LOCUS       KY710693               14896 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 26/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710693
VERSION     KY710693.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 14896)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14896)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14896
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 26/57"
                     /serotype="O13"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     gene            1..1258
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..14896
                     /note="O antigen gene cluster"
     CDS             1636..2640
                     /note="orf02"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf02"
                     /product="hypothetical protein"
                     /protein_id="AXY99444.1"
                     /translation="MKKITLGLICYPGFEIDKMKQACIDNDVNYIEIDFLSTDWLNQC
                     RNPTIDGFIIRPPCTLQEHKNIFDERVYFINNILKKMTYPSFKELYIYENKRNMALYL
                     EGIGAPHPKTKVFLDKKSALQRTPNFPVVMKSNIGASGSAVKIIKNNYQYKKMIKKIF
                     GYIHPEFSLGWIPFVKYKNIPLPRIGRSQKHYAILQEYLDIKWEWRMIRIGNSYLGHQ
                     KLLGDNGYASGSELVGWEEPPKELLLLLHSVTEKLNSRCMVLDIFETKEGKFFINEMQ
                     TIIGAYRPYQMKINNKPGRFILKENNFIFEEGIHFQNACWNPRVEDFKKILLEEKYYA
                     "
     CDS             2633..3745
                     /note="orf03"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf03"
                     /product="hypothetical protein"
                     /protein_id="AXY99445.1"
                     /translation="MHNLIAIFGTSAKKNENRIPLHPSLFHTIPQEVKKHLIFEKGYG
                     RRFNINDDYFIKNFAGVAERKDLFLTSNVWVLPKPEQEDYKYFSSGKILFGWLHCVQG
                     YEITQAAIENNMTLISWESMYGGNDNTHIFNRNNELAGYAAVQHMLMLQGINGYFGKK
                     LKAAVLGFGATGRGSINSLKSLGINDITVFSNRPTFLIKSPIESIDYRKITHNNNDVY
                     LEDDKKNIVEPIEVLNKFDIIINCILQNPVSPITFIHNKEINKIDKRTIIIDISCDKG
                     MGFEFARPTNFSKPFISINENINYYCIDNTPSIYWESASYEITKSLTPFLELFINNSW
                     KDDIIIKNAIEIENGIIKNKKIIDFQKRNKDYPYKI"
     CDS             3748..4959
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99446.1"
                     /translation="MNNKKEIINNLLSFAIIDALNLLIPIITMPILTRALGSSSYGDY
                     MLLITILTFGHTIIDYGVQFTGVRDLSRNRNNQSIYSSIYLENQVIRFIFLFLYIIIT
                     IFFLIISKKIELLYYFLYGGVFYLVGYFLASPWFFQGMGKTKILMIVTFIFKLFNLVG
                     IIFLVKNKEDIYIAVFLSTWTMFISGSILFFISKNKIIFKKFSFEDLILRLKNSFNVF
                     IGILAPNLYNSVPTIYLGTIAKPSYFAFYIVAARLCDIIVVIQNIISKSIYPVISRSK
                     KNHIKVILLTNISLATPIIIFISLFGSDLLSLFLGNEYSNSQSYLLIFSFGILFIGIA
                     NSFSQGFFLPKNYDTIYRKVSLRVSVLSAVICYILIVKYNLIGGAIGITMARILFSLD
                     YAVTYFLMKKN"
     CDS             4961..6007
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99447.1"
                     /translation="MNIYIYIWVFIVINILSPTKIKNNNFVFFSFFIFLFLFIGLRYE
                     IGGDWNNYSRMYSTISQLSLTESLKFTDPAYGLLNWIAFKFSLGLVFINIICSFIFIS
                     GLYLYSKDKYGRWLIVLIAYPYLILAVSMGYTRQAAAFGFFLLSIIALSQNKPIKFIF
                     LIFLGTLFHKTLIIFIFLLPFFIKDKKKKRNVFLSILFISPFLFLAFYEKIVNQLYFY
