LOCUS       MK551185               14430 bp    DNA     linear   BCT 05-MAY-2019
DEFINITION  Plesiomonas shigelloides strain O32H37 putative O antigen gene
            cluster, complete sequence.
ACCESSION   MK551185
VERSION     MK551185.1
KEYWORDS    .
SOURCE      Plesiomonas shigelloides
  ORGANISM  Plesiomonas shigelloides
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacterales incertae sedis; Plesiomonas.
REFERENCE   1  (bases 1 to 14430)
  AUTHORS   Xi,D., Wang,X., Ning,K., Liu,Q., Jing,F., Guo,X. and Cao,B.
  TITLE     O-Antigen Gene Clusters of Plesiomonas shigelloides Serogroups and
            Its Application in Development of a Molecular Serotyping Scheme
  JOURNAL   Front Microbiol 10 (2019) In press
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14430)
  AUTHORS   Xi,D., Wang,X., Ning,K., Liu,Q., Jing,F., Guo,X. and Cao,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2019) TEDA, Nankai University, Hongda Street 23,
            Tianjin 300457, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: VelvetOptimiser v. v2.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14430
                     /organism="Plesiomonas shigelloides"
                     /mol_type="genomic DNA"
                     /strain="O32H37"
                     /db_xref="taxon:703"
     gene            1..2031
                     /gene="rep"
     CDS             1..2031
                     /gene="rep"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="QCH03223.1"
                     /translation="MRLNPRQQEAVEFVSGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIVAVTFTNKAAREMKERVAQTLGRGETKGLLVSTFHSLGLEILKREYKALGLK
                     AGFSLFDDQDQMALLKELTEDSLKGDKDLLSQLVQKISGWKNDMILPEQAKVRAKGER
                     DILFALCYEQYARQLRACNVLDFDDLILLPTLLLRTQEEVRERWQNRIRYLLVDEYQD
                     TNTSQYELVKLLVGARARFTVVGDDDQSIYSWRGARPQNLTLLRQDFPALKVIKLEQN
                     YRSSQRILKSANILIANNPHEFEKQLFSELGPGEMLRVLQANNEEHEAERVVGEIIAH
                     RFLNKTQYKDYAILYRGNHQSRTLEKMLMQNRIPYKISGGTSFFARAEIKDIMAYLRL
                     LINPDDDTAFLRVVNTPRREIGPVTLEKLGTWAHSRGNSLFAASFDLGLEQHLQGRGL
                     EALRQFTRWITEIADRAQRGDPVAALRDLVHGIHYESWLYETSASPKAAEMRMRNVST
                     LFGWVTEMLEGKDGEEPMTINQVVQRLTLRDMLERGEDDAEADQVQLMTLHASKGLEF
                     PHVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQKTLTFTLCKERRQYGELLR
                     PEPSRFLHELPQDDLEWEVRKAPVSAEQRQQRGRQGVAGLREMLAQAKKRPE"
     misc_feature    1..14430
                     /note="putative O antigen gene cluster"
     gene            complement(2187..2597)
                     /gene="rfaH"
     CDS             complement(2187..2597)
                     /gene="rfaH"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="QCH03224.1"
                     /translation="MQDIRYFCPMLTVKKQRRGQWQEEAEALFANYLFVHFNPDQLSV
                     TRVQATRGVARLVRFGETLARVPDEVLIDLARRYNPLVALPEEGKVCSQPMCTALQQA
                     LADIERESSGEVRALRFLQLLQDHRLLASRHQVE"
     gene            complement(2949..3275)
                     /gene="c5"
     CDS             complement(2949..3275)
                     /gene="c5"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5"
                     /protein_id="QCH03225.1"
                     /translation="MSDDAINERIKPVGGVYLADAAATAKPSGPRTGEQVYNGACIAC
                     HGSGALGAPKFRDAAQWAPRLKQGIDTLQKHAIAGLNQMPARGTCTDCSDDEIKAAIE
                     YMISGL"
     gene            3939..5015
                     /gene="wzz"
     CDS             3939..5015
                     /gene="wzz"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz"
