LOCUS       KY710700               12415 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus vulgaris strain PrK 39/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710700
VERSION     KY710700.1
KEYWORDS    .
SOURCE      Proteus vulgaris
  ORGANISM  Proteus vulgaris
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12415)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12415
                     /organism="Proteus vulgaris"
                     /mol_type="genomic DNA"
                     /strain="PrK 39/57"
                     /serotype="O21"
                     /db_xref="taxon:585"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..12415
                     /note="O antigen gene cluster"
     gene            7..684
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             1077..2306
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99553.1"
                     /translation="MSYTHINKNIFYLIFVQLANYIAPLVILPYLSRTIGIEGVGTVV
                     IALSLCSLALIFTDFGFAVAGPYWIAKNYNKKNKLALYMGSIFILKLIFYMVILLTYI
                     ICLNFIDFLYNQTIYFHIIILFTIFFQAFQITWFFQGIEKMKNITICTVTTKATYLIL
                     VILFINNKDDVNFTLFCFMFSNLLSATLGLYLMQKEKYYILISTPKYIWQIFKENTSF
                     FLSRAAVGIYTSASTFLVGNYSGIQQAALYSSAEKLYQGAVSLTAPVSQALYPHLAKS
                     GERKVLYKFIFILSPIMLFCGLVCIYFSDSIIIFIYGKDFLEASSLLKIFILISIISF
                     ISINFGYPAYSTIGRLDLVNKSVILGGLLQLSLILILIFLGKLNANSIVISILFTEVI
                     ILLFRVLNYLNIIRKES"
     CDS             2309..3469
                     /note="CDP-glycerol:poly(glycerophosphate)"
                     /codon_start=1
                     /transl_table=11
                     /gene="tagF"
                     /product="tagF"
                     /protein_id="AXY99554.1"
                     /translation="MNIIFRLIISFIDIFVPKNKKKISIPCINDNEWQGNCSFFSQYL
                     IKNKELLGIQINILCNKKSFLFRNPDIHLDTQYLYSLKGIWTLLTSGIVIYHHGPLAG
                     RIPLISSRRINFHINHGIHFKKVELALDSKSKEYKNISFIKTKWFCKYHSVSSNIDAL
                     SCCSYYHTYLSNIFITGIPRNDLFFINNDDLLPQEVINDINKLKKIANNRKIILYAPT
                     WRNQGGAYIFNQDEKNILEKFLINNNYIFVYAGHPYLKNRVIPQSKYIIDYNNNFSDI
                     QSVLSITDILITDYSSIWIDFLLKDKPIIGFQFDHDTYKDDRGFLFDYNEVFPGEIVF
                     DFQSLISVLKLNINDNFIKTEQFSFSKKLFHKFTDGKNCERIANSLLSIKND"
     CDS             3462..4637
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99555.1"
                     /translation="MIKYKKYNPILNKYLIILLLLITNMQTTSAFSLYFLCIFVFGLI
                     AALIVVTNHTIKLNFLISTIALILILGILTKLHADRYVNYIGLNEILYKKYILVIFWS
                     LMSLLSINIFIHTPNDIIKNSLSIIILIMSFFVILQLFSYYILNITIDFSLITGGIES
                     RSYFGNYRPSGFLPEPAVYCGHMSGLLSLYLFYNKKIDKYFILGVLSIFITQSTVGVI
                     LVSLIISIYLLSFKINKNKLFLILAFLMIILLFIAPEIINRINLFLQGTDTSNNLKVD
                     AVINFLKDDNIFIFGYGLISKDHIELPTYYEAIKDLTIFGSALSIFGIFFGIIIIILL
