtata attcaacgca gaagcgacgc cttagtttta
     2761 agccaggaat cactggtttg tggcaaatat ctggtagaaa taatattact gattttgatg
     2821 aaatcgtaaa gttagatgtt caatatatca atgaatggtc tatttggtca gatattaaga
     2881 ttattctcct aacattaaag gtagtcttac ttgggacagg agctaagtaa aggtaaggtt
     2941 tgaaaggaat ataatgaaaa tttgtctggt tggttcaagt ggtggtcatc tagcacactt
     3001 gaactttttg aaatccattt gggaaaaaga agataggttt tgggtaacct ttgataaaga
     3061 agatgctagg agtattctaa gagaagagat tgtatatcat tgcttctttc caacaaaccg
     3121 taatgtcaaa aacttggtaa aaaatactat tctagctttt aaggtcctta gaaaagaaag
     3181 accagatgtt atcatatcat ctggtgccgc tgtagcagta ccattctttt atattggtaa
     3241 gttatttggt tgtaagacca tttatataga ggttttcgac aggatagata aaccaacttt
     3301 gacaggaaaa ttagtgtatc ctgtaacaga taaatttatt gttcagtggg aagaaatgaa
     3361 aaaagtttat cctaaggcaa ttaatttagg aggaattttt taatgatttt tgtcacagtg
     3421 gggacacatg aacagcagtt caaccgtctt attaaagaag ttgatagatt aaaagggaca
     3481 ggtgctattg atcaagaagt gttcattcaa acgggttact cagactttga acctcagaat
     3541 tgtcagtggt caaaatttct ctcatatgat gatatgaact gttacatgag agaagctgaa
     3601 atcgttatta cacatggcgg tccagcgacg tttatgaatg cagtttctaa ggggaaaaaa
     3661 accatagtag ttcccagaca agaacagttt ggagagcatg tgaataatca tcaggtagat
     3721 tttgttaata aggtcaagtt gatctataat tttgatatta ttatagacat tgaagggtta
     3781 caaaatctag tttatgaagg agcgatgaat cgtccgtttt tagaaaccaa caatagtaat
     3841 tttattaaag aatttaagaa gatattaaag gagttgtgtg atgaaaatca ataaaaactc
     3901 tttattttat attgcaatat ttttagttaa tttttttaaa tcactaggtt taggagagga
     3961 aaattcaact tacaaaatag taatgttagt tgcaatctta ttgtgtgcaa taaaattttt
     4021 actcgatagc ctttattttg aaagaagaaa actcgttact atcttcttgt tatttgtcgc
     4081 gactattttg aatttatttt ttgtacataa ggttactttt atattaactt taattttttc
     4141 cctagcattg aaggatatct ctctaaaaaa agctttctct ataataatag gatcgcgtat
     4201 attgggagtt ctattaaatc aaatttttgt gaaattagat tttgtagaaa taaagtatgt
     4261 caatttttat agagatggac atttcattct gagaagtgac ttaggttttg ggcatcccaa
     4321 ctttattcat aatttttttg ctctaacctt ttttttgtat attgtactca attataaacg
     4381 actgaagctt attgtaatag ttttattttt aacattaaat tatttactgt atcaatatac
     4441 tttttcaaga acagggtatt atattgtaat tttatttatt gtactcattt atctaacaaa
     4501 gaatagtcta ataaaaagag tatttatgaa attagcacca tatatacagt tttttttatt
     4561 agcatttact tttttgagtt ctacaatttt tttcaattca aattttgttc aaaaattaga
     4621 taagcttttg acaggtaggt taaactatgc tcatttacaa cttgtagacg gtttgactct
     4681 ttttggaaat agttttaagg aaacaagtgt cctgtttgat aatagctact ctatgttatt
     4741 gagtatgtat ggtgtagtcc ttaccctgtt ttgtatgata atctattatg tttacagtaa
     4801 agaaataaga atagttgaac ttcaattact gctatttata atgtctatag tattatttac
     4861 agagagtttt tacccaagta tagttatgaa tattagttgg ttagtttttg ggaaaatatt
     4921 ttgtgatggt attgaaccaa taaaaaaaga atttactatt atagacgata tatgatatat
     4981 ttattctgat atagtaggat gtgaggaaac aaatgatacc taaagttata cattattgtt