                     FENELHSDGGLLRLLINIIPSILFFIFYNRWKKSYSEDTQLILILSLLSFILLAFQFF
                     ASTISDRIGLYLIILQLIIYPKILYLIKDKVIHSVFSLSIIAIYSGMLFVWLNYSPWA
                     QCCWIPYKNYLFNY"
     CDS             6014..7144
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99448.1"
                     /translation="MIKKPKIIHIITGLNNGGAEGVLYRLCKYDLANDHVVISLTTDG
                     KYGSMLRDINVNVICLHLKSFSSTLPSLIKLYKIIKKEKPYIVQTWMYHADLIGGIIA
                     KLAGVKKIYWNIRQTNLIKGKSKKTTILIAKICSKFSFFIPCKIICCASEAKKTHIDL
                     GYKGKDMVIIPNGFDFSQYKKSHNDEIKKQLGIPQNSICIGMVARFDPQKNHLLLLDA
                     FTRIYKNKNIKLILIGSNITNNNKILVEYIKNKNIEKETILLGERKDIPDMMSLLDIH
                     ILSSSFGEGFPNVLVEAMASGTPCIATDVGDSRLIISEYGWIIPPNNLNKLISALNSA
                     LSIKELDNKKWEDICVACKKHVISNYDIEKMVDAYNSTWFEK"
     CDS             7151..8287
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99449.1"
                     /translation="MIKIAHIQLLPLLSGAQKVSLEELQRLDPKIYDRYLICKEPGPL
                     SFEAEKNNIKCIYIKNLVREISLKNDILALIKLIRIIKKLKFDIIHTHSSKPGVLGRI
                     AAFISRVPLIIHTVHGYSFPAAKNKLQYYIYLIMEYIGAKSGNLLICLHEKDKNIAIK
                     KLRIKDKNIFVLANGVNVEKYKPNLNRSLIRKRLNFNNDDIIIGMTGRLWEQKNPMLL
                     LYAFDKIINKDNLKLLFIGDGELNEKMIDFINEKKISNNVIMLGWREDTDQILNSLDI
                     FVLPSNWEGMPLAILEAKSCGLPCIVSNISGNNNLIDNEKTGLLFEPNNSDDLKIKIE
                     LLINNKETRELFGNANRVDILTNYNIDDRIEKIDKLYQSFLNKH"
     CDS             8299..9465
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99450.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYNLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIHNLDEFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     CDS             9491..10501
                     /note="NAD-dependent epimerase/dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gla"
                     /product="gla"
                     /protein_id="AXY99451.1"
                     /translation="MKYLVTGAAGFIGFHLIKKLIQQGETVVGIDNLNDYYDVALKEA
                     RLNLLNQLDNFSFSFIDLADREKIAQLFEAEKFDRVIHLAAQAGVRYSLINPFSYADS
                     NLTGFLTILEGCRHNNVKHLVYASSSSVYGLNDELPFSPHDQANHPVSLYAATKKANE
                     LMAHSYSHLYGIPTTGLRFFTVYGPWGRPDMALFKFTKAIINNQPIDIYNHGEMKRDF
                     TYVEDIVEGVTRIADVIPTAQQDWKVSTGTPADSSAPYKVYNIGNGSPVNLMDYISAL
                     EIHLGKKADKNMLPMQPGDVYTTWADTEDLFKATGYKPQTSVDEGVKQFVDWYKNYYQ
                     VK"
     CDS             10505..11008
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXY99452.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(11146..12111)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99453.1"
                     /translation="MPKITVLMSVYNDEKYLQESIDSILNQTFKEFEFLIINDGSTDN
                     SLSILKENAKKDPRIKLIINEQNIGLIASLNKGLQLASCDLVARMDSDDIAVSTRLEA
                     QYQFMLQHPEVSVCGGAFIYQEEPNRINTYPETHSDIVISSLYDCSLAHPTIIYRKQD
                     ILSVGGYDKADLYAEDYGLWVNLIIAGKFKLHNLPVPLLYYRTHPNVERSQYKAIQKD
                     TAERVGRKLLMYFGINVNKEDYSLLLDNDKKTVEQVVRCKRLLEKINQQFNAKEYRKE
                     LIQKNEFKKKLLERKVLVTDFSSFLIKISARWYFLIRKITKKAKK"
     CDS             complement(12133..12690)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemH"
