LOCUS       AY375363               12637 bp    DNA     linear   BCT 29-APR-2005
DEFINITION  Streptococcus agalactiae serotype VIII Cps8C, Cps8D, Cps8E, Cps8R,
            Cps8H, Cps8J, Cps8K, Cps8L, NeuB, NeuC, NeuD, and NeuA genes,
            complete cds.
ACCESSION   AY375363
VERSION     AY375363.1
KEYWORDS    .
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12637)
  AUTHORS   Cieslewicz,M.J., Chaffin,D., Glusman,G., Kasper,D., Madan,A.,
            Rodrigues,S., Fahey,J., Wessels,M.R. and Rubens,C.E.
  TITLE     Structural and genetic diversity of group B streptococcus capsular
            polysaccharides
  JOURNAL   Infect. Immun. 73 (5), 3096-3103 (2005)
   PUBMED   15845517
REFERENCE   2  (bases 1 to 12637)
  AUTHORS   Cieslewicz,M.J., Madan,A., Wessels,M.R., Kasper,D. and Glusman,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2003) Channing Laboratory, Brigham and Women's
            Hospital, 181 Longwood Ave, Boston, MA 02115, USA
FEATURES             Location/Qualifiers
     source          1..12637
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /strain="SMU014"
                     /serotype="VIII"
                     /db_xref="taxon:1311"
     CDS             128..820
                     /note="capsular polysaccharide chain length regulator"
                     /codon_start=1
                     /transl_table=11
                     /gene="cps8C"
                     /product="Cps8C"
                     /protein_id="AAR29920.1"
                     /translation="MNKIANTEVEINIFNLLKKLWKKKFLITFVAIAFATAGLFYSLF
                     IVTPQYTSSTRIYVINPNTPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKL
                     NYPSSQLLQKITVSILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDI
                     TTLEKGNLPKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGL
                     TSLGIVPDLNKL"
     CDS             831..1526
                     /note="capsular polysaccharide chain length regulator"
                     /codon_start=1
                     /transl_table=11
                     /gene="cps8D"
                     /product="Cps8D"
                     /protein_id="AAR29921.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAITSVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVNESVATYGDY
                     GDYGNYGKRDRKR"
     CDS             1542..2918
                     /note="undecaprenylphosphate glucosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cps8E"
                     /product="glycosyltransferase"
                     /protein_id="AAR29922.1"
                     /translation="MYNPDSSKKIYYLLSDIIANLLTYVLLAIFYHYSFFESSYFLII
                     FITLIVVSSVFMDEYTYITRRGYLKELKSSIIYGLKCIIFFSFVLSLGKFRFLSDIAV
                     LSYFFLLLFFLLNSLLVYTGRIMLKSIMRGREDKIKKILLLTNFTDNKSFEKALLQHH
                     YQVVGYVSSKVVDYRVVPVLRNVSDIRNFIANNQVDEIFVDIDAYSDYIEAARYFKLL
                     GIPTTINITNYNNYYIYNSILKKIGNMSFVTTAINIVSFRQLFLKRIIDIFIAVIGIF
                     ITGIVALLIFPIIKIQSPGPLFFTQKRVGKNGKVFEIYKFRSMYIDAEQRKKELLSKN
                     NLNTNLMFKMENDPRIFPFGQKIRNWSIDELPQFLNVLKGDMSVVGTRPPTLEEYKQY
