LOCUS       KY710696               15606 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus vulgaris strain CCUG 4652 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710696
VERSION     KY710696.1
KEYWORDS    .
SOURCE      Proteus vulgaris
  ORGANISM  Proteus vulgaris
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 15606)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15606)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15606
                     /organism="Proteus vulgaris"
                     /mol_type="genomic DNA"
                     /strain="CCUG 4652"
                     /serotype="O17"
                     /culture_collection="CCUG:4652"
                     /db_xref="taxon:585"
                     /collected_by="Cultures Collection, University of
                     Goeteborg, Sweden"
     misc_feature    1..15606
                     /note="O antigen gene cluster"
     gene            3..596
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             960..2009
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99486.1"
                     /translation="MIKKSVLIYGEYSGYGKSLADGFKQLGYKSEVFSFSGDGFKKIN
                     SGITLPEKNKIQRYISIIKLIPTFLKFKNIIIINPEFVSRKNLGFLILSIFKLTRKRI
                     ILMACGDDTPFIKYGLENIIPNWPYSDIPLPQKDYFLKEKDIFTNNLIASLAFKIIPV
                     MYDYRVSWEQTKFKNKLTKTIPLACDGELQPIKKTKDKIVIMHGINRIDFKGTKVVKS
                     ALNEIEKKYPNDVEIIYPEHLPLTEYLEIMKRVDIAIDQTKGNSYGMNAIYSMFSGHV
                     VLAPANDLFLNDINLQDSPIVKIKNTKESIVNSLSLLIEAKNMLDDKKKASQEYAVNV
                     HSPVKIAEKIEHYLI"
     CDS             2022..2903
                     /note="glucose-1-phosphate-thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlA"
                     /product="rmlA"
                     /protein_id="AXY99487.1"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDQDGFQRLLNDGSQFGISLQYAIQPKPEGLAQAFIIGEEFIGSDSV
                     CLVLGDNIFWGQGFSPKLINAANKKNGATVFGYEVQDPERFGVVEFDNNLNAISIEEK
                     PEKPKSNFAVTGLYFYDNTVINIAKNIKPSERGELEITTINQVYLQKNELSVELLGRG
                     FAWLDTGTHDSLLDAGTFVETIEKRQGFKIACLEEIAYNQKWLTEEHIQKAAILYKKN
                     SYGQYLFKLIKKENENN"
     CDS             2890..3282
                     /note="dTDP-6-deoxy-3,4-keto-hexulose isomerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="qdtA"
                     /product="qdtA"
                     /protein_id="AXY99488.1"
                     /translation="MKIINTIYFNKLGDERGSLTSLEQNKNIPFEIKRIYYIFDTKKG
                     ISRGFHAHKKLQQLAICVRGSCRFIMDDGKNREEIILNDPNLGLLINPMQWHEMHDFS
                     EDCIVVVLANDYYDENDYIRNYDSFINS"
     CDS             3294..3815
                     /note="orf05"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf05"
                     /product="butyryltransferase"
                     /protein_id="AXY99489.1"
                     /translation="MILKSKTIKLRFVNENDAEFILSLRTNNTYNRFLSHVDNDILQQ
                     KKWIEQYKKKEKNNEEFYFIIERLDNTPCGTVRIYDIQDNSFSWGSWILNENKTRTAA
                     IESALLVYIFGFEKKYFTHCHFEVMKGNEKVISFHEKFNARKVDEDEHHYFYEIEPKN
                     IIDIKNKFINFIK"
     CDS             3823..4941
                     /note="DegT/DnrJ/EryC1/StrS aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="qdtB"
                     /product="qdtB"
                     /protein_id="AXY99490.1"
                     /translation="MVPFLDLKKINEQYTEELKNACSRVIDSGWYITGKELENFENEF
                     SNYCGTMYAIGVANGLDALTLVLRAWKEQGKLQAGDEVIIQANTYIASILAITENDLV
                     PVFVEPDDKTYNLSVDNIKSAITNKTKVILPVHLYGQINPMKEIMAIAKKNNLLVLED
                     CAQSHGASIDGIKAGNWGDAAGFSFYPGKNLGALGDAGAITTNDKELADTVMALRNYG