                     /product="wemH"
                     /protein_id="AXY99454.1"
                     /translation="MHAQAKVTLAELKECLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGDHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGEGYRYINKMNFEINKYC"
     CDS             complement(12693..13526)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99455.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(13626..14639)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99456.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
     gene            complement(14639..14896)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5095 a   2424 c   2462 g   4915 t
ORIGIN
        1 gtgataccgt caaggtgtta tgctagagca acatatagaa gccgaactag cgcaagatcc
       61 ggctaacgat ctgctatggt ggcggcgatt aatccgcgcc attgataaat gggcgccacc
      121 tggtcaacgt cttattattg tcaccagtga aggtcgtatt attggtgctc aacgtaatga
      181 gatacaagtt gtcagaaact ttatggggca gtctgataac gcagaccatc cgaaaaagaa
      241 aaaatacggt cgctctgaga tgttaggccc cttttccatt agagatggtg aagatcacta
      301 ccaactttac ttagtgcgcc catcaagtag cccgcaatct gattttatca atttattatt
      361 tgataaaccg cttttattac tgatattcac tatgcttatc agtacaccgc tactggtttg
      421 gctctcttgg agcttggcaa aaccggcccg aaaactcaaa aatgcggcgg atgatgtggc
      481 aaaaggtaat ctgcgccctc accctgaact ggaaaccggc ccgcaagaat ttttagcggc
      541 aggcaccagt tttaatcaga tgatcagcgc cttagaacgc atggttgaag cgcaacagcg
      601 attaatttct gatatctccc atgaattgcg caccccgtta actcgtttac agcttgccag
      661 tgcattacta cgtcgtcgta gcggtgaaag taaagagcta gagcgtattg aaacagaaac
      721 acagcggcta gatggcatga tcaatgactt attagtgctt tctcgcaacc agtataaaaa
      781 tgagttatta cgtgaaaccg taaaagcgaa tgagctttgg aacgatattt tagataatgc
      841 aaaatttgag gctgaacaga gcaataaaac cttaacagta accgccccgc caggcacatg
      901 gacaatttat tgcaatcctt attcattagc gagtgccttt gaaaatatcg ttcgcaatgc
      961 cttgcgttat tcacattctc gtattgaggt ggcctttacc gaacaaaatc agggtattac
     1021 tattattgtt gatgatgatg gcccaggggt tagcccagaa gatcgcgagc atattttccg
     1081 acctttttat cgtacggacg aagcacgtga tagagaatcc ggcggtacag ggctgggact
     1141 ggcaatagtt gaaaccgcaa ttagtcaaca tcgaggccat gtaaaagccg atgatagccc
     1201 attaggggga ttaagagtgg agatttggtt gcctaggggg agtgtggtaa ataaatcaca
     1261 acacgcttaa ttcagtaata aaattactat attaaattat tctgccatct attgaagaat
     1321 aggtggcata ttcttttcat tcccatcttt atagatattt cagaatgatc ttgctaattc
     1381 agtcgatatt ccgatattta tatataccta atcaatgtta gaatacccat caattacttg
     1441 acccgcgggt ttgaagagtc taatctcaga gcggttttgc gtactgttaa agtatactta
     1501 tataataaaa ctcttatatt acagatagat aagagatcgt cgagaaaatt tttggcgctc
     1561 tgggggccgt aaacccagtg cggtagcttt aatgtcacat aacccatgtt tcatcattaa
     1621 aaaacaaaat atattatgaa aaaaatcaca ttaggtctaa tttgttatcc tgggtttgaa
     1681 atagataaaa tgaagcaagc atgtatagat aatgatgtca attatataga aatagatttc
     1741 ttatctactg actggcttaa tcaatgtaga aacccaacaa ttgacggttt tattattagg
     1801 cctccttgta ccttacaaga acataaaaat atatttgatg aaagggtcta tttcattaat
     1861 aatatattaa aaaaaatgac ttatccttca tttaaagaac tttatatata tgaaaacaaa
     1921 agaaatatgg ctctatatct agaggggata ggtgctccac accctaaaac taaagtattc
     1981 ttagataaaa aatcagcatt acaaagaact cctaattttc cagttgtaat gaaaagtaat
