LOCUS       KY710697               17464 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 34/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710697
VERSION     KY710697.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 17464)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17464)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17464
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 34/57"
                     /serotype="O18"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     gene            1..813
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..17464
                     /note="O antigen gene cluster"
     CDS             896..2041
                     /note="UDP-galactopyranose mutase"
                     /codon_start=1
                     /transl_table=11
                     /gene="glf"
                     /product="glf"
                     /protein_id="AXY99502.1"
                     /translation="MKFLVVGCGLTGAVISRHLADAGHCITIIDQRSHIAGNCHTERD
                     RNTGIMEHVYGPHIFHTSDEVVWEYINKHGEMIPYVNRVKTTYQNQVYSLPINLHTIN
                     QFYQKALNPEQAKTFLSTITDKSITDPKNFEEQALKFIGKDLYEAFFKGYTVKQWGCD
                     PKNLPASILKRLPVRFTYDDNYYSHKYQGMPKHGYTDIVRSILDHKNIQVITNKKYDH
                     SESKEYDHIIWTGKIDEWFGYCEGRLNYRTLDFIKHETIGDYQGTAVMNYGDIAVPYT
                     RIHEHKHFSPWESHDKTIYIEEHSRVCEENDIPYYPIHLEEENKILNKYLELAQKEKY
                     ITFAGRLGTYRYMDMDVSIKEALMLAENLVSDLINQNELKSFYHKHQ"
     CDS             2056..2814
                     /codon_start=1
                     /transl_table=11
                     /gene="wemA"
                     /product="wemA"
                     /protein_id="AXY99503.1"
                     /translation="MSKFLAVIRCGDNSLHKNWVNDNAQFDVILSYFGEDIPYSLDKI
                     KYVHHFKGSKWEGLYDLFHNHPELWADYDYIWLPDDDLDSTVENINLFFELMQKYRFD
                     LCQPALTNNSYYSYKDLLQEPDLIYRETNFVEVMAPCFSKKIISKAYQTFNENKSGWG
                     LDFYWPILFKEENIKVGVIDSTPIHHTRPVGIAGHGSSDKTLSPLNELHQLLDKYSLE
                     IPTFVATSLLTRKGKMIQKGSFSYSLREFMKKRK"
     CDS             3287..4216
                     /note="orf04"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf04"
                     /product="choline kinase"
                     /protein_id="AXY99504.1"
                     /translation="MLTFINTLTKNFMINNSYTFITKIVNQTIAEPVLKIKKIGGMTN
                     TNYYCETLHTKAVVRLPGENTDVLINRKNEKLNCQKATKIGINPKLYYFNIENGIKIT
                     EYINNAITLTPETIHNKNILKNIALLLKKLHHSNIVFENKFNVFDEYRRYSNNLRNIK
                     IKYPNFKNVEKYFLKLEKRIDTLGVNLSPCHNDTVPENFIYNQYNYYLIDWEYSGMND
                     SIWDIAALFEESNLSKNDEDLFLTYYLDKEFVNYDKIKEKIIIYKICQNFLWSIWTII
                     KEQNNSLFGNYGIERYNKCQKQIELHETTYASI"
     CDS             4203..5141
                     /note="orf05"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf05"
                     /product="hypothetical protein"
                     /protein_id="AXY99505.1"
                     /translation="MLQFNKFTIIGLLSGFFWAIDTILIDYNISSINLFNFTILFSLS
                     IALLHDFFSSIWLLLYIIISKRTNVLIKSLKEKNNYIICLAAVSGGPIGMTCYMLGIY
                     YGNSSIAATISTIYPALGCLFAYIFLKIKTRIINFIGLIIVIISTIYIGLNLESFSQL
                     SLLGFLFSLGCATGWGLESTICSYALKNKLDYNIALLLRQSTSSIIYLIIILFFYKTE
                     IISIINIINSYSLISIIAAIAIFGTISYLFYYKSIDSLGPIKAMGLNISYSAWAILLG
                     SILGYGLSPLLLLISIIIITGSLITNLKTKEDAIIK"
     CDS             5138..5839
                     /note="orf06"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf06"
                     /product="Nucleotidyl transferase"
                     /protein_id="AXY99506.1"
                     /translation="MNAIILAAGLGSRFGDITKKTHKALLPIGNTANIERTIQYLIEF
                     GITDIYIVVGYLADNFQYLKKKYNCHLIYNNYYNKFNNLFSFYVAKEYFSDTYMIDAD
                     VVFLNNFLEKHQYSTYYLINRTENKSEWIPILDKDQFVKKIDIASYDAPSLLGVSFWK
