LOCUS       EU038215               11684 bp    DNA     linear   BCT 11-JUN-2008
DEFINITION  Neisseria meningitidis strain 2004-038 CtrD (ctrD), CtrC (ctrC),
            CtrB (ctrB), CtrA (ctrA), GlcNAc-6-P epimerase (siaA), CMP-NeuNAc
            synthetase (siaB), and Neu-NAC synthetase (siaC) genes, complete
            cds; insertion sequence IS1301 unknown genes; and
            polysialyltransferase (siaD) gene, complete cds.
ACCESSION   EU038215
VERSION     EU038215.1
KEYWORDS    .
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11684)
  AUTHORS   Tsang,R.S., Tsai,C.M., Henderson,A.M., Tyler,S., Law,D.K.,
            Zollinger,W. and Jamieson,F.
  TITLE     Immunochemical studies and genetic background of two Neisseria
            meningitidis isolates expressing unusual capsule polysaccharide
            antigens with specificities of both serogroup Y and W135
  JOURNAL   Can. J. Microbiol. 54 (3), 229-234 (2008)
   PUBMED   18388994
REFERENCE   2  (bases 1 to 11684)
  AUTHORS   Tsang,R., Tsai,C.M., Henderson,A., Tyler,S., Law,D., Zollinger,W.
            and Jamieson,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2007) Public Health Agency of Canada, National
            Microbiology Laboratory, 1015 Arlington St., Winnipeg, Manitoba R3E
            3R2, Canada
FEATURES             Location/Qualifiers
     source          1..11684
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /strain="2004-038"
                     /db_xref="taxon:487"
     gene            complement(1..651)
                     /gene="ctrD"
     CDS             complement(1..651)
                     /gene="ctrD"
                     /codon_start=1
                     /transl_table=11
                     /product="CtrD"
                     /protein_id="ABW08122.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(648..1445)
                     /gene="ctrC"
     CDS             complement(648..1445)
                     /gene="ctrC"
                     /codon_start=1
                     /transl_table=11
                     /product="CtrC"
                     /protein_id="ABW08123.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(1445..2608)
                     /gene="ctrB"
     CDS             complement(1445..2608)
                     /gene="ctrB"
                     /codon_start=1
                     /transl_table=11
                     /product="CtrB"
                     /protein_id="ABW08124.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(2623..3798)
                     /gene="ctrA"
     CDS             complement(2623..3798)
                     /gene="ctrA"
                     /codon_start=1
                     /transl_table=11
                     /product="CtrA"
                     /protein_id="ABW08125.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            3933..5066
                     /gene="siaA"
     CDS             3933..5066
                     /gene="siaA"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcNAc-6-P epimerase"
