LOCUS       GU254060               15098 bp    DNA     linear   BCT 14-AUG-2010
DEFINITION  Proteus mirabilis strain Prk 20/57 O antigen gene cluster, complete
            sequence; and WemP (wemP), CysE (cysE), and GpsA (gpsA) genes,
            complete cds.
ACCESSION   GU254060
VERSION     GU254060.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 15098)
  AUTHORS   Wang,Q., Torzewska,A., Ruan,X., Wang,X., Rozalski,A., Shao,Z.,
            Guo,X., Zhou,H., Feng,L. and Wang,L.
  TITLE     Molecular and genetic analyses of the putative Proteus O antigen
            gene locus
  JOURNAL   Appl. Environ. Microbiol. 76 (16), 5471-5478 (2010)
   PUBMED   20581173
REFERENCE   2  (bases 1 to 15098)
  AUTHORS   Wang,Q., Torzewska,A., Ruan,X., Rozalski,A., Shao,Z., Guo,X.,
            Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..15098
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="Prk 20/57"
                     /db_xref="taxon:584"
                     /note="serogroup: O10"
     gene            535..1428
                     /gene="wemI"
     CDS             535..1428
                     /gene="wemI"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32291.1"
                     /translation="MNKYLKIIRNKLRYKYCDVFPKLITKSIYKERMNKKLDLRHPQT
                     FNEKLQWLKLNLYRDNPLVTQCADKYAAREYVKECGCQEILNEIHQVWEEPHEINFSE
                     LPNKFVLKCNHGAGYNIICRDKNSISPDKIKQKLSTWLNEDYWRLSVEFVYKDVPKKI
                     ICEKFIETKNNELPYDYKLFCFHGKPLFVMICTERESHHPKFYFVNKEWELLPYGIDY
                     KNRNVSTVLEKPQNYQKLFEYAEKLSKPFPFVRVDLYLNNGIIHFGELTFIPSAGMDK
                     ELNNEEVKNVDMIFGNYLKLN"
     misc_feature    535..12382
                     /note="O antigen gene cluster"
     gene            complement(1440..2429)
                     /gene="wemJ"
     CDS             complement(1440..2429)
                     /gene="wemJ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32292.1"
                     /translation="MKNHPKVSVLMSVYNDAVHLEEAINSILNQTVKDLEFIIIDDGS
                     TDVSVDILRRYEKQDSRVKVYTNESNIGLAKSLNKGFLLSSGEYIARMDADDYAYPIR
                     LQKQLEFMEAHKNIMVCGTSMEIYEDHNKFMVPPTAHNAIAAGLIFGTNFYHPTVMFR
                     ASFIGDNQITYPEDYKYAQDYGLWVKLLKLGKKDIFANLEEPLLKYRTHPEKNRKKYF
                     NTQTSFAIKTQISALEIFSINANEKVLYDLNFSSEYLTLDNIKKCNSLLEKIRIKNIQ
                     SKPYFYKELKVAIIRKKVSLFRRCEKKSFYYPYMKLKARFIYLINRKIIKSIN"
     gene            3039..3821
                     /gene="wemK"
     CDS             3039..3821
                     /gene="wemK"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32293.1"
                     /translation="MQNIESTKVSIIISAYNAEKYIHESLESIINQTHKNLEIIVIND
                     GSTDNTKHICTEFQKRDPRIIFIDKENEGLTKSLNKGIHLATGTWIARQDADDISLEN
                     RIEEQLKFCIKNKIDFCTSQAIQFSAQNNLRLIPKIRSSIAFKKKILKYGNPHIHGTF
                     FFRKKIIEKINYDEEIKYAQDFNFILAVKHAKFNMALLKTPLYKLRIHNNSISSNKKK
                     EQIEAVKTSLEKYRYSTHYIIEENNGLKKQALRIMKQCESII"
     gene            3821..5041
                     /gene="wzx"
     CDS             3821..5041
                     /gene="wzx"
                     /note="O unit flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ADL32294.1"
                     /translation="MKKLIYDIIYKFSQGGIKHLLQLIILFYGSSHLGADNFGEYIYI
                     TTIIVTLSLFLDFGLSTTLMKEIAENKGNEEKIYRSIIPYFFVLTSLSGIIFFVLSNL
                     EFNLKILLGITLSIMPICSIFDGIHRGKLEFKINAKYNFLSALLCLFLIFPLVYYLKI
                     AGLLLLNLIFYSSLLLQYIYNCRKKIFSKVSFDKKYLLKVTSYSIIIGIGFVGQFLYT
                     KADIIILGKYNYLSEITTYEIFDRLLMYCILPFGLIAQVLAPRVINTNIDKIKKLTNI
