LOCUS       GU254061               14554 bp    DNA     linear   BCT 14-AUG-2010
DEFINITION  Proteus vulgaris strain CCUG 19017 O antigen gene cluster, complete
            sequence; and WenH (wenH), CysE (cysE), and GpsA (gpsA) genes,
            complete cds.
ACCESSION   GU254061
VERSION     GU254061.1
KEYWORDS    .
SOURCE      Proteus vulgaris
  ORGANISM  Proteus vulgaris
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 14554)
  AUTHORS   Wang,Q., Torzewska,A., Ruan,X., Wang,X., Rozalski,A., Shao,Z.,
            Guo,X., Zhou,H., Feng,L. and Wang,L.
  TITLE     Molecular and genetic analyses of the putative Proteus O antigen
            gene locus
  JOURNAL   Appl. Environ. Microbiol. 76 (16), 5471-5478 (2010)
   PUBMED   20581173
REFERENCE   2  (bases 1 to 14554)
  AUTHORS   Wang,Q., Torzewska,A., Ruan,X., Rozalski,A., Shao,Z., Guo,X.,
            Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..14554
                     /organism="Proteus vulgaris"
                     /mol_type="genomic DNA"
                     /strain="CCUG 19017"
                     /db_xref="taxon:585"
                     /note="serogroup: O23a,23c"
     gene            530..1423
                     /gene="wenA"
     CDS             530..1423
                     /gene="wenA"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32305.1"
                     /translation="MNKYLKILRNKLRYKYCDVFPELVTKSIYKERIKKTLNLDSPQT
                     FNEKLQWLKLNLYKNNPLVTKCADKYAVREYIKDCGCQEILNEIHQVWDQPDEINFSE
                     LPNKFVLKCNHGAGYNIICRDKNKISSGDIKQTLTTWLNEDYWRLAVEFVYKDVPKKI
                     ICEKFIETENNELPYDYKLFCFHGKPLFVMICTERESQHPKFYFVNKEWELLPYGIDY
                     KNSEVSTILDKPQNYQKLFEYAEKLSKPFPFVRVDLYLNSGIIHFGELTFIPSAGMDK
                     ELNNEETKNVDLIFGNYLKLN"
     misc_feature    530..11837
                     /note="O antigen gene cluster"
     gene            complement(1434..2423)
                     /gene="wenB"
     CDS             complement(1434..2423)
                     /gene="wenB"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32306.1"
                     /translation="MKNTPKISVLMSVYNDSIHLKEAIDSILNQTFNDFEFIIIDDGS
                     TDLSIDILKGYEEKDDRVKVYTNAFNIGLAKSLNKGFLLASGDYIARMDADDYSYPTR
                     LQKQFEFMENHKNIMVCGTSMEIYEYNNNFMILPTKHNAIAAGLIFGTNFYHPTVMFR
                     TDFIKQNKIMYPEDYKYAQDYGLWVNLLKLKQENIFSNLEEALLKYRIHPEKNREKYF
                     STQTSFAIKAQINALSLFNIEVSEDILYNLNFSTEDLSLYDIKKCNVILDKIKTKNKE
                     LCPYFYKDLEVAIIRKKVSLFRRCEKKSLFYPYMKVKARLTYLINRKMIKALN"
     gene            3057..4169
                     /gene="wenC"
     CDS             3057..4169
                     /gene="wenC"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32307.1"
                     /translation="MKKIAITSNTSWYLYNFRKNTILSLIKSGFEVYTISPFDEHSKK
                     LESLGAKHINIKIDSASKNPFVDLYTLISFYRIFNKFKFNAILNFTPKNNIYGTLAAK
                     LNQIETINNIAGLGTLFISNNISSRLARNLYRYSQKYANKIFFQNNDDLTLFLEKNYV
                     KKSQIDRLPGSGVDLSRFTLSLSENKSQLRFLLIARMLYEKGITFYVDAARILKEKYG
                     DRVEFCLLGFVGVNNPSAITQEQMNNWISEGFINYLGTSDFVEYEIAQADCIVLPSFY
                     REGVPKTLLEAGAMGKPIITTDNVGCRETVNHGFNGYLCQPKSVSSLVDSLDLFINLP
                     YEDKLEMGKNSRKKIESEFDEQIVIKKYLDALEEIL"
     gene            4188..5354
                     /gene="ugd"
     CDS             4188..5354
                     /gene="ugd"
                     /note="UDP-Glc-6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="Ugd"