                     SHKKYENLYQGVNSRLDEIQAAMLSVKLKYLDQETKIRQEIALKYLENIKNPLIQLPT
                     TAEEYHFNKQSHVWHLFVISTKHRDLLQKYLMQHNIQTLIHYPTPPHKQLAYKQYNSI
                     NLPITEEIHENVISLPFSPVLNTTDIDYIINTINEFKV"
     CDS             4965..6188
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99491.1"
                     /translation="MTGLLTLLRMSMGFVIAKVVAIYTGPTGMAMLGQVQSMVTSLNG
                     IINAPVGSGIVRFTAEHKEKGFDICSIWWRASLYWVALIASFIIPIGIFLSSYIADWL
                     LHDPTLAWIVIITVSVLPFSAIGTICNSIINGQQLYRRFVGLGMLSTLISSSVMLTLI
                     VTSNIEGALLAAAIQSAIIGIVMFIANLRQPWMKFRYWWGNQDYKARKSIAGYMLMAL
                     TTATTVPISLILIRNILIYQIGWDAAGYWQAVWKISEVYLGVITMALGTYYLPKLSAL
                     KSADEIIIEIHKTAKIILPIVIIMSVCVYLLRDVAIWLLFTEEFKPIRDLFFIQLCGD
                     IVKIASWLYAYPMLARGTAKWFIFTEIIFSFSFVIFSYFLVTNLGLEGVTYAYLFNYI
                     IYFLFMFFNIKKIIR"
     CDS             6225..7568
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="polymerase"
                     /product="wzy"
                     /protein_id="AXY99492.1"
                     /translation="MNYNEFLDIYFSNLSLFLTCGFISIILCFYSVNFISIAGITDPI
                     HFFWTYTLGTTYSIIIGLFILGYISNDIIILIGISIVSLLLSIYIGYAFKINLLKHPI
                     NFIIEYNNGYRLFKILLFLMCAFYTVIIYKVGFSIFSEVNRFEQNKGIGPLVRVVDAI
                     RPFVLSYMAIYIGVYTKGYKKIIYNSILISLIIVSSLSNGAKFALLEIVYAISACFIL
                     YRGHRLKVSMKRLIKIFSIFLVVFSFALIATNYAIKAKGENMKPQFLPENTPIAIEKI
                     YLRTLSNGDQAYLGLPNEIINKIPTGNIIYNFTSSMIGKEKFKNIFGINDNLNNVGQK
                     ILLFHFPDYDQAGGPVSHFDLYFYHYLPIGLNYIAIFVLGLVLVSIVNCKVLAKGNIF
                     ISALISTLWIRGLVMILEPAMGFVYILDFLFIIFFITLITIILPKKRKKDFINVK"
     CDS             7558..8478
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99493.1"
                     /translation="MLSKENKPLVSFILVSYNQENYIEEAINSVFNQSYENLQIIISD
                     DGSIDHTKNIILEKIKNRDVLFLNNKENKGLIGNLNLAFSYAEGDIIIAMAGDDISMP
                     NRVTKIVESFIKNPDVFCIYSNTIDIDKDSVEIPNSSYGLKYHHKNGEMTIERHLKEN
                     LGILGCSAAFKRCLVTSPLAHFLPSEDKVLTLRALIKGKILFIPEKLVKYRLGTGISN
                     NLNKKNKFEYKKNLKARILTIDGYILELNNNSMFLNEIKDLKIIKDFLTSALNIVEAK
                     KISLPKHFFNLKSIDFKDKLRFFYYRFYRD"
     CDS             8485..9345
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99494.1"
                     /translation="MLSKLKMLISSIYYTFLFSIRNIFLIRTGKSNSIIKKHPIIVTL
                     TTYPKRISTVFLTIESIFSQTISPEKIVLWLYKEDKPLIPSSLRRLQKRGLEIYFCDE
                     DLRSYKKLSYTNNFFNTHIIVTADDDILYPKNWLEELYNESLKYPNTVICHRGHDISI
                     SNNEIESYSTFMKNKVYSTNPSPLLIPTGCSGILYPIGSLSIDASYKKSSIFMQLAPD
                     ADDFWYKFVCLKNGFLAKRINSENKHFPPIISSLKDGLFHNNVRNNQNDKKLLSTINY
                     FELYDFIIKK"
     CDS             9394..10560
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99495.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNNVVALDINKEKVELLNKKISP
                     ITDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMRKKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFADSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNAKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSRLVRDLNEFKSISDVIITNRMSSVLNDVEEKVYTRDLFGND"
     CDS             10595..11386
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXY99496.1"
                     /translation="MLSKNTNFLISIIMPSYNSGEYIRKSIDSVLRQSYKNLELIIID
                     DCSNDNTIEVISSYKDERIKILKNESNQGAGITRNKGLSLANGRFICFLDSDDYWRDD
                     KLEKQLNFMLSNNIDFCFSAYDRVSEDDAFICKIGVPEKVNYSDMLKVCSIGCLTVMF
