LOCUS       MK551182               15506 bp    DNA     linear   BCT 05-MAY-2019
DEFINITION  Plesiomonas shigelloides strain O23H1a1c putative O antigen gene
            cluster, complete sequence.
ACCESSION   MK551182
VERSION     MK551182.1
KEYWORDS    .
SOURCE      Plesiomonas shigelloides
  ORGANISM  Plesiomonas shigelloides
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacterales incertae sedis; Plesiomonas.
REFERENCE   1  (bases 1 to 15506)
  AUTHORS   Xi,D., Wang,X., Ning,K., Liu,Q., Jing,F., Guo,X. and Cao,B.
  TITLE     O-Antigen Gene Clusters of Plesiomonas shigelloides Serogroups and
            Its Application in Development of a Molecular Serotyping Scheme
  JOURNAL   Front Microbiol 10 (2019) In press
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15506)
  AUTHORS   Xi,D., Wang,X., Ning,K., Liu,Q., Jing,F., Guo,X. and Cao,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2019) TEDA, Nankai University, Hongda Street 23,
            Tianjin 300457, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: VelvetOptimiser v. v2.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15506
                     /organism="Plesiomonas shigelloides"
                     /mol_type="genomic DNA"
                     /strain="O23H1a1c"
                     /db_xref="taxon:703"
     gene            1..2031
                     /gene="rep"
     CDS             1..2031
                     /gene="rep"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="QCH03160.1"
                     /translation="MRLNPRQQEAVEFVSGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIVAVTFTNKAAREMKERVAQTLGRGETKGLLVSTFHSLGLEILKREYKALGLK
                     AGFSLFDDQDQMALLKELTEDSLKGDKDLLSQLVQKISGWKNDMILPEQAKVRAKGER
                     DILFALCYEQYARQLRACNVLDFDDLILLPTLLLRTQEEVRERWQNRIRYLLVDEYQD
                     TNTSQYELVKLLVGARARFTVVGDDDQSIYSWRGARPQNLTLLRQDFPALKVIKLEQN
                     YRSSQRILKSANILIANNPHEFEKQLFSELGPGEMLRVLQANNEEHEAERVVGEIIAH
                     RFLNKTQYKDYAILYRGNHQSRTLEKMLMQNRIPYKISGGTSFFARAEIKDIMAYLRL
                     LINPDDDTAFLRVVNTPRREIGPVTLEKLGTWAHSRGNSLFAASFDLGLEQHLQGRGL
                     EALRQFTRWITEIADRAQRGDPVAALRDLVHGIHYESWLYETSASPKAAEMRMRNVST
                     LFGWVTEMLEGKDGEEPMTINQVVQRLTLRDMLERGEDDAEADQVQLMTLHASKGLEF
                     PHVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQKTLTFTLCKERRQYGELLR
                     PEPSRFLHELPQDDLEWEVRKAPVSAEQRQQRGRQGVAGLREMLAQAKKRPE"
     misc_feature    1..15506
                     /note="putative O antigen gene cluster"
     gene            complement(2187..2597)
                     /gene="rfaH"
     CDS             complement(2187..2597)
                     /gene="rfaH"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="QCH03161.1"
                     /translation="MQNIRYFCPMLTVTKQRRGKTQEESEALFANYLFVHFNPDQLSV
                     TRVQATRGVARLVRFGETLARVPDEVLIDLARRYNPLVALPEEGKVCSQPMCTALQQA
                     LVDIERESNGEVRALRFLQLLQDHRLLASRHRVE"
     gene            complement(2934..3359)
                     /gene="c5"
     CDS             complement(2934..3359)
                     /gene="c5"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5"
                     /protein_id="QCH03162.1"
                     /translation="MRAKSICAKPRQAALTLLTSLLLGVSCWAAAADMSDDAISERIK
                     PVGGVYLADAAATAKPSGPRTGEQVYNGACIACHSSGALGAPKFRDAAQWAPRLKQGI
                     DTLQKHAIAGLNQMPARGTCTDCSDDEIKAAIEYMTSGL"
     gene            3732..3878
                     /gene="orf4"
     CDS             3732..3878
                     /gene="orf4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QCH03163.1"
                     /translation="MFSIMPSIPDITFIVIGDILFCFCTALWELKLKGGSVLFSRFIL
                     HFYF"
     gene            3906..5024
                     /gene="wzz"
     CDS             3906..5024
