LOCUS       KY710685               16493 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus vulgaris strain CCUG 14635 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710685
VERSION     KY710685.1
KEYWORDS    .
SOURCE      Proteus vulgaris
  ORGANISM  Proteus vulgaris
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 16493)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16493)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16493
                     /organism="Proteus vulgaris"
                     /mol_type="genomic DNA"
                     /strain="CCUG 14635"
                     /serotype="O1"
                     /culture_collection="CCUG:14635"
                     /db_xref="taxon:585"
                     /collected_by="Cultures Collection, University of
                     Goeteborg, Sweden"
     gene            1..1184
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..16493
                     /note="O antigen gene cluster"
     CDS             1572..2816
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99318.1"
                     /translation="MIINNTKREISSGVYIFISILTLSLNALLFIPLIIKGIITKQKS
                     TPLFTAIFFSLISYYFIPPEEFDLYRHYESFEYFSLNSEPLHKLKDLYLLSLFNIGEY
                     LNIKKEFLYATSVIIYYYIITSVLISLLKINNIRDKKLILILSFLYLFSNTLVEISGL
                     RFTTGLAFSILFLHKYFIVKNKKISIYIYMLLAILSHFSLIIIPITILIYKLFYKLFF
                     DKKFSIFLLFFTLLLGLFFINDLISILIINVEDITGLYIGAEAYTNGAWGVNRLDIQK
                     YNSTGFFVEKSKLYITILISLIISILLIFSKENYLNSLSKYLILLTSISFLLTNFDTI
                     YFRYQSLLTIITLMIITTNKINYKKLNSGSIILILLYIKTLHQFSLGFISTYLNYLSG
                     ISYIDVINNSNVISFFIYIFNF"
     CDS             2831..4096
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99319.1"
                     /translation="MNLIKTSFLSLFATSIKILSALFINKALSLFIGPSGIATIGQFQ
                     NVIQIAATISQGGILPGITKYTAEYHSISNNKNLNLLWSSAFRIILLLSTIVSVVLIT
                     FSEKLSIYILNDKSYTIYIALLGISIFFITLNSFFLSIINGLKQIRFYIFINITQSIF
                     MLIYSIVLIYLFGLKGALFALVTNQAVIFMILIIKIRNKFNINLKYNHIEGKKLLLFS
                     TMALTSAIVGPASLIAIRTYLTHKISLDASGYWQSMWYISSMYLMVITTTLSIYYMPR
                     LSEITKQKELIKELYTGLMFITPLLIIMSSTIYILRGFIIELLFSKEFIQMEILFKWQ
                     LFGDILKIISWLFSYVLIAKAKAKLFIITEILNFTFFSIASFMLINYFGLIGVSYSYF
                     ISNLVYLIIVSTIVYKVFLTKNSTNQYTT"
     CDS             4093..5226
                     /note="orf04"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AXY99320.1"
                     /translation="MNNVIIIAKDSAPTTDPAGLVVYRYALSISNLGINTKLYSILNR
                     KSKHKQILPNWLLSITSKYLNVINKDNIFTLLFSLIIDIRNKVKKNEKIYLITHTNPL
                     YSHWIGIVLKVIFHKNIVWISSFTDPYAKSPFEKKKLRTIGKLIRIIEQKITFSLCNK
                     IIFVSDLMCQYICDKNKVAKEKSIIIPFYYNECWKLEIKNRINNDNDNDKIKIVHTGN
                     IYGNRDASLFFKALSYFENEIDFVNFGKINNLPSDMLLPSNIIINDSIPYENVLKEIH
                     QADYILIIDSFFKEIKNPYIPSKIIDAMYLDKKIIGITDKETELYKFLLKTENIIIEN
                     KFQPIYEELKRIIKKTSYKTKDYSQYSDNKISYIFETIFFQDN"
     CDS             5227..5706
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99321.1"
                     /translation="MKIVLVGYNPRNIGGIESFSRNLKESVFPELHFLYEYDQKGPFE
                     VNDFIGVCKYNFFNRFLNKISRGLYTKNKIKSYLKSKEFDLILLNTPKYLDIIDDLSK
                     VILIQHTTVDNWWLSEYKFNKSNKLLSLAKKVNKIISLSEEEKKQIINKFHIDKNKI"
     CDS             5898..6266
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1_2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99322.1"
                     /translation="MLAEKYSNVKIHNATTEKLKIFDENHIYILSSDFEGYPVSLIEA
                     ASRKLPLIIRNTFPSATEIVNHNGILLKKDWNQSEFCEAVHDIYHNYYKYSESSGEIY
                     NKHSPLKIKQQWLLLTKLSE"
     CDS             6270..7283