                     /protein_id="QCH03226.1"
                     /translation="MSKATEPQQTPYLLPQGTYPVYMPKADDEIDLFELMATLWKKKG
                     IIVLVTFLTTALAAGYAFTAKEQWTSEAIVTLPSAAAVSNLYSGYRLLDPNYSVDSAQ
                     NMLFKRFLDDSASYDNLARYLADTAYFKELVSGKTEKAKMQILDDWIKQITIQLVDKN
                     VADKVRVALPADNALQAQQLLRQYLVQSSQQAVQQSYADLEQQIKAQKASLADAMMAS
                     RTVAEVKRQTEIADIESSLLIAEKAGITKPELSAGMELKQNNLFLLGSGPLSAMLQGI
                     KKQPLVLSDKYYEQQTNLYSLEKYQPNNEQVSAFSYLKAASDPVTKDKPKKALIMVLG
                     ALLGGMLGVAGVLIQSVISSRKNT"
     gene            5089..6042
                     /gene="gne"
     CDS             5089..6042
                     /gene="gne"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="QCH03227.1"
                     /translation="MRVAIIGGSGFIGSVLSKALANKKIAFSILDKNKSSIFPEYWTY
                     CDVTKLDTLESSLREHDVIINLAAEHKDNVSPSDLYYQVNVDGARNVCCAAEKFGINK
                     VIFTSSVAVYGFVDQDTDETGEFHPFNHYGKSKLEAEQVYDQWQAASPTDRVLTTIRP
                     TVVFGEGNRGNVYNLFRQIASGKFLMIGKGNNPKSMAYVENVVAFLTHCLNLSTGRKI
                     FNYVDKPDFDMLSLANLVNSSLGKKTNSIRIPYLVGLSAGYCFDVLAKISGKEFPVSS
                     IRVRKFCARTQFSSLNIIETGFIAPVSLHDAVIKTVRHEFS"
     gene            6074..6973
                     /gene="exo"
     CDS             6074..6973
                     /gene="exo"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="QCH03228.1"
                     /translation="MNDSVVYIYCLEQDKERINKAIKNYPVNICFNCFDFDSYKNKTL
                     LHYKSGLLDERITELLIHATKKGAHVEPLVSYLDARFGHTEVELLHSGYFLHMKAFSI
                     LSRRSNRMFKRILDLCFVALLSVIAIPIGLITAIAIKKESPGPVFYRQKRVGQYNKEF
                     EVIKFRSMRADAEKNGAQWASKNDSRVTRVGNFIRKTRIDELPQLINVLKGEMSMVGP
                     RPEREVFIKELETVIPYYRFRHAVKPGITGLAQVKYPYGASIEDAVWKHKYDIYYIKH
                     QSFLMDVKILMLTIKTVLCGMGR"
     gene            6970..7986
                     /gene="gt"
     CDS             6970..7986
                     /gene="gt1"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="QCH03229.1"
                     /translation="MKTVIFIITKSEIGGAQSWVYEQAKIVSSDCNVILVTSEDGWLT
                     RQSVFSEVYLLPGIAKLIKIPTLFKLINIIKDHSVFSVVSSSANAGVYARLARLFIKF
                     KCVYVSHGWSCLYNGGSFSNLFCKVEKYLSILTDTVWCVSKSDRDKAIGIIGINENKI
                     ITSLNSITPLPPKLDGEFMGRVLFVGRLTHPKRPELIADVIANNKRYSLDIIGDGDLY
                     PELYKRYGECENISFLGEISDFRDFYKYDVFVLTSDSEGLPMSALEAGSAGLPMILSN
                     VGGCFEVIGGENGILVENNISDIRTSLNLIADNYNYYLDNARNYKSEFDISSRAAEYK
                     KIIM"
     gene            8127..9365
                     /gene="wzx"
     CDS             8127..9365
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="QCH03230.1"
                     /translation="MLVEKILTHYKKNNDIVNNILFLCVEKVFVFIMVFYVEAMISRA
                     LGIENYGKWLYAVNLILVISSLTLVAGSEVVVPALARVKNVQLQWDILTGAFIIRMAL
                     SVVTFIGIYVYICFFIHDESLKLMLKPLAFVLLLIEPFGVITNYYQSKIRSWPIVLAR
                     LFALFIRCVVVSFAVWVGHDVAIYFCRSIEALVLALILSVLILRCGYKCVVSPRVINK
                     IFIRGLKLWAPLILMLIYMRVDRFFVENYLGYKELASYGVAAQVMEQLMLLVSIVIMS
                     IGPKLIFKKLSIDKRKVVLLISLITIPIQIFAAFSLPFFIGRVFGSDYDNSAIMAIKM
                     LPALFFYAIDMVYMQYIYKNRLYHVVFIKWALMLLICMASYYFWLSVMGRKDIAPVFV
                     FNYICMLVVTILVSRIRKVN"
     gene            9375..10199
                     /gene="gt"
     CDS             9375..10199
                     /gene="gt2"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="QCH03231.1"
