LOCUS       KY710694               15880 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 28/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710694
VERSION     KY710694.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 15880)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15880)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15880
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 28/57"
                     /serotype="O14ab"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..15880
                     /note="O antigen gene cluster"
     gene            22..816
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             1150..2136
                     /note="orf02"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf02"
                     /product="hypothetical protein"
                     /protein_id="AXY99457.1"
                     /translation="MKKYNILVIGGGNIGHALIYDISDKCNITLIVHEKKKWHTYLEN
                     SKGKLSAKFIVKNWDEPLEKHYNYIFITLPMIYRNNALQLIKRNNIEDSNIIGITGFG
                     NFESLCKEQIKNNNFGSFQRVPYIARIIKYGKKIDILSKKDSLNLYIKDNKEKAKLDL
                     ESIFNVPINLLESHYQNTLSNSNPLLHTSRIYTLFYNESKKYNVFNTEILFYEEWNND
                     ASDMLIKMDNELKEIYKCKNITHLHYSICKHYEVNNIEEMTKKIRSIIAFKNILTPMV
                     KKNNLYYPDLKSRYFIEDFGIGLNSIKNIADTCNLNLPNITKVLNWYNNLEE"
     CDS             2140..3933
                     /note="orf03"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf03"
                     /product="aminotransferase, class I/II"
                     /protein_id="AXY99458.1"
                     /translation="MQVVIPAAGMGNRLGKHTIDKTKCMVKVHDITLIERSLSILAKF
                     NISRIILIVGYHKDKLKNLLGNNFKGIEIIYIDNDIYDKTNNIYSIFLAKDKLVEEDT
                     ILLESDLIFEEKIIENLLSNKFPNLAVVDKYQPWMDGTVVEINDHFDITSFITKKDFN
                     FNKTNDYYKTVNIYKFSKEFLKNTYVPFLEAYSKALGQNEYYEQVLRVITLLETQELK
                     ALPLQNEKWYEIDDIQDLDNAEVIFSPYEKKLSLLNKRYGGYWRYNELIDFCYLVNPY
                     FPNKTFMGEMHNSFDTLVREYPSGADIQSLLASKLFKCKKEQIVVGNGAAELIDKLAN
                     HIDLKLGLFIPTFEEYSARFKNIQLERITNQDYCYTKNDIIRLANKVDGVILINPDNP
                     SGNYISYKDVIELITYFKKEKKLLIIDESFIDFAEEGLHASLLSEDILNSYNGLIIIK
                     SISKSYGVPGIRLGVLASSNVDLISKLKKDIPVWNINSLGEFFLQIIGKYEKPYQKSC
                     SYIIKERNFLFEQLKSIKFLRPIKSQANYILCEVINKDSAEVVSALCNRFNIIAKDCS
                     NKKGFENKNYIRLAVRNSSENQYLIDSLKEI"
     CDS             3945..4334
                     /note="orf04"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf04"
                     /product="hypothetical protein"
                     /protein_id="AXY99459.1"
                     /translation="MYKLELINLELIQETEEHIPDRVIWLKEKILEEGTWKVPLLLEE
                     NSLAIMDGHHRFNVAKKLGLKRIPAILLSYNSPYVSITSWREDFPVNKEIILDYIKYK
                     KKFPLKTTRHIISPYPEEISIPINFLY"
     CDS             4360..5592
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99460.1"
                     /translation="MLKKNIVNLFINQIFSYVAPLLLLPFLSRTLDVTHFGLYIFSLS
                     TISIATVITNYGFDISVAKRIAEGENKKENLNEFLYQVNIIKSFICFFTIIIVAYILY
                     ATNYFTDNPTNIYIILLSIVFSSYNLIWLFQGLEKIYIYSRITIVTRLLSMFLIIITI
                     KSSSDFSLLLLLNFLQILVSIIASYYIIFNWNLKFKTCSIKKSIFLFKESTEYFISRV
                     GVTLYSSACSFFLGIFSGSLHQVAIYGTAEQLYRAGVYLMSAISSPLTPYMARTKNYT
                     IFWKIVVFTLIITILGASIGFVFGDDIIRLIYGSKFNDSYSILNVFMLTIIISVMGMF
                     FGYPALIPIGKTKIANYSVLYAGLLQLIILLIMYILHIEFTAIKIAFSYFLCEIFVLI
                     IRLIYFLPNYKIMKNEKH"
     CDS             5579..6412
