LOCUS       MN115823               16858 bp    DNA     linear   BCT 28-JUL-2020
DEFINITION  Plesiomonas shigelloides strain O18H2 putative O antigen gene
            cluster, partial sequence.
ACCESSION   MN115823
VERSION     MN115823.1
KEYWORDS    .
SOURCE      Plesiomonas shigelloides
  ORGANISM  Plesiomonas shigelloides
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacterales incertae sedis; Plesiomonas.
REFERENCE   1  (bases 1 to 16858)
  AUTHORS   Wang,X. and Xi,D.
  TITLE     Novel O-antigen gene clusters of Plesiomonas shigelloides and their
            molecular serotyping by TaqMan MGB probes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16858)
  AUTHORS   Wang,X. and Xi,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2019) Tianjin Key Laboratory of Microbial
            Functional Genomics, TEDA College, Nankai University, 23 Hongda
            street, Tianjin 300457, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: de novo v. VelvetOptimiser v2.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16858
                     /organism="Plesiomonas shigelloides"
                     /mol_type="genomic DNA"
                     /strain="O18H2"
                     /db_xref="taxon:703"
                     /note="G6000"
     gene            1..2031
                     /gene="rep"
     CDS             1..2031
                     /gene="rep"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="QLL91071.1"
                     /translation="MRLNPRQQEAVEFVSGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIVAVTFTNKAAREMKERVAQTLGRGETKGLLVSTFHSLGLEILKREYKALGLK
                     AGFSLFDDQDQMALLKELTEDCLKGDKDLLSQLVQKISGWKNDMILPEQAKVRAKGER
                     DILFALCYEQYARQLRACNVLDFDDLILLPTLLLRTQEEVRERWQNRIRYLLVDEYQD
                     TNTSQYELVKLLVGARARFTVVGDDDQSIYSWRGARPQNLTLLRQDFPALKVIKLEQN
                     YRSSQRILKSANILIANNPHEFEKQLFSELGPGEMLRVLQANNEEHEAERVVGEIIAH
                     RFLNKTQYKDYAILYRGNHQSRTLEKMLMQNRIPYKISGGTSFFARAEIKDIMAYLRL
                     LINPDDDTAFLRVVNTPRREIGPVTLEKLGTWAHSRGNSLFAASFDLGLEQHLQGRGL
                     EALRQFTRWITEIADRAQRGDPVAALRDLVHGIHYESWLYETSASPKAAEMRMRNVST
                     LFGWVTEMLEGKDGEEPMTINQVVQRLTLRDMLERGEDDAEADQVQLMTLHASKGLEF
                     PHVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQKTLTFTLCKERRQYGELLR
                     PEPSRFLHELPQDDLEWEVRKAPVSAEQRQQRGRQGVAGLREMLAQAKKRPE"
     misc_feature    <1..>16858
                     /note="putative O antigen gene cluster"
     gene            complement(2185..2595)
                     /gene="rfaH"
     CDS             complement(2185..2595)
                     /gene="rfaH"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="QLL91072.1"
                     /translation="MQNIRYFCPMLTVKKQRRGQLQEEAEALFANYLFVHFNPDQLSV
                     TRVQATRGVARLVRFGETLARVPDEVLIDLARRYNPLVALPEEGKVCSQPMCTALQQA
                     LADIERESSGEVRALRFLQLLQDHRLLASRHRVE"
     gene            2705..2821
                     /gene="orf03"
     CDS             2705..2821
                     /gene="orf03"
                     /codon_start=1
                     /transl_table=11
                     /product="Orf03"
                     /protein_id="QLL91073.1"
                     /translation="MDSRSVFGCDVTVSLINEVLHKVPFAQNTAGKSSARAS"
     gene            complement(2948..3337)
                     /gene="c5"
     CDS             complement(2948..3337)
                     /gene="c5"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="QLL91074.1"
                     /translation="MALTLLTSLLLGVSCWVAAADMSDDAISERIKPVGGVYLADAAA
                     TAKPSGPRTGEQVYNGACIACHGSGALGAPKFRDAAQWAPRLKQGIDTLQKHAIAGLN
                     QMPARGTCTDCSDDEIKAAIEYMTSGL"
     gene            complement(3439..3558)
                     /gene="orf05"
     CDS             complement(3439..3558)
                     /gene="orf05"
                     /codon_start=1
                     /transl_table=11
                     /product="Orf05"
                     /protein_id="QLL91075.1"