                     /gene="wzz"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz"
                     /protein_id="QCH03164.1"
                     /translation="MSKASEPQQTPYLIPQGAYPTYMPKAEDEIDLFELLGTLWKKKW
                     VILFVTLLTTGLAAVYAFTAKEQWTAKTYIQAPRIAELGSYLKFRQAYARILNQSLDT
                     GALARGLFSDLILLAESPDTKASFLSHTEYYKKETADLSSETEKKIWLDNQINKSIVI
                     SPPDEKKGSNYYTVEFSADSAKNAYQLLQAYLKDVNGQAVTLNLSEFDQNINALLVNL
                     KKEVQDIDFKKKAEKLDQIAFIQRDLTTAEQAGITDYRANKNGFDTAQNSYKFLLGEK
                     LLSAELKSVKDTPIIYPYRYYEIKRQVEELEGMLRDNIQAQAFRYQMAPIEPLKKDKP
                     KKALLLVLGALLGGMFGVAGVLVQSVIINRKRINKIAQ"
     gene            5099..6115
                     /gene="wecA"
     CDS             5099..6115
                     /gene="wecA"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="QCH03165.1"
                     /translation="MTTWLAILVLRPTSQRIGLVDVPNARKLHVGAVPLVGGIAVSFG
                     VLMATLFGLIPQNPMISILLLGGVGMVIMGAIDDAKDISPWLRLFFQALLIALVCYYG
                     NTTLHNFGNLFNFGNIKIPTAIAWPVTILAVCAAINAYNMMDGIDGLAGCMAAISLAA
                     LGYTLYSVEPDLSRLCWVIVIALIPYLMVNLTIPPFSRKIFLGDAGSMFLGFIIGVLV
                     LYGSQQSNGHNAFNPITALWFIGLPLMDMLGIMVRRIRKGQSPLRADRNHLHHILLHA
                     GLSPKQSLLLMVLINVLIVTCGILLESSGAPQWLMVTLYLVLFMTYSLCLAYACKLVR
                     VNDI"
     gene            6461..7537
                     /gene="mnaA"
     CDS             6461..7537
                     /gene="mnaA"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     non-hydrolyzing"
                     /protein_id="QCH03166.1"
                     /translation="MAPLVHALAKDERFEARVCVTAQHREMLDQVLDLFEIKPDYDFN
                     LMKVGQTLNEVTAKILLELKPVLQEFKPDVVLVHGDTATTFAASLAAYYEQIAVGHVE
                     AGLRTGNIYSPWPEEANRKLTGALTRYHFAPTETARQNLLQENYAPENIYVTGNTVID
                     ALLMVKAKIDANPVLKTILATQFPYLDEKRKLILVTGHRRESFGDGFKRICEALSLIA
                     KKYPDVQIVYPVHLNPNVQKPVNRLLSGFDNVYLIEPQEYLPFVYLMNRSYLILTDSG
                     GIQEEAPSLGKPVLVMRETTERPEAVDAGTVTLVGTCVKTICYKAFWLLENEYVYSKM
                     SKSHNPYGDGNAVIKITDAIYSNR"
     gene            7543..8601
                     /gene="gt"
     CDS             7543..8601
                     /gene="gt1"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="QCH03167.1"
                     /translation="MKVLFLTRDLSMLGGVERVIEALTTSLSKYHDCGVLTLVNAPMH
                     ESYNINSIQCLGLESPTNKFAQLKYHIKTFLSLSSIIKNNGCEYIVANSPSLSCLTIL
                     ASLFNKGKVKVICYEHNTFLFPNIIWRTLRRILFSHAHATIALTKDTYIDYIGINANP
                     IQVYNPITIKPGDRSRCDGFNVLALGRLTKQKGFDMLIDAWSIVASRNKKLQLHIVGD
                     GDERDSLEKKIADLSLQESITIHKADSNVQAYYSTSDLFVMTSRYEGFPLVLAEAMAH
                     GLPVVSFDCKSGPSDIINNKNGCLIKSGDIVALANKILTIFDDRDYLKRLSDGAMFTS
                     RDFDIDEISKKWNQEILT"
     gene            8577..9755
                     /gene="wzy"
     CDS             8577..9755
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="QCH03168.1"
                     /translation="MESRDTYMKAHSYCSFDFIFVSVALFIVTLYSSSSFSFMGCSLI
                     GVMLLILWAGLRIIKVGVKIDAVVIPLVVFSFFLMFLLLCRYSVEPGAYYSFDSDLKS
                     HFFTFSYIYIAIFLYAYFKVDYNAFYKVIFFIILCHVIFFVIQELLYYFVDYTLDFST
                     WLGGEGNRTGKSGADIFRGTGFFDEPSIYCGFLISLLSLSQLVKKTPAWLDSVVGGTV
                     IISFSFAGYIMLLLFVLNKYIKINFRGLLVICSVFLIFLYLYLDNEFIAGRLSKLFSG
                     DDSSTIYKSLYISYWLSQPEYFYVGTGISNDIHRPDFFDASYDLTFFGSIITTYGLVL
                     SLIVLLVFVFYFRRCRLRSINYFLIPLIKITVLHTPLFVFMFLSLCILNTQRRRCGTH
                     "
     gene            9742..10962
                     /gene="wzx"
     CDS             9742..10962
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="QCH03169.1"
                     /translation="MEHIRINIVILIFERTIQLLGALFIASLISRGLDTNEFGNWQLT
                     LSYLAIFSAFSIFCASEVIVPRFVRHQDSSVIGRIVFNVFVARLVTTVLCYLIIVGFV
