LOCUS       MK551181               16526 bp    DNA     linear   BCT 05-MAY-2019
DEFINITION  Plesiomonas shigelloides strain O12H35 putative O antigen gene
            cluster, complete sequence.
ACCESSION   MK551181
VERSION     MK551181.1
KEYWORDS    .
SOURCE      Plesiomonas shigelloides
  ORGANISM  Plesiomonas shigelloides
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacterales incertae sedis; Plesiomonas.
REFERENCE   1  (bases 1 to 16526)
  AUTHORS   Xi,D., Wang,X., Ning,K., Liu,Q., Jing,F., Guo,X. and Cao,B.
  TITLE     O-Antigen Gene Clusters of Plesiomonas shigelloides Serogroups and
            Its Application in Development of a Molecular Serotyping Scheme
  JOURNAL   Front Microbiol 10 (2019) In press
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16526)
  AUTHORS   Xi,D., Wang,X., Ning,K., Liu,Q., Jing,F., Guo,X. and Cao,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2019) TEDA, Nankai University, Hongda Street 23,
            Tianjin 300457, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: VelvetOptimiser v. v2.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16526
                     /organism="Plesiomonas shigelloides"
                     /mol_type="genomic DNA"
                     /strain="O12H35"
                     /db_xref="taxon:703"
     gene            1..1974
                     /gene="rep"
     CDS             1..1974
                     /gene="rep"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase Rep"
                     /protein_id="QCH03149.1"
                     /translation="MLAGAGSGKTRVITNKIAHLIRGCGYQARHIVAVTFTNKAAREM
                     KERVAQTLGRGETKGLLVSTFHSLGLEILKREYKALGLKAGFSLFDDQDQMALLKELT
                     EDSLKGDKDLLSQLVQKISGWKNDMILPEQAKVRAKGERDILFALCYEQYARQLRACN
                     VLDFDDLILLPTLLLRTQEEVRERWQNRIRYLLVDEYQDTNTSQYELVKLLVGARARF
                     TVVGDDDQSIYSWRGARPQNLTLLRQDFPALKVIKLEQNYRSSQRILKSANILIANNP
                     HEFEKQLFSELGPGEMLRVLQANNEEHEAERVVGEIIAHRFLNKTQYKDYAILYRGNH
                     QSRTLEKMLMQNRIPYKISGGTSFFARAEIKDIMAYLRLLINPDDDTAFLRVVNTPRR
                     EIGPVTLEKLGTWAHSRGNSLFAASFDLGLEQHLQGRGLEALRQFTRWITEIADRAQR
                     GDPVAALRDLVHGIHYESWLYETSASPKAAEMRMRNVSTLFGWVTEMLEGKDGEELMT
                     INQVVQRLTLRDMLERGEDDAEADQVQLMTLHASKGLEFPHVFLIGMEEGILPHQTSL
                     DEDNVEEERRLAYVGITRAQKTLTFTLCKERRQYGELLRPEPSRFLHELPQDDVEWEV
                     RKAPVSAEQRQQRGRQGVAGLREMLAQAKKRPE"
     misc_feature    1..16526
                     /note="putative O antigen gene cluster"
     gene            complement(2129..2512)
                     /gene="rfaH"
     CDS             complement(2129..2512)
                     /gene="rfaH"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="QCH03150.1"
                     /translation="MLTVKKQRRGQWQEEAEALFANYLFVHFNPDQLSVTRVQATRGV
                     ARLVRFGETLARVPDEVLIDLARRYNPLVALPEEGKVCSQPMCTALQQALADIEQESS
                     GEVRALRFLQLLQDHRLLASRHREE"
     gene            complement(2875..3300)
                     /gene="c5"
     CDS             complement(2875..3300)
                     /gene="c5"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="QCH03151.1"
                     /translation="MRAKSICAKPRQAALTLLAFLLLGVSCWVAAADMSDDAIIERIK
                     PVGGVYLADASATAKPSGPRTGEQVYNSACIACHGSGALGAPKFRDAAQWAPRLKQGI
                     DTLQKHAIAGLNQMPARGTCTDCSDDEIKAAIEYMISGL"
     gene            complement(4744..5943)
                     /gene="orf4"
     CDS             complement(4744..5943)
                     /gene="orf4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QCH03152.1"
                     /translation="MVFSYHSFGKEKNVWHLKEAPVTPLFGIDRYGYSGWAEITNLPR
                     KRQEISAISDKHAEEVIEKYRRQFKEKRISKYPQPDEQDVELPAEYIFFPLQVSNDPV