                     LYILTVSHVELLYKIILFIPLLKLCSPSYAFFYIYISLFFIILLDKRNKFTRLSKTL"
     CDS             4634..5743
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99556.1"
                     /translation="MKLLFVITGLGMGGAEVQLCNLADKLQSEYNNNIIIIVLSGENI
                     INIPKSKKIKIINLNIKKNPTSFIFGLIKARAIIKKFKPDIVHSHMFHANIFSRILRL
                     FCKIPVLISTAHSKNEGGKFRMFLYRATDKLATISTNVSQEAVDSFILQKAAPPEKII
                     AMYNGIDTTLFSFNEIAREKKRAEINLNDNCNLILAVGRLTKAKDYPNLLHAFSKLNL
                     SKKSKLVIIGDGEEKENLQQLACDLKISKDIIWLDIRYDVPDWMSACDLFVLSSAWEG
                     FGLVVAEAMACERLVIGTDSGGVKEVINKFGQVVPPENSIVLSSVIAEYLTLPEHKRN
                     TLGILAREHIIDTFSIEKICQKWLSLYSELLNEHE"
     CDS             5730..6773
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99557.1"
                     /translation="MNMNKKIAIVITKSEIGGAQTWALELYNILKNFFDVYLITSEKG
                     WLTEHFPIKNIFIIPELASIKKPSCIFKISKILKENNIDTVISNSANAGLYSRLAKIL
                     KPHNHIYVSHGWSCIYNGGKFKIIFCLIEKLLSFITNTILCVSERDKKNAIDIIGINK
                     NKISVIKNRISPLKRKENINERLKALFVGRLTHPKRADLFIEAAKRQPFIDFYIVGDG
                     PLLESLKSQYSDTYKNIFFLGEISNFNTYCDFDIFVLCSDSEGLPMSAIEAGSAGLPL
                     ILSDVGGCSELIYYSNNKSNGLLIKNNIDNLSETLTEMSINYMTYYKCAQNMRDKFNI
                     NSISHEYIKLIQG"
     CDS             6780..7727
                     /note="UDP-N-acetylglucosamine 4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gne"
                     /product="gne"
                     /protein_id="AXY99558.1"
                     /translation="MLTIIGGSGFIGTRLSDQLTKENIEFKIVDIAKSEKYPEKWVFG
                     DVTKPETLLEPLKGSEVIINLAAQHKDNVHPISLYYEVNVDGAKNVCDVAEQLNIKHI
                     VFTSSVAVYGFVEKETGEDGEFHPFNDYGKSKLDAEHQYEAWYKKNPSNTLVVIRPTV
                     VFGEGNRGNVYNLFRQIASGKFLMVGSGNNKKSMAYVENIAAFLKFATKIESGHHIFN
                     YIDKPDFTMNELTDIIYTALDKRHTHIKVPYAIGLLGGYCFDILSKVTGKEFPVSSIR
                     IKKFCARTQFKSNSITNTNFKAPVTLEQGIAYTVKNEFL"
     CDS             7961..9127
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99559.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFLATKELDFTATLDKEFAYKDADFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNAKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLNEFKAISDVIITNRMSSVLNDVEEKVYTRDLFGND"
     CDS             9139..9642
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99560.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(9718..10275)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXY99561.1"
                     /translation="MALTEVTFSELKACLHAEYIGDNKPFSWIRLFHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFLKKLGIYIGAKNNRKYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKDGKADIRVGNHVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYLTKVVAEIKQLSM"
     CDS             complement(10275..11096)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99562.1"
                     /translation="MSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHENLGSA
                     LSYMLANKLATQTMPAIAVREIVEEAYRSDDSMIESAAYDISAVRLRDPAVDKYSTPL
                     LYLKGFHALQAYRIAHWLWGQDRKALAVYLQNQISVTFGVDIHPAARIGHGIMLDHAT
                     GIVIGETAVVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEIGRGA
                     KIGAGSVVLHAVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDGI"
     CDS             complement(11212..11328)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="gpsA"
                     /protein_id="AXY99563.1"
                     /translation="MPITEQIYQILYQHKDAKEAALSLLGRATKDEIDSTLF"
     CDS             complement(11378..12217)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA_2"
                     /product="gpsA_2"
                     /protein_id="AXY99564.1"
                     /translation="MTVIGAGSYGTALAITLARNGHDVVLWGHDAEHVAALEQARCNQ
                     AFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARVVWAT
                     KGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTFSEEL
                     QQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITRGLAE
                     MSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEKIGQV
                     VEG"
     gene            complement(12229..12414)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     4276 a   2019 c   1947 g   4173 t
ORIGIN
        1 gaacgtatgg ttgaagcaca gcagcgttta atttctgata tttctcatga attacgaact
       61 ccattaacac gcttacaatt agccagtgca ttgttacgtc gccgcaacgg agaaagtaaa
      121 gaattagaac gcattgaaac tgagactcaa cgccttgatg ggatgattaa tgacttattg
      181 gtgctttcac gtaatcaata taaaaatgag ttgttacgag aaactgtaaa agcaaatgag
      241 ttgtgggatg atattttaga taacgctaaa tttgaggctg aacagagtaa taagacatta
      301 caagtgacaa cacctccagg tgcatggcct atttactgta acccttactc actcgctagc
      361 gcatttgaaa atattgttcg taatgcctta cgttattcaa attctcgcat tgaagttgca
      421 ttcacagagc aaaatcaagg catcaccatt attgttgatg acgatggccc aggtgttagc
      481 cctgaagaca gagagcatat cttccgaccg ttttatcgca ctgacgaagc aagagatcgc
      541 gaatcaggtg gtacaggctt aggattagcg attgttgaga cagcagtaag tcaacatcga
      601 ggccatgtta aagccgatga cagcccatta ggtgggctga gagtagagat ttggttgccg
      661 aggggaagtg ccggtaataa atagccaata gcctagatag agtaattaaa ctgttgtatc
      721 aaaatatcct gccatctatt gaagaatagg tggcatattc tttttgttag catctaatga
      781 tgcaattcag aatgatctgg ctgaatcgac cgctattccg atatttatat atctacaatc
      841 aatgctaaaa tacccagcta ttacttgacc gtctggtttg aagagtctac tctcagtgcg
      901 gtttttcgca tattgttaaa atgtgcttat ataacaaaac tcttatatta caaaaagata
      961 agggtttttc gagataagtt ttggcgctct gggggccgta aacccagtgc ggtagctttg
     1021 ttcaattatt aatatctatt tttcgatatt aataatagtc tgctggatat catattttgt