                     SFCISIMLSITLYLLSSYILKNNYNTQLLGIPYSTYSSIIPLLIACAPIRIFAILQTQ
                     ALIIPLGFAKIVASTTLFFGIINVILDFSLLYLYGFIGIFYSTLIVVSLNTLSQYILF
                     GNKFKWKKTGLSKP"
     gene            5011..6183
                     /gene="wemL"
     CDS             5011..6183
                     /gene="wemL"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32295.1"
                     /translation="MEKNWSKQALIFINSLKNDNGSYSFSKKSSCASCLSTSFALLAL
                     EVLQQKNLITDDSVSYILKHQNNDGLFYDSIIEKNNKNQHDYKYISHQITYHAIMVCD
                     MLNIELPHNLVFLKEYKDKAYLKSWLENLNWKNPWLVSNNIMFILYFFIYEQENYELE
                     NEIYIQTIIEWLIKNQSNNTGYWDCNNKSSLHNQMAGAFHFLFFFDYLGINIQHKEEI
                     INSTLLIQEYDGLYSYASGGGSCDDLDGIDILCRLFNSGAEKNKIRKSLLKSYNSLTK
                     NQNYDGGFCWAKRRNFNWKNIIHIIPLRLILKRKNIDAKNSFFMKNKNIIYSLLFKQK
                     LTWCYSGIKDYKIYIDDSDIFSTWFRVLSLTLIEKTLDNKNNIIREKCGIGFFKKC"
     gene            6177..7367
                     /gene="wzy"
     CDS             6177..7367
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ADL32296.1"
                     /translation="MLILTSLILFFLPITFYKSNLMKLFLVIYAYPIFIIFIMCFNYD
                     VGRIIKQIGLNYYNKQDMITSIIPLVISYIFILIISLSYRKTIFSLKTISLGIPVRCI
                     LFFLFLLISFIAYPKAMGISDTIRYNLIFGSWGGVFNVLSIFILFSKSKKYDVINILT
                     YTILIICFLSGERADTIITIILLFLLKKDSNGNITERKINIIKLFLILISFSILASII
                     GAARSNIQIQDISLFITKSLLSVGTVVDVIHVYLSSIWYVEHNGNSFTPLFNIIKSFI
                     PLAGGGGVSSEENITWFLNNYIHNVGGGLFYSPFYIAFSSYGVILFNIIYFLFFTFTF
                     KNKNNYIKYIFIVFYIMQCRIQWYGITYFFTSFKLTLLFLIIIYFLKKFPKKLSNEKN
                     IIHK"
     gene            7342..8511
                     /gene="wemM"
     CDS             7342..8511
                     /gene="wemM"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32297.1"
                     /translation="MKKILFINDNSLKGGSAQVTNDTKKCFDDRGWKSYTFFGSTYFN
                     FNPRNYIFNINAYKKLKTTLEKTTPDVVHIHNYDNLLSPLILLAIYLHKKKNTYCKII
                     MTVHDYHIISPSNSLSYYKGKEKAFFVTPPSFLKLLTSKLDRRSYIHGLARILQWYIY
                     YSLFDLRKVIDTYICPSNFIYNLLPFENKKLIYNPINNLNAFNNNIKKCDSQIMITFA
                     GRLTKDKGIYDFLECICNCDTSSQKLLSINIFGEGELKEKIIRLKEKLKEKNIDIILY
                     GFKDKNYIQEILNKSHYILLPSLCYENAPLSLIEGIFSKCKVITMNYGGMKEIAEKSK
                     NSILLNNITTSEIKKMITTINKQYKNEAGFYSHSQYNKYSLEYYYKEINHTLEKI"
     gene            8546..9658
                     /gene="wemN"
     CDS             8546..9658
                     /gene="wemN"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32298.1"
                     /translation="MKIIAITSNTSWYLYNFRKNTIISLIEAGFKVYTVSPIDSYSNK
                     LKELGAEHINIKIDSASKNPLIDLYTLISFYKIFKQLRCNAVLNFTPKNNIYGTLAAR
                     LNKIKVINNIAGLGTLFVNDTISCKLARNLYRYSQKYADKIFFQNNDDLSLFLEKKYV
                     DKVQVDRLPGSGVDLSRFTLSLSEYKHKFRFLLVARMLYEKGVTFYVEAARILKERYG
                     DKVEFCLLGFVGVNNPSAITHKQMNNWVAEGIINYLGTSDTVENELAQADCIVLPSFY
                     REGVPKTLLEAGAMGKPIITTDNVGCRETVTHGFNGYICQPKSVSSLVDAMDRFINLP
                     YEKKLKMGQNSRQKIETEFDERIVIKKYLDALKEIM"
     gene            9673..10839
                     /gene="ugd"
     CDS             9673..10839
                     /gene="ugd"
                     /note="UDP-Glc-6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="Ugd"
                     /protein_id="ADL32299.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYNLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIHNLDEFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     gene            10865..11875