                     FYIDSSKYNFVFAVLCFSVVINESISSFGAFLTAQKKSYYLSLARVLSFSIKVSLIYS
                     LYFFSNITPFKLAIVWITEASILGCVIFILYKKTINKVIFPVNVKYIRLLLSRSMIFG
                     VGLGFFILFRKLDKLFVSYYFTGFDLGVYAASSQLLEVVYQLIIVMAAVLAPIIIYDE
                     KALKLIVENKYKIIFWYSILSIMACYLVYLSFPFIYDMLFPVSYHEGKKYFLIVMWFF
                     PLFLVDNLVVLYIIQKLSLSFYVKKWVLTCVFFILSFVLLVKSMGVFATPISIILSIL
                     FTFLITLFYLRKVK"
     gene            11451..11570
                     /gene="orf11"
     CDS             11451..11570
                     /gene="orf11"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QCH03170.1"
                     /translation="MIIENVPAIDCINLKEELHAEVRKIKFIYAGNLQKKYEG"
     gene            11657..12058
                     /gene="orf12"
     CDS             11657..12058
                     /gene="gt2"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="QCH03171.1"
                     /translation="MDKKYNNINFLGRLSYVEMMSLAMSADVIIGMYYKSCPNHLYAS
                     PNKYYEHLALGKPLLTTIDTPPGVKVEINDTGWAIGEDAKDIRDFLSTINHLDIERKS
                     KNARAVWERKYNNYYQSIVDNYIKNVIGGKK"
     gene            12055..13362
                     /gene="purD"
     CDS             12055..13362
                     /gene="purD"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="QCH03172.1"
                     /translation="MNVLSLDPMYSPLHQMIMDRIADGNRYAILSSLGLSVYFNKTKI
                     IYFNKEVVKYKDNAVYIPSDILNLVLSDENHYSMFLKKIKKRDLNQDEIVYFSYFYIF
                     LENFIITNQIDLILLHNDLRWHHSLAIHLCKKHNLKYLVTEQGLFRPHTTVVDNVGVN
                     ALSNVKERFLYHVGQAECKSEVNYAITSSHDSYTSYYNFLRYLFLSKLGVVFGTESEY
                     VHKRHGVLDYLKRFYKIKIYPKITMLNNKKESPSTREKDVKRLIFVPLQLELDTQILV
                     HSDFINNQEIIDAITTSFINAKLESKYQLVFKLHPNDTNQYNFMAGVNVTDERITDTF
                     LEKVSLVIAINSSAILSILSTTIPIITLGRSIYNIEGVSEYSTISNLSDKINDVIKNG
                     CSLHNREKYLKYLKDKYSFNGAGYCYPDSEIERIVDELIMSEE"
     gene            complement(13898..14197)
                     /gene="tnp"
     CDS             complement(13898..14197)
                     /gene="tnp"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transposase"
                     /protein_id="QCH03173.1"
                     /translation="MKARIGIDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSAD
                     AGYQSTPQREELDEVDVDWRIAERSGKVKTLKQHPRKNKTAINIEYMKTSIRAQS"
     gene            complement(14214..14513)
                     /gene="insH"
     CDS             complement(14214..14513)
                     /gene="insH"
                     /note="for insertion sequence element IS5Y"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase InsH"
                     /protein_id="QCH03174.1"
                     /translation="MTAVIEPFYPKAGNGRQPYPLETMLRIHCMQHWYNLSDGAMEDA
                     LYEIASMRLSARLSQDSALPDHTTIMNFHHPLEQHQLPRQLFKTINRWLAEADVI"
     gene            14517..14651
                     /gene="orf16"
     CDS             14517..14651
                     /gene="orf16"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QCH03175.1"
                     /translation="MPWQNLLDAGEENLFSGLTAFTAEFTVGEGELMTHDGPWKCGSG
                     "
     gene            14805..15506
                     /gene="aqpZ"
     CDS             14805..15506
                     /gene="aqpZ"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="QCH03176.1"
                     /translation="MFKPFSAEFFGTFWLVLGGCGSALISAAFPQLGIGFLGVALAFG
                     LTVVTMAYAVGHISGAHFNPAVTLGLWAGGRFPAARVLPYIIAQVIGGIAAAAVLYGI
                     ASGKAGFDATTSGFAANGYGIHSPGGYALSACMLSEFVLSAFFVIVIHGATEKRAPAG
                     FAPLAIGLTLTIIHLVSIPVTNTSVNPARSIAAAVFQGTWALDQLWMFCLIPSLGGIA
                     GGLIYRALLARPAEA"
BASE COUNT     4328 a   2883 c   3418 g   4877 t
ORIGIN
        1 atgcgtttaa atccccgtca gcaggaagca gttgaatttg tcagcggacc ctgtctggtg