     5041 ggtttggagg aaatccctta cctgataatt taaaaaagta cataaaaacc tggagagaac
     5101 aatgtccaga ttacgaaatt attgaatgga acgaacgtaa ttatgatgtt accaaaaata
     5161 tttttatgag agaagcatat actaagaaaa attttgccta tgtttctgat tatgcaaggt
     5221 tggatattat ttacacttat ggcggattct atctagacac tgatgtggaa cttttaaaaa
     5281 gtttagaccc tttgagagtt cataactgtt ttttagctag ggagttaagt tatgatgtga
     5341 atacaggact aattattggt gcaattagag gacaaaaatt tataaaagac aatatgtcgg
     5401 tatacgaaga atctaaattt ttaagttttg ataaaacatg tgtagaaatc acgaccagct
     5461 tacttttaaa aaaaggactg aaaagtaaaa atatcataca agttattgat ggtgtcacta
     5521 tttatccaag acaatacttt aatcctaaaa atttgttgac aggaaagcta gattttctaa
     5581 caaatgatac ttattctata catcattatg aaggaagttg gaaaaagcca tctatttttc
     5641 ctgattcgat gaaaattaga gtaagacttg taattgattt tatatttgga tatggaacat
     5701 atagaaggct gttgcgtttt ttaaagttaa aacagtagtc gctattaact gaaatatttt
     5761 ctttaaagaa atggaggaac aatgaaagta tcattagtca taccagttta taatggggca
     5821 ccttacatcg aagaaacatt agaaagtgtc atggccacaa cttattcaaa tctaggaaat
     5881 attaattgtt gtgatggctc aactgattac acatcagtta tatgccaaaa atacacttct
     5941 aaagataaac gcgtgaaata tttatataaa gaaaatggcg gtcaatcctc tgctagaaat
     6001 ttaggagtca aactagctga aggagattgg attgctttct tggattccga tgacctctgc
     6061 tcaccattct atatagaata tttagtaaga gtgcagagtg aaactggtgc tgatttggtg
     6121 ataactggaa tcgaagaccg ccccaaccat agaatggaaa aacttacatg tgataagtca
     6181 acttactcaa cactcagtgt ggaagcgaca ttaacagaaa tgtactatga taactacggt
     6241 atttctccat tgggaaagtt atactctaaa gcattattat taagacatcc ataccctatt
     6301 ggtaaaattc atgaagatac agatacaact tacaaactgg ttgcaagtag tacacgaatt
     6361 gcgtttagca aagagaaaac agtatatata aaaggtcaac ctaatagtac gactaggcag
     6421 tcatataggg agaacatgtt atattatttt gatgcaatgc aacataatag agactttatt
     6481 cttgaacgtt attcagacaa agaactaatc gatgctttaa attataaaac tgtaattgga
     6541 ggaatgtgga tcgttaataa gcttctagaa gttgataaat acgattatat taattctttg
     6601 agaagtgaat atagaaaata ttacaagagt atattattta ataaacgtgt gacaataaaa
     6661 gaaaaaataa aatatctgtc atttatttcc tcaatgaacc tgtttttatt cttaagaaag
     6721 atttacaact atagtgaggg ataaattatg aaaaagatca gtgttattat ccccgtttac
     6781 aatgttcaat cttttctaaa tgaatgtata gatagtgttt tggctcaaac gtattcaaat
     6841 ttagaaatta ttttagttga tgatggctct acggatactt ctggggatat ctgtgactat
     6901 tattcaaaaa aagatagacg tatattagtt tttcataaga ataatggtgg cttatctgat
     6961 gccagaaaaa aagaaaatag tagagctact ggagattata tttatttact tgactctgat
     7021 gattatttat ataaagaaga tgcaattgag gaaatggttg gatgtagtaa gggatatgat
     7081 tcagatattg ttttagcatg ttatgttgaa agaataggac aacgaattat aaatattgtt
     7141 ttagagaatg aaacaataga aacaattagt ccgctacaag caatacgaaa tatttataat
     7201 tatgatgact ataaagctat ttttactgta gcacataata aattatataa gagagaatta
     7261 ttttctacaa tgtgttaccc agtaggaaaa ctacacgaag atgaatttct aacttataaa