                     ELHHFKRLAAKPGITGLWQVSGRSAITDFEEVVSLDMKYIQNWSLSEDIKIIVRTFVV
                     IFKKEGSR"
     CDS             2971..4176
                     /note="beta-1,4-rhamnosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cps8R"
                     /product="glycosyltransferase"
                     /protein_id="AAR29923.1"
                     /translation="MRRTVYIIGSKGIPARYGGFETFVEKLTEKHKNKDIKYFVACTR
                     ENSIKSNISENIFEYNGATCFNVDVPDIGSAKAVLYDILALRKAIAISKKNNDENPIF
                     YILACRIGPFISKYDKKIRQIGGQLFVNPDGHEWLREKWSIPVRRYWRISERLMVKYA
                     NLLICDSKNIEKYIQKDYSKYAPKTTYIAYGTDLTKSKHNFKDKVVRSWFDDKKISEN
                     NYYLVVGRFVPENNYESMIREFMKSKSKRDLVLITNIEQNVFYEKLKKETKFDKDNRI
                     KFVGTVYNQQLLSYIRENAFAYLHGHEVGGTNPSLLEALASTSINLLLDVNFNREVGG
                     NGALYWKKDTLNEVIKRCELLSRDSIEKLAKQSKKQVRKYFSWDLIIDAYEKLFYDID
                     SYFLAKGKH"
     CDS             4219..5367
                     /note="capsular polysaccharide repeat unit polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cps8H"
                     /product="polymerasse"
                     /protein_id="AAR29924.1"
                     /translation="MGIVLGGSQVFNNFAIDSSLKYLSYFILFLCIIVKLLRNPLTII
                     FSRLVYFICISFLFIIGIYLQDLPLHRIIYLSFSMIIISGLATLTVSLFDNVKDLRRV
                     SYYIFYGVLLSTILGIFYQVPMFTVAVEGVGATSLGFNGGMLHKNFYAITILTSYILL
                     FISRKYGVRHNIDSYVLWFEFFLILISNSRTVYLILIIFYCINNWKLLLRINKKQRIL
                     VFIPAIFISLIIAIIFFEYLTNNSDSYTYRVQGIINFFNYYQSDYFHLLFGDAQLAFG
                     GTTQNYTYNIRSVIGWNGTVEMPLLSIMIKNGFIGLLGYTVVLLSFFKNIKKIKNINI
                     KTVGLLVIIPLLMSATVENYIVNVNFIFMPICFCVLNSLKRLEHNLNV"
     CDS             5383..6360
                     /note="beta 1,4 galactosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cps8J"
                     /product="glycosyltransferase"
                     /protein_id="AAR29925.1"
                     /translation="MQNLVSIILPVYNVENYIENCLNSIISQSYFHFEVIIVNDGSTD
                     NSMSYCEKIVCEDLRFRIINKDNGGLSDARNVGLEAAKGDYIIFVDSDDFISPNLVSH
                     LMACLENYEADLAICDPVHFYLGGNQETHEKNIFKKHSNIHLLTNEEAICELFYQKTF
                     LVSAWGKVYKKSIFDNIRFPKGKLFEDSAVMHLLFEKSNKIVYSNAKLYAYVHRKDSI
                     TTSQFSERDLDILDITDQILKRYKSFPKIYQAAISYKISACFRVILNAPNIEKYTPIV
                     AECKKYVYYHWKDILFNKKTRIKTKLALISIVLFKPFIKFIYTHVDRWK"
     CDS             6441..7340
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cps8K"
                     /product="glycosyltransferase"
                     /protein_id="AAR29926.1"
                     /translation="MNLIMCRTPLQTIIAERIIEMFPNESFEVICYFESRNKKQAYYS
                     TRLKKKSELYTEIVFKNRAQEILTIIYFRFIFLRKYYDKIYIANITNVLFHTILSAST
                     FNKIFTFDDGLANIIKSSFLYASRTSLKAKFFKFVFLIKYDAQRIKNESSLHYTLYKN
                     NDNIIKNTKFISILRNITKHKDVSNNSISICIGQPLYSDDLMNIHYFNNIIHKYDIEY
                     YFPHPRETFRIDNIIYIETQCIFEEYVVNLLKEFSEINLYTCFSSAALNVIDIDGINV
                     FIIKSAEFEEEQSIFNEFKLTFV"
