LOCUS       KY710695               15590 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 31/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710695
VERSION     KY710695.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 15590)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15590)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15590
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 31/57"
                     /serotype="O16"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..15590
                     /note="O antigen gene cluster"
     gene            116..589
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             973..2343
                     /note="orf02"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf02"
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="AXY99472.1"
                     /translation="MPRNIAVILASGSGSRFESKIPKQFVKLAGKPVIQYTIEAFENC
                     SAIDEIYIVTKEDYIDLVYEITNKNELKKVSKVISGGKERYDSTWAAINATPSENCNL
                     IIHDAVRPFISERIINDCIAALVTYNAIDVVVDAVDTIVEVDGDFISNIPDRRRLKRG
                     QTPQAFKRDTLKEAYNKFLLDPQKAASDDCGIVLKYLKNEKIYTVVGAESNFKLTHQQ
                     DLYLADNIIKDGLYTRLTTDNNLIKETIENKVIVVIGGSSGIGEDIVTLCEKLNGKVY
                     SCSRSQNNVDVTDKNSIELFLHEVFLKEKKIDFIVNTTGLLIKKPLLSMSDSEITDSY
                     MINYTGVINVALSSYQYLKETQGMLINFTSSSFTRGRPNYSIYSSTKAAIVNFTQAIA
                     EEWLPLGIKVNCINPERTDTPMRRSNFGIEPPNSLLSAEAVAKFTLSAMSFEHTGQVF
                     SIKNDL"
     CDS             2340..3566
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99473.1"
                     /translation="MSLKKNIINLFGTQIISYLGPLLLLPYLSHILDTNYFGLYIFTL
                     SIINFSSIITNFGFDISIPKKIAEGKNSKDELNTLNYHVNLIKLILSLFTLSGICVVI
                     YYTNYYQNELSSIVLLLLSIFFNSFNFAWLFQGVEKIYIYSRIVIISKLISFLLIFIF
                     VTSNEDFVLIMTIILIQNILSLLISFYIIFEKMNIKIKKSSLNDTWYIAKDSTEYFIS
                     RLGVSLYSTCGSFFLGIFSGSLHQVAIYGAAEQLYRAGVSAMSSISSPLTPFMARTKD
                     FNLLFKVIKLALLLTISGSLFGFIVGDYIINIIYGVNYENAKPVLNVFMITIIFSVIG
                     MILGYPALIPLGKARSANYSVMYAGILQILFIITMLLFEIPFNAINIAISYLLCDIFM
                     FTYRGLIFIKGYKIKK"
     CDS             3563..4360
                     /note="orf04"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf04"
                     /product="LicD-family phosphotransferase"
                     /protein_id="AXY99474.1"
                     /translation="MNKYDLRKKQLKMLKGLIEIHRICQLHNINYWLDAGTLLGAVRH
                     SGFIPWDDDIDICMFRDDYNKFINIAKYELNTNDFFLQTVTTDKDYIFINIPCKLRIN
                     NTEIVEKFEKIHNCYNEKSHHGLFVDIFPYDKYSENKNKRKIQRLLSIAYKIKKLSYC
                     NNIGAIKYILSRIFSIILPIIILEKTKNIIANKMNKEPNNSLYGAGIETPFTRAYFSE
                     DEIFPLKKIFFEGYEFNCPNNTKIYLEKMFGPNYMTPPPEEERYNHS"
     CDS             4383..5306
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99475.1"
                     /translation="MDKLLNIGIEINNNSYTPEFYAYKAFLEKKMHKVQLLNKDELSH
                     NKSFDIKIYTMGFSHKWRNKEETKIIHEYQSLSTGYGNKIKNNIKKYLNIKPNGRIFL
                     NSYVRKTLNFNDNIDYIERDMGIDESFFNITQNNIQKEYDIVYCGSINRYGLIKTLIN
                     LSKLGLKILVIGNVSLKIEKKLKKYNIICTGKIQRNNIPPLYKMATFGLNYTPNIYPF
                     NVQTSTKTLEYCASGLKILSNHYNWISNFEKERNAKFLWLHDLFNIGNIDILKTKENS
                     LITPNVKDLMWDNILKNSKLEEFLYKIYNEE"
     CDS             5309..6676
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99476.1"