                     /translation="MKKESGAIASIYLRLPYNLATQPSGQHSTAAVHRPAQTD"
     gene            3917..5020
                     /gene="wzz"
     CDS             3917..5020
                     /gene="wzz"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz"
                     /protein_id="QLL91076.1"
                     /translation="MSKASEPQQTPYLIPQGVYPTYMPKAEDEIDLFELLGTLWKKKW
                     VILCVTLLTTGLAAVYAFTAKEQWTAKTYIQAPRIAELGSYLKFRQAYARILNQPLDT
                     GALANGLFSDLILIAESPDTKFKFLENTEYYKKETQSLSSEQDKKIWLTEQANKGLVI
                     TPPKEKENISYYTIQASADSAQEAYKLLQGYLKDVNNQAVTLSLDEFDQNINTLLVSL
                     KKEVNDIDFQKKAEKLDQIAYIQRDLTTAEQAGITDYRSSKNGFDNAQSSYKFLLGEK
                     LLSAELKATKDAPIIYPFRYYEVKRQIDELEGMLRDNIQAQAYRYQMLPTEPLKKDKP
                     KKALILVLGAFLGGMLGITGVLIQSVISSRKDT"
     gene            5094..6047
                     /gene="galE"
     CDS             5094..6047
                     /gene="galE"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="QLL91077.1"
                     /translation="MRVAIIGGSGFIGSVLSKALANKKIAFSILDKNKSSIFPEYWTY
                     CDVTKLDTLESSLREHDVIINLAAEHKDNVSPSDLYYQVNVDGARNVCCAAEKLGINK
                     IIFTSSVAVYGFVDKDTDETGEFHPFNHYGKSKLEAEQVYDQWQAASPTDRVLTTIRP
                     TVVFGEGNRGNVYNLFRQIASGKFLMIGKGNNPKSMAYVENVVAFLTHCLSLSTGRKV
                     FNYVDKPDFDMLSLANLVNDSLGKKTNSIKIPYLLGLSAGYCFDLLANISGKEFPVSS
                     IRVRKFCARTQFSSSSISETGFVAPVSLHDAVIKTVRHEFS"
     gene            6079..6978
                     /gene="cpsC"
     CDS             6079..6978
                     /gene="cpsC"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="QLL91078.1"
                     /translation="MNNSVVYVYCLEQDKEKIQQAIKNYPVNVCFNCFDFDSYNDKTL
                     LHYKSGLLDEKVTELLIHATKKGAHVEPLVSYLDSRFGHTEVELLHSGYFLHMKAFSI
                     LSRRSNRVIKRILDLFFVALLSVIAIPVGLITAIAIKKESPGPVFYRQKRVGQYNQEF
                     EVVKFRSMRADAEKNGAQWASKNDSRVTRIGNFIRKTRIDELPQLINVLKGEMSMVGP
                     RPEREVFIKELETVIPYYRFRHAVKPGITGLAQVKYPYGASIEDAVWKHKYDIYYIKH
                     QSFFMDVKILMLTVRTVLFGMGR"
     gene            6975..7991
                     /gene="gt"
     CDS             6975..7991
                     /gene="gt1"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="QLL91079.1"
                     /translation="MKTVIFIITKSEVGGAQRWVYEQAKIISNDCKVILVTSEDGWLT
                     KQTVFSEVKLLPGIAKLIRPLELFKLIKIIKQNSAFSVVASSANAGVYARLARLFVAF
                     KCVYVSHGWSCLYNGGSLKKVFCKVEKYLSFLTDTIWCISKSDRDKAVNIIGIKENKI
                     TMSLNSITPLKPRGDADFHGRVLFVGRLTYPKRPDLIADVVARDKRYSLDIIGDGDLY
                     PELYRLYGECDNISFLGEIAEFRDFNKYDVFVLTSDSEGLPMSALEAASAGVPMILSN
                     VGGCFEVINGENGILVKNEALDISEALNIVADNYAYYLDNAIKYRREFDISAKAHEYK
                     CIIL"
     gene            8235..8810
                     /gene="IS3"
     CDS             8235..8810
                     /gene="IS3"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="QLL91080.1"
                     /translation="MSNTNTNFEMTGILLGQEVRKRKTPLEKIAIIQQTMEPGMNVSH
                     VARLHGIQPSLLFKWNKQYQEGSLTAVSTGEEVVPASELTAALKQVRELQRLLGKKTM
                     EVEILKEAVECGHSRKWMAHAPLLLKDGEIALVSRTIGVSRAQLSLRIKRSADWQDRR
                     SHLRNDEADAEILSDILDIISDMPSYGYRRV"
     gene            10899..11063
                     /gene="IS1"
     CDS             10899..11063
                     /gene="IS1"
                     /note="iso-IS1 ORF1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLL91081.1"
                     /translation="MFQLDYSYEARKPGIKEQITEMAFNGAGVRDTARTLKVGINTVI
                     RTLKNSRHDE"
     gene            11821..13065
                     /gene="wzx"
     CDS             11821..13065
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="QLL91082.1"
                     /translation="MSSTVRNGLSLLVMQGVNYLIPLMTLPYLTRVLGAYQYGVVNTV
                     LSFGAYAILVIDFGFNLTATKDVSIANSRGDIEKIFSRVLISKAILFFISFLLVFLFV