                     /protein_id="ABW08126.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTYKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGAAVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            5070..5756
                     /gene="siaB"
     CDS             5070..5756
                     /gene="siaB"
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-NeuNAc synthetase"
                     /protein_id="ABW08127.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            5757..6815
                     /gene="siaC"
     CDS             5757..6815
                     /gene="siaC"
                     /codon_start=1
                     /transl_table=11
                     /product="Neu-NAC synthetase"
                     /protein_id="ABW08128.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIRACP"
     mobile_element  6802..7644
                     /mobile_element_type="insertion sequence:IS1301"
     CDS             6883..7338
                     /note="ORF1"
                     /codon_start=1
                     /transl_table=11
                     /product="unknown"
                     /protein_id="ABW08130.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQNFFLIYEKKLTKNGYSSTEVMYGVINMQV
                     ELGVVSIEQLDKAVAETRQKYTYVWTRMEIRSILKSLGVTCTTVKLQTT"
     CDS             7095..7634
                     /note="ORF2"
                     /codon_start=1
                     /transl_table=11
                     /product="unknown"
                     /protein_id="ABW08131.1"
                     /translation="MAEFFFDLRKEIDKEWVFIDGSYVRCHQHASGARRGFDRAIGQS
                     RGGNTTKIHLCVDSHGNPLDFKVTGGNVHDGQVANDLIEVIQEAQYFIADKGYDSQEI
                     RDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLKQFRSIATRYEKLA
                     RNFKSMLYLACSIIHAKLN"
     gene            7803..10916
                     /gene="siaD"
     CDS             7803..10916
                     /gene="siaD"
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase"
                     /protein_id="ABW08129.1"
                     /translation="MAVIIFVNGIRAVNGLVKSSINTANAFAEEGLDVHLINFVGNIT
                     GAEHLSPPFHLHPNVKTSSIIDLFNDIPENVSCRNIPFYSIHQQFFKAEYSAHYKHVL
                     MKIESLLSEEDSIIFTHPLQLEMYRLANNNIKSKAKLIVQIHGNYMEEIHNYEILARN
                     IDYVDYLQTVSDEMLEEMHSHFKIKKDKLVFIPNITYPISLEKKEADFFIKDNEDIDN
                     AQKFKRISIVGSIQPRKNQLDAIKIINKIKNENYILQIYGKSINKDYFELIKKYIKDN
                     KLQNRILFKGESSEQEIYENTDILIMTSQSEGFSYIFLEGMVYDIPILAYNFKYGAND
                     FSNYNENASVFKTGDISGMAKKIIELLNNPEKYKELVQYNHNRFLKEYAKDVVMAKYF
                     TILPRSFNNVSLSSAFSRKELDEFQNITFSIEDSNDLAHIWNFELTNPAQNMNFFALV
                     GKRKFPMDAHIQGTQCTIKIAHKKTGNLLSLLLKKRNQLNLSRGYTLIAEDNSYEKYI
                     GAISNKGNFEIIANKKNSLVTINKSTLELHEIPHELHQNKLLIALPNMQTPLKITDDN
                     LIPIQASIKLEKIGNTYYPCFLPSGIFNNICLDYGEESKIINFSKYSYKYIYDSIRHI
                     EQHTDISDIIVCNVYSWELIRASVIESLMEFTGKWEKHFQTSPKIDYRFDHEGKRSMD
