LOCUS       MN115822               16842 bp    DNA     linear   BCT 28-JUL-2020
DEFINITION  Plesiomonas shigelloides strain O17H34 putative O antigen gene
            cluster, partial sequence.
ACCESSION   MN115822
VERSION     MN115822.1
KEYWORDS    .
SOURCE      Plesiomonas shigelloides
  ORGANISM  Plesiomonas shigelloides
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacterales incertae sedis; Plesiomonas.
REFERENCE   1  (bases 1 to 16842)
  AUTHORS   Wang,X. and Xi,D.
  TITLE     Novel O-antigen gene clusters of Plesiomonas shigelloides and their
            molecular serotyping by TaqMan MGB probes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16842)
  AUTHORS   Wang,X. and Xi,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2019) Tianjin Key Laboratory of Microbial
            Functional Genomics, TEDA College, Nankai University, 23 Hongda
            street, Tianjin 300457, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: de novo v. VelvetOptimiser v2.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16842
                     /organism="Plesiomonas shigelloides"
                     /mol_type="genomic DNA"
                     /strain="O17H34"
                     /db_xref="taxon:703"
                     /note="G5999"
     gene            1..2031
                     /gene="rep"
     CDS             1..2031
                     /gene="rep"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="QLL91058.1"
                     /translation="MRLNPRQQEAVEFVSGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIVAVTFTNKAAREMKERVTQTLGRGETKGLLVSTFHSLGLEILKREYKALGLK
                     AGFSLFDDQDQMALLKELTEDSLKGDKDLLSQLVQKISGWKNDMILPEQAKVRAKGEL
                     DILFALCYEQYARQLRACNVLDFDDLILLPTLLLRTQEEVRERWQNRIRYLLVDEYQD
                     TNTSQYELVKLLVGARARFTVVGDDDQSIYSWRGARPQNLTLLRQDFPALKVIKLEQN
                     YRSSQRILKSANILIANNPHEFEKQLFSELGPGEMLRVLQANNEEHEAERVVGEIIAH
                     RFLNKTQYKDYAILYRGNHQSRTLEKMLMQNRIPYKISGGTSFFARAEIKDIMAYLRL
                     LINPDDDTAFLRVVNTPRREIGPVTLEKLGTWAHSRGNSLFAASFDLGLEQHLHGRGL
                     EALRQFTRWIIEIADRAQRGDPVAALRDLVHGIHYESWLYETSASPKAAEMRMRNVST
                     LFGWVTEMLEGKDGEEPMTINQVVQRLTLRDMLERGEDDAEADQVQLMTLHASKGLEF
                     PHVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQKTLTFTLCKERRQYGELLR
                     PEPSRFLHELPQDDVEWEVRKAPVSAEQRQQRGRQGVAGLREMLAQAKKRPE"
     misc_feature    <1..>16842
                     /note="putative O antigen gene cluster"
     gene            complement(2186..2596)
                     /gene="rfaH"
     CDS             complement(2186..2596)
                     /gene="rfaH"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="QLL91059.1"
                     /translation="MQNIRYFCPMLTVKKQRRGQWQEEAEALFANYLFVHFNPDQLSV
                     TRVQATRGVARLVRFGETLARVPDEVLIDLARRYNPLVALPEEGKVCSQPMCTALQQA
                     LADIEQESSGEVRALRFLQLLQDHRLLASRHRVE"
     gene            complement(2940..3380)
                     /gene="c5"
     CDS             complement(2940..3380)
                     /gene="c5"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="QLL91060.1"
                     /translation="MNTVFVRAKSICAKPRQAALTLLTFLLLGVSCWVAAADMSDDAI
                     SERIKPVGGVYLADAAATAKPSGPRTGEQVYNGACIACHGSGALGAPKFRDAAQWAPR
                     LKQGIDTLQKHAIAGLNQMPARGTCTDCSDDEIKAAIEYMTSGL"
     gene            3910..5016
                     /gene="wzz"
     CDS             3910..5016
                     /gene="wzz"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz"
                     /protein_id="QLL91061.1"
                     /translation="MSKASEPQQTPYLIPQGVYPTYMPKAEDEIDLFELLGTLWKKKW
                     VILCVTLLTTGLAAVYAFTAKEQWTAKTYIQAPRIAELGSYLKFRQAYARILNQPLDT
                     NALANGLFSDLILIAESPDTKVKFLESTEYYKKETNNLSTEQDKKIWLAEQANKGLVI
                     TPPKEKGNTSYYIIQASADSAQEAYKLLQGYLNNVNNQAVTLSLDEFGQNVNTLLVNL
                     NKEIIDIDFQRKSEKLDQIAHIQRDLTTAEQAGIIDYRSSKGGFDNAQSSYKFLLGEK
                     LLSAELKATKDAPIIYPFRYYEVKRQIDELEGMLRDNIQAQAYRYQMKPSEPVIKDKP