     CDS             7337..8761
                     /note="capsular polysaccharide repeat unit transporter"
                     /codon_start=1
                     /transl_table=11
                     /gene="cps8L"
                     /product="flippase"
                     /protein_id="AAR29927.1"
                     /translation="MICSKGGRMNTKKLLQSGFIYTIGNLLVQGLAFITLPIYTRVIS
                     TEVYGQYSLYIAWMNIIMLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIV
                     LLIAFLFRSFLAPLVGFSESIFLLMVCQSYANYVVTFFGQYFIQQQRSLANLILSLAN
                     AVSSVALSLFLIFHWSDDFLSRVFGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIV
                     TVSVPLIFHLLGHQLLGQLDRIMLARLYNTKEVAMYSFGYSLGMIIQIVLNSINMAWI
                     PWFFDARKEKLLQLSTYISRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLI
                     IVSYFLVFLYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATI
                     ISYLALLIFHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLDNIVIRWSLGII
                     ILIVYSIVFQKVILDLLSKKRRRR"
     CDS             8761..9780
                     /note="N-acetylneuraminate synthetase"
                     /codon_start=1
                     /transl_table=11
                     /gene="neuB"
                     /product="NeuB"
                     /protein_id="AAR29928.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEMRDYAILKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLP"
     CDS             9863..11017
                     /note="N-acetylglucosamine-2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuC"
                     /gene="neuC"
                     /protein_id="AAR29929.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIALEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETSSVLEG
                     LSKLNEVTNFDNPYYKENASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     CDS             11014..11643
                     /note="putative acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD"
                     /gene="neuD"
                     /protein_id="AAR29930.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     CDS             11654..12625
                     /note="CMP-N-acetylneuraminic acid synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuA"
                     /gene="neuA"
                     /protein_id="AAR29931.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPSLFTTLSDEGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSKTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLENYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLIKGYSVWKIYSAISKNSTHY"
BASE COUNT     4314 a   1674 c   2194 g   4455 t
ORIGIN
        1 ttagaccgcc atttttagca gaagcttata agattatctg tagagatttc ggtaaagaac
       61 gtgctaacca actttttatt gaaaatgctc aatctatatt aaaaaaccat tacatttagg
      121 agatttcatg aataaaatag ctaatacaga agttgaaata aatatattca accttttaaa
      181 aaaattgtgg aaaaagaaat ttttaatcac atttgttgcc attgcatttg caacagctgg
      241 acttttctat agtcttttta ttgttacgcc acaatatact tcttcaacaa ggatatatgt
      301 cattaaccct aataccccta ataatagtat tactgcacaa gatctacaag cggggagttt