       61 ctggctggcg ccggctcggg aaaaacccgc gtaattacca acaaaattgc ccatctgatc
      121 cgcggctgcg gttatcaggc gcgccacatt gtggcggtga cctttaccaa caaggccgcg
      181 cgcgagatga aagagcgggt agcgcagacg ctcggtcgtg gtgaaaccaa aggcttgttg
      241 gtgtccacct tccactctct gggcttggaa atcttaaagc gcgaatacaa ggcgctgggg
      301 ctgaaagccg gtttttcgct gtttgacgat caagaccaaa tggcgctgct caaagagctg
      361 accgaagaca gcctcaaagg cgataaagac ctcctcagcc agctggtgca aaaaatctcc
      421 ggctggaaaa atgacatgat cttgcctgaa caggccaaag tgcgggccaa aggtgagcgc
      481 gatattctgt ttgccctgtg ttatgagcag tatgcacgcc agctacgtgc ctgcaatgtg
      541 ctggattttg acgatctgat tttgctgcca accttattat tgcgcaccca agaagaggtg
      601 cgagagcgtt ggcaaaatcg cattcgctat ttattggtgg atgagtatca agataccaac
      661 accagccagt acgagctggt gaaattgctg gtcggtgcgc gtgcccgttt caccgtggta
      721 ggggatgatg atcagtcgat ttactcatgg cgcggtgcgc gtccgcagaa tttaacctta
      781 ctgcgccaag attttccggc gttgaaagtg atcaagctag agcagaacta ccgctcctcg
      841 cagcgcatat taaaatcggc caacatcttg attgccaaca acccgcatga gtttgaaaag
      901 cagctgttct ccgagctcgg ccccggtgag atgctgcgtg tgctgcaggc aaacaacgaa
      961 gagcatgagg ccgagcgagt ggttggcgag atcatcgcgc atcgtttctt gaacaagacc
     1021 caatataaag attacgccat tttgtaccgc ggtaatcacc agtcgcgcac gctggaaaag
     1081 atgctgatgc aaaaccgcat tccgtacaaa atctcgggtg gcacttcctt ctttgcccgc
     1141 gccgaaatca aagacatcat ggcctacttg cgcctactga ttaaccctga tgatgacacc
     1201 gcgttcctgc gcgtagtgaa taccccacgg cgtgaaatcg gtccggtgac tttggaaaag
     1261 ctcggtacgt gggcgcacag tcgtggcaat agcctgtttg ccgctagctt tgatttggga
     1321 ttggagcagc acttgcaggg gcgtgggctg gaggctctgc ggcagtttac gcgttggatc
     1381 accgagattg ccgatcgtgc ccagcgtggc gacccggttg ccgcgctgcg tgatttggtg
     1441 catggcatcc attatgaaag ctggctctac gagacctccg ccagcccaaa agctgccgag
     1501 atgcgtatgc gcaacgtttc gaccctgttt ggctgggtga ccgagatgct ggaagggaaa
     1561 gatggcgagg agccgatgac catcaatcag gtggtgcaac gcctcacctt gcgcgacatg
     1621 ttggagcgcg gcgaagatga cgccgaagcc gatcaagtgc agttaatgac gctgcacgcc
     1681 tcgaaaggac tggagtttcc gcatgtcttc ttaattggga tggaagaggg gatcttgccg
     1741 caccagacca gtttggatga agacaatgtg gaagaagaac gccgtttggc atatgtcggg
     1801 atcactcgcg cccagaagac cttgaccttt accctatgta aagagcgccg tcagtacggt
     1861 gagttgctgc gcccagagcc aagtcgcttc ttgcacgaac tgccacaaga tgatttagag
     1921 tgggaagtgc gcaaggcgcc agtcagcgcc gaacagcgtc agcagcgtgg ccgccaaggg
     1981 gtggctggat tacgcgagat gttggcgcag gcgaaaaaac ggccagagtg atcttactgg
     2041 ccatgcgcgt agtgtttacg catatacgtg tctactcata taagcgacta cagatataag
     2101 tgcctaagta tttaggcact tataaatgcg taagacgccc gcaactggac gtacgcacta
     2161 tggcattgga atgccggaac gctggattat tccactcggt gacgggaagc caacaggcgg
     2221 tgatcttgca gtaattgcaa aaagcgtagc gcacgaactt ccccattgct ttcacgctcg
     2281 atatccacca aggcctgttg taaggcggta cacatcggct gactacagac tttaccctct
     2341 tccggcagtg caaccagcgg gttgtaacgt cgcgctaaat cgatcagcac ctcatccggc
     2401 actctcgcca acgtttcgcc aaaacgcacc aaccgcgcca cgccgcgcgt agcttgcact
     2461 cgcgtgacac tcagctgatc gggattaaag tgcacaaaca ggtaattggc aaacaacgcc
     2521 tcgctttcct cttgtgtctt accgcgacgc tgttttgtca ccgtcagcat cggacaaaaa
     2581 tagcggatat tttgcaactc cagatgctgc gccgcccgac tttgctgtcc gcgtttacaa
     2641 taaagaagat accaggcgtc catagatagt tttgtgatga aaataatgac tgcagtatgc
     2701 cacagagtgg atagccggtc agtctttggg ggtgatgtaa cagtcagttt aattaacaag
     2761 agggccgcac aaagtaccgt tcgcgcaaaa taccaccggt aaatcgtcag ttagagctag
     2821 agtcatttag cgcaaacaat catgaagaga catataaaag gaggcacaga ggcctccttt
     2881 tatctatttg cgtggtattt ttctttatat gctgattagt agtatcgcgt agcttataac
     2941 ccgctggtca tgtattcgat cgcggctttg atttcatcat cactacaatc ggtacaggtt
     3001 ccgcgtgccg gcatctgatt taaaccggcg atggcatgtt tttgcagggt atcgatacct
     3061 tgcttcagac gtggcgccca ctgcgcggca tcacgaaatt ttggtgcgcc taaggctccg
     3121 ctgctatgac aggcgataca agcgccgtta tacacctgct caccggtacg tggcccgctc
