LOCUS       KY710702               17335 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus vulgaris strain PrK 48/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710702
VERSION     KY710702.1
KEYWORDS    .
SOURCE      Proteus vulgaris
  ORGANISM  Proteus vulgaris
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 17335)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17335)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17335
                     /organism="Proteus vulgaris"
                     /mol_type="genomic DNA"
                     /strain="PrK 48/57"
                     /serotype="O25"
                     /db_xref="taxon:585"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..17335
                     /note="O antigen gene cluster"
     gene            2..628
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             1039..1920
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlD"
                     /product="rmlD"
                     /protein_id="AXY99577.1"
                     /translation="MEKIRQVGWELQRSLVPLGTVIALDSKSTEYNADFLFPSKLEKT
                     ILSIKPDVIVNAAAYTNVDKAETDDINAFKINAESVEIIAKTAEKIGALLIHYSTDYV
                     FDGTNDTPWLETDTPAPLNIYGKSKLAGEQAIISNCKKYLIFRTSWVYASRGNNFAKT
                     MLKLGRKYDSLSVINDQYGAPTSAELLADCTAHAIKSTLDNGKPLYGLYHLTAKGTTT
                     WFEYASLIFKYAREYSLPLVVKNLTPIPATEYPLPASRPYNSRLNIQKFEKNFRLELP
                     NWKFGIYRMLNEISDNK"
     CDS             1946..2824
                     /note="glucose-1-phosphate-thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlA"
                     /product="rmlA"
                     /protein_id="AXY99578.1"
                     /translation="MKKYKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGNGNQWGINLQYKIQPSPDGLAQAFILGEDFIGS
                     DNCALVLGDNIFYGHDLPKQLEQAIQKEHGATVFAYHVNDPERYGVVEFDKNKIAISL
                     EEKPLKPKSNYAVTGLYFYDNDVINIAKNLQPSPRGELEITDVNKVYLEKHKLSVSIM
                     GRGYAWLDTGTHKSLIDASNFIQTIEDRQGLKVSCPEEIAYRKGFINKEQVRILAEPL
                     SKNDYGRYLLQIIQSD"
     CDS             2827..3372
                     /note="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlC"
                     /product="rmlC"
                     /protein_id="AXY99579.1"
                     /translation="MNIIKTEIPDVLILEPKVFGDHRGFFYESFNQKIFEEAVGYPVN
                     FVQDNHSKSSKGVLRGLHYQKAPYAQGKLVRTVLGEVFDVAVDIRPESSSYGKWVGVT
                     LSAKNKRQLWIPEGFAHGFLVLSDEVEFLYKTTNFYHQQSESHIVWNDPDLNINWPIK
                     DSPILSEKDKTGLYFNKLVGM"
     CDS             3374..4462
                     /note="orf05"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf05"
                     /product="hypothetical protein"
                     /protein_id="AXY99580.1"
                     /translation="MKEKILLQLDHWLDKECILYYIDYDLKNNSYSKTGSIVKRFVLP
                     KNHEQLINVICFSKSIDLDIKIIGETTNILFNDTVNYGVFISTKLVNNVQFKDSTVIV
                     ESGKSLPDFVRDVSMRGYGGYEGLEGIPGSIGGGIVMNAGAYGYEISDNIISVTYLNE
                     NGEVITKDKSSLNFEKRNSIFKYKDLIILYAEFQILRSPLEDIKNKIETFHIARHIYQ
                     EWTMPNLGSIFTAPRSLYDHFKFSSFKNELSYKLTKKIFYNKIMRFFNRKKPSNERLN
                     NIIKIDKNLDLLTVNCSHKNINTFCNQNNSTREIIDYIYSIYNSIDKKNVFLENELVE
                     KSIYEIISENDYKDITFKYKKILENEKI"
     CDS             4449..5960
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99581.1"
                     /translation="MKRFSNVLKNSVLLYFRMVVGLFVSLYISRISLDILGVDTFGLY