                     DVFSEETFIMEFPRKNGIDKKTAAFQNIPNSIVMEYPQTNGYSMRSHSLKSNVVAAKH
                     FLEKLNKIKVDIKFKKHDLANIKKMNRIIYEHLGININIEAFLKPRLEKFKREEKYFH
                     DFFKRNNFKEVIFPSTYWNPGIICAAHKQGIKVSDIQYAAITPYHPAYFKSPKSHYVA
                     DKLFLWSEYWNHELLPNPTREIGSGAAYWYALDDVRFSEKLNYDYIFLSQSRISSRLL
                     SFAIEFALKNPQLQLLFSKHPDENIDLKNRIIPDNLIISTESSIQGINESRVAVGVYS
                     TSLFEALACGKQTFVVKYPGYEIMSNEIDSGLFFAVETPEEMLEKTSPNWVAVADIEN
                     QFFGQEK"
ORIGIN      
        1 ttaaggcagc gaattataat attcgtaggc tttgtccata tcttcaaact ggtacatatg
       61 ccctttttcc agcaccatcg cattatcgca atattgcttc atggcgctgt ggctgtgcga
      121 caccaagatg atggaacggt ctttgcgctt ttcaaacaac tcgtacttac atttatcggc
      181 aaaacgcgag tcaccaactg caatcacttc gtcaatcaaa tagcaatcaa actctaccgc
      241 caacgacaaa gcaaatgcca aacgcgcctt catacctgaa gaataacgct tgaccggctc
      301 gtaaagatat tgccccaact cggaaaactc ttcggtaaag ttcttcacat aatcgatatc
      361 gacattgtaa atccggcaga tgaaacgcaa attgtccata ccggtcagac tgccttgaaa
      421 cgcaccggag aatgccaaag gccaagaaat actcattgtc cgcttgattt cacccgtggt
      481 cggcggctca acgccactga tcaaacggat gagcgtcgat ttacctgcac cgttgcggcc
      541 gagaataccg attttctcgc ccttctccat tttgaagcta atatcgtgca agactgtccg
      601 ccaaccttgg cgggtcagat agcgtttgga aacgtgttca actgaaatca ttgcggctcg
      661 actcctttac tgaatttact gaccatcgcc aagccaaaca acaacaacac cagattgcac
      721 aatacgatat accaaggatt ttcataggta attacatcgc tgccaaaata tccggcacgg
      781 aacatttctg tgccatgcac catcgggatc atcaatgcat actcttgcac tttcggaggc
      841 aaattatgta caaaaaagaa tgcacccgat aacggcatca tcacaaaagt caatgtgccc
      901 caaatcttgc caaacggctc gaaattaaag gcaatcgaac aaatcaccaa acccaaacca
      961 atcgcaaaaa aagccatcaa aagccaagcc atcagcatat aaaacatatc tgccggcatt
     1021 tcaatccagc caatcgcaat caataccgcc ataatcacaa tctgcgcaat ggttgcacca
     1081 gcaatttcca aaatcatgcg cgccaagatg gtatccaaaa ctcttacatt gcggtgataa
     1141 agcaagctgg catttgaaga aatcgaccca actgcccgtt ttgaggcatt acgccacatc
     1201 atcaacatcg gatagccagt aatcgcaaat gcgacaatat tcaaagttga atatcggtct
     1261 gcctttaaaa atttccacat caagacgata acgaatgtca tcagcaacgg ctcaacaaac
     1321 agccataaaa agccaatatt attgcgaccg taacgggtga taatttcccg catcaaaagc
     1381 gcaccgatta cgcgcctttg aatggctaaa gattcccaaa atgatgtttt atgcaaggct
     1441 ttcatcagtt tttatgctca cgaatgctgg cagtcaacag gctcaaaata ccataaacca
     1501 tcaagccgat aatcagagtg gcaacaatgt tgtataaccg tttaggctca tgtgccaaat
     1561 ccggcaggct cggttgcgag atcacttcca aataaagctg ctgacggtct gcttcaacct
     1621 tggcactttc caaagaagtc atggcggctg ccaactgctg ctctgccaac tggttttcca
     1681 aatacacacg ctgatattcg gcagcctgat tagacaacga agaatgccca ccgcccgaaa
     1741 tggcacgtaa ctgttggtca atttctttac gcaagctctg ctcacgcgcc tgcaaacccg
     1801 gaatctgcgg attctccgga gtgactgctt tcacctgatc cagctgggtt tgaatcacaa
     1861 tcaattcatc ttgcagcttg gaaaccaacc ccatttgcac ttccgattgc gctttcaaat
     1921 caaaaacgcc attggcaatc cggtaatccg tcagattctg agaggcttcc tttacccgct
     1981 ctgccgccgt tttcactact tcttccgcat agcgcaccgt atcagcacgt gcacgatcgt