     3181 ggtttagcgg tagccgcagc atcagctaga tacaccccgc cgaccggttt gatgcgttcg
     3241 ctaattgcat catccgacat atccgctgcg gcagcccaac aggatacgcc gagcagcagg
     3301 gaggtcagca gagttaacgc cgcctggcga ggcttagcgc agatagactt ggcacgcaca
     3361 aagacagtgt tcacagattt tctccctaaa taaccagcgg catcctttta tcctgctttc
     3421 attcttaatc ggtctgagca gggctgtgaa ccgcagcggt tgaatattga ccgctcggtt
     3481 gtgttgccag attataagct aatcgtaaat aaattgaagc aattgcgcgg ggttcttttt
     3541 tgcaaggccg ctagcgtgcg acagcttgta caccccagcc tcacagaatt tacaaacata
     3601 ctgttatctt tttgacggta gatttggcct tacacaggca ttgctttata atctgcactc
     3661 caaattctgc gggctatccg ccggtttgta acagggaagt gggaggtgcg gtatatgcct
     3721 cttcacatgg agtgttctcg atcatgccct ctatcccaga tatcactttt atcgtcattg
     3781 gcgatatcct gttttgcttt tgcacagcgc tttgggagct gaaactcaag ggcggtagcg
     3841 tgcttttttc cagatttatt cttcattttt atttttaacc cattgataaa taatggattg
     3901 atttcatgtc aaaagcatct gaaccacaac agactcctta tcttatccca caaggggcct
     3961 atccaaccta tatgccaaaa gcagaggatg aaattgatct gttcgagctt ttaggcactt
     4021 tgtggaagaa aaagtgggtg attttatttg tcacattgct gactacagga ttagcggcag
     4081 tgtatgcctt tactgcaaaa gagcagtgga cagcaaaaac ttatattcag gccccacgta
     4141 ttgctgaact agggagttat cttaaatttc gtcaagcgta tgcacgtatt cttaatcaat
     4201 cattagatac aggtgcatta gcgagaggtt tattttcaga cttaatctta ttagccgaat
     4261 ctccagatac gaaagcctca tttttatcgc atactgaata ttataagaaa gagacagcag
     4321 atctctcatc agagacagag aagaaaatat ggttagataa ccaaattaac aagtctatag
     4381 tcatatctcc tcctgatgag aaaaaaggta gcaattacta cacagtagaa ttctctgcag
     4441 attcagccaa gaacgcatat caattattac aagcatacct taaagatgtt aatggtcagg
     4501 cggtgacgct aaacctttca gagttcgatc aaaatatcaa tgcactattg gtcaacttaa
     4561 aaaaagaagt gcaggatatt gatttcaaga agaaagcaga gaagttagat caaatagcat
     4621 ttattcaacg agatttaact acagcagaac aagcagggat tacagattat cgagctaata
     4681 aaaatggctt cgacactgca cagaatagct ataagttctt gttgggagaa aaactattat
     4741 cggctgaatt aaaatctgtg aaagatacac ccattattta tccatatcga tactatgaaa
     4801 ttaagcgtca agtagaagag ctcgaaggaa tgttacgtga taatattcaa gctcaagctt
     4861 tccgttatca gatggcacca atagaaccat taaagaaaga taaaccgaaa aaagcattgc
     4921 ttttggtgct tggtgcattg ttgggaggga tgtttggtgt cgctggcgta ttggtacagt
     4981 ctgtaattat taatagaaaa agaataaata agattgcgca gtagcattta aaataatgtg
     5041 aaatatttag tgtatataga ggaattacaa aatgcttacc tcggcagcca ttgcatttgt
     5101 gacgacatgg ttagcgattt tggtattaag gccgacatct caacgcattg ggttggttga
     5161 tgtgccgaat gctagaaagc tccatgtagg tgctgtacct ctcgtcggtg gaattgcggt
     5221 cagttttggt gtgctgatgg caacgctctt tggcttgatt ccacaaaacc caatgatcag
     5281 tattttgcta ttgggtggcg tgggtatggt gattatgggg gcaatagatg atgcaaaaga
     5341 tatctctcca tggctgcgct tattttttca agcgctacta atagcgttag tatgttatta
     5401 tggaaataca acgcttcata actttggtaa tctttttaat tttggtaata taaaaatccc
     5461 gacggctata gcatggcctg taaccatatt ggctgtttgt gctgcaatca atgcctataa
     5521 tatgatggat ggtattgatg gattagcagg ctgtatggct gctatctcat tggctgcact
     5581 aggttatacg ctatattctg ttgaaccaga tctttctaga ttatgttggg ttatcgtaat
     5641 agcacttata ccctacttga tggtcaatct aacgattcca ccttttagcc gtaaaatttt
     5701 cctcggtgat gctggctcta tgtttttggg ctttattatc ggggttttag tgctttatgg
     5761 atcacagcaa tcaaatggcc ataatgcatt taatcctatt acagctttgt ggtttattgg
     5821 ccttcctctc atggatatgc tcggtattat ggttcgccga atacgtaaag gccagtcacc
     5881 tttgcgcgca gatcgtaatc atctacatca tattttactt catgctggat tatcaccaaa
     5941 acaatcgcta ttactgatgg ttttaattaa tgttcttata gttacttgtg gcattttatt
     6001 ggagtcaagt ggtgctccgc aatggctgat ggtgactctg tatttggtat tgtttatgac
     6061 gtattcattg tgcttagctt atgcatgcaa actagtaagg gtaaatgata tctaatgtac
     6121 aagaatagat gttttcatga atcgtcaaga ttatccatct taattacatt tttatgaatg
     6181 tttaataatt aagctctttg ctattggaag aggtgctgct tttatcagtt cagctgttgt