                     KDDCILIKNKLKSLFLEKKEYENFANNYWDDIPKSIIHNMKIKTKLLNPTDAGEMDTL
                     DNYLQIKELYNNSKK"
     CDS             5847..7103
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99507.1"
                     /translation="MNIINKIIKHSIFKNTLALSIIKILDIILPLITIPYLTRVLSLN
                     DFGLLIISLAIYNLANILTDFGFGLSTPYTIAKNKNNSIFLNNYISSIFILKIILFIF
                     SIVFIVLYYNSINSHIIDITIIYLTIAIVLSLTFQTPWFFIGIEKMKTITLLASLSKL
                     LYFFLIFLIVPYYKSINSVLLSIFISNLIASIFYFILIRKFGIQFKLVKFKYIIIIFK
                     ENIGFFISRISVHLSTTMNGILIGSILGSTSSAIYGAADKLYNAGIGLMSPISNALYP
                     NMARTKNLKLLIIITLFLFFTSSIFCIIISNFTDIIIPTIFGSKYNDSVQYFNLMLYL
                     VPINIISILFGYTAFSIIDKTNITNYTVIISAIIYIISLLILYFTKQFSIKNLIYMII
                     LIDSITLFFRLTIFSFLYSRKKQSVK"
     CDS             7093..7485
                     /note="orf08"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf08"
                     /product="LicD-family phosphotransferase"
                     /protein_id="AXY99508.1"
                     /translation="MLNKFETLRYAQEILISSLDHINNICKNNNISYWIDSGTLLGAY
                     RDGSFIPWDEDIDLCMTRENYDKFLSVVYEQLDNEKYTLQNINTDKYYTLFPVPSKIR
                     VNNTRILWGLTRKVFNINTQKNHIREYI"
     CDS             7497..7919
                     /note="orf08_2"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf08_2"
                     /product="LicD-family phosphotransferase"
                     /protein_id="AXY99509.1"
                     /translation="MDNFLESKRTTRNFIIRLYKILYLKRHNNLSTIKKIISNISKYF
                     INRKFIDKINKYYYENINKKNTEYLDYSCDMALQEYKFPKNIIFPLKEIVFEGKKYPC
                     PNKTKEYLILLYGNNFMTPPNNPYQHGKITKLYDKEYE"
     CDS             8285..8956
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99510.1"
                     /translation="MHNIPDLSENFVYFNDDTFLINDISPSFYFNKMGNPKDACIFNL
                     IVDDGELSFYILNNLKIIEKHFNKWHELKTKPFNYFNIKYKSSQIKNFLLLFWKNFTG
                     FQNFHLPQAFNKKTFIELWIAEKEILINTCNNKFRNRYDVNQYLFRYWQLLKGNFEIS
                     NISSTGLCISPTPNNFTKIKKIFNSKKIKVVCINDNESISNFNTSKEFINSLLRKKTS
                     KQMHI"
     CDS             9418..10032
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99511.1"
                     /translation="MNNRSMSFVLWILISSTFHSSTLILLPFALLKKIRCNNFLFFII
                     TLIISVLIFQFDLASKILSIEFISQTKYANYINGKFSSETKLGSGFGVILKVIYPFII
                     LLFTMNRKDKSSNYIFTFMMMYIISIFLSAKIHIFNRIPPIFSVIIPFSVCYVYNIRT
                     KYKQLIIFSFISIYLFLYEVDINISQSSLNSGLGISPYTTIFEK"
     CDS             10078..11037
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99512.1"
                     /translation="MKIKYSVIIPAFNIEGYLHNIVNFTEEVLSVRHDIEIIVIDDGS
                     TDNTSLIKERHKNILYIYQKNGGVSSARNHGLKVSSGEFVLFLDADDSYDVSLFNELD
                     LFTINKEANIFLFNFLINKKPQNINLKLSNNNINWDLLSEYFFTRKIKLHICSICFRK
                     KHLLENNILFPVGYSFGEDIFFILLSIYKENSIFYIKKPLYNYNFHHGSVVNTVVNYN
                     KTKVLELYLKLLENEYKNNPFIVYFIQRTYLYLIKLCLIHKVSDERTVINLKKYKPIL
                     KIKTPYNNKPFIIIKNIMLLTSKFVFLLINKNSKVKSNLKATP"
     CDS             11053..11874
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99513.1"
                     /translation="MNNDNIAVIMSIYKNDNILYIKKSVQSILNQTQVNLLLFIHIDG
                     EIDESILVYLNKLASESKIILSQSKINQGLSISLNLLIEKVLFNPKIKFIARMDSDDI
                     SLPERLSIQMGYLENHPDIDILGTACEEFGSSFSLQKKSLPSHHDDLVKYSICRCPFI
                     HPTVMFRRKIFDDGNRYPTHTTFTEDMAFWLELIYKGYKLHNLPDVLLQYRLEDNTMH
                     RRKGLSKAKSEFSLRMGYMFLLNQVSLKNILLISSRYIFHLLPPNIMSKAYKYLR"
     CDS             11936..13102
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99514.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNQKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLDAFKAMSDVIITNRMATALNDVEEKVYTRDLFGND"
     CDS             13114..13617