     1081 cttacacaca tatcaataaa aatatttttt atcttatttt tgtacagcta gctaattata
     1141 tagccccact tgttatacta ccttatttat ctagaaccat tggtattgaa ggtgttggta
     1201 ccgttgtaat tgctttatca ctgtgttcat tagcattaat atttacggat tttggttttg
     1261 ctgtagctgg cccttattgg atagcgaaaa attataataa aaaaaataaa ttagcattgt
     1321 atatgggctc aatatttata ttaaaattaa ttttttacat ggtaatttta ttaacatata
     1381 taatatgttt aaattttatt gattttttat acaatcaaac aatatatttc catataatta
     1441 tcttatttac tatatttttt caagcttttc aaataacttg gttctttcaa ggaatcgaga
     1501 aaatgaaaaa tattactatt tgtacagtaa cgacaaaagc cacatatctc atattagtaa
     1561 ttttatttat caataataaa gatgatgtta attttactct tttttgcttt atgtttagta
     1621 atctattgtc tgctacttta ggactttatt taatgcaaaa agaaaaatac tacatactta
     1681 tatctacacc taaatatatt tggcaaatct ttaaagaaaa tacttctttt tttctttcta
     1741 gagcagctgt tggtatatat acaagtgcaa gtacttttct tgtaggcaat tattctggta
     1801 tacaacaagc cgctttatat tctagtgcag agaagctcta tcaaggtgct gttagtttaa
     1861 ctgcccctgt aagccaagcg ttatatcctc acctggctaa aagtggtgaa agaaaagtat
     1921 tatataaatt tatttttatt ctttctccta taatgttatt ttgcggatta gtttgtattt
     1981 atttttccga ctccataata atttttattt atggtaaaga ttttctagaa gcatctagtt
     2041 tattaaaaat attcattttg atttcaatta ttagttttat tagtatcaat tttggttatc
     2101 cagcctattc aacaattgga aggcttgact tagttaataa aagtgtcatt ttaggtggtc
     2161 ttttacaatt atcattaatt ttaatattaa tttttctagg taagctcaac gcaaattcaa
     2221 ttgttatttc gatacttttt actgaagtta ttattctctt atttagagta ttgaattatt
     2281 taaacattat aagaaaagaa agttaactat gaatataatt tttagattaa taatttcctt
     2341 tatagatatt ttcgttccta aaaacaaaaa aaaaatatct ataccgtgca taaatgataa
     2401 tgaatggcaa ggcaattgtt cattcttttc tcaatatctc attaagaata aagaattgct
     2461 aggtattcaa ataaatatac tctgtaataa aaaaagtttt ttatttagaa atccagatat
     2521 tcatttagat acacaatatt tatattcttt aaaaggaata tggacgttac taacatcagg
     2581 tattgtcatt tatcaccatg gtccattagc aggtagaata ccgctaatat cttcgcgtag
     2641 aataaatttt catatcaacc atggtataca ttttaaaaaa gtagaattag ccttagattc
     2701 aaaatctaaa gaatacaaaa atatatcttt cattaaaaca aagtggtttt gtaaatatca
     2761 ttctgtttca tcaaatatag atgcattatc ctgttgttct tattatcaca cttacttatc
     2821 aaacatattt ataactggta ttccaagaaa tgatttattc tttataaata acgatgattt
     2881 attaccacaa gaggttatca atgatattaa taaactaaaa aaaatagcca ataatagaaa
     2941 aattattctt tatgctccaa cctggagaaa tcaaggagga gcatacatct ttaatcaaga
     3001 tgaaaaaaat atccttgaaa agttcttaat caacaataat tatatatttg tctatgctgg
     3061 acatccttat ttaaaaaatc gagttattcc tcaatctaaa tatataattg attacaataa
     3121 taattttagc gacattcaat ctgtacttag cattacagat attttaatta ctgattactc
     3181 tagtatatgg atagattttt tgctaaaaga taaacccatt attggttttc aatttgatca
     3241 tgacacgtat aaagatgata gaggtttttt atttgattat aatgaagtct ttcctggaga
     3301 aattgttttt gattttcaat ctttaatttc agtattaaaa cttaatataa atgataattt