     2041 tcaactggtt aatcaatgct tcaccttgtt ttaacaaagc ctcattgatt ttcttagatt
     2101 ccagcgcatc aaaggaagtt acattcaacg tggaaatacc cgaaaccgta tcaaaattga
     2161 tcatcacctg atttttatag tattgataaa aagcctcttc ctcgccacgg aacccaaacc
     2221 cattaaagcg gctgaacgca tcacctttgg tttcataaaa ctcacgcacc ggcaagattt
     2281 tacgcagttc atccaaagac gagcgcgaac gcatatactc cccaaccgtg taaatatcat
     2341 cttgcgcacg ggcaaaacct gtgccctgca aaatggcacc caggccattg agagaagatt
     2401 ggcttttagg cgagcgcacc acaaagctcg attgcgacgt aaaacgatcg gaagcgaaga
     2461 agccgaaata caccaacgaa attaccgtag ggataatcac cgttacccaa aataaagggc
     2521 ttagcttttt aatccaactt ttctttttcg gctttttacg ctcggctttg gtttcagtgg
     2581 caactgccac aggaagttgc tcagacatct taattactca cattaattag ttaaattatt
     2641 aatactgttc gcgccactgg taaccggcga gaacacaaac gacaaaaatt tctgcacttc
     2701 agccaacggc gcattcgaca catacaatac atctttattc ttcacaggaa agcgctgcat
     2761 agaaaatagc gaatgcgcat cagccatatt cacacgatat accgttggaa tctctgcctc
     2821 actgccataa ccttgagcaa tccatttatc ctgacgttct gccggcaatt ccaccaatgg
     2881 cgtatagcgg aacacaaaca caccacgcgc atcagaacgg cgatcttgca aaccgcccat
     2941 acggccaatg gcttcagaaa gcgataagcc tctggctgaa aaaccgattt cttgtgttct
     3001 ccccaccgca cccatagacg taaaggtata gggattggta atcatggtaa ccacatcacc
     3061 gcgacgcagc aaaatatttt gtcgcggatt tgcaactaaa tcttccaagg caacagttcg
     3121 tactacattg ccacgtgtca gctgcacatt cgtatcctgc acatttgccg ttgaaccacc
     3181 taccgcagcc accgcatcca acacacgctc accggctgcc gtcagcggca tacgcacact
     3241 attcccagca cgaatcaccg acacattcgc cgcattattc tgcaccaaac gcaccatcac
     3301 ttgtggctga ttggccattt ttttcaggcg gcctttaata atttcctgaa cctgaccagg
     3361 cgttttaccg accaccgaaa tatcgccaac aaacggcaca gaaaccgtac cacgtgccgt
     3421 gaccaactgc tctggcaact tagtttgatg cgcactaccc gagcccatcg aagaaaggcc
     3481 accaccaaac aatactgccg gcggcgcttc ccaaatcata atatccaata catcaccaat
     3541 atttagcgta ccagccgaag cataaccatc gccaaactga gtgaatgact gatttatctg
     3601 agccttatat aataactgag caaccgtatg attcacatca atcagctcca cttcaggaat
     3661 ttgaacttca gattgttgcc ctaaagagac aatttttttt gcgctggggc ctgatgaagg
     3721 aatcgcagag catcctacaa ttaaacttcc acacaataat aatactgcgt gacgaatata
     3781 aaatttcact ttaaacacaa gccaaatcct aatataatta taaatggcct aattatagca
     3841 cttaatcgaa ataaatttat gagtacgtag agtataatta gtattcttct ttccaacttc
     3901 cttatactta tatacttata gattctaaaa tcatgaaaag aattctttgc attacaggta
     3961 ccagagccga cttcggcaag ctaaaacctt tattagccta tattgaaaat cacccagacc
     4021 ttgaattgca tttgattgta actggtatgc atatgatgaa aacatatggc agaacctaca
     4081 aggaagtaac tcgagaaaac tatcaacata catatctgtt ttcaaatcaa atccaaggtg
     4141 aaccaatggg tgccgtttta ggcaatacca ttacgtttat ctctcgtcta tctgatgaaa
     4201 ttgaacctga tatggtcatg attcacggcg accgtttaga agcactagca ggcgcagctg
     4261 taggtgcatt aagcagccgt ttagtttgcc atatcgaagg tggtgaacta tctggtacag
     4321 tagatgactc cattcgtcat tctattagta aactttctca tatccacttg gtagcaaatg
     4381 aacaagctgt cactcgccta gtgcaaatgg gagaaaaaag aaagcatatt cacatcatcg
