LOCUS       KY710701               12619 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 47/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710701
VERSION     KY710701.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 12619)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12619)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12619
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 47/57"
                     /serotype="O24"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..12619
                     /note="O antigen gene cluster"
     gene            2..673
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             1095..2303
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippas"
                     /protein_id="AXY99565.1"
                     /translation="MILSKILLAVKKNKHIMYLLITTFLSMAISMLNTIIIARFLGAN
                     GRGTIQLILTAQGLLATACSFGLGVSYIYNLKKKCIDIGLIDIILKVILFSIISSIIF
                     IYTQDINDFLFPIIFIVLTTSISFTLLDIMKVEKDLLGYRYATLIPILLNFTLNIFLI
                     FLDKFNISSAINTLLFSQLIQTIYIVYYCNKKIQIVKFKFYKFIKIFTYKETKYYVFI
                     LGFTIVVTSISNHIDKLVVYNILGLYELGKLSIIISLCAILNKIFDILSTSYFSHRLQ
                     KNNKPKNIVKIFLLIILLYFFISPLIYLIGNKFIPVIFGSEFIDLGDILVIMLLNSIV
                     TGFNSIIAQEFNIQGKPLFTLIRVSLSILLFIFLAYIFADFMLIGITISILLSSIFRL
                     ALSLTFLRKL"
     CDS             2300..3250
                     /note="orf03"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf03"
                     /product="polysaccharide pyruvyl transferase"
                     /protein_id="AXY99566.1"
                     /translation="MINIKPNKYQNTSIKNYLTEIHNDNGKFTSAIYLDIPCYHNLGD
                     MLIYLGAKKMLHDLNVTVRYTESALSHNLKKIKRILKQHPDTVILMQGGGNFGDLYSE
                     AQTYRLNIIKKFKNEKIIILPQTIWYDNYNNYLNDKEIIKKAKNLTIYTRDERSFNYI
                     SNITSIKINMMPDTAHWLWDDGINILNKKINHKGTKKILIHRTDDELSTESLKDDNYH
                     KIFDWPNLESSKDKLFEKIIRFQQRKLPFLNNLFIKKWEKHMYSFCSKGVSELNEAND
                     ITTSRLHGFILSYIMNKNVSVLDNRYGKNYSYIKKWINND"
     CDS             3243..4091
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99567.1"
                     /translation="MTNLPLVSVIIPVYNAEKYLEKAINSIVNQSYRNLEIIVINDGS
                     VDHSHLIVTSFTDSRIKYIKQDNQGLSKTLNSAIRIASGKYICRMDADDISDLDRIKI
                     QIEILEKNKNIILVGSNIMYIDENDKIKGYSYLPSKSDTIKNKLRIDNCIFHPSVIFR
                     KSIFEQCGGYNESVNIFFEDYLLWLSMLPHGDFYICPKFLLKYREHDKSITNRTPPIV
                     KNLMKKYSIHGKLTEQEIRLLHEKRNILNNKKNKLSTIQKVKKFIFRSKLLSILTIKI
                     RNIIHI"
     CDS             4189..4998
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99568.1"
                     /translation="MLDVTVIFSIYEKTNLSKFKLALNSILNQTLLPKYIIIVCDGCS
                     FDMFESCLDEHLSSLVKINIYEYKENLGPGYARDFAIKRANTKYIAIMDSDDISALDR
                     LEIQYNFMEKNNNISVVGGYISEEKDGNFISIRKVPLHHKDISKTIKYKSPINNVTAF
                     MRRKDYIYVGGYPSIRSSEDYCLWSRFLIHGKKMANIENILVYVEFDEDTLIRRSGLL
                     HFKNDLFTQKTLYKSGIINIFTFNKNIFKYFIFRLMNIKIKKFIYTYVLRK"
     CDS             5003..5707
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99569.1"