                     /translation="MSDNMLFTIFTPAYNRAHTLKRCYDSIVMQSFISLEWIVIDDGS
                     IDNTRDVVKGFIDEGKINIRYFYQDNAGKQAAWNKALQEAKGEFFVGLDSDDALVPNS
                     LIKMNGNLIKIRNSELVLGVRAVAISEKKKCADSDFKVLGNGVASWFDEFASRIFGER
                     IDILKTSEIKKFLYPVGPGIKFIPEVWFYAKTASSGYLFLYETEPVRLFFDEHNHMRL
                     SRSNLRAHAKGHLISRAEMLKVIPISAWARNPIALIKTIVRTLQCIIFCILDSKKK"
     gene            10160..11284
                     /gene="gt"
     CDS             10160..11284
                     /gene="gt3"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="QCH03232.1"
                     /translation="MHNILYFGFKEKMKICFFIGGLNSSGGTERVSSVIASELQQRGY
                     QIHMLNLQCGDKPFFELADGIQVNQLFTSVGRGMLRLPLVIVKLRRYLKQHKIDILID
                     VESMLALYALPAVIGLNVRHICWEHFNYNVDLGKASRRLARKLAARFADDVVTLTERD
                     KQLWLANTTCKARITTISNPVTISLPSEINLQKEKLFLAVGRLTYQKGFDLLLQAWAQ
                     VAPLYPDWRLRIVGDGEDKAMLEQLCCELNIEASTELVPKTKNIAVYYQEAAYFVMSS
                     RFEGFGLVLVEAQAYGLPIISFDCDVGPSDIIINDSTGWLCRQNDVFSMAHKIRNAIS
                     IYNEDNDRYYSMCSESIVNSRRFSIDVILNEWIDLIERIS"
     gene            11315..12463
                     /gene="wzy"
     CDS             11315..12463
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="QCH03233.1"
                     /translation="MTSARLKLVERDNFAITLLVILSFCIDLSSPIITYGVQFVLVFV
                     LAFYTIFARESPSIPRGNFVLFFILIITVTVFTLYQHTYLEHQELLFLQYFRVLLWMA
                     CGFLFYAYLLSQQSATIARALMVTILITGGALYIQYLVYYAFGYVIDYSLLLGGSASR
                     IFFLTFRPSGFTAEPAVYSGIMIGLLSVYYMFVRRLNMIVIFGLISVVLTSSIAGLMM
                     SVLYVLVVFFSRPSLGRIVISIAALFIIIGLFFPVIQERYVTTVSGSDASNNTKFETV
                     INMYEHSNLLLSGYGFVGKSDSAPSFYEGLYDMTFFGATIITFGLPLGLCICFFVVYI
                     ILTQPADGKILILVSLIKLSSPMCMFFNLFVALVFVVRYKARLNILSV"
     gene            12466..13554
                     /gene="gt"
     CDS             12466..13554
                     /gene="gt4"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="QCH03234.1"
                     /translation="MHILLVITGLGMGGAERQVCDLADQFSKSGHSVLLISLTGEVIN
                     RPQSNNVEVVNIEMKKTPVGFTKAYCRVRQLIKAFAPDVVHSHMVHANLFARFLRLTV
                     SMRKLICTAHNTNEGGWVRMLAYRISDSLCDISTNVSQEAVDAFISHKAVPANRMLPM
                     HNGIDTDRFLFDSSMRKKLRKKLNLTDYDSLILAVGRLNAQKDYPNLLTAFSLIVSVN
                     PEIQLAVIGVGEEKSVLTSMVDDLGLNKHVHFLGLQHNVNEWLSAADVFVLSSAWEGF
                     GLVVAEAMACKRVVVATDCGGVKEVLGDCGFLVPPQNSQALADGLSAALSLTSDEARV
                     KGELARQRVIEHYSLQEQAKRWLELYQR"
     gene            13729..14430
                     /gene="aqpZ"
     CDS             13729..14430
                     /gene="aqpZ"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="QCH03235.1"
                     /translation="MFKPFSAEFFGTFWLVLGGCGSALISAAFPQLGIGFLGVALAFG
                     LTVVTMAYAVGHISGAHFNPAVTLGLWAGGRFPAARVLPYIIAQVIGGIAAAAVLYGI
                     ASGKAGFDATTSGFAANGYGIHSPGGYALSACMLSEFVLSAFFVIVIHGATEKRAPAG
                     FAPLAIGLTLTIIHLVSIPVTNTSVNPARSIAAAVFQGTWALDQLWMFCLIPSLGGIA
                     GGLIYRALLARPAEA"
BASE COUNT     3897 a   2669 c   3417 g   4447 t
ORIGIN
        1 atgcgtttaa atccccgtca gcaggaagca gttgaatttg tcagcggacc ctgtctggtg
       61 ctggctggcg ccggctcggg aaaaacccgc gtaattacta acaaaattgc ccacctgatc
      121 cgcggctgcg gttatcaggc gcgccacatt gtggcggtga cctttaccaa caaggccgcg
      181 cgtgagatga aagagcgggt agcgcagacg ctcggtcgcg gtgaaaccaa aggcttgttg
      241 gtgtccacct tccactctct gggcttggaa atcttaaagc gcgaatacaa ggcgctgggg