     3361 tataaaaaca gaacaatttt ctttttctaa aaaattattt cataaattca ctgatgggaa
     3421 aaattgtgaa agaattgcaa actcactatt aagtataaaa aatgattaaa tataaaaaat
     3481 ataatcctat attaaataaa tacttgataa ttttattgtt acttattact aatatgcaga
     3541 caacgagtgc tttttctcta tattttctat gcatatttgt atttgggtta attgctgcat
     3601 taatagttgt aaccaaccat actataaaat taaatttcct tatatctact atagcattaa
     3661 tactcatatt aggaatacta actaaacttc atgcagatag atatgtcaat tacattgggc
     3721 taaatgagat tttatataaa aaatatattt tggttatttt ctggagttta atgtcgttat
     3781 tatccataaa tatatttata catactccaa atgatataat aaaaaattca ttatcaataa
     3841 tcatattaat aatgagtttt tttgttattc ttcaattgtt ctcttattac atcttaaata
     3901 ttactattga cttttcttta ataactggag gtatagaatc tagaagctat tttgggaatt
     3961 atcgccctag tggtttttta cctgaaccag cagtttattg tgggcatatg agtggcttgc
     4021 tatctttata cttattttat aataaaaaaa ttgataaata ttttatttta ggtgttttat
     4081 caatatttat aactcaatcc acagtaggtg taattttagt aagtttaatt atttcaatat
     4141 atttactctc atttaaaata aataagaata aattattctt gatattagct ttcttaatga
     4201 tcattctgct atttattgca ccagaaataa ttaatagaat caatttattt ttacaaggaa
     4261 cagatactag taataattta aaagttgatg cggtaatcaa ttttcttaaa gacgataata
     4321 tatttatatt tggatatggt ttaatctcaa aagatcatat tgaattacca acatattatg
     4381 aagcaattaa ggatttaaca atctttggtt ctgcactttc tatatttggt attttttttg
     4441 gaataataat aattatatta ttattatata ttttgacagt gtcacatgtt gaattattat
     4501 acaagataat attattcatt ccattattaa aactatgtag tccaagctat gcttttttct
     4561 atatatatat atcgctcttt tttattatac ttcttgataa gagaaataaa tttacaaggt
     4621 tatcaaaaac attatgaaat tattatttgt aattactggc ttaggcatgg gtggagctga
     4681 agtacaatta tgtaatttag cagataagct tcagtcagaa tataataata atattattat
     4741 tatagttcta tctggtgaaa atattataaa cataccaaaa tcaaaaaaaa taaaaataat
     4801 caatttaaat ataaaaaaaa atccaacctc tttcatcttt ggtcttataa aagctagagc
     4861 aataataaag aaatttaaac cagatatagt tcatagtcat atgtttcatg ctaatatatt
     4921 ttctcgtata ctacgtttat tttgcaaaat tccagtatta atatctacag cacatagtaa
     4981 aaatgaaggt ggaaagtttc gaatgtttct ttatcgggct actgataaat tagccacaat
     5041 ctcaactaac gtaagtcaag aagcagtaga ctcatttatt ttacaaaaag cagctcctcc
     5101 tgaaaaaata attgcaatgt ataatggtat agatacaact cttttctcat ttaatgaaat
     5161 agccagagaa aaaaaacgag ctgaaattaa tttaaatgat aactgtaatc ttatattagc
     5221 agtaggtaga ttaacaaaag ctaaagatta ccctaattta ttacatgctt tttctaaatt
     5281 aaatttatct aaaaaatcta aattagtaat cataggtgat ggcgaagaga aagaaaattt
     5341 acaacaatta gcatgcgacc taaaaatatc taaagatatt atatggttag acattaggta
     5401 cgatgttcca gattggatgt ctgcatgtga tctatttgta ttatcttcag cttgggaagg
     5461 ctttgggttg gttgtagcag aagcaatggc ttgtgaacgc ttagttatag gaactgattc
     5521 tggtggtgta aaagaagtta tcaataaatt tggacaagta gttcctcctg aaaattctat