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99323.1"
                     /translation="MENNIDVVIYWVDGADPEWRQSYKKYHGTNPGRFRDLGTLKYVL
                     RGIQYNLPWVRYIHFVTNGQLPSWLNINHPKIKFHTHEDIFLFKDSLPVFNSSAIEIN
                     FSNIKGLSEKFILFNDDMLVLNYLPEERFFIKNLPVDYLKLSYPRKGLLYKKIKPQNY
                     LASRFINNAYNFIKNKSIKSINKNSLFNKNYIKKTNFNNLIFSIFKEVYWIDIYHHPQ
                     PHLKSTWDKFIQENKNGIIKKTIHSKFRAPSDINQYIFRFINLLNNDFYPQQYMDNLS
                     IYVRDINKFINKGVTPFNYSLLCICEDESISDSEFNILKDYLTCRLNEKLPYKSDFEF
                     SEL"
     CDS             7308..8345
                     /note="epimerase/dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /gene="fnlA"
                     /product="fnlA"
                     /protein_id="AXY99324.1"
                     /translation="MFASKTLLITGGTGSFGNAVLRRFLNTDIKEIRIFSRDEKKQDD
                     MRKLFKSDKLKFYIGDVRDYQSVANAMRGVDYVYHAAALKQVPSCEFYPLEAVKTDVL
                     GTENVLEAAITFGVKRIVCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLEHTNTTIC
                     CTRYGNVMASRGSVIPLFIRQLMNNEAITITNPEMTRFMMTLDDAVDLVLHAFKNGQN
                     GDIFVQKAPAATINTLVKALLEITEKPDHKVNIIGTRHGEKQFEALCSREEMFVAQEQ
                     GNYYRIPADNRDLNYSKYFEKGEKDLAIVEDYNSHNTQRLDVNGMKELLRKLDFIREI
                     ESGNIIIPEGV"
     CDS             8346..9221
                     /note="dTDP-4-dehydrorhamnose reductase"
                     /codon_start=1
                     /transl_table=11
                     /gene="qnlA"
                     /product="qnlA"
                     /protein_id="AXY99325.1"
                     /translation="MLNIYILGASGMLGYSIFTNLSEYKGFNVFGTVRNIRKKENYFS
                     KFKNKIIENIDISDLSSLEILFQKNKPDIVINCIGLIKQHSISKQYIQAIKVNALLPH
                     ELAHICDNYQAKLIHFSTDCVFDGKSGNYQQTDIPTATDLYGRTKHLGEVTYGKHLTL
                     RTSIIGHELSSSVSLVDWFLNQKDQVNGYTKAIFSGFPTCYIAKLLAEKIIPNKNLNG
                     LYHLSSEPIDKYTLLSNIAAIYHKNIIINPSDELVIDRSLNSTPLRNELDFNSPSWNE
                     LIKYMYEDYNKRYKK"
     CDS             9218..10345
                     /note="UDP-N-acetylglucosamine 2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="qnlB"
                     /product="qnlB"
                     /protein_id="AXY99326.1"
                     /translation="MKKLKIVTIVGTRPEIIRLSRIINKLDNYCEHTLVHTGQNYDYE
                     LNQIFFDDLEIRAPNIFLECAGSSAAETMAQVISKSDKVFAEIKPDAILILGDTNSAL
                     SAISAKRKKIPIFHMEAGNRCFDLRVPEEINRKIVDHISDVNLPYSDIAREYLLREGI
                     SPDFIVKTGSPMDEVLEFYKDKITSSNILKNLNLSAEKYFLVSMHREENVDSKENIRN
                     FVDTLDSLAKKYNFPIIISTHPRTRKKIEELKLSFNPLVHLMKPLGFSDYVHLQTKAK
                     VVLSDSGTITEESSILNFPAINIREAQERPEGFEEGAVMFTGMNTERILQAISILENQ
                     PRGNLRLINKVNDYIAPNVSEKVLRTIISYTDYVNRVIWKK"
     CDS             10333..11559
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99327.1"
                     /translation="MEKVKLKIALIIDDYLPHSTRVGAKMFHELALELKYHGHEITII
                     TPSKDQPSNLYIDKLDDITIWRFKNGPIKDVKKIQRAINETLLSYNAWKAIKHLINKD
                     SFDGIIYYSPSIFWGKLVNKLKKKCQCPSYLILRDLFPQWIVDAGILKQGSLIEQYFR
                     FFERYSYKQASIIGLMSQKNLDYFYQHNKNKGLKCEILRNWANTTQTNIDTNGYISIR
                     ESLNLQNKTIFFYGGNIGHAQDMGNLMRLVKKMSAYPDAHFLFLGQGDEVELIKTLSN
                     KWNLNNFSYLPSVDQTKFKLILKEIDIGLFSLASHHSAHNFPGKLLGYMANHIPILGS
                     VNEGNDLQELVNKENAGFIHINGEDDNLFESAKKLHLDIELRKKCGNDAYNLLCKEFS
                     VTSIASTLIGRLRSLK"
     CDS             11556..11957
                     /codon_start=1
                     /transl_table=11
                     /gene="wbuC"
                     /product="wbuC"
                     /protein_id="AXY99328.1"