     7321 ttatatttga aagcaaaaaa tataattttc tttcgatata acacttatgt atatcgtatt
     7381 agagaagaca gtataatgac tggttcttac aatgttaaaa gattacatgc tatagaagca
     7441 ctccaggaaa gaattcattt attaaaagag tacccagagt tagtatttca aagtgaaaaa
     7501 gctttaatta aaactatgga agtaaacttg acagagttat acaaaaatga tttttataag
     7561 gaattttata ctctaaggaa tgaatatcag agaactattc ttgattttat agaaaaacaa
     7621 ggtgtgctac taaaagtaaa atattcttta aaatattgca tattgtattt taaaatatta
     7681 aattataaaa gaaagaaaaa tatgaataaa tgaattattt tgtgtgtcat actttatatc
     7741 atctactgat tactataata aagctaaaag ataaagaaaa cactagaata tttatttgtg
     7801 atactatccc tgattatgga gcttggataa aaatattaaa taatcaggga ataaaaactg
     7861 aattatttaa agagctagat tatcgggaac agttacaaaa taaaaacgtt gaagaagtga
     7921 tgaaccttgt agattcatat ttaaaacact actttgagag agtaaataca caagtctacc
     7981 tatttaatga tgatacctta atggggcgtt atatggcata tttaggtaaa aactatcatc
     8041 taatcgaaga tggatataac tgttttcaag ctaagttatt ctttgggggt tccgttgtaa
     8101 aacgaataat taaaacctac tttttgaaaa aatatgttcc atatggtttt tcaaaatatt
     8161 gtctttcaat tgaggtaaat tcattagagg gcttacctca cgacaggaga agtaaaaaat
     8221 ataaagaact accgagaaaa gaattatttg atagtcttac ccaagaacaa aagtctctga
     8281 ttttcaaact ttttaagatg aaaccgataa ctatagctcc aaagtcagta ttgttattaa
     8341 cgcagccact tgcacaggat aaatggtata aaacagctac agaaaggttt caaagtattc
     8401 aggagcaata cgattatttt gaggggattg tccacgacta tagagaacga gggtacaata
     8461 tttatttaaa agttcaccct agagatgcag tagattattc caaattgcca gtggagttac
     8521 taccatcaaa tatccctatg gaaattatag agttgatgtt gacaggtcgt ttcgaggttg
     8581 ggataacgca ctcatccact gcactggatt ttttaacttg tgttgataga aaaataattt
     8641 tagtgaatct gaaggatatt aaatgaatac taagaaatta cttcaatctg gttttattta
     8701 tacaattgga aatttattgg tgcaagggct agcctttatc acacttccta tttacactcg
     8761 ggtcatctca acagaagttt atggtcaata tagtttatat gtggcttgga tggggatcat
     8821 tgcacttttt attggtcttc agacgagcgg ttctttaagt tcagcccggg tcaaatatgg
     8881 agaagagttt aaaagctatt ctggaagtgc gttttccgta ggtaatatat ggtttcttat
     8941 tgtacttttg gtagcttttc tatttagaag ttttttggca ccattagttg gtttttctga
     9001 atctattttt ttattaatga tatgtcagag ttatgctaac tatgttgtaa ctttctttgg
     9061 ccaatatttt atacagcaac agaggagttt tgcaaattta atactatcct taactaatgc
     9121 tgtttcatct gttgcattat cactattttt gatttttcat tggtctgatg attttctgtc
     9181 tagggttttt ggagcttttg ttcctactat aatagttgca atagttgcct tcggttatat
     9241 ttattatcat agtaaatctt tttacaattc taagtatttt cggttcatta taacagtatc
     9301 tgttcctttg atttttcatt tgttaggaca tcaattatta aaccaactag atagaatcat
     9361 gctagctcgt ttatataata ctaaagaagt cgcaatgtat agttttggct attcacttgg
     9421 gatgattatt cagattgttt tgaatagcat taatatggct tggattccat ggttttttga
     9481 tgctagaaaa gcaaaattac tacagcttcc aacttatatt agccgttatc tgtacttagg
     9541 agttttctta actttaggct atttgactat tttccctgaa ttagctcaaa taatgggagg