     2041 ataggtgcta gtggaagtgc agtaaaaatt ataaaaaata attatcaata taaaaaaatg
     2101 ataaaaaaaa ttttcggata tattcatcct gagtttagtt tagggtggat accttttgtc
     2161 aaatataaaa atattccctt acctagaatt ggaagaagtc aaaagcatta cgctatttta
     2221 caagaatacc tagatataaa atgggaatgg cgaatgatca ggattggtaa tagctactta
     2281 ggtcatcaaa aattattagg tgacaatggt tatgccagcg gttctgaatt agttggatgg
     2341 gaagaaccac caaaagaact cttactcctt ttacatagtg taacagaaaa gctaaattct
     2401 aggtgtatgg tactagatat ctttgaaaca aaagaaggta aattttttat caacgaaatg
     2461 caaacaatta ttggtgccta tagaccgtat caaatgaaaa taaacaataa accgggaagg
     2521 tttattttaa aagaaaataa ctttattttt gaagaaggta tacattttca aaatgcatgt
     2581 tggaatccaa gagttgaaga tttcaaaaaa atattattag aagagaaata ttatgcataa
     2641 tttaatagct atttttggga catctgcaaa aaaaaatgaa aataggattc cattgcatcc
     2701 tagtttattt catacaatac cacaggaagt aaagaaacat cttatcttcg aaaaaggata
     2761 tggacgaaga ttcaatatta acgatgatta ttttattaaa aactttgctg gggtcgcaga
     2821 aagaaaagat ttatttttaa cttccaacgt ttgggtttta ccaaaaccag agcaagaaga
     2881 ttataaatat ttttctagtg gtaaaatttt atttggttgg ttacactgcg ttcaaggata
     2941 tgaaataacc caagcagcaa ttgaaaataa tatgacctta atttcttggg aatctatgta
     3001 tggcggtaat gacaatacac atatttttaa tcgaaataat gaattagcag gatatgcagc
     3061 tgtacagcat atgttaatgc tacaaggaat aaatggttat tttgggaaaa agctaaaagc
     3121 tgcagtatta ggatttggtg ctactggtcg aggttcaata aatagtctaa aatctctagg
     3181 gattaatgat attactgtat ttagtaatag gcctactttt ctaataaaat cacctattga
     3241 gagtatagat tacaggaaaa taactcataa taataatgat gtttatctag aagatgataa
     3301 aaaaaacata gtagaaccta ttgaagtact taataagttt gatattatca ttaattgtat
     3361 tttacaaaat cctgtatctc caattacatt tattcataat aaagaaataa ataaaattga
     3421 taaaagaaca attataatag atataagctg tgataaaggt atgggatttg agtttgctag
     3481 acccaccaat ttctcaaaac catttataag tataaatgaa aatataaatt attactgtat
     3541 tgataatact ccatctattt attgggaatc cgcatcttat gaaataacaa aatcattaac
     3601 tcctttttta gaattattca taaacaattc ttggaaagat gatatcataa taaaaaatgc
     3661 aatagaaata gaaaatggaa ttataaaaaa taaaaaaata atcgattttc aaaaaagaaa
     3721 taaagattat ccatataaaa tataaaaatg aataataaaa aagaaattat aaataattta
     3781 ttaagttttg ctattataga tgctttaaat ttattaatac caataataac catgcccatt
     3841 ctaactagag cattaggctc tagttcatat ggggattata tgttattaat tactatattg
     3901 acatttggtc atacaatcat tgattatgga gtccaattta ctggtgtcag agatctttcc
     3961 agaaatagaa ataatcaatc tatttattca tctatatatt tagaaaatca agttataaga
     4021 tttatatttt tatttttata tataataatt acaatttttt tcttgataat atcaaaaaaa
     4081 attgagcttt tatattattt tctttatgga ggcgtttttt atttagtagg atatttttta
     4141 gcaagccctt ggttttttca aggaatggga aaaactaaaa tattaatgat tgtgactttt
     4201 atatttaaat tatttaattt agtcggtatt atatttttag ttaaaaacaa agaagacatt
     4261 tatattgctg tttttttatc tacatggact atgtttatca gtggttctat tttatttttt
     4321 atttcaaaaa ataaaattat atttaagaaa tttagctttg aagatttaat cttaagatta
     4381 aagaatagct tcaatgtttt tattggtata ttagctccta atttatacaa ctcagttcca
     4441 actatatatt taggtactat agcaaaacca agttatttcg ctttttatat tgttgcagca
     4501 aggttatgtg atattattgt cgttatacaa aatataatat ctaaatctat ttaccctgtt
     4561 atttcacgta gtaaaaagaa ccacataaaa gttattttat taacaaatat atcactagct