                     /note="orf06"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf06"
                     /product="LicD-family phosphotransferase"
                     /protein_id="AXY99461.1"
                     /translation="MKNIELSEVQQILVNALKHIHSICEKHNIKYWLDSGTLLGAVRN
                     NKFISWDDDIDICMLREDFNKFLVIALSELDKDKYFLQTCNTDPYYKEYDIPCKVRVN
                     NTYIEESKKIFKNNLNLKAHNGFFIDIFPCDKYPNNKFLRDFFQSISVIYKAKSLFFR
                     KSLTKKAFFSSRIIHYIIPINLLNYLNLKVTNYLNNNSKKHPIISKGIELPFHKGNIP
                     YSEIFPLKKILFEGSFFYVPKNTNYYLISLYGNDYLTPPDINNRLVHSSVFKKKSKNI
                     S"
     CDS             6449..7507
                     /note="glycosyltransferase"
                     /gene="gt1"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AXY99462.1"
                     /translation="MKKIIFFIESLENSGGTERITTLIANSLAEKNFSVSIITIIGEH
                     TPFFPVNKKISIYHLSRNIKKNPFYIFFAVKKLKNLLKELNAEYIITVGSINSIYTIP
                     ATLGTKVKNFCWEHFNYTIGRGVKLLRKISRISCKKIIVLTHKDKILWEKYTYGKAKI
                     TIINNPSTFKKQSTLPLIKNKTVISIGRLEPQKGFDKLINIWEKSELKDYGWKLFIVG
                     NGKEYNNLLSLISEKNLNNNIKILPPTKNINKIYKSASIYCMTSRFEGMPLVLLEALS
                     FNLPLIAYDCNTGPSEIITSDENGLLIENTNTEHYISALSKLALNESYYNQVTSNILI
                     KNYFSIDKIITEWVNNFD"
     CDS             7500..8516
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99463.1"
                     /translation="MIRIKYSTIFIVFFCISYATLYASLDSTFFRDRLSYSGYYATSS
                     FIMLNKAYDISFIKLLTEEPLFLLLNILLTKVFFVSNELLPHVYVFFISFIFSFYIIK
                     KSYNLNFLILSILMLLTVSLSFHAQLVVLRQSLATSILLIFICNKYNLNKLIFISFIC
                     ALIHNSFFILLLYFLMIRYTNNDIKKNKIIFISIFISLIATTSFYVIAHYIQIRQVNY
                     LSSNNGNSGSGLILWGSCLLVILLKNRHIKKISIIDSLSIFGLFFYIINYYFLPIGGR
                     LLITFIPFIFISLTSGFYHKKTLPLILIFNMLFIISNIYKLNDNTKNNSLNGNYQIYK
                     LIGS"
     CDS             8524..9504
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99464.1"
                     /translation="MLFSVIIPCFNSENHISTCLKYLLAQINSDDDIEVLLVDDGSND
                     NTLSIISEYKEKYPYYIKIIKNEKNRGVSYSRNVGIYNSIGKFILFLDSDDQYKDGLF
                     IELRNIIKKSSDIDIVSFSFLREGGKDKSETIYSFKEFNKKIFYNIDFFKLFCIKKIN
                     QHICSAAFRRELIISNNIFFDLGTYIGEDVEFQIKSILYAKKIYYSSTIFFIYKYNSQ
                     SVTNNKLSEKHLSFGKIYPRLKKIISDKNLIPYLSFYYTYSFFYILYSFSEDKNLVNK
                     FSIYDSILKDRMYINISNKKSLITFISKVVYKINKKIFLSLLNFFKRIST"
     CDS             9501..10340
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99465.1"
                     /translation="MSYKMENITPEIAIIMSVYKNDKLLYIQQAVESILNQKNVNYLF
                     FMHLDGKVNSVVMDYLNKLQHSNKIILSQSILNNGLSNSLNYLIDKVLIIPNIKYIAR
                     MDSDDISLPERLSKQVRFLENHPNIDVLGTACKEFGSSFSLEKKFLPSHHDDLVKYSI
                     CRCPFIHPTVMFRRKVFDDGNRYPTHTTFTEDMAFWLELIYKGYKLHNLPDVLLQYRL
                     EDNTMHRRKGLSKAKSEFSLRMGYMFLLNQVSLKNILLISSRYIFHLLPPSIMSKAYK
                     YLR"
     CDS             10402..11568
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99466.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLDAFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     CDS             11580..12083
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemG"
                     /product="wemG"
                     /protein_id="AXY99467.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNVQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(12221..13198)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXY99468.1"