                     /translation="MKYIDNSSLENLYKEAKKSERLRSHLLSHKSHEDKVQRLLIALV
                     KDSYVEPHYHELPHQWEMFIILEGQIEITLYNHSGEIKEKFLAGENTNISIVNLEPNE
                     IHALKCISQKALMLEVKEGPFDPNYAKKFLI"
     CDS             12018..13184
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99329.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFFSNQRARFTATLDKEFAYKDADFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLSQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESYGLNSKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLNEFKAISDVIITNRMSSVLNDVEEKVYTRDLFGND"
     CDS             13196..13699
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99330.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(13775..14332)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXY99331.1"
                     /translation="MALTEVTFSELKACLHAEYIGDNKPFSWIRLFHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFLKKLGIYIGAKNNRKYCNDISLRTRIGEGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKDGKVDIRVGNHVYIGCNSSIIGGEIEVGDNAVIGAHA
                     LVLKDVGEGCRYLTKVVAEIKQLSM"
     CDS             complement(14332..15165)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99332.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDDSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWGQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHAVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(15269..16285)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99333.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDAEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     STLF"
     gene            complement(16285..16485)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5920 a   2652 c   2542 g   5379 t
ORIGIN
        1 tttggtggcg tcgattaatt cgtgccattg ataaatgggc ccctcctggc cagcgtttaa
       61 ttatcgtaac cagtgaagga cgtattatcg gcgctcaacg taatgaaatg caggttgtca
      121 gaaactttat tggccaatct gataacgccg atcatcctaa aaagaaaaaa tatggtcgtt
      181 ctgagatgtt agggccattt tcagtaaggg atggtgaaga tcattatcag ctttatcttg
      241 tacgtccatc aagtagccct caatctgact ttatcaactt attatttgat aaaccgttat
      301 tgctactcat attcactatg cttattagca caccgttatt agtgtggctt tcttggagct
      361 tggcaaaacc ggcaagacaa cttaaaaacg cagctgatga tgttgcgaaa ggtaatttac
      421 gccctcatcc agagctagaa accggcccac aagagttttt agctgctggt actagtttta
      481 atcagatgat aagcgcactc gaacgcatgg ttgaagcaca gcaacgttta atttctgata
      541 tttctcatga attacgaact ccattaacac gcttacaatt agccagtgca ttgttacgtc
      601 gccgcaacgg agaaagtaaa gaattagaac gcattgaaac tgagactcaa cgccttgatg
      661 ggatgattaa tgacttattg gtgctttcac gtaatcaata taaaaatgag ttgttacgag
      721 aaactgtaaa agcaaatgag ttgtgggatg atattttaga taacgctaaa tttgaggctg
      781 aacagagtaa taagacatta caagtgacaa cacctccagg tgcatggcct atttactgta
      841 acccttactc actcgctagc gcatttgaaa atattgttcg taatgcctta cgttattcaa
      901 attctcgcat tgaagttgca ttcacagagc aaaatcaagg catcaccatt attgttgatg
      961 acgatggccc aggtgttagc cctgaagaca gagagcatat cttccgaccg ttttatcgca
     1021 ctgacgaagc aagagatcgc gaatcaggtg gtacaggctt aggattagcg attgttgaga
     1081 cagcagtaag tcaacatcga ggccatgtta aagccgatga cagcccatta ggtgggctga
     1141 gagtagagat ttggttgccg aggggaagtg ccggtaataa atagccaata gcctagatag
     1201 agtaattaaa ctgttgtatc aaaatatcct gccatctatt gaagaatagg tggcatattc
     1261 tttttgttag catctaatga tgcaattcag aatgatctgg ctgaatcgac cgctattccg
     1321 atatttatat atctacaatc aatgctaaaa tacccagcta ttacttgacc gtctggtttg
     1381 aagagtctac tctcagtgcg gtttttcgca tattgttaaa atgtgcttat ataataaaac
     1441 tcttatatta caaaaagata agggtttttc gagataagtt ttggcgctct gggggccgta
     1501 aacccagtgc ggtagctata ctactaattt tatattaata aatgcttata acactctttt
     1561 aatgaggtat aatgataata aataatacaa aaagagagat tagctctggt gtttacatat