     9601 agacaagtat agtagtagtg ttcaatttat ctctttaatt attgtcagct attttttggt
     9661 ctttctatat acattccctg taaatatcca atttttctat gctaatacga catggattcc
     9721 tattggaaca ttattagctg ctggtgtaaa ttggttacta aatcttgtat taattcctaa
     9781 ctatgcagcc tatggcgctg ctatggctac agtaatatct tatttagctt tgttgatttt
     9841 tcatcatatt gtttcgaagg ttaaatacca ttatttagat gtctcagtca ggcagtacat
     9901 cattttaagt ggtatagtat ttagttatgc tatgttaatg aacatgttcc tagataatat
     9961 cgtggtgaga tggggtttag ggataatgat tcttatagta tacagcattg tttttcaaaa
    10021 agtcatctta gacttattga gtaaaaaaag aaggagaaga taatggttta tattattgca
    10081 gagattggtt gcaatcacaa tggagatatt aatcttgcga aaaaaatggt agatgttgcc
    10141 gtatcttgcg gtgttgatgc tgttaaattt cagactttta aagctgagaa acttatttct
    10201 aaatttgcac ctaaagctga atatcaaaaa gcaactacag gaacagcaga cagtcaactt
    10261 gaaatgacga aacgtttgga gttaagtttt gaagaatatt tagaaatacg tgattatgca
    10321 ctttcaaaag gcgtagagac cttttcaaca ccttttgatg aggaatcatt agagttctta
    10381 atttctacgg atatgccaat ttacaaaatt ccatcaggag aaatcaccaa tttaccttac
    10441 ttagaaaaga ttggcaagca acaaaagaaa gttattcttt cgacgggtat ggcggtaatg
    10501 gaagagatcc atcaagcggt gaatatttta cgtcagaatg gtacaaccga catttctatt
    10561 ttacattgta caacagagta cccaacacct tacccctctc taaatttaaa cgttattcat
    10621 actttgaaag atgaatttaa agatttaacg ataggttatt cggatcattc aattggatca
    10681 gaagtaccta tcgcagcagc agcaataggt gcagaagtta ttgaaaaaca ctttacttta
    10741 gatactaata tggaaggtcc ggatcataaa gccagtgcaa cacctgatat tttagctgct
    10801 ttagttaaag gggttcgcat tgttgaacaa gccttaggta gatttgaaaa aatcccagat
    10861 ccagtagaag agaaaaataa gattgttgct cgtaaatcag tcgttgcttt aaaaccaatt
    10921 aaaaaaggcg atatttattc aatagaaaat attacggtga agcgcccagg taatggtatt
    10981 tctcctatga actggtatga catcttggga caagaagcgc aagatgattt cgaagaggat
    11041 gaagttattc gtgattcacg ctttgaaaat caattgcccg agttataata acacctaatt
    11101 ataatcggca acagatgtat tacacggcta agattatgta attactatat atagaaggga
    11161 tacgatgaaa aaaatttgtt tagtaaccgg atcgcgtgct gaatacggaa taatgaagcc
    11221 attgattcaa agattatcaa aagataaaga agtcaactta caaattattg caacagcaat
    11281 gcatctggaa gaaaaatacg gctatactta tcgtcaaatt gaagaagacg gttttgatat
    11341 tgcttataaa gttcccttac atctttatga tactgacaga agaactgtat ctactgcaat
    11401 ggcgcattta caactaggat tgaccaaaat ttttgacaag gaagactatg atctagtcat
    11461 cattttaggg gatcgttacg aaatgttacc agttgtgaat gtagcgttga tttataatgt
    11521 cccagtatgc caccttcatg gaggggagac ctcattaggc aattttgatg agtatattcg
    11581 ccatgcaatt actaagatga gtcacctaca cttagtctct acagaggact ttcgtcaacg
    11641 tgtgattcag atgggagaac aacctcaatt tgtaattaac acaggagctc tcggagtgga
    11701 aaatgctcta tcaatccctt ctctaaccaa agaagcaata gaaaagcaat taggtattgt
    11761 actagaggaa tcctattttg ttgttctcta tcatcctgtt acttttgaac aaggaaaaag
    11821 tgctggagaa cagatgaaag cggtactatc agccctttct aaatttggtg tccagtgtct
    11881 atttatagga tccaattcag atactggttc tgatgatatt gcaaaggcta tcaatactta