                     NLISSYDGIEYLIYFLIPQLLGSVFFPVWLFQGIGKVHFISIITSITKALTVPLMFLF
                     VNGPDDMAVCLLIMGGPLFVSGIIGIYLSYRVGIKFNLGFVSFSSIKNTLHSSLPVFI
                     GNVAIGLYTACTPIILSLVSNYNEVGYYTAADKLRAAILGVFLVLGQVIYPRANYLRY
                     HDVNEFGRFIRRLFLCQLFFGTIAALLFYVFMPRLAPFILGSGFDMLDDIVKLMAPMI
                     LLIPLSVIMCNCIILPNGNTKAFAFLPIVTAFLHIPYAIYLSHYYGAVGGTISILITE
                     VISFLIIMSYCIKKNYFKLIFK"
     gene            13076..13846
                     /gene="gt"
     CDS             13076..13846
                     /gene="gt2"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="QLL91083.1"
                     /translation="MHFKTFVISLSDAKDRRNSIATQLKKINMDFEIVDAVDLRNETS
                     SIVDEVISNYEVDTLRPMTRGEIGCSLSHKKAYGKIIDSNFDFGLILEDDALINESLY
                     SVLNNLLVNDLMESDVLILGYSKLSMSDSNLFYIKEPILKKIHIDGYSFGSVWREWTC
                     GTVGYLLSRRAAEKMLKSPIISIADDWDTHKRVFNLDIIHCRPGLIFEDYENMSSSIE
                     SERLTFVSKEFVLYKVARIIRGQCRMVLMKILDLFNVN"
     gene            13836..14897
                     /gene="wzy"
     CDS             13836..14897
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="QLL91084.1"
                     /translation="MLTNASILFLAFVLLLKPQFYHTTENAIIIQMLSVITFCYYSFF
                     SGGVGRSYFFFGMIFLFVLFPLTILFKITACYPMVVGILCVFERRRSFPVRDNEKAAL
                     LLGVCFLFSFLLYQSHLGRYSLLNGDPNYTSLIFLTLILVAHYISNSHLLKAFSVLFV
                     FFIVFMTESRTAVIAVLAYFICSKIKCKRFFSLSIFIFSIVSQYFSAYVFPRFISSME
                     LGENRFFDLNDTSNHERIMIYKQAVDYIGEHFYFFLSRGVTDYLAVNLNATNIPHNWF
                     VQSIIGFGLIFTLVYSIVMMFYIATTKHEHCYVLPFMCFFMIFGAFLSYYSLTSPLLV
                     LCLMFPLISSSGFNIKRGV"
     gene            14927..15982
                     /gene="gt"
     CDS             14927..15982
                     /gene="gt3"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="QLL91085.1"
                     /translation="MGGAERQVCELADQFYIAGHTVLIISMSGDTVTQPKSAGVRIVS
                     LRMKKGLLNFFSNFRMARTIVRDFNPDVVHSHMVHANLFARLLRLTVSMRKLICTAHN
                     TNEGGLIRMLSYRITDSLCDISTNVSQEAVDAFVLHKAVPANRMLPMHNGIDTDRFLF
                     DSSMRKDLRKKLNLTDCDSLILAVGRLNVQKDYPNLLTAFSYIVSANTEIQLAVIGIG
                     EEESVLTSMVDELGLNKHVHFLGLQHNVNEWLSAADVFVLSSAWEGFGLVVAEAMACK
                     RVVVATDCGGVKEVLGDCGFLVPPQNSQALADGLSAALSLTSDEARVKGELARQRVIE
                     RYSLQEQAKRWLELYQR"
     gene            16157..16858
                     /gene="aqpZ"
     CDS             16157..16858
                     /gene="aqpZ"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="QLL91086.1"
                     /translation="MFKPFSAEFFGTFWLVLGGCGSALISAAFPQLGIGFLGVALAFG
                     LTVVTMAYAVGHISGAHFNPAVTLGLWAGGRFPAARVLPYIIAQVIGGIAAAAVLYGI
                     ASGKAGFDATTSGFAANGYGIHSPGGYALSACMLSEFVLSAFFVIVIHGATEKRAPAG
                     FAPLAIGLTLTIIHLVSIPVTNTSVNPARSIAAAVFQGTWALDQLWMFCLIPSLGGIA
                     GGLIYRALLVRPAEA"
BASE COUNT     4590 a   3207 c   3997 g   5064 t
ORIGIN
        1 atgcgtttaa atccccgtca gcaggaagcg gttgaatttg tcagcggacc ctgtctggtg
       61 ctggctggcg ccggctcggg aaaaacccgc gtaattacca acaaaattgc ccatctgatc
      121 cgcggctgcg gttatcaggc gcgccatatt gtggcggtga cctttaccaa caaggccgcg
      181 cgtgagatga aagagcgggt agcgcagacg ctcggtcgcg gtgaaaccaa aggcttgttg
      241 gtgtccacct tccactctct gggcttggaa atcttaaagc gcgaatacaa agcactgggg
      301 ctgaaagccg gtttttcgct gtttgacgat caagaccaaa tggcgctgct caaagagctg
      361 accgaagact gcctcaaagg ggataaagac ctcctcagcc agctggtgca aaaaatctcc
      421 ggctggaaaa atgacatgat cttgcccgag caggccaaag tgcgcgccaa aggtgagcgc
      481 gatatcttgt ttgccctgtg ctatgagcag tatgcgcgcc agctacgcgc ctgcaatgtg
      541 ctggatttcg acgatctgat tttgctgccg accttattat tgcgcaccca agaagaggtg
      601 cgagagcgtt ggcaaaatcg cattcgctat ttattggtgg atgagtatca agataccaac
      661 accagccagt acgagctggt gaaattgctg gtcggcgcgc gcgcgcgttt caccgtggta
      721 ggggatgatg atcagtcgat ttactcatgg cgcggtgcgc ggccacagaa cttgaccttg
      781 ctgcgccagg attttccggc gttgaaagtg atcaagctgg agcagaacta ccgctcctcg
