LOCUS       AB924553               16818 bp    DNA     linear   BCT 28-OCT-2015
DEFINITION  Klebsiella sp. 390 DNA, capsular polysaccharide synthesis gene
            cluster, serotype: K11.
ACCESSION   AB924553
VERSION     AB924553.1
KEYWORDS    .
SOURCE      Klebsiella sp. 390
  ORGANISM  Klebsiella sp. 390
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella.
REFERENCE   1
  AUTHORS   Pan,Y.J., Lin,T.L., Chen,C.T., Chen,Y.Y., Hsieh,P.F., Hsu,C.R.,
            Wu,M.C. and Wang,J.T.
  TITLE     Genetic analysis of capsular polysaccharide synthesis gene clusters
            in 79 capsular types of Klebsiella spp
  JOURNAL   Sci Rep 5, 15573 (2015)
   PUBMED   26493302
  REMARK    DOI:10.1038/srep15573
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16818)
  AUTHORS   Pan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2014) Contact:Yi-Jiun Pan National Taiwan
            University College of Medicine, Department of Microbiology; 1, Sec
            1, Jen-Ai Rd., Taipei 100, Taiwan
FEATURES             Location/Qualifiers
     source          1..16818
                     /organism="Klebsiella sp. 390"
                     /mol_type="genomic DNA"
                     /strain="390"
                     /serotype="K11"
                     /db_xref="taxon:1497796"
     gene            199..1101
                     /gene="galF"
     CDS             199..1101
                     /gene="galF"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose pyrophosphorylase"
                     /protein_id="BAT23278.1"
                     /translation="MKMNMANLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEIVLVTHSSKNAVENHFDTSYELEALLEQRVKRQLLAEVQAICPPGVT
                     IMNVRQAQPLGLGHSILCARPVVGDNPFVVVLPDIILDGGTADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPMVAEGQVARIVEFIEKPDEPQTLDSDLMAVGR
                     YVLSADIWAELERTEPGAWGRIQLTDAIAELAKKQSVDAMLMTGESYDCGKKMGYMQA
                     FVTYGMRNLKEGAKFRESIKKLLA"
     gene            1495..2124
                     /gene="cpsACP"
     CDS             1495..2124
                     /gene="cpsACP"
                     /note="orf2"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase homologue"
                     /protein_id="BAT23279.1"
                     /translation="MNWQLISFFGDSTVLLPSAAALFIVLMLRKTSRSLAWQWSLLFG
                     ITGAIVCASKLAFMGWGLGIRELDYTGFSGHSALSAAFWPIFLWLLSARFSVGLRKAA
                     IITGYVLAAVVGYSRLVIHAHSVSEVIAGLLLGAAGSALFLVLQKRTSDPENVNISWG
                     GVACLVMVPLILLHSGSKAPTQSLLGQIATAVGPLDKPFTRTDLHKQAW"
     gene            3003..4517
                     /gene="wzi"
     CDS             3003..4517
                     /gene="wzi"
                     /codon_start=1
                     /transl_table=11
                     /product="surface assembly of capsule"
                     /protein_id="BAT23280.1"
                     /translation="MPRALSILVFPSFSLRNDSGAENGSCTMIKIARIAVTLGLLSSL
                     GAQAYAAGLVVNDNDLRNDLAWLSDRGVIHLSLSTWPLSQEEIARALKKAKPSYSSEQ
                     VVLARINQRLSALKADFRVTGYTSTDQPGTPQGFGQTQPADNSLGLAFNNSGEWWDVH
                     LQGNVEGGERISNGSRFNANGAYGAVKFWNQWLSFGQVPQWWGPGYEGSLIRGDAMRP
                     MTGFLMQRAEQAAPETWWLRWVGPWQYQISASQMNQYTAVPHAKIIGGRFTFSPFQSL
                     ELGASRIMQWGGEGRPESFSSFWDGFTGHDNTGTDNEPGNQLAGFDFKFKLEPTLGWP
                     VSFYGQMIGEDESGYLPSANMFLGGVEGHHGWGKDAVNWYLEAHDTRTNMSRTGYSYT
                     HHIYKDGYYQQGYPLGDAMGGDGQLFAGKVELITEDNQRWSTRLVYAKVNPKDQSINK
                     AFPHADTLKGVQLGWSGDVYKTVRLNTSLWYTNADHSDSDDVGASAGIEIPFSL"
     gene            4667..5806
                     /gene="wza"
     CDS             4667..5806
                     /gene="wza"
                     /codon_start=1
                     /transl_table=11
                     /product="putative capsule polysaccharide export protein"
                     /protein_id="BAT23281.1"
                     /translation="MKKKIVRFSALALVIGFLSGCTIIPGQGLNSLRKNTIELPDSDY