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXY99515.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(13755..14732)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXY99516.1"
                     /translation="MKNNMPKITVLMSVYNDEKYLQESIDSILNQTFKEFEFLIINDG
                     STDNSLSILKENAKKDPRIKLIINEQNIGLIASLNKGLQLASCDLVARMDSDDIAVST
                     RLEAQYQFMLQHPEVSVCGGAFIYQEEPNRINTYPETHSDIVISSLYDCSLAHPTIIY
                     RKQDILSVGGYDKADLYAEDYGLWVNLIIAGKFKLHNLPVPLLYYRTHPNVERSQYKA
                     IQKDTAERVGRKLLMYFGINVNKEDYSLLLDNDKKTVEQVVRCKRLLEKINQQFNAKE
                     YRKELIQKNEFKKRLLERKVLVTDFSSFLIKVSARWYFLIRKITKKAKK"
     CDS             complement(14742..15299)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemH"
                     /product="wemH"
                     /protein_id="AXY99517.1"
                     /translation="MHAQAKVTLAELKECLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGNHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGEGYRYINKMNFEINKYC"
     CDS             complement(15302..16135)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99518.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(16235..17248)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99519.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNLDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
     gene            complement(17248..17463)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     6205 a   2813 c   2552 g   5894 t
ORIGIN
        1 cctcaaaatg cggcggatga tgtggcaaaa ggtaatctgc gccctcaccc tgaactggaa
       61 accggcccgc aagaattttt agcggcaggc accagtttta atcagatgat cagcgcctta
      121 gaacgcatgg ttgaagcgca acagcgattg atttctgata tctcccatga gttgcgcacc
      181 ccattaactc gtttacagct tgccagtgca ttactacgtc gtcgtagcgg tgaaagtaaa
      241 gagctagagc gtattgaaac agaaacacag cggctagatg gcatgatcaa tgacttatta
      301 gtgctttccc gcaatcaata taaaaatgag ttattacgcg aaaccgtaaa agcgaatgag
      361 ctttggaacg atattttaga taatgcgaaa tttgaggctg aacagagcaa taaaactcta
      421 acagtaaccg ccccgccagg cccatggacg atttattgca atccttattc attagcgagt
      481 gcctttgaaa atatagttcg caatgccttg cgttattcac attctcgtat tgaagtggct
      541 tttaccgaac aaaatcaggg tatcactatt attgttgatg atgatggccc aggggttagc
      601 ccagaagatc gcgagcatat tttccgacct ttttatcgta cggacgaagc acgtgataga
      661 gaatccggcg gtacagggct gggactggca atagttgaaa ccgcaattag tcaacatcga
      721 ggccatgtaa aagccgatga tagcccatta gggggattaa gagtggagat ttggttgccg
      781 agagggagta ttagctcaag caataaatta taattatcat ttttaactat tgataattac
      841 tgtatatttt accctattaa tttatacaaa atgtactaag ttcttagggt aaaatatgaa
      901 atttttagtc gtaggctgtg ggttaacagg ggctgtaatt agtcgccatc tagctgatgc
      961 tggtcattgc atcacgataa tagaccaaag atcacatatt gctggtaatt gccatacaga
     1021 acgagataga aataccggta ttatggaaca tgtttatggt ccacatatct tccacactag
     1081 cgatgaagtg gtatgggaat atatcaataa acatggtgaa atgatccctt atgtaaatag
     1141 agtaaaaaca acttatcaaa atcaagtata ttctttacct attaacctac atacaataaa
     1201 tcaattctac caaaaagcgc taaatccaga acaagcaaaa acttttttaa gtactatcac
     1261 agataaatct attactgatc ctaaaaactt tgaggaacaa gccctaaaat ttattggaaa
     1321 agatttatat gaagcctttt ttaaaggcta tacagtaaaa caatggggtt gcgaccctaa
     1381 aaatctacct gcgagtattt taaaacgcct ccctgtaaga tttacttatg atgacaatta
     1441 ttattctcat aaataccaag gaatgcctaa acatggctat accgatatag ttcgttctat
     1501 ccttgatcat aaaaatatac aagtaataac aaataaaaag tatgaccata gcgaaagcaa
     1561 agaatatgac catattatat ggactggaaa aatagatgaa tggtttggct attgcgaagg
     1621 tagattaaac tatcgaacgc tagattttat aaaacatgaa actataggtg actatcaagg
     1681 tacagctgtt atgaattacg gcgatatcgc ggtaccttat actcgcatac atgaacataa
     1741 acacttctca ccatgggaat ctcatgacaa aacgatttat attgaagaac atagtcgggt
     1801 atgcgaagag aatgatattc cttactatcc cattcattta gaagaagaaa ataaaatatt
     1861 aaacaaatac ttagaattgg ctcaaaaaga aaaatatatc acttttgcag gtaggctagg
     1921 tacatataga tatatggata tggatgtatc aattaaagaa gcacttatgc ttgcagaaaa