      361 tcttgcaaat gactataagg agattattac gtctactgac gttctagaaa aagttatttc
      421 ttctgaaaaa ttgaattatc cttcgtctca gttgttgcaa aaaataacag tttctatttt
      481 aaaagataca cgtgttattt caatatcggt cgaagatgct aatccaaaaa tgtctcaaaa
      541 attagcaaat tcagttagag aagcagcagt ttcaaaaatt aaggcagtta ctcaagtaga
      601 ggatatcact actcttgaga agggaaattt acctaaagca ccatcttctc ctaatattaa
      661 aaagaatgta ctaatcgggt ttattgttgg tgcaggatta tcaactattg ttttagttat
      721 tatgggtatt ttggatgatc gtgttaatac tgaagaagat attgaaaagg tcctaggact
      781 tacttcttta ggtatagtac cagatttgaa taaactttaa ggagaatata atgactcgtt
      841 tagaaatagt tgatagcaag ttaagacaag caaaaaaaac agaagaatac ttcaatgcga
      901 tccgtacaaa catacagttt agtggaaagg aaaataaaat tcttgcaatt acctctgtta
      961 gggaagggga aggaaaatcc actacttcaa caagtttagc tttatcttta gctcaagcag
     1021 gatttaaaac attattaatt gatgcggata ctaggaactc tgttatgtct ggaaccttta
     1081 aagcaactgg aactattaaa ggcttgacga attatttatc aggtaatgca gatcttggag
     1141 atattatctg tgaaaccaat gttcctagac tgatggtcgt tccttcaggg aaagtaccac
     1201 caaatccaac agcattactt cagaacgctt attttaataa gatgattgaa gctattaaaa
     1261 atatatttga ttatattatc atcgatactc cacctattgg tttagttgtt gatgccgcaa
     1321 taatcgctaa tgcttgcgat ggttttattt tagtaaccca agcaggtaga ataaaacgta
     1381 attatgttga aaaagcaaaa gaacagatgg aacaaagtgg ttcaaagttc ttaggtatta
     1441 ttcttaataa agttaatgaa tctgttgcta cttacggcga ttacggcgat tatggaaatt
     1501 acggaaaaag ggatagaaaa agataggttt tagaataaga aatgtacaat ccagattcct
     1561 caaaaaagat ttattatttg ttatcagata tcattgctaa tttactaacg tatgtattat
     1621 tagcaatttt ttatcattat tctttttttg aaagttcata tttcttaatt atttttataa
     1681 cactcattgt tgtatcatct gtttttatgg atgaatatac ttatattact agaagaggtt
     1741 atttaaaaga acttaaatca tctattatct atgggttaaa gtgtattatt ttcttttcat
     1801 tcgtacttag ccttgggaaa tttaggtttt taagtgatat cgccgtttta tcttattttt
     1861 tcttattatt attctttctt ctcaatagtt tattagttta tactggtcgt attatgctta
     1921 aatctattat gagagggaga gaggataaga taaaaaaaat tctattgtta actaatttta
     1981 cagataacaa atcttttgaa aaagcattac ttcaacatca ttatcaagtt gttggatatg
     2041 ttagttcaaa agttgttgat tatagagtag taccagtatt aaggaatgtc tctgatatac
     2101 gaaattttat tgctaataat caggtagatg agatttttgt agatattgat gcttattctg
     2161 attatattga ggcagcacga tattttaaac tacttggaat tccgacaact ataaatataa
     2221 caaattataa taactattat atttataata gtattttaaa aaaaataggt aatatgtcat
     2281 ttgtgacaac agcaattaat atcgttagtt ttagacaatt gtttttaaag agaataatag
     2341 atatttttat agcagttatt ggtattttta taacaggaat tgttgcatta ttaatttttc
     2401 ctatcataaa aatacaatca ccaggaccac tttttttcac gcagaaacgt gttggaaaga
     2461 acggaaaagt atttgaaatc tataaattta gaagtatgta tatagatgca gagcagcgaa
     2521 aaaaagaatt attatctaaa aataatctta atacgaattt gatgtttaaa atggaaaatg
     2581 atccaagaat ttttccattt ggacaaaaaa ttagaaattg gtctattgat gaacttcctc
     2641 aatttttaaa cgttttaaaa ggagatatgt ctgtggtagg tactcgtcct ccgacccttg
     2701 aggaatataa acaatatgag ttacatcatt ttaaacgatt agctgctaaa ccaggaatta
     2761 ctggtttatg gcaagttagt ggacgaagtg ccattactga ctttgaggaa gttgtttcat