                     DKDKFTDFSMPSIRKRQDYALWLKLLKQTPYAYGISESLGSYNIRENSISSNKLDAAK
                     HQWRIYRDIENLNIFSASYYFSHYAFNGFFRSKLPSLAKKLGLLK"
     CDS             11419..11922
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99497.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNTQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(12008..12979)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt5"
                     /product="glycosyltransferase"
                     /protein_id="AXY99498.1"
                     /translation="MKNIMPKITVLMSVYNDEKYLQESIDSILNQTFTEFEFLIINDG
                     STDNSFNILKENAEKDPRIKVVINEENIGLIASLNKGLKLASCDLIARMDSDDIAIDS
                     RLEEQYQFMVDNPNISVCGGGFIYQEDPDRINIYPETHTDIVISSLYDCSLAHPTVIY
                     RKKDILSIGGYDKEDLYAEDYGLWVNLIITERFKLHNLPIPLLYYRTHPTVNRSRYKE
                     IQKNTAERLGRKLLKHFGIRINEESYSLLLDNNKKTKEQVVRCKALLEKIDQQFNAEE
                     YSEELKLKNSFKKKILERKVLVNDFSSFLMKLRARWYFLMRKFNKKK"
     CDS             complement(12990..13544)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenH"
                     /product="wenH"
                     /protein_id="AXY99499.1"
                     /translation="MALTEVTLSELKACLHAEYIGDNKPFSWVRLFHRVFFCQRSRYL
                     FWWRVAQFFYFSKSRFLKKLGIRIGAKNNRKYCNDISLRTRIGKGLSLAHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKDGKADIRVGNHVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYINKMSFEINKYC"
     CDS             complement(13544..14377)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99500.1"
                     /translation="MRVSMSLEELKRVWDHIKDEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDNSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWGQDRKALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAIVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(14455..15471)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99501.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGFDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     SNLF"
     gene            complement(15471..15605)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5396 a   2516 c   2528 g   5166 t
ORIGIN
        1 gtcgtagtgg agaaagtaaa gaactagaac gcattgaaac agaaactcaa cgccttgatg
       61 gcatgattaa tgatttacta gttctttcac gcaaccaaca taaaaacgag ttattacgcg
      121 aaacggtaaa agccaacgag ctgtgggatg acattttaga taacgctaaa tttgaggctg
      181 aacagagtaa taaaacgcta caagtgacaa cgcctccagg agcatggcct atctactgca
      241 atccatattc acttgctagt gcatttgaaa atattgttcg taatgcccta cgttattcac
      301 attctcgcat tgaggttgca tttactgaac aaaaccaagg tatcacaatt attgttgatg
      361 atgatggccc aggtgttagt cctgaagata gagagcatat cttccgacct ttctatcgca
      421 ctgacgaagc aagagatcgc gaatcaggtg gtactggctt gggattagct attgttgaga
      481 cagcagtaag ccaacataga ggccatgtta aagccgatga cagcccatta ggtggattga
      541 gagttgagat ttggttgccg agggctggag ctggtaataa atcaccaaca gcttaataga
      601 gtaattaaac tgttgtatca aaatatactg ccatctattg aagaataggt ggcatattct
      661 ttttgttagc gtttgttgat gcaattcaga atgatctggc tgaatcgacc gctattccag
      721 tatttatata tcttcaatca atgctaaaat actcagctat tacttgaccg tctggtttga
      781 agagtctact ctcagggcgg ttttttagca tattgttaaa gtatgcttat ataataaagc
      841 tcttatatta caatcagata ggagcttttc gagataagct ttggcgctct gggggccgta
      901 aacctagtgc ggtagctatg ataattttat agcataataa aaaattagaa ataacttata
      961 tgataaaaaa atccgttctt atctatggtg aatatagtgg ctatggaaaa tcgttagcag
     1021 atggcttcaa acaattgggt tacaaatcag aagtatttag tttctcaggt gatggcttta
     1081 aaaaaataaa ttcagggata actcttcctg aaaaaaataa aatccaaaga tatatctcaa
     1141 taataaaatt aatccctact tttttaaaat ttaaaaatat aataattatt aatcctgagt