                     /translation="MELNKIKKTTLVLIILNFFSFLSSFFLFINTNNYNILSYSYIIN
                     IILTIIIFINEKKRLTSLLFLFNISQYLFIGGRIITFPFLEHELNSPFDLEFMTNFYS
                     STNNSIYVYSIIFLFNFLINLFYFFIKINNNKKNTIWLNILVCPIFFFYILLFLLLSI
                     SEFNLYLKTKDLGYLARYSYQSEQYSSGNSTLLILFYTFLGLLFCFRNKKIIIFSYLL
                     LLIYSFIIILGGQRGPFISAIMMGIWLYGINKNISLKKLMSLFFLLFLFLIIIMGISS
                     RGVNGDINNYVYLLEMFIYKQGVTLGVISYSISDFQNFPIMAYVNSLIPGATRIYSLF
                     IDNQLSLPDLGFGPFVSHTANNVMFQEGYGLGWSILLNLYLFSFNNPLIFIFFCFLFS
                     YFLNKLDNCFYHDYWFGLSGTLATKIVFSPRDDLKNIIHFAIFFTIAYFLLKIINTTL
                     LRYRN"
     CDS             6703..7839
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99477.1"
                     /translation="MKIAIIGTTSASLLNFRKELIISLVSLGHIVYCFSIDYTKESKF
                     EVSQLGAIPIEYKLSRSGLNPFLDIKNTYILYKILKRLNIELTLSYFSKPAIFGTIAA
                     KLANTPQIYAMLEGLGYYFTEQKNGLSLKTKIVKYIQILLYKIAFPLSNGLILLNNDD
                     KKDLIERYKIKTEVFIWGGIGLDLNQYKKSDIIINDKISFLFIGRLLKEKGIFEYIHA
                     AEKIKKSYSNIEFIVLGDIDMDNPGSLTKKDLDNLIEKNIITYPGHVDNVIEWLNKSH
                     VFVLPSYREGLPRSTQEAMAIGRAIITTDVPGCRDTVSNKKNGLLVKPWSSDELASKM
                     EYLIKNKSEIIKMGNNSYSYAQKNFDQKITNKKILNYMKITNEK"
     CDS             7959..9125
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99478.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNQKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLDAFKAMSDVIITNRMATALNDVEEKVYTRDLFGND"
     CDS             complement(9640..11196)
                     /note="orf09"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf09"
                     /product="hypothetical protein"
                     /protein_id="AXY99479.1"
                     /translation="MKNFFISKYTKLIINIIIYVVPIALLLISSKLMTIGVGGESSVN
                     DVVLLTILIIVLSSSRKAFYFIALPIVLLYALYTPIGITFGKPTYQYIASLFATDLLE
                     SGEFLYQIPLINWFCAIAIIILIFSYYFLCRRLNISYYKNRTLICIMVIVSLIDQSPF
                     SFFRNIITSGQKVKEELVKLNSFDKENRWGNTRLTNNSKYDDYILVIGESARKDYHHA
                     YGYPINNTPFMSASNGVLVDGFTSAGTNTIASLRLMLTKPNLKKWEPDYSLNLIDLVK
                     NSGIKTYWISNQGYFGKFDTPISAIANKSDVKIFMKSGTYNSYNISDFELIPRFDKII
                     FGKSKTKRFIVIHLYGSHPMACDRLFDYKKIIEPKDPKYDYLACYISTINKTDDLLKS
                     LVNSLNKNYQDNNRTYSLIYFSDHGQVHNEVNGKILFNNAFAGKRHYDVPIFKISYDD
                     HERNVIKNFKSGLNFIDGIAHWIGIENDKLNIGYDIFTNKDDQNDYGLSDRIKAINKE
                     DDPAIDIKDK"
     CDS             11291..11794
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99480.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(11932..12909)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99481.1"
                     /translation="MKNNMPKITVLMSVYNDEKYLQESIDSILNQTFKEFEFLIINDG
                     STDNSLSILKENAKKDPRIKLIINEQNIGLIASLNKGLQLASCDLVARMDSDDIAVST
                     RLEAQYQFMLQHPEVSVCGGAFIYQEEPNRINTYPETHSDIVISSLYDCSLAHPTIIY
                     RKQDILSVGGYDKADLYAEDYGLWVNLIIAGKFKLHNLPVPLLYYRTHPNVERSQYKA
                     IQKDTAERVGRKLLMYFGINVNKEDYSLLLDNDKKTVEQVVRCKRLLEKINQQFNAKE
                     YRKELIQKNEFKKKLLERKVLVTDFSSFLIKISARWYFLIRKITKKAKK"
     CDS             complement(12919..13476)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemH"
                     /product="wemH"
                     /protein_id="AXY99482.1"
                     /translation="MHSQAKVTLAELKECLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGNHVYIGCNSSIIGGEIKIGDNAIIGAH
                     ALVLKDLGERCRYINKMNFEINKYC"
     CDS             complement(13479..14312)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99483.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPMLASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(14408..14824)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99484.1"
                     /translation="MSDGMGFGANARTALITRGLAEMSRLGKALGADAATFMGMAGLG
                     DLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEKIGQVVEGYRNTKEVRALAEQVGVEMP
                     ITEQIYQILYQHKDVKEAALALLGRATKDEIDSSLL"
     CDS             complement(14817..15425)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA_2"
                     /product="gpsA_2"
                     /protein_id="AXY99485.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKKRDCYWCGYV"
BASE COUNT     5346 a   2529 c   2385 g   5330 t
ORIGIN
        1 acggtgaagt aaagagcttt gagcgtattg aaacagaaac acagcggctg gatggcatga
       61 tcaatgactt attagtgctt tctcgcaacc agtataaaaa tgagttatta cgtgaaaccg
      121 taaagcgaat gagctttgga acgatatttt agataatgca aaatttgagg ctgaacagag
      181 caataaaacc ctaacagtaa ccgccccacc aggcccatgg acaatttatt gcaatcctta
      241 ttcattagcg agtgcctttg aaaatatcgt tcgcaatgcc ttgcgttatt cacattctcg
      301 tattgaagtg gcctttaccg aacaaaatca gggtatcact attattgttg atgatgatgg
      361 cccaggggtt agcccagaag atcgcgagca tattttccga cctttttatc gtacggacga
      421 agcacgtgat agagaatccg gcggtacagg gctgggactg gcaatagttg aaaccgcgat
      481 tagtcaacat cgaggccatg taaaagctga cgatagccca ttaggggggt taagagtgga
      541 gatttggttg ccgagaggaa gtgtggtaaa taaatcacaa cacgcttaat tcagtaataa
      601 aattactata ttaaattatt ctgccatcta ttgaagaata ggtggcatat tcttttcatt
      661 cccatcttta tagatatttc agaatgatct tgctaattca atcgatattc cgatatttat
      721 atatacctaa tcaatgttag aatacccatc aattacttga cccgcgggtt tgaagagtct
      781 aacctcagag cggttttgcg tactgttaaa gtatacttat ataacaaaac tcttatatta
      841 cagatagata agagattgtc gagaaaattt tggcgctctg ggggccgtaa acccagtgcg
      901 gtagctttat ataataagat ttatttttac cctacaatta ataaattaat tattattatt
      961 aaaggataaa taatgcctag aaatattgca gtgatcttag cttcaggaag tggttctcga
     1021 tttgaatcaa aaatcccaaa acaatttgta aaattagcag ggaagcccgt cattcaatat
     1081 actatcgaag cttttgaaaa ttgttcagct atagacgaaa tttatatcgt caccaaagaa
     1141 gattatatcg acttagttta tgaaatcact aataaaaatg aattaaagaa agtttctaaa
     1201 gtgatttctg gtggaaaaga acgttatgat tctacatggg ctgcaattaa tgcaacacca
     1261 agtgaaaatt gtaatctaat cattcacgat gcggttaggc cttttatttc tgaacgcata
     1321 atcaatgatt gtattgcagc tttagttacc tataacgcta tagatgttgt tgtagatgct
     1381 gttgatacga ttgttgaagt tgatggcgat tttatttcta acatccccga tagaagaaga
     1441 ttaaaacgag gacaaactcc ccaagccttt aaaagagata cattaaaaga agcatacaac
     1501 aaatttttgt tagatccaca aaaagctgca tcggatgact gcggtattgt attaaaatat
     1561 ctcaaaaatg aaaaaatata tactgttgtt ggtgctgaat ctaactttaa gttaactcat
     1621 caacaagatt tatacttagc agataatatt attaaagatg gtttatatac tcgtttaact
     1681 accgataata acttaataaa agaaacaata gaaaataaag ttattgttgt tattggaggt
     1741 agtagcggta taggagaaga tatcgttact ctgtgtgaaa aattaaatgg taaagtttac
     1801 tcttgcagta gaagtcaaaa taatgttgat gttactgata aaaatagtat tgaattattt
     1861 cttcatgagg tttttctaaa agaaaagaaa atagatttta ttgttaacac aactggctta
     1921 ttaataaaaa aacctctttt atcaatgagt gatagtgaaa taactgatag ttatatgatt