                     DLDKLVNVYPMTPSLVDQLRPETLMARPNPQLDTLLKNYEYRIGVGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVQVAGKTLSQVRQEIAGRLTTYIESPQ
                     VDVSIAAFRSQKVYVTGEVTNSGKQAITNIPLTVMDAINAAGGLSADADWRNVVLTHN
                     GKDTKISLYALMQKGDLTQNHLLYPGDILFVPRNDDLKVFVMGEVGKQSTMKMDRSGM
                     TLTEAIGNAEGMSQAFSDATGVFVIRQLKGDKQGKIANIYQLNAQDASAMVLGTEFQL
                     QPYDIVYVTTAPLVRWNRVISQLVPTITGVHDMTETMRYIKRWPN"
     gene            5794..6240
                     /gene="wzb"
     CDS             5794..6240
                     /gene="wzb"
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="BAT23282.1"
                     /translation="MAQLMFDSILIVCTGNICRSPIGERFLRRELPNKKIDSAGTAAL
                     VGHDADESAIKVSVKHGLSLDGHKGRQFTASLGRQYDLILVMEKYHIEQIGRIAPEAR
                     GKTMLFGQWLNCREIPDPYRKSEEAFNSVYQLIEQAGLRWVEKLGA"
     gene            6258..8420
                     /gene="wzc"
     CDS             6258..8420
                     /gene="wzc"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="BAT23283.1"
                     /translation="MSSVKHKSVEKETDEIDLGRIVGEIIDNRKLIISIVSLFTLIAI
                     VYALFATPVYQADALVQVEEKQANAILSNLSQMLPDSQPQSAPEIALLQSRMILGKTV
                     DDLNLQAEVRQKYFPVFGSGWARLVGDKPGEIYVSRLYISMPNEDTPELTLKVIDNNN
                     YTISGDGFELSGKTGVLLDSKGLSIKIAKIDAKPGTEFTITYLTRLQAITELQKILTV
                     TDQGKDTGILSISLTGENKRLIENIVDSIIGNYLAQNISRQAAQDSKSLEFLSLQLPK
                     VRNELDLAEDKLNAYRRQNDSVDLSLEAKSVLDQIVNADNQLNELTFRESEISQLYTK
                     EHPTYKALMEKRKTLQDEKLKLNKKVSSMPETQQEILRLSRDVESGRAVYMQLLNRQQ
                     ELNIAKSSAIGNVRIIDEAVTQPKPVKPKKILVVLAGLILGGIISLGLVMIRMFLRRG
                     IETPEQLEDLGINVYASIPISDDFAKRVAQEQKWKRRKNNEVNGLLAIDNPADLAIEA
                     IRGLRTSLHFAMMEARNNVLMISGASPNAGKTFVSSNLAAVISQSGKRVLFIDVDMRK
                     GYTHKLFNVSNDNGLSDILSGKVDAKKAVRTIPTVEFDFIPRGMVPPNPAELLMHRRF
                     GDLVTWASANYDMVILDTPPILAVTDAAIIGNYAGTTLLIARFETNTAKEIEVSVKRL
                     EQSGVLVKGCILNGVVKKAGSYYGYGYNYYGYKYTDDK"
     gene            8690..10099
                     /gene="wcaJ"
     CDS             8690..10099
                     /gene="wcaJ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAT23284.1"
                     /translation="MDLIMNILPERYQISGNASIISMIQRLFDIVVVSSSLYIVYLIN
                     NIQYDLAFGVLILFALVVFQMIGGITDFYRSWRGNKLSLELLMIIKNWTLSLIILFLI
                     SNVYPIPNAEHFVFYQWYVIVCVGFFFFRCFIRYGIGVLRNLGYNRRTVAMVGAMPSG
                     IALFKSFQEQTWLGFVVKGIYDDENTDDLQGIPYAGDLNKLVEDAKSGKIDRVYIALA
                     IEDEDKIKHVLKELTDTTCSVLLIPDLFTFNILQSRTEEINGVPVVPLFDTPLNGINM
                     VFKRLEDIVVSTIILILISPVLLLISCVVKFTSPGPVFFRQIRYGMDGKPIKVWKFRS
                     MTVMENDGNVKQATKNDVRITKVGKVLRKTSLDELPQFFNVLFGQMSVVGPRPHAVSH
                     NEQYRALIQGYMLRHKVKPGITGLAQVNGWRGETDTLEKMEKRIEYDLLYIRNWSIWL
                     DLKIIFLTIFKGFVNKSAY"
     gene            10384..11433
                     /gene="wzy"
     CDS             10384..11433
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAT23285.1"
                     /translation="MLPYIIALLLVYSWVIAEYKILNRKAVLFPLLILVILATMRHYE
                     IGTDANVYTRYFRFPFNNYSFQFDPEVETGYQYLVYLVREIYSSDYSLYFFVMALVSI
                     FPVIYTLKSRSVDYPLSIYIYITFGLYMAMYNQIRQCIAMGICFLATRYLVNKNFIKY
                     FLVILIASQFHVTAYLMLIFYFVCHNKLRIEYKIIPIVLSSAIAAPLMIAHMALNNSR
                     YEHYTEEATKGKNGLLTVMLYVVIALFFYIIGKRLRKDNLEYRIYECMYLCGVALLLP
                     VAMLGTDPAGPQRIIQYFLYYVMLMFPIVFKKINNKFVYITFIILSFIYFVLMIASNI
                     AGVYPYQFNPVFDFF"
     gene            11461..12576
                     /gene="wcuF"
     CDS             11461..12576
                     /gene="wcuF"
                     /note="HG23; orf9"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAT23286.1"
                     /translation="MKVLHVAETFKGGVATVINSLATYQLNNKEIDGVEVIVPNEHIS