     1201 ttgtaagtcg gaaaaattta gggtttttaa ttctatctat tttcaaacta acaagaaagc
     1261 gaattatctt aatggcttgt ggagacgata ctccctttat aaaatatggt ttagaaaata
     1321 ttattcctaa ttggccatat tcagatatcc cacttccaca aaaagattat tttttaaaag
     1381 aaaaagatat ttttacaaat aatttaattg catccttagc tttcaagata ataccggtta
     1441 tgtatgatta tagagtaagc tgggaacaaa ccaaatttaa aaataaatta acaaaaacaa
     1501 tccctctagc ttgtgatggt gaattacaac caataaagaa aactaaagat aaaattgtta
     1561 ttatgcatgg tataaatcgc attgatttta aaggaacaaa agtagttaaa tcagcactta
     1621 atgaaataga aaaaaaatac cctaatgatg ttgaaattat ctatcctgaa catttacctt
     1681 taactgaata tttagaaata atgaagcgag tagatattgc aatagaccaa acaaaaggaa
     1741 attcctatgg aatgaatgct atttatagca tgttttctgg tcatgttgtt ttagctcctg
     1801 caaatgattt attccttaat gacataaacc tccaagattc tccaattgtt aaaatcaaga
     1861 ataccaaaga gagtattgtt aactcattaa gcttattaat agaagcaaaa aacatgcttg
     1921 atgataaaaa gaaagcatct caagagtatg cggttaacgt acactcaccg gtaaaaatag
     1981 cagaaaaaat agagcattat ctaatttaaa aagggataac aatgaaaggt attattttag
     2041 ctggtggttc agggacaaga ctttatccaa tcacaatggg tgtatctaaa cagttactac
     2101 ccatttatga taaacctatg atttattatc ccttatctgt tttaatgctc gcaggtatta
     2161 gagaaatact gattattaca acacctgaag accaagatgg ttttcagcgt ttacttaatg
     2221 atggttcaca attcggtata tcacttcaat atgcaataca accaaagcct gaaggattag
     2281 ctcaagcatt tattataggt gaagaattta ttggtagtga tagtgtctgc ctagttttag
     2341 gagataatat attttgggga caaggattct caccaaaact gataaatgca gctaataaaa
     2401 aaaatggcgc tactgttttt ggttatgaag tacaagatcc tgaaagattt ggagttgttg
     2461 agtttgataa taatttaaat gcaatctcta ttgaagaaaa acctgaaaaa ccaaaatcta
     2521 attttgcagt aactggctta tatttttatg ataataccgt tataaatatt gcaaaaaaca
     2581 tcaaaccatc agaacgcggt gaattagaaa taacaactat taaccaagtc tatttacaaa
     2641 aaaatgaatt gagtgttgaa ctattaggtc gtggttttgc atggttagat acaggaactc
     2701 atgatagttt acttgatgca ggtacttttg ttgaaacaat agaaaaaaga caaggattta
     2761 aaatagcctg cttggaagag attgcatata accaaaaatg gttaactgaa gaacatatac
     2821 aaaaagcggc tattctgtac aagaaaaaca gctatggaca atatctattt aaattaatca
     2881 agaaagaaaa tgaaaataat taatactata tattttaata aattaggaga tgagagagga
     2941 tctctcacat cactagaaca aaataaaaat attccttttg aaataaaaag aatttattac
     3001 atatttgata caaagaaagg catttctcgt ggttttcatg ctcataaaaa gctacaacaa
     3061 ttagctattt gtgttcgtgg tagctgtcgc tttatcatgg atgatggaaa aaatagagaa
     3121 gaaattatat taaatgatcc aaatttaggg cttttaatta atcctatgca atggcacgag
     3181 atgcatgatt tttctgaaga ttgtatagta gtagttttag ctaatgatta ttatgatgaa
     3241 aatgattata tacgtaatta tgattcattt ataaactcat aggatctgat tatatgattt
     3301 taaaatctaa aacaatcaaa ttaagattcg ttaatgaaaa cgatgcagaa ttcattttat
     3361 ctttaagaac taataatacc tataatagat ttttatctca tgtagacaat gatattttac
     3421 agcaaaaaaa atggatagaa caatataaga aaaaagaaaa aaacaatgag gaattttatt
     3481 ttataataga aagattagat aatactcctt gtggtactgt aaggatttat gatattcaag
     3541 ataattcctt ttcatggggg agttggatat taaatgagaa taaaactcga actgccgcta
     3601 ttgaaagtgc attattagtc tatatatttg gttttgaaaa aaaatatttt actcactgtc
     3661 attttgaagt tatgaaagga aacgaaaaag taatttcttt tcatgaaaaa tttaatgcta
     3721 gaaaagtgga tgaagatgag catcattact tttatgaaat agaaccgaaa aatattatag
     3781 atataaaaaa taaatttatc aactttataa agtaggtgag acatggttcc gtttttagat
     3841 ttaaaaaaaa taaatgaaca atataccgaa gaattaaaaa atgcttgctc aagagttatt
     3901 gattctgggt ggtatataac aggaaaagaa ttggagaatt ttgaaaatga attctctaat
     3961 tactgtggta caatgtatgc aatcggtgtt gctaacggct tagatgcatt aactctagta
     4021 ttaagagctt ggaaagagca aggaaaatta caagcaggtg acgaagttat tatccaagcc