      301 ctgaaagccg gtttttcgct gtttgacgat caagaccaaa tggcgctgct caaagagctg
      361 accgaagaca gcctcaaagg cgataaagac ctcctcagcc agctggtgca aaaaatctcc
      421 ggctggaaaa atgacatgat cttgcccgag caggcaaaag tgcgcgccaa aggtgagcgc
      481 gatatcttgt ttgccctgtg ttatgagcag tatgcacgcc agctacgcgc ctgcaatgtg
      541 ctggattttg atgatctgat tttgctgcca accttattat tgcgcaccca agaagaggtg
      601 cgagagcgtt ggcaaaatcg gattcgctat ttattggtgg atgagtatca agataccaac
      661 accagccagt acgagctggt gaaattgctg gtcggcgcgc gcgcccgttt caccgtggta
      721 ggggatgatg atcagtcgat ttattcatgg cgcggcgcgc gcccacagaa tttaaccttg
      781 ctgcgccagg attttccggc gttgaaagtg atcaaactgg agcagaacta ccgctcctcg
      841 cagcggatat taaaatcggc caacatcctg attgccaaca acccgcatga gtttgaaaaa
      901 cagctgttct cggagctcgg ccccggtgag atgctgcgtg tgcttcaggc aaacaacgaa
      961 gagcatgaag ctgagcgcgt ggtcggcgag atcatcgcac accgtttctt gaacaagacc
     1021 caatataaag attacgccat tttgtaccgc ggtaatcacc agtcgcgcac gctggaaaag
     1081 atgctgatgc aaaaccgcat tccgtacaag atctcgggtg gtacctcttt ctttgctcgc
     1141 gccgaaatca aagacatcat ggcctacttg cgcctgctga ttaacccgga tgacgacacc
     1201 gcgttcctgc gtgtagtgaa taccccacgg cgtgaaatcg gtccggtgac cttggaaaag
     1261 ctcgggacgt gggcgcacag tcgtggcaat agcctgtttg ccgctagctt tgacttggga
     1321 ttggagcagc atttgcaggg gcgtgggctg gaggctctgc ggcagtttac gcgttggatc
     1381 accgagattg ccgatcgcgc ccagcgtggc gatccggtcg ccgcgctgcg tgatttggtg
     1441 catggcatcc attatgaaag ctggctctac gagacctccg ccagcccgaa agccgctgag
     1501 atgcgcatgc gcaacgtctc taccctgttt ggctgggtga ccgagatgct ggaaggcaaa
     1561 gacggcgagg agccgatgac catcaatcag gtggtacagc gcctcacctt gcgcgacatg
     1621 ttggagcgcg gcgaagatga cgccgaagcc gatcaagtgc agttaatgac gctgcacgcc
     1681 tcgaaaggac tggagtttcc gcatgtcttc ttaattggga tggaagaggg gatcttgccg
     1741 caccagacca gtttggatga agacaatgtg gaagaagagc gccgcttggc atatgtcggg
     1801 atcacccgcg ctcagaagac cttgaccttt acgttatgta aagagcgccg ccagtacggt
     1861 gagttgttgc gcccagagcc gagccgcttc ttgcacgaac taccgcaaga tgatttagag
     1921 tgggaagtgc gcaaggcgcc agtcagcgcc gaacagcgtc agcagcgtgg ccgccaaggg
     1981 gtggccggat tacgggagat gttggcgcag gcgaaaaaac gcccagagtg atcttactgg
     2041 ccatgcgctt agtgtttacg catataagtg tctactcatg taagtgacta cagagataag
     2101 tatctaattc tttaggcgct tatctctgct gaaaacaccc acaacggtac gcacgttcta
     2161 taacattgga atgctgggag gctggattat tccacttggt gacgggaagc caacaggcgg
     2221 tgatcttgca gcaattgcaa aaagcgtagc gcacgaactt caccactgct ttcacgttcg
     2281 atatccgcta aggcctgttg taaggcggta cacatcggct gactacagac tttaccctct
     2341 tccggtagtg caaccagcgg gttgtaacgt cgcgctaaat cgatcagcac ctcatccggc
     2401 actctcgcca acgtttcgcc aaaacgcacc aaccgcgcta cgccgcgcgt agcttgcact
     2461 cgcgtgacac tcagctgatc gggattaaag tgcacaaaca ggtaattggc aaacagcgct
     2521 tcggcctctt cctgccattg acctcgacgc tgttttttta ccgtcagcat cggacaaaaa
     2581 tagcggatat cttgcaactc cagatgttgc gccgcccgac tttgctgtcc gcgtttacaa
     2641 taaagaagat accaggcgtc catagatagt tttgtgatga aaataatcac tgcagtatgc
     2701 cacagagtgg atagccggtc agtctttggg tgtgatgtaa cagtcagttt aattaatgag
     2761 agggctgcac aaagtactgg tcgggtaaaa taccgccgct aaatcgtcag ttagagctag
     2821 ttaagtcaga gtcatttagt gcaaacgatc atgatagaca tataaaggag gcgccgaggc
     2881 ctcctttatc tatttaccct atctgcgcgg tattttcttt tatatgctga ttagtagtat
     2941 cgcgtagctt ataacccgct aatcatgtat tcgatcgcag ctttgatttc atcatcacta
     3001 caatcggtac aggttccgcg tgcaggcatc tgatttaaac cggcgatagc atgtttttgc
     3061 agggtatcga taccttgctt cagacgtggc gcccactgcg cggcatcacg aaacttaggt