                     SQFSPFNMLDMLKRAAEAARRTGTTLLVKRHPFCPSVAVKRTLQQLTEDNPQVKVVNL
                     NVHTLIEHAKAVMTVNSGVGIEALIDGAAVYAAGKSEWFAAANSIASLEDIDAIFSEA
                     PRYMDSWQKKLIAFLLDSYWVSPSDYAAIERKIEQSIAQFDPDYGIDSALPYASEVFL
                     PIVLDLQGRLEYESRRAKLAIFDFDGLNGSIERLDAIRAQQDAQIAQLRHESEQRIAD
                     LEELLQQKQREIGQKESELEQKENEIARVKAELEKHQVQLISLANDLSNSRMESEHLH
                     SGLNHQR"
     gene            complement(6997..7884)
                     /gene="wbbN"
     CDS             complement(6997..7884)
                     /gene="wbbN"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="QCH03153.1"
                     /translation="MNVVALIVTFNRLEKLRTCLATTLGMEFRNVVVVDNASTDGTGD
                     WLAGIRDERLHCLTLVSNIGGAGGFKKGAEYITHHLDYDWVIFYDDDAYPDANMLSIL
                     PTLPETHQVFCCRVTDMQGNICRMNLPFRHLPHSLADHVRYALKPADFIPAYDMPCSV
                     ETLSFVGVIFSHSVLAEHWNAIRDELFLYYDDLYFGYYLTQLGVNIRYSPEITFVHDI
                     SLAGRDIRPQWKVYYLIRNLFLSRRCFVQRPPFSWPSIGFRVIKYFWLKGQSGKKAYI
                     SYLWRGIVDGLRQRTGMRH"
     gene            complement(7881..9035)
                     /gene="glf"
     CDS             complement(7881..9035)
                     /gene="glf"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranosemutase"
                     /protein_id="QCH03154.1"
                     /translation="MSNKSILIVGAGFSGAIIGRQLAEQGHKVRIIERRAHIAGNCFD
                     ERDSATNVMVHVYGPHIFHTDNQRVWEYVNRYGHMMPYVNRVKATVKGQVFSLPINLH
                     TINQFFKTTCSPDEARNLIEEKGDKSISDPQTFEEQALKFIGRELYEAFFKGYTIKQW
                     GMQPSELPASILKRLPVRFNYDDNYFNHKYQGMPRDGYTKIVEQILEHENITVELNTE
                     FERQDVGNYDHVFYSGPLDAYYGYCHGRLGYRTLDFEKFSYDGDYQGCAVMNYGSVDV
                     PYTRITEHKYFSPWEKHQGSVCYKEYSRTCEEDDIPYYPIRQVGEMALLEKYLSLAEQ
                     ESDVTFVGRLGTYRYLDMDVTIDEALKTADAYLASLTDDTKMPAFVVNTR"
     gene            complement(9059..10891)
                     /gene="wbbM"
     CDS             complement(9059..10891)
                     /gene="wbbM"
                     /codon_start=1
                     /transl_table=11
                     /product="WbbM"
                     /protein_id="QCH03155.1"
                     /translation="MHPLHVGKANSYNEIGCIGDNTGDNISFKNPFYCELTAHYWVWK
                     NAQSADFIGFMHYRRHLNFSDNQSFPEDNWGVVNYPVLNDTYEAMFGLDDQSIARCLQ
                     DTDIIVPKKWAVTAANSKNNYDHYDRGEALHIKDYQAALDVLNEMYPEYMPAAEKFNH
                     SVDGYYTNMYVMRRDIFEHYSEWLFSILNKLEERIAFNNYNAQEKRVIGHISERLFNI
                     YLIHQQESNHYRLKELQRTFIMQETFNGSLSPAFEKNNVPIVICFDDNYALSGGALLN
                     SIIANATPAHNYDVVVLENNVSPLSKQRLNMLVANRENIRLRFFDVNAFSEIKEVFTR
                     AHFSAATYARLFIPRLFRKYDKVVFIDADTVVESDVAELMHTDIGNNLVAAVKDIVME
                     GFVKFGAMSDSSDGVMPAAEYLQKSLGMANPDMYFQAGIIVFNIHQMNTEGTFEKLMF
                     AMKEKKYWFLDQDIMNKVFFDRVHYLPLAWNVYHGNGNTDDFFPNLKFSTYMAFLEAR
                     CQPKMIHYAGENKPWNTTRVDFFENFMKYISNTPWQRDVYLRLNPVTPADVPTTEVHH
                     APVLLQTKIKRRLMPYLNRVAPQGTARRNQLTKYYYKVRRVILD"
     gene            complement(11056..11700)
                     /gene="wzt"
     CDS             complement(11056..11700)
                     /gene="wzt"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="QCH03156.1"
                     /translation="MNLLRGRKYLAIENITFSVNKGEAVALIGRNGAGKSTSLGLVAG
                     VLKPSKGSVSVEGRVASMLELGGGFHPELTGRENIKLNATLLGLRRKEVNARLDKIIE
                     FSELGEFIDEPIRVYSSGMLAKLGFSVISQVDPDILIIDEVLAVGDISFQKKCINTIN
                     DFKAKGVTILFVSHNMADIARICDRVIWIENHKLKATGDAETVIEQYKAAMLSS"
     gene            complement(11802..12704)
                     /gene="wzm"
     CDS             complement(11802..12704)
                     /gene="wzm"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="QCH03157.1"