     4621 acacctataa taatatttat atcactgttt ggaagtgatt tattatctct attcttaggt
     4681 aatgaatatt ccaatagtca atcatattta ttgatatttt cttttgggat attattcata
     4741 ggaatagcta attcatttag tcaaggcttt tttctaccta aaaactatga cactatatat
     4801 cgtaaagtat cattaagagt atctgtatta tcagctgtaa tatgctatat attgatcgta
     4861 aaatataatt tgataggtgg agctattgga ataaccatgg caagaatttt attctcatta
     4921 gactacgcag tcacttactt tttaatgaaa aaaaattagt atgaatatat atatttatat
     4981 atgggtattt attgttatta atattttatc acctacgaaa ataaaaaata ataactttgt
     5041 tttcttttca ttttttattt ttttattttt atttattggc ctcagatatg aaataggagg
     5101 ggactggaat aactattcca gaatgtatag taccataagt cagttatcac ttactgaaag
     5161 cttgaaattt acagatcctg catatggatt attaaattgg attgcattca aattttcact
     5221 agggttggta ttcataaata taatatgttc atttatattt ataagtggat tatatttata
     5281 ttctaaagat aaatatgggc gttggctaat tgtattaatt gcatatcctt atttaatatt
     5341 agctgtatcg atggggtata caaggcaagc tgctgcattt ggtttttttc ttctatcaat
     5401 aatagcatta agtcagaata aaccaataaa atttatattt cttatttttc tcggcacctt
     5461 atttcataaa acattaataa tatttatttt tttacttcca tttttcatta aagataaaaa
     5521 gaaaaaaaga aatgtatttc tttctatctt atttatatct ccttttttat ttttagcttt
     5581 ctatgaaaaa atagtaaatc agttgtactt ctattttgaa aatgaattac attcagatgg
     5641 tggactatta agattactta taaatataat cccatcaata ctgttcttta ttttctataa
     5701 tagatggaaa aaatcataca gtgaagatac tcagctcatt ttaattttat ctttactatc
     5761 atttatactt ttagcatttc aatttttcgc gtctacaata agtgatagaa ttggacttta
     5821 tttaattata ttacagttga ttatttatcc taagattctt tatctaataa aagataaagt
     5881 tatacatagt gtgttttctc ttagcattat tgcaatatat agcggcatgt tgtttgtctg
     5941 gttaaattat tctccatggg cacaatgttg ctggattccc tataaaaatt atctatttaa
     6001 ttactaataa attatgataa aaaaacctaa aattattcat attataacag gattaaacaa
     6061 tggtggtgct gaaggtgttt tatatagact gtgcaaatat gatctagcaa atgaccatgt
     6121 agtgatatca ttaaccactg atggaaaata tggtagcatg ttaagagata ttaatgtaaa
     6181 tgttatttgt ttacacttaa aatcgttttc atctacatta ccatctctaa taaaactata
     6241 taaaataata aaaaaagaaa aaccatatat tgtccaaaca tggatgtatc atgcagatct
     6301 tattggagga ataatagcaa aacttgctgg tgtaaaaaaa atatattgga atataagaca
     6361 aacgaacttg ataaaaggga aatcaaaaaa aacaacaatt ttaattgcta agatttgctc
     6421 taaatttagc ttttttatcc cttgtaaaat aatttgttgt gctagtgaag ctaaaaaaac
     6481 acatatagat ttgggatata aaggaaaaga catggttatt atccctaatg gatttgattt
     6541 ttcacaatat aaaaaatcac ataatgatga aataaaaaaa cagttaggaa tacctcagaa
     6601 tagtatatgt attggaatgg ttgctagatt tgatccacaa aaaaatcatc tcttactatt
     6661 agatgcattt acaagaatat ataaaaataa aaatataaaa ctaattttaa ttggtagtaa
     6721 tataacaaat aataataaaa tattagttga atatataaaa aataaaaata ttgaaaaaga
     6781 aactattttg ttgggagaaa gaaaagatat tcctgacatg atgtctttac tagatataca
     6841 tattctaagt agctcttttg gggaaggttt tcctaatgta ttagtagaag caatggctag
     6901 tggaacacct tgtatcgcaa cagatgtagg tgactctaga ttaattattt ctgagtacgg
     6961 atggataatt ccaccaaata atttaaataa attaatatca gcattaaact ctgctttatc
     7021 aattaaagaa ttagataata aaaaatggga agatatatgc gtagcttgta aaaaacatgt
     7081 aatatctaat tatgatatag agaaaatggt tgatgcatac aattctactt ggtttgaaaa
     7141 ataaattatt atgataaaaa tagcccacat acaactatta ccacttctta gtggagctca