     3121 gcgcctaagg ctccgctgcc atgacaggcg atacaggcgc cgttatacac ctgctcaccg
     3181 gtacgcggcc cgctcggttt agcggtggcc gcagcatcag ccagatacac tccgccgacc
     3241 ggcttgatgc gttcgttaat cgcatcatct gacatatcgg ctgcggcagc ccagcatgat
     3301 acgcccagca gtagggaggt cagcagagtt aacgccgcct ggagaggctt agcgcagata
     3361 gacttggcac gcacaaagac agtgttcaca gattttctcc ctaaataacc agcggcatcc
     3421 ttttatcctg ctttcattct taatcggtct gagcagggct gtgaaccgaa gtgattgaat
     3481 attgaccact cggttgtgtt gccagattat aaggtaatcg taaataaatt gaagcaattg
     3541 cgcgggattc ttttttgcaa ggcggactgt gagcgacagc ttgtacagtc cagcctcaca
     3601 gaatttacaa acacactgtt atctttttga cggtagattt ggccttccat aggcattgct
     3661 ttataatctg cactccaaat tctgcgggct atccgccggt ttgcaacagg gaagtgggag
     3721 gcgtatcttg gcgtctcggc cggatcacag gttttcttcc ccctgatact cttcccgtat
     3781 tgcatgctgc cgcagcaatg ccttcatatt ttgcagcgct ttaggagctg aaacctaagg
     3841 gcggtagcgt atttgtttgc tttttatagg caagcaaaac ttttcctatt ttgctgctga
     3901 ttttatttaa ctcattgata gataatggac tgacatctat gtcaaaagca actgagccgc
     3961 aacagactcc ctacttactg ccgcagggca cgtaccctgt gtatatgccc aaagctgatg
     4021 atgaaattga cttgtttgag ttgatggcaa cgttgtggaa aaagaaaggg ataattgtac
     4081 tggtcacgtt tttgactacg gcattagcgg caggttatgc gtttaccgca aaagagcagt
     4141 ggacatcaga agcgattgtt actttaccga gtgccgcagc agtcagtaac ttgtacagcg
     4201 gttaccgctt gttagacccg aactatagcg tagattcagc acaaaacatg ctatttaagc
     4261 gttttttgga tgacagcgct tcttacgata atctggctcg ttatttggca gatacagctt
     4321 attttaaaga gctagtgtcc ggtaaaacag aaaaagcaaa gatgcaaata ctggatgatt
     4381 ggattaaaca gatcactatc cagctggtgg ataaaaatgt tgcagataag gtgagagtgg
     4441 ctttacctgc agataatgct cttcaagcac aacaattgtt acgtcaatac ttggtacaaa
     4501 gtagccagca ggcagtacaa caaagttatg ctgatttaga acaacaaatc aaagcccaaa
     4561 aagcaagcct agccgatgcc atgatggcat ccagaaccgt ggctgaagtg aagcgtcaga
     4621 cagaaattgc ggatatagaa tcttcgctct tgatcgcaga aaaagcggga attacaaaac
     4681 cagaattatc agcagggatg gagctaaaac aaaataattt attcttactc ggtagtggtc
     4741 cgttgtcagc tatgttacaa ggcattaaga aacagccgtt agtactaagc gataaatact
     4801 atgaacagca aacgaatttg tacagtctag aaaaatacca accgaataat gaacaagtct
     4861 cagcttttag ttatttgaaa gcagcatcag acccggtgac gaaagataaa cctaaaaagg
     4921 cactgattat ggtgttgggg gcgttgctgg gcggcatgct aggagtagcg ggtgtattga
     4981 ttcagtcagt gatttcatct cgaaaaaaca cttagcctta gggtgtgaca tcaatactat
     5041 caaggccttt attgagttgt gacaagttaa ggtataggga ataatctaat gagagttgca
     5101 atcatcggtg gttctggttt tattggtagt gtcttatcta aggcgctggc taataaaaaa
     5161 atagcatttt cgattttaga taaaaacaaa agttcaattt tccctgaata ttggacatat
     5221 tgtgatgtga caaagctcga tacgttagaa tctagtttgc gtgagcatga cgtcataatc
     5281 aaccttgcgg ctgaacataa agataacgtt agtccatcag atttatatta tcaagttaat
     5341 gttgatggag ccagaaatgt ttgttgtgcg gcagaaaagt ttggtataaa taaagtcatc
     5401 tttacttcat cagttgctgt ttatggtttt gttgatcaag atacggatga aacaggagag
     5461 tttcatccgt tcaatcatta tggaaaatcg aagttagaag ctgaacaggt atatgaccag
     5521 tggcaggccg catcaccaac tgatagagtt ttaacaacca ttcgacctac cgttgtgttt
     5581 ggcgaaggga accgaggtaa tgtatataat ttatttaggc aaattgcctc gggaaaattc
     5641 ttgatgatag gcaaaggaaa taatcctaag tctatggcgt atgttgagaa tgttgttgcc
     5701 tttctaactc attgcctgaa tctgtctaca gggcgaaaga tatttaatta tgtcgacaag
     5761 cctgattttg atatgctctc cttagctaac ttagtgaaca gtagcctagg aaaaaaaaca
     5821 aatagcatca ggattcctta cctagtgggg ctgtcagctg gttactgctt tgacgtgtta
     5881 gcaaagataa gtggtaaaga atttcctgtc agcagtattc gggtgcgaaa gttttgcgct