     1981 cttagtatca gatttaataa atcaaaatga acttaagtcg ttctaccaca aacaccaata
     2041 acaagagaat attctatgtc aaaattttta gcagtaatta gatgtggaga taattcactt
     2101 cataaaaact gggttaatga taatgcccaa tttgatgtaa ttcttagtta ttttggtgaa
     2161 gatatccctt attctttaga taagataaaa tatgtacacc actttaaagg atcaaaatgg
     2221 gaaggattat acgatttatt ccataaccat ccagagttat gggctgatta tgattatatc
     2281 tggttgcctg atgatgactt agacagtacc gtagaaaata tcaacttatt ttttgaatta
     2341 atgcaaaagt atcgattcga tctttgccaa ccagcattaa cgaataatag ttactattcc
     2401 tataaagact tactacaaga accggattta atatatagag aaactaattt tgtagaagta
     2461 atggcgccct gcttttcaaa gaaaatcatt tctaaagcat atcaaacatt taatgaaaat
     2521 aaatcagggt ggggactaga tttttattgg cctattttat ttaaagaaga aaatattaaa
     2581 gttggtgtta tagactctac accaatacat catacacgac cagtagggat agcaggccat
     2641 ggaagtagtg ataaaacctt atcaccttta aatgaacttc atcaattatt agacaagtat
     2701 tcacttgaaa tacctacgtt tgtagcaact tcacttctaa cccgaaaagg gaaaatgata
     2761 caaaaaggca gtttcagcta ttctttaagg gagtttatga aaaaaagaaa ataaccctat
     2821 gattaaatta agaaattcac cataaaatca caataaaact acttttatat caaccataag
     2881 gactgttttt atattgaata aatgcagtct aattacctga tacaaagtag atacattttg
     2941 agtaatgtag ttttaacgct tacagcaata caataaaaaa attactacat taaaatattc
     3001 tgccatttat tgaagagtag gtggcatatt cttttcattc ccatctttat agatatttca
     3061 gaatgatctt gctaaatcag tcgatattcc gatacttata tatacctaat caatgttaga
     3121 atacccatca attacttgac ccgctggttt gaagagtcta atctcaaagc ggtttagcat
     3181 actgttaaag tatgcttata taataaaact cttatattac agatagataa gggttttaaa
     3241 agagaaattt tggcgctctg ggggccgtaa acccagtgcg gtagctatgc taactttcat
     3301 taatacacta actaaaaact ttatgataaa taattcttac acctttatta ctaaaatagt
     3361 aaaccaaacc attgctgaac cagtattaaa aataaagaaa attggcggaa tgacaaatac
     3421 taattattat tgtgaaacac ttcatactaa agcagtagta cgattaccag gagaaaatac
     3481 agatgtattg attaatagga aaaatgaaaa gctaaactgt cagaaagcta caaaaatagg
     3541 aataaaccct aaattatatt attttaatat agaaaatgga ataaaaataa cagagtatat
     3601 taataacgcc ataacattaa caccggaaac aattcataat aaaaatattt taaagaatat
     3661 agccttgtta ttaaaaaaat tacatcactc aaacattgta tttgaaaata aattcaatgt
     3721 gtttgatgaa tatagacgat attcaaataa cctaagaaat attaaaataa aatatcctaa
     3781 ttttaaaaat gtagagaaat actttttgaa attagaaaaa aggatcgata cattaggagt
     3841 caatttatct ccctgtcaca atgatactgt gcctgaaaat tttatatata atcaatataa
     3901 ttattatctt attgattggg agtattcagg tatgaatgat agtatttggg atatagctgc
     3961 cttatttgaa gagtctaatc tatcaaaaaa tgatgaggat ctttttctaa catattattt
     4021 agataaggaa ttcgttaatt acgataaaat taaagaaaag ataattatat ataaaatatg
     4081 tcaaaacttt ttatggtcaa tatggacgat tataaaagaa cagaataata gcttatttgg
     4141 taattatgga atagaaagat ataataaatg tcaaaagcaa atagaactcc atgagactac
     4201 ttatgcttca atttaataaa tttactatta ttggattatt atcaggtttt ttttgggcta
     4261 tagataccat actaatagat tataatatat catcaattaa tctatttaat tttacaatac
     4321 ttttctctct ttccattgca cttctacatg attttttttc ttctatttgg ttattactct
     4381 atataataat atcaaagaga actaatgttt taataaaatc acttaaagaa aaaaataatt
     4441 atataatttg tctagccgct gtaagtggag gccccatagg aatgacctgt tatatgttgg
     4501 ggatttacta tggtaactca tctattgctg ctactatttc aactatttat ccagcactgg
     4561 gttgcctatt cgcctacatt tttttaaaaa taaaaacaag aataattaat tttattggat
     4621 taataatagt aattatttct accatatata taggattaaa tctagaaagc tttagtcaat
     4681 tatcattatt aggattctta ttttctcttg gctgtgcaac tggctgggga ttagaaagta
     4741 ctatatgctc atatgcttta aaaaataaac tagattacaa catagccctt ttattaagac
     4801 aatcaacctc atcaataata tatttaataa ttattttgtt tttttataaa acagaaataa
     4861 tcagcataat aaatatcatt aatagctatt ctctaatatc gattattgca gcaattgcga
     4921 tatttggaac tatatcttat ctattttatt ataaatcaat tgatagttta gggccaatta
     4981 aggcgatggg acttaatatt agctattcag catgggcaat acttttagga tcgattctag