                     /translation="MKNNMPKITVLMSVYNDEKYLQESIDSILNQTFKEFEFLVINDG
                     STDNSLSILKENAKKDPRIKLIINEQNIGLIASLNKGLQLASCDLVARMDSDDIAVST
                     RLEAQYQFMLQHPEVSVCGGAFIYQEEPNRINTYPETHSDIVISSLYDCSLAHPTIIY
                     RKQDILSVGGYDKADLYAEDYGLWVNLIIAGKFKLHNLPVPLLYYRTHPNIDRSQYKA
                     IQKDTAERVGRKLLMYFGINVNKEDYSLLLDNDKKTVEQVVRCKRLLEKINQQFNAKE
                     YRKELIQKNEFKKRLLERKILVTDFSSFLIKVSARWYFLIRKITKKAKK"
     CDS             complement(13208..13765)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemH"
                     /product="wemH"
                     /protein_id="AXY99469.1"
                     /translation="MHAQAKVTLAELKECLQAEYIGDKKTFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGLRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGDHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVEEGCRYINKMNFEINKYC"
     CDS             complement(13768..14601)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99470.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPMLASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(14739..15713)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99471.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVESYRNTKEVRALAEQVGVEMPNHRTDYQILYQHKDVKEAAWLY"
     gene            complement(15713..15880)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5667 a   2433 c   2393 g   5387 t
ORIGIN
        1 ctcaaaaatg cggcggatga tgtggcaaaa ggtaacctgc gtcctcaccc tgagctggaa
       61 accggcccac aagaattttt agcggcaggc accagtttta atcagatgat cagcgcatta
      121 gagcgtatgg ttgaagcgca acagcgattg atttctgata tctcccatga gttgcgcacc
      181 ccattaactc gtttacagct tgccagtgca ttactacgcc gtcgtagcgg tgaaagtaaa
      241 gagctagagc gtattgaaac agaaacacag cggctagatg gcatgatcaa tgacttatta
      301 gtgctttctc gcaaccagta taaaaatgag ttattacgtg aaaccgtaaa agcgaatgag
      361 ctttggaacg atattttaga taatgcaaaa tttgaggctg aacagagcaa taaaacctta
      421 acagtaaccg ccccgccagg cccatggacg atttattgca atccttactc attagcgagt
      481 gcctttgaaa atatcgttcg caatgccttg cgttattcac attctcgtat tgaagtggcc
      541 tttaccgaac aaaatcaggg tatcactatt attgttgatg atgatggccc aggggttagc
      601 ccagaagatc gcgagcatat tttccgacct ttttatcgta cggacgaagc acgtgataga
      661 gaatccggcg gtacagggct gggactggca attgttgaaa ccgcgattag tcaacatcga
      721 ggccatgtaa aagccgatga tagcccatta gggggattaa gagtggagat ttggttgccg
      781 agagggagtg tggtaaataa atcacaacac gcttaattca gtaataaaat tactatatta
      841 aattattatg ccatctattg aagaataggt ggcatattct tttcattccc atctttatag
      901 atatttcaga atgatcttgc taattcagtc gatattccga tatttatata tacctaatca
      961 atgttagaat acccatcaat tacttgaccc gcgggtttga agagtctaat ctcagagcgg
     1021 ttttacgtac tgttaaagta tacttatata ataaaactct tatattacag atagataagg
     1081 gttttaaaag agaaattttg gcgctctggg ggccgtaaac ccagtgcggt agctatgtaa
     1141 gagcgaaaaa tgaaaaaata taatatatta gttattggtg gtggtaatat cggtcatgca
     1201 ttaatatatg atatttctga taaatgtaat ataaccctta ttgtacatga gaaaaaaaaa
     1261 tggcatacat atctagaaaa ctctaagggg aaattaagtg ccaagtttat tgtaaaaaat
     1321 tgggatgaac ctctagaaaa acattataat tatatattta ttacattacc tatgatatat
     1381 agaaataatg cacttcaatt aattaagaga aataatattg aagatagcaa tataattgga
     1441 attacaggat tcggaaattt tgaatcatta tgcaaagaac aaataaaaaa taataacttt
     1501 ggttcattcc aacgagttcc atatatagca agaattataa aatatggaaa aaaaatagat
     1561 attttaagta aaaaagactc gttgaactta tatataaaag ataataaaga aaaggcaaaa
     1621 ctagatttag aatctatttt taatgttcca atcaatttac ttgaaagcca ctatcaaaat
     1681 acattatcca attcaaatcc attattacac acatctagaa tctatacatt attttataat