                     /gene="gla"
     CDS             10865..11875
                     /gene="gla"
                     /note="nucleotide sugar epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gla"
                     /protein_id="ADL32300.1"
                     /translation="MKYLVTGAAGFIGFHLIKKLIQQGETVVGIDNLNDYYDVALKEA
                     RLNLLNQLDNFSFSFIDLADREKIAQLFEIEKFDRVIHLAAQAGVRYSLINPFSYADS
                     NLTGFLTILEGCRHNNVKHLVYASSSSVYGLNDELPFSPHDQANHPVSLYAATKKANE
                     LMAHSYSHLYGIPTTGLRFFTVYGPWGRPDMALFKFTKAIINNQPIDIYNHGEMKRDF
                     TYVEDIVEGVTRIADVIPTAQQDWKVSTGTPADSSAPYKVYNIGNGSPVNLMDYISAL
                     EIHLGKKADKNMLPMQPGDVYTTWADTEDLFKATGYKPQTSVDEGVKQFVDWYKNYYQ
                     VK"
     gene            11879..12382
                     /gene="wemO"
     CDS             11879..12382
                     /gene="wemO"
                     /note="methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WemO"
                     /protein_id="ADL32301.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     gene            complement(12512..13066)
                     /gene="wemP"
     CDS             complement(12512..13066)
                     /gene="wemP"
                     /note="serine acetlyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WemP"
                     /protein_id="ADL32302.1"
                     /translation="MHAQAKVTLAELKDCLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGDHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGEGCRYITKVIADIRRV"
     gene            complement(13069..13902)
                     /gene="cysE"
     CDS             complement(13069..13902)
                     /gene="cysE"
                     /note="serine acetlyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="CysE"
                     /protein_id="ADL32303.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPMLASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     gene            complement(14002..15015)
                     /gene="gpsA"
     CDS             complement(14002..15015)
                     /gene="gpsA"
                     /note="glycerol-3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="GpsA"
                     /protein_id="ADL32304.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
BASE COUNT     5359 a   2401 c   2306 g   5032 t
ORIGIN
        1 gagttattac gtgaaaccgt aaaagcgaat gagctttgga acgatatttt agataatgcg
       61 aaatttgagg cagaacaaag caataaaacc ctaacagtaa ccgccccgcc aggcccatgg
      121 acgatttatt gcaatcctta ttcattagcg agtgcctttg aaaatatcgt tcgcaatgcc
      181 ttgcgttatt cacattctcg tattgaagtg gcttttaccg aacaaaatca gggtatcact
      241 attattgttg atgatgatgg cccaggggtt agcccagaag atcgcgagca tattttccga
      301 cctttttatc gtacggacga agcacgtgat agagaatccg gcggtacagg actaggactg
      361 gcaattgttg aaaccgcaat tagtcaacat cgaggccatg ttaaagctga cgatagccca
      421 ttaggggggt taagagtgga gatttggttg ccgaaaacaa aataaacata agctatctaa
      481 aagccgcctt tagatttaaa atacaaaaat actaaacttt aattaatcgt taaaatgaat
      541 aaatacttaa aaattatcag aaataaatta cgttataagt actgtgatgt ttttccaaaa
      601 ttgataacaa aatcaatata taaagaaaga atgaataaaa aattagattt gagacatccg
      661 caaactttca atgaaaaatt gcaatggcta aaactaaatt tatatagaga taacccgctg
      721 gtaacacaat gtgcagataa atatgcggct agagaatatg ttaaagaatg tggctgtcaa
      781 gagatactca atgaaatcca tcaggtatgg gaagaaccac atgaaattaa tttttccgaa
      841 ttaccaaata aatttgtact aaaatgcaac catggagcag gatacaatat tatttgtaga