                     EISSVLKYNLTTFDRKKRGVVSLIKLGIVLYKKIKIYKPDIVHLHSTFAGFIGRILIL
                     SLFKNKNIKVVYCPHAFPFLMDSHKLKKTLYIKCEKILARFTSKIICVGDSEYNDASL
                     AGIPRDKMVVINNGVNIPTVSNQVNCNNCEEYVLLYVGRFDYQKGTDVFIDALKIIDN
                     TVLDYKIKVIMVGEPVNDSVDYKNQKFKNINIIFTGWINQESLFDYYLKANCLVIPSR
                     WEGLAMVPLEAISYKLPVIASNISAFQSINSLSGLSFTNEDPTSLAKLLSSLNGYDLN
                     KIKDILYYKVVTEYTQELMNEKTIVVYNNLLSQEILK"
     gene            12573..13454
                     /gene="wclO"
     CDS             12573..13454
                     /gene="wclO"
                     /note="HG109; orf10"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAT23287.1"
                     /translation="MKDNPLVTIYIPTYNRLELLKRALNSVVNQTYKNLEIIVVDDNS
                     SDGTQEFLKNFSKKDPRVSYILKTDNSGACVSRNLAIDKANGLFITGLDDDDYFSLNR
                     IEQLMDKSHLLNKYVFLYTYYLTVVAGGKYKKVRYLNSILPTTITGKELLHKNIIGNQ
                     CFTYTKRLRDAGKFSPDMPAWQDMELFYRLLTYSELNEAYLINMPLYFQDTSHDKNRI
                     TLSNKQKIYNAFEVFCKRNNIQNRDREILKAQLISYGIKIEYSSLFKRFIGNTNIYSC
                     LVDVFLIIKNMGNEKLV"
     gene            13467..14456
                     /gene="wcuC"
     CDS             13467..14456
                     /gene="wcuC"
                     /note="HG18; orf11"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyl hydrolase"
                     /protein_id="BAT23288.1"
                     /translation="MRKGIFMRKLLFVVFLFFSSVSYADDDTKILGVCFHPEKLNIDN
                     SKIFELLKKYHFVSYRTDYRWQYVERNKGEYKVPNDRLDKLIKESNNNNITPLLILGY
                     KNPLYSNDKPIDPATRSAFVNYVNWVVNRYKNYNVTYEIYNEWWSKDIKGYNLDRIRM
                     SANNYVELIKETSEKIRSISPDAKIIAGSLNPLDRRHVYWLEVMMQNGLMNYVDGISI
                     HPYSVNDPENDFKKIDDFQEEITKAFNNGKTVGVYITEMGYSNSSKGRINALEQEQYI
                     SKYIRLVNSRYYIKGIWWYQLINEKNTTEYESNLGLLNSDLSEKSIMKGFISR"
     gene            14840..16246
                     /gene="gnd"
     CDS             14840..16246
                     /gene="gnd"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate-6-phosphate dehydrogenase"
                     /protein_id="BAT23289.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIA
                     ENPGKKLVPYYTVQEFVESLETPRRILLMVKAGAGTDSAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILKQIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYALLKGGLALSNEELAQT
                     FTEWNEGELSSYLIDITKDIFTKKDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGNKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAQNAGIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAIAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWME"
BASE COUNT     5038 a   3078 c   3762 g   4940 t
ORIGIN
        1 ccgctgaata acctgaacga aaataagatt attctcactt ctgactgttg gtaaaaggga
       61 gttgtgtgta ttcttttacg tgatagtgaa tttaagaatt tggcgggcag atgcccgggc
      121 agtatccgtg tattatcagt ttcaatggat ttgtgacatg tacaattttg caaaaccgcg
      181 tgttttgcaa ccaaacaggt gaagatgaat atggcgaatt tgaaagcggt tattccggtc
      241 gcaggactag gcatgcatat gctgccggcc acaaaggcaa ttccaaagga gatgctgccg
      301 atcgttgata agccaatgat tcagtacatc gttgacgaaa tcgtcgccgc cgggattaaa
      361 gagattgttc tggtgacgca ctcctcgaag aacgcggtgg aaaaccactt cgacacctcc
      421 tacgagctgg aagccctgct ggaacagcgc gtgaagcgcc agctgctggc ggaagtgcag
      481 gccatttgcc cgccgggcgt gaccatcatg aacgtgcgtc aggcgcagcc gctgggcctg
      541 ggccactcca tcctctgcgc ccgcccggta gtcggcgaca acccgtttgt ggtggtcctg
      601 ccggatatca tccttgacgg cggcaccgct gacccgctgc gctataacct cgccgctatg
      661 atcgcccgct ttaacgagac cggtcgcagc caggtgctgg cgaaacgcat gccgggcgat
      721 ctctccgaat actccgtcat ccagaccaaa gagccgatgg tggctgaagg ccaggtggcg
      781 cgtatcgttg agtttattga aaaaccggac gaaccgcaga ccctggactc cgacctgatg
      841 gcggtgggcc gctatgtgct gtcggcggat atctgggccg aactggagcg taccgagccg
      901 ggcgcatggg gccgtatcca actgaccgac gccattgccg aactggcgaa gaagcagtcc
      961 gtcgacgcga tgctgatgac cggcgaaagc tacgactgcg ggaagaaaat gggctacatg