     1741 gaaagtaaaa aatataatgt ttttaataca gagatattat tttatgaaga gtggaataat
     1801 gatgcttcag acatgctaat aaaaatggat aatgaattaa aagaaatata taaatgtaaa
     1861 aatataaccc atttacacta cagcatatgc aagcattatg aggttaataa tattgaagaa
     1921 atgactaaaa aaataagatc tattatagca tttaaaaata tattaactcc aatggtgaaa
     1981 aaaaataatc tttattatcc ggatttaaaa tcaaggtatt ttatagagga ttttggtatt
     2041 gggttaaata gcataaaaaa tatcgcagat acatgtaatt taaacttacc taatattaca
     2101 aaagttctta attggtataa caacttagag gaataacaaa tgcaagtagt aattccagct
     2161 gcgggtatgg gcaatagatt aggaaaacat acaatagata aaacaaaatg tatggtcaaa
     2221 gtccatgata tcacacttat tgaacgtagt ttatcaatat tagctaaatt taatatttca
     2281 agaatcatac tgatagtagg ataccataaa gataaattaa aaaatctcct aggaaataat
     2341 tttaaaggta tagaaataat atatatagat aatgatattt atgataaaac aaataatatt
     2401 tattctattt ttttagctaa agataaatta gtagaggaag atacaatact acttgaaagt
     2461 gatttaattt ttgaagagaa aatcattgaa aatcttctct ccaacaagtt tcctaatctt
     2521 gctgtagttg ataaatatca accatggatg gatggaacag ttgttgaaat aaatgatcat
     2581 ttcgacatca caagttttat aacaaagaaa gattttaatt ttaacaaaac taatgattat
     2641 tataaaacgg ttaatatata taaattttca aaagaatttt taaaaaatac atatgttcct
     2701 tttttagaag cctactcaaa agcacttgga caaaatgaat attatgaaca agttttgaga
     2761 gttataacat tattagagac acaagaatta aaagcattac cattacaaaa cgaaaaatgg
     2821 tatgaaattg atgatattca agatctagat aatgcagaag taattttttc tccctatgaa
     2881 aaaaaattat cattattaaa caaacgttat ggtggatatt ggcgttataa tgaactaata
     2941 gatttttgct atctagttaa tccgtatttc cctaataaaa cttttatggg agaaatgcat
     3001 aattcctttg atactctagt tcgagaatac ccttcaggag ctgatattca atctttatta
     3061 gcatcaaagc tatttaaatg caaaaaagaa caaatagttg taggtaatgg tgcggcagaa
     3121 ttaatagata aactagcaaa tcatatagac ctaaaattag gcttatttat tccaacattc
     3181 gaagagtatt cagctagatt caaaaatata caattagaaa gaatcactaa tcaggattat
     3241 tgttacacaa aaaatgatat tattcgatta gctaataaag tcgatggtgt aatattaata
     3301 aaccctgata atccgtcagg aaattatatt tcttataaag atgtaataga acttatcacc
     3361 tattttaaaa aagaaaaaaa attattaata atagatgaat catttattga ttttgcagaa
     3421 gaagggttgc atgctagctt attatcagaa gatatattaa atagttataa tggcttaatc
     3481 ataattaaaa gtataagcaa aagctatgga gttcctggta ttagattagg tgttttagca
     3541 tcatctaatg ttgacctaat atcaaaatta aaaaaagata tacctgtctg gaatataaat
     3601 tcattaggag aattttttct acaaataata ggaaaatatg aaaaacctta tcaaaaatct
     3661 tgtagttata ttataaaaga acgaaatttt ctttttgaac aactaaaatc gataaaattt
     3721 ttaaggccta taaaatcaca agctaattat attttatgtg aagttataaa taaagattca
     3781 gccgaagttg tatcagcact atgcaataga tttaatataa ttgcaaaaga ctgttctaat
     3841 aaaaaaggat ttgaaaataa aaattatata agactagctg tcagaaactc ttcagaaaac
     3901 caatacttaa ttgatagttt aaaagaaata taaaggaata tttcatgtat aaattagagc
     3961 ttattaatct tgaattaata caagaaactg aggagcatat tcctgatcgt gtaatatggt
     4021 taaaagaaaa aatcttggag gaaggaacct ggaaggttcc tcttttatta gaagaaaatt
     4081 cattagcaat aatggatgga catcatcgtt ttaatgtagc taaaaaactg ggattaaaaa
     4141 gaattcctgc tattttatta tcttataact cgccatatgt tagtattact tcatggagag
     4201 aagactttcc tgtaaataaa gaaattattc ttgattatat aaaatataaa aaaaagtttc
     4261 cattaaaaac aacaagacat attatcagtc cataccctga agaaatatca atcccaataa
     4321 attttcttta ttaaaaatat aatatagctt aatttttata tgctaaaaaa aaacatagta
     4381 aatctattta taaaccaaat atttagttat gttgcacctt tgcttctttt accattttta
     4441 agtagaacat tagatgtgac tcattttggt ctatatattt tttcattatc tacaatatca
     4501 attgcaacag ttataaccaa ttatggtttt gatatatcag tagctaaaag aatcgcagag