     6241 gaatcatatt atccgatatg ctcaatgatt ttcactaaaa atatatgagt gcacttgtgt
     6301 aagtggtttt gaatatttag ataatatcta ataatttgag tgttgcattt tgtgagaaga
     6361 actgtgtttc tatcaccaag ataagttaat aaatgtgttg gtcaatatga aaaagaagaa
     6421 agttctgaca gtgtttggta cacgcccaga agcaatcaaa atggcgcctt tagttcatgc
     6481 tcttgccaaa gatgagcgtt ttgaagcaag ggtatgcgtc actgcacagc atcgtgagat
     6541 gctcgatcaa gttttagatt tgtttgagat taaaccagat tatgatttta atctgatgaa
     6601 agtaggtcaa acactcaatg aagtgaccgc aaaaatcctg ttagaattaa aaccggtatt
     6661 gcaagaattt aaacctgatg tggtattagt acatggcgat acagctacaa catttgctgc
     6721 tagcctagca gcgtattatg agcaaattgc tgtaggacat gtagaggcag gattacgcac
     6781 aggtaacatt tattctcctt ggcctgaaga agcaaatcgc aaattgacag gtgctctcac
     6841 ccgttatcat tttgcgccaa cagaaaccgc aaggcagaat ttgttacaag aaaattacgc
     6901 accagaaaat atctatgtca caggtaatac ggttattgat gcgttattga tggttaaggc
     6961 taaaattgat gctaaccctg ttctaaaaac aattctggca actcaattcc cttatcttga
     7021 tgaaaagaga aaattgattt tagtaactgg tcatcgtcgt gaaagttttg gtgacggatt
     7081 taaacgtatt tgtgaagcgt tatctttaat tgcaaaaaaa tatccagatg ttcagattgt
     7141 gtatcctgtt cacttaaatc ctaatgtgca aaaacctgta aatcgcttac taagtggctt
     7201 tgataatgtc tatttgatag agccacaaga gtatttacct tttgtatatt taatgaatcg
     7261 gtcatatctg attctgaccg attctggagg gattcaggaa gaagcgccat cactggggaa
     7321 accagttttg gtcatgcgag aaacgactga gcgtcccgag gctgtagatg ctggcactgt
     7381 tactctagta ggtacctgtg tgaaaacaat ttgctataaa gcgttttggc tattagaaaa
     7441 tgaatatgtt tattcaaaaa tgagcaagtc acacaatcca tatggtgatg ggaatgcggt
     7501 aattaagatt actgatgcca tatatagcaa taggtaattt atatgaaagt gttattttta
     7561 actcgagatt tatctatgct cggtggtgtt gaaagagtaa tagaagcatt aactactagt
     7621 ctgtctaaat atcatgattg tggtgttctg actttagtta atgcgccaat gcatgaaagt
     7681 tataatatca attcaattca atgtttaggt cttgagtcgc caacaaataa gtttgctcag
     7741 ttaaagtatc atattaaaac atttctatca ctttcctcta taattaaaaa taatggttgt
     7801 gaatacatag tggcaaactc tccttcattg agttgtttga caatattagc atcgctgttt
     7861 aataagggga aagtgaaagt catatgctat gagcataaca cgtttctatt tccaaatata
     7921 atttggagaa cattgcggag aatcttgttc tctcatgcgc atgcaactat tgctctaaca
     7981 aaagatactt atatcgatta cattggaatt aatgcaaatc cgattcaagt atataacccc
     8041 ataacaatta aaccaggaga tagatctaga tgtgatggct ttaatgttct tgcattaggt
     8101 cgtttaacta aacagaaagg ctttgatatg ttgattgatg cctggtctat tgttgcatct
     8161 cgaaataaaa aacttcagct tcatattgtt ggggatggag atgagagaga ttcattagag
     8221 aagaaaatag ccgatttgag tttacaagag agcataacaa tccacaaagc tgattcaaat
     8281 gtgcaagcct actatagtac ttcagacctc tttgttatga catcaagata tgaaggattt
     8341 ccattggtgt tggctgaagc aatggctcat gggttacctg tcgttagttt tgattgcaaa
     8401 tcaggaccat cagatattat taataataag aatggctgcc tcataaaaag tggagatata
     8461 gtggctcttg caaacaaaat acttactata tttgatgata gagattattt gaaacggtta
     8521 tctgatgggg caatgtttac atcaagagat tttgacatcg atgaaatatc aaaaaaatgg
     8581 aatcaagaga tacttacatg aaggctcact cttattgctc atttgatttt atctttgttt
     8641 ctgtagcact gttcattgta acattatact catcatcatc attctcattc atggggtgct
     8701 cgttaattgg ggtgatgctt cttatcttat gggcggggtt gagaattata aaggttggtg
     8761 tgaagattga tgctgtcgtt ataccattag ttgttttctc atttttttta atgtttttac
     8821 tactatgtag atatagtgtt gaacctggtg cttactatag ctttgactca gaccttaaga
     8881 gccatttttt tacattttca tatatttata tagccatatt tttatatgcg tattttaagg
     8941 tagattacaa tgcattctat aaagtgattt tttttataat tttatgtcat gtgatttttt
     9001 ttgttattca agagctttta tattattttg tggattatac attggatttc tcgacttggc
     9061 ttggtgggga ggggaataga acaggaaagt ctggagctga tatatttagg ggaacaggat
     9121 tttttgatga gccatctatt tattgtggtt ttttaatttc tctattatct ctgtctcagt
     9181 tggttaaaaa aacgccggcg tggttggatt cagtagtggg ggggaccgtc attattagtt
     9241 tttcttttgc cggttatata atgttactat tatttgtttt aaataaatat atcaaaatca
     9301 actttcgcgg attgttggtt atatgctctg tttttttgat ttttttgtat ttatatttag