     1981 aattacactg gtgtaattaa tgttgcactt tcgtcttatc agtatttaaa agaaacccaa
     2041 ggtatgctaa ttaattttac ttccagttca tttactcgag gaagacctaa ttatagcatt
     2101 tactcatcta caaaagctgc tattgttaat tttactcaag ctatagctga agaatggtta
     2161 cctttaggta ttaaagtaaa ctgtattaat cctgaaagga cagatacacc gatgaggcgt
     2221 agtaattttg ggattgaacc acctaattca ttacttagtg cagaggctgt tgcaaaattc
     2281 actctatcag caatgtcatt tgaacataca gggcaagtat ttagcataaa gaatgattta
     2341 tgagccttaa gaaaaatata attaatttat ttggaaccca gataattagt tatttgggtc
     2401 cattattatt actcccttat cttagtcata tattagacac aaactatttt ggactttata
     2461 tttttacttt atctataatt aattttagtt caataataac caactttgga tttgatatat
     2521 ctattcctaa aaaaattgct gaagggaaaa acagtaaaga tgaacttaat accttaaatt
     2581 accatgttaa tttaattaaa ctcatattat cattatttac tctttctggc atatgtgttg
     2641 ttatatatta tactaattac tatcaaaatg aattatcttc tatagtacta ttactgttat
     2701 ctattttttt taattcattc aattttgctt ggttatttca aggtgttgaa aaaatatata
     2761 tatatagccg tatagtgata attagtaagt taatttcttt tttacttatt tttatttttg
     2821 ttacatcaaa tgaagacttt gtactaataa tgaccataat attaatccaa aatatattat
     2881 cattattaat ttctttttat atcatctttg aaaaaatgaa tataaaaata aaaaaatcta
     2941 gcttaaatga tacatggtat atcgcaaaag attcaactga atattttata tctagattag
     3001 gagtatcatt atactcaact tgtggtagtt ttttccttgg aatattctca ggatcgctac
     3061 atcaagtagc catatacggt gcagcagaac aactatatag ggctggagtt tctgctatgt
     3121 catccatatc atcaccgctt acaccattta tggctagaac taaagacttt aatctattat
     3181 ttaaagtaat aaaattagca ttattattaa ctatttccgg ttctttattc ggttttattg
     3241 ttggtgatta tatcataaat ataatatacg gagttaatta tgagaatgca aaaccagttc
     3301 ttaatgtgtt catgattact attatattta gtgtcatcgg gatgatatta ggttatccag
     3361 ctttaattcc attaggaaaa gctcgaagtg ctaattatag tgtcatgtat gctggaatat
     3421 tgcagatact gtttataata actatgttac tttttgagat cccatttaat gctattaata
     3481 tcgctatttc atatttatta tgtgatattt ttatgtttac ttatcgtgga ttaatattta
     3541 ttaaaggata taaaattaag aaatgaacaa atatgattta agaaaaaaac aattaaaaat
     3601 gctaaaggga ctaattgaaa ttcacagaat atgtcagcta cataatataa attattggtt
     3661 agatgctgga actttacttg gagctgttag acatagtggc tttataccat gggatgatga
     3721 tattgatata tgtatgttta gagatgacta taataaattt ataaatattg caaaatatga
     3781 attgaacact aatgattttt ttttacaaac agtaacaact gacaaagatt atatttttat
     3841 aaacattcca tgtaaattaa gaataaataa tacagaaata gttgaaaaat ttgaaaaaat
     3901 acataattgc tataatgaaa aatctcatca tggtttattt gttgatattt ttccttatga
     3961 taaatattct gagaataaaa ataaaagaaa aattcaacgg ctattgtcaa tagcatataa
     4021 aataaaaaaa ctatcttatt gtaataatat tggagcaata aaatatattc taagccgtat
     4081 tttttctatt attttaccta ttattatttt agaaaaaacg aaaaatatta ttgctaataa
     4141 aatgaataaa gaacctaaca atagtttata cggtgctggc attgaaactc ctttcactag
     4201 agcatatttt tctgaagatg aaatatttcc attaaaaaaa attttttttg agggttatga
     4261 atttaactgc cctaataata ctaaaatata tttagaaaaa atgtttgggc caaattatat
     4321 gacacctcca cctgaagaag aaagatataa ccattcctaa cttattaaat ggaatataaa
     4381 taatggataa gttgttaaat ataggtatag agataaacaa taattcttat acacctgaat
     4441 tttatgctta taaagctttt ctagaaaaga aaatgcataa agtgcaatta ttaaataaag
     4501 atgagctcag tcataataaa tcttttgata taaaaattta tactatgggg ttctctcata
     4561 aatggagaaa taaagaggaa acaaaaataa tacatgaata tcaaagtctt tcgactggtt
     4621 atggaaataa aataaaaaat aatataaaaa agtatttaaa cataaaaccc aatggtagaa