     5041 gatatgggtt atctccatta ttattattaa tatcaataat aataataact ggttcactta
     5101 ttactaattt aaaaacaaaa gaagacgcta taataaaatg aatgcaataa ttttagctgc
     5161 tggtttaggt tctagattcg gcgatattac aaaaaaaaca cataaagctt tacttccaat
     5221 aggaaataca gccaacatag aaagaactat tcaatatctt attgaatttg gtataactga
     5281 tatatacatt gtagttggtt atttagcaga taacttccag tatttgaaaa aaaagtacaa
     5341 ctgtcatcta atatataata actattataa taaatttaat aatttatttt ctttttatgt
     5401 agccaaagaa tatttttcag atacatatat gattgatgct gatgttgttt ttttaaataa
     5461 ttttctcgaa aaacatcaat atagtacata ctacctaatt aatagaacag aaaataaatc
     5521 cgaatggatt cccatattag ataaagatca atttgtcaag aaaattgata ttgcttctta
     5581 tgatgcacca tctctgctcg gcgtttcttt ttggaagaaa gatgattgta ttttaattaa
     5641 aaataaatta aagagtttat ttttggaaaa aaaagaatat gaaaattttg ctaataatta
     5701 ctgggatgat atccctaaat caataattca taatatgaaa atcaaaacta aattattgaa
     5761 tcctactgat gctggtgaaa tggatacttt agataactat ttacaaatta aagaattata
     5821 taataattca aaaaaataaa agcattatga atattattaa taaaattata aaacatagca
     5881 tattcaaaaa cacattagca ttaagtatca taaagatatt agatattata ttaccattaa
     5941 ttacaatacc gtatttaact agagtattaa gtctaaatga ttttgggtta ttaattatat
     6001 cactagcaat atataattta gcaaatattt taactgactt tgggtttggg ttatctactc
     6061 catataccat agcaaaaaat aaaaataatt ctatcttttt aaataattat atatcatcta
     6121 tattcatact aaaaataata ttgtttatat ttagcattgt ctttatagta ctttattata
     6181 atagtatcaa tagtcatata attgatataa ctattattta cttaacaata gctattgttt
     6241 tatcattaac ctttcagact ccatggtttt ttattggaat agaaaaaatg aaaacaataa
     6301 cattattagc ttcattaagt aagctacttt atttttttct tatttttcta attgttccat
     6361 attataaaag cataaactct gttcttttat ctatatttat aagtaattta atagcatcaa
     6421 ttttttattt tattcttata agaaaattcg gaattcaatt taaacttgtt aagtttaaat
     6481 atattataat tatttttaaa gaaaatattg gcttctttat ttctcgtatt tctgtccacc
     6541 tatcaacaac tatgaatggc attttaattg ggtctatact tggcagcaca tcctccgcta
     6601 tatacggagc agcagataaa ctttataatg ccggtatagg attaatgtca cctatatcta
     6661 atgcattata ccccaatatg gctagaacaa agaacttaaa attattaata ataatcacac
     6721 tttttttatt cttcacatct tctatttttt gtataataat atctaacttt actgatataa
     6781 taatcccaac tatttttggt tcaaaatata atgattcagt tcaatacttt aatttaatgt
     6841 tatatcttgt tcctattaat atcatttcta tactatttgg atatactgca ttttctataa
     6901 tagataaaac aaatataact aattacactg ttataataag tgcaattata tatataattt
     6961 cactattaat cttatatttt actaaacaat tttcaataaa aaatttaatc tatatgatta
     7021 tattaatcga tagtatcacc ctatttttta gattaaccat atttagtttt ttatattcaa
     7081 ggaaaaaaca aagtgttaaa taaattcgaa acattaagat atgctcaaga aatattaata
     7141 tcttcgctag atcatattaa caatatatgt aaaaataata atatatcata ttggatagac
     7201 tcaggaacat tgctaggtgc ttacagagat ggtagtttta ttccctggga tgaagatatt
     7261 gatttatgta tgacacgaga aaactatgat aagtttttat ctgtagtata tgaacaacta
     7321 gataatgaaa aatatacact acaaaatata aatacagata aatattatac attattccct
     7381 gttccatcta aaattcgagt aaacaacaca agaatacttt ggggactgac cagaaaggta
     7441 ttcaatataa atactcagaa aaatcacatt cgggaatata tatagatata ttccctatgg
     7501 ataacttttt agaatccaaa aggactacaa ggaattttat aatccgatta tataaaatat
     7561 tatatttaaa acgtcataat aatctatcta ctataaagaa aattatttca aatatatcta
     7621 aatactttat taatcgaaaa tttatcgata aaataaataa gtattattat gaaaatataa
     7681 ataaaaagaa tactgagtat ttagattata gctgtgatat ggctttacaa gaatataaat
     7741 ttcctaaaaa tataatattt cctttaaaag aaatagtttt cgaaggaaaa aaatatccat
     7801 gtcctaataa aacaaaagaa tatttgatat tactttatgg aaataatttt atgacccccc
     7861 ctaataatcc ttatcaacat ggaaaaatta ccaagcttta tgataaagaa tatgaataat
     7921 taatttttat taatacagtt tatgtataaa tggattaaat actaatgaat aaaaaaattg
     7981 attttgtcat tccatggtta gaccctacag atcctgaatg gattaagtct tacaatgagt
     8041 attctattga tgataaaatt cttgaagata atcctagatt tagagactgg gacaatttac
     8101 attactggtt tagaggaatt gatagatttg ctccatgggt caataaaatt cattttataa