     4441 gctcccccga tttagatgtt atggcctctt ccaccctccc atccttagaa gaagtcaaag
     4501 aatattacgg tttaccatac gaaaattatg gtatttctat gtttcacccc gtgactacag
     4561 aagcacattt aatgccacaa tatgcggccc aatatttcaa agcattagaa ttaagtggcc
     4621 aaaatatcat tagcatctac cctaataatg acactggcac tgaaagtatt ctgcaagaat
     4681 tattaaaata tcaatctgat aaatttattg catttccttc cattcgattt gaatattttt
     4741 tagtcttatt gaaacatgct aaatttatgg tcggcaactc aagtgcaggt attagagaag
     4801 ctcctctcta cggtgtccct tcaattgatg ttggtacacg ccaaagtaac cgccatatgg
     4861 gaaaatctat tattcataca gattatgaaa ctaaaaatat ctttgatgcg attcaacaag
     4921 catgcagttt aggcaaattt gaagcagatg atacctttaa tggcggagat actcgcacca
     4981 gcacagaaag atttgctgaa gtaatcaaca atcctgaaac gtggaatgtt tctgctcaaa
     5041 aacgttttat cgatttgaat ctttaaatta tggaaaaaca aaatattgcg gttatacttg
     5101 cgcgccaaaa ctccaaagga ttgccattaa aaaatctccg gaaaatgaat ggcatatcat
     5161 tacttggtca tacaattaat gctgctatat catcaaagtg ttttgaccgc ataattgttt
     5221 cgactgatgg cgggttaatt gcagaagaag ctaaaaattt cggtgtcgaa gtcgtcctac
     5281 gccctgcaga gctggcctcc gatacagcca gctctatttc aggtgtaata catgctttag
     5341 aaacaattgg cagtaattcc ggcacagtaa ccctattaca accaaccagt ccattacgca
     5401 caggggctca tattcgtgaa gctttttctc tatttgatga gaaaataaaa ggatccgttg
     5461 tctctgcatg cccaatggag catcatccac taaaaaccct gcttcaaatc aataatggcg
     5521 aatatgcccc catgcgccat ctaagcgatt tggagcagcc tcgccaacaa ttacctcagg
     5581 catttaggcc taatggtgca atttacatta atgatactgc ttcactaatt gcaaataatt
     5641 gtttttttat cgccccaacc aaactttata ttatgtctca tcaagactct atcgatattg
     5701 atactgagct tgatttacaa caggcagaaa acattcttaa tcacaaggaa agctaaatgc
     5761 aaaacaacaa cgaatttaaa attggtaatc gttcagtagg ttacaaccac gaaccattga
     5821 ttatctgtga aatcggcatc aatcatgaag gctctttaaa aacagctttt gaaatggttg
     5881 atgctgccta taatgcaggc gctgaagttg ttaaacatca aacacacatc gttgaagacg
     5941 aaatgtctga tgaggccaaa caagtcattc caggcaatgc agatgtctct atttatgaaa
     6001 ttatggaacg ttgcgccctg aatgaagaag atgagattaa attaaaagaa tacgtagaga
     6061 gtaagggtat gatttttatc agtactcctt tctctcgtgc agctgcttta cgattacaac
     6121 gtatggatat tccagcatat aaaatcggct ctggcgaatg taataactac ccattaatta
     6181 aactggtggc ctcttttggt aagcctatta ttctctctac cggcatgaat tctattgaaa
     6241 gcatcaaaaa gtcggtagaa attattcgag aagcaggggt accttatgct ttgcttcact
     6301 gtaccaacat ctacccaacc ccttacgaag atgttcgatt gggtggtatg aacgatttat
     6361 ctgaagcctt tccagacgca atcattggcc tgtctgacca taccttagat aactatgctt
     6421 gcttaggagc agtagcttta ggcggttcga ttttagagcg tcactttact gaccgcatgg
     6481 atcgcccagg tccggatatt gtatgctcta tgaatccgga tacttttaaa gagctcaagc
     6541 aaggcgctca tgctttaaaa ttggcacgcg gcggcaaaaa agacacgatt atcgcgggag
     6601 aaaagccaac taaagatttc gcctttgcat ctgtcgtagc agataaagac attaaaaaag
     6661 gagaactgtt gtccggagat aacctatggg ttaaacgccc aggcaatgga gacttcagcg
     6721 tcaacgaata tgaaacatta tttggtaagg tcgctgcttg caatattcgc aaaggtgctc
     6781 aaatcaaaaa aactgatatt agggcgtgtc cctaatttga ataagcccaa aagagcccta