     2821 tggatatgaa atatattcaa aattggtcat tgtcagaaga tattaagatt attgtaagaa
     2881 catttgtagt aatttttaaa aaagaaggaa gtagatagtg gaattattta ctctcttaaa
     2941 ttttataaaa tttaagagaa tggaggagtt atgagaagga cagtatatat tattggttca
     3001 aaaggtattc cggcaagata tggtggattt gaaacgtttg ttgaaaaact tacagaaaag
     3061 cataaaaata aagacatcaa atattttgta gcttgtacta gagagaattc tataaaatct
     3121 aatatatcag aaaatatttt tgaatataat ggtgctactt gctttaatgt tgatgttcca
     3181 gatattggtt cagctaaagc ggtgctgtat gatattctgg ctttaagaaa ggcgattgct
     3241 atttctaaaa aaaacaatga tgaaaatccc atattttaca ttttagcttg tcgtataggt
     3301 ccttttatat ctaaatacga taaaaaaatt cgtcaaatag ggggacaact tttcgttaat
     3361 ccagatgggc atgagtggtt acgtgaaaaa tggagtattc ctgtacgtcg ctattggaga
     3421 atttcagaaa gactaatggt taagtatgct aacttgctaa tttgtgatag taaaaatatt
     3481 gaaaagtata ttcaaaaaga ctatagtaaa tatgctccta aaacaaccta catcgcctat
     3541 gggactgatc tgacaaagtc aaaacacaat tttaaggaca aggttgttcg aagttggttt
     3601 gatgataaaa aaatttcaga aaataattat tatttagtag ttggtagatt tgtgcccgaa
     3661 aataattatg agtcaatgat tcgcgaattt atgaaatcta aatcaaaaag agatcttgtg
     3721 ttgattacta atattgagca aaatgttttt tatgagaaat taaaaaaaga aacaaaattt
     3781 gataaagata atcgaattaa atttgttgga acagtttaca atcagcaact tctgagctat
     3841 attcgtgaaa atgcctttgc ataccttcat ggtcatgaag ttggagggac taacccatcg
     3901 ttgttagaag cactggcttc aacgagtata aatttattat tagatgtcaa ttttaatcga
     3961 gaagttggag gaaatggtgc attatattgg aaaaaagata ctttaaacga agtaattaag
     4021 cgttgtgaac tgttgtctag agattctatt gagaagttag caaaacaatc gaaaaaacag
     4081 gtaagaaaat attttagttg ggacttaata atagatgctt atgagaagtt attctatgac
     4141 atagatagtt attttttagc aaaaggtaaa cattagactc taaaaatcaa taatttattt
     4201 ttttcttcat tgtcttttat ggggattgtt ttaggaggtt cccaagtttt caataatttt
     4261 gctattgata gttctctaaa gtatttatct tatttcatct tatttttatg tattattgtt
     4321 aaactcttaa gaaatccctt aaccataata tttagtagat tggtctattt tatatgtatt
     4381 tcattccttt ttataattgg aatctatttg caagatctac cattacatag gataatatat
     4441 ctttcatttt ctatgatcat aatttctggt ttggcaactt taacggtatc tttatttgat
     4501 aatgtaaaag atttaagaag agtatcttat tatatatttt atggagtctt attatcaact
     4561 atacttggaa ttttctatca agttcccatg tttactgttg cagttgaggg ggttggtgcc
     4621 acttctttag gttttaatgg gggaatgtta cacaaaaatt tctatgcgat aactattcta
     4681 acttcatata tactattatt tataagtagg aaatatggag taaggcataa tatagatagt
     4741 tatgtacttt ggtttgaatt cttccttata ttgatttcaa attcacgtac agtgtattta
     4801 attctaatca tattttattg tataaataat tggaagttat tattaagaat aaataaaaag
     4861 caaagaattt tagtttttat ccccgctata tttatttctt taattatagc gataatattt
     4921 tttgaatact taaccaataa tagcgactcc tatacttata gagttcaagg gataattaat
     4981 ttttttaatt attatcaatc ggactatttt cacttgcttt ttggtgatgc acagcttgct
     5041 tttggaggaa caacacagaa ttatacctat aatataagaa gtgtaattgg ttggaatgga
     5101 acagttgaga tgcctttatt aagtataatg ataaaaaatg gcttcattgg attgttagga
     5161 tatacagttg ttctactctc tttttttaaa aatataaaga aaataaaaaa tataaacata
     5221 aaaactgtag gattactagt gattatccca ttattgatgt cggcaactgt tgagaactat