     1021 caggccttcg tcacctacgg gatgcgtaac ctgaaagaag gcgccaaatt ccgcgaaagc
     1081 attaaaaaac tgctggcctg agcgccggcg taagggggag cggcagggca ggggtatttc
     1141 gccacgccgc tcataacgtt atgcagagat tattcataac gtgaatgtta taacgattgt
     1201 aaagaaacgt atggcttcgg agacttctgg tagtttgtct ctggcctctg tggtgcaatg
     1261 gtcggagtag cggcaataat gctttatgaa tagtcactta gtgcgacaat tcctaaagcg
     1321 cagggaaagc ggtcaacgtc agccaaaaat aaaacttgac tgcgatcatg tttccgcttt
     1381 aaacatctca cttgcatgca taaaaatgtg aaatgaattg gcatgcgagc tggtacagac
     1441 attacggata atccctgagc acggcagtgg acatcatcga aggatcacag gtatatgaac
     1501 tggcaactca tctcattttt tggcgatagc accgttttgc tgcccagcgc agcggcgctg
     1561 tttattgtcc tgatgctgcg taaaacgtcg cggtcgctgg cgtggcagtg gagtttgctg
     1621 ttcggcatca ccggcgctat cgtctgcgct tccaaactgg cctttatggg ctggggtctg
     1681 ggtattcgtg aactggatta caccggcttc agcggccact ccgcgctctc cgcggccttc
     1741 tggcctatct tcctgtggct gctcagcgcc cgtttctccg tcggtctgcg aaaagcggcc
     1801 attattaccg gctatgttct ggccgccgtg gtgggctatt cgcggctggt catccatgcg
     1861 cattccgtct cggaagtgat tgccggcctg ctgctgggcg ctgctggcag cgctttgttc
     1921 ctggtgttgc aaaaacgtac ctctgatccg gagaacgtga atatctcatg gggcggtgtc
     1981 gcatgcctgg tgatggttcc gcttatcctt ttacatagcg gcagcaaagc gccgactcag
     2041 tccctgctgg gacaaatcgc caccgcggtg gggccgctgg ataaaccctt tacgcgtacc
     2101 gatctgcaca agcaggcctg gtaatcgcca tttgttttac aggggaaatt aagttttcgc
     2161 cggataatta gacaaataag aaaataatct actatatatc cggtaattga taattcatat
     2221 ttatgaaata atgaagtgct actacattgt tattgcatat ttgtctggat atagctttga
     2281 tcagacgttt cttaaatgcg ctgagacgtt acctgatgca cattcaatga atatccagac
     2341 actggaaact attagaaatt gtagttgact ataacgccat catctgattt ttgcatgatg
     2401 atgagctatg taaaaagacg tttttttaca gtcagttagc tgcatgtgtg atgatgtagc
     2461 agtaccggga agatctctaa ccctggaaat cagtgaataa tcttaattgg tgacccgctt
     2521 attttttgtc agtagaacga caggcggcat taattggcag atattcggta acaacactcc
     2581 caattgtgac cgaaatcccg taaacttaac gccgccaaaa ataatggcct gaccaattat
     2641 tcatccacag gtcgataaaa attaagtcgt tcaggtagtc agtgcgctgg tagctgttaa
     2701 gccaggggcg gtagcgtcgc tgaagccgct tgctgctgca cagaggttct cttcaacggc
     2761 tgtaaatatc gacccggtca ggatgatcat catcaataga tgtctaacga ttttctacgg
     2821 atatcctcag aagttggcca ggagcggctg ctgatatcgc ctagacgtta actttcctgt
     2881 ctcatccata gcctgtaggg taaaattcgc ctacattgtg agctgagtgg atgttataag
     2941 tctaacaagc agcttagggt aaatgtactt gcctcgccgg tgttgcacag cgaagtcagc
     3001 tggtgccgcg agcgctttct atcttggtat tcccctcatt ctcattgaga aacgacagcg
     3061 gggctgaaaa tggatcttgt acaatgataa aaattgcgcg cattgccgtg acgttgggtt
     3121 tgctttcctc cctgggagcc caggcttacg cggccgggtt agtagtaaat gacaacgact
     3181 tgcgtaacga cctggcctgg ctttccgatc gcggggtcat tcatctgagc ctgtcgacct
     3241 ggccgctgag ccaggaagag atcgcccggg cgttaaaaaa ggccaagccg tcttattctt
     3301 ctgagcaagt ggtgctggcc cgtatcaacc agcgactgtc tgctttaaaa gccgatttca
     3361 gggtcaccgg ctacacctca accgaccagc ctggcacccc gcaggggttt ggccagacgc
     3421 agccggcgga taattctctg ggcctggcat tcaacaacag cggcgagtgg tgggatgtcc
     3481 acctgcaggg caacgttgaa gggggagaac ggatcagcaa cggatcgcgc ttcaacgcca
     3541 acggcgccta cggcgcggtg aagttctgga accagtggct ctcgtttggc caggtaccac
     3601 agtggtgggg gcctggctat gaaggtagtc tgatccgcgg ggacgcgatg cggccgatga
     3661 ccggtttcct gatgcagcga gccgagcagg cggcgccgga aacatggtgg ctgcgctggg
     3721 tgggcccatg gcagtaccag atctccgcta gccagatgaa tcaatatacc gcggtgcctc
     3781 atgccaaaat tatcggcggt cgtttcactt tctcaccttt ccagtcactg gaattaggcg
     3841 cgtcgcgcat tatgcagtgg ggtggggaag ggcggcctga atcatttagc agtttctggg
     3901 atggcttcac tgggcatgat aataccggta ccgataatga gccgggtaac cagctggctg
     3961 ggtttgactt taaattcaag ctcgagccaa ctcttggttg gccggtaagc ttttatgggc
     4021 aaatgatcgg cgaggatgaa tctggttact taccatcagc caatatgttc ctcggcgggg
     4081 tcgaagggca ccacggctgg ggtaaagatg cggtgaactg gtatttggaa gcgcatgata
     4141 cacgtactaa tatgagtcga acaggctata gctatacaca tcatatttat aaagatggtt