     5941 agaactcaat ttagttcttt gaatattatt gaaactgggt ttattgcacc agtatcattg
     6001 cacgatgctg ttattaaaac tgtcaggcat gaattttcct aaataaccta aggcaaagtg
     6061 gtttcagatt agtatgaatg attctgtagt gtatatttat tgtcttgagc aagataaaga
     6121 gagaattaat aaggcaataa aaaattatcc agtcaatatt tgttttaatt gttttgattt
     6181 tgacagttat aagaataaaa cattgctgca ctataaaagc ggtcttcttg atgagaggat
     6241 tactgagtta ctgattcatg ctaccaagaa aggggcgcat gtagagccat tggttagtta
     6301 tttagacgct cgctttggtc acactgaagt tgagttattg cacagtggtt attttctaca
     6361 catgaaagca ttttcgattc tatcgcgtcg cagtaatcga atgtttaaac gcatccttga
     6421 tctgtgtttt gttgcattgc taagtgtaat agcaatacct atagggctta ttaccgcgat
     6481 tgcgattaaa aaagagtctc caggcccagt attttaccga caaaaacgag ttggccagta
     6541 caataaagag tttgaagtta tcaaatttag atctatgagg gctgatgcag agaaaaatgg
     6601 tgcacagtgg gcaagcaaaa atgatagccg agttactcgt gttggtaact ttattcggaa
     6661 aacacgaatt gatgaattac cacaactcat taatgttcta aaaggtgaga tgtcgatggt
     6721 tggacctcgc cctgagcgag aggtctttat taaagagtta gagactgtta ttccttacta
     6781 tcgatttcga catgcagtaa aaccgggcat cacagggtta gctcaggtga aatatccata
     6841 tggtgcatca atagaggatg ccgtgtggaa gcataagtat gatatttatt atataaaaca
     6901 tcagagcttt cttatggatg tcaaaatctt gatgctgaca ataaaaacgg ttttatgtgg
     6961 gatggggcga tgaagacagt catttttatt attactaagt ctgagatcgg cggtgcccag
     7021 agttgggttt atgagcaagc aaaaattgtc tctagcgatt gtaacgttat tttagttaca
     7081 tctgaggatg gctggctaac aaggcaatca gtattttcgg aagtatatct actcccaggt
     7141 atagcaaagt taataaaaat accgacattg ttcaagttaa tcaatataat aaaagatcat
     7201 tctgtattct cagtagtttc aagttcggca aatgcagggg tttatgctag attagcaagg
     7261 ctatttatca agtttaagtg cgtctacgta tcgcacgggt ggtcgtgttt atataatggc
     7321 ggatcgttta gcaatctgtt ttgtaaagta gaaaaatatc tttcaatcct gacggatacc
     7381 gtttggtgtg tttcaaaaag tgatagagat aaagcgatag gaattatagg gattaatgaa
     7441 aataagataa taacatcatt aaattctatt actccattac caccaaagct agatggtgaa
     7501 tttatgggga gagtattatt tgtcggtcgt ttaactcatc caaaaagacc tgagttaatt
     7561 gctgatgtga tagctaataa taagcgttat agccttgata ttattggtga tggagatttg
     7621 tatccagagt tatataagcg ttatggagag tgtgagaata tttccttctt aggtgaaatt
     7681 tctgattttc gagacttcta taagtatgat gtatttgtgc taacatctga tagtgagggg
     7741 ttgccaatgt ctgccttaga agctggatct gcgggattac caatgatttt gagtaatgtg
     7801 ggtggctgct ttgaggttat tggcggggaa aatggtattt tggtagaaaa taacatttca
     7861 gatataagga catcattaaa tcttattgca gacaattaca attactacct ggataacgcc
     7921 agaaattata aatcagaatt tgatatatct tcgcgagcgg ctgagtataa aaaaataatt
     7981 atgtgattgc gtctatatgg ctatgcatgc tctgtatggc cgaaactatt taaactcatc
     8041 tatctttata gtcttttttc gatgaagtca tgtctatatc cgacatgtat ctattctttt
     8101 gacttaccct gcgtggttgt cttttcatgt tagtagaaaa aatcctcact cattacaaaa
     8161 aaaacaatga tatagttaat aatatacttt tcctttgtgt tgaaaaagtt tttgttttta
     8221 tcatggtttt ctatgttgaa gccatgatta gtagagcttt gggcatagag aattatggaa
     8281 agtggctata tgccgtaaat cttatactag tcatttcgtc gttgacattg gttgcaggtt
     8341 cagaggttgt agtgcccgcc ttagctaggg taaaaaatgt tcaattgcaa tgggatatat
     8401 tgactggtgc tttcattata agaatggcac tatcggtagt cacctttatt ggtatatatg
     8461 tttatatatg tttttttatt catgatgagt cattgaaatt aatgttaaaa ccattggctt
     8521 ttgtcttatt gctaatagag ccatttggtg tcattactaa ctactatcaa tcgaagatta
     8581 gatcttggcc aattgtttta gcaagactat tcgcgctatt tattagatgt gttgttgtga
     8641 gttttgctgt ttgggttggg catgatgttg ctatctattt ttgccgtagc attgaagctt
     8701 tggttttagc cttaatttta tcagtattga ttctacgttg tggttataaa tgtgttgtgt