     7201 aaaagtttct cttgaagaac ttcaacggtt agatcctaag atatatgatc gttacttaat
     7261 ttgcaaagaa ccaggtcctt tatcttttga agctgaaaaa aataacatta agtgtatcta
     7321 cataaaaaat ttggtaagag aaatatcttt aaaaaatgat atattagcac ttataaaatt
     7381 aataagaata attaaaaaat taaaattcga tattattcat actcactctt caaaacctgg
     7441 agtattaggt agaattgcag catttatatc tagggtacct ttaattattc atactgtaca
     7501 tggttattca tttcctgccg caaaaaataa attacaatat tatatttact taattatgga
     7561 gtatatagga gccaaaagtg gtaacttact aatttgttta catgaaaaag ataaaaatat
     7621 agcaataaaa aaattaagaa taaaagataa aaatattttt gttttagcta atggtgttaa
     7681 tgtagaaaaa tataaaccta atttaaatag gtcattaatt agaaaacgtc tcaacttcaa
     7741 taatgatgac attattattg gtatgacagg tagactatgg gaacagaaaa accctatgct
     7801 cctactatat gcttttgaca aaatcatcaa taaagataat ttaaagttat tatttatcgg
     7861 tgatggtgaa cttaatgaaa aaatgattga ttttattaat gaaaaaaaaa tatcaaataa
     7921 tgttattatg ttaggatggc gagaagacac agatcagata cttaattctt tagatatttt
     7981 tgtgctacct tctaattggg agggcatgcc attagctatt ttagaagcta aaagttgtgg
     8041 tttaccttgt atcgtatcta atatttctgg aaataataat ttaattgata atgagaaaac
     8101 aggtttatta tttgagccaa ataactcaga tgatcttaag ataaaaatag aattattaat
     8161 taataataaa gaaactagag aattatttgg aaatgccaat agagtagata tactaactaa
     8221 ttacaacatt gatgatagaa ttgaaaaaat agacaaactt tatcaatctt ttcttaataa
     8281 gcattagaga ataatataat gaaaatcacc atctcaggaa ccggctatgt cggtctatct
     8341 aatgggatac tcctttccca aaacaacaaa gttgttgcat tagatattaa taaagaaaaa
     8401 gtcgaactat taaacaaaaa aatctctcct atctctgata aagaaattga agagttttta
     8461 gctactaaag agctcgactt tacagcaact ttagataaag aattcgctta taaagatgct
     8521 gaattcgtta tcattgccac tcctaccgat tatgatccta aaactaacta ctttaacacc
     8581 tcttctgtag agtcggttat tcgtgatgtt ttagaatata atccaaacac tactatgatt
     8641 ataaaatcaa ccattccagt tggttttact aaacagatga gagaaaaata tcataccgat
     8701 aatattatct tctctcctga gttcttacgt gaaggccgcg ccctttacga taacctctac
     8761 ccatctcgta ttgtgattgg tgagcaatct gcaagagcag agaaatttgc tgagctttta
     8821 caacaagggg cattaaaaaa agatattgat gttcttttta ctgattctac agaagcagaa
     8881 gccattaagt tatttgctaa tacttatctc gcactgcgtg ttgcttattt taatgaactc
     8941 gatagctacg cccaagctta caatttaaat gcaagacaaa ttattgaagg tgtctgttta
     9001 gatccgcgta ttggtaatca ctataacaat ccatcatttg gttatggtgg ctactgctta
     9061 ccaaaagata cgaaacaact gttagcaaat tacgaatccg tgcctaataa tattattggc
     9121 gccattgtgg aagctaaccg cactcgtaaa gactttattg ctgattctat tatcgctaaa
     9181 tcaccgaaga ttgttggtgt ttatcgttta attatgaaat caggctctga taatttccgc
     9241 tcctcttcaa ttcaaggcat tatgaagcgt attaaagcaa aaggtattga agtggtaatt
     9301 tatgagccag aaatgaaaga agagactttc tttaactcaa aattaattca taatttagat
     9361 gagtttaaag ccatgtctga tgtcattatt accaatagaa tggcttcagc actaaacgat
     9421 gttgaagaaa aagtttatac tcgagacctc tttggtaacg attaattaat agaaacggat
     9481 taattacgta atgaaatatt tagtcacagg tgctgctgga tttattggtt ttcatctaat
     9541 aaaaaaactg atccagcagg gtgaaaccgt tgttggtatt gataacctta atgattatta
     9601 tgatgttgct ttaaaagaag ctagacttaa tcttttaaat caactagata atttctcttt
     9661 ttcttttatt gatttagctg atagagaaaa aattgctcaa ttatttgagg ctgagaaatt
     9721 tgatagggtt attcatttag ctgcacaagc tggtgttaga tattcactaa ttaatccgtt