      841 cagcgcatat taaaatcggc caacatcttg attgccaata acccgcatga gtttgaaaag
      901 caactgttct ccgagctcgg ccccggtgag atgctgcgtg tgctgcaggc taacaacgaa
      961 gagcatgagg ctgagcgcgt ggtcggcgag atcatcgcac accgtttctt gaacaagacc
     1021 caatataaag attacgccat tttgtaccgc ggtaatcacc agtcgcgcac gctggaaaag
     1081 atgctgatgc aaaaccgcat tccgtacaag atctcgggtg gcacttcttt ctttgcccgc
     1141 gccgaaatca aagacatcat ggcctacttg cgcctgctga ttaatcccga tgatgacacc
     1201 gcgttcctgc gcgtcgtgaa taccccacgg cgtgaaatcg gtccggtgac cttggaaaag
     1261 ctcggtacat gggcgcacag tcgtggcaat agcctgtttg ccgccagctt tgatttaggc
     1321 ctcgagcagc acttgcaggg gcgtgggctg gaagcattgc ggcagtttac gcgttggatc
     1381 accgagattg ccgatcgcgc ccagcgtggc gatccggtcg ccgcgctgcg tgatttggtg
     1441 catggcatcc attatgaaag ctggctctac gagacctccg ccagcccgaa agcggctgag
     1501 atgcgcatgc gcaacgtctc gaccctgttt gggtgggtaa ccgagatgct ggaagggaaa
     1561 gacggcgagg agccgatgac catcaatcag gtggtgcaac gcctcacctt gcgcgacatg
     1621 ttggagcgcg gcgaagatga cgccgaagcc gatcaagtgc agttaatgac gctgcacgca
     1681 tcgaaaggac tggagtttcc gcatgtcttc ttaattggga tggaagaggg gatcttgccg
     1741 caccagacca gtttggatga agacaatgtg gaagaagagc gccgcttggc gtatgtcggg
     1801 atcacccgcg cccagaagac cttgaccttt accctatgta aagagcgccg ccagtacggt
     1861 gagttgttgc gcccagagcc gagtcgcttc ttgcacgaac tgccacaaga tgacttagaa
     1921 tgggaagtgc gcaaggcgcc agtcagcgcc gaacagcgtc agcagcgcgg ccgccaaggg
     1981 gtagctggat tacgcgagat gttggcgcag gcgaaaaaac ggcccgagtg atcttactgg
     2041 catgggctta gtatttacgc atataagtgt ctactcatat aagtgactac agagataagt
     2101 gcctaattat ttaggcgctt ataaatgcgg aagacgctcg caacgggcgt gcgcactatg
     2161 acattggaat gccgaaacgc tggattattc cactcggtga cgggaagcca acagacggtg
     2221 atcttgcagt aattgcaaaa agcggagtgc acgaacttca ccactgcttt cacgctcgat
     2281 atctgccaag gcctgttgta aggcggtaca catcggctga ctacagactt taccctcttc
     2341 cggtagtgca accagcgggt tgtaacgtcg cgctaaatcg atcagcacct catccggcac
     2401 ccttgccaac gtttcgccaa aacgcaccaa tcgcgccacg ccgcgcgtag cttgcactcg
     2461 agtgacactc agctgatcgg gattaaagtg cacaaacagg taattggcaa acagcgcttc
     2521 ggcctcttcc tgcaattgac ctcgacgctg tttttttacc gtcagcatcg gacaaaaata
     2581 gcggatattt tgcaactcca gatgttgcgc cgctcgactt tgctgtccgc gtttacaata
     2641 aagaagatac caggcgtcca tagatagttt tgtgatgaaa ataattaatg cagtatgcca
     2701 cagagtggat agccggtcag tctttgggtg tgatgtaaca gtcagtttaa ttaacgaagt
     2761 gctgcacaaa gtaccgttcg cgcaaaatac cgccggtaaa tcgtcagcta gagccagtta
     2821 agccagagtc atttagtgca aacaatcatg aagagacata taaaggaggc accgaggcct
     2881 ccttttatct atttgcctat ttgcgcggta tttttcttta atatgctgat tagcagtatc
     2941 gcgtagctta taacccgctg gtcatgtatt cgatcgcggc tttgatttca tcatcactac
     3001 aatcggtaca ggttccgcgt gccggcatct gatttaaacc ggcgatagca tgtttttgca
     3061 gggtatcgat accttgcttc agacgtggcg cccactgcgc ggcatcacga aattttggtg
     3121 cgcctaaggc gccactgcca tgacaggcga tacaggcgcc gttatatact tgctcaccgg
     3181 tacgtggccc actaggttta gcggtggccg ctgcatcagc cagatatacc ccgccgaccg
     3241 gtttgatacg ttcgctaatc gcatcatccg acatatcagc cgcggcaacc caacaggata
     3301 cgcccagcag cagggaggtc agcagagtta atgccacctg gcgaggctta gcgcagatag
     3361 acttggcacg cacaaagaca gtattcacag attttctccc taaataacca gcggcatcct
     3421 tttatcctgc tttcattctt aatcggtctg agcagggcgg tgaaccgcag cggttgaatg
     3481 ttgaccactc ggttgtgttg ccagattata aggtaatcgt aaataaattg aagcaattgc
     3541 gccggattct tttttcaagg ccgctagcgt gcgacagctt gtacagccca gcctcacaga
     3601 atttacaaac atactgttat ctttttgacg gcggatttgg ccttacacag gcattgcttt
     3661 ataatctgca ctccaaattc tgcgggctat ccgctggttt gcaacaggga agtgggaggc
     3721 ggtatatgcc tcttcacatg gagtgttctc gatcatgtcc tctaatccca gatatcactt
     3781 gttgtatcgc agttggcgat atcctgtttc tgcgcagcgc tttgggagct gaaactcaag
     3841 ggcggtagcg tgcttttttg tcaggcttat tcttcatttt tatttttaac ccattgataa
     3901 ataatggatt gatttcatgt caaaagcatc tgaaccacaa cagacccctt atctgatccc
     3961 gcaaggggtc tatccaactt atatgccaaa agcagaggat gaaatcgatc ttttcgagct