                     NKALILILGALLGAMFAIVGTLVYATLKDKTKLD"
     gene            5052..6365
                     /gene="wbgT"
     CDS             5052..6365
                     /gene="wbgT"
                     /codon_start=1
                     /transl_table=11
                     /product="WbgT"
                     /protein_id="QLL91062.1"
                     /translation="MFYVHFIMDKKMKFDTLNAKIGIIGLGYVGLPLAVEFGKKVTTI
                     GFDINKSRIDELRNGHDSTLECSNLELLEATKLTYACSLDALKECNVFIVTVPTPIDK
                     HKQPDLTPLIKASETLGKIIKKGDVIIYESTVYPGATEEDCIPVVEKVSGLKFNIDFF
                     AGYSPERINPGDKEHRVTNILKVTSGSTPDVAEYVDQLYKLIITVGTHKASSIKVAEA
                     AKVIENTQRDVNIALINELSIIFNKLGIDTLEVLEAAGTKWNFLPFRPGLVGGHCIGV
                     DPYYLTHKAQSVGYHPEMILAGRRLNDSMGQYVVSQLVKKMLKQRIQVEGANVLVMGL
                     TFKENCPDLRNTKVIDIISELKEYNINIDIIDPWCSTDEAQHEYGLTLCEDPKVNHYD
                     AIIIAVAHNEFREMGESAIRALGKDEHVLFDLKYVLDKKSIDMRL"
     gene            6458..7483
                     /gene="wbgU"
     CDS             6458..7483
                     /gene="wbgU"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosaminuronic acid C-4 epimerase TviC"
                     /protein_id="QLL91063.1"
                     /translation="MSRYEEITQQLIFSPKTWLITGVAGFIGSNLLEKLLKLNQVVIG
                     LDNFSTGHQYNLDEVKTLVSTEQWSRFCFIEGDIRDLTTCEQVMKGVDHVLHQAALGS
                     VPRSIVDPITTNATNITGFLNILHAAKNAQVQSFTYAASSSTYGDHPALPKVEENIGN
                     PLSPYAVTKYVNEIYAQVYARTYGFKTIGLRYFNVFGRRQDPNGAYAAVIPKWTAAML
                     KGDDVYINGDGETSRDFCYIDNVIQMNILSALAKDSAKDNIYNVAVGDRTTLNELSGY
                     IYDELNLIHHIDKLSIKYREFRSGDVRHSQADVTKAIDLLQYRPNIKIREGLRLSMPW
                     YVRFLKG"
     gene            7548..8837
                     /gene="wzx"
     CDS             7548..8837
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="QLL91064.1"
                     /translation="MFILNGQCMFRKMIDAGGTFLLKAIFQIGVFVYFAHVSDITTFG
                     IISYVFTVYWFVLNFSDYGFRTKLVKDISDNSYSASELLSRSDGVKTYVFFFIFIIFM
                     FYSYVSDSISLTLLVYISSAYFVCISSGRFSLLQAVGRFRCELYINIYSTIIYIGCNL
                     FLSLFIEPLYYSAISIFIYSISLLVFSSHKCNVPCFHIKRPSLLVYKDFLDATPFAIL
                     VLLNVVLSSIDLFILKEYFSYNSVAIYQVVTRVNTGLMIVFNVIYTVLLPSFSYYLKN
                     SEWGNIRKLQRYISLLVLLLCLCYYFFGIYFVGILFGDEYKVISSATFLIMFMALIKY
                     NFWLINELYLVCSGNQSERVKSYCIGVVISIAVFFYFIPRYGWSGAVFGSAIATLVIG
                     IFYIISVKKDCGKILHDKYSLMMIFVPIFFYFIINGQ"
     gene            8921..10108
                     /gene="wzy"
     CDS             8921..10108
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="QLL91065.1"
                     /translation="MLIYLYPVLLLFNILPVFFYGQMNSDLERFFGVPIGYISDLIFY
                     FFVALTSIITLRFHVSLWTKKLLFLGIIFLIYISIQMLLLSADISGVVILLSFFSNFI
                     ALVLLVSFCIGKDELYLTHSVRNINVVMCFGIICGVVKLFIGYSEDSNFIVYLNRNAT
                     AIIVVCFYCVYSYFYRGRKSWYVSSVLYSLFFLFLDSRAGIISFAISLFFVFLQLTKK
                     EKLLISLFFVPLLTLGISFTDIGTRLERMLSSSQVIFSGGNTLTKSQNDYRRVELVFI
                     GVDVLKENYLIGTGLGVANYVKAIDKKFLGSTNFGLAHNFYLSYSAQLGIIGFILLIS
                     VFYIMLSPIFKCGGYIGKGCVFALAFYVFFNEYILTPAIYIYISIFLSVVFIRNSRMR
                     ICC"
     gene            10099..11343
                     /gene="wgbV"
     CDS             10099..11343
                     /gene="wgbV"
                     /note="plasmid"
                     /codon_start=1
                     /transl_table=11
                     /product="WbgV"
                     /protein_id="QLL91066.1"
                     /translation="MLLEYVERKISLALSKYPKVRDVIKFFYLYIASLFGIILNKNKT
                     VIQSKIYEISIDDSEESFFGYYDHSPMSSNGRYVLFHSSAFSTKRHPKKVKYISICVK
                     DLLNNKVYKLYDTRAFNWQQGSRLMWIDDDNIIFNDYENNGYISVVYSLSLMKVIKKI
                     NYPIYDVNNYKAVTLDFSWLAKYDSDYGYYNKKSFSTDISIINLNTGGIELFLSLDEM
                     LKRTNFKCNIDVEHVVNHFMFAPDGRSVMFIHRYYTPKGKRERLIHWNLINDNVRVLI
                     NESIISHCCWNGNDEIIGFFGAEIDSLNYYRLSIESCNTEKLFFDARKYSDGHPTIVH