     4081 aatacttata ttgcatcaat tcttgctatt acagaaaatg acctagtacc tgtttttgtt
     4141 gaaccagatg ataaaacata taatttatca gttgataata ttaaatcagc aattacaaac
     4201 aaaactaaag ttatattgcc agtacatctc tacggacaaa ttaacccaat gaaagagatt
     4261 atggcaatag ctaaaaaaaa taatctttta gtcttagaag attgtgctca atctcatgga
     4321 gcaagtattg atgggataaa agcaggaaac tggggagatg ctgcaggctt cagtttttat
     4381 cctggtaaaa atcttggtgc actaggtgat gctggcgcaa ttacaactaa tgataaagaa
     4441 ttagctgata cagttatggc attacggaac tacggttcac ataaaaaata tgaaaattta
     4501 tatcaaggtg ttaatagtcg tttggatgaa attcaagcag ctatgctaag tgtgaagcta
     4561 aaatacttag atcaggaaac taaaattcgt caagaaatag ctctaaaata tctagaaaat
     4621 atcaagaatc ctttaattca attacccacc acagctgaag agtaccactt taataaacaa
     4681 agtcatgtat ggcacctgtt tgttatttca acaaagcata gggatttatt acaaaaatat
     4741 ttgatgcaac ataatatcca aacattaatt cactacccta caccacccca taagcaatta
     4801 gcatacaaac agtataattc gattaatctt cctattacgg aagagataca tgaaaatgtc
     4861 atttcattac cattttcccc tgtactaaat acaaccgaca tagattatat aatcaataca
     4921 ataaatgagt ttaaagtgtg aaacgacttt taaaggtttc agcaatgaca ggcctgttga
     4981 cactattacg tatgtcaatg ggtttcgtta ttgctaaagt tgttgctatt tacacaggtc
     5041 ctacaggtat ggctatgcta gggcaagttc agagcatggt tactagttta aatggtatta
     5101 ttaatgcccc tgttggtagt ggaattgtgc gctttacagc tgagcataaa gaaaaaggat
     5161 ttgatatttg ctcaatctgg tggagagcat cactttactg ggttgccctt attgcatcct
     5221 ttattattcc tattgggata tttttatcat catatattgc tgattggttg ctacatgacc
     5281 caacattagc atggattgtt attatcactg tatcagtact cccgttctca gcaataggaa
     5341 ctatttgtaa ttcaattata aatggccagc aactatatcg acgttttgtt ggtttaggta
     5401 tgctgtctac ccttatttca tcgagtgtaa tgctcacctt aatagtcact agcaatattg
     5461 aaggtgcttt attagcagca gctatacaat ctgcaattat tggtatcgtc atgtttattg
     5521 ctaatttaag acaaccatgg atgaagttcc gatattggtg gggaaaccaa gattataaag
     5581 ctcgtaaatc aatagctggg tatatgttaa tggctttaac cacggccacg acagtgccta
     5641 tttcattgat tttaattaga aatattctaa tttatcaaat aggctgggat gctgcaggat
     5701 attggcaagc tgtatggaaa atatcagagg tttaccttgg tgttataaca atggctttag
     5761 gtacatatta tttacctaaa ttatctgcat taaaaagtgc agatgagatt attattgaaa
     5821 ttcataaaac agccaaaata attttaccta tcgtaataat aatgtcagtt tgtgtatacc
     5881 tccttagaga tgttgcaata tggttattat ttactgaaga gtttaagccg atcagagact
     5941 tatttttcat acaactatgt ggagatattg tcaaaattgc cagttggctc tatgcctatc
     6001 caatgctagc aagagggact gcaaaatggt ttattttcac tgaaattata ttttcattct
     6061 cttttgttat ttttagttat tttttagtta ctaatttagg ccttgaaggc gttacatatg
     6121 catatttatt taattatata atctattttt tatttatgtt ctttaatata aaaaaaatta
     6181 ttcgataata aaagacaatt actcagatat attggtattt cctaatgaat tataatgagt
     6241 ttctagatat ttatttttca aatctttctt tatttttgac ttgtggtttt atatctatta
     6301 ttctttgttt ttattctgtt aatttcatta gtattgccgg gattacagat ccaatacact
     6361 ttttttggac ttatacatta ggaacaactt atagtataat tataggatta tttatacttg
     6421 gctatattag taacgatata attattttaa taggaatatc tattgtttct ttactattat
     6481 caatttatat aggatatgct tttaaaataa atctgttaaa gcatccaatt aattttatta
     6541 tagaatataa taatggctat cgtttattta aaattctttt atttttaatg tgtgcttttt
     6601 acactgtaat tatatataaa gtgggatttt ctattttttc tgaagtaaat aggtttgaac
     6661 aaaataaagg cattggacct cttgttagag tcgttgatgc aatcaggcct ttcgttttat
     6721 cctatatggc aatatatatt ggagtttata ccaaagggta taaaaagata atttacaatt
     6781 ctattttaat atctttaatt attgtcagct ccctgagtaa tggtgcaaaa tttgctttat
     6841 tagaaatagt atatgctatc tctgcttgtt ttattttata cagagggcat cgcttaaaag
     6901 tttcaatgaa acgcctaata aaaatatttt ctattttttt agttgttttt tcttttgctt