                     /translation="MSKTISMACFFVTHKIFKCLDSVQQDTLDIRLLYIRILYGILSN
                     NMKLNLGYLFDLVFVITEKDLKVRYKSSFLGYLWSIANPLLFAMIYYFIFKLVMRVQI
                     PNYTAFLITGLFPWQWFASSATNSLFSFIANAQIIKKTVFPRSVIPLSNVMMEGLHFV
                     CTIPVIIVFLYIYGMSPSWSWLWGIPLIAGGQIIITFGLALLFSTLNLFFRDLERFIS
                     LGILLMFYCTPILYGADMIPANFSWMVEYNPLASMILSWRDLFMNGNVEYERIAFLYL
                     SGIVVLLIGMWTFNKLKYRFAEIL"
     gene            complement(12749..13936)
                     /gene="wbbO"
     CDS             complement(12749..13936)
                     /gene="wbbO"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="QCH03158.1"
                     /translation="MKSRLSVSLVLRWEMKVLFFVNAAWYFDLHWLDRAKSLIDEGHE
                     VHVVTSVTDEAIRESIESHGITCWSIEIPRFSKNPILNIKILLAFRQKLKEIKPDLVH
                     LITIKPILLGGALLRFTQIPFVVSFVGLGRMFNNTHRVTDTFLKRTITTFYSAILSKN
                     KNTRAIFEHIDDYKTLSQYVDFLDDQVHIIDGAGIDIEKYKYQPEYKDKELNILFASR
                     MLWSKGLGVLVDAVELVKQRGIAVNLKVAGIIDSNDPDKIDASVISSWNEERKIQWLG
                     TRKDVENLIRDSNLVILPTRYAEGVPRIILEACSMGRACIVSNVPGCKSVIQDGVNGL
                     VLKTGTATELADYIEKLAVNETLRRSYGLISSNIIKQRFAKDIVIERTIFVYESLLRS
                     HTV"
     gene            15825..16526
                     /gene="aqpZ"
     CDS             15825..16526
                     /gene="aqpZ"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="QCH03159.1"
                     /translation="MFKPFSAEFFGTFWLVLGGCGSALISAAFPQLGIGFLGVALAFG
                     LTVVTMAYAVGHISGAHFNPAVTLGLWAGGRFPAARVLPYIIAQVIGGIAAAAVLYGI
                     ASGKAGFDATTSGFAANGYGIHSPGGYALSACMLSEFVLSAFFVIVIHGATEKRAFAG
                     FAPLAIGLTLTIIHLVSIPVTNTSVNPARSIAAAVFQGTWALDQLWMFCLIPSLGGIA
                     GGLIYRALLARPAEA"
BASE COUNT     4451 a   4039 c   3725 g   4311 t
ORIGIN
        1 gtgctggctg gtgccggctc gggaaaaacc cgcgtaatta ccaacaaaat tgcccatctg
       61 atccgcggct gcggttatca ggcgcgccac attgtggcgg tgacctttac caacaaagcc
      121 gcgcgcgaga tgaaagagcg ggtagcgcag acgctcggtc gtggtgaaac caaaggcttg
      181 ttggtgtcca ctttccactc tctgggcttg gaaatcttaa agcgcgaata caaggcgctg
      241 gggctgaaag ccggtttttc gctgtttgac gatcaagacc aaatggcgtt gctcaaagag
      301 ctgaccgaag acagcctcaa aggtgataaa gacctcctca gccagctggt gcaaaaaatc
      361 tccggctgga aaaatgacat gatcttgccc gagcaggcca aagtgcgcgc caaaggtgag
      421 cgcgatatct tgtttgccct gtgttatgag cagtacgcac gccagctacg cgcctgcaat
      481 gtgctggatt ttgacgatct gattttgctg ccaaccttat tattgcgcac ccaagaagag
      541 gtacgagagc gttggcaaaa tcgcattcgc tatctattgg tggatgagta tcaagatacc
      601 aacaccagcc agtacgagct ggtgaaattg ctggtcggcg cgcgtgcccg tttcaccgtg
      661 gtaggggatg atgatcagtc gatttattca tggcgcggtg cgcgtccgca gaatttaacc
      721 ttgctgcgcc aagattttcc ggcgttgaaa gtgatcaagc tggagcagaa ctaccgctcc
      781 tcgcagcgca tattaaaatc ggccaacatc ctgattgcca acaacccgca tgagtttgaa
      841 aaacagctgt tctccgagct cggccccggt gagatgctgc gtgtgctgca ggcaaacaac
      901 gaagagcatg aagctgagcg cgtggtgggt gagatcatcg cgcaccgttt cttgaacaag
      961 acccaatata aagattacgc cattttgtac cgcggtaatc accagtcgcg cacgctggaa
     1021 aagatgctga tgcaaaaccg cattccgtac aagatctcgg gtggtacctc tttctttgct
     1081 cgcgccgaaa tcaaagacat catggcctac ttgcgcctgc tgattaaccc cgatgacgac
     1141 accgcgttcc tgcgcgtagt gaatacccca cggcgtgaaa tcggtccggt gaccttggaa
     1201 aagctcggta cgtgggcgca cagtcgtggc aatagcctgt ttgccgctag ctttgatttg
     1261 ggattggagc agcatttgca ggggcgtggg ttggaggctc tgcggcagtt tacgcgttgg
     1321 atcaccgaga ttgccgatcg cgcccagcgt ggcgatccgg tcgccgcgct gcgtgatttg
     1381 gtgcatggca tccattatga aagctggctc tacgagacct ccgccagccc aaaagcggcc
     1441 gagatgcgca tgcgcaacgt ctctaccctg tttggttggg taaccgagat gctggaaggg