     6841 ttttaaccct atattccaac gaaatatagg caaacacaaa caatggcgag aaccgcaata
     6901 actgacaaca tatgggaaca attacaaaca accatgaaag cgcacggctg tcatcaatgg
     6961 aagaacgacc gtaccgtgat ggaagccata ctatggaagc taagaacagg tgcaccatgg
     7021 cgagacatac ctattgagct agggtcatgg aaaaccgctt ataaccgctt taaccgatgg
     7081 tctaaaaaag gcttgtggca gaattttttt ttgatctacg aaaagaaatt gacaaagaat
     7141 gggtattcat cgacggaagt tatgtacggt gtcatcaaca tgcaagtgga gctcggcgtg
     7201 gtttcgatag agcaattgga caaagccgtg gcggaaacac gacaaaaata cacctatgtg
     7261 tggactcgca tggaaatccg ctcgatttta aagtcactgg gggtaacgtg cacgacggtc
     7321 aagttgcaaa cgacttgata gaagtcatac aagaagccca gtattttatc gctgacaaag
     7381 ggtatgactc gcaagaaatc agagataaag cgatagaaca cggtatgaaa gctattatac
     7441 caaagcgtaa aaatgccaag aaacctaacc ctgattttga tagctacctt tataaattac
     7501 gccacttagt cgagaatgca tttgcacgat taaagcagtt tcgtagtatt gctactcgct
     7561 atgagaaatt agctcgtaat tttaaatcga tgctttactt ggcttgctct attattcatg
     7621 ctaagttaaa ttgaggacac gccctagtta ataataaaat agattaagct attcttaaat
     7681 tcagaatatt gcttatctat attaaaaatt tctaattttt aaggttctga ttgaaatcag
     7741 aaccttattt caactattac tttttactca taatcgaatt atatacttta ggactttata
     7801 atatggctgt tattatattt gttaacggaa ttcgggctgt aaatggcctt gttaaatcat
     7861 ctatcaatac tgcaaacgct tttgctgaag aaggactgga tgttcattta attaattttg
     7921 ttggcaatat tactggagca gagcatttat cccccccatt ccacttacat cccaatgtca
     7981 aaacctccag catcatagat ttatttaatg acattccaga aaatgttagc tgccgaaata
     8041 ttccttttta ttctatccat caacaattct tcaaagccga atacagtgcc cactataagc
     8101 atgttttgat gaaaattgaa tctttattat ctgaagaaga tagcattatc ttcactcatc
     8161 ctcttcaact ggaaatgtat cgtttagcga ataataatat taagtcaaaa gccaagctaa
     8221 ttgtacaaat tcatggtaac tatatggaag aaatccataa ctatgaaatt ttggcacgaa
     8281 atatcgatta tgttgattat cttcaaacgg tatctgatga aatgctggaa gaaatgcatt
     8341 cccatttcaa aatcaaaaaa gacaaattag tttttattcc aaacatcact tatcccattt
     8401 cattagaaaa aaaagaagct gatttcttta ttaaggataa tgaagacatt gataatgctc
     8461 agaaatttaa acgtatctct attgttggca gtattcagcc aagaaaaaac caattggatg
     8521 ccattaaaat catcaataaa attaaaaatg aaaattacat tttacagata tatggcaaat
     8581 ctattaataa agattacttt gaattaatta aaaaatatat taaagacaat aagttacaaa
     8641 accgtatctt attcaaaggt gaatcttccg agcaggaaat ttatgagaat acagatatcc
     8701 taatcatgac atctcaaagc gaaggcttta gttatatatt tctagagggt atggtgtacg
     8761 atatccctat ccttgcctat aattttaaat atggagcgaa tgattttagc aattataatg
     8821 aaaacgcttc agtttttaaa actggtgata tttctggaat ggcaaaaaaa ataattgagc
     8881 tattaaataa cccagaaaaa tataaagaat tagttcaata taatcacaat cgcttcttaa
     8941 aagaatatgc aaaagatgtg gttatggcta aatatttcac tattcttccg cgcagcttta
     9001 ataacgtatc attatcgtct gctttcagcc gaaaagaatt ggacgaattc caaaatatta
     9061 ctttttctat tgaagattct aatgatttag ctcatatttg gaatttcgag ctaaccaatc
     9121 ctgcacaaaa tatgaatttt tttgctttag ttggcaagcg aaaatttcca atggatgctc
     9181 atatccaagg aacacagtgt acgattaaga tagctcataa aaagacaggg aatttattgt
     9241 cgcttttact aaaaaaacga aatcagttga atttatcaag gggatatacc ttaattgcag