                     /protein_id="ADL32308.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFLAAKELDFTATLDKEFAYKDADFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTNNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDILFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYNLNAKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKVVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMQEETFFNSRLVRDLNEFKSISDVIITNRMSSVLNDVEEKVYTRDLFGND"
     gene            5380..6390
                     /gene="gla"
     CDS             5380..6390
                     /gene="gla"
                     /note="nucleotide sugar epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gla"
                     /protein_id="ADL32309.1"
                     /translation="MKYLVTGAAGFIGFHLIKKLIQQGETVVGIDNLNDYYDVSLKEE
                     RLNILNQLNNFSFSLIDLADREKIASLFETEKFDRVIHLAAQAGVRYSLINPFSYADS
                     NLTGFLTILEGCRHNNVKHLVYASSSSVYGLNDELPFSPHDQANHPVSLYAATKKANE
                     LMAHSYSHLYGIPTTGLRFFTVYGPWGRPDMALFKFTKAIINDDPIDIYNNGQMRRDF
                     TYVEDIVEGVARIADVIPAPQQDWKVSTGTPANSSAPYKVYNIGNGSPVNLMDYISAL
                     ETHLGKTADKNMLPMQPGDVYTTWADTEDLFKATGYKPQTSVNEGVKQFVDWYKNYYQ
                     VK"
     gene            6394..6894
                     /gene="wenD"
     CDS             6394..6894
                     /gene="wenD"
                     /note="methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WenD"
                     /protein_id="ADL32310.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLK"
     gene            6900..7364
                     /gene="wenE"
     CDS             6900..7364
                     /gene="wenE"
                     /note="O-acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WenE"
                     /protein_id="ADL32311.1"
                     /translation="MKTFFLKKLSSLIVKIYVFIFYNELIYNKNIIYFGFFTIKNIKK
                     LSIKGRVNINDHVYINAKGGITLGDKVTLSAGSKLISTGLLINNNGITTEHIDKEIII
                     SDNVQIGAGAIVLAGVSICENVVVGAGSIVTRDIEKSGVYVGSPAKCIRLFK"
     gene            7372..8598
                     /gene="wzx"
     CDS             7372..8598
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ADL32312.1"
                     /translation="MIIRNVFYLQLIKAIELLIPLIAIPIVIKYLGISNYGIIALLQA
                     ISAYIILIGEWGYNANATREFSSDISAFEKALLYIEIQKVKIVFCILIFICLILLKFI
                     IELTYLQISILFVWISTSILQCKWYFQATYKLINLVIISLLSKIIYIVYVFLFVKYNT
                     KIEEYLLFLILPNIILFIYSNYYIIKKLSINKIKNKRCFSLSTKSKSLLIEGWHLTSM
                     RLITGAINPIALIIAKSIYGIEFVGFIGVAQRIVSASVSILTPIIDVTFPKMNYFYLN
                     NKNKFYKFINIILLFFIFIQISGIFTYTILDQYIFYILNINYSKKSSLILSLYLLIIF
                     FSLSNSFLVHMLVIMKRSKYISPFSLLSGILTISFMLSIERFNHSQYNFVFSILLGQL
                     FLFISVLYLKGKNNIS"
     gene            8595..9698
                     /gene="wenF"
     CDS             8595..9698
                     /gene="wenF"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32313.1"
                     /translation="MKNILILSNSLSNGGGERIACNLANGLKENKYKVYFYIIENNIS
                     YTLHEDIILIKNPININLPFIGKIINNFSATIHLFFILKKEKINTIISHLFRANYINI
                     FLSLFKKHKSIVVTHGSILKYSNNNLKSKINIFLIKNTFKHATKKVFLTQRMLNDYLP
                     IIGEKNNFVIPNCYNLDNILELSQAIVNDSSFFPSEYFVFVGRFHPVKNISFLLDTFK
                     TTNYKLLLIGDGDLYKNYKEKYQSNNIKFLGNKENPYPYIKNAKATILTSISEGFPNV
                     LIESLYLGVPIISSDCRTGPREILNINGEIKKGDILYNKLGVLYNINDYQGLLNSLIY
                     ISKSDKNHDKNLMTYSMKKYNIPSIVKEYINII"
     gene            9704..10900
                     /gene="wzy"