     4561 ggagaaaata aaaaagaaaa tttaaatgaa tttttatatc aagttaatat tataaaatca
     4621 tttatttgtt tttttacaat aataattgtt gcatatattc tttatgctac taattacttt
     4681 acagataatc caaccaatat atacattatc ctattgtcaa tagtattttc ttcttataat
     4741 cttatttggt tatttcaagg attagaaaag atatatatat atagtagaat aacaattgtt
     4801 acacgattgt tatctatgtt tttaatcata ataactataa aatcctcttc tgactttagt
     4861 ttattattgc ttttaaattt tcttcagatc ttggttagta ttattgccag ctactacata
     4921 atatttaatt ggaatttaaa atttaaaaca tgctctataa aaaaatctat ttttttattt
     4981 aaagagtcaa ccgaatactt tatttcaaga gttggagtaa ccttatattc atcggcctgt
     5041 agtttcttct tgggaatatt ttctggttca ttacatcagg tagcaatata tgggacagca
     5101 gaacaattat atagagcggg agtatatcta atgagcgcca tttcctctcc attaacacca
     5161 tatatggcaa gaacaaaaaa ttatactatt ttttggaaaa tagtagtatt tacattaata
     5221 ataacaatat taggggcaag tattggtttc gtatttggtg atgatattat taggttaatt
     5281 tatggtagca aatttaatga ttcatattct attttaaatg tttttatgct aactattatc
     5341 atcagtgtta tgggaatgtt tttcggttat ccagcattaa ttccaatagg aaaaactaaa
     5401 attgctaatt atagtgtatt atacgcaggt cttttacaat taataatact attaattatg
     5461 tatatactgc atattgaatt tacagcaata aagatcgctt ttagttattt cttgtgtgaa
     5521 atttttgtgt tgataattcg attaatatat tttttaccaa attacaaaat aatgaaaaat
     5581 gaaaaacatt gaactctctg aagtacaaca aatattagtt aatgctttaa agcatataca
     5641 cagtatctgt gaaaaacaca atataaaata ttggctagat tcaggaacat tattaggtgc
     5701 agttagaaat aataagttta tatcttggga tgacgatatt gatatctgca tgcttcgtga
     5761 ggattttaat aaatttttag ttattgcatt aagcgaacta gataaggata agtatttctt
     5821 gcaaacatgt aatacagatc cttattataa ggaatatgat ataccatgca aagtgagggt
     5881 aaataataca tatatagaag aaagtaaaaa aatatttaaa aacaatttaa acttgaaggc
     5941 acacaatggt ttttttattg acatatttcc atgtgataaa tatccaaata ataaattttt
     6001 aagggatttt tttcagtcaa tatctgttat ttataaggct aaaagtttat tttttagaaa
     6061 atcactaact aaaaaagctt ttttttctag tcgtattatt cactatataa ttcctataaa
     6121 tttattaaat tatttaaatt taaaagtaac taactattta aataataatt caaaaaaaca
     6181 tcctattatc tctaaaggta ttgagctccc ttttcataaa ggaaatattc cttatagtga
     6241 aattttccct ctaaaaaaaa tattatttga aggttctttt ttttatgttc ccaaaaatac
     6301 aaattactat ttaatttcat tatacggtaa tgattattta actcctcctg atataaataa
     6361 tagattagta cattcatcag tatttaaaaa aaagtctaaa aatatttctt agtttataga
     6421 attaacttga cctatataat tatatattat gaaaaaaatt atttttttta ttgaaagtct
     6481 agaaaatagt ggtggtacag aaagaattac cactttaatt gcgaattctc ttgcagaaaa
     6541 aaattttagt gtttctataa taacaataat aggagagcat acaccttttt tccctgtgaa
     6601 taaaaaaatt agtatttatc atttaagtag aaatataaaa aagaaccctt tttatatttt
     6661 ttttgctgta aaaaagctaa aaaatttatt aaaagaactt aatgctgaat atattattac
     6721 tgttggtagt attaatagta tatatacaat ccctgctaca ttgggaacta aagttaagaa
     6781 tttttgttgg gaacatttta attatactat tgggagggga gtaaaattat taagaaaaat
     6841 atcaaggatt tcttgtaaaa aaatcatagt attaacacat aaagataaaa tattatggga
     6901 aaaatataca tatggtaaag caaaaattac cattataaat aacccttcta cctttaaaaa
     6961 acaatcaaca ctccctctca ttaaaaataa aacagtaata tctattggaa gattagaacc
     7021 tcagaaagga tttgataaac taataaacat atgggaaaaa tctgaactta aagactatgg
     7081 atggaaactt ttcattgtag gaaatggaaa ggaatataat aatttattat ctttgatatc
     7141 agaaaaaaat ttaaataaca atataaaaat attacctcca acaaaaaata ttaacaaaat
     7201 atataaatct gcatctatat attgtatgac atcaagattt gaaggtatgc ctttagtttt
     7261 actagaggca ttatctttca atttgccgct aatagcttat gattgtaata ctggacctag
     7321 cgagataatt acaagtgatg agaacggact actaatagaa aatactaata cagaacacta