      901 gataaaaata gtatatctcc tgataaaata aaacaaaaat tatcaacttg gttaaatgaa
      961 gattattggc gtttatctgt agaatttgtt tataaagatg tacctaaaaa aataatttgt
     1021 gaaaaattca ttgaaacaaa aaataatgaa ttgccatacg attacaaatt attttgtttc
     1081 catggtaaac cactatttgt aatgatatgc acagaaaggg aatctcacca tcccaaattc
     1141 tattttgtta ataaagaatg ggagctacta ccctatggta ttgattataa aaatagaaat
     1201 gtttcaactg tactagagaa acctcaaaat tatcaaaaac tatttgaata tgcagaaaag
     1261 ctgtctaagc catttccttt tgttagagta gatttatatt taaacaatgg tattatccat
     1321 tttggagaat taacatttat accatcagca ggaatggata aagaactaaa taatgaagaa
     1381 gttaaaaacg ttgatatgat ttttggcaac tatcttaagt taaactaagg ggaatcccct
     1441 tagtttatgg acttaattat ttttctatta attaaataaa taaatcgagc tttcaacttc
     1501 atataaggat aataaaaact tttcttctca caacgcctaa ataaagaaac tttttttctg
     1561 atgatggcaa ccttaagctc cttataaaag taaggttttg attgtatatt tttaattctt
     1621 attttttcca acaaagaatt acattttttt atattatcta atgtgagata ttcagaggaa
     1681 aaattcaaat catataaaac tttctcattt gcattaatac taaatatctc taatgcgcta
     1741 atttgtgttt ttatagcaaa tgaagtttgt gtattaaagt atttttttct atttttttct
     1801 ggatgggttc gatattttaa taaaggttct tctaaattag caaaaatatc ttttttaccc
     1861 aactttaata atttaaccca taacccataa tcctgagcat atttgtaatc ttctggatat
     1921 gtgatttgat tatctccgat aaaactcgct ctaaacatca ccgttggatg ataaaaattt
     1981 gttccaaaaa ttaatcccgc agcaatggca ttatgtgcag ttggtggaac cataaactta
     2041 ttatggtctt cataaatttc cattgatgta ccacaaacca tgatattttt atgcgcctcc
     2101 ataaactcta attgtttttg aagtcggata ggatatgcat agtcatcagc atccatacga
     2161 gcaatatact caccagaaga gagtaaaaaa cctttattca aagacttagc taatccaata
     2221 ttagattcat tagtatatac tttaactcga ctatcttgct tttcgtatcg tctaagtata
     2281 tctacagaca catcagtaga accatcatca ataatgataa attcaagatc tttaactgtt
     2341 tgattaagaa tagaatttat cgcttcttct aagtgtactg catcattata tactgacatt
     2401 aatacactaa ctttgggatg atttttcatt tttaattaaa tgttatttcc tatagtcgac
     2461 attgcatata ctataaatta ataaataaaa ttagtgaata tcaataaaca tttaaagtag
     2521 ttgaaataat tataatcatc gagcatcaat agctatgaca taacataaaa taacaatgtt
     2581 tacactgagt tcttacccct tctacagaaa acatttaaac tatataaatc agcaagatat
     2641 catagttaca aagaacgcct actaacaacc aatttaaata aaaaataaaa ttaatatatt
     2701 agcaaactct accatttatt aataaattgg tagcatattc ttcttatacc tatcttttat
     2761 acataattca gaatgatctt gccaaatcgg ccgatattcc gatacttata tatacctaat
     2821 caatgttaga atacccatca attacttgac ccgctggttt gaagagtcta atctcagtgg
     2881 ggtttgtgta cagataaagt atacttataa aataaaactc ttatattaca gatagataag
     2941 ggttttaaaa gagaaatttt ggcgctctgg gggccgtaaa cctagtgcgg tagctttgat
     3001 tataatataa taaacaatag attaggtttt tactaaaaat gcaaaatata gaaagtacaa
     3061 aagtatctat aataatctca gcttataatg cagaaaaata tattcatgaa tcattagaat
     3121 caataataaa tcaaactcat aaaaacctag aaataatagt cattaatgat ggttctacag
     3181 acaatacaaa gcatatatgc actgaattcc aaaaaagaga tccccgaata atattcattg
     3241 ataaagaaaa tgaaggatta acaaaatctc tcaataaagg aatacattta gcaactggaa
     3301 catggattgc tagacaagat gcagatgaca ttagcttaga aaatagaata gaagaacaac
     3361 taaagttttg tataaaaaat aaaatagatt tctgtacatc acaagcaata caattttccg
     3421 ctcaaaataa tcttagatta atacctaaaa ttcgttcaag tatcgcgttt aagaagaaaa
     3481 ttttaaaata tggtaaccct catatacatg gaactttctt ttttagaaaa aaaattatag
     3541 aaaaaataaa ttacgatgaa gagataaagt atgctcagga ttttaatttt attctggcag
     3601 ttaaacatgc aaaattcaat atggctttac taaaaacacc actatataaa cttagaattc
     3661 acaataattc tatttcctca aataaaaaga aagaacaaat tgaggcagta aaaacatcgc