                     TIIASFVIVGGVLVNVATVSMQRHLSEKIGENLSRTLSTESKNVLDACVIVNTMLGIF
                     SILLIILFGTVYINLYLNQDIVNISIIWYVFYVSIATFLISFTTSPYLTLLISFEDTK
                     YYALIMILEIISKLISILCLYLTGSKSILDYTIAIFISILITRLILFLIVKNRYKNIP
                     LTLTYDWNIIKPILSFTGWNLWGGIASVMSLQGVTFLINIFFGLSVNTARAISLQIYS
                     AVTQLVNSIQIAIAPQLVKSNGKENNYFSQLFILSGKITIYLAFLISLILYENTESLL
                     DLWLDNYPPVTIIFLKFTYIEIILTSLGYPVLSAVQANGNIKKYQLVVGGLMILNIPF
                     IFISISLYETYISIYIVSIIISSISLLYRLIYAKKIMGSIVNEYLFSVVIKGILVLLL
                     SLILTNLIHCNTIILNILINGIIFSILFFLIILSNEEKKSILSQINRLLRVNI"
     CDS             5957..7060
                     /note="orf06"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf06"
                     /product="Polysaccharide pyruvyl transferase"
                     /protein_id="AXY99582.1"
                     /translation="MNKKKCGIVTYHRAHNYGALLQAYALKKSLQKLSLEPVFIDYQP
                     KSISKGYELYPQLPKKKSISAFIRYTKDFIHLILDYRRKKNRFIVFETFIKKEINIHK
                     PEGMFEYLFLGSDQIWNTSYTNGVDKHYYGIFDNLTANNTISYAASMGTSKLNSEEEY
                     TFIKYLKGLDGIGVREEELANYISSISSLNSEVNLDPTLLLSKAEWTSLKDNASIHVD
                     DKPYLLIYEVHEHKLTQQVKEFISKKLGLSVISLSSRTDFYTNKNYITNASPNDFIKL
                     FSNASFVITTSFHGTVFSIINEIPFLTLKFNNDTDIRSESLLRKVNLSERHIENIADI
                     DNIPLEFSFEKGNLLLEEYRKKSIDFLKSFIRG"
     CDS             7066..8220
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99583.1"
                     /translation="MKKILFVFHDSKPKSGATASMLDIINNLLKKSDLQIFALIPNTK
                     DGLYELLTNKNICVFEATLYGGRHPVPKGIKEFVKYKIRGVLKITTTYLSVLKISKKI
                     NQLDKKIDLVYSNTSDTYAGYFLSSLLKVKHVWHVREFGIEDQNCVHTLGDNQYYNWL
                     SASEKIIVISKALKYKLSKYISNDKLMLVYDDVQLADCILKNNKSLGKPLKILSVGTI
                     SEGKGQKFVIDAIENLNKKGIECSLSIVGNINSEYAKNLFDYVEKNSINNISFLGFRE
                     DIEKVRLDYDIGIISSKAEAFGRVTIEGMATGLIMVASNTGANPELISDRVTGFLYKH
                     NDLNSLLSVISYIYENRKDMQTIRENAIKFANKFDSGIASKKIYNLMKNL"
     CDS             8230..9081
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99584.1"
                     /translation="MSKNILSIIICFYPDIKHLALMSKSLTKSSNVLIIDNTPEYIEF
                     SDIDAEIIINGDNLGIAEAQNIGLRISLERGYKYSILFDQDSIVPEDMIPNLSIKLDN
                     IEKAACIGPRVFDLNCSQKMRSRVMLNQKEENNITKVNQIIASGKLINNSILSDIGFM
                     ESELFIDAVDHEWCWRAISKGYDIYIDEDTVMSHVLGENSYNFKLFKLTENSPIRTYY
                     ILRNYLLLIRRPYVPLYWKLRNFIILPTSIFLSLIFFKGRIKRLSSVFLALRDGLIGK
                     TGKIHDN"
     CDS             9071..10249
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99585.1"
                     /translation="MTINILKIKKQSLISILFFLLCFVLTRQFPLSLYIPSILSITLF
                     GFISIFFILYSNQIKFDHRLILLLIYISLLLALSLVNSNDINMLPRFLIILFLINFVL
                     LLPFYKTNLTTAFNLALSIQAIFLISFEIYLILFFSGKDYTIIRQFFLSHNLGDVYTY
                     SGYFFRIQIIGNALMPFAFMVSYFKFRVENGKFLFVILYFFACVIAANLMFLIAISLY
                     IPLIEIIKSKARIKLSIRNFLIFLLILIFFPFIIEIIYNNILLKLIGGESSSLGTRFD
                     QINALMIGLADSYYNLLFGQGLGSKLNIITTVRDYRAFIYYELQSLYILYQIGFLGFL
                     SLIVLHSYLFLYICKNKFIILIYCMYIFYAITNPYIFDTTQVIVLIYLVSLSKYFKSI
                     "
     CDS             10411..10935
                     /note="orf09"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf09"
                     /product="hypothetical protein"
                     /protein_id="AXY99586.1"