     4681 tttttctaaa ttcttatgta agaaaaacct taaacttcaa cgataatatt gattatattg
     4741 aacgagatat gggaatagat gagtcgttct ttaatataac tcaaaataat attcaaaaag
     4801 aatatgatat agtctattgt ggttcaatta atagatatgg attaattaaa acattaatta
     4861 atttatcaaa gttaggtcta aaaatattag taatcggcaa tgttagttta aaaatagaaa
     4921 aaaagttaaa aaaatataat attatttgca cagggaaaat acaaagaaat aatattccac
     4981 ctctctataa aatggcaact tttggattga attatacccc caatatatat ccatttaatg
     5041 ttcaaactag tactaaaaca ttagaatatt gcgcatcagg tttaaaaata ttatctaatc
     5101 attataactg gatatcaaat tttgagaaag aaagaaatgc aaaattctta tggcttcatg
     5161 atttgtttaa tataggtaat attgatattt taaaaacaaa agaaaactca ttaataacgc
     5221 ctaatgtaaa agatcttatg tgggataaca tattaaaaaa ttctaaatta gaagagttcc
     5281 tttataaaat atacaatgag gaataaaaat ggaactaaac aaaataaaaa aaaccacatt
     5341 ggttttaata atattaaatt tttttagctt cttaagctcg ttttttttat ttattaatac
     5401 taataattat aatattcttt catatagcta cataataaac ataattttaa caatcattat
     5461 ttttataaac gaaaaaaaga gattaacatc tttattgttt ttatttaaca ttagccaata
     5521 cttatttatt ggtggaagaa taattacttt tccattttta gaacatgaac tcaattcacc
     5581 ttttgaccta gagtttatga ctaattttta tagctctacc aataactcaa tttatgtata
     5641 ttcgatcatt tttttattta attttttaat taacttattt tattttttta ttaaaataaa
     5701 taataataag aaaaacacaa tctggttaaa cattttggta tgcccaattt tctttttcta
     5761 tatactatta tttttattac tttccatatc agaatttaat ttatatctta aaacaaaaga
     5821 tcttggttat ctcgctagat actcatatca atcagaacaa tatagctcag gtaacagtac
     5881 tttattaatt ttattttata catttttagg tcttctattt tgttttagaa ataaaaaaat
     5941 aataattttt tcttatttat tattattgat ttatagtttt ataattatat taggaggcca
     6001 aagagggcct tttatttcag ctataatgat gggtatttgg ttatatggta taaataaaaa
     6061 tatctctctt aagaaactta tgtctctatt ttttttatta ttcttatttt taataataat
     6121 aatgggtatt tcttcaagag gagtgaatgg agatattaat aactatgtat acttgttaga
     6181 aatgtttata tataaacaag gggttactct tggtgttata tcatatagta taagtgattt
     6241 tcagaatttt ccaattatgg cctatgtaaa ttcactaatt ccaggggcga ctagaattta
     6301 ttcgcttttt attgataatc aacttagctt accagatctt ggttttggac cttttgtctc
     6361 tcacacagct aataatgtta tgtttcaaga aggatacgga ttgggatgga gtattttatt
     6421 aaatctatat ctgtttagtt ttaataaccc attaatattt atattttttt gttttctatt
     6481 ttcttatttt ctcaataaac tagataattg cttctatcat gattattggt ttggattatc
     6541 agggactctt gcaacaaaaa tagttttttc accaagagat gatttaaaaa atataattca
     6601 ttttgcaata ttttttacaa ttgcatattt cttattaaaa ataataaaca caacgctatt
     6661 aagatataga aattaattta tacattatat agaatataaa tcatgaaaat tgcaataatc
     6721 ggtaccactt ctgcttcatt actaaacttt agaaaagagt taattattag ccttgtatcc
     6781 ttagggcata ttgtatattg tttttcaatt gattacacta aagaatcaaa atttgaagtc
     6841 tctcaattag gtgctattcc tatagaatat aagctaagta gatctgggtt aaatcctttt
     6901 ttagatatta aaaataccta tatactatat aaaatattaa aaagacttaa tatagaactt
     6961 actctttctt atttttctaa accagcaata tttggtacaa ttgccgcaaa attagctaat
     7021 actcctcaaa tatatgcaat gctagaagga ctgggatatt attttactga acaaaaaaat
     7081 ggactaagtc taaaaacaaa aatagttaaa tatattcaaa tattattata taaaatagca
     7141 tttcctttat caaatgggtt gattctacta aataatgatg acaagaagga tttaattgaa
     7201 cgctataaaa ttaaaacgga ggtatttatt tgggggggga ttggattaga ccttaatcaa
     7261 tataagaaat cagatataat tattaatgat aaaatttcct ttttatttat aggtaggcta
     7321 ttaaaagaaa aaggaatatt tgagtatatt catgcggccg aaaaaataaa aaaatcatat