     8761 caccaagggt gataaataaa atttttatta ggggactgaa attatgggct cccttgatat
     8821 tgatgctaat atatatgcga gttgatagat tctttgttga aaactatctt gggtataaag
     8881 aattggctag ctacggagtt gctgctcaag ttatggagca gttaatgttg ctagtgtcaa
     8941 tagtaataat gtcaattggt ccgaagctaa tttttaaaaa actatcaata gataagagaa
     9001 aggtagtatt attaatttca ctaattacta ttcctatcca aatttttgct gcattttctc
     9061 ttcctttttt tattggtcgg gtttttggct ccgattatga taactcggct attatggcaa
     9121 taaagatgct tccagcattg tttttctatg cgatagatat ggtctatatg caatatatat
     9181 ataaaaatag actttatcat gttgtattta ttaagtgggc gttgatgctg ctgatatgca
     9241 tggctagcta ttatttttgg ttgtctgtta tggggcggaa agatattgct cctgtttttg
     9301 tttttaatta tatatgcatg ctggttgtaa ctatattagt atcgcgtatt agaaaggtta
     9361 attagtggct gattatgagt gataatatgt tattcacaat atttacccct gcttataata
     9421 gagcgcacac cttaaaaagg tgctacgata gtatagttat gcaaagtttt atatctcttg
     9481 agtggattgt tattgatgat ggttcaatag ataatacaag agatgttgtt aaaggtttta
     9541 ttgatgaagg taagataaat ataaggtatt tttatcagga taatgccggt aagcaggctg
     9601 catggaataa agcccttcag gaggcaaagg gggagttttt tgttggtcta gattctgatg
     9661 atgcattagt gcctaactca ctaattaaaa tgaatggcaa tttaataaaa ataagaaatt
     9721 ctgaacttgt tttaggtgtt agagctgtgg ctattagtga gaaaaaaaaa tgtgcggaca
     9781 gtgattttaa ggtgcttggg aatggagttg catcatggtt tgatgaattt gcaagtagaa
     9841 tctttggtga gcggattgat atccttaaaa cgagcgaaat aaaaaagttc ttgtacccgg
     9901 taggtcctgg cattaagttt atccctgaag tttggtttta tgcaaagacc gcaagtagtg
     9961 gatatttgtt tttatatgaa actgagccag ttcgtttatt ctttgatgag cataatcata
    10021 tgagattaag ccgcagtaat ttaagagcgc atgctaaagg tcaccttatt tcaagggctg
    10081 aaatgcttaa ggtaatacct atatctgctt gggcccgcaa tcctattgca ttgataaaaa
    10141 cgatagtgcg tactttgcaa tgcataatat tttgtatttt ggattcaaag aaaaaatgaa
    10201 aatatgtttt tttattggtg gtttaaatag ttcgggtgga acagagcgcg tttcatcagt
    10261 gattgccagc gagctgcaac aacgcggtta ccaaatccac atgctgaatt tacaatgtgg
    10321 cgataagcct ttttttgaat tagctgatgg tattcaagta aatcaactat ttactagcgt
    10381 aggtcgaggt atgctgcgtc tacctctggt gattgttaaa ttacgtcgtt acttaaaaca
    10441 acacaaaatt gatattttaa ttgatgtcga atcgatgttg gctctctatg cattacctgc
    10501 tgtaattggc ttaaatgtac gtcatatctg ttgggagcat tttaattata acgttgattt
    10561 gggcaaagct agccgccgat tagctcgcaa attagcagct cggtttgctg atgatgtggt
    10621 taccttaaca gagcgagata aacaactttg gttagccaac accacttgta aagcaaggat
    10681 aacgaccatt tcgaatccgg tcacgatttc tctgccttca gaaatcaatc tccaaaagga
    10741 aaaactattt ttagcagtag gtcggctcac ttatcagaag gggtttgatc tattgttaca
    10801 agcctgggcg caagttgccc ccttataccc agattggcgc ttacgaatag tgggtgatgg
    10861 cgaagataaa gccatgcttg aacaactatg ctgtgaatta aatattgaag ccagtactga
    10921 gctggtgccg aaaaccaaaa atattgctgt ttattatcaa gaggccgcct attttgtaat
    10981 gagttcacgt tttgagggat ttgggttggt gcttgttgaa gctcaagctt atggcttacc
    11041 gattatctca tttgactgtg atgttggacc aagcgatatc attattaatg atagcacggg
    11101 gtggttgtgc cgtcagaatg atgttttttc aatggcacat aagataagga atgcaataag
    11161 tatttataat gaagataatg ataggtatta tagtatgtgt agtgagtcta ttgttaacag
    11221 taggcggttt tcaattgatg taattttgaa tgaatggatt gatttaattg aaaggatatc
    11281 ttgagtaatg tgtaagttat ctgttgaatc ggggatgact tcggcacgat tgaaattagt
    11341 agaacgggat aacttcgcta taactcttct tgttatactg tcattctgta ttgatttatc
    11401 atctccgatt attacatatg gagtacagtt tgttttagtt tttgtattgg ccttttatac
    11461 tatcttcgca agagagagcc cttctattcc aagaggaaac tttgttttat tttttatttt
    11521 aataattact gtaacagtat ttacattgta tcagcatact tatcttgaac atcaagaatt