                     /translation="MLKVIVSTMDGNFKPEYFPEGEYEIIIVNQGKKCNLQEENEYIK
                     VINSNTLGLSNSRNVGLTQCNEDDIIIFTDNDVSFYKDFDKLIYNIFLLSNKDIVTFK
                     VKNIHEKPFKNNYINKKFKHNLISIMKVSSIEIALKYNKKIKPFDNSFGLGTSIPLGE
                     ENIFLSDNIKANQQVYFEPVYLCKHMDDNHSGANFNKKTFYMRWKIFKRIYGKLVGSI
                     IFLIFNIKNLRKYFIK"
     CDS             5713..6888
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99570.1"
                     /translation="MKKLLLFSVFISFFFQSFYIDIGYAIKAFMVLIPLLYFFTSFGK
                     FNIKFYNHEVLLFFTIIIFSSSLIYSYDYLSSFKMIVGLLILILLFNLLLTSITSFNP
                     SVFFKYIYLSGLFFNIISLLLYFYGIIMVKGLFAFETQGVHYGLLVDRGIPRLIGTLK
                     DPNFFIAFNSIFLFLSYYNFKESKTAKLLFILSSVTCLSTFSIGGFIAIICGFITMSL
                     QNKKGIFRLLSLILLILCIYFISIWLYPEINKFIMSRLSGASDGSGRFNDWKNALITF
                     LNYPFGIGIYSFLAYNQEILGGIHYVHNTYLEVLVEGGIHTFSFYILFIIVLLLTVYK
                     LGKKNEYFEFLLPSTITLLMSFFSLSGFASEFWLFYLAIVHFCCNKASNDNKLSQKN"
     CDS             6937..8103
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99571.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNQKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKGIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEDTFFNSKLIRDLDAFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     CDS             8115..8618
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXY99572.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     NGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(8758..9735)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXY99573.1"
                     /translation="MKNNMPKITVLMSVYNDEKYLQESIDSILNQTFKEFEFLIINDG
                     STDNSLSILKENAKKDPRIKLIINEQNIGLIASLNKGLQLASCDLVARMDSDDIAVST
                     RLEAQYQFMLQHPEVSVCGGAFIYQEEPNRINTYPETHSDIVISSLYDCSLAHPTIIY
                     RKQDILSVGGYDKADLYAEDYGLWVNLIIAGKFKLHNLPVPLLYYRTHPNVERSQYKA
                     IQKDTAERVGRKLLMYFGINVNKEDYSLLLDNDKKTVEQVVRCKRLLEKINQQFNAKE
                     YRKELIQKNEFKKRLLERKVLVTDFSSFLIKVSARWYFLIRKITKKAKK"
     CDS             complement(9745..10302)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemH"
                     /product="wemH"
                     /protein_id="AXY99574.1"
                     /translation="MHAQAKVTLAELKDCLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGDHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGEGCRYINKMNFEINKYC"
     CDS             complement(10305..11138)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99575.1"
                     /translation="MRVSMSLEELTRVWEHIKSEARALADCEPMLASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(11235..12251)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemH_2"
                     /product="wemH"
                     /protein_id="AXY99576.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSLL"
     gene            complement(12251..12445)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     4315 a   2038 c   1955 g   4311 t
ORIGIN
        1 gcgattgatt tctgatatct cccgtgaatt gcgcacccca ctaactcgtt tacagctggc
       61 cagtgcatta ctgcgtcgtc gtagcggtga aagtaaagag ctagagcgta ttgaaacaga