     5281 atcgtaaatg taaattttat atttatgcca atttgttttt gtgttctaaa ctctcttaaa
     5341 agattagaac acaatttaaa cgtataagga tataacttta ttatgcaaaa tctagtaagt
     5401 attattctac ctgtttataa tgtagaaaat tatattgaaa attgtttaaa ctccattatt
     5461 tctcagagtt attttcattt tgaagtaatt attgtgaatg atggatcgac tgataatagt
     5521 atgagctact gtgaaaaaat agtatgcgaa gatttaaggt ttagaattat aaacaaagat
     5581 aatggtggtc tatctgatgc gcgtaatgta ggtttagaag cagcaaaagg ggactatatt
     5641 atttttgtag atagcgatga tttcatatct cctaacttag tttctcatct catggcatgt
     5701 ctggaaaatt atgaagcgga tcttgcaatt tgtgatccag ttcattttta tttgggaggt
     5761 aatcaagaga cacacgagaa aaatattttt aaaaaacatt caaacattca tttattaaca
     5821 aatgaagaag cgatttgtga acttttttat caaaaaacat ttttagtttc ggcttgggga
     5881 aaagtttata aaaagtctat ttttgataat attaggtttc caaaaggtaa gttatttgaa
     5941 gatagcgcag taatgcattt attatttgag aaaagcaata agattgttta ttcgaatgca
     6001 aaactgtacg catatgttca taggaaagat agtattacta ctagtcaatt ttctgaacgt
     6061 gatttagaca ttttagatat aactgatcaa attttgaagc gatacaagtc tttccctaaa
     6121 atttatcagg cagctatttc atataaaatt tctgcgtgtt ttagagttat cttgaatgct
     6181 ccaaacatag aaaaatatac tcccattgta gctgaatgta aaaaatatgt atattatcac
     6241 tggaaagata ttttatttaa taaaaaaact cgaataaaaa ctaaattagc attaatttca
     6301 attgttttat ttaaaccgtt tataaaattt atttatacac acgtcgatag atggaaatag
     6361 gtttataaaa taaaagaaag gttggtttat aaagcgttta tttctgagta ttctataaag
     6421 tagatgaggt aactaggaat atgaatttaa taatgtgtag aactcccctt cagactataa
     6481 ttgctgaacg gattattgag atgtttccca atgaatcatt tgaagttatt tgttattttg
     6541 agtcaagaaa taaaaaacaa gcatattatt caacaagatt aaaaaaaaaa agtgagctat
     6601 ataccgaaat agtatttaaa aatagggctc aggagatatt aactataata tactttcgtt
     6661 ttatatttct gaggaaatat tacgataaaa tctatattgc taacataaca aatgttttgt
     6721 ttcatacaat attatcagct tctactttta ataaaatttt tacttttgat gatggacttg
     6781 ctaatataat taaatctagt ttcttatatg ctagtaggac atctctaaaa gctaagtttt
     6841 ttaaatttgt ttttttgatt aagtatgatg cgcaaaggat aaaaaatgag tcatctttac
     6901 attatacctt atataaaaac aatgacaata ttataaaaaa cacaaagttt atttctattt
     6961 taagaaatat tactaaacat aaggatgtta gtaataattc tatatcaatt tgtataggtc
     7021 aaccactata tagcgatgat ttaatgaata tacattattt caataatatt attcataaat
     7081 atgatattga gtattatttc ccacatccac gggaaacctt taggattgac aatattattt
     7141 atattgagac acaatgtatt tttgaagaat atgttgtcaa tttacttaag gaattttctg
     7201 agattaatct ttatacttgt ttcagttccg cagcgttgaa tgtcatagat attgatggaa
     7261 tcaacgtttt tataataaag tcagcggaat ttgaagaaga gcaatcgatt tttaatgagt
     7321 ttaaacttac ttttgtatga tatgttcaaa aggagggagg atgaatacta agaaattact
     7381 tcaatctggt tttatttata caatcgggaa tttgttggtg caaggattag cctttatcac
     7441 acttcccatc tacactcggg tcatctcaac agaagtttat ggccaatata gtttatatat
     7501 cgcttggatg aatattatta tgctttttat tggccttcag acgagcggtt ctttaagttc
     7561 agcccgggtc aaatacggag aagaatttaa aagctattct gggagtgcct tttctgtagg
     7621 taatatatgg tttcttattg tacttttgat agcttttcta tttagaagtt ttcttgcacc
     7681 attagttggt ttttctgaat ctattttttt attaatggtg tgtcaaagtt acgctaacta