                     NRYIISDTYPDKNRIKKLFVYDLVKNDYRELGLFYESMSFFSYSRCDLHPRISVDNRF
                     LFVDSVHSGKRKLYFMRSGICE"
     gene            11336..12238
                     /gene="wbgW"
     CDS             11336..12238
                     /gene="wbgW"
                     /codon_start=1
                     /transl_table=11
                     /product="WbgW"
                     /protein_id="QLL91067.1"
                     /translation="MSDVLVSLIIVCFNAEKYIEKSLLAFINQDVGLDKFELIIVDGD
                     SSDNTISIVQNVFSKHSNIKHKIINNKKRTLATGWNIGVLEANGKFVCRVDAHSDIPN
                     NYISKLLDDYFNIMQFDDSVVGVGGVLTNSYKTKFGSIVADFYASKFGVGNSPFRCVD
                     KNNRLKKTDTAVFALYNKDVFFDVGLFNEVLDRNQDIDFHKRVLSNNLSLYTDNSLFV
                     EYYVRDNFKDFIKKGFLDGFWVVMSGAYYFRHIVPLFFVLYLIVSFSLFFATGDYIYL
                     SFLFSYFLIFLFLFCFQFEMGEVL"
     gene            12327..13481
                     /gene="wbgX"
     CDS             12327..13481
                     /gene="wbgX"
                     /codon_start=1
                     /transl_table=11
                     /product="=glycosyltransferase"
                     /protein_id="QLL91068.1"
                     /translation="MKNFIPFALPEIGEEEIAEVIDSLRSGWITTGPKAKQFEQEFSN
                     YLGANVQSLAVNSATSGLHLALEAVGVKPGDQVIVPSYTFTATAEIVRYLGADPVIVD
                     VDRKTFNISVDAIEKAITNKTKAIIPVHFAGLACDMDSILSIAKKYDLKVVEDAAHAF
                     PTTYKGSKIGTLDSDATVFSFYANKTMTTGEGGMVVSKNKDIIERCKVMRLHGISRDA
                     FDRYQSKTPSWFYEVVAPGFKYNMPDICAAIGIHQLRKIDDFQKKRQRMAKIYDDALK
                     ELPLELPEWPTNASDIHAWHLYPIRLKTDSAINRDDFIKKLSDLGIGCSVHFIPLHKQ
                     PVWRDTYNLNASDFPVSEACYLNEISIPLYTKMTDQDQLFVIESIRQLFM"
     gene            14532..16022
                     /gene="wbgZ"
     CDS             14532..16022
                     /gene="wbgZ"
                     /codon_start=1
                     /transl_table=11
                     /product="WbgZ"
                     /protein_id="QLL91069.1"
                     /translation="MIYGAGESGRQLLPALMQAKEYFPVAFVDDNPRLHKAVIHGVTV
                     YPSDKLSYLVDRYGIKKILLAMPSVSKSQRQKVITRLEHLPCEVLSIPGMVDLVEGRA
                     QISNLKKVSIDDLLGRDPVAPDAKLMAENITGKAVMVTGAGGSIGSELCRQIVRYKPA
                     KLVLFELSEYALYAIEKELSTLCDKEGLDVSVIPLLGSVQRQNRLQMVMKSFGIQTVY
                     HAAAYKHVPLVEHNVVEGVRNNVFGTLYCAESAIDSGVETFVLISTDKAVRPTNTMGT
                     TKRLAELVLQALSARQSKTRFCMVRFGNVLGSSGSVVPLFEKQIAQGGPVTLTHRDII
                     RYFMTIPEASQLVIQAGAMGHGGDVFVLDMGDPVKIYDLAKRMIRLSGLTVRDDKNPD
                     GDIAIEVTGLRPGEKLYEELLIGDSVQGTSHPRIMTANEVMLPWQDLSLLLKELDQAC
                     HDFDHERIRSLLLQAPAAFNPTDDICDLVWQQKKSLLSQASNVIRL"
     gene            16141..16842
                     /gene="aqpZ"
     CDS             16141..16842
                     /gene="aqpZ"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="QLL91070.1"
                     /translation="MFKPFSAEFFGTFWLVLGGCGSALISAAFPQLGIGFLGVALAFG
                     LTVVTMAYAVGHISGAHFNPAVTLGLWAGGRFPAARVLPYIIAQVIGGIAAAAVLYGI
                     ASGKAGFDATTSGFAANGYGIHSPGGYALSACMLSEFVLSAFFVIVIHGATEKRAPAG
                     FAPLAIGLTLTIIHLVSIPVTNTSVNPARSIAAAVFQGTWALDQLWMFCLIPSLGGIA
                     GGLIYRALLARPAEA"
BASE COUNT     4694 a   2908 c   3744 g   5496 t
ORIGIN
        1 atgcgtttaa atccccgtca gcaggaagca gttgaatttg tcagcggacc ctgtctggtg
       61 ctggctggcg ccggctcggg aaaaacccgc gtaattacta acaaaattgc ccacctgatc
      121 cgcggctgcg gttatcaggc gcgccacatt gtggcggtga cctttaccaa caaggccgcg
      181 cgcgagatga aagagcgggt aacgcagacg ctcggtcgcg gtgaaaccaa aggcttgttg
      241 gtgtccacct tccactctct gggcttggaa atattaaagc gcgaatacaa ggcgctgggg
      301 ctgaaagccg gtttttcgct gtttgatgat caagaccaaa tggcgttgct caaagagctg
      361 accgaagaca gcctcaaagg cgataaagac ctccttagcc agttggtgca aaaaatctcc
      421 ggctggaaaa atgacatgat cttgcccgag caggctaaag tgcgcgccaa aggtgagctc
      481 gatattctgt ttgccctgtg ttatgagcag tatgcgcgcc aactacgtgc ctgcaatgtg
      541 ctggattttg acgatctgat tttgctgcca accttattat tgcgtaccca agaagaggtg