    11581 attgtttctg caatattttc gagttttgct atggatggcg tgtggttttt tattctatgc
    11641 ctatcttctt agtcaacagt ctgcgacaat agctcgtgct ttaatggtga caattttaat
    11701 aactggagga gcattataca ttcaatatct agtatattat gcgtttgggt atgttattga
    11761 ctattcatta ttgttggggg ggagcgcatc cagaatattt tttttgacat ttaggccttc
    11821 aggttttaca gctgagcctg cagtttattc tgggattatg attggtcttc tgtctgtgta
    11881 ttatatgttt gtacgtagac tgaacatgat tgttatcttt ggtcttataa gtgttgtttt
    11941 aacatcatct attgctgggt tgatgatgtc ggttttatac gttttagtgg tttttttttc
    12001 cagaccatcc ttgggtcgta ttgttatctc tattgcagct ttatttatta tcattggctt
    12061 gttctttccg gtaattcaag aacgctatgt tactaccgtt tcaggtagtg atgcgagtaa
    12121 taatactaag tttgaaacag tgattaatat gtatgagcat agcaatcttc tgttatcagg
    12181 gtatggtttt gttgggaaaa gtgatagcgc accttcgttt tatgaggggc tatatgatat
    12241 gacatttttt ggggctacaa taataacttt tggacttcct ctagggcttt gtatttgttt
    12301 ttttgttgtt tacataatat taacgcagcc ggctgatggg aaaatactta ttttagtctc
    12361 tttaattaaa ctctcctcgc caatgtgtat gttttttaac ctatttgttg cgttggtttt
    12421 tgttgttaga tataaagcaa gacttaatat tttatcggtg tgataatgca tatattatta
    12481 gttattactg gtctcggcat gggcggagct gaacgtcagg tgtgtgatct tgccgatcag
    12541 ttttctaaga gtggccatag tgtattattg atatctctaa caggtgaagt tattaaccga
    12601 cctcaatcta acaatgttga ggttgtaaat attgaaatga aaaaaacacc tgtcgggttt
    12661 actaaagcct attgccgggt tcgtcaattg atcaaagcat ttgctcctga tgttgtgcat
    12721 agtcacatgg ttcatgctaa tttgtttgct cgatttttga gattgactgt gagcatgcga
    12781 aaattgattt gtaccgcgca caacactaat gaaggagggt gggttagaat gcttgcttac
    12841 cgaatttctg atagtttgtg cgatattagt acaaatgtta gtcaggaagc ggttgatgca
    12901 tttatatcac ataaggctgt accagctaat cgaatgctgc ctatgcataa tggtattgat
    12961 actgaccgtt ttttatttga ttcatcaatg cgtaaaaaat taagaaagaa actaaattta
    13021 acagactatg attcgttgat tttggctgtt ggaagattaa atgctcaaaa agattatcca
    13081 aatttattaa ccgcttttag tcttatagtt tctgtgaatc ctgaaattca attggctgta
    13141 attggtgtcg gggaagagaa atcagtttta acatctatgg ttgatgatct tgggctaaac
    13201 aaacatgtcc attttttagg acttcagcat aacgtcaatg agtggcttag tgctgccgat
    13261 gtgtttgtcc tttcctctgc gtgggaaggc tttggattag tcgttgcaga ggcgatggca
    13321 tgtaagcgag tcgtagtggc taccgattgt gggggggtta aagaggtttt aggtgattgt
    13381 ggtttcttag tccctcccca aaatagtcag gcattagccg atggtttatc ggcggctctt
    13441 tcgttgacga gtgacgaggc gagggtaaaa ggtgaattag ctcggcagag agtgatcgag
    13501 cattattcat tgcaggagca agcaaaacgt tggctagagt tatatcaacg ctgagtattg
    13561 ttttctctgg attatgtata tgctgttttg agcgttttat tgacatatca gttaatggct
    13621 ttaaccttcc acactaattc ttcacctctc ttacaaatcc ccgctaggcg gtacatcgtg
    13681 accgccttta ccctgatgtc tgctctttaa caaacaggac atcagtgtat gtttaaacct
    13741 tttagcgccg aatttttcgg tactttctgg ctggttctgg gtggctgtgg tagcgccttg
    13801 atctctgctg ctttccctca gttaggcatt ggctttttgg gcgtggcgtt ggcttttggt
    13861 ctgacggtag tcaccatggc ttatgcggtc gggcatatct ccggagcgca ttttaacccc
    13921 gcggtgacct tgggtctgtg ggccggtgga cgcttccctg cagcgcgcgt gttaccttac
    13981 atcatcgctc aggttatcgg cggtattgcc gctgcggcag tgctgtatgg tatcgccagc
    14041 ggtaaggctg ggtttgatgc gacaaccagc ggctttgcag ctaacggcta tggcattcac
    14101 tcaccaggcg gttatgcgtt aagcgcctgt atgctgagcg agtttgtcct cagtgctttt
    14161 tttgtcatcg tgatccacgg ggcgacagaa aaacgcgctc ctgcgggctt tgcgccactg
    14221 gcgattggtc tgacgctgac tatcatccat ctggtgagca tccctgtcac caatacctca
    14281 gttaacccag cgcgcagtat cgcggcggcg gttttccaag gtacttgggc attagatcag
    14341 ttgtggatgt tttgcctgat cccatcatta ggcggaattg ccggtggtct gatttaccgc
    14401 gcattgctgg cgcgtccggc tgaagcataa
//