                     /translation="MMLIKIINQIIFKLFKKDILRILYISTLKNRNIFSVMSDAKEFA
                     ENIDYNQKVLDIINTYKTQGYQIILLSASIEPVVTAICHYLSYDGCFSSKLNILNEKY
                     TTGISLDLLDVKDEIIKDKYNNRKRHLVMISDNFGDSKCIPYLDEYIPVILNKKSKSF
                     WKQFNLKNSIDLRK"
     CDS             10938..11663
                     /note="orf10"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="hypothetical protein"
                     /protein_id="AXY99587.1"
                     /translation="MNFFFIPLFYFYHSRLRTLIKTISWFFIYIIPIFILAFFSGIVS
                     LNELAFLFLFIIGIYNIYEIGYINNDCETIKKEKNPTLRLSKPEIHYYNKKKPFIYSV
                     RILISLVIGVILSLNEKISIYYLLFSYIGLLISFLFYNSIRSIVNLPIHFLLVLLRFS
                     SFSLVFNLGYECFIFSALIFPLINLIERSGEARFKLTFFSKRYFSKSRFIQSPILFYF
                     NSLFLYFQYIWSRIIYYNIYAIL"
     CDS             11863..12528
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99588.1"
                     /translation="MINFVENSGFIYKSIHSQKPVGLAKGLNKLISVLENEKYVFRMD
                     ADDLSLPNRLQYQIEHMENNPNIDISGGSIQEFIGSSNNIVATRCYPLTHEGIFKVIY
                     KASPFAHVTICFRRTSLAKIGLYPVDYPLNEDIALWFQALKNGATCGNIEQVLVKVRM
                     DGAYDRRSSTKAFNEFKVYWNIMLWKKSFSFFPFMRFVFRFLPIKFVKVIYNSFVRKL
                     VTK"
     CDS             12933..14099
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99589.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFLATKELDFTATLDKEFAYKDADFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNAKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLNEFKAISDVIITNRMSSVLNDVEEKVYTRDLFGND"
     CDS             14111..14614
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99590.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(14690..15247)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXY99591.1"
                     /translation="MALTEVTFSELKACLHAEYIGDNKPFSWIRLFHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFLKKLGIYIGAKNNRKYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKDGKADIRVGNHVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYLTKVVAEIKQLSM"
     CDS             complement(15247..16080)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99592.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDDSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWGQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHAVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(16173..17198)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99593.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDAEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEIA
                     ASVLSLF"
     gene            complement(17198..17335)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     6126 a   2717 c   2680 g   5812 t
ORIGIN
        1 gactccatta acacgcttac aattagccag tgcattgtta cgtcgccgca acggagaaag
       61 taaagaatta gaacgcattg aaactgagac tcaacgcctt gatgggatga ttaatgactt
      121 attggtgctt tcacgtaatc aatataaaaa tgagttgtta cgagaaactg taaaagcaaa
      181 tgagttgtgg gatgatattt tagataacgc taaatttgag gctgaacaga gtaataagac
      241 attacaagtg acaacacctc ctggtgcatg gcctatttac tgtaaccctt actcactcgc
      301 tagcgcattt gaaaatattg ttcgtaatgc cttacgttat tcaaattctc gcattgaagt
      361 tgcattcaca gagcaaaatc aaggcatcac cattattgtt gatgacgatg gcccaggtgt
      421 tagccctgaa gacagagagc atatcttccg accgttttat cgcactgacg aagcaagaga
      481 tcgcgaatca ggtggtacag gcttaggatt agcgattgtt gagacagcag taagtcaaca