     5581 tgttttatca tctgtaatag ctgagtattt gactttacct gaacataaga ggaatacttt
     5641 aggtatattg gcacgagaac atattataga tacattctct attgagaaaa tatgtcaaaa
     5701 atggctaagt ctttacagtg agcttttaaa tgaacatgaa taaaaaaata gctatcgtta
     5761 taaccaagtc tgaaataggc ggcgcacaaa cttgggctct tgaattatat aatatattaa
     5821 aaaatttttt tgacgtatat ttaataacat cagaaaaagg ttggctaaca gagcattttc
     5881 caataaaaaa tatatttatt attccagaat tagcgagtat aaaaaaacca tcttgtattt
     5941 ttaaaatatc aaaaatatta aaagaaaata atattgatac cgtcatttct aattcagcaa
     6001 atgcaggatt atactcgcga ttagctaaaa tattaaaacc ccataatcat atatatgttt
     6061 ctcatggatg gtcatgcata tataatggag gtaaatttaa aataattttt tgtttaattg
     6121 aaaaactatt atcctttatt actaatacaa ttctttgtgt ttctgaaagg gataaaaaaa
     6181 atgctattga tattataggt ataaataaaa ataaaatatc agttataaaa aacagaatct
     6241 cacctttaaa aagaaaagaa aatataaatg aaagattaaa agctcttttt gtaggaagat
     6301 taactcatcc taaaagggct gatttattta ttgaggcagc aaaaagacaa cctttcattg
     6361 atttctatat agtaggtgat ggtcctttac ttgaaagttt aaagtcacaa tattctgata
     6421 cttataaaaa tatatttttt ttaggtgaga taagtaattt taatacctat tgtgattttg
     6481 atatttttgt tctttgttct gacagtgaag gtttacctat gtcagctata gaagcaggtt
     6541 ctgccgggtt accattaatt ctaagtgatg taggtggatg ctctgaactt atatattatt
     6601 cgaacaataa aagtaatgga ttattaataa aaaacaatat agataattta tctgaaacgt
     6661 taacagaaat gtcaataaat tatatgactt attataaatg tgcacaaaat atgagagata
     6721 aatttaatat taactcaatc tctcatgaat atataaaatt aatccaagga taattattta
     6781 tgctaacaat aatcggtggt tcaggtttta ttggcacacg cctttcagat caattaacta
     6841 aagaaaatat tgaatttaaa attgtagata ttgctaaaag tgaaaaatat cctgaaaaat
     6901 gggtatttgg tgatgtaaca aaaccagaga cattacttga accattaaag ggttctgaag
     6961 ttattatcaa cttagcagca caacataaag ataatgttca tcctattagt ttatattatg
     7021 aagttaatgt agatggagct aaaaatgttt gtgatgttgc tgaacagtta aatattaaac
     7081 atatcgtatt tacttcatct gttgctgtat atggatttgt tgaaaaagaa acgggtgaag
     7141 atggtgagtt tcatccattt aacgattatg gtaaatctaa acttgatgca gaacatcaat
     7201 atgaagcatg gtataagaaa aacccttcaa atactttagt agtaatcaga ccaacggttg
     7261 tttttggtga aggaaataga ggcaacgttt ataatttatt ccgtcaaata gcgtcgggta
     7321 aatttttaat ggtgggttca ggtaataata aaaaatctat ggcttatgta gaaaacatcg
     7381 ctgctttctt aaaatttgct actaaaatag aatctggtca tcatatattt aattatattg
     7441 ataaacctga ttttactatg aatgaattga cagatatcat ttatacagct ttagataaac
     7501 gacataccca cattaaagtt ccttatgcta ttggattact tggtggatat tgttttgata
     7561 ttctttctaa agttacaggt aaagaatttc ctgttagtag tattcgtatt aaaaaattct
     7621 gtgctcgtac tcaatttaaa tcaaatagta ttactaatac taactttaaa gctccagtta
     7681 ctcttgagca aggtattgct tatacagtta aaaatgaatt tttataatag atcaatttta
     7741 aatagtcttt atattaacaa ataattaaaa tacaccctac acttctctac ttattttcta
     7801 ataaaaaatt aattttatat taatttaata agtatatgat ccttatgatt gctaaatata