     4201 attatcagca gggctatccg ctaggtgatg ctatgggggg agacgggcag ttgttcgccg
     4261 gtaaggttga actgataaca gaggataacc agcgttggag cacgcgtttg gtatatgcca
     4321 aggtaaatcc aaaagatcaa tcaattaata aagcattccc gcatgctgac accttgaaag
     4381 gtgtacaact tggctggagt ggggatgtct ataaaacagt ccgcctaaat acctcgctgt
     4441 ggtataccaa cgcagatcat agcgatagcg atgatgttgg cgcaagtgcc gggatagaaa
     4501 ttccttttag tttataggct gaatgcggca gattggtaaa gagattttgg taacgtagca
     4561 ggaagcaaat cgtatacatc tctatatcta accacacaag gatgtgctgg ataatcaata
     4621 gctgttcagg aaatggcaaa ttttagttta ctgatgatgt gacattatga agaaaaaaat
     4681 tgttagattt tccgcgttag ctttagttat aggcttttta tcgggttgta cgattattcc
     4741 tgggcaagga ttaaatagtt tacgaaagaa tacgattgaa ctccctgata gcgattacga
     4801 tctggataaa cttgttaatg tatatcctat gactccaagt ttggtagatc agttacgccc
     4861 ggaaacgctg atggctagac cgaatccgca actagatact ttgcttaaaa actacgagta
     4921 tcggattggg gttggggatg tactgatggt gacagtctgg gatcatccag aattgaccac
     4981 acctgctggt caatatcgga gtgctagtga tacgggaaac tgggtcaatt ctgacggaac
     5041 tattttctac ccttatattg gaaaggtcca agttgcaggc aagacactga gtcaggtgcg
     5101 acaggagatt gcgggccgct tgactactta catcgaaagt cctcaagttg atgtaagcat
     5161 agccgcattt cgctctcaga aggtttatgt cactggagaa gttaccaact caggaaagca
     5221 agcgataaca aatattccat tgacggtcat ggatgcgatt aatgccgcgg gtgggctatc
     5281 tgctgatgca gactggcgca atgttgtgct tacgcataat ggcaaagata ccaaaatttc
     5341 tctttatgcc ttaatgcaga agggggactt aacgcagaat catctgctat atccaggtga
     5401 tattctcttt gtgccccgta atgatgatct taaagttttc gtaatgggtg aggttggcaa
     5461 acagagcact atgaagatgg atcgtagtgg tatgaccctt actgaagcga taggtaatgc
     5521 tgaaggtatg tcacaggcat ttagtgacgc cactggtgta tttgttattc gccagctgaa
     5581 aggagataag caagggaaaa ttgctaatat ctaccaacta aacgcacaag atgcttcagc
     5641 aatggtgttg ggaactgagt tccagttgca gccttatgat attgtatacg ttactactgc
     5701 tcctttggtt cgttggaatc gggttatttc acagttggtt ccgacgatta ctggtgtaca
     5761 tgatatgaca gaaacaatgc gttatataaa gagatggccc aactaatgtt tgattcaatt
     5821 ctaattgtat gtactggaaa tatttgccga tcacccattg gtgaacgctt tctacgccgt
     5881 gaattgccta ataaaaaaat agattcagct ggtacagctg cattagtcgg acatgatgca
     5941 gatgaaagcg caatcaaagt ttcggttaag catggattat ccttagatgg gcataagggg
     6001 cggcaattca cagcctctct agggcgccag tatgatttaa tactagtcat ggaaaaatat
     6061 catattgaac aaattggccg cattgcaccc gaagccagag ggaaaactat gttgtttggt
     6121 caatggttga attgtagaga aattccagat ccgtatcgta agagtgaaga agcatttaac
     6181 tctgtatatc aattgatcga gcaagccggc ttgcgctggg ttgagaaatt aggcgcataa
     6241 atatcaggaa aaattgcatg tcatcagtta agcataaatc tgttgagaaa gagacagatg
     6301 aaatagattt aggacggatt gttggggaaa taattgataa tcgaaaatta attatttcga
     6361 tagtctcatt atttacattg atagcaatag tttatgcatt attcgctact cctgtatatc
     6421 aggcagatgc cttggtgcag gttgaggaaa aacaagcaaa cgctatattg agtaatttga
     6481 gtcaaatgct cccggatagt caacctcaat cggctccaga aatagctttg ttacaatcga
     6541 gaatgatttt aggcaaaacg gtagatgatc tcaatttaca agctgaagtc agacaaaagt
     6601 attttcctgt ctttggcagt ggttgggcta gactggtggg ggacaaacca ggtgagatat
     6661 atgtttcaag attatatatt tcgatgccta atgaagacac cccggaatta acattaaaag
     6721 taatagataa taacaattat acgatatctg gagacggatt tgagttatca ggtaaaacag
     6781 gtgttttatt agatagtaaa ggattatcaa taaagatagc taaaatagat gcaaaaccag
     6841 gtacggagtt cacaattacg tatctaactc gtttgcaagc tattacggaa ttacagaaaa
     6901 tactcacagt tacggatcaa ggtaaagaca ctggaatctt aagcatatca cttactggcg
     6961 aaaataaaag gcttattgaa aatatcgttg atagcataat tggaaattat ttagcacaga
     7021 atatttctcg gcaggctgct caggattcta aaagtctaga gtttttaagt ttacaattac
     7081 caaaggtccg gaatgagtta gatttggcag aggataaact taatgcctat cgtcgtcaaa
     7141 atgattctgt cgatctttca ttagaggcaa agtctgtttt agatcagata gttaatgctg
     7201 ataatcaatt gaatgaacta acttttcgtg aatcagaaat ctctcaactt tatactaagg
     7261 agcatccaac atataaggcg ttaatggaaa aaagaaaaac gcttcaagat gaaaaactaa