     1621 tcatatccat actaactctg tcattaaatg cattactatt tattccttta ataataaaag
     1681 gaataataac aaaacagaaa agtacacctt tatttacagc aatttttttt tcgttaatat
     1741 catattattt tattcctcct gaagagtttg atttatatag acactacgaa tcatttgaat
     1801 atttttcatt aaatagtgaa ccactacata aattaaaaga tttatattta ttgtcattat
     1861 ttaatattgg tgaatactta aatataaaaa aagaattttt atatgccaca tctgtaatta
     1921 tatattatta tataattact agtgttttaa tctcactatt aaaaataaat aatataagag
     1981 ataaaaaatt aatcttaatt ttaagttttt tatatctttt tagtaatact ctagttgaaa
     2041 tatcagggct aagatttaca actggactgg ccttttctat tctattttta cacaagtact
     2101 ttatagtaaa aaataaaaaa ataagcatat atatatatat gcttttagca atattaagtc
     2161 atttttcatt aataataata cctataacga tcttaatata taagttattt tataagttat
     2221 tttttgataa aaaattttct atatttctac ttttttttac tttgctactc ggtttatttt
     2281 ttataaatga tttaatttct attttaatta ttaatgttga agatattact ggtttatata
     2341 ttggtgctga ggcatatact aatggtgctt ggggagtaaa tagattagat attcagaaat
     2401 ataattcaac gggttttttt gttgagaagt ctaagttata tataacaata ttaatttctc
     2461 taataatttc tattcttttg attttttcaa aagaaaatta tttaaatagc ctttcaaaat
     2521 atttaatact tttaacatca attagttttt tattgactaa ctttgatact atttatttta
     2581 gatatcaatc tttattaaca ataattacac tcatgatcat tacaacaaat aaaataaatt
     2641 ataaaaaatt aaattcaggt tcaataatat taatattatt atatataaaa acattacatc
     2701 aattttctct aggctttata tcaacttatt tgaactattt atctggaatt agttatattg
     2761 acgtaataaa taattctaat gtaatttcat tctttatata tattttcaac ttttaatcgg
     2821 taaaattact ttgaatttaa tcaaaacttc atttctgagc ctatttgcta catcaataaa
     2881 aatacttagt gcattattta ttaacaaagc actttcacta tttattggtc ctagtggtat
     2941 agcaacgata ggtcaatttc agaatgtaat acagatagca gctacgatta gtcaaggagg
     3001 gatactccct ggtatcacca aatataccgc tgaataccat tcaatatcaa ataataaaaa
     3061 tcttaattta ctgtggtctt ctgcgttcag aataatattg ctattatcaa caattgtaag
     3121 tgttgtttta attacttttt ctgaaaaatt atctatatat atattaaatg ataagagtta
     3181 cactatatat atagcattat taggtatatc aatatttttc ataacattaa attcattttt
     3241 cctatcaata atcaatggat taaaacagat tagattttat atttttataa atataactca
     3301 aagtatattt atgttaatat attccattgt attaatatat ttatttggat taaaaggggc
     3361 cttattcgct ctcgtcacaa accaagctgt tattttcatg attttaatta taaaaataag
     3421 aaataaattc aatataaact taaaatataa tcatattgaa ggaaagaagt tattattatt
     3481 ttctactatg gcattaactt cagctattgt tggccctgca tccttaatag ctatcaggac
     3541 ttatcttact cataaaatat ctcttgatgc atcaggttat tggcaatcaa tgtggtatat
     3601 atcatctatg tatttaatgg taattacaac tactttgtct atttattata tgccaaggtt
     3661 atctgaaata acaaaacaaa aagaactaat aaaagaacta tatacaggat taatgtttat
     3721 tacaccactg ctcataataa tgtcatctac tatatatatc ttacgaggat ttattataga
     3781 gttactcttt tccaaagagt tcattcaaat ggaaatatta ttcaaatggc aactctttgg
     3841 tgatatatta aaaattatat cttggctatt ttcatatgtt ctaatagcta aagctaaggc
     3901 aaaattattt attataacag aaattttaaa ctttactttt tttagtatag catcatttat
     3961 gttaattaat tattttgggc taattggtgt atcttactct tattttatat ctaatcttgt
     4021 atatttaatt atcgtatcaa caattgtata caaagtattt ttaacaaaaa attcaactaa
     4081 ccaatatact acatgaataa cgtaattatt atagctaaag attcagcccc cactacagat
     4141 ccagcaggat tagtagtcta tcgctacgct ctatccatct ctaatctagg cattaatact
     4201 aaactatatt ctattttaaa tagaaaaagt aaacataaac aaatattacc taactggtta
     4261 ttgagcataa cctcaaaata tctaaatgta attaacaaag ataatatttt tacattatta
     4321 ttttcattaa taatagatat tagaaataag gtaaagaaaa atgaaaagat ataccttatc
     4381 acacatacta atccgttata cagccattgg attggcattg ttttaaaagt tattttccat
     4441 aaaaatatag tatggatttc atcttttact gatccatatg caaaatcccc ttttgaaaaa
     4501 aagaaactaa gaaccatagg gaaattaatt cgaatcatag aacaaaaaat aacattttct