      541 tcgaggccat gttaaagccg atgatagccc attaggtggg ctgagagtag agatttggtt
      601 gccgagggga agtgccggta ataaatagcc aatagcctag atagagtaat taaactgttg
      661 tatcaaaata tcctgccatc tattgaagaa taggtggcat attctttttg ttagcatcta
      721 atgatgcaat tcagaatgat ctggctgaat cgaccgctat tccgatattt atatatctac
      781 aatcaatgct aaaataccca gctattactt gaccgtctgg tttgaagagt ctactctcag
      841 tgcggttttt cgcatattgt taaaatgtgc ttatataaca aaactcttat attacaaaaa
      901 gatgagggtt tttcgagata agttttggcg ctctgggggc cgtaaaccca gtgcggtagc
      961 tatgtatatt caatgctata aatataatat attaatatta ggttaattaa agaactaaat
     1021 aatgaaaaat tatattattt ggaaaaaatc cgacaagtag gttgggaatt acagcgttct
     1081 ttagttccat taggaaccgt aattgcttta gattctaaat caacagaata taacgctgat
     1141 ttcttattcc catccaagct agaaaaaact attttatcca tcaaacccga tgttattgtc
     1201 aatgcggctg cttatacaaa tgtagataaa gctgaaacgg atgacattaa tgcattcaaa
     1261 atcaatgctg aaagcgttga aataattgca aaaacagctg aaaaaattgg tgctctatta
     1321 atacattatt caaccgacta cgtttttgat ggaactaatg atactccttg gttagaaaca
     1381 gatactccag cgcctttaaa tatttacgga aaaagtaaac tagcgggcga acaagcaatt
     1441 attagtaact gtaaaaaata tctaattttt agaactagtt gggtttacgc aagtagaggg
     1501 aataactttg caaaaacaat gctcaaacta ggaagaaaat atgactcact gtcagtaatt
     1561 aatgatcagt atggagctcc cacaagcgct gaattattag ctgattgtac tgctcatgca
     1621 attaaaagta cattagataa tgggaagcca ctttatggtt tgtatcattt aacagcaaaa
     1681 ggaacaacca cttggtttga gtatgcatca ttaatattta aatatgcacg agaatactca
     1741 ttacctttag tagttaaaaa tttaactcca attcctgcca cagaataccc tttaccagca
     1801 tctagaccat ataactcccg tttaaacatc caaaaattcg aaaaaaactt cagattagaa
     1861 ttacctaact ggaaatttgg catatataga atgctaaacg aaatttcaga taacaagtaa
     1921 acactaattc ttacgaactt ataaaatgaa aaaatataaa ggcattattc ttgctggcgg
     1981 ttcaggaaca cgactttatc ctgtcacaat ggcagtaagt aaacaattat tacctatcta
     2041 tgataaacca atgatttatt atccgctcag tacacttatg cttgcgggaa tacgggatat
     2101 tttaatcatc agtacacctc aagatactcc ccgttttcaa caattattag gtaatggaaa
     2161 tcaatgggga ataaatctcc aatataagat acaacctagt cctgatggtt tagcccaagc
     2221 atttattctc ggtgaggatt ttattggttc agataattgt gctcttgttt taggcgataa
     2281 tattttttat ggtcatgatc ttccgaaaca attagaacaa gcgattcaga aagaacacgg
     2341 agcgactgtt tttgcatatc atgttaatga tcctgaacgc tatggtgttg ttgaatttga
     2401 taagaataaa attgctattt ccttagaaga aaaaccatta aaacccaaaa gtaactatgc
     2461 agtcactggt ttatatttct atgacaatga tgtcattaat attgccaaaa atttacaacc
     2521 ttctccgcgt ggtgaattag aaatcacaga tgtaaataaa gtatatttag aaaaacataa
     2581 actttctgtt tctataatgg gtcgtgggta tgcgtggtta gatacaggaa cacataaaag
     2641 tcttattgat gcaagcaact tcattcaaac tatagaagac agacaaggat taaaagtttc
     2701 ttgtccagaa gaaattgctt atagaaaagg ttttatcaat aaagaacaag taagaatact
     2761 tgctgagcct ttatctaaaa atgattatgg cagatattta ttacaaataa ttcagagtga
     2821 ttaattatga atattataaa aacagaaatt cctgatgtgt taattttaga gcctaaagta
     2881 tttggtgacc atagaggttt tttctatgaa agcttcaatc aaaaaatctt tgaagaggca
     2941 gttggttatc cagtaaattt tgttcaagat aatcattcaa aatcttcaaa gggtgtatta
     3001 agaggattgc attatcaaaa agctccttat gctcaaggaa aattagttag aactgtttta
     3061 ggtgaggttt ttgatgtagc tgttgatata agacctgaat ctagtagtta tggtaagtgg
     3121 gttggtgtaa ctttatcagc aaaaaataaa cgtcaattat ggattcctga aggttttgct
     3181 catggttttt tagttttaag tgacgaggta gaatttcttt ataagacaac caacttttat
     3241 caccaacaaa gtgaatctca tattgtctgg aatgatcctg atttaaatat aaactggcca