      601 cgagagcgtt ggcaaaatcg gattcgctat ttattggtgg atgagtatca agataccaac
      661 accagccagt acgagctggt gaaattgctg gtcggcgcgc gcgcccgttt caccgtggta
      721 ggggatgatg atcagtcgat ttactcatgg cgcggcgcgc gtccgcagaa tttaacctta
      781 ctgcgccaag attttccggc gttgaaagtg atcaaactgg agcagaacta ccgctcctcg
      841 cagcgcatat taaaatcggc caacatcctg attgccaaca acccacatga atttgaaaag
      901 cagctgttct ccgagctcgg tcccggtgag atgctgcgtg tgctgcaggc taacaatgaa
      961 gagcatgagg cagagcgggt ggtgggtgag attatcgcgc accgtttctt gaacaagacc
     1021 caatataaag attacgccat tttgtaccgc ggtaatcacc agtcgcgcac gctggaaaag
     1081 atgctgatgc aaaaccgcat tccgtacaag atctcgggtg gcacttcctt ctttgctcgc
     1141 gccgaaatca aagacatcat ggcctacttg cgcctgctga ttaatcctga tgatgacacc
     1201 gcgttcctgc gcgtagtgaa taccccacgg cgtgaaatcg gtccggtgac cttggaaaag
     1261 ctcgggacgt gggcgcacag tcgtggcaat agcctgtttg ccgccagctt tgatttggga
     1321 ttggagcagc acttgcatgg gcgtgggctg gaggctctgc ggcagtttac gcgttggatc
     1381 atcgagattg ccgatcgcgc ccagcgtggc gatccggtcg ccgcgctgcg tgatttggtg
     1441 catggcatcc attatgaaag ctggctctac gagacctccg ccagcccgaa agcggccgag
     1501 atgcgcatgc gcaacgtttc gaccctgttt ggctgggtga ccgagatgct ggaagggaaa
     1561 gacggcgagg agcccatgac catcaatcag gtggtgcagc gcctaacctt gcgcgacatg
     1621 ttggagcgcg gtgaagatga cgccgaagct gatcaagtgc agttaatgac gctgcacgcc
     1681 tcgaaaggac tggagtttcc gcatgtcttc ttaattggga tggaggaggg gatcttgcca
     1741 caccagacca gtttggatga agacaatgtg gaagaagaac gccgtttggc gtatgtcggg
     1801 atcactcgcg cccagaagac cttgaccttt accctatgta aagaacgccg ccagtacggt
     1861 gagttgctgc gcccagagcc gagtcgcttc ttgcacgaac tgccgcaaga tgacgtagag
     1921 tgggaagtgc gcaaagcgcc agtcagcgct gaacagcgcc agcagcgtgg ccgccaaggg
     1981 gtagccggat tacgcgagat gttggcgcag gcaaaaaaac ggcccgagtg atcttactgg
     2041 ccatgcgctt agtgtttacg catataagcg tctactcatg taagtgacta cagagataag
     2101 tgcctaatta tttaggcgct tataaatgcg gaagacgctc gcaacgggcg tgcgcacgat
     2161 gacattggaa tgccggaacg ctggattatt ccactcgatg acgggaagcc aacagacgat
     2221 gatcttgcag taattgcaaa aagcgtagcg cacgaacttc cccactgctt tcttgctcga
     2281 tatccgccaa ggcctgttgt aaggcggtac acatcggctg actacagact ttgccctctt
     2341 ccggtagtgc aaccagcgga ttgtaacgtc gcgctaaatc gatcagcacc tcatccggta
     2401 ctctcgccaa cgtttcgcca aaacgcacca aacgcgccac gccgcgcgta gcttgcactc
     2461 gcgtgacact cagctgatcg ggattaaagt gcacaaacag gtaattggca aacagtgctt
     2521 cggcctcttc ctgccattga ccgcgacgct gtttttttac cgtcagcatc ggacaaaaat
     2581 agcggatatt ttgcaactcc agatgctgtg ccgcccgact ttgctgtccg cgtttacaat
     2641 aaagaagata ccaggcgtcc atagatagtt ttgtgatgaa aataattaat gaagtatgcc
     2701 acagagtggg tagccggtca gtctttgggt gtgatgtaac agtcagttta attaatgaga
     2761 gggcagcaca aagtaccgtt cgcgcaaaat accgccggta aatcgtcagt tagagctaga
     2821 gtcatttagc gcaaacaatc atgaagagac atataaagga ggcaccgagg cctcctttta
     2881 tctatttgcc tatttgcgcg gtatttttct ttatatgctg attagcagta tcgcgtagct
     2941 tataacccgc tcgtcatgta ttcgatcgca gctttgattt catcatcact acaatcggta
     3001 caggttccgc gtgccggcat ctgatttaaa ccggcgatgg catgtttttg cagggtatcg
     3061 ataccttgct tcagacgtgg cgcccactgc gcagcatcac gaaattttgg tgcgcctaag
     3121 gctccgctgc catgacaggc gatacaggcg ccgttataca cctgctcacc ggtacgcggc
     3181 ccgctcggtt tagcggtagc cgcagcatca gctagataca ccccgccgac cggtttgatg
     3241 cgttcgctaa ttgcatcatc tgacatatcg gctgcggcaa cccagcagga tacgcccagc
     3301 agcaggaagg tcagcagagt taacgccgcc tggcgaggct tagcgcagat agacttggca
     3361 cgcacgaaga cagtgttcac agattttctc cctaaataac cagcggcttc cttttatcct
     3421 gcgttcattc ttaatcggtc tgagcagggc tgtgaaccgc agcggttgaa tattgaccac
     3481 tcggttgtgt tgccagatta taaggtaatc gtaaataaat tgaagcaatt gcgcgcgatt
     3541 cttttttgca aggccgctag cgtgcgacag cttgtacagc tccgcctcac agaatttaca
     3601 aacatactgt tgtctttttg acggcggatt tggccttcca caggcattgc tttataatct
     3661 gcactccaaa ttctgcgggc tatccgccgg tttgcaacag ggaagtggga ggtggtatat
     3721 gcctcttcac atggagtgtt ctcgatcatg tcctctaatc ccagatatca cttgttgtat
     3781 cgcagttggc gatatcctgt ttctgcgcag cgctttggga gctgaaactc aagggcggta