     7741 tgtggtgact ttctttggtc agtattttat acaacaacag aggagcttgg ctaatttaat
     7801 actatcctta gccaatgcag tttcatctgt tgcactatct ctatttttaa tttttcattg
     7861 gtccgatgac tttttatcta gggtttttgg agcttttgtt cctactataa taactggaat
     7921 agttgccttt gcttatattt attatcatag caaatctttt tacaatccta agtattttcg
     7981 gttcattgtc actgtgtctg ttcctttgat tttccatttg ttaggacacc agttgttggg
     8041 gcaattagat agaattatgc tagctcgttt atataatact aaagaagttg caatgtatag
     8101 tttcggttat tcgcttggaa tgattattca gattgttttg aatagtatta atatggcttg
     8161 gattccatgg ttttttgatg ctagaaaaga aaaattactg caactttcaa cttatattag
     8221 ccgttatctc tatttaggag ttttcttaac attaggttat ttgactgttt ttcctgagtt
     8281 agctcaaata atgggaggag acaagtatag tagtagtgtt caatttattt ctttaattat
     8341 tgtcagctac tttttagttt ttctctatac atttcctgta aatatccaat tcttctatgc
     8401 taatacgaca tggattccta ttgggacatt attagctgct ggtgtaaatt ggttactaaa
     8461 ccttgtatta attcctcatt atgcagccta tggcgctgct atggctacaa taatatctta
     8521 tttagccttg ttgatttttc atcatattgt ttcgaaagtt aaataccatt attcagatgt
     8581 ctcagttagg cagtacatca ttttaagtgg tatagtattt agttatgcta tgttaatgaa
     8641 catgttccta gataatatcg ttattagatg gagtttagga ataattattc ttatagtata
     8701 tagcattgtt tttcaaaaag tcattttaga cttattgagt aaaaaaagaa ggagaagata
     8761 atggtttata ttattgcaga gattggttgc aatcataatg gagatattaa tcttgcgaaa
     8821 aaaatggtag atgttgccgt gtcttgtggt gttgatgctg ttaaatttca gacttttaaa
     8881 gctgagaaac ttatttctaa atttgctccc aaagctgaat atcaaaaagc aactacagga
     8941 acagcagaca gtcaacttga gatgacgaaa cgtttagagt taagctttga agaatactta
     9001 gaaatgcgtg attatgcaat tttaaaaggt gtggagacct tttcaacacc ttttgatgaa
     9061 gagtcattag agttcttaat ttctacagat atgccaattt acaaaattcc atcaggagaa
     9121 atcactaatt taccttactt agaaaagatt ggcaagcaac aaaagaaagt tattctttcg
     9181 acgggtatgg cggtaatgga agagatccat caagcggtga atattttacg tcagaatggt
     9241 acaaccgaca tttctatttt acattgtaca acagagtacc caacacctta cccctctcta
     9301 aatttaaacg ttattcatac tttgaaagat gaatttaaag atttaacgat aggttattcg
     9361 gatcattcaa ttggatcaga agtacctatc gcagcagcag caatgggtgc agaagttatt
     9421 gaaaaacact ttactttaga tactaatatg gaaggtccgg atcataaagc cagtgcaaca
     9481 cctgatattt tagctgcttt agttaaaggg gttcgcattg ttgaacaagc cttaggtaga
     9541 tttgaaaaaa tcccagatcc agtagaagaa aaaaataaga ttgttgctcg taaatcagtc
     9601 gttgctttaa aaccaattaa aaaaggcgat atttattcaa tagaaaatat tacggtgaag
     9661 cgcccaggta atggtatttc tcctatgaac tggtatgaca tcttgggaca agaagcgcaa
     9721 gatgatttcg aagaggatga agttattcgt gattcacgct ttgaaaatca attgccctag
     9781 ttataataac acctaattat aatcggcaac agatgtatta cacggctaag attatgtaat
     9841 tactatatat agaagggata cgatgaaaaa aatttgttta gtaaccggat cgcgtgctga
     9901 atacggaata atgaagccat tgattcaaag attatcaaaa gataaagaag tcaacttaca
     9961 aattattgca acagcaatgc atctggaaga aaagtacggc tatacttatc gtcaaattga
    10021 agaagacggt tttgatattg cttataaagt tcccttacat ctttatgata ctgacagaag
    10081 aactgtatct actgcaatgg cgcatttaca actaggattg accaaaattt ttgacaagga
    10141 agactatgat ctagtcatca ttttagggga tcgttatgaa atgttaccag ttgtgaatgt