    11941 tttgataaat catgaaaata gttattgctt tgcttcttta tcaacccaac tttatcattc
    12001 cttaattcgt cattcactag gactgattgg taattcgtct tctggtttaa tagaagttcc
    12061 ttctttgatg aaacctactc ttaatattgg tgatcgccaa aaaggtaggc tacatggaga
    12121 aagtgtagta agtgtaccag tcgaaacctc ctctgtattg gagggattat caaaattgaa
    12181 tgaagtgacc aactttgaca atccctacta caaagggaat gcttcatcta ttgcatatga
    12241 agcaataaag ctttatttga aagatgaacc ttcaatttcc caaccatttt atgatttaaa
    12301 ggagaataat ctaaaatgaa aaaaaagttg gcatttttag gcgcaggaag tcatgctgat
    12361 gcgattgctc cggttattga tcctcttgtg tatgatttcg taggtttttt tgatgataaa
    12421 gatattacgg agcatgatgg ttatcctgtt cttggaaaac tttatgatgt gctaccttac
    12481 cttgaagatg gctcaataga tgcagtattt attacaatag gtgataatgc taaaaggaaa
    12541 gaactatttg aatatgtagc aaaggattat tatgacttta ttattaacat cattagtccc
    12601 aatgctttag tattgacacc agatagtatt tgtgggcgtg gtatctttat tggttttggg
    12661 gcttttatag gttctaaagt gaagctgttt gataacaacg ttgttaatac aggagcgctc
    12721 attgaacatc atactgttgt agaatcacac tgtaatatag cacctaatgc taccataaat
    12781 ggtctttgtt atattagaga agaagtttat gtaggtagtg ccagtgttat tattcaaacc
    12841 ttggatattt catcgtgtac gacaattggc gcaggagctg ttgttgtaaa agatattata
    12901 gaacctggaa cctatgtagg ggttccagcc aaaaaaataa aataaaggat atgttatgaa
    12961 gccaatttgt attattcctg cgcgatcagg gtcaaaagga ttaccagata agaatatgtt
    13021 attcctagca ggtaaaccca tgattttcca tactatagat gctgcaattg aatctggaat
    13081 gtttgataag aaagatattt ttgttagtac agattcagaa ttgtacagag agatttgttt
    13141 agaacgcggt atttcagtgg tgatgagaaa accggaactt tcaactgatc aggcaacttc
    13201 gtatgatatg ttaaaagatt ttttatctga ctatgaagat aatcaggagt ttgtgttact
    13261 tcaagtaacc tctccactaa gaaaatcatg gcatataaag gaagcaatgg agtattattc
    13321 ttcacatgat gttgacaatg ttgtaagttt ttctgaagtt gagaaacacc ctggtctgtt
    13381 tacgacattg tctgataaag gctatgctat agatatggtg ggagcagata aaggttatcg
    13441 tcgccaagat ttacaacctt tatactatcc gaacggtgct atttttattt ctaataaaga
    13501 aacttactta agggaaaaaa gctttttcac ctctaggaca tatgcttatc aaatggcaaa
    13561 ggaattttca ttagatgttg atacgagaga tgattttatc cacgtcatcg gtcacctctt
    13621 tttcgattat gccattaggg aaaaagagaa taaagttttt tataaagaag gctatagtcg
    13681 tttgttcaat agagaagctt caaagataat tttaggtgat tcaaaaacga tatctatctc
    13741 actagaaaat taccataatt attcccaagg tggtgtaaca ttagcaacga tgttggaaaa
    13801 cttacctaac tttttgacag ctaatgtaac agaggctttt gtttctatag gggtaaatga
    13861 tctcattaca ggttatagtg tggaagaaat attcagcaat ttccaaaaac tctactcatt
    13921 attagctgag aataaaataa agatgagatt gacaactatt gcttacaccc tttttagaga
    13981 aactgtcaat aatgcagata ttgagaaaat caatcaatgg ctaacagaat tttgttatca
    14041 aaatcagatt ccactgttag atattaatag atttttatca aaggatggta atctaaatta
    14101 tcatttaact agtgatggat tacattttac tcaagaggct aatgatttgt tacaaagtca
    14161 atatcaatta tttgtagacg aagttaaaac cttataatat tt
//