     4021 tttaggcacc ttgtggaaga aaaaatgggt tattttatgt gtcacgttgc tgactacggg
     4081 gttagcagca gtgtatgcct ttacagcaaa agaacagtgg acggctaaaa cctatattca
     4141 agcaccacgt attgctgaat tagggagtta ccttaaattt cgtcaagcgt atgctagaat
     4201 tttaaatcaa ccgttagata caggtgcgct ggctaatgga ttgttttccg atttgatttt
     4261 gatcgctgaa tcaccagaca ctaaatttaa attcctagag aacacagagt attataaaaa
     4321 agaaacacag agtttatcct ctgagcaaga taagaaaatt tggttaactg agcaagcgaa
     4381 taaaggcctt gtgattacgc caccaaagga aaaggaaaat ataagttact acacaataca
     4441 agcatcggca gattcagcgc aagaggcata taaactacta cagggttacc taaaggatgt
     4501 taataatcaa gctgtaacat taagtcttga tgagtttgat caaaacatca acactctttt
     4561 agttagttta aagaaagaag ttaatgatat cgatttccag aaaaaagcag aaaaactgga
     4621 ccagatagcc tatatccagc gagatttaac tacagcagag caagcgggta ttactgatta
     4681 tcgctctagt aaaaatggat ttgataatgc gcaaagtagc tataagttct tgcttggtga
     4741 aaaacttctg tcagcagagc tgaaagcaac taaagatgct cctattattt atccatttag
     4801 atattatgaa gtgaaacgtc aaattgatga gttagaaggc atgttgcgcg ataatattca
     4861 ggcgcaagca tatcgttatc agatgttgcc gacagaaccg ttgaaaaaag ataaacctaa
     4921 aaaagctctg attttggtac taggggcgtt cttggggggc atgttaggaa ttacaggtgt
     4981 attgattcag tcagtgattt catctcgaaa agacacttag tcttgggatc tgacatcaat
     5041 actataaaag tcactgtcga gttgtagcaa attaaggtat agggaataat ctaatgagag
     5101 ttgcaatcat cggtggttct ggttttattg gtagtgtctt atctaaggcg ctggctaata
     5161 aaaaaatagc attttcgatt ttagataaaa acaaaagttc aattttccca gaatattgga
     5221 catattgtga tgtgacaaag ctcgatacgt tggaatctag tttgcgtgag catgacgtca
     5281 taatcaacct cgcggctgaa cataaagata acgttagtcc atcagattta tattatcaag
     5341 ttaatgttga tggagccaga aatgtttgtt gtgcagcaga aaaattaggt ataaataaga
     5401 tcatctttac ttcatcagtt gctgtgtatg gttttgttga taaagatacg gatgaaacag
     5461 gagagtttca tccgttcaat cattatggaa aatcgaagtt agaagctgaa caggtatatg
     5521 accagtggca ggccgcatca ccaactgata gagttttaac aaccattcga cctaccgttg
     5581 tgtttggcga agggaaccga ggtaacgtat ataatttatt taggcaaatt gcctcgggaa
     5641 aatttttgat gataggcaaa ggaaataatc ctaagtctat ggcgtatgtt gagaatgttg
     5701 ttgcctttct aactcattgc ctgagtctgt ctacagggcg aaaagtattt aattatgtag
     5761 ataaacctga ctttgatatg ctctccttag ctaacttagt taacgatagt ctaggaaaaa
     5821 aaacaaatag tatcaagatt ccctatctac taggtttgtc agcgggttac tgctttgact
     5881 tgttagcaaa cataagcgga aaagaatttc ctgtcagcag tattcgggtg cgaaagtttt
     5941 gtgctagaac tcaatttagc tcttcgagta taagtgaaac agggtttgtt gcaccagtat
     6001 cattgcatga tgctgtgatt aaaactgtta gacatgaatt ttcctaaata accaaaggta
     6061 aagtggtttt agattagtat gaataattct gtagtgtatg tttattgtct cgaacaagat
     6121 aaagagaaaa ttcagcaagc aataaaaaat tatccagtta acgtttgttt taattgtttt
     6181 gattttgata gttataatga taaaacatta ctgcattata aaagcggtct tcttgatgag
     6241 aaggttactg agttactaat tcatgccact aagaaagggg cgcatgtaga gccattggtt
     6301 agttatttag actctcgctt tggtcacact gaagttgagt tattgcatag tggttatttt
     6361 ttgcacatga aggcgttttc gattctatcg cgtcgtagca atcgagttat taaacgcatt
     6421 ctagatctat tttttgttgc attgctcagt gtaatagcaa tacctgtagg gcttattacc
     6481 gcgattgcga ttaaaaaaga gtctccaggc ccagtatttt atcgacaaaa gcgagttggc
     6541 cagtacaatc aagaatttga agttgtcaaa tttagatcaa tgagggctga tgcagagaaa
     6601 aatggtgcgc agtgggcaag caaaaatgat agtcgtgtta ctcgtattgg taactttatc
     6661 cggaaaactc gaattgatga gttaccacag ctcattaatg tattgaaagg tgagatgtcg
     6721 atggttggcc cccgccctga gcgagaggtc tttattaaag aactagagac ggttatccct
     6781 tactatcgtt ttcgacacgc agtgaagccg gggattacag ggttagctca ggtaaagtat
     6841 ccatatggcg catcaattga ggatgctgtt tggaagcata agtatgacat ttactatata
     6901 aagcatcaga gttttttcat ggatgttaag atattgatgt taaccgtaag aacagtttta
     6961 tttgggatgg ggcgatgaag accgttattt tcattataac taaatctgaa gttggtggag
     7021 ctcagcgttg ggtttatgag caggcaaaaa taatatctaa tgattgcaaa gtgattttag
     7081 ttacgtctga agatggttgg ttgactaagc agaccgtatt ttctgaagtg aagttgcttc
     7141 ctggaatagc aaagctaata agaccacttg agttattcaa attaataaaa ataattaaac
     7201 agaattctgc attttcggtg gtcgccagtt cagcaaatgc gggggtatat gctaggttgg