      121 aacacagcgg ctagatggca tgatcaatga cttattagtg ctttctcgca accagtataa
      181 aaatgagtta ttacgcgaaa ccgtaaaagc gaatgagctt tggaacgata ttttagataa
      241 tgcgaaattt gaggctgaac agagcaataa aaccctaaca gtaaccgccc cgccaggccc
      301 atggacgatt tattgcaatc cttattcatt agcgagtgcc tttgaaaata tcgttcgcaa
      361 tgccttgcgt tattcacatt ctcgtattga agtggctttt accgaacaaa atcagggtat
      421 cactattatt gttgatgatg atggcccagg ggttagccca gaagatcgcg agcatatttt
      481 ccgacctttt tatcgtacgg acgaagcacg tgatagagaa tccggcggta caggactagg
      541 actggcaatt gttgaaaccg caattagtca acatcgaggc catgtaaaag ccgatgatag
      601 cccattaggg ggattaagag tggagatttg gttgccgaga gggagtgtgg taaataaatc
      661 acaacacgct taattcagta ataaagttac tatattaaaa tattctgcca tctattgaag
      721 aataggtggc atattctttt cattcccatc tttatagata tttcagaatg atcttgctaa
      781 ttcaatcgat attccgatac ttatatatac ctaatcaatg ttagaatagc catcaattgc
      841 ttgacccgct ggtttgaaga gtctaatctc aaagcggttt tgcatactgt taaaatatgc
      901 ttatataaca aaactcttat attacagata gataagagat tttcgagaaa tttttggcgc
      961 tctgggggcc gtgaacccag tgcggtagct ttaaattttg aattatcttc aagtatagta
     1021 acaatatata aaaaattaca atctcgacta ccaattttag taataaattt cccttttata
     1081 gataaaactt agagatgatt ttgtcaaaaa tattattagc agtaaaaaaa aataaacata
     1141 ttatgtattt attaataact acatttctct ctatggctat atctatgctt aatactataa
     1201 ttatagctag gtttctagga gctaatggca gaggtactat ccaacttatt ttaacagcac
     1261 aaggactatt agcaacagct tgtagctttg gcttaggagt aagttatata tataatttaa
     1321 agaaaaaatg tattgatatt ggattaatag atattatttt aaaagtcatt ttattttcta
     1381 taatatcctc tataattttc atatatactc aagatattaa tgatttttta tttcccataa
     1441 tatttattgt tcttacaact tcaatatcat ttactctgct agatataatg aaagttgaaa
     1501 aagaccttct tggttatcgt tatgcaacgt taatccctat acttttaaat tttacattaa
     1561 atattttttt aatcttttta gataagttca acatatcatc cgcaataaat actttgctat
     1621 tttcccaatt aatccaaaca atatatattg tatattattg taataaaaaa atacaaatag
     1681 ttaaatttaa attttataag tttattaaaa tttttactta taaagaaact aagtactatg
     1741 tttttatatt aggatttact atagtagtta catctatttc taatcatatt gataaactag
     1801 ttgtttataa tatattaggt ttatatgaac taggtaaact atctataata attagtcttt
     1861 gtgcaatcct taacaaaata tttgatattt tgagcacgtc ttacttttct catagattac
     1921 aaaaaaacaa taagcctaaa aatatagtta agattttttt attaattata ttattatatt
     1981 tttttatatc tccattaata tacctaattg gcaataaatt catacccgtt atattcggaa
     2041 gtgagtttat tgatttaggt gatattttag ttatcatgct tttaaattca attgttactg
     2101 ggttcaattc aattatagct caagaattta atattcaagg aaaaccgtta tttacattaa
     2161 ttagagtaag cctttcaata ttattattta tttttttagc atatatcttt gcagatttta
     2221 tgctaattgg aataactata tcaatattac tttcatctat atttaggtta gctttatcac
     2281 ttacattttt aagaaaatta tgatcaacat taaaccaaat aagtatcaaa atactagtat
     2341 taaaaattat cttactgaaa ttcataatga taatggaaag tttacatcag caatatatct
     2401 agatatccct tgttatcata acttaggtga tatgctaatt tatcttggtg caaaaaagat
     2461 gctccatgat ctaaatgtaa ctgtacgtta tacagaatca gcattatcac ataatctaaa
     2521 aaaaataaaa cgtattttaa aacaacatcc tgatactgta attttaatgc aaggtggcgg