     3841 gcgtactttt ttgtcaggct tattcttcat ttttattttt aacccattga taaataatgg
     3901 attgatttca tgtcaaaagc atctgaacca caacagaccc cttatctgat cccgcaaggg
     3961 gtctatccaa cttatatgcc aaaagcagag gatgaaatcg atcttttcga gcttttaggc
     4021 accttgtgga agaaaaaatg ggttatttta tgtgtcacgt tgctgactac ggggttagca
     4081 gcagtgtatg cctttaccgc aaaagagcag tggacggcta aaacctatat tcaagcacca
     4141 cgtattgctg aattaggcag ctatcttaaa tttcgccaag cgtatgcccg aatattaaat
     4201 caaccgttag atacgaatgc gttggctaat gggttgtttt ccgatttgat tttgattgct
     4261 gaatcgccag acaccaaagt taaatttcta gagagtactg agtattataa aaaggaaaca
     4321 aataatttat ctactgaaca agataagaaa atttggttag ctgagcaagc gaataaaggt
     4381 cttgtgatta cgccaccaaa ggaaaaggga aatacaagtt actacataat acaagcatcg
     4441 gcagactcag cgcaagaggc atataaacta ctgcagggat atctaaataa tgttaataat
     4501 caagctgtaa cattaagtct tgatgagttt ggtcaaaatg ttaatactct tttggttaat
     4561 ctaaataaag aaattattga catagatttc cagagaaaat cagaaaagct tgatcaaata
     4621 gctcatattc agcgagattt aacaactgcg gaacaagccg gaatcattga ttatcgctct
     4681 agcaaaggcg gcttcgataa tgcgcaaagt agctataagt tcttgctcgg cgaaaaactg
     4741 ttatcagcag agctaaaagc aactaaagat gcgccaatta tttacccatt tagatattac
     4801 gaagtgaaac gtcaaattga tgagttagaa ggaatgttac gcgataacat tcaggcgcaa
     4861 gcatatcgat atcaaatgaa gccatctgag ccagttataa aagacaaacc caacaaagca
     4921 ttaattttga ttcttggtgc attactaggg gcaatgtttg ctatagttgg tacattagtt
     4981 tatgcgacat taaaagataa aaccaagtta gattaaactg ggttacgtat tgttgtgtca
     5041 atgcgaaata gatgttctat gtgcacttta taatggataa gaaaatgaaa ttcgatactt
     5101 tgaatgcgaa aattgggatt ataggccttg gttatgttgg attgcctctt gctgttgagt
     5161 ttggaaagaa agtaacgacg attggatttg atattaataa gtctcgtatt gatgaattgc
     5221 gaaatggtca cgatagtaca ttagagtgct caaatttaga gttgttagaa gcaactaaat
     5281 tgacgtacgc ctgttcatta gatgcactaa aagagtgtaa tgtatttatt gtaactgttc
     5341 caactccaat tgataaacat aaacagccag atctaacacc tctaattaaa gcatctgaaa
     5401 cattgggtaa gataataaag aaaggcgatg ttattattta tgagtcaaca gtttaccctg
     5461 gagcgacaga agaagattgt ataccagttg tagagaaagt atcaggtctt aagtttaata
     5521 ttgatttttt tgccggttat tcacctgagc gtattaatcc tggggataaa gagcatcgtg
     5581 taactaatat ccttaaggtg accagtggat ctacaccgga tgttgctgag tatgtagatc
     5641 agctatataa attaataatt actgtcggta cgcataaagc atcatcgata aaagtagcag
     5701 aggctgcaaa agtaattgaa aacacgcagc gagatgtcaa tattgcattg attaatgagt
     5761 tatctattat atttaataag ttagggattg ataccttaga ggttcttgag gctgcaggta
     5821 cgaagtggaa ttttttacct tttaggcccg gtttagtagg tggccactgt ataggtgtag
     5881 atccttatta tcttacacat aaagcgcaaa gtgtcggcta tcatccagag atgattttag
     5941 ccggacgtcg tttaaatgat agtatggggc agtatgtcgt ttcccagtta gtcaaaaaaa
     6001 tgttgaaaca acggattcaa gttgaagggg cgaatgtgtt agtgatgggg cttacattta
     6061 aagagaattg cccagatcta cgaaacacta aagtgattga tattatttca gagttaaaag
     6121 aatacaatat caatatagat attatagatc catggtgttc taccgatgag gcacaacatg
     6181 aatatggatt aactttatgt gaagatccta aagttaatca ttatgatgca ataattatcg
     6241 ctgttgcaca caatgagttt cgcgagatgg gagagagcgc tattcgtgca ttaggtaaag
     6301 acgagcacgt tttgttcgat ttaaaatatg tgcttgataa aaaaagtatc gatatgcgct
     6361 tgtaagagtg attaaaaaaa tcaaatcctc tttgatatga tacacctcag cattttatgc
     6421 taggtttagc acttgattaa tatacatgga tatttatatg tctcgctatg aagagattac
     6481 acagcagtta attttttcac cgaaaacttg gttaattact ggtgtcgctg gctttatagg
     6541 atcaaatctt ttagaaaagt tacttaaatt aaaccaggtt gttattgggt tagataactt
     6601 ttccacggga catcaatata atcttgatga agttaaaaca ttagtttcca ctgaacagtg
     6661 gagtcgattt tgctttatag aaggtgatat tcgagatctc actacctgtg agcaagttat
     6721 gaaaggtgtt gatcatgtct tacatcaggc tgcgctaggt tctgtacctc gttcaattgt
     6781 tgatcctata acaaccaatg caactaatat tactggattt ttgaatatct tacatgcggc
     6841 taaaaatgca caagtacaaa gttttactta tgctgcatca agctcaactt atggagatca
     6901 tcccgcacta ccaaaagtag aggaaaacat tggtaatcca ctttctcctt atgcagttac
     6961 taaatatgtt aacgagattt atgctcaggt atatgctcga acatatggtt ttaaaactat
     7021 tggattacgt tattttaatg tatttggtcg tcgtcaagat cctaatggag cttatgctgc