     4561 ttatgtaata agatcatttt tgtttcagat ctaatgtgcc aatatatctg tgataaaaat
     4621 aaagttgcaa aagaaaaatc tataattatt cctttttatt ataatgaatg ttggaaatta
     4681 gaaataaaaa atcgtataaa taatgataat gataatgata aaattaaaat tgtacatacg
     4741 ggtaatattt acggaaatag agacgcatct ttgtttttca aagcgttatc ttattttgaa
     4801 aatgaaatag attttgtaaa ttttggaaaa ataaacaatc taccatcaga tatgctatta
     4861 ccaagtaata ttataattaa tgattctatc ccatacgaga atgtattaaa agaaatacat
     4921 caggctgatt atatattaat aattgactca tttttcaaag aaataaaaaa tccttatatt
     4981 ccatctaaaa ttatcgatgc tatgtattta gataaaaaaa taataggtat tacagataaa
     5041 gaaactgagc tatataaatt ccttcttaaa acagaaaata ttattattga aaataaattt
     5101 cagccaattt atgaagaatt gaaaagaata ataaaaaaaa catcttataa aacaaaagat
     5161 tattcccaat attcagataa taaaatctct tacatttttg agacaatatt tttccaggat
     5221 aattaaatga aaatagtatt agttggatat aacccaagaa atattggtgg aattgagtct
     5281 ttttcaagaa atctaaaaga aagcgtattt cctgaattac attttctata tgaatatgat
     5341 caaaagggtc cttttgaagt aaacgatttt ataggggtat gtaaatataa tttttttaat
     5401 agatttctaa ataaaatatc acggggttta tacactaaaa ataaaattaa gtcttattta
     5461 aaatcaaaag agttcgattt aatattgtta aacactccta aatacttaga tattatcgat
     5521 gatttaagta aagtaatact aatacaacat actactgtag ataattggtg gttatcagaa
     5581 tacaaattta ataaaagtaa taagcttctt tctttagcaa aaaaagtaaa taaaataatc
     5641 tctctttctg aggaagagaa aaaacagatc ataaataaat ttcatatcga taagaataaa
     5701 atttaaggta ataaaactta ccttcaggtc ttgaatatta taataaaata aaagaaccct
     5761 ggtaggaatc tattaatgct aactagattt tcaaaatgaa attaaaagaa ttgatttagt
     5821 tattaatgca atgaaaaatt ttacctgatt ttaatttaaa tatatatgga agtggtaaag
     5881 atgaaaaaga attacgtttg ttagctgaaa aatattcaaa tgtaaaaata cataatgcta
     5941 ctactgaaaa attaaaaata tttgatgaaa atcatattta tatattgagt agtgattttg
     6001 aagggtatcc tgtaagcctg attgaagcag cttcaagaaa actacctcta attattagaa
     6061 acacgtttcc ttccgcaaca gaaattgtta atcataatgg gattttatta aaaaaagatt
     6121 ggaaccagag tgaattttgt gaagctgttc acgatattta tcataattat tataaatatt
     6181 cagaatcatc tggagaaata tacaataagc attcccctct aaaaatcaaa caacagtggc
     6241 tgttattaac taaattatcg gaataatcaa tggaaaataa tatagatgta gtcatttatt
     6301 gggttgatgg tgcagatcct gagtggcgac aatcctacaa aaaatatcat gggactaatc
     6361 cgggtagatt tagagactta ggcactctaa agtatgtgtt aagaggaatt caatataacc
     6421 ttccatgggt aagatacata catttcgtta ccaatggtca acttcctagt tggttaaata
     6481 taaatcaccc taaaataaaa tttcatactc atgaagatat ttttctattt aaggatagct
     6541 tacccgtttt taattcttca gctatcgaaa taaacttctc caatattaaa ggattatcag
     6601 aaaaatttat tttatttaat gatgatatgt tagtacttaa ttatttgcca gaagaacgtt
     6661 tttttatcaa aaatctacct gtcgattatt taaaactatc ttatccaaga aaagggttat
     6721 tatataaaaa aataaaacct caaaactatt tagctagtag atttattaat aatgcatata
     6781 acttcattaa gaataaatct ataaaatcca ttaataaaaa ctctcttttt aacaaaaatt
     6841 atataaaaaa aaccaatttt aataatctta ttttttctat atttaaagaa gtttattgga
     6901 tagatattta tcaccatcca caaccacatt taaaatccac atgggataaa ttcattcaag
     6961 aaaacaaaaa tggaataatt aaaaaaacca tacattccaa gtttagagca ccatcagata
     7021 taaatcagta tatatttaga tttataaatt tattgaataa cgatttttat cctcaacaat
     7081 atatggataa cctatctatt tatgtaagag atataaataa atttattaat aaaggagtta
     7141 ctccttttaa ctattcatta ttatgtattt gtgaagatga atctatttca gattctgaat
     7201 ttaatattct aaaagattat ttgacttgta gactcaatga aaaattgcca tacaaaagtg
     7261 attttgagtt tagtgaatta taacatagaa aaatagaagg tttaattatg tttgcatcaa
     7321 aaacattact tataactggt ggtactggtt cttttggtaa tgctgtatta cgtcgttttt
     7381 taaatactga tataaaagaa attcgtatat tttctcgtga tgagaaaaaa caagatgata
     7441 tgcgaaaatt atttaaatca gataaattga aattctatat tggtgatgtc agagattatc
     7501 aaagtgttgc caatgctatg cgtggtgtag attatgtgta tcatgcagca gcattaaaac