     6961 taattgcaac taattatgca ataaaagcaa aaggtgaaaa tatgaaacct caatttttac
     7021 cagaaaacac tcccatagct atagaaaaaa tatatttaag aactctctct aatggtgatc
     7081 aagcttactt aggattacct aatgaaatta taaataaaat tcctactgga aatattattt
     7141 ataattttac atcatctatg attggaaaag aaaaatttaa aaatatcttt ggtattaatg
     7201 ataatttaaa taatgttggg caaaaaatat tattatttca ttttcctgat tatgatcaag
     7261 caggaggccc tgtaagtcat tttgatttat atttttacca ttatttaccc ataggtttaa
     7321 attatatagc aatatttgtt ttgggattag tattagtatc cattgtaaat tgtaaagttt
     7381 tagcaaaagg aaatattttt atatcagcac ttatttccac cctttggatt agaggattag
     7441 ttatgatttt agagcctgct atgggatttg tgtatatatt agactttcta tttatcatat
     7501 ttttcataac attaatcact attattctac ctaaaaaaag gaaaaaggat ttcattaatg
     7561 ttaagtaaag aaaataaacc attagtgagt tttattctag tatcatataa tcaagaaaat
     7621 tatattgaag aagcaattaa ttctgtattc aatcaatcat atgaaaatct acaaataatt
     7681 attagtgacg atggaagtat tgatcacaca aagaatatta ttctagaaaa aataaaaaac
     7741 agagatgttt tatttttaaa taataaagaa aacaaggggt taattggtaa tttgaatctt
     7801 gctttttctt atgctgaagg tgatattatt atcgctatgg ctggtgatga tatttcaatg
     7861 ccaaatagag tcacaaaaat tgttgagtca ttcattaaaa atcctgatgt attttgtata
     7921 tattccaata cgatcgatat agataaagat agtgtagaaa tacctaattc ttcttacggt
     7981 ttaaaatacc atcataaaaa tggtgaaatg actattgaaa gacatcttaa agaaaattta
     8041 ggtatattag gatgttcagc tgcttttaaa agatgtttag ttacatcacc tttagcacat
     8101 tttttgccaa gcgaagataa agtattaact ctccgggctt taattaaggg taaaattctt
     8161 ttcattcctg aaaagttagt taaatataga ttgggcactg gaatatctaa caatctaaat
     8221 aaaaaaaata aatttgaata taaaaaaaat ctcaaagcta gaattctaac gattgatggt
     8281 tatattttag aacttaacaa caattcaatg tttttgaatg aaataaaaga tttaaagata
     8341 attaaagatt ttttaacttc ggcattaaat attgtagaag caaaaaaaat atccttacca
     8401 aaacattttt ttaatttaaa atctattgat tttaaagata aattgagatt tttctattat
     8461 agattttata gggattaatt caatatgtta tccaaattaa aaatgttaat atcatctatc
     8521 tattatactt tcttattttc cataagaaat atatttctta taagaacagg aaaaagtaat
     8581 agcattatta aaaaacaccc cattatagta accttaacta catatcccaa aagaattagt
     8641 actgtattcc ttaccattga gtctattttt tctcaaacta tttcacctga aaaaatagta
     8701 ttatggctat ataaagaaga taagccatta ataccaagct ctttaagaag gctacaaaaa
     8761 agaggactag aaatctattt ttgtgacgaa gatttacgtt cgtataaaaa attatcttat
     8821 acaaataatt tctttaatac ccatattata gttacagctg atgatgatat tttatatcct
     8881 aaaaattggt tagaagaact ttataatgaa tcattaaaat atcctaatac agttatctgc
     8941 catagaggcc atgatattag catttcaaat aatgaaatag aaagctattc aacttttatg
     9001 aagaataagg tatattcaac caatccttca ccattactta ttccaactgg atgctctggt
     9061 attctctacc ctatcggctc actttctatt gatgcatcat ataaaaaatc cagcattttt
     9121 atgcaattag ctcctgatgc tgatgatttt tggtataaat ttgtctgttt gaaaaatggg
     9181 tttcttgcta aacgaataaa ttctgaaaat aagcatttcc cgccaattat atcgtcatta
     9241 aaagatggat tgtttcataa taatgtaaga aataatcaaa atgataaaaa gcttttatca
     9301 actataaatt actttgaatt atatgatttt attataaaaa aataatacta attcaagatt
     9361 tattattaat ttatctcatg agtatcaaat ccaatgaaaa tcacaatctc cggaaccggc
     9421 tatgtcggtc tatctaacgg catacttctt tcccaaaaca acaacgttgt tgcgttagat
     9481 atcaataaag aaaaagttga attactgaac aaaaaaatct ctccaattac agataaagaa
     9541 attgaagaat ttttggcaac caaagagctc gattttaccg caaccttaga taaagaattc
     9601 gcttataaag atgctgagtt tgttatcatt gccacaccta ccgattacga tcctaaaaca
     9661 aactacttta acacctcatc agtagagtct gttatccgtg atgttttaga atataatcca
     9721 aacacgacaa tgattataaa atcaaccatt cctgttggtt ttacgaaaga aatgagaaag
     9781 aaatataata cagataatat tattttctcc ccagaattct tacgcgaagg tcgcgcactc