     CDS             9704..10900
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ADL32314.1"
                     /translation="MIYNKLIPLWNNKGKYRELYSFFPITCVLIIPIFYTFIIFPFYY
                     QEKLYQLFTIENSIFWISVSLVLLITSKLIYSRKRIKQYFFSKNTALIFAFIYVGIVF
                     FYIILNFIIIGFNEFYSGCHYCGEVLGGFELLRIIIFDLGTVTILLLPICLIIFRKNK
                     IFLSIHIITTLLILTYYYIGSYRNQLLPIIFLFFLIFISNYRSFCKKIFFILFLFLSP
                     IAAISMAFYHYFKDSESPLTLYDYFSMNEFYSNYNNFIIYESGFSLDLPFSGSSYLGP
                     FLHKLNTLIDTGFKTSASQMAQYMDTGIGYGFSPLLEALLNFGDLFLIGAIIVAFTIY
                     ISITLIILSKNDLISTIYFSLFVFFIFNINRVDFTSAFNIYFHKLLLIWFFTFFTNTH
                     ISNEKR"
     gene            10887..11837
                     /gene="wenG"
     CDS             10887..11837
                     /gene="wenG"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32315.1"
                     /translation="MKNDKISVLLSIYNEIDEWVILSINSIIDQNTYDKFKIQLVIVI
                     DNPNKCEHYKNIINNLEKKNIEIVIIENSINLGLANSLNKAFEYSDGDFIARLDADDI
                     AYPERLYAQLKYYKLNSFALIGTGINRIDEQGNFISTSLLPKDFLKLKRTLGYKSICY
                     HPTWFMSRNIFEKMQGYRKYPNSQDFDFISRLIDESYTISNIEEPLVMYRINTQSLSF
                     KNSLRQRKCQYHIIKMNKNRKKHGIDNYNEQKMLDYIKTNKIIDALHFKSQLLLVKAL
                     RMNKLNPCKYLLLLCSTLLSPYQTKHILRMSYFKIKNYFI"
     gene            complement(11978..12538)
                     /gene="wenH"
     CDS             complement(11978..12538)
                     /gene="wenH"
                     /note="serine acetlyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WenH"
                     /protein_id="ADL32316.1"
                     /translation="MALVEVTLSELKACLHAEYIGDNKPFSWIRLFHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFLKKLGIRIGAKNNRKYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKDGKADIRVGNHVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGDGCRYITKVVAEIRQLSES"
     gene            complement(12538..13371)
                     /gene="cysE"
     CDS             complement(12538..13371)
                     /gene="cysE"
                     /note="serine acetlyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="CysE"
                     /protein_id="ADL32317.1"
                     /translation="MRVSMSLEELKKVWDHIKSEAKCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDASMIECAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWNQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     gene            complement(13458..14474)
                     /gene="gpsA"
     CDS             complement(13458..14474)
                     /gene="gpsA"
                     /note="glycerol-3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="GpsA"
                     /protein_id="ADL32318.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGNVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     SNLF"
BASE COUNT     5106 a   2397 c   2166 g   4885 t
ORIGIN
        1 gagttattac gtgaaacggt aaaagccaac gagctatggg atgacatttt agataacgct
       61 aaatttgaag ctgaacaaag taataagaca ctgcaagtca caacgcctcc gggagcatgg
      121 cctatttact gcaatccata ttctctggcg agtgcatttg aaaatattgt tcgtaatgcc
      181 ctgcgttatt caaattctcg cattgaagtt gcttttactg aacaaaatca aggtatcacg
      241 attattgttg atgatgatgg cccaggtgtt agccctgaag atagagaaca tatcttccga
      301 ccgttttatc gcactgacga agcaagagat cgtgaatcag gtggcacagg cttaggatta
      361 gccattgttg aaacagcagt aagccaacat agaggccatg ttaaagctga tgatagccca
      421 ttaggagggc tgagggttga gatttggttg ccaaaaacaa aatgattcta tctaaaaata