     7561 aagtaccttc ctgtgaattc tatccattag aagcagtaaa aactgatgta ttagggacag
     7621 aaaatgtatt agaagcagca atcacatttg gggttaaacg tattgtttgt ttaagtacag
     7681 ataaagccgt ttacccaata aatgcgatgg gcatatcaaa agcaatgatg gaaaaagtca
     7741 ttgttgctaa aagtagaaat ctagaacaca ccaataccac aatttgctgt actagatatg
     7801 gaaatgtaat ggcatctaga ggctccgtta tcccattatt tattcgacaa cttatgaata
     7861 atgaagcaat cactatcact aatccagaaa tgactcgttt tatgatgaca ttagatgatg
     7921 cagtagacct cgtgttacat gcatttaaga atggtcaaaa tggtgatatt tttgtccaaa
     7981 aagcacctgc agcgacaatt aatactctag tcaaagcatt gttagaaata acagaaaagc
     8041 cagatcacaa agttaatatt attggtactc gtcacggtga aaaacaattt gaagctttat
     8101 gcagccgaga agaaatgttc gttgctcaag aacaaggtaa ctattatcga attccagctg
     8161 ataatcgaga tttaaattat tcaaaatact ttgaaaaagg cgaaaaggat ttagctattg
     8221 ttgaagatta taattcacat aatactcaac gtttagacgt taacggtatg aaagagcttc
     8281 ttagaaaact tgattttatt agagaaattg agtctggaaa catcattatt cctgaaggtg
     8341 tttaaatgtt aaatatttat attttaggtg ccagtggtat gctgggttat agtattttta
     8401 ccaatttatc tgaatataaa ggttttaatg tttttggcac agttagaaat ataagaaaaa
     8461 aagaaaatta tttttctaaa tttaaaaaca aaattataga aaatattgat atctctgatt
     8521 tatcttctct tgaaatttta tttcaaaaaa acaaaccaga cattgtaatt aactgcattg
     8581 gattaataaa acaacatagt atttctaaac aatatattca ggcgataaaa gtaaatgctt
     8641 tattacctca cgaattagct catatttgtg acaattatca agcaaagtta attcactttt
     8701 ctacagactg tgtctttgat gggaagtcag gtaactatca acaaactgat atccctacag
     8761 caacagatct ttacggccga actaaacatt taggtgaagt cacttatgga aagcatttaa
     8821 ctttacgaac atcaattata ggtcacgaat tatcttcaag cgttagccta gtagattggt
     8881 ttttaaatca gaaagatcag gtaaatggat atactaaagc cattttttct ggttttccta
     8941 catgttatat agcaaagttg ctagctgaaa aaataatacc caataaaaat ttaaatggtt
     9001 tatatcattt atcaagtgaa ccaattgata aatatacact attatcaaat attgcggcta
     9061 tttatcataa aaatattatt ataaatccat cagacgagct tgttatagat cgttcactta
     9121 attccacacc tttacgcaac gagcttgatt ttaattcacc atcatggaat gaattaatca
     9181 aatacatgta tgaagactat aacaaaaggt ataaaaagtg aaaaaattaa aaattgtaac
     9241 tatagtagga acacgtccag aaataattcg actatcacga ataataaata agttagataa
     9301 ttattgtgaa catactcttg ttcatactgg acagaactat gattatgaat taaatcaaat
     9361 attctttgat gacctcgaaa tcagagctcc caatattttt cttgaatgtg ctggttcatc
     9421 tgctgctgaa actatggctc aagtaatcag caagtctgat aaagtgtttg ctgaaattaa
     9481 acctgatgca atattaatat taggtgatac aaatagtgcc ctttccgcaa tttctgctaa
     9541 aagaaagaaa atccctatat ttcatatgga agcaggtaat cgttgttttg accttagagt
     9601 accagaagaa ataaatagga aaatagttga ccatatctct gatgtcaacc ttccttatag
     9661 cgatatagca agagaatact tacttcgaga agggattagt cctgatttta tagtaaaaac
     9721 aggaagccct atggatgaag tattagagtt ctataaagat aaaattacat cctctaacat
     9781 attaaaaaat ctaaatttgt ctgcagaaaa atatttctta gtcagtatgc atagagaaga
     9841 aaatgttgac tcaaaagaga atataagaaa tttcgttgat actttagatt ctctcgcaaa
     9901 aaaatacaac ttcccaatta tcatatccac acacccaaga acaagaaaaa aaatagaaga
     9961 gttaaagcta tcatttaatc cattagttca tttaatgaag cctctaggtt tttctgatta
    10021 tgtacatctg caaactaaag ctaaagttgt cttaagtgat agtgggacta ttactgaaga
    10081 atcttcaatt ttaaactttc ctgccattaa tataagagag gctcaggaaa gacccgaagg
    10141 atttgaagaa ggtgctgtga tgttcacagg aatgaataca gaaagaattt tacaagctat
    10201 atccatactt gagaaccaac ctagaggaaa cttacgtcta attaataaag ttaatgatta
    10261 tattgcacca aatgtatctg aaaaagtact aaggactatc attagctata cagattatgt
    10321 taatcgagta atatggaaaa agtaaaacta aagattgctt taatcattga cgattacttg
    10381 ccccatagca ctcgagttgg ggcaaagatg tttcatgaat tagcactaga attaaaatat
    10441 catggtcatg agattaccat tattacaccg tcaaaagatc aaccatctaa tctatatatt
    10501 gataaattag atgatattac tatctggcgt tttaaaaatg gtcctattaa ggatgtaaaa