     1501 aaagatggcg aggagctgat gaccatcaat caggtggtgc aacgcctaac cttgcgcgac
     1561 atgttggagc gcggcgaaga tgacgccgaa gccgatcaag tgcagttaat gacgctgcac
     1621 gcctcgaaag gactggagtt tccgcatgtc ttcttaattg ggatggaaga ggggatcttg
     1681 ccgcaccaga ccagtttgga tgaagacaat gtggaagaag aacgccgttt ggcgtatgtc
     1741 gggatcaccc gcgcccagaa gaccttgacc tttacgttat gtaaagagcg ccgtcagtac
     1801 ggtgagttgc tgcgcccaga gccgagtcgc ttcttgcacg aactgccaca agatgacgta
     1861 gagtgggaag tacgcaaagc gccagtcagt gccgaacagc gccagcagcg tggccgccaa
     1921 ggggtggccg gattacgtga gatgttggcg caggcgaaaa aacggccaga gtgatcttac
     1981 tggccatggg cttagtgttt acgcatataa gtgtctactc atataagcga ctacagagat
     2041 aagtgcctaa atatttaggc gcttataaat gcggaagacg cccgcaacgg gcgtgcgcac
     2101 tatgacattg gaatgccgga acgcaggatt attcctctcg gtgacgggaa gccaacagac
     2161 gatgatcttg cagtaattgc aaaaagcgta gcgcacgaac ttccccactg ctttcttgct
     2221 cgatatccgc caaggcctgt tgtaaggcgg tacacatcgg ctgactacag actttaccct
     2281 cttccggtag tgcaaccagc gggttgtaac gtcgcgctaa atcgatcagc acctcatccg
     2341 gcactctcgc caatgtttcg ccaaagcgca ccaaccgtgc cacgccgcgc gtagcttgca
     2401 ctcgcgtgac actcagctga tcgggattaa agtgcacaaa caggtaattg gcaaacagcg
     2461 cttcggcctc ttcctgccat tgacctcgac gctgtttttt taccgtcagc atcggacaaa
     2521 aatagcggat atcttgcaac tccagatgtt gcgccgcccg actttgctgt ccgcgtttac
     2581 aataaagaag ataccaggcg tccatagata gttttgtgat gaaaataatg actgcagtat
     2641 gccacagagt ggatagccgg tcagtctttg ggtgtgatgt aacagtcagt ttaattaacg
     2701 cgaaggccgc acaaagcacc gttcgcgcaa aataccgccg gtgaattgtc agttagagcc
     2761 agttaagtca gagcaatcgg gcgaaaagaa taaaataatg ggcaacagat atagaaggag
     2821 gcacttaggc ctcattctat ttgcgcgata ttcttcttta gtatgctgag tagcttataa
     2881 cccgctaatc atgtattcga tcgcagcttt gatttcatca tcactacaat cggtacaggt
     2941 tccgcgtgcc ggcatctgat ttaaaccggc gatagcatgt ttttgcaggg tatcgatacc
     3001 ttgcttcaga cgtggcgccc actgcgcagc atcgcgaaac ttaggtgcgc ctaaggcacc
     3061 gctgccatga caggcgatac aggcgctgtt atacacctgc tcaccggtac gcggcccact
     3121 gggtttagcg gtggccgaag catcagccag atacaccccg ccgaccggtt tgatgcgttc
     3181 gataatcgca tcatccgaca tatcggctgc ggcaacccag caggatacgc ccagcagcag
     3241 gaaggccagc agagttaacg ccgcctggcg aggcttagcg cagatagact tggcacgcac
     3301 gaagacagtg ttcacagatt ttctccctaa ataaccagcg gcatccttta tcctgcgttc
     3361 attcttagcg cctgccccat taacgcgatt gcacttgcca ttatcacctt gggcaagtgc
     3421 tcgaaatcct cacgtacttg gatgtacgct ccggtttctg cgcgctggcc aagatgaaac
     3481 tggccgcgtc aatcatcctt actggggtag gctttcatcg ggctgagcag ggctgtgaac
     3541 cgcagcggtt gaatattgac cactcggttg tgttgccaga ttataagcta atcgtaaata
     3601 aattgaagca attgcgcggg gttctttttt gcaaggcagt cagcgtgcga cagcttgtac
     3661 agcccagcct cacagaattt acaaacatac tgttgtcttt ttgacggcag atttggcttt
     3721 acacaggcca caacaaaata caacatcaaa cggatggact gattcttagg caatcactat
     3781 ctacataaag agcgattcga agtgtacccc tagcacgcaa gtttcagtcg cgcttcaagg
     3841 ccgatccgtc actctatacg agaaagcata ccctctataa gagtaatgct taaagtctgc
     3901 tcatggccag tttttgttgg atcttgcgct cattttacct aataacacta caccatttat
     3961 tgtcagcgat gctggcttca aagtgccatg gtatacatca gttgaatacg gttggtactg
     4021 gctaagtcga gtcagaggga acgcgaaatt tgcagatcta ggtgccgata gttttcacta
     4081 tgtcagtgag ataaatgcca ttgcctcaag taaagcaaaa atgctaggag gttataaaaa
     4141 gctaaccaag agtagctcga tttcatgcca gttcacgctt tataaatctc gttcggtggg
     4201 cagaaagaat cagcgttcta cacgaacaga ttgtcatcat tactcaccaa aggtttactc
     4261 tgcctcagta aaggagtcat gacgggtact tgctactaac ttaccggcag gacaggtatg
     4321 agaacaccaa agcaactcgt gcgaatctac gctaagcgca tgcaaataga aaaaacgtaa
     4381 agggattcgg gttacggcat agccgaacca gtaatcccaa acgctttgac atcatgttgt
     4441 tgattgccct catgctccaa ctcacttttt ggctcgcagg ggggtcatgc caagcagcaa