     7321 aacttaataa aaaagtttcg tcaatgccag aaacacaaca ggaaattttg cgtctgagtc
     7381 gtgacgtaga atccgggcgt gctgtatata tgcaattgtt aaatcgacaa caagagctta
     7441 atattgcaaa atctagtgca attggcaacg ttcgaattat tgatgaagcc gttacacaac
     7501 cgaaaccggt caaaccgaaa aaaatattag tagttttagc ggggcttatt cttggcggaa
     7561 tcatttcgct tggattagta atgattcgaa tgttcttgcg tagaggtatt gaaactcccg
     7621 agcagttaga ggatttaggt atcaatgtat acgcaagtat tcctatttca gatgactttg
     7681 caaagagagt ggcccaggaa caaaaatgga aaagaagaaa aaataatgaa gtcaatggac
     7741 tattagccat tgataaccct gcggaccttg caattgaggc gatacgaggg ctacgcacta
     7801 gtttgcattt tgcaatgatg gaagcaagga ataacgtgct catgatttcg ggggctagtc
     7861 cgaatgctgg taaaacattt gtgagttcaa accttgctgc cgttatatct caaagcggca
     7921 aaagagtttt atttattgat gttgatatgc gtaaaggtta tacgcataaa ttatttaatg
     7981 tcagtaatga taatgggctg tctgatatac tttcgggaaa ggttgatgct aaaaaggctg
     8041 taagaacgat tccgacggtt gaattcgatt ttattccgcg aggaatggta ccgcctaatc
     8101 cagcagaact actgatgcat cgacgcttcg gtgatttagt tacgtgggca agtgctaatt
     8161 atgatatggt tattttagat acaccaccaa ttctggctgt tacggatgct gcaataattg
     8221 gtaactatgc tggcactact ttgctaattg cacgtttcga gactaacact gccaaagaaa
     8281 tagaggtaag tgttaagcga ttagagcaga gtggtgtatt agtgaaagga tgtattttga
     8341 atggagtagt aaagaaagcc ggtagctatt atggatacgg atataactat tatggctaca
     8401 agtatactga tgataaataa atgatgatct tggcactgaa gaaagtctct tcagtgccaa
     8461 gataatacta tatcgttttg tgtgaaaaga gagtgtgtca tttaatacac aaaaaattca
     8521 tattgctagg atatttaaaa ttatacatga atcgacaaac cttactgtta aattgcagag
     8581 tgtaataaaa tgcaacgtct tattagagta gagtttgaag ttatataacg atatatgttg
     8641 taatatttat catcagtgat ttctacttta tattcaattc tatgacctaa tggatttaat
     8701 aatgaatatt cttcctgaaa ggtatcaaat ctctggtaat gcttcaatta tatcaatgat
     8761 acagagattg tttgatattg tagttgtttc ttcatcacta tatatagttt atctaattaa
     8821 taatatacaa tatgatttag cattcggagt gttaatatta ttcgctcttg ttgtttttca
     8881 aatgattggt gggatcactg atttttatcg ttcatggagg ggaaataaac tctcacttga
     8941 attgttaatg ataataaaaa actggacttt aagcttaata atcctttttt taatctcaaa
     9001 tgtatatcct atacctaatg ctgagcattt tgtattttat caatggtatg tcattgtatg
     9061 tgttggtttt ttttttttta ggtgcttcat tagatatgga attggtgtac taagaaatct
     9121 tggttataac cgtcgcactg tagctatggt aggtgctatg ccaagtggta ttgcattatt
     9181 caaaagtttt caggaacaga cgtggcttgg atttgtagtc aaaggtattt acgatgacga
     9241 gaatacagat gatttacaag gaataccata tgctggcgat ttaaataaac ttgttgagga
     9301 tgcaaaatct ggaaagatcg atagagttta tatcgctctt gctatcgaag atgaagataa
     9361 gataaagcat gttcttaaag aattaactga tacaacatgc tcagttcttt taattcctga
     9421 cctatttaca ttcaatatat tacaatctcg tacagaggag attaatggtg tccctgttgt
     9481 acctttattt gatacccctc ttaatgggat caatatggta ttcaagagat tagaagatat
     9541 agtggtttcg acaattattc tgatcttaat ttctcctgtt ttgttactta tttcttgtgt
     9601 tgttaagttt acctcacctg gtcccgtctt ttttcgacag atacgttatg ggatggatgg
     9661 caaaccaatc aaagtttgga agttccgctc aatgacggtt atggaaaatg atggtaacgt
     9721 taagcaagcg acaaaaaacg atgttaggat tacgaaggta ggcaaggtct tgcgtaagac
     9781 ctctttagat gagctaccgc aattttttaa tgtacttttt gggcagatgt cagtagtagg
     9841 gccaagacca catgcagtct ctcataatga gcaatataga gcacttattc aaggatacat
     9901 gctcaggcat aaagttaaac caggcattac ggggttggcg caggttaatg gttggcgcgg
     9961 cgaaactgat acactagaaa aaatggaaaa aagaattgaa tatgatttac tttatatccg
    10021 taactggagt atatggttag atttaaaaat aatttttctt acgattttta aaggatttgt
    10081 taacaaatca gcatactaag ctttatcata caaagagcag gagaaaatca aatctttgat
    10141 atctcctgtt cgctacttgt aataagtata aatactgtat attagttaca ctacaacacg
    10201 caatgtattt tctctcactg gtatgtttta taaataaata tatgcatgtt gttattattt
    10261 ttatcttatc tgcgctgaga tatatataca aaatagactt cgtaatctta attacctata
    10321 atagaaaata tcgtataatg gataaaagga attaagaaaa atttaatttt tacgagataa
    10381 aaaatgctcc catatattat tgcactatta cttgtctatt catgggttat tgctgaatat
    10441 aagatattaa atagaaaggc cgtcctgttc ccattgctta tattggtgat attagccacg