     8161 cttacggtca cttgccatct tggttaaatg tgaatcatcc taaattaaat attgtcaatc
     8221 ataactgaat tatatagatc cttcgtattt gccaactttt agctcacatg tgctagaatt
     8281 aaatatgcat aacataccag atttatcaga gaattttgta tattttaatg atgatacatt
     8341 tttaatcaat gatatttcac cttcatttta ttttaataag atgggaaacc ccaaagatgc
     8401 ttgtattttt aatctaattg ttgacgatgg agaattgtca ttctacatac ttaataattt
     8461 aaaaattatt gaaaaacatt ttaataaatg gcatgaatta aaaacaaaac catttaatta
     8521 ttttaatatt aaatataaat catcccaaat aaaaaacttc ttattattat tctggaaaaa
     8581 tttcactgga tttcaaaact ttcatttacc acaagctttc aataaaaaga catttattga
     8641 actatggata gccgaaaaag aaatcttaat taatacttgt aataataagt ttagaaacag
     8701 atatgatgtt aaccaatatc tatttagata ttggcaacta ttaaaaggta attttgaaat
     8761 ttcaaatatt tcatctacag gtttatgcat atcaccaacc ccgaacaact tcacaaaaat
     8821 aaaaaaaata tttaactcta aaaaaataaa agttgtatgt attaacgaca atgaatcaat
     8881 atcaaacttt aatacttcta aagaatttat taattcattg cttagaaaaa aaacttccaa
     8941 acaaatgcac atttgaaatt taattatgat tttttactta tatagtatat aactcaattc
     9001 ttttatttag ttttttattt ttcttatatt gctgaatatt gtaaaaacaa gaaactctca
     9061 atcatattct tatcaggctc tttttttata atctttttta ttgctgcaat aaggtataac
     9121 ataggtaccg actataaaaa ttatgttgaa atatttaaaa acctaaacac atcagatcaa
     9181 tatttagagc tttcatttct attcataagc tcaactatta aatcaaatgg cttagatgta
     9241 caatggctat ttgtagtttt ttcttttata tcacttttat ttatatatat tacagccttt
     9301 aactataata aggttctttt cattatttct tttatcttaa tactttattt acaaaattta
     9361 tccgcgataa gacagatttg tgcgatatgt atttcttttt atgctattca taaattaatg
     9421 aataatcgct ctatgagttt cgtattgtgg atcttgatat cttccacatt ccattcatca
     9481 acacttattt tactgccatt tgcattactt aaaaaaatac gttgtaataa ctttttattt
     9541 tttataatta cacttataat ttctgtatta atatttcaat ttgacctagc aagcaaaata
     9601 ttatctatcg aatttatatc acaaacaaag tatgcaaatt atattaatgg aaaatttagt
     9661 agtgaaacca agcttggttc tgggtttggc gtaatactaa aagttattta tccatttatt
     9721 atattattat ttacaatgaa cagaaaagat aaatcatcaa attatatttt tacatttatg
     9781 atgatgtata tcatcagcat ttttctttcc gcaaaaattc atattttcaa taggattcca
     9841 ccgatatttt cggtaattat accattttct gtttgttacg tttataatat aagaacaaaa
     9901 tataaacaac tcataatttt ttcttttata tctatctatt tatttcttta tgaggtggat
     9961 ataaatatat ctcaaagctc tttaaatagt ggtttaggaa tatctcctta tacaactatt
    10021 tttgaaaaat aaatttctat ttcctatatc attaccatca cactttaata ttattatatg
    10081 aaaatcaaat attctgttat tattccagca ttcaacatag aaggatattt acataatata
    10141 gtaaatttca ctgaggaagt tttatctgta agacatgata ttgaaattat tgtaattgat
    10201 gatggctcta ctgataatac atctttaatt aaagaaagac ataaaaatat tctttatata
    10261 tatcaaaaaa atggaggtgt atccagtgca agaaaccatg gtttaaaggt atctagtggt
    10321 gaatttgttt tatttttaga tgctgatgat agttatgatg tttcattatt taatgaacta
    10381 gatttattca ctataaataa agaagcaaat atttttctat ttaatttctt aataaataaa
    10441 aaaccgcaaa atataaactt aaaattatcc aacaataata taaattggga tttattatct
    10501 gagtattttt tcacaagaaa aataaaacta catatatgtt caatatgttt tagaaaaaaa
    10561 catttattag aaaataacat tttatttcct gttggatatt cattcggtga agatatattt
    10621 tttattttat taagtatata taaagaaaac agtatatttt atataaaaaa acctttgtat
    10681 aactacaatt tccatcatgg cagcgttgta aacaccgtag ttaattataa taaaacgaaa
    10741 gttctagagc tctatttgaa actattagag aatgaatata aaaataaccc ttttatagta
    10801 tattttattc aacggactta tttatatcta ataaaactat gcctaataca taaagtatct
    10861 gatgaacgta cagttataaa cttaaagaag tataaaccca tcttaaaaat aaagacacca
    10921 tataacaata aaccattcat tataataaaa aacataatgt tacttacgag taaatttgtt
    10981 tttttattaa ttaataaaaa ttccaaagta aaaagcaact tgaaagctac cccataaaaa
    11041 ctaagatata aaatgaataa tgacaacata gctgtaatca tgagtatata taaaaatgat
    11101 aatattttat atataaagaa atcggttcag tcaattttaa atcagactca agttaatcta
    11161 ttattgttta ttcatattga tggtgaaatt gatgaaagta ttctggtgta tttgaataaa
    11221 ttagcatctg aaagtaaaat tattttatct caatctaaaa taaatcaggg gttatccata