      481 ttttttagtt ttagaatgcc agatagctaa actttagttg ataattatta tgaataaata
      541 cctgaaaatt ttaagaaata aattacgtta taaatattgc gatgtttttc cagaattagt
      601 cactaaatca atttataaag aaagaataaa gaaaactcta aatttagatt ctccacaaac
      661 atttaatgaa aaattacaat ggctaaagtt aaatttatat aaaaacaatc cactggtcac
      721 aaagtgtgca gataaatacg ctgttcgaga atatattaag gattgtggtt gccaagaaat
      781 acttaatgaa attcatcaag tatgggatca acctgacgaa attaatttct ctgagttacc
      841 taataaattt gtactaaagt gcaatcatgg tgccggttat aatatcattt gtagagataa
      901 aaataagata tcttcgggtg atattaaaca gacattaacg acttggttaa atgaagatta
      961 ttggcgatta gctgttgaat ttgtttataa agatgtccct aaaaaaataa tttgtgaaaa
     1021 gttcatagaa acagaaaata atgaacttcc gtatgattac aaattatttt gttttcatgg
     1081 taaacctcta tttgtcatga tatgcaccga aagagagtca caacacccca aattttattt
     1141 tgttaataaa gagtgggaat tattacctta tggtattgac tataaaaata gtgaagtttc
     1201 aactatttta gataagcctc aaaactatca aaaactcttt gaatatgctg aaaaactatc
     1261 caaaccattt ccttttgtta gagtcgatct atatctaaat agcggtataa ttcattttgg
     1321 agaattaacc tttatcccgt cagcggggat ggacaaagaa ttaaataatg aagaaaccaa
     1381 aaatgttgat ctaatatttg gaaattacct aaaactaaac taagggttcc cccttagttt
     1441 agagccttaa tcatttttct atttatcaaa taagtaagcc ttgctttaac cttcatataa
     1501 ggataaaaaa ggcttttttt ctcacagcgt ctaaacaatg atactttttt tctaataata
     1561 gcaacttcaa gatctttata aaaataaggg catagctctt tatttttagt ttttattttg
     1621 tccaaaatca cattacactt ttttatatca tataaactta aatcttcggt cgagaaattt
     1681 aagttatata atatatcttc acttacttct atgttaaata aacttaatgc attaatctgt
     1741 gcttttatag caaatgaggt ttgtgtacta aaatattttt ccctattttt ttctggatga
     1801 attctatatt ttaataatgc ctcttctaaa ttagaaaaaa tattttcttg tttgagtttt
     1861 aataaattaa cccataaacc ataatcttga gcatatttgt aatcttcagg atacataatt
     1921 ttattctgtt taataaaatc agtcctgaac ataactgtag gatgataaaa attagttcca
     1981 aaaattaacc cagcagctat tgcattgtgc tttgttggaa gaatcatgaa gttgttattg
     2041 tattcataga tttccatcga tgtaccacaa accataatat ttttatggtt ttccataaac
     2101 tcaaactgct tttgaagacg agttgggtat gagtaatcat ctgcatccat tcgagcaata
     2161 tagtccccag aagcaagtaa aaaaccctta ttcaaagact tggctaaccc gatattaaat
     2221 gcattagtat atacttttac tctatcatct ttttcttcat aaccttttaa tatatctata
     2281 gataaatcag tagaaccatc atcaatgatg atgaattcaa aatcattaaa agtctgattc
     2341 aaaatagaat ctatagcttc cttcaaatgt attgaatcat tatatactga catcaacaca
     2401 ctgattttag gggtgttttt cattttaagc ttaaaaattt cttcctattg taatggaata
     2461 ctataaagga agagatgaga ttagtgaata ccacaatgat ttgaattttg aagaggttat
     2521 tacaatagca tgggagcaac acattcaagt aaacaataat ccataaagta ggctataaac
     2581 aaaaatttta ccataagaaa caaacgtaaa gtatgtacag cccaatagag taattaaatt
     2641 gttgcatcaa aatatcctgc catccattaa ggaataagtg gcatattcct tttgttagcg
     2701 cctaacgatg taattcagaa tgatctggct gaatggagcg ctattccagt atttatatat
     2761 cctcaatcaa tgctaaaata cccagctatt acttgaccgt ctagtttgaa gagtctactc
     2821 tcagtgcggt tttcagcata tagttaaagt atgcttatat aatgaaaccc ttacattaca
     2881 tacatataag ggtgttttaa gataaatcct ggcgctctgg gggccgtaaa cctagtgcgg
     2941 tagctttgaa aataattaat tgactaatat tggtctttca tatccccaaa cacctaaatt
     3001 aagcaaatac acattcttta aagacttgta taatatgaat aatcttttaa ataattatga
     3061 aaaaaattgc aataacatca aacactagct ggtatttata taactttaga aaaaacacca
     3121 ttctttcatt aattaaaagt ggctttgaag tttatacaat atctcctttt gatgaacatt
     3181 ctaaaaaact agaaagttta ggcgctaaac atataaatat aaaaattgat tctgcaagta
     3241 aaaatccatt tgttgatctc tacacactaa tttctttcta tagaatattt aacaaattta