     3721 ttgaaaaata tagatatagc acacactata taatagaaga aaataatggt ttaaagaaac
     3781 aagcactaag aattatgaag caatgtgagt ctataattta atgaaaaaat taatatatga
     3841 tattatttat aaatttagtc agggtggtat taaacactta ttacaactta ttattttatt
     3901 ttatggttcc tcacacctag gagctgataa ttttggtgaa tatatatata ttacaacaat
     3961 aatagttaca ttaagcttat ttttagattt tggcttatca accacactaa tgaaagaaat
     4021 agcagaaaat aaaggaaacg aagaaaagat atatagatca attattcctt atttctttgt
     4081 attgacttca ctatctggga tcattttttt tgttttatca aatttagaat ttaatcttaa
     4141 aatactatta ggaataacat tatctataat gccaatttgt tctatttttg atggtattca
     4201 tcgaggaaaa ctagaattta aaataaatgc aaaatataat tttttatcag ctttactttg
     4261 tctattttta atatttcctc ttgtgtacta tttaaaaata gcaggattgc tacttttaaa
     4321 tcttattttt tattcatcat tgttactaca atatatttac aattgcagaa aaaaaatatt
     4381 ttctaaagtt tcttttgata aaaaatattt attaaaagtt acatcttact ctataattat
     4441 cggcattggg tttgttggcc aatttttata cacaaaggca gatataataa tattaggtaa
     4501 atataattat ttatctgaaa ttactaccta tgaaatattt gatcgtttat tgatgtattg
     4561 cattttacct tttggattaa tagctcaagt tttagccccc agagttatta atactaatat
     4621 tgataaaata aaaaaattaa ctaatatttc cttttgcata agtattatgt taagtattac
     4681 tttatattta ttatcatcct atattttaaa aaataactat aatactcagt tattaggtat
     4741 tccttattct acttattcat ctattattcc cttactcata gcttgtgccc caataaggat
     4801 atttgccata ttacaaactc aagccttaat cattccttta gggtttgcaa aaatagtagc
     4861 gtcaacaacc ttattttttg gcattattaa tgttatatta gatttttcat tgttatattt
     4921 atatggtttt ataggtattt tttatagtac tttaattgtt gttagtttaa atacattatc
     4981 tcagtatatt ttatttggaa ataaatttaa atggaaaaaa actggtctaa gcaagcctta
     5041 atttttataa actcattaaa aaatgataat ggttcatatt ctttttctaa aaaaagtagc
     5101 tgtgcaagct gtttatcaac gagttttgca ttacttgcat tagaagtatt gcaacaaaaa
     5161 aatttaataa cagatgatag tgtatcatac attctaaaac atcaaaataa tgatggctta
     5221 ttctatgatt ctatcattga aaaaaataat aaaaaccaac atgattataa atatataagc
     5281 caccaaatca cttaccatgc aattatggta tgtgacatgt taaatataga actacctcat
     5341 aatttagttt ttctaaaaga atacaaagat aaagcatatt taaaatcttg gttagaaaat
     5401 ttgaattgga aaaatccatg gttagtttct aacaatatta tgtttattct ctattttttc
     5461 atatatgaac aagagaatta tgagctagaa aatgaaattt atatacaaac gattatagaa
     5521 tggctcataa agaaccaatc taataacact ggatattggg attgtaataa taaatctagt
     5581 ttgcataacc aaatggctgg agcttttcac tttttattct tttttgatta tttgggaatt
     5641 aatattcagc ataaggaaga aataattaat agcactttat taattcaaga atatgacggg
     5701 ttatattcat atgcctctgg tggaggttca tgtgatgatt tagatggtat agatatatta
     5761 tgtagattat ttaatagcgg agcagaaaaa aataaaataa gaaaatcgct attaaagtct
     5821 tataactcac tcactaaaaa tcaaaattat gatggtggtt tttgttgggc taaaagacgt
     5881 aactttaact ggaaaaacat tattcatata ataccactac gcttgatttt aaaaagaaaa
     5941 aacattgatg caaaaaatag tttttttatg aaaaataaaa atataattta ttcccttcta
     6001 tttaagcaaa aactaacatg gtgctatagt gggattaaag attataaaat atatattgat
     6061 gatagcgaca ttttttcaac atggtttagg gttttatcct taactttaat agaaaaaaca
     6121 cttgataaca aaaataatat tattagagaa aagtgtggta taggtttttt taaaaaatgt
     6181 taatattaac ttctttaatt ttattttttt tacctattac tttttataaa agtaatttaa
     6241 tgaaattatt cctagttata tatgcttatc ctattttcat tatatttatt atgtgtttta
     6301 actacgatgt aggtaggata attaaacaga taggattaaa ttactataat aaacaggata
     6361 tgattacttc aatcatccct ctagtcatta gttatatttt tatcctaatt atttcactaa
     6421 gttatagaaa aaccattttt agcctaaaaa ctatttcttt agggattcca gttcgctgta
     6481 ttttattttt tttgttttta ttaatttctt ttattgcata ccctaaagca atgggaataa
     6541 gcgatacgat acgatataat ttgatattcg gttcatgggg aggtgtattt aatgtattga