     7261 ctagattatt tgttgcgttt aagtgtgttt acgtatcgca tgggtggtca tgtttatata
     7321 atggcggttc actaaagaag gttttttgta aagtagaaaa atatctttca tttttgaccg
     7381 atacaatttg gtgtatttca aaaagtgata gagataaggc tgttaatatt ataggaataa
     7441 aggaaaataa aatcacaatg tcattaaatt ctattacgcc attaaagcca aggggagatg
     7501 ctgattttca tgggcgggta ttatttgtcg gtcgactaac ttaccctaaa agacctgatt
     7561 taattgctga tgtagttgct cgtgataaga gatatagctt ggatatcatt ggtgatggtg
     7621 atctttatcc tgaattgtat aggctctacg gagaatgtga taatatttca tttttaggtg
     7681 agattgctga atttagagat tttaataaat atgacgtatt tgttttaaca tccgatagtg
     7741 aggggttgcc aatgtctgct ttagaggcgg catctgcggg ggtacccatg attctcagta
     7801 atgtaggtgg gtgttttgag gttattaatg gtgaaaatgg gattttagta aaaaacgaag
     7861 ctttagatat aagtgaggct ctaaatattg tcgcggataa ttatgcttac tatttagata
     7921 atgcaattaa atataggagg gagtttgata tatcagctaa agctcatgag tacaagtgca
     7981 tcatattgta attttggtta ataattctaa attagtgccg ttgtattgtt cgtcatttaa
     8041 ataataaatg ggaaagttta ttttattttc ccacattgcg ccaaaactca ggagtattcg
     8101 taatgggcgc tctacttgag gcgttcaatt ctatcgatgc aacctctgct tgaaaagatt
     8161 ttagttcagc tgatgtagcc ccctgaaaca tcagacagta gctgtatctc cagataagag
     8221 ataggcttga atatatgtct aacactaaca ccaattttga gatgaccggg atcctgttag
     8281 ggcaagaagt ccgtaaacgt aaaactcctt tggagaagat cgccattatc cagcagacga
     8341 tggagccggg catgaatgtc tcccatgtcg cccgcctgca tggtatccag cccagcctgc
     8401 tgtttaagtg gaataagcaa tatcaggaag gcagcctaac cgccgtttcg actggagagg
     8461 aagtcgttcc tgcttctgag cttactgctg cgttgaagca ggttcgggag cttcagcgtc
     8521 tcctgggcaa gaagacgatg gaagtagaga tcctgaagga agccgtggag tgcggtcatt
     8581 cgcgaaaatg gatggcgcac gcgcccttgt tgctaaagga cggggaaata gccttggtca
     8641 gccgcaccat aggtgtgtca cgtgcgcagt tgtcactgcg gattaaacgt tctgctgact
     8701 ggcaggatag gcgcagtcac ttgcgtaatg acgaagcaga cgctgaaata ctgtcggaca
     8761 ttctcgacat catcagcgat atgccgagtt atggataccg ccgtgtgtag gggtatcctc
     8821 cgtcagcaac gccgcacaga aggaatgtca ccggtgaatg ccaaacggct ttaccgaata
     8881 atgagcgaac ataacctgtt gctacggaat gacaaaccag agcggccaaa acgtgggcat
     8941 aagagtcaaa tcgcgataac agaaagcgat acgcgctggt gttcagatgg ctttgagttc
     9001 ggctgcgaca acggtgaaaa attgcgggta acgttcgcac tggactgctg cgacagggag
     9061 gctacgatac agttcaaccg tggaggatgt gatgctgagg tcggtagaaa agcgcttcgg
     9121 cgacaggttg ccggacatac cagtacaatg gctgacggat aacggttcag tgtatactgc
     9181 gcatgaaacg aggaagttcg ccagagagct gaacttaaag ccctgtacaa cagcggtgag
     9241 tagtccacag agtaatagca tggctgaacg gttcgtgaaa acgatgaagg aagactatat
     9301 cgcgttcatg ccaaaaccgg atgtgagaac agcactgcga aaccttgcag cagcgttcac
     9361 gcattacaat gaaaagcact cgcacagcgc gctgggatat aactccccga gagaatactg
     9421 gcggtagcga gcatcattaa cttaagctac aaaagctgtc cggagatggc gagtcaagat
     9481 caaagatttt agctcagctt tcaatctgaa tctaatatcc accaatatat tattgaaatg
     9541 gcaatgaacg actccaaata tcgtgacata gtacgaattc ttgcttatgt atttgattct
     9601 cgaaatgcat tgacctgaca acagcttctt acgtagctaa gaaaatttaa tcttgccttc
     9661 tacatgtcta catgattgat gcttggctgg tatatcgaac gaagctagat tcagcttacc
     9721 atgtagttag ctagggtgtg tcccgtaact attttcttta caattcagat ctcttttctc
     9781 gataagaagt gttcaaattt ggcttgctat gatgttccag atgaagcgtg gacgctcatc
     9841 tccccaattt taccgcctga gcgcaactta ataacaggtg gacgacctta tcttgaacat
     9901 cgacccgtga tgaatggtat tttttgggtg ctttgttcgg gagcaccttg gcgagtatgg
     9961 ttattggaaa accatttata atcgtttcaa tcgatggcca aaaccagggg taattagcat
    10021 gattttcaat cggttacttc atgttctaga tgataaaaaa cttatcgatt gggacgctat
    10081 tgcccttgat ggaagtaata ttcgggcgtt aaaagctgcc gcgggtgcta aaaaaatatc
    10141 ccgatgacct cagtaatcat ggggtgggtc gctctcgagg cggctttggt accaaaatcc
    10201 acttggcgac tgattgtaca ggattaccac tgagtttatg catcagtgcg gggtaagcac
    10261 atgaaagcca atacgcagag tatttactca atagaattgg cattatgcgt aaaaatggtc
    10321 atctaaaatt tcaccctaaa tcggtacttg cacgataaag gatactcaag cagtagactt
    10381 cataactatc tgaaaattaa tagtataaaa gctgttgtac ccttcaaatc caacgagaga