     3301 aatttaatgc aattttaaat ttcactccaa aaaataatat ctatggaaca ctggctgcaa
     3361 aattaaatca gatagagaca attaataata ttgctggatt aggaactctt tttataagta
     3421 acaacatcag ttctagactt gcaagaaacc tatatcgtta tagtcagaaa tatgctaaca
     3481 aaatattttt ccaaaacaac gatgatttaa ctctattttt ggaaaaaaat tatgttaaaa
     3541 aatcacagat agataggctt cctggttctg gggttgattt atcacgtttc actctttcat
     3601 tatctgaaaa taaaagccag ttacgatttc ttttaattgc acgaatgcta tatgaaaaag
     3661 gcattacttt ttatgttgat gcagctcgta ttttaaaaga aaaatacggt gatagagtag
     3721 aattttgtct tttaggtttc gttggtgtta acaatccatc agctataacc caagaacaaa
     3781 tgaataactg gatttctgaa ggttttatta attacttagg tacttctgat tttgttgaat
     3841 atgaaatagc tcaagctgat tgtattgtat tgccttcttt ctatcgagaa ggtgttccaa
     3901 aaacattact agaagcagga gccatgggaa aaccaataat tactacagat aatgttggtt
     3961 gtcgtgaaac tgttaatcat gggttcaatg gttacctatg ccaaccgaaa tctgtatcta
     4021 gtttagttga ctcacttgat ttatttatta atttacctta cgaagataaa ctagaaatgg
     4081 gtaaaaatag cagaaaaaaa atcgaatctg agtttgatga gcaaattgtt attaagaaat
     4141 atttggatgc tttagaagaa attttataag attaggatta agaaaaaatg aaaatcacca
     4201 tctccggtac cggctatgtc ggcctatcta acggaatact tctttcccaa aacaacaaag
     4261 ttgttgcgtt agatatcaat aaagaaaaag ttgaattact gaacaaaaaa atctctccaa
     4321 ttacagacaa agaaattgaa gagtttttag cagccaaaga gctcgacttt acagcaacct
     4381 tagataaaga atttgcttat aaagatgctg acttcgttat cattgccaca ccgaccgatt
     4441 acgatcctaa aacaaactac ttcaacacct cttctgtaga gtctgttatt cgtgatgttt
     4501 tagaatataa tccaaacacg acaatgatta ttaaatcgac cattcctgtt ggttttacta
     4561 aagaaatgag agaaaaatat aatacgaata atattatctt ttctcctgaa ttcttacgtg
     4621 aaggtcgagc actttacgat aacttatacc cttctcgtat tgtgattggt gaacaatcag
     4681 aaagagcaca aaaatttgca gaccttttac aacaaggtgc attaaagaaa gatattgata
     4741 ttctattcac tgattccaca gaagcagaag caattaaatt atttgctaat acttatctag
     4801 cattacgtgt tgcttatttt aatgagctag atagctacgc tcaagcttac aacctaaatg
     4861 caaaacaaat tattgagggt gtctgcttag atccgcgtat tggtaatcac tataataacc
     4921 catcattcgg ttatggcggt tactgcttac caaaagacac taaacaacta ttagcaaact
     4981 acgactcggt tcctaataat attattagtg caatcgtaga agctaaccgc acacgtaaag
     5041 actttattgc agactctatt attgcaaaat caccaaaagt cgtcggtgtg tatcgtttaa
     5101 tcatgaagtc gggttctgat aatttccgct cttcttctat ccaaggcatt atgaaacgta
     5161 ttaaggccaa aggtattgaa gtagtgattt atgaacctga aatgcaagaa gaaaccttct
     5221 ttaattcaag attagttcgt gatttaaatg aatttaaatc aatctctgat gttattatta
     5281 ccaacagaat gtcatcagtg ctaaacgacg ttgaagaaaa agtttatact cgtgacctat
     5341 ttggtaacga ttaattaata aatacgggtt aattacgtaa tgaaatattt agtcactggt
     5401 gcagcaggat tcattggttt tcatctaata aaaaaactaa tccaacaagg tgaaactgtt
     5461 gttggtattg ataatctcaa tgattattat gatgtctcct taaaagagga gagacttaac
     5521 attctaaatc aattaaataa tttttcattt tcccttattg atctagctga tagggaaaaa
     5581 atagctagtc tatttgaaac tgagaaattt gatagagtga ttcatttagc tgcgcaagca
     5641 ggcgttagat attcactaat taacccgttt tcttatgctg atagcaattt aacaggcttt
     5701 ttgacaatct tagaaggttg tcgccataat aatgttaaac atcttgttta tgcttcatcc
     5761 agctcagttt atggtttgaa tgatgaatta ccattttcac ctcacgatca agctaatcac
     5821 cctgtttctt tatatgcagc aacgaaaaaa gctaatgaat taatggctca tagctattct
     5881 catttatatg gtataccaac aactggatta cgctttttta ccgtttatgg cccttggggt
     5941 cgtcctgata tggcattatt taaatttact aaagccatta tcaacgacga tcctattgat
     6001 atttataata atggtcaaat gagacgcgac tttacttatg ttgaagatat cgtggaaggc
     6061 gttgcccgca tagctgacgt aatacctgca ccacaacaag attggaaagt tagcacaggt