     7861 ctataccttt actaaagagt gagatttaac actaaaatat ctatttttat tctagtatag
     7921 tgaaattata taaatacata tactttagat aagagaaacc atgaaaatca ccatctcagg
     7981 taccggctat gtcggtctat ctaacggaat acttctttcc caaaacaaca aagttgttgc
     8041 gttagatatc aataaagaaa aagttgaatt actgaataaa aaaatctctc caattacaga
     8101 taaagaaatt gaagaatttt tagcaaccaa agagctcgat tttactgcaa ccttagataa
     8161 agagttcgct tataaagatg cggattttgt tatcattgca acacctaccg attacgatcc
     8221 taaaacaaat tactttaata cctcctctgt agagtctgtt atccgtgatg ttttagaata
     8281 taatccaaac acgacaatga ttataaaatc aaccattcct gttggtttta cgaaagaaat
     8341 gagagaaaaa tataatacgg ataatattat tttctctcct gaattcttac gtgaaggtcg
     8401 tgcactttac gataatttat acccatcgcg tattgttatt ggtgaacaat cagaaagagc
     8461 acaaaaattt gcagaccttt tacaacaagg tgcattaaag aaagatattg atgttctgtt
     8521 cactgactca acagaagcag aagccattaa attatttgcc aatacttatt tagcactacg
     8581 tgttgcttac tttaatgaat tagatagcta cgctcaagct tatggcttaa atgcaaaaca
     8641 aatcatcgaa ggcgtctgct tagatccacg tatcggtaat cattataata acccatcatt
     8701 cggttatggc ggttactgct taccaaaaga tactaaacag ctgttagcaa actacgactc
     8761 ggtgcctaat aatattatta gtgcaatcgt cgaagctaac cgcacacgta aagactttat
     8821 tgccgactca attatcgcta aatcaccaaa aatcgtaggt gtttatcgtt taattatgaa
     8881 atctggctct gataatttcc gttcttcttc tatccaaggc attatgaaac gcattaaagc
     8941 caaagggatt gaggtggtaa tctatgagcc agagatgaaa gaagagactt tctttaactc
     9001 aaaattaatt cgtgatttaa atgagtttaa agcaatctct gatgtcatta ttactaatag
     9061 aatgtcatct gtactaaacg acgttgaaga aaaagtttat actcgtgacc tctttggtaa
     9121 cgactaaatc gattttatat gctcaatatc gtcttatttg aacccgaaat tccaccgaat
     9181 acgggtaata ttatccgcct gtgtgctaac acaggctttc atcttcattt gatccaacct
     9241 ttgggtttta cttgggatga taaacgtctt cgtcgtgcag gacttgatta tcgtgaattt
     9301 gctgatatta aacagcatca cgattactat gcttttttag aaagtgaagg tttaaaccct
     9361 gataatgcac aatcaccaac gggtgcgcgt gtatttgcgt taacgacaaa aggtacacca
     9421 gcacatagca atgtaagcta tcaagatgga gattatctcc tttttggccc tgaaacccgt
     9481 ggtttgccac cttatgtgtt agataatatg ccaacacagc agaaaatcag agttccaatg
     9541 cttgctgata gtcgcagtat gaacctgtca aataccgttg cagttgtggt ttttgaagca
     9601 tggcgtcaac ttggttatcc tggtgcgtta ttaagagact gaaatgcaaa aagcgctgat
     9661 cataaataat gagcaagcgc ttttttaatt tcaaatgact aaaaacttaa tttattatta
     9721 cattgagagt tgttttattt cagcaacaac cttcgtgaga tacctacacc cttcccctac
     9781 atccttcaat accaatgcgt gagcaccaat cactgcatta tcaccaatct ctatctcacc
     9841 gccgataatc gaagaattac aaccaatata aacgtggttg ccgactcgaa tatctgcttt
     9901 accatccttt tcttttactc cgatggtcac accttgcatt aacgtcacat tctcacctaa
     9961 ttgacagtaa ttagtcagca caataccgat aggatgtggt aatgaaaggc cttttcctat
    10021 gcgcgttctt aatgagatat cattgcaata tttacgattg tttttagcac caatgtaaat
    10081 gcctaatttt ttgagaaaac gatttttaga gaaataaaaa aattgagcga cacgccacca