     4501 ggttaggata aacacttcca agccaatacc atcagagaac agaatgtact ctcaatagtt
     4561 cgtttaggca tggaagtatt gaggcgtttg gactataaga taacaaggga agaactgcta
     4621 gctgctgcaa aaatagttgc ttaaaaccta atcaaacatg gctatgcgca gggagaatta
     4681 tgaggatagc tttcagctct ttgcaggctt ttccgcaacc cttcaatctg ctctgtcacc
     4741 tgctcatctt tgatgattca gcccagaatg caagtgctcg ctctccatgc gggagttgga
     4801 aagatcgttc gcgagtgaaa tcagctgcac ctgatgtttt tccagctcag ctttcacgcg
     4861 tgcgatttca ttttcctttt gttcaagctc actttccttc tgcccgatct cgcgctgctt
     4921 ttgctgaagc agttcctcaa gatcagcaat acgctgttca ctttcatggc gaagttgtgc
     4981 gatttgcgca tcctgttgcg cacgtattgc gtccagtctt tcgatgcttc cattaagtcc
     5041 gtcgaaatca aatatggcta atttagcgcg gcgggactca tattcaaggc gcccctggag
     5101 atcgagcacg ataggcagga aaacttccga ggcataaggt agtgcggaat caatgccata
     5161 gtcaggatca aactgcgcga tactctgttc gattttacgc tctatggcgg catagtctga
     5221 aggcgaaacc caataactgt ctaataaaaa agcgatcagt tttttttgcc agctatccat
     5281 atagcggggc gcttcgctga aaatagcatc gatatcctcc agtgaagcga tcgaattggc
     5341 ggccgcaaac cactcgcttt tccccgccgc ataaaccgct gcgccatcaa tcaacgcttc
     5401 gatacctacc ccagagttta ccgtcatcac ggccttggca tgctcaatca gcgtatgcac
     5461 attaaggtta acaaccttaa cctgcgggtt atcttccgtc agttgttgaa gcgttctttt
     5521 cacggcaacg gaaggacaaa atggatggcg ctttaccagc agcgtagtgc cggttcgccg
     5581 ggccgcttcc gccgctcttt taagcatgtc cagcatattg aaagggctga actggctaac
     5641 aggatcgtta gaaacctgga gagggaaaaa gatatattcc gcgggcagtt cgacatcttg
     5701 ttcatccggc tgcggatatt tagatatccg tttctcttta aattgtcggc gatatttttc
     5761 aatcacctct tcagcatgct tatcactgat agcggaaatt tcttgccgct tgcgcggtag
     5821 gttagtaatt tccgcccaac cggaataacc ataccggtca atgccaaaaa ggggggtcac
     5881 cggcgcttct ttcagatgcc agacattttt ctctttgcca aaagagtggt aggagaaaac
     5941 caatgctccc ttctctggct ttctggctgc tcgatcggcc ccaaaaggca cctttatcag
     6001 atgtaacgga acattaaatt tgctaagttg aatcaggaaa ttggcgaaaa aagtccgatt
     6061 cttctctccg tcaaccggat tatcgaacca ggatacgggt acttctacca ctatcttttt
     6121 cagcatacct atctcatact gttttgttaa aacaccatta ctttacagaa tagttgacgg
     6181 gattgcatca gccgccgcgg cttcctggtg tatatcaaaa tatcggtaat gtatctaact
     6241 tagtgtcaca tgtattttcc gataaaagca ccaatgactt ttccatgaag ttcaacatag
     6301 cgggaaaagc cgatgcttct gcgagccaga tgcttgattc gagctctcaa tgttagattt
     6361 ttgcgctcaa ttcgttgagt aaagatctta tcggtcagga gcttttcctt aggaagctct
     6421 cttgcataga ttcccctaac ataccgatgt tagagggaat gagcagtgcc agtaattcac
     6481 ggcaagttgc atcggctcgt ggtggaccaa aagtgtaggc cagtatctcc cgtttttgtg
     6541 tagtacgcat accaaagcct gtgctgtcga gccttactac cgataaaact ccattgttcg
     6601 tcaagttcac agataagcct cacatcagca tgagccaccg aagatgatgt tattcgccgt
     6661 ggcgagaatt ttttcaagtc cgtatgatgg tattgcgaac tcctgcaccg ttaaaagcca
     6721 ttttcttgat ctattcttta acactcggct tgctggcctc gtatgagtaa gcaatttgaa
     6781 acactcgcta gcagtcttgt cagcggaacc gatcatatcc tttcgggttc tgtccatgac
     6841 gataaaccag agcagcctga caacggcgac aatgaacgat aatgctggct atgggtaaac
     6901 ccaaaagcca gcattatacc tcgaattcaa ccaattagag acatcatcaa aatatcaaca
     6961 ttacaggtat gtttctataa cgacacgcct cactggctaa tgccgcatac cggtcctttg
     7021 gcgcaaccca tccacgatgc cacgccacaa ataagaaata tatgcctttt tgccgctctg
     7081 gcccttcagc cagaaatatt taattacccg aaaccctatc gacggccagc tgaacggagg
     7141 gcgttgcaca aaacagcgcc gggaaagaaa cagatttctg atcagataat acactttcca
     7201 ttgcggcctg atgtcacgcc cggccaaaga aatatcatgt acaaatgtta tctcaggaga
     7261 ataacggata ttgacgccta attgtgtcag ataataacca aaataaagat cgtcgtaata
     7321 caggaataat tcgtcgcgga tagcgttcca gtgctcagct aatacgctgt gggaaaaaat
     7381 cacgccgacg aatgacaagg tttccaccga acaaggcatg tcatatgcag ggataaagtc
     7441 agcgggtttt aatgcgtaac gaacatgatc cgccagcgaa tggggaaggt gccggaaagg