     6601 gtatctttat tttattcagt aaatccaaaa aatatgatgt aataaatatt cttacttata
     6661 ctatattaat aatatgcttc ctttctggtg aaagagcaga tacaataatc actattattt
     6721 tgttattttt attgaaaaaa gatagtaatg gtaatataac agaaagaaaa attaatataa
     6781 taaagctttt ccttatatta atttctttct ctatcttagc tagtattatt ggtgctgcta
     6841 ggtctaatat tcaaattcaa gatatctcat tatttataac aaaatcccta ttatctgtag
     6901 gtactgtagt tgatgtaatt catgtatatc tttcctctat ttggtatgtt gagcataatg
     6961 gaaactcatt cactccatta tttaacataa ttaaatcttt tatacctttg gctggaggag
     7021 gaggagttag ttcagaagaa aacattactt ggttcttaaa taattatata cacaatgtcg
     7081 gtggtggact tttttattct ccattttata ttgcattctc tagctatggt gttattttat
     7141 ttaatataat atattttcta ttctttacat ttacatttaa aaataaaaat aattatataa
     7201 aatacatatt tattgttttt tacattatgc aatgtcgtat acaatggtat ggaataacat
     7261 atttttttac atcatttaaa ctaacattac tatttctcat aatcatctat ttcttaaaaa
     7321 aattccctaa aaagttaagc aatgaaaaaa atattattca taaatgataa tagtctcaaa
     7381 ggcggtagcg cccaagtgac taatgatact aaaaaatgtt tcgatgatag aggctggaaa
     7441 tcatatactt tctttggtag tacctatttt aatttcaatc ctagaaatta tatttttaat
     7501 ataaatgcat ataaaaaatt aaaaacaact ttagaaaaaa caaccccaga tgttgtacat
     7561 atacataatt atgacaactt acttagtcct ttaattttat tagctatata tctacacaag
     7621 aagaaaaata catattgtaa aataattatg actgttcatg attatcatat tatatcacca
     7681 agcaattctt tgagttacta taaaggaaaa gaaaaagctt tttttgtaac tccaccttct
     7741 tttttaaaat tgttaacatc taaactggat agacgttcct atattcatgg cttagctaga
     7801 attcttcaat ggtatattta ttactcttta tttgatttaa gaaaagttat agatacttat
     7861 atatgtccta gtaatttcat ttataacttg ttgccatttg aaaataaaaa acttatttat
     7921 aatccaatta ataatttaaa tgcattcaac aataatataa aaaaatgtga ctcacagatt
     7981 atgattacct ttgctggtag attgacaaag gataaaggta tttatgattt tcttgaatgc
     8041 atttgtaatt gtgatacttc ttctcagaaa ttactgagta taaatatatt cggcgaagga
     8101 gaattaaaag aaaaaattat tagattaaaa gaaaaattaa aagaaaaaaa cattgatata
     8161 atattatatg ggtttaaaga taaaaattat atacaagaaa tattaaacaa atctcactac
     8221 atattattac cttcactatg ctatgaaaac gctcccctat cattaattga aggtattttt
     8281 tctaaatgta aagttatcac aatgaactat ggtggaatga aagaaatagc agaaaaatca
     8341 aaaaactcta ttttattaaa taatattact actagtgaaa ttaagaagat gataacgact
     8401 ataaataagc agtataaaaa tgaagctggt ttttactctc acagtcaata taataaatat
     8461 tcactagaat attattataa agaaataaat catactttag aaaaaatatg attctatact
     8521 ggaattttaa gtaaaagact aaaacatgaa aattatagcc attacatcaa acaccagttg
     8581 gtatttatat aactttagaa aaaatactat aatatcttta atagaagctg gatttaaagt
     8641 ttatactgtt tcacctattg atagttactc taacaaacta aaagaattag gtgcagaaca
     8701 tataaatatc aaaattgatt cagccagtaa aaatcctttg attgaccttt atacattgat
     8761 ttcattttat aaaatattta aacaacttag atgtaatgct gtacttaatt tcactcctaa
     8821 aaataatatt tatggaacat tagcagcaag attaaacaaa ataaaagtca tcaataatat
     8881 tgcaggttta ggtacattat ttgtaaatga tactattagt tgtaaactag caagaaattt
     8941 atatcgctat agccaaaaat atgctgataa aatatttttt caaaataacg atgacctttc
     9001 attattttta gaaaaaaaat atgtcgataa agtacaagtt gatagattgc ctggttcagg
     9061 tgttgattta tctcgcttta cattgtcatt gtcagaatat aaacataaat ttagatttct
     9121 attagtggca agaatgcttt atgaaaaagg ggttactttt tatgttgaag ctgcacgtat
     9181 cttaaaagaa agatatggcg ataaagttga attttgtctt ttaggatttg ttggtgtaaa
     9241 taatccttca gcaatcacac ataaacaaat gaataactgg gtagcagagg gaattattaa
     9301 ttatttaggt acatctgata ccgtagaaaa tgaattagcc caagcagatt gtatagttct
     9361 accttctttt taccgagaag gagtgccaaa aacattacta gaagctggag ctatgggaaa