     9301 aagataatag ctatgaaaaa tatattggag caatatctaa taaaggtaac tttgaaatta
     9361 ttgcaaataa aaagaactca ttagttacta taaacaaaag taccttagag ttgcatgaga
     9421 ttccccatga actacatcag aataaattac tgattgcttt acccaacatg caaacgcctc
     9481 taaaaattac tgatgataat ttaataccta tccaagcctc cataaaatta gaaaagattg
     9541 gaaatactta ttacccatgt ttcttgccat ctggcatatt taataatatc tgcttagatt
     9601 acggtgaaga atccaaaatt attaatttta gtaaatattc ttataaatat atctatgact
     9661 caattcgtca tattgagcaa catacagata tatcggatat tatcgtttgc aatgtttatt
     9721 cttgggaact tattcgtgcc tcagttattg agagccttat ggaatttacc ggaaaatggg
     9781 aaaaacactt tcagacttct cctaaaattg attatcgatt tgatcatgaa ggtaagcgtt
     9841 cgatggatga tgtcttttca gaagaaacat ttattatgga atttccgcgt aaaaatggta
     9901 tagataagaa aacagcagcc ttccaaaata taccaaacag tattgtaatg gagtatccgc
     9961 agaccaatgg ttacagtatg cgcagtcatt cactgaaaag taatgtagtt gcggcaaaac
    10021 attttcttga aaaattaaat aaaattaagg tagatattaa atttaaaaag catgaccttg
    10081 caaacatcaa aaaaatgaat cgaattattt atgagcattt aggcattaac ataaatatcg
    10141 aagcatttct aaaaccacga ttagaaaaat ttaagcgtga agaaaaatat tttcatgatt
    10201 tcttcaaaag aaataatttt aaagaggtaa tttttccaag cacttattgg aatccaggta
    10261 ttatttgtgc tgcacataaa caaggtatta aggtatctga tattcaatat gctgccatta
    10321 ctccttatca tcctgcgtat tttaaatcac caaaatcaca ttacgttgct gataaattgt
    10381 tcttatggtc tgaatattgg aatcatgagc ttttaccaaa tccaacacga gagattggtt
    10441 ctggtgccgc atattggtat gcattagatg atgtgagatt ttcagaaaaa ctgaattatg
    10501 actatatctt tctatctcaa agtaggattt cttcgcgctt gcttagtttt gcaattgagt
    10561 ttgcattaaa aaatcctcaa ctacagcttt tattttctaa gcatccagat gaaaatatag
    10621 atttaaagaa cagaattatt cctgataatc ttataatctc cacggaatct tctatacaag
    10681 gcatcaatga atctcgcgtt gctgtaggtg tttattcaac tagcttattt gaggcattag
    10741 catgcggcaa acaaactttt gttgttaaat atccgggata tgaaattatg tcaaatgaaa
    10801 tagattcagg gttattcttt gcagtagaaa cacctgaaga aatgcttgag aaaacaagcc
    10861 cgaattgggt ggctgtggca gatattgaaa accagttttt tggccaagaa aaataatttt
    10921 taagttaatc tttaaaagtg caatattttt cttattttaa atattgcact ttattctttt
    10981 aatcatagga actcacatgt attctgaaca gggaattaat aatactatta acatatctac
    11041 tacatcttta accaatgcca cgcaactaac tgtgatcggg aataataact ctgtatatat
    11101 aggtaataat tgtaaaatcg tcagctccaa tattagatta aaaggaaata atataacctt
    11161 atttatagct gatgatgtag aaatcatggg actagtatgt tcccttcatt cggactgctc
    11221 tttacaaatt caagccaaga ccactatggg caatggagaa attaccattg ctgaaaaagg
    11281 taagattagt attggcaaag attgtatgct ggcacatgga tatgaaatta gaaatactga
    11341 tatgcatcca atttattctt tagaaaatgg cgaacgaata aaccatggta aagacgttat
    11401 tatcggtaat cacgtgtggc ttggccgcaa tgttactata ttaaaaggtg tatgtattcc
    11461 aaataatgta gttgtaggtt ctcatactgt gctatataaa agttttaaag aacctaattg
    11521 tgtaatagca ggttcacctg caaaaatagt aaaagaaaat attgtatggg gaagaaaaat
    11581 gtatcattca actatgtatg atgaccctac tttaaatgaa ttttataaat aacttagggg
    11641 cttaggaagc aaaatcatca aaaaacctga taagctccta atat
//