     6121 acaccagcta atagctctgc accttataaa gtctataata ttggtaatgg ttccccagtt
     6181 aatttaatgg attacattag tgcattagaa acccatttag gaaaaactgc tgataaaaat
     6241 atgttaccaa tgcaaccagg tgacgtttat accacatggg cagatactga ggatttattt
     6301 aaagcaacag gctacaaacc tcaaacaagc gtaaatgaag gtgtaaaaca gtttgttgat
     6361 tggtataaaa actattatca agttaaataa accatgctca atatcgtctt atttgaaccc
     6421 gaaattccac cgaatacggg taatattatc cgcctgtgtg ctaatacagg ctttcatctt
     6481 catttgatcc aacccttagg ttttacttgg gatgataaac gcctacgtcg tgcagggctt
     6541 gattatcgtg agtttgctga tattaaacag caccacgatt actatgcttt tttagaaagt
     6601 gaaggtttaa acccagataa tgcacaatca ccaacaggtg cacgtgtatt tgcgttaacg
     6661 acaaaaggta caccagcaca tagcaatgtg agctatcaag atggagatta tctccttttt
     6721 ggacctgaaa cccgtgggtt accgccttat gtattggata atatgccaac acagcagaaa
     6781 atcagagttc caatgcttgc tgatagtcgc agtatgaatc tatcaaacac cgttgccgtt
     6841 gtggtttttg aggcatggcg tcaactcggt tatcctggtg cattattaaa gtaattttta
     6901 tgaaaacatt ttttctaaaa aaactaagtt cacttatagt aaaaatttat gtcttcattt
     6961 tttataatga attaatttat aacaaaaata ttatatattt tggttttttt actattaaaa
     7021 atataaaaaa actatctatc aaagggaggg taaatataaa tgatcatgta tatattaatg
     7081 caaaaggagg cattactctt ggtgataaag taacactatc agcaggatca aaattaattt
     7141 caacaggatt attgattaat aataatggaa ttactactga acatatagat aaagaaatta
     7201 taatttcaga taatgtacaa ataggcgctg gtgcaatcgt acttgctgga gtatctatat
     7261 gtgaaaatgt tgttgtaggg gcaggctcta ttgtaactcg tgatattgaa aaatctggtg
     7321 tctatgtcgg aagcccagca aaatgtataa gactgtttaa ataatttaag aatgattatt
     7381 agaaatgttt tttatctaca gttaattaaa gcaatcgaat tactgatccc tttaattgca
     7441 attcctatag ttataaaata ccttggtata tctaactatg gcattatagc tttactccaa
     7501 gctatttcag catatattat tcttattggt gaatggggat ataatgctaa tgcaacaaga
     7561 gaattttcat ctgacatctc tgcttttgaa aaagcattat tatacataga gatacaaaaa
     7621 gtaaagattg tattctgcat attaatattt atatgcctaa ttttattaaa atttattatt
     7681 gaactaactt acttacaaat atccatcttg ttcgtatgga tttctacttc aatacttcaa
     7741 tgtaaatggt atttccaagc cacatataag ttaataaatt tagtcattat ctctttactt
     7801 tctaaaataa tatatattgt ttatgtcttt ttatttgtta aatataatac aaaaatagag
     7861 gaatatcttc tttttttgat actacctaat ataattttat ttatatactc taattattat
     7921 ataataaaaa aactatccat aaataaaata aaaaataaaa gatgctttag tctatcaaca
     7981 aaaagtaagt cattattgat agaaggttgg cacctaacat caatgaggct tattacaggt
     8041 gctataaatc ccatagcact aattatagca aaatcaatat atggcataga atttgttggt
     8101 tttattggtg tagcgcaacg cattgtatcg gcgtcagtat ctatattaac accaattatt
     8161 gatgttactt ttcctaaaat gaattatttt tatttgaata ataaaaataa attttataag
     8221 tttataaata ttatcttgtt gttttttata ttcattcaaa tttcaggtat ttttacttat
     8281 acaatattag accaatatat tttttatata ctaaatatta attactctaa aaaatcatca
     8341 ctaatactat ccctttatct acttattata ttctttagcc tatcaaactc ttttttagtt
     8401 catatgttag ttataatgaa aagatcaaaa tatataagtc cattttcttt attaagtgga
     8461 atattaacaa tatcatttat gctatcaatt gaaagattca atcattctca atataacttt
     8521 gtcttttcta ttttattagg ccaattattc ctatttattt ctgtattata tttaaaaggt
     8581 aaaaataata tttcatgaaa aatatattga ttttatcaaa ctccctttct aatggtggtg
     8641 gtgaaagaat agcttgcaac ctagctaatg ggctaaaaga aaataaatat aaagtatact
     8701 tttatataat agaaaataac atcagctata ctttgcatga agatattata ctaataaaaa
     8761 acccaataaa tattaatctc ccttttattg gaaaaataat aaataatttt tcagcaacta
     8821 ttcatttatt ttttattctt aaaaaagaaa aaataaatac tattattagc catttattta
     8881 gagcaaatta tattaatata tttctttcac tctttaaaaa acataaaagt atagttgtta