     7381 tctaatattg aatttatagt attaggtgat atagacatgg ataacccagg aagtcttaca
     7441 aaaaaggact tggacaattt aatagaaaaa aatattataa cctatcctgg acatgtggat
     7501 aatgtcatag agtggttaaa taaaagtcat gttttcgtac taccgtcata tagagaagga
     7561 ttacctagaa gtacacaaga agcaatggca ataggtcgag ccatcattac tactgacgtt
     7621 ccagggtgtc gagacactgt ttctaataaa aaaaatggat tgcttgttaa accatggtca
     7681 tctgatgaac ttgcatctaa aatggaatat ctaattaaga ataaatctga aataataaaa
     7741 atgggtaata atagctatag ttatgctcaa aaaaatttcg accaaaagat aacaaataaa
     7801 aaaattttaa attatatgaa aatcacaaac gaaaaataaa attaattgat gatatacata
     7861 tacacctgat agatttaact ataattaata ataattttat ttaatcttat acatctattt
     7921 tttagaattt atatacacaa ttcatagcag agaaacacat gaaaatcacc atctcaggaa
     7981 ccggctatgt cggtctatct aatgggatac tcctttccca gcacaacaaa gttgttgcat
     8041 tagatatcaa taaagaaaaa gtcgaactat taaaccaaaa aatctctcct atctctgata
     8101 aagaaattga agagttttta gccactaaag agctcgactt tacagcaact ttagataaag
     8161 aattcgctta taaagatgct gaattcgtta tcattgccac tcctaccgat tatgatccta
     8221 aaactaacta ctttaacacc tcttctgtag agtcggttat tcgtgatgtt ttagaatata
     8281 atccaaacac cactatgatt ataaaatcaa ccattccagt tggttttact aaacagatga
     8341 gagaaaaata tcataccgat aatattatct tctctcctga gttcttacga gaaggtcgcg
     8401 ccctttacga taacctttat ccatctcgta ttgtgattgg tgagcaatct gcaagagcag
     8461 agaaatttgc tgagctttta caacaagggg cattaaaaaa agatattgat gttcttttta
     8521 ctgactctac agaagcagaa gcaatcaaac tatttgccaa tacttatctc gcactgcgtg
     8581 ttgcttattt taatgaactc gatagctacg cccaagctta cggcttaaat gcaagacaaa
     8641 ttattgaagg tgtttgttta gatccgcgta ttggtaatca ctataacaac ccatcatttg
     8701 gttatggtgg ctactgctta ccaaaagata cgaaacaact gttagccaac tacgaatcag
     8761 taccgaataa tattattggt gccattgtgg aagctaaccg cacccgtaaa gactttattg
     8821 ctgattccat tatcgctaaa tcaccgaaga ttgttggcgt ttaccgttta attatgaaat
     8881 caggctctga taatttccgc tcatcttcaa ttcaaggcat tatgaagcgt attaaagcaa
     8941 aaggcattga agtggtaatt tatgagccag aaatgaaaga agagactttc tttaactcaa
     9001 aactcattcg tgatttagat gcgtttaaag ccatgtctga tgtcattatt accaatagaa
     9061 tggctacggc gctaaacgat gtggaagaaa aagtttatac tcgtgatctc tttggtaacg
     9121 attgaaaaat taatagatat ttctttgcat aattagattt ttcatcgagt aggagacggg
     9181 attactcccg tcgtcctctc actccaccgt atgtacgatt ccgtatacgg cggttcatgt
     9241 taactctgga actgcctata ttgctctaat agtgataaca aacctgcttt ccttaacaac
     9301 attccggctt ttattgcctg atgcatgtgg cttgctcccg agttccacca tgcccctcgt
     9361 tgatttggtc gctgatcacc aagctatttc ttcaaataac cccgcacgca tcaacttctt
     9421 cttcgcacgc gcgacgggcg tttccattgt cgccaaagca agcaacgcaa ctttctgttt
     9481 atccagttat ctaatttctc cagttcctct tttactgcgg tgtatctaaa gtagggagtg
     9541 gacaaggcgg actatataaa aagccattta ttattaaata aatggctata acattttatt
     9601 tcaaatactc aaagttcaat attgattgta ctaaataatc tatttatcct ttatatctat
     9661 cgcaggatca tcttctttat taattgcttt tattctatcc gataacccat aatcattttg
     9721 gtcatcttta ttagtaaaaa tatcatatcc tatatttaat ttatcattct caataccaat
     9781 ccaatgagca atcccatcga taaaatttaa gcctgatttg aaatttttaa taacatttct
     9841 ttcgtgatca tcataagata ttttgaaaat aggtacatca tagtgccgtt ttccagcgaa
     9901 cgcattatta aataatattt ttccatttac ttcattatga acctgaccat gatctgaaaa
     9961 ataaattaat gagtatgttc gattgttatc ttgatagttt ttattcaaag aatttactaa
    10021 tgactttaac aaatcatctg ttttatttat tgttgaaata taacatgcta aataatcata
    10081 tttaggatct ttaggctcta tgattttttt ataatcaaat aatctatcgc aagccatagg