    10561 aagatccaaa gagcaattaa tgaaacacta ctttcttaca atgcttggaa agctattaaa
    10621 catttaatca ataaggattc ttttgatggc ataatttact attctccttc tatattttgg
    10681 ggaaaattag ttaataaact aaagaaaaaa tgccagtgtc cttcttattt aatattaagg
    10741 gatttatttc ctcaatggat tgttgatgct ggtattttaa agcaaggttc attgatcgaa
    10801 cagtactttc gattttttga acgttactcc tataaacaag catcaataat tggcttaatg
    10861 tctcaaaaaa atctagacta tttttatcaa cataataaaa ataaaggttt gaaatgtgaa
    10921 attttaagaa attgggcaaa tactactcaa acaaacattg atactaatgg atatatttca
    10981 atacgtgaat ctttaaactt acaaaataaa actattttct tttatggtgg aaacattggt
    11041 catgcccaag atatgggtaa tttgatgcga ctagtgaaga aaatgagtgc ttatcctgat
    11101 gctcattttc tctttcttgg acaaggcgat gaagttgaat taattaaaac tttatctaat
    11161 aaatggaatt taaataattt tagctatcta ccctcagttg atcagactaa atttaaatta
    11221 attcttaaag aaattgatat tggtttattt tcattagcgt cacatcactc tgcacataat
    11281 tttccaggta aattattagg atatatggct aatcatatcc ctatattagg tagcgttaat
    11341 gaaggaaacg acttacaaga actagtaaat aaagaaaatg caggttttat tcatataaat
    11401 ggcgaagacg ataatttatt tgaatctgct aaaaagctcc accttgatat agaactacga
    11461 aaaaaatgtg gaaatgatgc ttataatctg ttgtgtaaag agttttcagt tacttctatt
    11521 gcatcgacac taataggtag gttgagatct ttaaaatgaa atatattgat aattcatcgt
    11581 tagagaattt atataaagaa gcaaaaaaat cagaaaggtt acgttctcat ttattatcac
    11641 ataaatccca tgaagacaaa gtacaacgct tacttattgc tttagtaaaa gatagctatg
    11701 ttgaaccaca ttatcacgag ttacctcatc aatgggaaat gtttattatt ttggaagggc
    11761 aaattgaaat tacactatat aaccattctg gagaaataaa agaaaaattc ttagctggag
    11821 aaaatactaa tatttcgatt gtaaacttag aaccaaatga aatacacgct cttaaatgca
    11881 tctctcaaaa agcattaatg ttggaagtaa aagaggggcc ttttgatccc aattatgcta
    11941 aaaaattctt aatttaatag atcaatttac tatctataat acacctataa agttaggtta
    12001 atcttaaagg tctatttatg aaaatcacaa tctcaggtac cggctatgtc ggtctatcta
    12061 acggaatact cctttcccaa aacaacaaag ttgttgcgtt agatatcaat aaagaaaaag
    12121 ttgaattact gaataaaaaa atctctccaa ttacagataa agaaattgaa gaatttttta
    12181 gcaaccaaag agctcgattt actgcaacct tagataaaga gttcgcttat aaagatgcgg
    12241 attttgttat cattgctaca cctaccgatt acgatcctaa aacaaattac tttaatacct
    12301 cctctgtaga gtctgttatc cgtgatgttt tagaatataa tccaaacacg acaatgatta
    12361 ttaaatcaac cattcctgtt ggttttacga aagaaatgag agaaaaatat aatacggata
    12421 atattatttt ctctcctgaa ttcttacgtg aaggtcgtgc actttacgat aatttatacc
    12481 catcgcgtat tgttattggt gaacaatcag aaagagcaca aaagtttgca gacctttcac
    12541 aacaaggtgc attaaagaaa gatattgatg ttctgttcac tgactcaaca gaagcagaag
    12601 ccattaaatt atttgccaat acttatttag cgctacgtgt tgcttacttt aatgaacttg
    12661 atagttatgc tgaatcttat ggtttgaact caaaacaaat catcgaaggc gtctgcttag
    12721 atcctcgtat cggcaaccat tataataacc catcattcgg ttatggcggt tactgcttac
    12781 caaaagatac taaacagctg ttagcaaact acgactcggt gcctaataat attattagtg
    12841 caatcgtcga agctaaccgc acacgtaaag actttattgc cgactcaatt atcgctaaat
    12901 cgccaaaaat cgtaggtgtt tatcgtttaa ttatgaaatc tggctctgat aatttccgtt
    12961 cttcttctat ccaaggcatt atgaaacgca ttaaagccaa agggattgag gtggtaatct
    13021 atgagccaga gatgaaagaa gagactttct ttaactcaaa attaattcgt gatttaaatg
    13081 agtttaaagc aatctctgat gtcattatta ctaatagaat gtcatctgta ctaaacgacg
    13141 ttgaagaaaa agtttatact cgtgacctct ttggtaacga ctaaatcgat tttatatgct
    13201 caatatcgtc ttatttgaac ccgaaattcc accgaatacg ggtaatatta tccgcctgtg
    13261 tgctaacaca ggctttcatc ttcatttgat ccaacctttg ggttttactt gggatgataa
    13321 acgtcttcgt cgtgcaggac ttgattatcg tgaatttgct gatattaaac agcatcacga
    13381 ttactatgct tttttagaaa gtgaaggttt aaaccctgat aatgcacaat caccaacagg
    13441 tgcgcgtgta tttgcgttaa cgacaaaagg cacaccagca catagcaatg tgagctatca