     8941 cacatggctc aattttaaaa tattctaata ataatttaaa atcaaaaata aatatattct
     9001 taataaaaaa tacatttaaa catgcgacta aaaaagtatt tcttactcaa aggatgttaa
     9061 atgattactt acctattatt ggagaaaaaa ataactttgt tatcccaaat tgctataacc
     9121 ttgataatat acttgaatta tctcaagcaa ttgtaaatga tagctccttt tttcctagtg
     9181 aatacttcgt ctttgtagga agatttcatc ctgttaaaaa tatttctttc ttattagata
     9241 cgtttaaaac aaccaattat aagctattat taattggaga tggggatcta tataaaaatt
     9301 ataaagaaaa atatcaaagc aataatataa aatttttagg aaataaagag aatccttatc
     9361 cttatataaa aaatgcaaaa gcaacaatac ttacttctat atcagaggga tttccaaatg
     9421 ttttaattga atctctctat cttggtgtac caataattag ttcagactgt agaactggtc
     9481 ctagagaaat attaaatatt aatggggaaa taaaaaaagg agatattcta tataataaac
     9541 tgggtgtgtt atataatatt aatgattacc aaggattatt aaattctcta atatacatat
     9601 caaaatcaga taaaaatcat gataaaaact taatgactta ctccatgaaa aaatacaaca
     9661 taccctccat tgttaaagaa tatattaata taatttaaat cttatgatct ataataaact
     9721 aatacctcta tggaataata aaggaaagta tcgtgaatta tactctttct ttcctattac
     9781 ctgtgtactt ataattccta ttttttatac ttttatcatt tttccttttt attatcaaga
     9841 aaaactatac caattattta ctatagaaaa ttcaatattt tggataagtg taagcttagt
     9901 gttattaatt acatctaaat taatttatag tagaaaaaga ataaagcaat attttttctc
     9961 taaaaatact gcattaatat ttgcttttat atatgttgga attgtatttt tttatattat
    10021 tctcaatttt ataataattg gattcaatga attttattca ggatgccatt attgtggtga
    10081 agtattaggt ggattcgagt tactgagaat aattatattt gatcttggca ccgtcactat
    10141 attgttgcta cccatctgct tgataatatt tagaaaaaat aagatttttc tatctataca
    10201 tattattact actttattaa ttctaactta ttattatatt ggttcatatc gtaatcaact
    10261 tttaccaata atatttctat tcttcctcat atttattagt aactatcgtt ctttttgtaa
    10321 gaaaatattt tttattctct ttttatttct atctcctatc gctgctattt caatggcttt
    10381 ttatcattat tttaaagata gtgaatcacc attaacactc tatgattact tctctatgaa
    10441 tgaattttat tctaattata acaactttat tatatatgag tcaggtttct ctttagattt
    10501 accgttttct ggttctagct atttagggcc atttcttcat aaattaaaca cattaattga
    10561 tacagggttt aaaacatcag cctctcaaat ggcacagtat atggatacag gtattggata
    10621 tggtttttct ccattactag aagccttact gaactttggt gatttattct taataggggc
    10681 aattattgta gcctttacta tatatatatc tattacactt atcatcttgt ctaaaaatga
    10741 tttaatatca actatatact tttctttatt tgtatttttt atctttaata tcaatcgagt
    10801 agactttact tctgctttta atatttactt ccataaactt ctactcatct ggttttttac
    10861 tttttttaca aatactcata tctctaatga aaaacgataa aatatctgtc ttactttcca
    10921 tatataatga aatagatgaa tgggtgatac tatctattaa ctcaataatc gatcaaaata
    10981 catatgataa gtttaaaatt caattagtca ttgttataga taaccctaat aaatgtgaac
    11041 attataaaaa tataattaat aatttagaaa agaaaaacat tgaaatagtg attatagaaa
    11101 atagtataaa tcttggctta gcaaattcat tgaacaaagc atttgaatat agtgatggtg
    11161 attttattgc tcgattagat gctgatgata ttgcttatcc agaacgatta tatgcgcaat
    11221 tgaaatatta taaactcaat tcttttgcat taatcggcac aggtataaat agaatcgatg
    11281 aacaaggtaa ctttatatct acatccttgt taccaaaaga tttcttaaaa ctaaaaagaa
    11341 cattaggtta taagtccatc tgttatcatc caacatggtt tatgtcaaga aatatttttg
    11401 aaaaaatgca aggatataga aaatacccta actcgcaaga ttttgatttt atttcaaggc
    11461 ttattgatga aagttatact atttcaaata ttgaagaacc attagtcatg tatcgaataa
    11521 atacacaatc tttatctttt aaaaactcat taaggcaaag aaaatgtcaa tatcatataa
    11581 taaaaatgaa taaaaataga aaaaaacatg gtatagataa ttataatgaa caaaagatgt
    11641 tagattatat caaaacaaat aaaatcattg atgcattaca ttttaaaagt cagttattat
    11701 tagtaaaagc gcttagaatg aataaattaa acccttgtaa gtatttactt ttattgtgtt