    10141 atgcgaacca tataaatgaa ttacaataaa acgtttagtt ttactctttc caaaaataat
    10201 cttatcaaac cgaggaatta attcaaaatc actgatatta tatgaattat aagtcccaga
    10261 tttcataaaa atctttacat cactcttatt agcaattgca gaaattggag tatcaaactt
    10321 accaaaatat ccttgattag aaatccaata tgttttaatc ccactgtttt ttactaaatc
    10381 aattaaatta aggctataat caggttccca tttttttaga ttaggttttg ttagcattaa
    10441 tcgtaacgat gcaatagtat ttgtccctgc agatgtaaaa ccatctacta atacgccatt
    10501 tgacgcactc ataaatggag tattatttat aggataacca taagcatgat gataatcttt
    10561 tctagcactt tcacctataa ctaaaatata atcatcatat tttgaattgt tagttaatct
    10621 tgtattaccc catctatttt ctttatcaaa agaatttaat ttaactaatt cttcttttac
    10681 tttctgacca gaggtaataa tatttctaaa aaaagagaat ggagattggt ctattaaact
    10741 aacaattacc atgatacaaa ttaaagttct atttttatag taggaaatat tcaaccttct
    10801 acataaaaaa tagtatgaaa aaattaatat aatgatagct atcgcacaaa accaatttat
    10861 tagtggtatt tggtataaaa attcaccact ttctaacaag tcagttgcaa ataacgatgc
    10921 tatatattga taagtgggtt tgccaaatgt aatacctatt ggtgtatata aagcgtataa
    10981 taaaactatc ggtaaagcga taaaataaaa tgcttttctt gacgaactta atactattat
    11041 tagaatagtt aatagtacta catcatttac actactttca cctcctactc caatcgtcat
    11101 tagtttcgat gagattagta acaacgcaat tggtactaca taaattatta tattaattat
    11161 tagtttggta tattttgata taaaaaaatt tttcatatta ttatgtaatt cttagtttat
    11221 catattttga catgaataat tattatataa tattatatga taattatttt ctaaatcata
    11281 gatattttat atgctcaata tcgtcttatt tgaacccgaa attccaccga atacgggtaa
    11341 tattatccgc ctgtgtgcta acacaggctt tcatcttcat ttgatccaac cattgggttt
    11401 tacttgggat gataaacggc tacgccgtgc agggcttgat tatcgtgagt ttgctgatat
    11461 caaacagcat cacgattatt atgctttttt agaaagtgaa ggattaaacc ctgataatgc
    11521 acaatccccg acaggtgcac gattgtttgc attaaccact aaaggtaccc ccgcccatag
    11581 taatgtaagt tatcaagatg gggattatct gctctttggc cctgagacac gaggtttacc
    11641 accttatgtg ttggataata tgccaacaca acaaaaaatc cgtgtgccaa tgttagcgga
    11701 tagccgtagt atgaatcttt ctaatacggt tgccgtagtt gtttttgagg catggcgcca
    11761 attgggctac cctggtgcgt tattaagaga ttgatttatc tttttaattt ataaccataa
    11821 aaaatgacct ctagacttta ttgtctcgag gtcattttta tgtatatgct ttgtgctagt
    11881 tttagctttt cttattttat atcttctttt tatctttttt atctttcggt tttatttctt
    11941 agctttttta gttatttttc ttatcaagaa ataccatctt gcacttattt ttattaaaaa
    12001 tgatgagaaa tcagttacta atacctttct ttctaataat tttttcttaa attcattttt
    12061 ttgtattaac tcttttcgat actctttagc attaaattgt tgattaatct tttcaagtaa
    12121 ccttttacag cgtactactt gctctactgt ttttttatca ttatccaata ataatgaata
    12181 atcctcttta ttcacgttaa tgccaaaata cattaataat tttcgtccta ctctttccgc
    12241 ggtatctttt tgtatcgctt tatattgcga acgttcgaca tttggatgag tacgataata
    12301 aagaagtggt acaggaagat tatggagctt aaattttcca gcaataatta aattcaccca
    12361 taaaccataa tcttctgcat agaggtctgc tttatcatat ccaccaacag ataatatatc
    12421 ttgcttacga tagataatgg ttggatgcgc taaggagcaa tcataaagac tagaaatcac
    12481 aatatcacta tgtgtttctg gataagtatt aattctattt ggctcttctt ggtagataaa
    12541 agcaccacca caaacactca cttcaggatg ttgtaacata aattgatatt gcgcttctag
    12601 tctggttgaa acggcaatat catctgagtc cattctagcg actaaatcac atgaagctaa
    12661 ttgtaatcct ttgtttaaag aagcgatcaa tccgatattt tgttcattaa taatcagttt
    12721 tattctggga tcttttttgg cgttttcttt taagatagat aatgagttat ccgttgagcc
    12781 atcattaatg ataagaaact caaattcttt aaatgtttga tttaatatag aatcaataga