    13501 agatggagat tatctccttt ttggacctga aacccgtggt ttgccacctt atgtgttaga
    13561 taatatgcca acacagcaga aaatcagagt tccaatgctt gctgatagtc gcagtatgaa
    13621 cctgtcaaat accgttgcag ttgtggtttt tgaagcatgg cgtcaacttg gttatcctgg
    13681 tgcgttatta agagactgaa atgcaaaaag cgctgatcat aaataatgag caagcgcttt
    13741 tttaatttca aatgactaaa aacttaattt attattacat tgagagttgt tttatttcag
    13801 caacaacctt cgtgagatac ctacaccctt cccctacatc cttcaatacc aatgcgtgag
    13861 caccaatcac tgcattatca ccaacctcta tctcaccgcc gataatcgaa gaattacaac
    13921 caatataaac gtggttgccg actcgaatat ctactttacc atccttttct tttactccga
    13981 tggtcacacc ttgcattaac gtcacattct cacctaattg acagtaatta gtcagcacaa
    14041 taccgatagg atgtggtaat gaaaggcctt ctcctatgcg cgttcttaat gagatatcat
    14101 tgcaatattt acgattgttt ttagcaccaa tgtaaatgcc taattttttg agaaaacgat
    14161 ttttagagaa ataaaaaaat tgagcgacac gccaccaaaa cagataacgt gaacgttggc
    14221 agaaaaagac gcgatgaaaa agtcttatcc aactaaaagg cttgttatca ccgatatatt
    14281 cagcgtgtaa acaagcctta agttcagaaa atgtaacttc tgttaatgcc attaaatgcc
    14341 atcaccacat tcaaaaccag gtactacacc attaaagtgt tgatccatat cgagtgaagg
    14401 tttatcactc gtcggcttac caacaatgcg agcaggaacc cctgcaaccg tggtatgtgc
    14461 aggcactgca tgtaatacga cagatcccgc accaatttta gcaccgcgac caatctcaat
    14521 attgccgagt attttagcac ccgcaccaat catcacacct tcacgcactt taggatgacg
    14581 atcaccacag gttttacctg ttccgcctaa agtcacagat tgtaaaatag aaacatcatt
    14641 ctcaacaaca gctgtctcac caattacaat gcctgttgcg tgatcaagca taataccgtg
    14701 gccaatacgt gcagctgggt gaatatcgac accaaacgta acagaaatct ggttttgtag
    14761 gtaaacagca agcgctttgc gatcttgtcc ccataaccaa tgtgctatgc gataagcctg
    14821 caacgcatga aagcctttta aataaagcaa cggtgtggag tatttatcaa ctgcggggtc
    14881 gcgtaaacga acagctgaaa tatcataagc cgcagattcg atcatggaat catcgctacg
    14941 atacgcttct tcaacaatct cacgaacggc aatcgcaggc attgtctgtg tcgccagctt
    15001 attcgctagc atataactta aggcgctacc taagttttca tgtttgagta atgtcgcatg
    15061 gaagaaactc gccaaaatcg gctcgcaatc agccaaacat cttgcttcat ttttaatatg
    15121 atcccagact ctttttagtt cttctagcga catactcact ctcattcttt ttacattgaa
    15181 acctaagtaa caacttaagc ttcttatgtt gaaaaccgaa acagacactt taaaccaatg
    15241 cgttaagtag cgattattta aaatagactt agaacagagt gctgtctatt tcatcttttg
    15301 ttgcgcgacc taataaagat agtgccgcct ctttcgcgtc tttatgttgg tataaaattt
    15361 ggtagatctg ctcagtgata ggcatttcaa caccgacttg ttctgctaag gcgcgaactt
    15421 ctttagtgtt ccgataacct tcaaccacct gcccgatttt ctcttgggca gtatcaacat
    15481 caagcccttg gcctaacatc ataccaaatc ggcgattacg ggattggtta tcggtacaag
    15541 ttaaaacgag atcgcctaac ccggccattc ccataaacgt cgcagcatct gcacctaatg
    15601 ctttacctaa acggctcatt tcagcaagtc cgcgggtaat tagtgctgta cgtgcattcg
    15661 cgccaaaccc cataccatca gacattccag caccaatagc aatcacgttt tttaccgcgc
    15721 cacctaattg cacaccgata aagtcaggat ttttatacac acggaaactt ttaccacaat
    15781 ggaacaactg ttgaagctct tcagaaaatg tattatctgt tgatgcaaca gatatcgcag
    15841 taggcagacc tgctgcaagc tctttggcaa aagtaggacc agataaaact gcgagcggga
    15901 tttcattacc taatacttca cgcgccacat cttgtaacaa gcgaccagta tgagcctcaa
    15961 gtccttttgt cgcccaaaca acacgtgcat cctgacgtaa aaagggtttg atctgctgta
    16021 atacgtcacc aaaaacatgg cttgggatca caaccagaat attacggcta gcttcaatgg
    16081 ctttttgcaa agaagcttcc atgtataaac tatcaggaaa agaaacatcg ggcagaaatg
    16141 cctgattaca acgtgcttgt tctaacgccg caacatgttc agcatcatga ccccaaagta
    16201 caacatcatg gccattgcgt gctaaggtaa tcgctaaagc ggtgccgtat gaaccggcac
    16261 cgataactgt catagaagcg ttcattaagc ttcttcagcc ccttcattag cagcatcagt
    16321 ttgttgctgt tgtaaatagt tcataaacaa cgcatcaaag ttaactggtg ctaagttcag
    16381 ttgtgggaaa gtaccacggc tgactaagtt agtgatacat tcacgagcat aagggaacag
    16441 aacgttaggg caatatgcgc ctaagcaatg tgctaattga gtgcctcaaa cct
//