     7081 agtaattcca aaatggacag cagcaatgct taaaggtgat gacgtatata ttaatggcga
     7141 tggtgaaacg agtcgtgatt tttgttatat agataatgtt atacaaatga atatattatc
     7201 tgcattagcg aaggacagtg ctaaagataa tatatataat gttgcagttg gtgatagaac
     7261 aacattaaat gaattatctg gttacattta tgatgagctt aatttaattc accatatcga
     7321 taaattgagc attaagtata gagagtttag atctggagat gttaggcatt ctcaggctga
     7381 tgttactaag gctatagatt tactacagta tagaccaaat ataaaaatca gagagggatt
     7441 acgactttca atgccgtggt atgtgagatt tttaaaaggc taaattatat taacatgaat
     7501 aaataatcta tttcacctct gttattaatg caggggtgaa aatctatgtg tttattctaa
     7561 atggtcagtg tatgtttaga aaaatgattg atgcaggtgg tacattttta cttaaagcaa
     7621 tatttcaaat aggagttttt gtttatttcg cacatgtgtc agatattact acatttggta
     7681 ttattagtta tgtgtttact gtttattggt ttgtgcttaa cttctctgat tatggattta
     7741 gaacaaaatt agtgaaagat atttctgata atagttattc tgcatcagaa ttattatcca
     7801 gaagtgatgg agttaaaaca tatgtttttt tcttcatttt tataatcttc atgttttatt
     7861 cttatgtttc tgattcaatt tcattaactc tgcttgttta tatttcatct gcatattttg
     7921 tttgtatttc aagtggtaga tttagcttgc tacaggctgt tggtcggttt agatgtgaat
     7981 tatatataaa tatctactca acaattatat atattgggtg taatttattt ttatctctgt
     8041 ttatcgaacc tctatattat agtgcgatat caatattcat atactcaatt tcgcttttgg
     8101 ttttctcatc acataaatgc aatgtgccat gttttcatat aaaaagacca agtcttttag
     8161 tttataaaga ttttttggat gcaactccgt tcgctattct ggtgttacta aatgttgttt
     8221 tatctagtat tgaccttttt atattaaaag aatatttctc ttataatagt gttgctatat
     8281 atcaggtggt aactagggtt aataccggtc taatgatagt gtttaatgtt atttatactg
     8341 ttttattgcc ttcattttct tattatctga aaaattctga atggggtaat ataaggaaat
     8401 tacaacgata tatatcactg ttagtcttat tactatgttt atgctattat ttttttggca
     8461 tctatttcgt agggatattg tttggtgatg agtataaggt aatatcttct gcaacatttt
     8521 tgataatgtt tatggctctt attaaatata atttttggct aataaatgaa ctttatcttg
     8581 tgtgtagtgg aaatcaaagc gagcgagtta aatcgtattg tattggtgtg gtcatttcaa
     8641 tagcggtttt cttttatttt atacctcggt atggatggag tggggcggtt tttggaagtg
     8701 ccattgcaac attagtaatt ggaatatttt atattatttc tgtgaaaaaa gattgtggga
     8761 aaattcttca tgataagtat tcactaatga tgatctttgt cccaattttc ttttatttta
     8821 ttattaatgg tcagtagcgg ttgttatatt aatctgttgt tgttttatat cgttccatta
     8881 atatgtttag actcgattgg aagtctaata aaggttaagt atgttaatat acctatatcc
     8941 tgtacttttg ttatttaata tccttccggt ttttttttat ggacaaatga actctgattt
     9001 agagcgtttt tttggagttc ctattggcta tatttcagat ttaatatttt atttctttgt
     9061 tgctttaaca tctataataa cgttgaggtt tcacgtttct ctgtggacaa agaaattatt
     9121 atttttaggc atcatattcc tgatttatat cagcattcag atgttgttgt tatcagcgga
     9181 tatctcaggt gtcgtaattt tattatcgtt tttttctaat tttatagctt tggttctttt
     9241 ggtatcattt tgcattggta aagatgagct ttatttaact cattcggtta gaaatataaa
     9301 tgttgtaatg tgttttggta ttatctgtgg agttgtaaaa ttatttattg gttattctga
     9361 agatagtaat tttatagttt atttaaatag aaatgccacc gcaattatag tagtgtgctt
     9421 ttattgtgta tattcatact tttatcgtgg tcgaaagtct tggtatgtct catctgtatt
     9481 gtactctctg ttctttcttt ttctagatag ccgagcagga ataatatcat ttgctatatc
     9541 gttgtttttt gtttttcttc agttaacaaa gaaggaaaag ttattaatat cattgttttt
     9601 tgttcctctt ctaactttag gtatttcttt tactgatata ggcactcgtc ttgaacgaat
     9661 gctgtcttcg tcacaggtta tattctctgg tggtaacact cttacaaaaa gtcagaatga
     9721 ttatcgtcga gttgagttag tatttattgg ggttgatgtt ttaaaagaaa attatttaat
     9781 tggcactgga ttaggtgttg caaattatgt aaaggctata gataaaaagt ttttaggaag
     9841 taccaacttt gggttggcgc ataattttta tttatcttat tcggctcagt tagggattat
     9901 tggttttatt ttgcttattt ctgtatttta tataatgctg tctccaattt ttaaatgcgg
     9961 agggtatatt ggtaaagggt gcgtttttgc tttggctttc tatgtctttt ttaatgagta
    10021 tatattgacg ccagcgatat atatttatat ttctattttt ttatcggtgg tttttatacg
    10081 taattctagg atgagaatat gttgttagaa tatgttgaaa gaaaaatttc cttagccttg
    10141 agtaagtatc ctaaggtaag ggatgttatt aagttctttt atttatatat cgcatcatta
    10201 ttcggaatta ttttgaataa aaataagacg gttattcaat caaaaatata cgagatttca
    10261 attgatgatt ctgaagaatc attttttggc tattatgacc atagtccaat gagctctaat
    10321 gggcggtacg tattgttcca ctctagtgcg tttagcacta aacgacaccc aaagaaagtt