    11281 tcattaaact tattaatcga aaaagtttta tttaatccta aaataaaatt tatcgctaga
    11341 atggatagtg atgacatatc cctccctgaa agactttcaa tacaaatggg atatctagaa
    11401 aatcaccctg atatagatat acttggtact gcttgtgagg agtttggttc ttcattttca
    11461 ttacaaaaaa aatccttacc ttcacatcat gatgatttgg tcaaatacag tatttgtcgt
    11521 tgccctttta tccatccaac tgtaatgttt agacgaaaaa tctttgatga tggtaatcgt
    11581 taccctacac atactacatt tactgaagat atggcttttt ggttagaatt aatatataaa
    11641 ggctataaac tacataattt accagatgtg ttgttacaat atcgattaga agacaacaca
    11701 atgcatagaa gaaaaggatt atcaaaagcg aaaagtgaat tttctttgcg tatgggatat
    11761 atgttccttc taaatcaagt atcattaaaa aatatattac taattagctc tagatatatt
    11821 ttccatttat taccacctaa tataatgagt aaagcatata aatatttaag atagagatat
    11881 ttccatcttc tattctatac aatcctattt aataaatttt agataagaaa aacacatgaa
    11941 aatcaccatc tcaggaaccg gctatgtcgg tctatctaat gggatactcc tttcccagca
    12001 caacaaagtt gttgcattag atatcaataa agaaaaagtc gaactattaa accaaaaaat
    12061 ctctcctatc tctgataaag aaattgaaga gtttttagcc actaaagagc tcgactttac
    12121 agcaacttta gataaagaat tcgcttataa agatgctgaa tttgttatca ttgccactcc
    12181 taccgattat gatcctaaaa ctaactactt taacacctct tctgtagagt cggttattcg
    12241 tgatgtttta gaatataatc caaacactac tatgattata aaatcaacca ttccggttgg
    12301 ttttactaaa cagatgagag aaaaatatca taccgataat ataatcttct ctcctgagtt
    12361 cttacgtgaa ggtcgcgccc tttacgataa cctttatcca tctcgtattg tgattggtga
    12421 gcaatctgca agagcagaga aatttgctga gcttttacaa caaggggcat taaaaaaaga
    12481 tattgatgtt ctttttactg attctacaga agcagaagcc attaagttat ttgctaatac
    12541 ttatctcgca ctgcgtgtgg cttattttaa tgaactcgat agctacgccc aagcttacgg
    12601 cttaaacgca agacaaatta ttgaaggtgt ctgtttagat ccgcgtattg gtaatcacta
    12661 taacaatcca tcatttggtt atggtggcta ctgcttacca aaagatacga aacaactgtt
    12721 agcaaattac gaatcagtgc ctaataatat tattggcgcc attgttgaag cgaaccgcac
    12781 ccgtaaagac tttattgctg attctattat cgctaaatca ccgaagattg ttggcgttta
    12841 ccgtttaatt atgaaatcag gctctgataa tttccgctca tcttcaatcc aaggtattat
    12901 gaagcgtatt aaagcgaaag ggattgaagt ggtgatttat gagccagaaa tgaaagaaga
    12961 gactttcttt aactcaaaac tcattcgtga tttagatgcg tttaaagcca tgtctgacgt
    13021 tattattacc aatagaatgg ctacggcgct aaacgatgtt gaagaaaaag tttatactcg
    13081 tgatctcttt ggtaacgatt aaaactaatt tatatgctca atatcgtctt atttgaaccc
    13141 gaaattccac cgaatacggg taatattatc cgcctgtgtg ccaacacagg ctttcatctt
    13201 catttgatcc aaccattggg ttttacttgg gatgataaac ggctacgtcg tgcagggctt
    13261 gattatcgtg agtttgctga tatcaaacag catcacgatt attatgcttt tttagaaagt
    13321 gaaggattaa gccctgataa tgcacaatcc ccgacaggtg cgcggttgtt tgcattaacc
    13381 actaaaggta ctcccgccca tagtaatgta agttatcaag atggggatta tctgctcttt
    13441 ggccctgaga cacgaggttt accaccctat gtgttggata atatgccaac acaacaaaaa
    13501 atccgtgtac caatgttagc agatagccgc agtatgaatc tttctaatac ggttgccgtg
    13561 gttgtttttg aggcatggcg ccaattaggc tatccggggg cattattaag agattaattt
    13621 atctttttaa tttataacca taaaaaatga cctctagact tcattgtctc gaggtcattt
    13681 ttatgtatat gctttgtgct agttttagct tttcttattt tatatcttct ttttatcttt
    13741 tttatctttc ggttttattt cttagctttt ttagttattt ttcttatcaa gaaataccac
    13801 cttgcactta cttttattaa aaatgatgaa aaatcagtta ctaatacctt tctttctaat
    13861 aatcttttct taaattcatt tttttgtatt aactcttttc gatactcttt agcattaaat
    13921 tgttggttaa tcttttcaag taacctttta cagcgtacta cttgctctac tgttttttta
    13981 tcattatcca ataataatga ataatcctct ttattcacgt taatgccaaa atacattaat
    14041 aattttcgtc ctactctttc cgcggtatct ttttgtatcg ctttatattg cgaacgttcg
    14101 acatttggat gagtacgata ataaagaagt ggtacaggaa gattatggag cttaaatttt
    14161 ccagcaataa ttaaattcac ccataaacca taatcttctg catagaggtc tgctttatca
    14221 tatccaccaa cagataatat atcttgctta cgatagataa tggttggatg cgctaaggag
    14281 caatcataaa gactagaaat cacaatatca ctatgtgttt ctggataagt attaattcta
    14341 tttggctctt cttggtagat aaaagcacca ccacaaacac tcacttcagg atgttgtaac