    10441 gcgggtcgag acaaacgtag aaaactgaat gttaagttgt acaaaaggcg aaatgtcgtt
    10501 gagcggtgct ttgcgatgtt aaaagaatat cgtcgaatag cgactcgctc agaaaaaacg
    10561 aaaacggcaa ggaattatct agcaatgctc aaattagggt cgatcagatt gtttttgaag
    10621 cgcttaaagg ggttacggga cacaccctag tcagcgaaaa atacacctaa ggtatagggt
    10681 gccataactt gagtctgcgc attcatcttg aattgcttac tcgaaggggc aacgcctcca
    10741 attggttgaa ttcggtgtgt ggtgtataat gtttattttg aggttcaccc atggccagca
    10801 ttatcgttca ttgtccccgt tgtcagtctg ctctggttta tcgtcatgga cagaacccga
    10861 aaggatatga ccgattccgc tgccgagagt gccaccgcgt gtttcaactc gattactcat
    10921 acgaggcccg caagccgggg attaaagagc agatcactga aatggccttc aacggtgcgg
    10981 gagttcgcga taccgctcgg acactgaaag taggtatcaa tactgtcatc cggactttaa
    11041 aaaactctcg tcacgacgaa taacatcgtc tccggtggcg catgctgatg tggcgcttat
    11101 ctgtgagctt gacgaacaat ggagctttat cggtagtaag gcgcgtcagc actggctctg
    11161 gtacgcgtac aacacaaaaa cgggtggcgt actggcctac acttttggtc cacgaactga
    11221 tgcaacttgc cgtgaattac tggccctgct cctcccttta acatcggaat gctaaccaca
    11281 gatgaatggg gcagttatgc tagagagctt cctaaagaga aacacttgac cggcaagatc
    11341 tttactcaac gcattgagca caacaatcta actctgagaa cgcgaatcaa gcgtctagct
    11401 cgcaaaacga tctgcttctc tcgctctgtt gaacttcatg aaaaagtcat tggcgctttt
    11461 atcgaaaaat gtatgttcta ctaattggat gcattaccct cgaaggacaa acttgtctca
    11521 caattagata tgcacgataa ggtcatcggt tgatgtataa caattaatca ttaatttagc
    11581 gttacgacat caaattgaca taaatatatg gcaagtgttg tttatctctc gtgtgttaat
    11641 attgttgcct ggaggaatta tattaatttt tagtgcgtga cagagtttgt gtggcgtgat
    11701 ggctacattt aattctcgct ttaagtaatc tgtaaaagca gatggcttaa aaaattagtt
    11761 ttctctagga gatctccttg tctattggag tgttatttat tttgttgcag aataatgtat
    11821 atgtctagta cagttagaaa tgggctatct ttgttggtga tgcaaggcgt taactattta
    11881 atccctttga tgacgttacc ttatttgact cgagtgttag gtgcctatca atacggagtt
    11941 gtaaatactg ttctaagctt tggtgcatat gctatactgg tcatagactt cggtttcaac
    12001 cttacagcaa caaaagatgt atctatagct aatagtagag gtgatataga gaaaattttt
    12061 tctcgagttc tgatttctaa ggcgatttta tttttcattt ctttcttgtt ggttttttta
    12121 tttgttaatt taatttcaag ctatgatgga atagagtatt taatatattt tttaatccct
    12181 caactgcttg gtagtgtttt ttttcctgtt tggctattcc aaggtattgg taaagtacac
    12241 tttatttcaa taattacatc catcacaaaa gcattaacag ttccattaat gtttttgttt
    12301 gtgaatgggc cggatgatat ggctgtttgt ttattaatta tgggagggcc attattcgtc
    12361 tctggaatta ttgggattta tttgtcttat agggttggaa taaagtttaa tcttgggttt
    12421 gtgagttttt catcaataaa aaatacattg catagttcac tccctgtatt tataggtaat
    12481 gttgctatag gtctttatac agcatgtacg ccaataattt taagcttggt tagtaattat
    12541 aatgaggtag ggtattatac agcggcagat aaattacgtg ctgctatttt gggggttttt
    12601 ttagttttag ggcaggttat ttatcctaga gctaattatt tacgttacca tgatgtgaat
    12661 gagtttggac gatttattag acgtttattt ttatgccaac tctttttcgg gacgattgct
    12721 gctttattgt tttatgtctt tatgcctcgg ctggctccgt ttattttagg ttctggcttt
    12781 gacatgcttg atgatattgt aaaattaatg gcgccaatga tactacttat accgctatcg
    12841 gttataatgt gcaactgtat tattcttcct aatggaaata cgaaggcctt tgcttttctt
    12901 cctattgtga ccgcatttct acatattcca tacgcgattt atttaagtca ttattacggt
    12961 gctgttggcg gaactatatc catattaatt acagaggtga ttagctttct tataatcatg
    13021 tcttactgta ttaagaaaaa ttactttaag ctaatattta aatgaggtgg gaattttgca
    13081 ttttaaaaca tttgtgattt ctttgagtga tgctaaagat agacgcaatt caatagctac
    13141 tcagttaaaa aaaattaaca tggattttga aattgttgat gcagtagacc ttcgcaatga
    13201 aactagctct atagtagatg aagtgatctc taattatgag gttgatacgt taagaccgat
    13261 gactagaggc gaaattggtt gctctttaag tcacaaaaaa gcttatggga aaatcattga
    13321 ctcaaatttt gattttggcc tcattcttga ggatgatgca ttaataaatg aatcattgta
    13381 ttcggtttta aataatttgc tggttaatga cttaatggaa agtgatgtgt taattctagg
    13441 ctactccaag ctatcaatgt cagatagtaa tttattctat attaaagaac cgattttaaa
    13501 aaaaattcat attgatgggt attcttttgg tagtgtttgg cgagagtgga catgtggaac
    13561 tgttggatat ctcctaagta ggagagcggc agagaaaatg ttaaaatcgc caattatttc
    13621 tattgctgat gattgggata cacacaagag agtgtttaat cttgatatta ttcattgtag