     7381 tattagtgct ctatcaaaat tagctttaaa tgaaagttac tataaccaag taacatctaa
     7441 tatcttaata aaaaactatt tttctataga taaaataatt actgagtggg taaataattt
     7501 tgattagaat aaaatactca acaatattta ttgttttttt ttgtatcagt tatgctacgc
     7561 tttatgctag tttagattct acttttttca gagatagatt atcttatagc ggttattatg
     7621 caacaagcag ttttattatg ctaaataagg catacgatat ttcttttata aaattactta
     7681 cggaagaacc tttatttctt cttttaaaca ttttgctgac aaaagttttc ttcgttagta
     7741 atgagttatt accccacgtt tatgtttttt ttatatcatt catattttct ttctatataa
     7801 taaaaaaaag ctataatctt aattttctaa tattaagtat acttatgtta cttactgttt
     7861 ctttatcgtt tcatgctcaa ttggttgtat taagacaatc cctagcgact agtattctat
     7921 taatattcat atgtaataaa tataatttaa ataaattaat ttttatttct tttatttgtg
     7981 cattaattca taattctttt tttatcttgt tactatattt cttaatgata agatatacta
     8041 ataatgatat aaaaaaaaat aaaataatat ttatatcaat ttttattagc ttaatagcaa
     8101 caacatcttt ctatgtcata gctcattata ttcaaataag gcaagtaaat tatttatcct
     8161 ctaataatgg aaatagtgga agtggtctaa ttctttgggg gagttgttta ttagttattt
     8221 tattaaaaaa taggcatatt aagaaaataa gcataataga ttcattatct atttttggat
     8281 tattttttta tattataaac tattattttt tgcctattgg cggaagactt cttattacat
     8341 ttattccatt tatttttatt tcattaacat ctggatttta tcataaaaaa actctacctt
     8401 taattcttat ttttaatatg ttatttataa tatctaacat atataagcta aatgataata
     8461 caaaaaataa tagtcttaat ggtaattacc agatttataa attaatagga tcatgattta
     8521 aaaatgcttt tctcagttat tataccatgt tttaattccg aaaatcatat atcaacttgc
     8581 ctcaagtatc ttttagcaca aataaattct gatgatgata tagaagtgct ccttgttgat
     8641 gatggttcta atgataatac tctatctatt atatctgaat ataaagaaaa atatccttat
     8701 tatataaaaa taattaaaaa cgaaaaaaat cgaggagtat cttatagcag aaatgtcggc
     8761 atatataatt caattggcaa atttatttta tttttagatt ctgacgatca atataaagat
     8821 ggtctattta tagaattaag aaatataata aaaaaatcct cagatattga tattgtgagt
     8881 ttttcttttc taagagaagg agggaaagat aagtctgaaa caatatattc ttttaaagaa
     8941 tttaataaaa aaatatttta taatatagat tttttcaaac ttttttgtat aaaaaaaata
     9001 aatcaacata tttgctctgc tgcattcaga agagaactta tcatatcaaa taatattttt
     9061 tttgatctag gtacatatat aggagaagac gttgagtttc aaattaaatc aattttatat
     9121 gcaaaaaaaa tatattattc gtctactatt ttctttatat ataaatataa tagccaatca
     9181 gtgactaata acaaattatc agaaaaacat ttatcttttg ggaaaatata ccctagatta
     9241 aaaaaaataa tctctgataa aaatttaatt ccttatttat ctttttatta tacatattca
     9301 tttttctata tcttatattc tttttctgaa gataaaaatt tagttaataa gtttagcata
     9361 tatgatagta tattaaagga tagaatgtat ataaatataa gcaataaaaa atctcttatt
     9421 acttttattt ctaaagttgt atataagata aacaaaaaaa tatttttatc attactgaat
     9481 ttttttaaaa gaatatctac atgagttata aaatggaaaa tatcactcct gaaatagcaa
     9541 ttattatgag tgtttacaaa aatgataaac tcttatatat acaacaagct gttgagtcta
     9601 ttctaaacca gaaaaatgtc aattacttat tttttatgca cttagatgga aaagtaaata
     9661 gtgtcgtaat ggattattta aataaattgc aacatagtaa taaaattatt ttatctcaaa
     9721 gtatactaaa taatggatta tctaattctc ttaattattt aatagataaa gtattaatta
     9781 ttccaaatat aaaatatatt gctagaatgg atagtgatga tatatctctt cctgaaaggc
     9841 tttcaaaaca agtaagattt ttagaaaatc accctaatat agatgtactt ggtactgcat
     9901 gtaaggaatt tggctcttca ttctcattag aaaaaaaatt cttaccttca catcatgatg
     9961 atttggtcaa atacagtatt tgtcgttgcc cttttattca tccaactgta atgtttagac
    10021 gaaaagtctt tgatgatggt aatcgttacc ctacacatac tacatttact gaagatatgg
    10081 ctttttggtt agaattaata tataaaggct ataaactaca taatttacca gatgtgttgt
    10141 tacaatatcg attagaagac aacacaatgc atagaagaaa aggattatca aaggcgaaaa