     9421 acccataatc actactgata atgttggttg tcgagaaaca gttactcatg gttttaatgg
     9481 atatatatgt caacctaagt ctgtatcaag cttagttgat gccatggata gatttattaa
     9541 cttgccctat gaaaaaaaac taaaaatggg tcaaaatagt aggcaaaaaa tcgaaactga
     9601 atttgatgaa agaatagtca taaaaaaata tttagatgcg ttaaaagaaa ttatgtaact
     9661 ttaggataaa aaatgaaaat caccatctca ggaaccggct atgtcggtct atctaacggg
     9721 atactccttt cccagcacaa caaagttgtt gcattagata tcaataaaga aaaagtcgaa
     9781 ctattaaaca aaaaaatctc tcctatctct gataaagaaa ttgaagagtt tttagctact
     9841 aaagaactcg actttacagc aactttagat aaagaattcg cttataaaga tgctgaattt
     9901 gttatcattg ccactcctac cgattatgat cctaaaacta actacttcaa cacctcttct
     9961 gtggagtcgg ttattcgtga tgttttagaa tataatccaa acaccactat gattataaaa
    10021 tcaaccattc cagttggttt tactaaacag atgagagaaa aatatcatac cgataatatt
    10081 atcttctctc ctgagttctt acgtgaaggt cgtgcccttt acgataacct ttatccatct
    10141 cgtattgtga ttggtgagca atctgcaaga gcagagaaat ttgctgagct tttacaacaa
    10201 ggggcattaa aaaaagatat tgatgttctt tttactgatt ctacagaagc agaagccatt
    10261 aagttatttg ctaatactta tctcgcactg cgtgttgctt attttaatga actcgatagc
    10321 tacgcccaag cttacaattt aaatgcaaga caaattattg aaggtgtctg tttagatccg
    10381 cgtattggta atcactataa caatccatca tttggttatg gtggctactg cttaccaaaa
    10441 gatacgaaac aactgttagc aaattacgaa tccgtgccta ataatattat tggcgccatt
    10501 gtggaagcta accgcacccg taaagacttt attgctgatt ctattatcgc taaatcaccg
    10561 aagattgttg gtgtttaccg tttaattatg aaatcaggct ctgataattt ccgctcctct
    10621 tcaattcaag gcattatgaa gcgtattaaa gcaaaaggca ttgaagtagt gatttatgag
    10681 ccagaaatga aagaagagac tttctttaac tcaaaattaa ttcataattt agatgagttt
    10741 aaagccatgt ctgatgtcat tattaccaat agaatggctt cagcactaaa cgatgttgaa
    10801 gaaaaagttt atactcgaga cctctttggt aacgattaat taatagaaac ggattaatta
    10861 cgtaatgaaa tatttagtca caggtgctgc tggatttatt ggttttcatc taataaaaaa
    10921 actgatccag cagggtgaaa ccgttgttgg tattgataac cttaatgatt attatgatgt
    10981 tgctttaaaa gaagctagac ttaatctttt aaatcaacta gataatttct ctttttcttt
    11041 tattgattta gctgatagag aaaaaattgc tcaattattt gagattgaga aatttgatag
    11101 ggttattcat ttagctgcac aagctggtgt tagatattca ctaattaatc cgttttctta
    11161 cgctgatagt aatttaactg gttttctaac tattttagaa gggtgccgac acaataacgt
    11221 taagcatctt gtttatgcat catcaagttc agtctatggc ttaaatgatg aattgccatt
    11281 ttctcctcat gatcaggcta atcatcctgt ttctttatat gcagccacta aaaaagctaa
    11341 tgaattaatg gcgcatagct attctcattt atatggtatt ccgactactg gattacgttt
    11401 tttcacagtc tatggccctt ggggacgccc cgatatggca ttatttaaat ttactaaagc
    11461 cattattaac aatcagccta tagatatcta taaccatggt gaaatgaaac gtgatttcac
    11521 ttatgttgaa gatattgtag aaggtgttac acgtattgct gatgtgatcc ctacagccca
    11581 gcaagattgg aaagtaagta caggtactcc agcagatagt tctgcaccct ataaggttta
    11641 taatattggt aatggatctc cggtaaattt aatggattac attagtgcat tagaaataca
    11701 tttaggtaaa aaagcagata aaaatatgtt accaatgcaa cccggtgatg tgtacaccac
    11761 atgggcagat actgaggatt tatttaaagc aactggctat aaacctcaaa caagcgttga
    11821 tgaaggtgta aaacagttcg ttgattggta taaaaactat tatcaagtaa aataaataat
    11881 gctcaatatc gtcttatttg aacccgaaat tccaccgaat acgggtaata ttatccgcct
    11941 gtgtgccaac acaggctttc atcttcattt gatccaacca ttgggtttta cttgggatga
    12001 taaacggcta cgtcgtgcag ggcttgatta tcgtgagttt gctgatatca aacagcatca
    12061 cgattattat gcttttttag aaagtgaagg attaagccct gataatgcac aatccccgac
    12121 aggtgcgcgg ttgtttgcat taaccactaa aggtactccc gcccatagta atgtaagtta
    12181 tcaagatggg gattatctgc tatttggccc tgagacacga ggtttaccac cttatgtgtt