     9841 tacgataatc tttatccatc tcgtattgtt attggtgaac aatcagaaag agcacaaaaa
     9901 tttgctgacc ttttacagca aggtgcactg aaaaaagata ttgatgttct gttcgctgat
     9961 tccacagaag cagaagcgat taaattattt gctaatactt atttagcatt gcgtgttgct
    10021 tactttaatg agttagatag ctacgctcaa gcttatggct taaatgcaaa acaaatcatt
    10081 gaaggcgtct gtttagatcc gcgtattggt aaccattata ataatccatc gtttggttat
    10141 ggtggttact gcttaccaaa agataccaaa cagctgttag caaactacga ctctgttcct
    10201 aataatatta ttagtgccat cgtggaagca aaccgtacac gtaaagactt tattgctgac
    10261 tcaattatcg ctaaatcgcc aaaaatagtg ggtgtttatc gcttaattat gaaatctggt
    10321 tctgataatt tccgttcttc ttctatccaa ggcattatga aacgtattaa agccaaaggt
    10381 attgaagtgg taatctatga gccagaaatg aaagaagaaa ccttctttaa ctcaagatta
    10441 gttcgtgatt taaatgagtt taaatcaatc tctgatgtca ttattaccaa tagaatgtca
    10501 tctgtactaa acgatgttga agaaaaagtt tatactcgtg acctatttgg taacgattaa
    10561 ttatatattt ttctttaatt aggtcgtagt tctaatgtta tccaaaaata ctaatttttt
    10621 aatatcaatc attatgccat cctataattc aggagagtat atacggaagt ctatagattc
    10681 agttttgaga caatcctata aaaatcttga attaataatt attgatgatt gctctaatga
    10741 taataccatt gaagtaatat cctcttataa agatgagagg attaaaatat taaaaaatga
    10801 atctaatcaa ggtgctggaa ttactcgtaa taaaggatta tcacttgcta atgggcgttt
    10861 tatttgtttt ttagatagtg atgattattg gcgagatgat aagttagaaa aacagcttaa
    10921 ttttatgcta tctaataaca tagatttttg tttttctgct tatgatagag tttctgagga
    10981 tgacgctttt atctgtaaga ttggtgtccc tgaaaaagta aattactcag acatgctaaa
    11041 agtttgttct attggttgct taacagtaat gtttgataaa gacaaattta ctgatttttc
    11101 aatgccatca ataagaaaaa gacaggatta tgctctctgg ttaaagctat tgaaacaaac
    11161 accttacgcc tatggaataa gcgaatctct cggttcttat aatataagag aaaattcaat
    11221 ctcctccaat aaacttgacg cagcaaagca ccaatggcgg atttatcgag atatagagaa
    11281 tcttaatata ttttcagctt cctattattt ttctcactat gcttttaatg gtttttttag
    11341 atctaaactt ccttctttag ctaaaaagct gggtcttttg aaataatatt agtttctaca
    11401 tcttgtctta ggctttatat gctcaatatc gtcttatttg aacccgaaat tccaccgaat
    11461 acgggtaata ttatccgcct gtgtgctaac acaggctttc atcttcatct aatccaacca
    11521 ttgggtttta cttgggatga taaacgtcta cgccgtgcag ggcttgatta tcgtgaattt
    11581 gctgatatta aacagcatca cgattactat gcttttttag aaagtgaagg tttaaaccct
    11641 gataatacac aatcaccaac aggtgcacgt gtatttgcgt taacgacaaa aggcacacca
    11701 gcacatagta atgtgagcta tcaagatgga gattatctcc tttttggacc tgaaactcgt
    11761 ggtttaccac cttacgtgtt ggataatatg ccaacacagc agaaaatcag agttccaatg
    11821 cttgctgata gccgtagtat gaacctgtca aataccgttg ccgttgtggt ttttgaagca
    11881 tggcgtcaac ttggttatcc tggtgcgtta ttaagagatt gatctttcga tacaaaaatg
    11941 accttatgga atttatattt ctaataaggt cattttattt atatttcaag tacagcacat
    12001 ttccaatcta ttttttctta ttaaattttc tcatcaaaaa ataccacctc gctcttagtt
    12061 tcattaaaaa tgatgaaaaa tcatttacta ataccttcct ttctaagatt tttttcttaa
    12121 agctattttt taattttagt tcttcactat attcttcagc attaaattgt tgatcaatct
    12181 tttcaagtag agctttacac ctgactactt gttctttagt ttttttatta ttatctaata
    12241 ataatgaata actctcttca ttaatgcgaa taccaaaatg ctttaatagc tttcgcccta
    12301 atctttccgc tgtatttttt tgaatttctt tatatcgaga acgatttacc gttgggtgag
    12361 tacgataata aagtaatggt ataggaagat tatggagctt aaatctctct gtaatgatta
    12421 aatttaccca taacccataa tcttctgcat aaagatcttc tttatcatat ccaccaattg
    12481 ataatatgtc tttcttacga taaataacgg ttggatgagc taatgaacaa tcgtaaaggc
    12541 tagagataac aatatctgta tgtgtttcag ggtaaatatt aattctatcg ggatcttctt
    12601 gataaataaa accgccacca catacactga tattggggtt atctaccata aattgatact
    12661 gttcttctaa tcttgaatca atcgctatat catcagaatc cattcgagca ataagatcac