     7501 caggttcata cggcagatat ttccctgcat atccgtgact cggcagcaga atacctgatg
     7561 tgtttcaggg agggttggca gaatacttag catattagca tccggatatg catcatcatc
     7621 gtagaagata acccagtcgt aatctaggtg atgcgtgatg tactccgccc cttttttaaa
     7681 tcctcctgcg ccgccgatat tgctgaccag tgtaagacaa tgcaaacgct catccctgat
     7741 cccagccagc caatcacccg ttccgtcagt ggaggcgtta tccaccacca ccacgttccg
     7801 gaactccatg cccagcgttg tcgccaggca ggtacgaagc ttctcaagac ggttaaacgt
     7861 gacaatcagt gcaacgacat tcatcgagta ttgaccacaa atgcaggcat tttcgtatcg
     7921 tcagtcaaag atgccagata agcatccgct gttttcaatg cttcatcaat cgttacgtcc
     7981 atatctagat agcgataggt gccgagccgt ccgacaaacg ttacgtcaga ctcctgctca
     8041 gccagagaaa ggtatttctc cagcaatgcc atctcaccga cctggcgaat cgggtaataa
     8101 ggaatgtcat cctcttcgca ggtacggctg tattctttat agcacacgga gccttgatgt
     8161 ttttcccatg gagagaaata cttatgctcc gttatccggg tatagggcac atctacggaa
     8221 ccgtaattca tgacggcaca tccctgatag tcgccgtcgt agctgaactt ctcaaaatcc
     8281 agcgtacggt accctaatcg cccatggcag tagccgtaat aggcatcaag ggggccgctg
     8341 taaaatacgt gatcataatt gccaacatcc tgacgctcga actccgtgtt caattcaacg
     8401 gtaatgtttt catgctcaag aatctgttca actattttgg tataaccgtc tctaggcatt
     8461 ccttgatatt tatggttgaa gtagttatcg tcgtaattaa aacgtaccgg caagcgcttt
     8521 aatatcgaag ctggtaattc actgggctgc attccccact gcttgatggt gtagccctta
     8581 aagaaagctt catacaattc tcgtccgata aacttcaggg cctgttcttc aaaagtctgg
     8641 ggatcggaga tgcttttatc gcctttctct tcaatcaggt ttcgagcttc atccggggag
     8701 caggtggttt tgaaaaactg gttaatcgta tgaaggttga tcggtaaaga aaatacctgt
     8761 cccttgaccg tcgctttgac acgattaacg taaggcatca tgtgcccgta acgattcaca
     8821 tattcccata cccgctgatt gtcagtatga aaaatatgcg ggccataaac atgcaccatc
     8881 acattggtcg cactgtcacg ctcatcaaag cagttccccg caatatgcgc tcggcgctca
     8941 ataatgcgga ccttgtggcc ttgttcagcc aactgtctgc cgataatagc gccggaaaac
     9001 ccagcaccaa cgatcaaaat agatttatta ctcataaaag atgctcttat cgcttacgtt
     9061 aatctaaaat cacacgacgg actttgtaat agtattttgt taactggtta cgccgtgcag
     9121 taccctgcgg cgcgacgcgg ttcagatatg gcataagacg tctcttgatt tttgtttgta
     9181 acagcaccgg ggcatgatgc acctccgtgg taggcacgtc tgccggggtt actggattaa
     9241 ggcgcagata gacatccctt tgccacggcg tattgctgat gtacttcata aagttttcga
     9301 agaaatccac cctggttgtg ttccatggtt tattttctcc ggcatagtga atcatcttgg
     9361 gctgacaacg tgcctcaaga aatgccatat aggtcgagaa cttcaggttc gggaagaagt
     9421 catctgtgtt accattacca tgatagacgt tccacgccaa cggaagataa tggacgcgat
     9481 cgaagaatac cttgttcata atatcttgat cgaggaacca gtatttcttt tccttcatcg
     9541 caaacatcag cttttcaaac gtgccctctg tgttcatctg atgaatgtta aagacgataa
     9601 tcccagcctg gaaatacata tccggattag ccatacccag tgatttttgc aggtattctg
     9661 ccgccggcat tacgccatcg gaggaatctg acatcgcgcc aaatttgacg aagccttcca
     9721 ttacgatatc tttgaccgcg gcaaccaggt tattgccgat atcagtatgc atcaactcag
     9781 cgacatcgct ttccactacg gtatccgcat caataaatac cactttgtca tatttgcgga
     9841 ataagcgggg aataaacaga cgggcatagg tcgccgcgct aaagtgagcg cgggtgaaca
     9901 cttctttgat ttcagagaac gcattcacat cgaagaaacg cagtctgata ttttccctgt
     9961 tagcaaccaa catgttcaaa cgctgcttgc tcaacggcga aacattattc tccagcacca
    10021 cgacatcgta gttatgcgcc ggagtagcgt tggcaatgat ggagttcagc agggcgccgc
    10081 cactcaaggc atagttgtca tcaaaacaga tgacgattgg cacgttattc ttttcaaagg
    10141 caggagacag agaaccgttg aacgtttcct gcataatgaa tgtgcgttgc aactccttga
    10201 gacggtaatg attgctttcc tgctgatgaa tcagataaat attgaaaagt cgctcagaaa
    10261 tatgtccgat gacgcgtttc tcttgagcat tatagttatt gaaagcgata cgctcttcca
    10321 gcttattcag aatagagaaa agccactccg agtaatgttc aaaaatatca cgacgcatta
    10381 catacatatt ggtgtagtaa ccgtctacgg aatgattaaa cttttctgcc gccggcatat
    10441 actcaggata catttcattc agtacatcga gcgctgcctg gtaatcttta atatgcagcg