    10141 aaacagataa cgtgaacgtt ggcagaaaaa gacgcgatga aaaagtctta tccaactaaa
    10201 aggcttgtta tcaccgatat attcagcgtg taaacaagcc ttaagttcag aaaatgtaac
    10261 ttctgttaat gccattaaat gccgtcacca cattcaaaac caggtactac accattaaag
    10321 tgttgatcca tatcgagtga aggtttatca ctcgtcggct taccaacaat gcgagcagga
    10381 acccctgcaa ccgtggtatg tgcaggcact gcatgtaata cgacagatcc cgcaccaatt
    10441 ttagcaccgc gaccaatctc aatattgccg agtattttag cacccgcacc aatcatcaca
    10501 ccttcacgca ctttaggatg acgatcacca caggttttac ctgttccgcc taaagtcaca
    10561 gattgtaaaa tagaaacatc attctcaaca acagctgtct caccaattac aatgcctgtt
    10621 gcgtgatcaa gcataatacc gtggccaata cgtgcagctg ggtgaatatc gacaccaaac
    10681 gtaacagaaa tctggttttg taggtaaaca gcaagcgctt tgcgatcttg tccccataac
    10741 caatgtgcta tgcgataagc ctgcaacgca tgaaagcctt ttaaataaag caacggtgtg
    10801 gagtatttat caactgcggg gtcgcgtaaa cgaacagctg aaatatcata agccgcagat
    10861 tcgatcatgg aatcatcgct acgatacgct tcttcaacaa tctcacgaac ggcaatcgca
    10921 ggcattgtct gtgtcgccag cttattcgct agcatataac ttaaggcgct acctaagttt
    10981 tcatgtttga gtaatgtcgc atggaagaaa ctcgccaaaa tcggctcgca atcagccaaa
    11041 catcttgctt catttttaat atgatcccag actcttttta gttcttctag cgacatactc
    11101 actctcattc tttttacatt gaaacctaag taacaactta agcttcttat gttgaaaacc
    11161 gaaacagaca ccttaaacca atgcgttaag tagcgattat ttaaaataga cttagaacag
    11221 agtgctgtct atttcatctt ttgttgcgcg acctaataaa gatagtgccg cctctttcgc
    11281 gtctttatgt tggtataaaa tttggtagat ctgctcagtg ataggcattt caacaccgac
    11341 ttgttctgct aaggcgcgaa cttctttagt gttccgatta accttcaacc acctgcccga
    11401 ttttctcttg ggcagtatca acatcaagcc cttggcctaa catcatacca aatcggcgat
    11461 tacgggattg gttatcggta caagttaaaa cgagatcgcc taacccggcc attcccataa
    11521 acgtcgcagc atctgcacct aatgctttac ctaaacggct catttcagca agtccgcggg
    11581 taattagtgc tgtacgtgca ttcgcgccaa accccatacc atcagacatt ccagcaccaa
    11641 tagcaatcac gttttttacc gcgccaccta attgcacacc gataaagtca ggatttttat
    11701 acacacggaa acttttacca caatggaaca actgttgaag ctcttcagaa aacgtattat
    11761 ctgttgatgc aacagatatc gcagtaggca gacctgctgc aagctctttg gcaaaagtag
    11821 gaccagataa aactgcgagc gggatttcat tacctaatac ttcacgcgcc acatcttgta
    11881 acaagcgacc agtatgagcc tcaagtcctt ttgtcgccca aacaacacgt gcatcctgac
    11941 gtaaaaaggg tttgatctgc tgtaatacgt caccaaaaac atggcttggg atcacaacca
    12001 gaatattacg gctagcttca atggcttttt gcaaagaagc ttccatgtat aaactatcag
    12061 gaaaagaaac atcgggcaga aatgcctgat tacaacgtgc ttgttctaac gccgcaacat
    12121 gttcagcatc atgaccccaa agtacaacat catggccatt gcgtgctaag gtaatcgcta
    12181 aagcggtgcc gtatgaaccg gcaccgataa ctgtcataga agcgttcatt aagcttcttc
    12241 agccccttca ttagcagcat cagtttgttg ctgttgtaaa tagttcataa acaacgcatc
    12301 aaagttaact ggtgctaagt tcagttgtgg gaaagtacca cggctgacta agttagtgat
    12361 acattcacga gcataaggga acagaacgtt aaggcagtat gcacctaagc aatgt
//