    12721 atgaggcaag ctttaatccc ttatttaaag atgcaattaa cccaatattc tcttcattga
    12781 taacaacttt tattcttgga tctttttcag cattttcttt taaaatatta aatgaattat
    12841 ctgttgagcc gtcattaatg ataagaaact caaattcagt gaatgtttgg tttaatatag
    12901 aatctataga ctcttgtagg tacttctcat cattatatac cgacattaat acggtgattt
    12961 taggcataat atttttcatc tatatatttt cagcaatatt tattaatctc aaaactcatc
    13021 ttattaatat acctacaccc ttcccccaca tcctttaata ccaatgcgtg agcaccaatc
    13081 actgcattat caccaatctc tatctctccg ccaataatcg aagaattaca accaatataa
    13141 acatggttgc caactcgaat atctgcttta ccatcttttt cttttacacc gatggtgacg
    13201 ccttgcatta acgtcacgtt ctcacctaat tggcagtaat tggtcagtac gatccctatg
    13261 ggatgtgcta atgaaagacc tttacctata cgagttctta gtgaaatatc gttgcagtac
    13321 ttgcgattat ttttagcgcc aatacgaatg ccaagttttt taagaaaacg acttttagag
    13381 aaataaaaga attgggcgac acgccaccaa aatagataac gtgaacgttg gcagaaaaag
    13441 acgcgatgaa atagtctcac ccaactaaaa ggcttgttat caccgatata ttcagcatgt
    13501 aaacaagcct tcagctcaga gagtgtaact tcagttaatg ccattaaatg ccatcaccac
    13561 attcaaaacc gggcaccaca ccattaaagt gttgatccat atcgagtgaa ggcttgtcac
    13621 tcgttggctt accaacaatg cgggcaggaa cccctgcaac agtggtatga gcaggaactt
    13681 catgtaatac cacagagcca gcaccaattt tggcaccacg gccaatctca atattgccga
    13741 gtattttagc acccgcgcca atcattacac cttcgcgtac tttagggtgg cgatcaccac
    13801 aggttttacc tgtaccccct aaagtgacgg attgtaaaat agaaacgtca ttctcaacaa
    13861 tggcggtttc accaatcaca atacctgttg cgtgatcaag cataatgcca tgaccaatgc
    13921 gagcggcggg atgaatatca accccaaaag tcacagaaat ctggttttgt aggtaaatgg
    13981 caagtgcttt acgatcttgc ccccatagcc aatgcgctat gcgataagct tgtaaggcat
    14041 gaaaaccttt tagataaagc agaggtgtag agtatttatc aacagcggga tcgcgtagac
    14101 gaacggctga aatatcgtat gccgcagatt caatcataga attatcacta cgataggctt
    14161 cttcaacaat ctcacgaaca gcaatcgcag gcatagtctg agttgccagc ttgttcgcca
    14221 gcatatagct taaggcactg cctaagtttt catgcttgag taatgtcgca tggaagaaac
    14281 tcgccaaaat cggctcacaa tctgccaaac atcttgcttc atctttaata tgatcccaga
    14341 ctctttttag ttcttctagc gacatactca ctctcattct ttttacattg aagcctaagt
    14401 aaaaacttag gcttcttatg tttaaaacag aaataataac tatctaaaat agacttagaa
    14461 aagattgctg tctatctcat ctttcgttgc acggcctaat aaagataatg cagcttcttt
    14521 ggcatcttta tgctggtata aaatttggta gatctgctct gtgataggca tttcaacacc
    14581 aacttgttct gctaatgcgc gaacttcttt agtattgcga taaccttcaa cgacttgccc
    14641 aattttttct tgagcggtat cgacatcaaa accttggcct agcatcatac caaatcgacg
    14701 attacgagat tggttatcag tacaggttaa aactaaatca cctaaacccg ccattcccat
    14761 aaacgttgcc gcatctgcac ctaatgcttt acctaaacgg ctcatttcgg caagtccacg
    14821 ggtgatcagc gctgtacgcg cattcgcacc aaaacccata ccatcagaca tacccgcacc
    14881 aatcgcaatc acgtttttta ccgcaccacc taattgcaca ccgatgaagt caggattttt
    14941 ataaacacgg aaacttttgc cacaatggaa aagctgttga agctcttcag agaacgtatt
    15001 atccgttgat gccacagaaa tcgcagtggg taaaccagcg gcaagctctt tagcaaaagt
    15061 agggcctgat aaaactgcta gcgggatttc attacctaat acttcacgcg ctacatcttg
    15121 caacaagcga ccagtatgag cctcaagtcc ttttgtcgcc caaacaacac gtgcatcctg
    15181 acgtaaaaag ggtttgatct gttgtaatac atcaccaaaa acatggcttg ggatcacaac
    15241 aagaatatta cggctggctt caatggcttt ttgcaaagaa gcttccatgt ataaactatc
    15301 aggaaaggaa acatcgggca gaaatgcctg attacagcgt gcttgttcta acgctgcaac
    15361 atgctcagga tcatgacccc aaagtacgac gtcatgacca ttgcgcgcta aggtaatcgc
    15421 taaagcggtg ccgtatgaac cggcaccgat aactgtcata gaagcgttca ttaagcttct
    15481 tcagcccctt cattagcagc atcagtttgt tgctgttgta aatagttcat aaacaacgca
    15541 tcaaagttaa caggtgctaa gttcagttgt gggaaagtac cacggctaac taagttagtg
    15601 atacat
//