    10501 atgagacatt atgagattgg gaccgatgcg aatgtttata ctcgttattt tcgttttcct
    10561 ttcaataatt attcatttca atttgatcca gaagtagaga ctggatatca atatttggta
    10621 tatttagtac gtgaaattta tagtagcgat tattccttat atttttttgt gatggcgcta
    10681 gtttctattt ttccagttat atatacttta aaatcgcgat cagtagatta tcctctctca
    10741 atatatattt atatcacatt tggtttgtat atggcgatgt ataatcaaat tcgccaatgt
    10801 attgcaatgg gaatctgttt tttggcaaca aggtatttag tgaataagaa ttttattaaa
    10861 tatttccttg tgattctaat cgcctctcaa tttcatgtga cagcatatct tatgctgata
    10921 ttttatttcg tctgtcataa taaactacgg atagaatata aaataatacc tatagtttta
    10981 agtagtgcca tagccgcacc acttatgata gctcatatgg ctttgaacaa ctcaagatat
    11041 gagcattaca cagaggaggc aacaaaaggc aaaaatgggt tgttaaccgt tatgctctat
    11101 gtagtgattg cgttattctt ttatataata ggaaaacggt taaggaaaga taatttagaa
    11161 tatagaatct atgagtgcat gtatctatgt ggagttgctt tattactccc ggttgctatg
    11221 ctagggacag atcctgcagg tcctcaacga attattcaat attttttata ctatgtcatg
    11281 ttgatgttcc ctatagtatt caaaaaaata aataataaat ttgtatatat tacattcatt
    11341 attctatctt ttatatattt tgttctgatg attgcaagca atattgctgg tgtttatcca
    11401 tatcaattca atccagtgtt tgattttttt tgacaataga taactattga ggttatataa
    11461 atgaaagtac tacatgtagc agaaaccttt aaaggaggtg tcgctaccgt tattaatagc
    11521 ttggccacat accagttaaa taataaagaa atagatggtg tagaagtcat tgttcctaat
    11581 gagcatattt cggaaatatc gtcagtatta aagtataatc tgacaacatt tgatagaaaa
    11641 aagagaggcg tagtatcatt aataaaactt gggattgttt tatataaaaa gataaaaata
    11701 tataaacctg atatagttca tcttcatagc acttttgctg gatttattgg gcgtatacta
    11761 atactttctc tgtttaaaaa taaaaatatt aaagtagtct attgtccaca tgcttttcca
    11821 tttctaatgg attcacataa attaaagaaa acattataca ttaaatgcga aaaaatatta
    11881 gcaaggttca caagtaaaat tatttgtgtt ggtgatagtg aatataacga tgcttctcta
    11941 gcaggtattc ctagagataa aatggttgtg atcaataatg gtgtgaatat tccgacagta
    12001 tcaaaccaag ttaattgtaa taattgtgaa gaatatgttt tgttatatgt tggccgtttc
    12061 gattatcaga aaggaactga tgttttcata gatgcactga aaattataga taatacagta
    12121 ctcgattata aaattaaagt tattatggtt ggcgaaccag taaatgatag tgttgattat
    12181 aaaaatcaga aatttaaaaa tatcaatata atatttacag gttggattaa tcaagaatca
    12241 ttatttgatt attacctaaa ggcgaactgt ttagttattc cttctcgttg ggaagggctc
    12301 gctatggtac ctcttgaagc aataagttat aaattaccag ttatcgcaag taatataagt
    12361 gcctttcaat caataaatag tttaagtggt ctctcattta caaatgaaga tcctacttca
    12421 cttgcaaaat tattatctag tttaaatggt tatgatttaa acaaaataaa agatattctg
    12481 tattacaagg ttgtaaccga atatacacaa gaattaatga atgaaaaaac tatcgtagta
    12541 tataataatt tactttctca agagatattg aaatgaaaga taatccgtta gtcacgatct
    12601 atattccaac atataatcgt ttggaattat tgaaacgcgc acttaattca gttgttaatc
    12661 agacgtataa gaatttggaa attattgttg ttgatgataa ttcaagcgac ggtacacaag
    12721 agttccttaa gaactttagt aaaaaagatc cacgagtaag ttatatttta aaaacagata
    12781 attctggcgc ttgtgtaagt cgaaacttag cgatagataa agcaaatgga ttgtttataa
    12841 ctggattaga tgatgatgat tatttctcac taaacaggat cgagcaattg atggataaat
    12901 cacatctttt gaacaagtat gtgtttttat atacatatta cctaactgtt gtggctggag
    12961 ggaagtataa aaaggttaga tatctaaatt caattttgcc aacaacaata acaggaaagg
    13021 aactgttaca taaaaatatc attggcaacc aatgttttac atatacaaaa aggttgagag
    13081 atgccgggaa gttttctcct gacatgcctg cgtggcaaga tatggagctt ttctatagat
    13141 tgcttacgta ttctgaacta aatgaagcat atttgattaa tatgccttta tatttccaag
    13201 atacctcaca tgataaaaat agaataacgc ttagtaataa gcagaaaata tataatgcgt
    13261 ttgaggtttt ctgcaaaagg aacaatattc aaaataggga ccgagaaata cttaaggctc
    13321 agttaattag ttatggcatt aaaatagaat attcttcact attcaaacgt tttataggca
    13381 ataccaatat ttattcatgt ttggtggatg tgttcctgat tataaaaaat atgggtaatg
    13441 aaaaactagt ttgatcggca tattagatga ggaaaggtat atttatgcgg aaacttctat
    13501 ttgttgtgtt tttatttttc tcatccgttt cgtatgcaga cgatgacaca aaaattcttg
    13561 gagtgtgctt tcatcccgaa aagttaaaca tagataactc taaaattttt gagttgctca
    13621 aaaaatatca tttcgtttca tatcgcacag attatagatg gcagtatgtg gaaaggaaca