     9781 ttcttatgct gatagtaatt taactggttt tctaactatt ttagaaggtt gccgacacaa
     9841 taacgttaag catcttgttt atgcatcatc aagttcagtc tatggcttaa atgatgaatt
     9901 gccattttct cctcatgatc aggctaatca tcctgtttct ttatatgcag ccactaaaaa
     9961 agctaatgaa ttaatggcgc atagctattc tcatttatat ggtattccga ctactggatt
    10021 acgttttttc acagtctatg gcccttgggg acgccccgat atggcattat ttaaatttac
    10081 taaagccatt attaacaatc agcctataga tatttataac catggtgaaa tgaaacgtga
    10141 tttcacttat gttgaagata ttgtagaagg tgttacacgt attgctgatg tgatccctac
    10201 agcccagcaa gattggaaag taagtacagg tactccagca gatagttctg caccctataa
    10261 ggtttataat attggtaatg gatctccggt aaatttaatg gattacatta gtgcattaga
    10321 aatacattta ggtaaaaaag cagataaaaa tatgttacca atgcaacccg gtgatgtgta
    10381 caccacatgg gcagatactg aggatttatt taaagcaact ggctataaac ctcaaacaag
    10441 cgttgatgaa ggtgtaaaac agttcgttga ttggtataaa aactattatc aagtaaaata
    10501 aataatgctc aatatcgtct tatttgaacc cgaaattcca ccgaatacgg gtaatattat
    10561 ccgcctgtgt gccaacacag gctttcatct tcatttgatc caaccattgg gttttacttg
    10621 ggatgataaa cggctacgcc gtgcagggct tgattatcgt gagtttgctg atatcaaaca
    10681 gcatcacgat tattatgctt ttttagaaag tgaaggatta agccctgata atgcacaatc
    10741 cccgacaggt gcacggttgt ttgcattaac cactaaaggt actcccgccc atagtaatgt
    10801 aagttatcaa gatggggatt atctgctatt tggtcctgaa acacgaggat taccacctta
    10861 tgtgttggat aatatgccaa cacaacaaaa aatccgtgta ccaatgttag cagatagccg
    10921 cagtatgaat ctttctaata cggttgccgt ggttgttttt gaggcatggc gtcaattagg
    10981 ctatcctggt gcattattaa gagattaatt tatcttttta atttataacc ataaaaaatg
    11041 acctctagac tttattgtct cgaggtcatt tttatgtata tgctttgtgc tagttttagc
    11101 ttttcttatt ttatatcttc tttttatctt ttttatcttt cggttttatt tcttagcttt
    11161 tttagttatt tttcttatca agaaatacca tcttgcactt atttttatta aaaatgatga
    11221 gaaatcagtt actaatacct ttctttctaa taattttttc ttaaattcat ttttttgtat
    11281 taactctttt cgatactctt tagcattaaa ttgttgatta atcttttcaa gtaacctttt
    11341 acagcgtact acttgctcta ctgttttttt atcattatcc aataataatg aataatcctc
    11401 tttattcacg ttaatgccaa aatacattaa taattttcgt cctactcttt ccgcggtatc
    11461 tttttgtatc gctttatatt gcgaacgttc gacatttgga tgagtacgat aataaagaag
    11521 tggtacagga agattatgga gcttaaattt tccagcaata attaaattca cccataaacc
    11581 ataatcttct gcatagaggt ctgctttatc atatccacca acagataata tatcttgctt
    11641 acgatagata atggttggat gcgctaagga gcaatcataa agactagaaa tcacaatatc
    11701 actatgtgtt tctggataag tattaattct atttggctct tcttggtaga taaaagcacc
    11761 accacaaaca ctcacttcag gatgttgtaa cataaattga tattgcgctt ctagtctggt
    11821 tgaaacggca atatcatcag agtccattct agcgactaaa tcacatgaag ctaattgtaa
    11881 tcctttgttt aaagaagcga tcaatccgat attttgttca ttaataatca gttttattct
    11941 gggatctttt ttggcatttt cttttaaaat agataatgag ttatccgttg agccatcatt
    12001 aatgataaga aactcaaatt ctttaaatgt ttgatttaat atagaatcaa tagactcctg
    12061 aaggtatttc tcatcattgt ataccgacat taatacggta attttgggca tattgttttt
    12121 cattatctat tttcagcaat atttattaat ttcaaaattc atcttattga tataccgata
    12181 cccttccccc acatccttta atactaatgc atgggcgcca ataatcgcat tatcaccaat
    12241 attaatctca ccaccaataa tggatgaatt acagccaata taaacatgat ctcctacttg
    12301 gatattggct tttccccctt tctcttttac gccaatagtc accccttgca taatagtgac