    10201 gtgaattttc tttgcgtatg ggatatatgt tccttctaaa tcaagtatca ttaaaaaata
    10261 tattactaat tagctctaga tatattttcc atttattacc acctagtata atgagtaaag
    10321 catataaata tttaagatag agatatttcc atcttctatt ctatacaatc ctatttgata
    10381 aattttagat aagaaaaaca catgaaaatc accatctcag gaaccggcta tgtcggtcta
    10441 tctaatggga tactcctttc ccagcacaac aaagttgttg cattagatat taataaagaa
    10501 aaagtcgaac tattaaacaa aaaaatctct cctatctctg ataaagaaat tgaagagttt
    10561 ttagccacta aagagctcga ttttacggca actttagata aagaattcgc ttataaagat
    10621 gctgaatttg ttatcattgc cactcctacc gattatgatc ctaaaactaa ctactttaac
    10681 acctcttctg tagagtcggt tattcgtgat gttttagaat ataatccaaa cactactatg
    10741 attataaaat caaccattcc agttggtttt actaaacaga tgagagaaaa atataatact
    10801 gataatatta tcttctctcc tgagttctta cgtgagggtc gcgcccttta cgataacctt
    10861 tatccatctc gtattgtgat tggtgagcaa tctgcaagag cagagaaatt tgctgagctg
    10921 ttacaacaag gggcattaaa aaaagatatt gatgttcttt ttactgattc tacagaagca
    10981 gaagctatta agttatttgc caatacttat ctcgcactac gtgttgctta ttttaatgaa
    11041 ctcgatagct acgcccaagc ttacggctta aacgcaagac aaattattga aggtgtttgt
    11101 ttagatccgc gtattggcaa tcactataat aacccctcat tcggttatgg cggttactgc
    11161 ttaccaaaag atactaaaca actgttagcc aactacgaat ccgtgcctaa taatattatt
    11221 ggcgccattg tggaagctaa ccgcacccgt aaagacttta ttgctgattc tattattgct
    11281 aaatcaccaa agattgttgg tgtttaccgt ttaattatga aatcaggctc tgataatttc
    11341 cgctcatctt caatccaagg cattatgaag cgtattaaag caaaaggcat tgaagtagtg
    11401 atttatgagc cagaaatgaa agaagagact ttctttaact caaaactcat tcgtgattta
    11461 gatgcgttta aagccatgtc tgatgtcatt attaccaata gaatggcttc tgcactaaac
    11521 gatgttgaag aaaaagttta tactcgtgat ctctttggta acgattaaaa ctaatttata
    11581 tgctcaatat cgtcttattt gaacccgaaa ttccaccgaa tacgggtaat attatccgcc
    11641 tgtgtgccaa cacaggcttt catcttcatt tgatccaacc attgggtttt acttgggatg
    11701 ataaacggct acgccgtgca gggcttgatt atcgtgagtt tgctgatatc aaacagcatc
    11761 acgattatta tgctttttta gaaagtgaag gattaagccc tgataatgta caatccccga
    11821 caggtgcgcg gttgtttgca ttaaccacta aaggtactcc cgcccatagt aatgtaagtt
    11881 atcaagatgg ggattatctg ctatttggcc ctgagacacg aggtttacca ccttatgtgt
    11941 tggataatat gccaacacaa caaaaaatcc gtgtaccaat gttagcggat agtcgcagta
    12001 tgaatctttc taatacggtt gccgtggttg tttttgaggc atggcgtcag ttaggctatc
    12061 cgggggcatt attaagagat taatttatct ttttaattta taaccataaa aaatgacctc
    12121 tagacttcat tgtctcgagg tcatttttat gtatatgctt tgtgctagtt ttagcttttc
    12181 ttattttata tcttcttttt atctttttta tctttcggtt ttatttctta gcttttttag
    12241 ttatttttct tatcaagaaa taccatcttg cacttacttt tattaaaaat gatgaaaaat
    12301 cagttactaa tatctttctt tctaataatc ttttcttaaa ttcatttttt tgtattaact
    12361 cttttcgata ctctttagca ttaaattgtt ggttaatctt ttcaagtaac cttttacagc
    12421 gtactacttg ctctactgtt tttttatcat tatccaataa taatgaataa tcctctttat
    12481 tcacgttaat gccaaaatac attaataatt ttcgtcctac tctttctgcg gtatcttttt
    12541 gtatcgcttt atattgcgag cgatcgatat ttggatgagt acgataataa agaagtggta
    12601 caggaagatt atggagctta aattttccag caataattaa gttcacccat aaaccataat
    12661 cttctgcata gaggtctgct ttatcatatc caccaacaga taatatatct tgcttacgat
    12721 agataatggt tggatgcgcc aaggagcaat cataaagact agaaatcaca atatcactat
    12781 gtgtttctgg ataagtatta attctatttg gctcttcttg gtagataaaa gcaccaccac
    12841 aaacactgac ttcaggatgt tgtaacataa attgatattg cgcttctagt ctggttgaaa
    12901 cggcaatatc atctgagtcc attctagcga ctaaatcaca tgaagctaat tgtaatcctt
    12961 tgtttaaaga agcgatcaat ccgatatttt gttcattaat aatcagtttt attctgggat
    13021 cttttttggc attttctttt aaaatagata atgagttatc cgttgagcca tcattaatga