    10501 cctcgccgcg atcataatgg tcataattat tcttactgtt ggctgccgtt accgcccatt
    10561 tcttgggtac gataatatcc gtatcctgca aacagcgggc aatgctctga tcgtccagcc
    10621 caaacatcgc ttcataggta tcgttcaata caggataatt gacgacgccc cagttatctt
    10681 ctgggaaaga ttgattatct gagaagttca gatgacgacg ataatgcata aaaccgataa
    10741 agtctgccga ttgtgcattt ttccataccc aatagtgcgc cgtcagctca caatagaacg
    10801 ggtttttaaa ggaaatattg tcgccggtat tatcaccgat gcagccaatt tcattataac
    10861 tgttggcctt gccaacgtgc agaggatgca ccgagtcagc gttgagaaaa gcgctgggct
    10921 tatggtgaca agtatagatt ttaatacttt tcattaatct attccaaaaa caataatgac
    10981 aggctacata gccattgatt aattacaatc agctagtcaa ttgaataata gctcattccc
    11041 ttgcaaacct tatgcttatg acgaaagcat cgcggctttg tattgttcga tgactgtttc
    11101 agcatcacct gttgctttca atttgtgatt ctctatccaa atcaccctgt cgcatattct
    11161 agcaatatca gccatattat ggctgacgaa caaaatagta acccctttgg ccttaaaatc
    11221 attgattgta ttgatacatt tcttttggaa agaaatatct cctaccgcca gaacctcatc
    11281 aataatcaag atatcaggat caacctggct gatgacggaa aaacctaatt tcgccaacat
    11341 accgcttgaa taaacccgga taggttcatc aataaactcg cccagctcag agaattcgat
    11401 aatcttatct aggcgggcgt taacctcttt acgtcttaag cccagtaacg tcgcattgag
    11461 cttaatattt tcacgccccg tcagctcagg gtgaaaccct ccccccaact ccagcataga
    11521 ggctacacga ccttcaacgc tgacgcttcc cttacttggc ttcaaaaccc ctgcaaccaa
    11581 gcccagcgag gtgctcttac cggcgccatt acggccgatt aaagctacag cctcaccttt
    11641 attaacgctg aaggtaatat tctctatcgc aagatactta cgcccacgca acagattcaa
    11701 cgcacgccgg ggatgaaata tcaggtcttt aacacccgac ccaatatgat gatacagcgg
    11761 atattccttc gtcacaccgc gaaattcaat aactgcgctc attacaaaat ctcagcaaaa
    11821 cgatatttaa gtttattgaa tgtccacatg cctatcagta gaacaacaat acccgataaa
    11881 taaaggaagg ctatgcgttc atattctacg tttccattca tgaacaaatc cctccagctc
    11941 aaaatcatac tggccaaagg attatactcc accatccaag agaaattagc cggtatcata
    12001 tccgcgccat aaagaatcgg ggtgcagtaa aacatcagta gaattcccag actaatgaac
    12061 ctttcgagat ccctgaaaaa cagattcaat gtggaaaaca gcagtgccag accaaaagtg
    12121 ataataatct ggcctccagc gataagcggg attccccaca gccatgacca tgaaggagac
    12181 ataccataaa tgtataggaa aacgataatg acggggatag tacagacgaa atgcaatccc
    12241 tccatcatta cgttactcag aggaattacc gagcggggaa aaacagtttt cttaattatc
    12301 tgtgcattag caataaagga aaacagcgaa ttagttgctg agcttgcaaa ccactgccat
    12361 ggaaacaaac cggtaatcag gaatgccgtg taattaggga tttgcacccg catgaccaac
    12421 ttaaagataa aatagtagat cattgcaaaa agcagcgggt tggcaataga ccaaagatag
    12481 ccaagaaaac tacttttata gcgaactttt aaatcctttt ccgttataac aaacactaag
    12541 tcaaataaat agcctaaatt taacttcata ttattactca atatgccata taaaattctg
    12601 atgtataata gtcttatatc aagcgtatct tgctggacac tatccagaca tttgaaaatt
    12661 ttatgagtaa caaaaaagca tgccatggat atggttttac tcaaaaataa ccaacgctaa
    12721 ctattataat gttacaagcg aacagatctc aaacggtatg actccgtaac aaagactcat
    12781 acacaaaaat tgttctctca ataacaatat cttttgcgaa ccgttgctta ataatattag
    12841 aagaaatgag accatagctc cttcgcagtg tttcgttaac cgctaacttt tctatatagt
    12901 cagctaactc agttgccgtg cctgttttca acacaaggcc attgacccca tcctggatca
    12961 ctgatttgca tcccgggacg ttactgacaa tacaggccct tcccatagaa catgcttcca
    13021 aaatgatgcg tgggacacct tccgcatatc ttgtcggtaa aatgactaag tttgaatccc
    13081 tgataagatt ttctacgtct ttgcgggttc ccagccactg aatcttcctt tcttcattcc
    13141 aggagctaat cacggaggcg tctattttat cgggatcgtt actgtcgata atacctgcaa
    13201 cctttagatt aacggcaata ccgcgctgct taaccagctc aacggcgtca acaagcacgc
    13261 ccagcccctt cgaccacagc atccggctgg cgaaaaggat gttgagctct ttatccttat
    13321 actccggttg gtatttatat ttctcaatat caataccggc gccgtcgatg atatgaacct
    13381 gatcgtcaag aaaatcaaca tattgactca gtgtcttgta gtcatcaatg tgctcaaaaa
    13441 ttgcacgggt attcttattt tttgaaagaa tcgcactgta gaacgtcgta attgtacgtt