    10201 agcgttgatt tataatgtcc cagtatgcca ccttcatgga ggggagacat cattaggcaa
    10261 ttttgatgag tatattcgcc atgcaattac taagatgagt cacctacact tagtctctac
    10321 agaggacttt cgtcaacgtg tgattcagat gggagaacaa cctcaatttg taattaacac
    10381 aggagctctc ggagtggaaa atgctctatc aatcccttct ctaaccaaag aagcaataga
    10441 aaagcaatta ggtattgcac tagaggaatc ctattttgtt gttctctatc atcctgttac
    10501 ttttgaacaa ggaaaaagtg ctggagaaca gatgaaagcg gtactatcag ccctttctaa
    10561 atttggtgtc cagtgtctat ttataggatc caattcagat actggttctg atgatattgc
    10621 aaaggctatc aatacttatt tgataaatca tgaaaatagt tattgctttg cttctttatc
    10681 aacccaactt tatcattcct taattcgtca ttcactagga ctgattggta attcgtcttc
    10741 tggtttaata gaagttcctt ctttgatgaa acctactctt aatattggtg atcgccaaaa
    10801 aggtaggcta catggagaaa gtgtagtaag tgtaccagtc gaaacctcct ctgtattgga
    10861 gggattatca aaattgaatg aagtgaccaa ctttgacaat ccctactaca aagagaatgc
    10921 ttcatctatt gcatatgaag caataaagct gtatttgaaa gatgaacctt caatttccca
    10981 accattttat gatttaaagg agaataatct aaaatgaaaa aaaagttggc atttttaggc
    11041 gcaggaagtc atgctgatgc gattgctccg gttattgatc ctcttgtgta tgatttcgta
    11101 ggtttttttg atgataaaga tattacggag catgatggtt atcctgttct tggaaaactt
    11161 tatgatgtgc taccttacct tgaagatggc tcaatagatg cagtatttat tacaataggt
    11221 gataatgcta aaaggaaaga actatttgaa tatgtagcaa aggattatta tgactttatt
    11281 attaacatca ttagtcccaa tgctttagta ttgacaccag atagtatttg tggacgtggt
    11341 atctttattg gttttggggc ttttataggt tctaaagtga agctgtttga taacaacgtt
    11401 gttaatacag gagcgctcat tgaacatcat actgttgtag aatcacactg taatatagca
    11461 cctaatgcta ccataaatgg tctttgttat attagagaag aagtttatgt aggtagtgcc
    11521 agtgttatta ttcaaacctt ggatatttca tcgtgtacga caattggagc aggagctgtt
    11581 gttgtaaaag atattataga acctggaacc tatgtagggg ttccagcaaa aaaaataaaa
    11641 taaaggatat gttatgaagc caatttgtat tattcctgcg cgatcagggt caaaaggatt
    11701 accagataag aatatgttat tcctagcagg taaacccatg attttccata ctatagatgc
    11761 tgcaattgaa tctggaatgt ttgataagaa agatattttt gttagtacag attcagaatt
    11821 gtacagagag atttgtttag aacgcggtat ttcagtggtg atgagaaaac cggaactttc
    11881 aactgatcag gcaacttcgt atgatatgtt aaaagatttt ttatctgact atgaagataa
    11941 tcaggagttt gtgttacttc aagtaacttc tccactaaga aaatcatggc atataaagga
    12001 agcaatggag tattattctt cacatgatgt tgacaatgtt gtaagttttt ctgaagttga
    12061 gaaacaccct agtctgttta caacattatc tgatgaaggc tatgctatag atatggtggg
    12121 agcagataaa ggttatcgtc gccaagattt acaaccgtta tactatccga acggcgctat
    12181 ttttatttct aataaagaaa cttacttaag ggaaaaaagt tttttcacct ctaagacata
    12241 tgcttatcaa atggcaaagg aattttcatt agatgttgat acgagagatg attttatcca
    12301 cgtcatcggt cacctctttt tcgattatgc cattagggaa aaagagaata aagtttttta
    12361 taaagaaggc tatagtcgtt tgttcaatag agaagcttca aagataattt taggtgattc
    12421 aaaaacgata tctatctcac tagaaaatta ccataattat tcccaaggtg gtgtaacatt
    12481 agcaacgatg ttggaaaact tacctaactt tttgacagct aatgtaacag aggcttttgt
    12541 ttctataggg gtaaatgatc tcattaaagg ttatagtgtg tggaagatat attcagcaat
    12601 ttccaaaaac tctactcatt attagctgag aataaaa
//