    13681 gcctgggttg atttttgaag attatgagaa tatgagcagc agcatcgaaa gtgaaagact
    13741 tactttcgtg agtaaagagt ttgttttata taaggttgct cgcattatta gagggcagtg
    13801 tagaatggta ttgatgaaaa tattggattt atttaatgtt aactaatgcg tccattttat
    13861 ttctcgcttt tgtgttgcta ttaaaaccgc agttttacca cactacagag aatgcaatta
    13921 ttatccaaat gctatctgta ataacttttt gttattattc gtttttttct ggaggcgtag
    13981 ggagatctta tttttttttc ggtatgattt ttttatttgt tttgtttccg ttgacaattt
    14041 tatttaagat tacagcttgt tacccaatgg ttgtggggat cctatgtgtg tttgagagac
    14101 gacgtagttt ccctgtcaga gataatgaaa aagcggcttt attattgggg gtgtgctttt
    14161 tattttcatt tctactctat cagtcacacc tcggtagata ttcgttgcta aatggtgatc
    14221 caaattatac tagcctgatt tttttgacgc taattcttgt ggctcattac atatcaaata
    14281 gtcatttgct aaaggcattc agtgttttat ttgttttttt tattgttttc atgactgaat
    14341 cgagaactgc cgtgatcgct gttcttgctt actttatttg cagtaaaatt aagtgtaagc
    14401 gttttttttc gctttccatt tttatttttt ctatcgtctc ccagtacttc tctgcttatg
    14461 tgttccctcg gtttatatct agtatggagt tgggcgaaaa taggtttttt gatctaaatg
    14521 ataccagtaa tcatgaaaga ataatgattt ataaacaagc cgttgactat atcggtgaac
    14581 atttttactt tttcttgtct agaggtgtga cggattattt ggctgttaat ttgaatgcta
    14641 ctaacatacc gcacaattgg tttgttcaat caataattgg gttcggttta atatttacgt
    14701 tggtgtattc aattgtaatg atgttttata tagcaacaac aaagcatgag cattgttatg
    14761 ttttaccatt tatgtgtttt tttatgattt ttggggcttt tcttagctat tactcactta
    14821 catcaccatt gttagttttg tgtttgatgt ttccactgat ttcaagttcc ggttttaata
    14881 ttaagagggg tgtatgaaga ttttattttt cattactggt ttaggaatgg gaggggctga
    14941 gagacaggtc tgtgaactcg cagatcaatt ttatattgct ggacatactg tgcttattat
    15001 ttctatgtca ggagatacag tgactcagcc taagtcagct ggcgtgagaa ttgtttcatt
    15061 gagaatgaaa aaggggttgt tgaatttttt ttcgaatttt aggatggcta gaactatcgt
    15121 aagagatttt aatcctgatg tggtgcatag ccatatggtt catgctaatt tgtttgctcg
    15181 tttgttgaga ttaactgtga gcatgcgaaa attaatttgt actgcccata atactaatga
    15241 gggagggctg attagaatgc tttcttaccg cattactgat agcttgtgcg atattagtac
    15301 aaatgttagt caggaagcgg ttgatgcatt tgtgttacat aaagctgtgc cagctaatcg
    15361 aatgctgcct atgcataatg gtattgatac tgatcgtttt ttatttgatt catcaatgcg
    15421 taaagatcta agaaagaaac taaatttaac agattgtgac tcgttgattt tggcggttgg
    15481 acgactaaat gttcaaaaag attatccaaa tttattaact gcttttagtt atatagtctc
    15541 tgcgaatact gaaattcaat tggctgtaat tggtatcggt gaagaagaat cagttttaac
    15601 atctatggtg gatgagcttg ggctaaataa acatgtccat tttttaggcc ttcagcataa
    15661 cgtcaatgaa tggcttagtg ctgccgatgt gtttgtcctt tcctctgcgt gggaaggctt
    15721 tggattagtc gttgcagagg cgatggcatg taagcgagtc gtagtggcta ccgattgtgg
    15781 gggggttaaa gaggttttag gtgattgtgg tttcttagtc cctccccaaa atagccaggc
    15841 attagcggat ggtttatcgg ctgctctttc attgacgagt gacgaggcga gagtaaaagg
    15901 tgaattagct cggcagagag tgatcgagcg ttattcattg caggagcaag caaaacgttg
    15961 gctagagtta tatcaacgct gagtgttgtt ttctctggat tatgtatatg ctgttttgag
    16021 cgttttattg gcgtatcagt taatggcttt aaccttccac accaattctt cacctctctt
    16081 acaaatcccc gctaggcggt acatcgtgac cgcctttacc ctgatgcctg ctctttaaca
    16141 aacaggacat cagtgtatgt ttaaaccttt tagcgccgaa tttttcggca ctttctggct
    16201 ggttctgggt ggctgtggta gcgccttgat ctctgctgct ttccctcagt taggcattgg
    16261 ctttttgggc gtggcgttgg cttttggtct gacagtagtc accatggctt atgcggtcgg
    16321 gcatatctcc ggagcgcatt ttaaccccgc ggtgaccttg ggtctgtggg ccggtggacg
    16381 cttccctgca gcgcgtgtgt taccttacat catcgcgcag gttatcggcg gtattgccgc
    16441 tgcggcagtg ctgtatggta tcgccagcgg taaggcgggg tttgatgcga caaccagcgg
    16501 ttttgcggct aatggctatg gaatccattc tcctggcggc tatgcgttaa gcgcctgtat
    16561 gctgagcgag tttgtcctca gtgcgttttt tgtcatcgtg atccacgggg cgacagaaaa
    16621 acgggctcct gcgggctttg cgccactggc gattggtctg acgctgacca tcatccatct
    16681 ggtgagcatc cctgtcacca atacctcagt taacccggcg cgtagtatcg cggcggcggt
    16741 tttccaaggt acttgggcgt tagatcagtt gtggatgttt tgcttgatcc catcattagg
    16801 cggaattgcc ggtggtctga tttaccgtgc attgctggtg cgtccggctg aagcataa
//