    10381 aagtatatat ctatttgcgt aaaagacctt cttaataaca aagtttataa gctatatgat
    10441 acgcgagcat ttaattggca gcagggaagc cgattaatgt ggattgatga tgacaatata
    10501 atttttaatg actatgaaaa taatggatac attagtgttg tctattcttt gtctttgatg
    10561 aaggttataa aaaaaataaa ctatccgatt tatgatgtga ataattacaa ggctgtgacg
    10621 ttagatttct catggctggc taaatatgat agcgattatg gttattataa taaaaaatca
    10681 ttttctacag atatttcaat cattaatttg aacacgggcg gaatagaatt atttttatcc
    10741 ttagacgaaa tgctaaagag aactaatttt aaatgtaata ttgatgttga acatgtggtc
    10801 aatcatttta tgtttgctcc cgatggacgt tccgttatgt tcatacatcg atactataca
    10861 cctaaaggaa agcgtgaaag gttaatacat tggaatttaa taaatgataa tgttcgagtc
    10921 ctaataaatg aatcgattat tagtcattgt tgttggaatg ggaatgatga aattataggt
    10981 ttttttggtg cagaaataga ttcgcttaat tattatagat tgtcaattga atcctgtaat
    11041 acagagaaat tgttttttga tgcaagaaaa tattctgatg gacatcctac tatagttcat
    11101 aatagatata ttatatctga tacttaccca gataaaaata gaattaaaaa gttgtttgtt
    11161 tatgaccttg tcaaaaatga ttatcgcgag cttggattgt tttatgagtc aatgagtttt
    11221 ttttcttatt ctcgatgtga cttacatcca aggatctcgg ttgataatag atttttgttt
    11281 gttgattcag ttcactcagg gaaaagaaaa ctatatttta tgaggagtgg tatttgtgag
    11341 tgatgttcta gtatctttaa ttatagtttg ctttaatgca gagaagtata ttgaaaaatc
    11401 tcttttggca tttattaatc aagatgttgg attagataaa tttgaattga ttattgtaga
    11461 tggggattca tctgataata caatatctat tgttcagaat gttttttcta aacatagtaa
    11521 cattaagcat aaaattatca ataataaaaa aagaactctt gctacgggtt ggaatattgg
    11581 ggtgctagaa gctaatggta agtttgtgtg tagagttgat gcacatagtg atattccaaa
    11641 taactatata tctaaattat tagatgatta ttttaatatt atgcagtttg atgatagcgt
    11701 tgttggtgtt ggaggtgtat taactaattc ttataaaact aagtttggtt caattgtagc
    11761 ggatttttat gcatcgaaat ttggtgttgg taattctcca tttaggtgcg tagacaaaaa
    11821 taatcgacta aaaaaaacag atacggctgt ctttgcttta tataataaag atgtgttttt
    11881 tgatgttgga ctttttaatg aagtattaga tagaaatcaa gatattgatt ttcataagag
    11941 agttttaagc aataatttgt cattatatac agataatagt ttatttgttg agtattatgt
    12001 tagagataat tttaaagatt tcataaagaa aggttttctt gatggttttt gggttgttat
    12061 gtctggagca tattatttta gacatatcgt gccacttttt tttgttttgt atttaattgt
    12121 atctttttct cttttctttg ctactggtga ttatatatat ttatctttct tattttctta
    12181 ttttcttatt ttcttatttc tattttgttt tcaattcgag atgggcgaag ttttataggt
    12241 aaagtatttc ttccttttat atttttgtct tatcatattt cttatggatg tggatcgtta
    12301 ttatcttttt tgaaaaggta ttttaaatga aaaattttat tccttttgcg ttacctgaaa
    12361 ttggcgaaga agaaattgca gaggtaattg actctttacg ttcaggttgg attacgacag
    12421 gtcctaaggc taagcaattt gaacaagaat tttctaatta cctaggagcg aacgttcaat
    12481 cattagctgt taactctgct acgtcgggct tacatttggc tcttgaagct gttggcgtaa
    12541 aacctggaga ccaagttatt gtcccatcat atacattcac tgctactgcc gaaattgtca
    12601 ggtaccttgg tgctgatcct gtaattgttg atgtagatcg taaaacattt aatatatcag
    12661 ttgatgccat tgagaaggct attactaata aaacaaaggc gattattcca gtacacttcg
    12721 ctggattagc ttgtgacatg gattcaatct tatcaattgc taaaaaatat gacctaaagg
    12781 ttgtcgagga tgccgctcat gcatttccta caacatataa aggaagtaag ataggaacgc
    12841 ttgattcaga tgctacggtt tttagcttct acgccaataa aactatgaca accggtgaag
    12901 gcggaatggt tgtttcaaaa aataaagata taattgagcg ttgtaaggta atgcgtttac
    12961 atggaatcag tcgtgacgct tttgaccggt accagtctaa aactccttct tggttttatg
    13021 aggttgtagc tccagggttt aaatacaata tgcctgatat ctgtgcggca atcggtattc
    13081 atcaacttag aaagatcgat gattttcaga aaaaacgtca acgaatggca aaaatttacg
    13141 atgatgcgtt aaaagaattg ccacttgaat tgcctgaatg gcctactaat gctagtgata
    13201 ttcatgcttg gcatctatat cctatccgct taaaaactga ttcggctatt aatcgcgatg
    13261 attttattaa gaagttatca gatcttggaa ttggttgttc tgtccatttt ataccgttgc
    13321 ataagcaacc ggtttggcgt gatacatata atttgaacgc cagtgacttt ccagtttctg
    13381 aggcgtgtta tttaaatgaa atatctattc ctctttatac taaaatgacg gatcaagatc
    13441 agttgttcgt tatcgaatcg attagacaat tatttatgta atggtatttt atattaaatg
    13501 aaacgtattt ttgatgttat cgtggcaggc ttaggcctgc tttttctatt tcctgttttt
    13561 atcattgtgt caatgttaat tgttgctgat tctaaaggga gtgttttttt taggcagtat