     2581 taattttggc gatctttatt ccgaagctca gacctatcgt ttaaatataa taaaaaagtt
     2641 taaaaatgaa aaaataatta tattacctca aactatttgg tacgataatt ataataatta
     2701 tctaaatgat aaagagataa taaaaaaagc taaaaatcta actatatata caagagatga
     2761 gagaagtttt aattatatat caaatataac atcaataaag attaatatga tgccagatac
     2821 agctcattgg ttatgggatg atggaattaa tatcctaaat aaaaaaataa atcacaaagg
     2881 aacaaaaaag atactcattc atcgaactga tgatgaatta tcaacagaat cattaaaaga
     2941 cgataattat cataagattt ttgattggcc aaatttggaa tcatcaaaag ataagttgtt
     3001 tgaaaaaatt atacgttttc aacaaagaaa actcccattt ttaaataatt tatttataaa
     3061 aaaatgggaa aaacatatgt attcattttg tagcaaaggt gtctcagagt taaatgaggc
     3121 aaatgatatt acaacaagta gactacatgg ttttatctta tcatatatta tgaataaaaa
     3181 tgttagtgta ttagataatc gctatggcaa aaattattca tatataaaaa aatggataaa
     3241 taatgactaa tttaccttta gtttccgtta taatacctgt atataatgca gaaaaatatc
     3301 tagaaaaagc gataaatagt attgttaacc aatcgtatag aaatctagaa ataatagtta
     3361 ttaatgatgg ttccgtagat cattctcacc ttattgtaac ctcttttact gatagtagaa
     3421 ttaagtatat aaaacaagat aatcaagggt tatcaaaaac attaaattct gcaataagaa
     3481 tagcttctgg aaaatatatt tgtagaatgg atgctgatga tattagtgat ctagatagaa
     3541 taaaaattca aatagaaata cttgagaaaa ataaaaacat tatattagtc ggaagtaata
     3601 taatgtatat tgatgagaat gataaaatca aaggatatag ctatttacca agtaagtcag
     3661 acactattaa aaataaatta agaatagata attgtatttt ccatccttca gtaatattta
     3721 gaaaaagtat ttttgaacaa tgtggtggat ataatgaatc agttaacata ttttttgaag
     3781 actatctttt gtggttatca atgttaccac atggagattt ttatatttgt cctaaattcc
     3841 tattaaagta tagagaacat gataaatcta taacaaatag aactccacct attgtaaaaa
     3901 acttgatgaa aaaatactct attcatggaa aactaacaga gcaagaaata aggttattac
     3961 atgaaaaaag aaatatttta aataataaaa aaaataagtt atcaacaatt caaaaagtaa
     4021 aaaaatttat atttagaagt aagttattat caatcttaac aattaaaatt agaaatatta
     4081 tacatattta ataaaaaaca agatgatttt tataaattta ttaagactag ttttaatacc
     4141 accccattta ctatatgaac attaacaaaa caacaattca gagaaattat gctagatgta
     4201 actgttatat tttctatata tgaaaaaaca aatttatcta aatttaaatt agcattaaat
     4261 agcatcctta accaaacatt attacctaag tatataatta tagtatgcga tggatgttct
     4321 ttcgatatgt ttgaatcatg tcttgatgag catttatcat ctttagtaaa gattaatata
     4381 tatgaatata aagaaaattt agggcctggc tatgcaagag attttgcaat taaaagagca
     4441 aatactaaat atattgcaat aatggattca gatgacatct cagcattaga tagattggaa
     4501 atacaatata attttatgga aaaaaataat aatatatctg ttgttggggg atatattagt
     4561 gaagaaaaag atggtaattt tatatccatt cgtaaagttc cactacatca taaagatatt
     4621 tcaaaaacga taaagtacaa atcaccaata aataatgtta ctgcgttcat gcgaagaaaa
     4681 gattatattt atgttggtgg gtatccttct atacgcagct ccgaagatta ttgtttatgg
     4741 tctcgcttcc ttatacatgg aaaaaaaatg gctaacatag aaaatatatt ggtatatgtt
     4801 gaatttgatg aagatacatt aataagacga agtggattat tacattttaa aaatgattta
     4861 tttacacaga aaactctcta taaatctggc atcattaaca tatttacatt taataaaaat
     4921 atttttaaat attttatatt tagattaatg aatataaaaa taaaaaaatt tatctataca
     4981 tatgttttaa ggaaataaat ttatgttaaa agttatagtt tcaactatgg atggaaattt
     5041 taaacctgaa tattttcctg aaggtgaata tgaaattata attgtaaatc aagggaaaaa