    12841 ctcctgaagg tatttctcat cattgtatac cgacattaat acggtaattt tgggcatatt
    12901 gtttttcatt atctattttc agcaatattt attaatttca aaattcatct tattgatata
    12961 ccgacacctt tcccccaaat cctttaatac taatgcatgg gcgccaataa tcgcattatc
    13021 accaatctta atctctccac caataatgga tgaattacag ccaatataaa catggtttcc
    13081 tacttggata ttggcttttc cccctttctc ttttacgcca atagtcactc cttgcataat
    13141 agtgacatat tcccccagtt ggcagtaatt agtcagtaca atgccaatcg ggtgtggtaa
    13201 agaaaggcct ttaccaatac gagtttttaa cgagatatca ttacagtatt tacggttatt
    13261 gctagcaccg atccgaatac ctaatttatg taaaaagcga tggggagaga aataaaaata
    13321 ctgtgctacc cgccaccaaa ataggtaacg ggaacgctga cagaaaaata ctcgatgaaa
    13381 tagtcttaac caactaaagg gttttttatc accaatatat tcagcctgta agcactcttt
    13441 tagttcagca agggtaacct tagcttggga atgcatagtt aaataccatc accacactca
    13501 aagccgggta caacaccatt aaagtgttga tccatatcga atgacggttt ttcactatca
    13561 ggacgaccga caatgcgggc aggcaccccc gccaccgtag tatgagccgg tacttcatgt
    13621 aatactaccg agccagcgcc aattttggca ccacgaccaa tctcgatatt accaagaatt
    13681 ttagctcctg cgccaatcat taccccttca cgcactttcg ggtgacgatc gcctcctgtt
    13741 ttacctgttc cccctaaggt cacagattgt aaaatggaga catcattttc aaccaccgcc
    13801 gtttcaccaa tcacaatacc tgtcgcatgg tcaagcataa taccgtgtcc aatacgtgcc
    13861 gctgggtgga tatccacccc aaaggtgact gatatttggt tttgtagata aatagctagc
    13921 gcttggcgat tttgttgcca tagccaatgg gcaatacggt aggcttgtag tgcatgaaac
    13981 ccttttaaat agagtaatgg ggttgaatat ttatctaccg cgggatcacg taggcgtacc
    14041 gcggagagat cacaagcggc cgactcaatc attgagctat cgtgactata agcctcttca
    14101 acgatttcgc gtactgcaat ggctggcata gtctgagttg ccagcttatt ggccagcata
    14161 tagcttaatg cactacctaa attttcgtgt ttgagaagtg tcgcatgaaa gaaactggct
    14221 aacattggct cacaatctgc caatgcacgc gcttcgcttt taatatgatc ccagactctt
    14281 gttagttctt ctagcgacat actcactctc attttttaca cgaaagccta agtaaatact
    14341 taggctttga attttcaaac aaatggatag taaaacagat accctaagaa tattatttaa
    14401 aataaactta caacaggctg ctgtctatct catctttggt tgctcgccct aataaagcca
    14461 atgcagcctc ttttacatct ttatgttgat ataaaatttg gtaaatctgt tcggtgattg
    14521 gcatctctac acccacttgt tcggctaatg cgcgaacttc tttggtgtta cgataacctt
    14581 cgactacctg gccaattttc tcttgggcag tatcaacatc taaaccttga cctagcatca
    14641 taccaaagcg acgattacgt gattgattgt ctgtacaggt taacaccaaa tcacccaaac
    14701 cagccatgcc cataaaagtt gccgcatctg cacctaaggc tttacctaag cggctcattt
    14761 cggccagacc acgggtaata agtgcggtac gcgcattagc gccaaatccc ataccatcag
    14821 acatacccgc accaatagca atcacgtttt ttaacagcgc cccccagttg cacaccgata
    14881 aaatcagggt ttttatagac tcggaagctt ttaccacaat gaaatagctg ttgtaactgt
    14941 tctaaaaaga gattgtctgt cgaggccact gcaatcgcgg tgggcaaacc cgccgcaagc
    15001 tctttagcaa aagtagggcc agataatact gcgagcggga tttcattacc taatacttca
    15061 cgggcaacat cttgtaataa gcgaccagta tgtgcttcaa gaccttttgt cgcccaaaca
    15121 acacgtgcat cctgacgtaa aaagggtttg atttgttgca gtacttcacc aaacacatgg
    15181 cttgggatca ccacaagaat attacggctc gcttcaatcg ctttttgcaa agaagcttcc
    15241 atatataaac tatcaggaaa ggaaacatcg ggcagaaagg cttgattaca gcgcgcttgt
    15301 tctaatgccg caacgtgctt gggatcatgc ccccacagca caacatcgtg cccattacgc
    15361 gctaaggtaa tcgctaaagc ggtgccgtat gaaccggcac cgataactgt catagaagcg
    15421 ttcattaagc ttcttcaact cctgagtttg ctgcatcctg ttgctgttgt tgctgtaagt
    15481 agttcataaa caacgcatca agttaacagg tgctaagttt aattgtggga aagtaccacg
    15541 acctactaag ttggtgatac attcacgtgc ataaggaaat agtacgttcg
//