    12241 ggataatatg ccaacacaac aaaaaatccg tgtgccaatg ttagcggata gccgcagtat
    12301 gaatctttct aatacggttg ccgtggttgt ttttgaggca tggcgccaat taggctatcc
    12361 gggtgcgtta ttaagagatt gatttagtgg ttagtttggt taattaaagt tattttaatt
    12421 catacttatt acttgatttt atctttattt ataaactaaa aaaggtgcta atatttattc
    12481 agcacctttt ttataaacct ttgttcaagc attatacccg cctgatatcg gcaatcactt
    12541 tcgtgatata ccgacaccct tcccccacat cctttaatac taatgcatgg gcgccaataa
    12601 tcgcattatc accaatatta atctcaccac caataatgga tgaattacag ccaatataaa
    12661 catgatctcc tacttggata ttggcttttc cccctttctc ttttacgcca atagtcaccc
    12721 cttgcataat agtgacatat tcccccagtt ggcagtaatt agtcagtaca atgccaatcg
    12781 ggtgtggtaa agaaaggcct ttaccaatac gagtttttaa cgagatatca ttacagtact
    12841 tacgattatt gctagcaccg atccgaatac ctaatttatg taaaaagcga tggggagaga
    12901 aataaaaata ctgtgctacc cgccaccaaa ataggtaacg ggaacgctgg cagaaaaata
    12961 ctcgatgaaa tagtcttaac caactaaagg gttttttatc accaatatat tcagcctgta
    13021 agcaatcttt tagttcagca agggtaacct tagcttgagc atgcatagtt aaataccatc
    13081 accacactca aagccgggta caacaccatt aaagtgttga tccatatcga atgacggttt
    13141 ttcactatca ggacgaccga caatgcgggc aggcaccccg gccaccgtag tatgagctgg
    13201 tacttcatgt aatactaccg agccagcacc aattttagca ccacgaccaa tttcgatatt
    13261 accaaggatt ttagctcctg cgccaatcat taccccttca cgcactttcg ggtgacgatc
    13321 acctcctgtt ttacctgttc cccctaaggt cacagattgt aaaatagaga catcattttc
    13381 aaccaccgcc gtttcaccaa tcacaatacc tgtcgcatgg tcgagcataa taccgtgtcc
    13441 aatacgtgcc gctgggtgga tatccacccc aaaggtgact gatatttggt tttgtagata
    13501 aatagctagc gcttggcgat tttgttgcca tagccaatgg gcaatacggt aggcttgtag
    13561 tgcatgaaac ccttttaaat agagcagtgg ggtagaatat ttatccaccg cgggatcacg
    13621 taggcgtacc gcggagagat cacaagcggc cgactcaatc attgagctat cgtgactata
    13681 agcctcttca acaatttcgc gaactgcaat ggctggcata gtctgagttg ccagcttatt
    13741 ggccagcata tagcttaatg cactacctaa attttcgtgt ttgagaagtg tcgcatgaaa
    13801 gaaactggct aacattggct cacaatctgc caatgcacgc gcttcgcttt taatatgatc
    13861 ccagactctt gttagttctt ctagcgacat actcactctc attttttaca cgaaagccta
    13921 agtaaatact taggctttga attttcaaac aaatggatag ttaaaacaga taccctaaga
    13981 atattattta aaataaactg actacaggct actgtctatc tcatctttgg ttgctcgccc
    14041 taataaagcc aatgcagcct cttttacatc tttatgttga tataaaattt ggtaaatctg
    14101 ttcggtgatt ggcatttcta cacccacttg ttcggctaat gcgcgaactt ctttggtgtt
    14161 acgataacct tcgactacct ggccaatttt ctcttgggct gtatcaacat ctaaaccttg
    14221 acctagcatc ataccaaagc gacgattacg tgattgattg tctgtacagg ttaaaaccaa
    14281 atcacccaaa ccagccatgc ccataaaagt tgccgcatct gcacctaagg ctttacctaa
    14341 acggctcatt tcggctagac ctcgggtaat cagtgcggta cgcgcattag cgccaaatcc
    14401 cataccatca gacatacccg caccaatagc aatcacgttt ttaacagcgc cacccagttg
    14461 cacaccgata aaatcagggt ttttatagac tcggaaactt ttaccacaat gaaatagctg
    14521 ttgtaactgc tctaaaaaga gattatccgt cgaggccact gcaatcgcgg tgggcaaacc
    14581 cgcggcaagc tctttagcaa aagtagggcc agataatact gcgagcggga tttcattacc
    14641 taatacttca cgggcaacat cttgtaataa gcgaccagta tgtgcttcaa gaccttttgt
    14701 cgcccaaaca acacgtgcat cctgacgtaa aaagggtttg atttgttgca gtacttcacc
    14761 aaacacatgg cttgggatca ccacaagaat attacggctc gcttcaatcg ctttttgcaa
    14821 agaagcttcc atatataaac tatcaggaaa ggaaacatcg ggcagaaagg cttgattaca
    14881 gcgcgcttgt tctaatgccg caacgtgctt gggatcatgc ccccacagca caacatcgtg
    14941 cccattacgc gctaaggtaa tcgctaaagc ggtgccgtat gaaccggcac cgataactgt
    15001 catagaagcg ttcattaagc ttcttcaact cctgagtttg ctgcatcctg ttgctgttgt
    15061 tgctgtaaat agttcataaa caacgcatca aagttaac
//