    12361 atattccccc agttgacagt aattagtcag tacaatgcca atcgggtgtg gtaaagaaag
    12421 gcctttacca atacgagttt ttaacgagat atcattacag tatttgcggt tattgctagc
    12481 accgatccga atacctaatt tatgtaaaaa gcgatgggga gagaaataaa aatactgtgc
    12541 tacccgccac caaaataggt aacgggaacg ctgacagaaa aatactcgat gaaatagtct
    12601 taaccaacta aagggttttt tatcaccaat atattcagcc tgtaagcact cttttagttc
    12661 agcaagggta accttagctt gagcatgcat agttaaatac catcaccaca ctcaaagccg
    12721 ggtacaacac cattaaagtg ttgatccata tcgaatgacg gtttttcact atcaggacga
    12781 ccgacaatgc gggcaggcac ccccgccacc gtagtatgag ccggtacttc atgtaatact
    12841 accgagccag caccaatttt ggcaccacga ccaatctcga tattaccaag gattttagct
    12901 cctgcgccaa tcattacccc ttcacgcact ttcgggtgac gatcgccccc tgttttacct
    12961 gttcccccta aggtcacaga ttgtaaaatg gagacatcat tttcaaccac cgccgtttca
    13021 ccaatcacaa tacctgtcgc atggtcgagc ataataccgt gtccaatacg tgccgctggg
    13081 tggatatcca ccccaaaggt gactgatatt tggttttgta gataaatagc tagcgcttgg
    13141 cgattttgtt gccatagcca atgggcaata cggtaggctt gtagtgcatg aaaccctttt
    13201 aaatagagca gtggggtaga atatttatcc accgcgggat cacgtaggcg taccgcggag
    13261 agatcacaag cagccgactc aatcattgag ctatcgtgac tataagcctc ttcaacgatt
    13321 tcgcgtactg caatggctgg catagtctga gttgccagct tattggccag catatagctt
    13381 aatgcactac ctaaattttc gtgcttgaga agtgtcgcat gaaagaaact ggctaaaatt
    13441 ggctcacaat ctgccaatgc acgcgcttcg cttttaatat gatcccagac tcttgttagt
    13501 tcttctagcg acatactcac tctcattttt tacacgaaag cctaagtaaa tacttaggct
    13561 ttgaattttc aaacaaatgg atagttaaaa cagataccct aagaatatta tttaaaataa
    13621 actgactaca ggctgctgtc tatctcatct ttggttgctc gccctaataa agccaatgca
    13681 gcctctttta catctttatg ttgatataaa atttggtaga tctgttcggt gattggcatt
    13741 tctacaccca cttgttcggc taatgcgcga acttctttgg tgttacgata accttcgact
    13801 acctggccaa ttttctcttg ggctgtatca acatctaaac cttgacctag catcatacca
    13861 aagcgacgat tgcgtgattg gttgtcagta caggttaaaa ctaaatcacc caaaccagcc
    13921 atgcccataa aagttgccgc atctgcacct aaggctttac ctaagcggct catttcggcc
    13981 agaccacggg taatcagtgc ggtacgcgca ttagcgccaa atcccatacc atcagacata
    14041 cccgcaccaa tagcaatcac gtttttaaca gcgcccccca gttgcacacc gataaaatca
    14101 gggtttttat agactcggaa gcttttacca caatgaaata gctgttgtaa ctgttctaaa
    14161 aagagattat ccgtcgaggc cactgcaatc gcggtgggta aacccgctgc aagctcttta
    14221 gcaaaagtag ggccagataa tactgcgagc gggatttcat tacctaatac ttcacgggca
    14281 acatcttgta ataagcgacc agtatgtgct tcaagacctt ttgtcgccca aacaacacgc
    14341 gcatcctgac gtaaaaaggg tttgatttgt tgcagtactt caccaaacac atggcttggg
    14401 atcaccacaa gaatattacg gctcgcttca atcgcttttt gcaaagaagc ttccatatat
    14461 aaactatcag gaaaggaaac atcgggcaga aaggcttgat tacagcgcgc ttgttctaat
    14521 gccgcaacgt gcttgggatc atgcccccac agcacaacat cgtgcccatt acgcgctaag
    14581 gtaatcgcta aagcggtgcc gtatgaaccg gcaccgataa ctgtcataga agcgttcatt
    14641 aagcttcttc aactcctgag tttgctgcat cctgttgctg ttgttgctgt aagtagttca
    14701 taaacaacgc atcaaagtta acaggtgcta agtttaattg tgggaaagta ccacgaccta
    14761 ctaagttggt gatacattca cgtgcataag ggaataatac gttcgggcag tatgcaccta
    14821 agcaatgtgc taattgtgta ccttcgatac cttcaacagt aaagatacct gcttgttgta
    14881 cttcacacag gaaagc
//