     5101 atgtaactta caagaagaaa atgagtacat aaaagtgata aatagtaaca ctttaggttt
     5161 atctaatagc cgaaacgtag gattgacgca atgtaatgaa gatgacatta ttatctttac
     5221 tgacaatgat gtatcttttt ataaagattt cgataagtta atatataata tcttcttgtt
     5281 aagtaataag gacatcgtta cttttaaagt aaaaaatatc catgaaaaac cattcaaaaa
     5341 taattatata aataaaaaat tcaaacataa tttaatttca ataatgaagg tctcatcaat
     5401 tgaaatagcc ttaaaatata ataaaaaaat aaaaccattt gataacagtt ttggattagg
     5461 aacaagtata cctttagggg aagaaaatat atttctttct gataatataa aagctaatca
     5521 acaggtttat ttcgaaccag tttatctatg taaacacatg gatgataatc attcaggtgc
     5581 taattttaat aaaaaaactt tttatatgcg ttggaaaatt tttaaacgta tatatggtaa
     5641 actagttggt agcattatat ttcttatatt taacattaaa aatctaagaa aatattttat
     5701 aaagtgaatt ttatgaaaaa attattactt ttcagcgtat ttatatcttt tttctttcaa
     5761 tcattctata tagatatcgg ctatgctatt aaagctttta tggttttaat tccattactt
     5821 tatttcttta cttcatttgg aaaatttaat ataaaatttt ataatcatga agttttgtta
     5881 ttttttacaa taataatttt ttctagttcc ttaatttatt cttatgacta tttatcatcg
     5941 tttaaaatga tagtcggctt actaatttta atcttactat ttaacttatt actaacctcc
     6001 ataacatctt ttaacccatc agtttttttt aaatatattt atttatctgg tttatttttt
     6061 aatataattt ctttactgtt atatttttat ggaattatta tggttaaagg actttttgca
     6121 tttgaaacgc aaggagtgca ttatggtctt ttggttgata gagggattcc tagattaatt
     6181 ggaacattaa aggatccaaa ctttttcata gcatttaata gtattttcct atttctttca
     6241 tattataatt ttaaagaaag taaaacagcg aagttacttt ttattttgtc cagtgtcact
     6301 tgtttatcta ctttttctat tggtggtttc attgctataa tttgtggttt tattacaatg
     6361 tctttacaaa ataaaaaggg tatatttaga cttctttctt taattttact tattctctgt
     6421 atatatttta ttagtatctg gttatatcct gaaattaata aatttataat gtctagatta
     6481 tcaggagcat cagatggaag tggtcgtttt aatgattgga aaaatgcatt aataactttc
     6541 ttaaattatc catttggtat aggaatttac tcgtttctcg cttataacca agaaattctt
     6601 ggaggtattc attatgttca taatacatac ttagaggtat tagtcgaagg aggcattcat
     6661 acattttctt tctatatatt atttataatt gtattattgc taactgttta taaattaggg
     6721 aaaaaaaatg aatattttga atttttacta cctagtacaa ttacattatt gatgtcattt
     6781 ttttctttat ctggatttgc cagcgaattt tggttatttt atttagctat agttcacttt
     6841 tgttgtaata aagcgtcgaa tgataacaag ttaagtcaaa agaattaaat aaccagaact
     6901 acttatttta ttaagataat accttgagta aaagttatga aaatcaccat ctccggtacc
     6961 ggctatgtcg gtctatctaa cgggatactc ctttcccagc acaacaaagt tgttgcatta
     7021 gatattaata aagaaaaagt cgaactatta aaccaaaaaa tctctcctat ctctgataaa
     7081 gaaattgaag agtttttagc tactaaagag ctcgatttta cggcaacttt agataaagaa
     7141 ttcgcttata aagatgctga atttgttatc attgccactc ctaccgatta tgatcctaaa
     7201 actaactact ttaacacatc ttctgtagag tcggttattc gtgatgtttt agaatataat
     7261 ccaaacacca ccatgattat aaaatcaacc attccggttg gttttactaa acagatgaga
     7321 gagaaatata atactgataa tattatcttc tctcctgagt tcttacgtga aggtcgcgcc
     7381 ctttatgata atctctatcc atctcgtatt gtgattggtg agcagtctgc aagagcagag
     7441 aaatttgctg agcttttaca acaaggggca ttaaaaaaag gtattgatgt cctttttact
     7501 gattccacag aagcagaagc aatcaaacta tttgctaata cttatctcgc actgcgtgtg
     7561 gcttatttta atgaactcga tagctacgcc caagcttacg gcttaaacgc aagacaaatt