    13081 caagaaactc aaattcttta aatgtttgat ttaatataga atcaatagac tcctgaaggt
    13141 atttctcatc attgtatacc gacattaata cggtaatttt gggcatattg tttttcatta
    13201 tctattttca gcaatattta ttaatttcaa aattcatctt attgatatac cgacaccctt
    13261 cctccacatc ctttaatact aatgcatggg cgccaataat cgcattatca ccaatattaa
    13321 tctctccacc aataatggat gaattacagc caatataaac atgatctcct acttggatat
    13381 tggcttttcc ccctttctct tttacgccaa tagtcacccc ttgcataata gtgacatatt
    13441 cccccagttg acagtaatta gtcagtacaa tgccaatcgg gtgtggtaaa gaaaggcctt
    13501 taccaatacg agtttttaac gagatatcat tacagtattt acggttattg ctagcaccga
    13561 tccgaagacc taatttatgt aaaaagcggt ggggagagaa ataaaaatac tgtgctaccc
    13621 gccaccaaaa taggtaacgg gaacgctgac agaaaaatac tcgatgaaat agtcttaacc
    13681 aactaaaggt ttttttatca ccaatatatt cagcctgtaa gcactctttt agttcagcaa
    13741 gggtaacctt agcttgggca tgcatagtta aataccatca ccacactcaa agccgggtac
    13801 aacaccatta aagtgttgat ccatatcgaa tgacggtttt tcactatcag gacgaccgac
    13861 aatgcgggca ggcacccccg ccaccgtagt atgagccggt acttcatgta atactaccga
    13921 gccagcacca attttggcac cacgaccaat ctcgatatta ccaagaattt tagctcctgc
    13981 gccaatcatt accccttcac gcactttcgg gtgacgatca cctcctgttt tacctgttcc
    14041 ccctaaggtg acagattgta aaatagagac atcattttca accaccgccg tttcaccaat
    14101 cacaatacct gtcgcatggt cgagcataat accgtgtcca atacgtgccg ctgggtggat
    14161 atccacccca aaggtgactg atatttggtt ttgtagataa atagctagcg cttggcgatt
    14221 ttgttgccat agccaatggg caatacggta ggcttgtagt gcatgaaacc cttttaaata
    14281 gagcagtggg gtagaatatt tatctaccgc gggatcacgt aggcgtaccg cggagagatc
    14341 acaagcagcc gactcaatca ttgaactatc gtgacgataa gcctcttcaa cgatttcgcg
    14401 tactgcaatg gctggcatag tctgagttgc cagcttattg gccagcatat agcttaatgc
    14461 actacctaaa ttttcgtgtt tgagaagtgt cgcatgaaag aaactggcta acattggctc
    14521 acaatctgcc aatgcacgcg cttcgctttt aatatgatcc cagactcttg ttagttcttc
    14581 tagcgacata ctcactctca ttttttacac caaagcctaa gtaaatactt aggctttgaa
    14641 ttttcaaaca aatggatagt taaaacagat accctaagaa tattatttaa aataaactga
    14701 ctacaggctg ctgtctatct catctttggt tgctcgccct aataaagcca tgcagcctct
    14761 tttacatctt tatgttgata taaaatttgg taatctgttc ggtgattggg catttctaca
    14821 cccacttgtt cggctaatgc gcgaacttct ttggtgttac gataactttc gactacctgg
    14881 ccaattttct cttgggctgt atcaacatct aaaccttgac ctagcatcat accaaagcga
    14941 cgattgcgtg attggttgtc agtacaggtt aaaactaaat cacccaaacc agccatgccc
    15001 ataaaagttg ccgcatctgc acctaaggct ttacctaagc ggctcatttc ggccaggcca
    15061 cgggtaatca gtgcggtacg cgcattagcg ccaaatccca taccatcaga catacccgca
    15121 ccaatagcaa tcacgttttt aacagcgccc cccagttgca caccgataaa atcagggttt
    15181 ttatagactc ggaagctttt accacaatga aatagctgtt gtaactgctc taaaaagaga
    15241 ttgtctgtcg aggccactgc aatcgcggtg ggcaaacccg ccgcaagctc tttagcaaaa
    15301 gtagggccag ataatactgc gagcgggatt tcattaccta atacttcacg ggcaacatct
    15361 tgtaataagc gaccagtatg tgcttcaaga ccttttgtcg cccaaacaac acgtgcatcc
    15421 tgacgtaaaa agggtttgat ttgttgcagt acttcaccaa acacatggct tgggatcacc
    15481 acaagaatat tacggctcgc ttcaatcgct ttttgcaaag aagcttccat atataaacta
    15541 tcaggaaagg aaacatcggg cagaaaggct tgattacagc gcgcttgttc taatgccgca
    15601 acgtgcttgg gatcatgccc ccacagcaca acatcgtgcc cattacgcgc taaggtaatc
    15661 gctaaagcgg tgccgtatga accggcaccg ataactgtca tagaagcgtt cattaagctt
    15721 cttcaactcc tgagtttgca gcatcctgtt gctgttgttg ctgtaagtag ttcataaaca
    15781 acgcatcaaa gttaacaggt gctaagttta attgtgggaa agtaccacga cccactaagt
    15841 tggtgataca ttcacgtgca taagggaata atacgttcgg
//