    14401 ataaattgat attgcgcttc tagtctggtt gaaacggcaa tatcatcaga gtccattcta
    14461 gcgactaaat cacatgaagc taattgtaat cctttgttta aagaagcgat caatccgata
    14521 ttttgttcat taataatcag ttttattctg ggatcttttt tggcattttc ttttaaaata
    14581 gataatgagt tatccgttga gccatcatta atgataagaa actcaaattc tttaaatgtt
    14641 tgatttaata tagaatcaat agactcctga aggtatttct catcattgta taccgacatt
    14701 aatacggtaa ttttgggcat attgtttttc attatctatt ttcagcaata tttattaatt
    14761 tcaaaattca tcttattgat ataccgatac ccttccccca catcctttaa tactaatgca
    14821 tgggcgccaa taatcgcatt atcaccaata ttaatctcac caccaataat ggatgaatta
    14881 cagccaatat aaacatggtt tcctacttgg atattggctt ttcccccttt ctcttttacg
    14941 ccaatagtca ccccttgcat aatagtgaca tattccccca gttgacagta attagtcagt
    15001 acaatgccaa tcgggtgtgg taaagaaagg cctttaccaa tacgagtttt taacgagata
    15061 tcattacaat atttacggtt attgctagca ccgatccgaa tacctaattt atgtaaaaag
    15121 cgatggggag agaaataaaa atactgtgct acccgccacc aaaataggta acgggaacgc
    15181 tgacagaaaa atactcgatg aaatagtctt aaccaactaa agggtttttt atcaccaata
    15241 tattcagcct gtaagcactc ttttagttca gcaagggtaa ccttagcttg agcatgcata
    15301 gttaaatacc atcaccacac tcaaagccgg gtacaacacc attaaagtgt tgatccatat
    15361 cgaatgacgg tttttcacta tcaggacgac cgacaatgcg ggcaggcacc cccgccaccg
    15421 tagtatgagc tggtacttca tgtaatacta ccgagccagc accaattttg gcaccacgac
    15481 caatctcgat attaccaagg attttagctc ctgcgccaat cattacccct tcacgcactt
    15541 tcgggtgacg atcgccccct gttttacctg ttccccctaa ggtcacagat tgtaaaatgg
    15601 agacatcatt ttcaaccacc gccgtttcac caatcacaat acctgtcgca tggtcgagca
    15661 taataccgtg tccaatacgt gccgctgggt ggatatccac cccaaaggtg actgatattt
    15721 ggttttgtag ataaatagct agcgcttggc gattttgttg ccatagccaa tgggcaatac
    15781 ggtaggcttg tagtgcatga aaccctttta aatagagtag tggggtagaa tatttatcca
    15841 ccgcgggatc acgtaggcgt accgcggaga gatcacaagc agccgactca atcattgagc
    15901 tatcgtgact ataagcctct tcaacgattt cgcgtactgc aatggctggc atagtctgag
    15961 ttgccaactt attggccagc atatagctta atgcactacc taaattttcg tgtttgagaa
    16021 gtgtcgcatg aaagaaactg gctaaaattg gctcacaatc tgccaatgca cgcgcttcgc
    16081 ttttaatatg atcccagact cttgttagtt cttctagcga catactcact ctcatttttt
    16141 acacgaaagc ctaagtaaat acttaggctt tgaattttca aacaaatgga tagttaaaac
    16201 agatacccta agaatattat ttaaaataaa ctgactacag gctgctgtct atctcatctt
    16261 tggttgctcg ccctaataag gccaatgcag cctcttttac atctttatgt tgatataaaa
    16321 tttggtaaat ctgttcggtg attggcattt ctacacccac ttgttcggct aatgcacgaa
    16381 cttctttggt gttacgataa ccttcgacta cctggccaat tttctcttgg gctgtatcaa
    16441 catctaaacc ttgacctagc atcataccaa agcgacgatt acgtgattga ttgtctgtac
    16501 aggttaacac caaatcaccc aaaccagcca tgcccataaa agttgccgca tctgcaccta
    16561 aggctttacc taagcggctc atttctgcca gaccacgggt aatcagtgcg gtacgcgcat
    16621 tagcgccaaa tcccatacca tcagacatac ccgcaccaat agcaatcacg tttttaacag
    16681 cgccacccag ttgcacaccg ataaaatcaa ggtttttata gactcggaag cttttgccac
    16741 aatgaaatag ctgttgtaac tgttctaaaa agagattatc cgtcgaggcc actgcaatcg
    16801 cggtgggcaa acccgcagca agctctttag caaaagtagg gccagataat actgcgagcg
    16861 ggatttcatt acctaatact tcacgggcaa catcttgtaa taagcgacca gtatgtgctt
    16921 caagaccttt tgtcgcccaa acaacacgtg catcctgacg taaaaagggt ttgatttgtt
    16981 gcagtacttc accaaacaca tggcttggga tcaccacaag aatattacgg ctcgcttcaa
    17041 tcgctttttg caaagaagct tccatatata aactatcagg aaaggaaaca tcgggcagaa
    17101 aggcttgatt acagcgcgct tgttctaatg ctgcaacgtg cttgggatca tgaccccaca
    17161 gcacaacatc gtgcccatta cgcgctaagg taatcgctaa agcggtgccg tatgaaccgg
    17221 caccgataac tgtcatagaa gcgttcatta agcttcttca actcctgagt ttgctgcatc
    17281 ctgttgctgt tgttgctgta agtagttcat aaacaacgca tcaaagttaa caggtgctaa
    17341 gtttaattgt gggaaagtac cacgacctac taagttggtg atacattcac gtgcataagg
    17401 gaataatacg ttcgggcagt atgcacctaa gcaatgtgct aattgtgtac cttcgatacc
    17461 ttca
//