     7621 attgaaggtg tctgtttaga tccgcgcatt ggtaatcact ataacaaccc atcatttggt
     7681 tatggtggct actgcttacc aaaagatact aaacaactgt tagccaacta cgaatcagta
     7741 cctaataata tcattggtgc cattgttgaa gctaaccgca ctcgtaaaga ctttattgct
     7801 gattctatta tcgctaaatc accgaaaatt gttggtgttt atcgtttaat tatgaaatca
     7861 ggctctgata atttccgctc atcttcaatc caaggcatta tgaagcgtat taaagcgaaa
     7921 ggcattgaag tggtaattta tgagccagaa atgaaagaag atactttctt taactcaaaa
     7981 ctcattcgtg atttagatgc gtttaaagca atgtctgatg tcattattac caatagaatg
     8041 gcttctgcac taaacgatgt tgaagaaaaa gtttatactc gtgatctctt tggtaacgat
     8101 taaaactaat ttatatgctc aatatcgtct tatttgaacc cgaaattcca ccgaatacgg
     8161 gtaatattat ccgcctgtgt gctaacacag gctttcatct tcatttgatc caaccattgg
     8221 gttttacttg ggatgataaa cggctacgcc gtgcagggct tgattatcgt gagtttgctg
     8281 atatcaaaca gcatcacgat tattatgctt ttttagaaag tgaaggatta agccctgata
     8341 atgcacaatc cccgacaggt gcacggttgt ttgcattaac cactaaaggt actcccgccc
     8401 atagtaatgt aagttatcaa aatggggatt atctgctatt tggccctgag acacgaggtt
     8461 taccacctta tgtgttggat aatatgccaa cacaacaaaa aatccgtgta ccaatgttag
     8521 cagatagccg cagtatgaat ctttctaata cggttgccgt ggttgttttt gaggcatggc
     8581 gtcaattagg ctatccgggt gcgttattaa gagattaatt tatcttttta atttataacc
     8641 ataaaaaatg acctctaaac ttcattgtct cgaggtcatt tttatgtata tgctttgtgc
     8701 tagttttagc ttttcttatt ttatatcttc tttgtatctt ttttatatct ttcggtttta
     8761 tttcttagct tttttagtta tttttcttat caagaaatac catcttgcac ttacttttat
     8821 taaaaatgat gagaaatcag ttactaatac ctttctttct aataatcttt tcttaaattc
     8881 atttttttgt attaactctt ttcgatactc tttagcatta aattgttggt taatcttttc
     8941 aagtaacctt ttacagcgta ctacttgctc tactgttttt ttatcattat ccaataataa
     9001 tgaataatcc tctttattca cgttaatacc aaaatacatt aataattttc gtcctactct
     9061 ttccgcggta tctttttgta tcgctttata ttgcgaacgt tcgacatttg gatgagtacg
     9121 ataataaaga agtggtacag gaagattatg gagcttaaat tttccagcaa taattaaatt
     9181 cacccataaa ccataatctt ctgcatagag gtctgcttta tcatatccac caacagataa
     9241 tatatcttgc ttacgataga taatggttgg atgcgctaag gagcaatcat aaagactaga
     9301 aatcacaata tcactatgtg tttctggata agtattaatt ctatttggct cttcttggta
     9361 gataaaagca ccaccacaaa cactcacttc aggatgttgt aacataaatt gatattgcgc
     9421 ttctagtctg gttgaaacgg caatatcatc tgagtccatt ctagcgacta aatcacatga
     9481 agctaattgt aatcctttgt ttaaagaagc gatcaatccg atattttgtt cattaataat
     9541 cagttttatt ctgggatctt ttttggcatt ttcttttaaa atagataatg agttatccgt
     9601 tgagccatca ttaatgataa gaaactcaaa ttctttaaat gtttgattta atatagaatc
     9661 aatagactcc tgaaggtatt tttcatcatt gtataccgac attaatacgg taattttggg
     9721 catattgttt ttcattatct attttcagca atatttatta atttcaaaat tcatcttatt
     9781 gatataccga cacccttctc ccacatcctt taatactaat gcatgggcgc caataatcgc
     9841 attatcacca atattaatct caccaccaat aatggatgaa ttacagccaa tataaacatg
     9901 atctcctact tggatattgg cttttccccc tttctctttt acgccaatag tcaccccttg
     9961 cataatagtg acatattccc ccagttggca gtaattagtc agtacaatgc caatcgggtg
    10021 tggtaaagaa aggcctttac caatacgagt ttttaacgag atatcattac agtatttacg
    10081 gttattgcta gcaccgatcc gaatacctaa tttatgtaaa aagcgatggg gagagaaata