    11761 ccacattgct ttctccatac caaacaaagc atatattaag aatgagttat ttcaaaataa
    11821 aaaattactt tatataagta ttgtaactgc aattatagtt ttgaggcatg gcgtcaactc
    11881 ggttatcctg gtgcattgct aagagactaa aattaaaaag cgctgatcat aaataatgtc
    11941 tagcgctttt tttatttcaa acagctaaaa ttttaattta tgactctgat aattgtctga
    12001 tttcggcaac aacttttgta atataccgac acccatctcc cacatccttt aatactaatg
    12061 cgtgagcacc aattactgca ttatcaccaa tctctatctc accaccgata atcgaagaat
    12121 tacaaccaat ataaacatgg ttgccaactc gaatatctgc tttgccatcc ttttctttta
    12181 ctccaatggt cacaccttgc attaaggtta cgttctcacc taattgacag taattagtca
    12241 gtacaatccc gattggatgc ggtaacgaaa ggcctttacc tatgcgagtt cttaatgaga
    12301 tatcgttaca atatttacga ttgtttttag cacctatacg aatgcctaat tttttaagaa
    12361 aacgattttt agaaaaataa aagaattggg cgacacgcca ccaaaataga taacgtgaac
    12421 gttggcaaaa aaagacacgg tgaaaaagac gtatccaact aaaaggcttg ttatcgccaa
    12481 tatattcagc gtgtaaacaa gccttaagtt cagaaagtgt aacttctact aatgccatta
    12541 aatgccatca ccgcattcaa aacccggcac cacaccatta aagtgttgat ccatatcgag
    12601 tgaaggcttg tcactggtag gcttaccgac aatacgagcg ggaacgccag caacagtagt
    12661 gtgtgcagga acttcatgta aaacgactga tcccgcacca attttagcac ctcgaccaat
    12721 ctcgatatta ccaaggattt tagcacccgc accaatcatc acaccttcac ggactttggg
    12781 gtgacgatct ccccctgttt tgcctgtccc gcctaaagtg acggactgta ggattgatac
    12841 atcgttctca acaactgccg tttcaccaat cacaataccg gttgcatgat cgagcataat
    12901 accgtggcca atgcgcgcag cagggtgaat atcgacacca aaggtcacag aaatttgatt
    12961 ttgcagataa acggcaaggg ctttgcgatc ttggttccac aaccagtggg ctatacgata
    13021 agcttgtaag gcatgaaagc cttttaaata aagcagaggc gttgagtatt tatcgacagc
    13081 cggatcacgc aggcgaaccg ctgaaatatc ataagcggcg cattcaatca tcgaggcatc
    13141 gctacgatac gcttcttcaa caatttcacg aacagcaatc gcaggcatag tctgagtcgc
    13201 taatttattc gccagcatat aacttaaggc actacctaag ttttcatgct tgagtaatgt
    13261 cgcatggaag aaactcgcca aaataggctc acaatccgcc aaacatttag cttcactttt
    13321 aatatgatcc cagacttttt ttagttcttc tagcgacatg ctcactctca ttcttttaca
    13381 ttgaagccta agtaaaaact taggcttctt atgtttaaaa ccaaaactga tgccttaagc
    13441 aactatctaa aatagactta gaatagatta ctgtctatct catctttagt ggctctacct
    13501 aacaaagata atgccgcttc tttcgcgtct ttatgctgat ataaaatttg gtagatctgc
    13561 tcagtaatag gcatttcaac accaacttgt tctgctaatg cgcgaacttc cttggtattg
    13621 cgatagcctt caactacttg cccaattttc tcttgtgccg tatcaacatc aagcccttga
    13681 cctaacatca tgccaaaccg acgattacga gattggttat cggtacaggt taaaactaaa
    13741 tcccctaacc ccgccattcc cataaacgtt gcggcatctg cacctaatgc tttacctaaa
    13801 cgactcattt cagcaaggcc gcgagtaatt agtgctgtac gtgcattcgc accaaacccc
    13861 ataccatcag acatacctgc gccaatagca atcacgtttt ttaccgcgcc acctaattgc
    13921 acgccaataa agtcaggatt tttatacaca cggaaacttt tgccacaatg gaacaactgt
    13981 tgtaactctt cagaaaaggt attgtccgtt gatgcaacag aaatcgcagt aggcagacct
    14041 gctgcaagct ctttggcaaa agtagggcca gataaaactg caagcgggat ttcattacct
    14101 aatacttcac gcgctacatc ttgcaataag cgaccagtat gagcctcaag tccttttgtc
    14161 gcccaaacaa cacgagcatc ctgacgtaaa aagggtttga tctgctgtaa tacgttacca
    14221 aaaacatggc ttgggatcac aaccagaata ttacggctgg cttcaatggc tttttgcaaa
    14281 gaagcttcca tgtataaact atcaggaaag gaaacatcgg gcagaaatgc ctgattacag
    14341 cgtgcttgtt ctaacgccgc aacatgctca ggatcatgac cccaaagcac aacatcatga
    14401 ccattgcgcg ctaaggtaat cgctaaagcg gtgccgtatg aaccggcacc gataactgtc
    14461 atagaagcgt tcattaagct tcttcagccc cttcattagc tgcatcagtt tgttgctgtt
    14521 gtaaatagtt cataaacaac gcatcaaagt taac
//