    13501 tcaaaaatgt atcagtaacc cggtgcgtat tattgaacat acggccaaga ccgacaaagc
    13561 tcaccacgaa gggaatttgg gtaaagcgga gtagcgcccc gccaagaaga atgggcttaa
    13621 tggttatcag gtgaacgaga tcaggcttta tctctttaag tttctgccgg aaagccaaca
    13681 gtatttttat attgagtatc gggttcttag aaaaacgggg aatctcaata ctccagcacg
    13741 taatgccatg actctcaata ctttctctta tcgcctcatc ggtaaccgat gttactacat
    13801 gaacttcatg tccctcatct ataagagact tggcacgatc aagccaatgc agatcaaagt
    13861 accaggctgc attcacaaaa aaaagaactt tcatttccca cctcaaaact aaggaaacac
    13921 ttagacggga tttcattttt gtttagggaa tagtcgatta ccttcgctgc gctctgcgaa
    13981 caaacggcaa tgactcaaat tgaatggacc tgcactacgc catttccaaa gattggctga
    14041 aaaacaaaca cacgttcatc tcatgccgcg tctcgtcacc caatgttctt tccattttat
    14101 tgtgtcttaa tcttcccgct ttccattcct agggtgatct ttctgcatcg ttactcatgc
    14161 tccgatgcct caaacgctgt gtagctacac tcaaagtttt gatctggcta aggttcatgc
    14221 tctaaacgcc ataaaacgac ttttattatc cgctgcatga tcctgcgacc tcataggccc
    14281 agcttgtctg acttcagtgc ggttatttgt cggccataaa aataacgttt caaaacttgg
    14341 cgcatcggga taaataaaac acgcgataag atttttttaa attacaccaa aattcatgag
    14401 cataacagaa tattaaaaat aagctaccgc ccttggctct acagctacca atacactgga
    14461 agtctttcta gcggaactat gtacttatca atcacagcag ttctgcactt agccgagctg
    14521 gtttttcaca aatcaataaa tcttatgaat gttaacacaa ttctgcggga tatgcactcg
    14581 gtgatggctc gttccggccg tccgagagcc gcgcagcgca tggaggcgct tcgcgtcgga
    14641 gagctccgca gcgaagcaag ggcgcagaaa gcattacggc gtcagccgca atcccactcc
    14701 ctaccggacc gagttttcac atcgcgggca aggcctgact ggccactcag atgggagatg
    14761 gcggagtact gaagaacttc ctcacaattt taataaaaat cataatttta agtttggtgt
    14821 tcgccttgtc atgtgatcaa gtagttctgc agaaaaacaa aagtaaaaca acaagatgtg
    14881 cgaactcgat atttacacga ctctctttta ccaattctgt cctgaaattg gtagtgactc
    14941 caaaattagt gccgttgtat tattcgcgtt ttggataaga aatggaaaag attgatgtgg
    15001 tttgcctccg ttgtttcgaa aatcatgggg ttatctgcaa tggtcactcc acttcaggtg
    15061 ctcagctcta tcgctgcaag ttctgcctga aaagctttca gttctgcttt taatataact
    15121 ctactaaacc caatactcgt caagccatta ttgatatgac aatgaacggc tccggatgtc
    15181 gtgacttggc acgggttctt gatatcagct tcaatactgc ccttcaccat ctaaaaaact
    15241 ggggcctagg cagctagcac aacacgtaga tccgagagtc gaagttgtta tctgctgcga
    15301 agcggatgaa cagtggccat aagtccgttg ttaggacaat ttttgcgggt tcttctatgc
    15361 gtgcgttctt gctcatgtct ttggccctcg aaaatgaact gacgctacag caacttttgg
    15421 atctgctgag aaaatttaat attgtcgtct acatgaccga tgcctagccg gtatactgaa
    15481 cgcagctgga ttcagttcac catgtcgtta gcaaaaaata tacccaacgc atgatctgaa
    15541 tctgcgaact catcttaaaa cggcttaccc gaaggacaat ctgcttctcc aaatcagaat
    15601 aaatgcacaa taaggtcatc ggttggtata tgacaatcaa ccgttaccac tcagttgggg
    15661 gcgctatctg atttttagta aatggagcaa tgtcattcgt ctgtgattgt ttagatttaa
    15721 cctttcacac caattcttca cctctcttac aaatccccgc taggcggtac atcgtgaccg
    15781 cctttaccct gatgcctgct ctttaacaaa caggacatca gtgtatgttt aaacctttta
    15841 gcgccgaatt tttcggtact ttctggctgg ttctgggtgg ctgtggtagc gccttgatct
    15901 ctgctgcttt cccacagtta ggtataggct ttttgggcgt ggcgttggct tttggtttga
    15961 cggtagtcac catggcttat gcggtcgggc atatctccgg agcgcatttt aaccccgcgg
    16021 tgaccttggg tctgtgggcc ggtggacgct tccctgcagc gcgcgtgtta ccttacatca
    16081 tcgctcaggt tatcggcggt attgccgctg cggcagtgct gtatggtatc gccagcggta
    16141 aggctgggtt tgatgcgaca accagcggct ttgcagctaa cggctatggc attcactcac
    16201 caggcggtta tgcgttaagc gcctgtatgc tgagcgagtt tgtcctcagt gctttttttg
    16261 tcatcgtgat ccacggggcg acagaaaaac gcgcttttgc gggctttgcg ccactggcga
    16321 ttggtctgac gctgaccatc atccatctgg tgagcatccc tgtcaccaat acctcagtta
    16381 acccagcgcg cagtatcgcg gcggcggttt tccaaggtac ttgggcatta gatcagttgt
    16441 ggatgttttg cctgatccca tcattaggcg gaattgccgg tggtctgatt taccgcgcat
    16501 tgctggcgcg tccggctgaa gcataa
//