     9361 ataatgagtt tatagctgga cgattaagta aattattttc aggtgatgac tcaagtacaa
     9421 tctacaagtc gctatatata tcttattggc tatcacaacc agaatacttt tatgttggga
     9481 ctggtatttc aaatgatata cacagacctg atttttttga tgcctcgtac gatttgactt
     9541 tttttggatc gattataacg acttatggat tggttctatc gttaatagtc ttgttggtgt
     9601 ttgtgtttta ttttaggagg tgtagactta gaagtataaa ctatttttta attccgctta
     9661 taaaaattac tgtacttcat acgccactgt ttgtttttat gtttttgtct ctctgtatac
     9721 ttaacaccca aaggcggcga tgtggaacac attagaatta acattgttat tttgatattt
     9781 gaacgaacca tacagctatt gggagcttta tttattgctt ccctcatatc tagagggcta
     9841 gatactaatg aatttggtaa ttggcaatta actctatcat atctagccat attttccgca
     9901 ttctctatat tttgtgcttc agaagttatt gtaccaagat tcgttaggca tcaagatagt
     9961 agtgttatag gaagaattgt ttttaatgta tttgttgcta gattggtaac aactgtctta
    10021 tgttacctga tcattgtcgg gtttgttttt tatatcgact ccagtaaata taattttgtt
    10081 tttgcggttt tgtgtttttc tgtagtcata aatgagtcaa tatcatcatt tggggcattt
    10141 cttaccgcac aaaaaaaatc atattattta tcattggctc gggtgttatc attttctatt
    10201 aaagtctcat tgatatattc actttatttt ttttctaata taacaccatt caagctggct
    10261 attgtatgga ttactgaagc ttcaatatta ggttgtgtta tatttatttt gtataaaaaa
    10321 acaataaata aagttatatt ccccgtcaat gtaaaatata tacgtcttct tctgagtcga
    10381 tctatgattt ttggtgtcgg actcggtttt tttatattat ttagaaagct agataaattg
    10441 ttcgtatcct attactttac tggctttgat cttggggttt acgcagcttc atctcagtta
    10501 ttagaagttg tatatcagtt aatcatagtg atggcggctg ttttggcacc gatcattata
    10561 tatgatgaaa aagcacttaa attaatagtc gaaaataaat acaaaattat attttggtac
    10621 tcaatcctgt ctataatggc atgttatttg gtttatctat catttccatt tatttatgat
    10681 atgctttttc cagtttcata ccatgaaggg aaaaaatatt ttttgattgt aatgtggttt
    10741 ttccctttgt ttttggttga taacttggtt gtcctttata ttattcaaaa gctatcacta
    10801 tcattttatg taaaaaaatg ggtgttgact tgtgtatttt tcatcttatc ttttgttctt
    10861 ttagttaaaa gcatgggggt tttcgccacg cctatttcga taatattatc aatattattt
    10921 acttttttaa ttacattatt ctacttacga aaggttaaat aatgatatta tttataagat
    10981 cgtcaaacta ccatatcgat ggtcgattat ctagatatct taatatattt aaagggaagt
    11041 tagattctgg tgttttatac tgggatagga aaaatgagaa cgcgaaaaac gataacttca
    11101 gattttactt tccaagttca tatggtaatg gtctgcgatc aataattggt tttgtattat
    11161 ggaattttta cattctattc tttctgataa aaaatagaaa agctataact tgtgttcatg
    11221 caataaatct agatacgatt attcctgttc taatcgcaaa gccaattata aaagggaagg
    11281 ttgtatatga tatctatgat tgttatgcgg atagtcattc aataggcggg ctgatgaatt
    11341 attttgttaa gagggttgag agactttgtt gttctctatc tgatacaatc atcttgcctg
    11401 atgaaagcag aataaagcaa tcattattag aaaatagtga tttcgatatc atgattattg
    11461 aaaatgttcc tgctattgat tgtattaacc ttaaagaaga acttcatgcg gaagtaagaa
    11521 aaataaagtt tatttatgca ggtaacttgc aaaaaaaata cgagggatag aaagcttcat
    11581 cgaaagtatt gcactaattg atcaggggga actatatatt tgcggtgatg gtgagttaaa
    11641 ggattattgc ctagaaatgg ataaaaagta caataacatt aattttttag ggcggcttag
    11701 ttatgttgaa atgatgagtt tagcaatgag tgctgatgta ataattggca tgtattataa
    11761 atcctgccca aaccatttgt atgcatctcc caataaatat tatgaacatc ttgctttggg
    11821 taaacctctt ttaacaacaa ttgatactcc acctggggtt aaagttgaaa ttaatgatac
    11881 aggttgggct attggtgagg atgcgaaaga tattcgtgat tttttatcaa ctataaatca
    11941 tcttgatatt gagcgtaaat caaaaaatgc tagagctgta tgggaaagaa aatataataa
    12001 ctattatcaa tctattgttg ataactatat caagaatgtc ataggtggaa aaaaatgaat
    12061 gttttatcgt tagatcctat gtattctcct cttcaccaga tgattatgga tagaattgct
    12121 gatggtaata ggtatgcaat tctttcttca ttaggcttga gtgtatactt taataaaaca
    12181 aaaattattt attttaataa ggaggttgtg aaatacaaag ataatgctgt ttatattcca
    12241 tcagatatat tgaatcttgt gctgagtgat gagaatcatt attcaatgtt tttaaaaaaa
    12301 ataaaaaaaa gagaccttaa ccaagatgag atagtgtatt tttcatattt ttatattttt
    12361 cttgaaaatt tcattataac aaatcaaata gatcttattc tgctacataa tgatcttaga