    10141 aaaatactgt gctacccgcc accaaaatag gtaacgggaa cgctgacaga aaaatactcg
    10201 atgaaatagc cttaaccaac taaagggttt tttatcacca atatattcag cctgtaagca
    10261 atcttttagt tcagcaaggg taaccttagc ttgagcatgc atagttaaat accatcacca
    10321 cactcaaagc cggggacaac accattaaag tgttgatcca tatcgaatga tggtttttca
    10381 ctatcaggac gaccgacaat gcgggcaggc accccggcca ccgtagtatg agctggtact
    10441 tcatgtaata ctaccgagcc agcaccaatt ttggcaccac gaccaatctc gatattacca
    10501 agaattttag ctcctgcgcc aatcattacc ccttcacgca ctttagggtg acgatcgccc
    10561 cctgttttac ctgttccccc taaggtcaca gattgtaaaa tggagacatc attttcaact
    10621 accgccgttt caccaattac aatacctgtc gcatggtcga gcataatacc gtgtccaata
    10681 cgtgccgctg ggtggatatc caccccaaag gtgactgata tttggttttg tagataaata
    10741 gctagcgctt ggcgattttg ttgccatagc caatgggcaa tacggtaggc ttgtagtgca
    10801 tggaaccctt ttaaatagag cagtggggtt gaatatttat ctaccgcggg atcacgtagg
    10861 cgtaccgcag agagatcaca agcggccgat tcaatcattg agctatcgtg acgataagcc
    10921 tcttcaacga tttcgcgtac tgcaatggct ggcatagtct gagttgccag cttattggcc
    10981 agcatatagc ttaatgcact acctaaattt tcgtgtttga gaagtgtcgc atgaaagaaa
    11041 ctggctaaca ttggctcaca atctgccaat gcacgcgctt cgcttttaat atgctcccag
    11101 actcttgtta gttcttctag cgacatactc actctcattt tttacaccaa agcctaagta
    11161 aatacttagg ctttgaattt tcaaacaatt ggatagttaa aacagatacc ctaagaatat
    11221 tatttaaaat aaacttacaa caggctgctg tctatctcat ctttggttgc tcgccctaat
    11281 aaagccaatg cagcctcttt tacatcttta tgttgatata aaatttggta aatctgttcg
    11341 gtgattggca tttctacacc cacttgttcg gctaatgcgc gaacttcttt ggtgttacga
    11401 taaccttcga ctacctggcc aattttctct tgggctgtat caacatctaa accttgacct
    11461 agcatcatac caaagcgacg attgcgtgat tggttgtcag tacaggttaa aactaaatca
    11521 cccaaaccag ccatgcccat aaaagttgcc gcatctgcac ctaaggcttt acctaagcgg
    11581 ctcatttcgg ctagacctcg ggtaatcagt gcggtacgcg cattagcgcc aaatcccata
    11641 ccatcagaca tacccgcacc aatagcaatc acgtttttaa cagcgccccc cagttgcaca
    11701 ccgataaaat cagggttttt atagactcgg aagcttttac cacaatgaaa tagctgttgt
    11761 aactgctcta aaaagagatt gtctgtcgag gccactgcaa tcgcggtggg caaacccgcg
    11821 gcaagctctt tagcaaaagt agggccagat aatactgcga gcgggatttc attacctaat
    11881 acttcacggg caacatcttg taataagcga ccagtatgtg cttcaagacc ttttgtcgcc
    11941 caaacaacac gtgcatcctg acgtaaaaag ggtttgattt gttgcagtac ttcaccaaac
    12001 acatggcttg ggatcaccac aagaatatta cggctcgctt caatcgcttt ttgcaaagaa
    12061 gcttccatat ataaactatc aggaaaggaa acatcgggca gaaaggcttg attacagcgc
    12121 gcttgttcta atgccgcaac gtgcttggga tcatgccccc acagcacaac atcgtgccca
    12181 ttacgcgcta aggtaatcgc taaagcggtg ccgtatgaac cggcaccgat aactgtcata
    12241 gaagcgttca ttaagcttct tcaactcctg agtttgctgc atcctgttgc tgttgttgct
    12301 gtaagtagtt cataaacaac gcatcaaagt taacaggtgc taagtttaat tgtgggaaag
    12361 taccacgacc tactaagttg gtgatacatt cacgtgcata agggaatagt acgttcaggc
    12421 aatatgcacc taagcaatgt gtcaattttt ttatttctct aaaaatcgtt ttctcaaaaa
    12481 attaggcatt tacattggtg ctaaaaacaa tcgtaaatat tgcaatgata tctcattaag
    12541 aacgcgcata ggaaaaggcc tttcattacc acatcctatc ggtattgtgc tgactaatta
    12601 ctgtcaatta ggtgagaat
//