    13621 agagttggga gatttgggaa agattttagg atacataaat ttagaacgat gtttatcgat
    13681 tcagaaaaaa aaggacggat aacagttggt caagatgctc gggtaaccag agttggatgg
    13741 tatttacgga agtacaaaat cgatgagctg cctcaattga tagatgttct ttctggaaca
    13801 atgagtttgg ttggcccaag accggaagtg agggagttta ttgatgagta tcctgatgat
    13861 ataagggaaa aagttttatc ggttaggcca gggataactg acttagcatc tatagaaatg
    13921 gtagatgaaa atgagatttt gtctagttat gatgacccac gtagggctta tatagatata
    13981 attcttccaa tcaagcaaag atattatttg gattatgttg ctaacaattc agtaaagtat
    14041 gattgtgtga taatttggaa aactcttatt aagattttgt cgcgataata aggtagtgta
    14101 ggatgattga tagaatattg gagctgccaa gaattgttaa gagaggtatc atcatctgca
    14161 ttgatgtagt tatggtgata ttctcatttt ggttgtctta ttggttgagg cttgatgagc
    14221 aaacggcttt tcttagtgca ccgatgtggt ttgctgcagc tattcttacc atatttaccg
    14281 tgtttatatt tatcaggatt gggctttatc gggcagtctt acggtatgtt agtgcaaaga
    14341 taatgttgct aatatcagtt ggtattctgg cctcaacgtt atctcttgtc gttatatcat
    14401 attcgctatc cataatgttg ccgcgcactg ttgtcggaat ttattttttg gttttacttt
    14461 tactgacatc aggctctaga ttgcttttta gaatgatact taactatgga gttaagggta
    14521 gtgcgcctgt tttgatttat ggcgctggtg aatctggccg acaattattg ccagcattaa
    14581 tgcaggcaaa agaatatttt cctgtggcat ttgtggatga taatcctcgc ttgcataagg
    14641 ccgtcattca tggtgtaaca gtttatccct cggataaact gagttaccta gtagatcgct
    14701 atggtataaa gaaaattctt ttggcgatgc cgagcgtcag taagtcacaa aggcagaaag
    14761 tgattactcg tttagagcat ttaccgtgtg aagttctctc tattccgggc atggtcgatt
    14821 tagtcgaagg tcgagcacaa atcagtaatc tcaaaaaagt atcgattgat gacttgctag
    14881 gccgtgatcc agttgctcct gatgccaaat tgatggcgga gaacattaca ggcaaagcag
    14941 ttatggtcac tggggcggga ggatcgatcg gctctgagct ttgtcgtcaa attgttcgat
    15001 ataagccagc caaattggtt ctatttgaac tgtctgaata tgccctgtat gccattgaga
    15061 aagagctatc gacgctgtgt gataaagaag gtttggatgt ctcagtgatc cctctgttgg
    15121 gctcggtgca gcgtcagaat cgcttacaga tggtgatgaa gtcctttggt attcaaacgg
    15181 tttatcatgc ggctgcttat aaacatgtgc ctctggttga gcataatgtg gtggaagggg
    15241 tgcgtaataa tgtgtttggt accttgtact gcgctgagtc ggcgatcgat agtggcgttg
    15301 aaacctttgt gttgatttcc accgataaag cggtgcggcc gaccaacact atggggacaa
    15361 ccaagcgcct ggctgagttg gtattgcagg cgttgtctgc acggcaaagc aaaacccgtt
    15421 tttgtatggt gcgatttggt aatgtgctgg gatcctcggg ctcagttgta ccattgtttg
    15481 aaaagcagat tgcccaaggt gggccagtta ccctgactca tcgtgacatt attcgctatt
    15541 ttatgacaat tcctgaagca tcgcagttgg tgattcaagc gggggcgatg gggcatggcg
    15601 gcgatgtctt tgtcttagac atgggcgatc cggttaagat ttatgactta gccaaacgca
    15661 tgatccggtt aagtggcttg actgtgcggg atgataaaaa tccagatggc gatattgcca
    15721 ttgaagttac gggattacgt ccaggtgaga aactgtatga agaattactg attggtgatt
    15781 cagttcaagg tacctctcat ccacgaatta tgacggccaa cgaagtgatg ctaccgtggc
    15841 aggatctatc gctcttactt aaagagctgg atcaagcctg tcatgacttt gatcatgagc
    15901 gcattcgcag cttattgtta caagcaccag cggcattcaa tccaactgat gatatttgcg
    15961 atctagtttg gcagcagaaa aaatcgctgt tatcacaagc gagcaatgtc atacgcctgt
    16021 gattgtttag atttaacctt ccacaccaat tcttcacctc tcttacaaat ccccgctagg
    16081 cggttcatcg tgaccgcctt taccctgatg tcagctcttt aacaaacagg acatcagtgt
    16141 atgtttaaac cttttagcgc cgaatttttc ggtactttct ggctggttct gggtggctgt
    16201 ggtagcgcct tgatctctgc tgctttccct cagttaggta ttggcttttt gggcgtggcg
    16261 ttggcttttg gtctgacagt agtcaccatg gcttatgcgg tcgggcatat ctccggagcg
    16321 cattttaacc ccgcggtgac cttgggtctg tgggccggtg gacgcttccc tgcggcgcgc
    16381 gtgttacctt acatcatcgc tcaggttatc ggcggtattg ccgctgcggc agtgctgtat
    16441 ggtatcgcca gcggtaaggc ggggtttgat gcgacaacca gcggctttgc agctaatggc
    16501 tatggcattc actcaccagg cggttatgcg ttaagcgcct gtatgctgag cgagtttgtc
    16561 ctcagtgcgt tttttgtcat cgtgatccac ggggcgacag aaaaacgcgc tcctgcgggc
    16621 tttgcgccgt tggcgattgg tctgacgctg accatcattc atttggtgag catccctgtc
    16681 accaatacct cggttaaccc tgcgcgtagt atcgcggcgg cagttttcca aggtacttgg
    16741 gcgttagatc agttgtggat gttttgcttg atcccatcat taggcggaat tgccggtggt
    16801 ctgatttacc gcgcattgct ggcgcgtccg gctgaagcat aa
//