    13681 agggagaata taaagtacct aatgacagat tagataagtt gattaaagaa tcaaataata
    13741 ataacattac gcctttactg atattaggat ataagaatcc actatatagt aatgataaac
    13801 cgatcgaccc tgcgacaaga agtgcgttcg tcaactatgt caattgggtt gtgaatagat
    13861 acaaaaatta taatgttact tatgagattt ataatgaatg gtggtcgaaa gatattaagg
    13921 gttataattt agaccgaata aggatgtcag caaataatta tgttgaattg ataaaggaaa
    13981 caagtgaaaa aattcgttct atttctccag atgctaaaat tatagctggt agtttaaatc
    14041 ctttagaccg tcgtcatgta tattggcttg aagtgatgat gcaaaacggc ttaatgaact
    14101 acgtggatgg catatctata cacccctact ctgtaaatga tcctgaaaat gattttaaga
    14161 agatagatga ttttcaagaa gaaatcacca aagcatttaa taatggaaaa acagtaggcg
    14221 tttatatcac ggaaatgggg tattcgaact ctagcaaagg aagaattaat gcattagagc
    14281 aagagcaata tatttctaaa tatattcgtc ttgttaattc aagatattat atcaaaggga
    14341 tatggtggta tcaattgatc aatgaaaaaa atactacaga atatgaatcc aatttaggat
    14401 tgctcaattc ggatttatca gaaaaaagta taatgaaagg tttcatctcg aggtagctat
    14461 tttgattatt ttttaacgtc ctgattttga aagttatcgt gtgacttgtt acgtgcaaag
    14521 tatctcttgg taattactta aatacattat attgtgacgc gtgcttacaa gaaattactt
    14581 tgacttataa aaaattaacg attcttatgg tttaggactt ttattgagat gcacttctga
    14641 ttcaaggatg cagaacaaag ctggcagatg gaaatgttaa gctcaatatt gtcaggcgta
    14701 tttatggcca ttttatttcc tcatatgata aatcataaaa ataaatttac cctatgctct
    14761 taaccatctt tgagctaaca taataactgt ttttaagctg cgagcgtgtc gcagtgacca
    14821 caccagacag gagtatgtaa tgtccaagca acagatcggg gttgtcggta tggcagtaat
    14881 ggggcgcaac ctagcgctca acatcgaaag ccgtggttat accgtctcag ttttcaaccg
    14941 ctcccgtgaa aagaccgaag aagttattgc cgaaaatcca ggcaagaagc tggttcctta
    15001 ctatacagta caagagtttg ttgaatccct cgaaacacca cgccgtatcc tgttgatggt
    15061 gaaggccggg gctggcaccg acagcgccat cgattccctg aagccttacc tcgataaagg
    15121 cgacatcatc attgatggcg gcaacacctt cttccaggac accatccgtc gtaaccgtga
    15181 gctgtctgcc gaaggtttta actttatcgg taccggtgtt tccggtggtg aagagggggc
    15241 tctgaaaggg ccttccatca tgcctggtgg gcagaaagaa gcctatgagt tagttgctcc
    15301 gattctgaag cagattgctg cggttgctga agacggtgag ccatgtgtga cctatatcgg
    15361 tgctgacggt gctggccatt atgtgaagat ggttcataac ggcatcgagt atggagacat
    15421 gcagctgatt gctgaagctt atgcgctgtt gaaaggcggt ctggctcttt caaacgaaga
    15481 gctggcgcaa accttcaccg aatggaacga aggtgaactg agcagttacc tgatcgacat
    15541 taccaaagat atcttcacca agaaggatga agaaggtaaa tacctcgttg atgttattct
    15601 tgatgaagct gcaaacaaag gtaccggcaa gtggaccagc cagagctcac tggatctcgg
    15661 cgaacctctg tctctgatca ccgagtctgt atttgctcgt tacatctctt cgcttaaaga
    15721 ccagcgtgta gctgcttcga aagtgctgag tggtccgcag gctcaaccgg ccggcaataa
    15781 agcggaattt atcgaaaaag ttcgtcgcgc gttgtacctc ggtaaaatcg tctcctatgc
    15841 tcagggcttc tcccagctgc gtgctgcttc tgatgaatac aactgggatc ttaactacgg
    15901 cgagatcgct aagattttcc gcgctggctg catcattcgt gcgcagttcc tgcaaaagat
    15961 caccgatgct tatgcgcaaa acgctggcat tgctaacctc ctgctggcgc cgtacttcaa
    16021 acagatcgct gatgactatc agcaggcgct gcgtgatgtc gtggcgtatg ccgtacagaa
    16081 tggtattccg gtaccgacgt tctctgccgc aattgcctac tacgacagct accgttccgc
    16141 agttctgccc gctaatctga ttcaggcgca gcgtgactat ttcggtgctc atacctataa
    16201 gcgcactgat aaagaaggcg tattccatac tgagtggatg gaataatttt ctacaaagac
    16261 aatcaaatcc cggttatcta ctaaccggga tttttctata aaactctctc tatttaaata
    16321 ctttctgtta agaatgttta tcctcctatt agagttctga tgcttgttct ctaccatccc
    16381 atgtcagttt cttgacatgt actaagacag agagtaaaac cgccacaagt gttgattaca
    16441 gggtggttac taagccgccg tccagttaac tgattaaagt gtgatcgaat gaaaattact
    16501 atttccggta caggttatgt tggtttatcg aacggtgtcc tgattgcgca aaaccacgaa
    16561 gtggtggccc tggatatcgt acaggccaaa gtggatatgc ttaaccagaa gatctctccg
    16621 attgtcgata aagagattca ggaatatctg gcagaaaaac cgttaaattt ccgtgccacc
    16681 acggataagc acgacgcata ccgtaatgcc gactacgtga ttattgccac gccgaccgac
    16741 tacgatccca aaaccaacta cttcaacacc tctacggtgg aagcggttat tcgcgatgtc
    16801 acaaagatca acccgaac
//