    12421 tggcatcatt cattggctat ccatctgtgt aaaaaacaca atctgaagta tttagtcaca
    12481 gagcaaggat tatttagacc acatactacg gttgttgata atgttggtgt aaatgcctta
    12541 tcgaatgtaa aggaaaggtt tttatatcat gttggacagg cggaatgtaa aagtgaagta
    12601 aattatgcaa taacttcatc tcatgattca tatacttcat attataattt cttaaggtat
    12661 ttgtttttat cgaagttagg tgttgttttt ggtactgaat cagagtatgt gcataaaaga
    12721 catggtgtat tggattattt aaaacgtttt tataaaataa aaatatatcc aaaaataact
    12781 atgttaaata ataaaaaaga atcaccaagt acgcgtgaga aggatgtcaa gcgtttgata
    12841 tttgtaccac tccagcttga attagatacg cagatattgg tacatagtga ttttattaat
    12901 aatcaagaga ttatagatgc gattacaact tcgtttataa atgcaaagct agagtctaag
    12961 tatcaattag tatttaagtt acatcctaac gatactaatc agtataattt catggctgga
    13021 gtaaatgtaa cggatgagag gataacggat acatttttag aaaaagtatc tttggttatc
    13081 gctattaact caagtgctat tttgagcata ttatccacaa ccataccgat aatcacttta
    13141 ggtagatcaa tatataatat tgaaggcgtg tctgagtatt ccaccatctc taatttatct
    13201 gataaaatca atgatgtaat taaaaatggt tgctcgctac ataatcgtga gaaatatctg
    13261 aagtatctta aagataagta ttcgttcaat ggtgcgggat actgttatcc tgacagtgaa
    13321 attgagcgta tagtagatga gctaataatg tcagaggagt aattgtaatg ttatttatct
    13381 ccgattctgt gtgattaaat tagatgatct tgttgtttaa atgtttgaaa attttagata
    13441 aataactaat taattcggag ttgagtttat tgcaatatcc tcttttataa aagcaaatcg
    13501 ctctaccata agctctttta ctgctccagg agtggataat ggattgagaa ttttgcaaaa
    13561 tgcaaaaaat tagggggtaa gatattaact gatgtacaag ggaaggtgcg aataagcgga
    13621 tcatttcttc ccaagctgat tcgctgatta aaatttcgcg tagctgcggc gttttttccc
    13681 gcaagcacct tgaacaagcc tgtcttgacc atttttcgcc acttaaggcg ttattcccag
    13741 tttcgagtga gatccttccc gctggcgtat catctgatcc acccgaaaca ggttggccag
    13801 ggtgaataat atcgccagtt agttatcgtt tttcagcagc cccttgtatc tggctttcac
    13861 gaggcctaac tgccgcttaa tgatgcgaaa cgggtgatca actttgagcc cggatactgg
    13921 ttttcatgta ttcgatgttg atggccgttt tgttcttgcg cggatgctgt ttcaaggttt
    13981 tcaccttacc ggaacgctcg gcgatccgcc agtccacatc cacctcgtcc agctcttcgc
    14041 gctggggcgt gctttggtag ccagcatcgg cggagacaaa ttgctcttct ccatgcagca
    14101 gattacccag ttgattgagg tcatgctcgt tggctgcggt ggtaactagg ctgtgagtaa
    14161 ggccgctctt ggcatcgata ccaatgcggg ccttcatgcc aaagtgcctt gggtcatatg
    14221 acgtctgctt cagccagcca gcgattgatg gtcttgaaca attggcgagg cagttgatgc
    14281 tgctccagcg ggtggtggaa attcatgatg gtggtgtggt ccggcaaggc gctatcctgg
    14341 gataaccggg cagacagacg catggaggcg atttcgtaca gagcatcttc catcgcgcca
    14401 tcactgagat tgtaccaatg ctgcatgcag tgaatgcgca gcatggtttc cagcggatag
    14461 ggctgacgcc cattaccggc tttgggataa aacggctcaa tgacagcggt catatcttgc
    14521 cgtggcagaa tctgctagat gcgggagagg aaaatctctt ttcgggtctg acggcgttta
    14581 ctgctgaatt cactgtcggc gaaggtgagt tgatgactca tgatggccct tggaaatgcg
    14641 gctctggatg aacatcatga tttcatatca cagacttgtt cgcaccttcc ttccatgtaa
    14701 ccttccacac taatccttca cctctcttac aaatccccgc taggcggtac atcgtgaccg
    14761 cctttaccct gatgcctgct ctttaacaaa caggacatca gtgtatgttt aaacccttta
    14821 gcgccgaatt tttcggcact ttctggctgg ttctgggtgg ttgtggtagc gccttgatct
    14881 ctgctgcttt ccctcagtta ggtattggct ttttgggcgt ggcgttggct tttggtctga
    14941 cagtagtcac catggcttat gcggtcgggc acatctccgg agcgcatttt aaccccgcgg
    15001 tgaccttggg tctgtgggcc ggtggacgct tcccagcagc gcgcgtgtta ccttacatca
    15061 tcgctcaggt tatcggcggt attgccgctg cggcagtgct gtatggtatc gccagcggta
    15121 aggctgggtt tgatgcgaca accagcggtt ttgcggctaa tggttatggc atccattccc
    15181 ctggcggcta tgcgttaagc gcctgtatgc tgagcgagtt tgtcctcagt gcgttttttg
    15241 tcatcgtgat ccacggagcg acagaaaaac gcgctcctgc gggctttgcg cctctggcga
    15301 ttggtctgac gctgaccatc atccatctgg tgagcatccc tgtcaccaat acctcagtta
    15361 acccagcgcg cagtatcgcg gcggcggttt tccaaggtac ttgggcatta gatcagttgt
    15421 ggatgttttg cctgatcccg tcattaggcg gaattgccgg tggtctgatt taccgcgcat
    15481 tgttggcgcg tccggctgaa gcataa
//