     3301 attaaagatt caccaatatt atcggaaaaa gataaaactg gattatattt taataaatta
     3361 gttggaatgt aaaatgaagg aaaaaatatt attacaactt gatcattggc tcgataaaga
     3421 atgtatttta tattatatag attatgattt aaaaaataat agctattcaa aaacaggatc
     3481 catagtaaaa aggtttgttt tacctaaaaa tcatgaacaa ttaataaatg ttatatgttt
     3541 ttctaagagt atagatcttg atataaaaat catcggtgaa acaacaaata tattatttaa
     3601 tgatacagta aactatggtg tgtttatatc tacaaaatta gttaataatg ttcaatttaa
     3661 agatagtaca gtaatcgtag aatcaggcaa atccttacct gactttgttc gtgatgttag
     3721 tatgagaggg tatggagggt atgaaggact agaaggtatc ccgggtagta taggtggtgg
     3781 aatcgtaatg aatgccggtg cctatggtta tgaaatatcc gataatatta tcagtgttac
     3841 ttatttaaat gaaaatgggg aagtaattac caaagataaa tcaagcctaa attttgaaaa
     3901 aagaaattct atttttaaat ataaagacct aattattctt tatgccgaat ttcaaatatt
     3961 acgttcccca ttagaagata taaaaaataa aatagaaact ttccatatag caagacatat
     4021 ctatcaagag tggacaatgc cgaatctagg gagtattttt actgccccaa gaagcttata
     4081 tgaccatttt aaatttagtt catttaaaaa cgaactctca tataaactga caaaaaaaat
     4141 attttacaat aagattatgc gattttttaa tcgaaaaaaa ccaagtaatg aacgattaaa
     4201 caatataata aaaatagata aaaatctaga tttgcttaca gtaaattgtt ctcataaaaa
     4261 tataaataca ttttgcaacc aaaataactc tacaagagaa attattgatt atatatactc
     4321 aatttataac tcaatagata aaaaaaatgt atttcttgaa aatgaattag tggagaaaag
     4381 catatatgaa ataatatctg aaaatgacta caaggatatt acttttaaat ataaaaaaat
     4441 actagaaaat gaaaagattt agcaatgtat taaaaaattc tgtacttcta tattttagaa
     4501 tggtcgtagg actattcgtt tcactttata tatcaagaat aagtttagat atccttggtg
     4561 ttgatacttt tggactttat actataatag ctagttttgt cattgttggt ggtgtgctag
     4621 tcaatgttgc aacagtatca atgcaaaggc atttatcaga aaaaatagga gaaaatctca
     4681 gtagaacttt aagtacagaa tctaaaaatg ttctagatgc ttgtgttatt gttaatacaa
     4741 tgcttggtat attctctata ttattaataa tcttatttgg aacagtatat atcaatttat
     4801 atctaaacca agacattgtt aatataagta ttatctggta tgtgttttac gtatccatcg
     4861 ctacattttt aatctctttt acaacatcac cttatctaac actattgatc tcttttgaag
     4921 atactaaata ttatgctcta ataatgatat tggaaattat aagtaagcta atttctatac
     4981 tttgcttata tttaacaggt tcaaaaagca ttttagatta caccattgcc atattcatat
     5041 ctatactaat tactagatta atactatttc taatagtaaa aaatagatat aaaaatatac
     5101 ccctaacttt gacctatgat tggaatatta ttaaacctat cctttcattt actggttgga
     5161 atttatgggg aggaatagct tctgtaatgt ctcttcaagg tgttactttt ttaataaata
     5221 tattttttgg cttatcagtt aataccgctc gagctatatc tttacaaata tactcagcag
     5281 taacacaatt agtaaacagt attcagatcg ctattgctcc ccagctagtt aaatcaaatg
     5341 gcaaagaaaa taactatttt tctcaacttt ttatacttag cgggaaaata acaatttatc
     5401 ttgcattttt gatttcgctt atattatatg aaaacactga gagtctttta gacttatggc
     5461 ttgataatta tcctcctgtt acaattatat ttctcaaatt tacatatatt gaaattattc
     5521 ttacttcctt aggttatcca gttttatcag ctgttcaagc aaatggtaat attaaaaaat
     5581 atcaattagt tgttggcggt ttaatgatat taaatattcc ttttatattt atttcgataa
     5641 gcctatatga aacatatata tctatatata tagttagtat tattatatca tccatctctc
     5701 tactatatag acttatctat gccaaaaaaa ttatgggctc gattgtcaat gagtacttat
     5761 tttcagtagt cattaagggt atattagttc tattactatc cttaatacta actaatttaa
     5821 ttcattgtaa tacaataata ttaaacattc tcataaatgg tattatattt tcaatattat
     5881 tcttcttgat aatactatct aatgaggaaa aaaagagcat attatcacaa attaataggt
     5941 tgctaagagt taatatatga ataaaaaaaa atgtgggatt gttacatatc atcgagctca
     6001 taactatggt gcattattac aagcatatgc gcttaagaaa tcattgcaga aactatcttt
     6061 agaaccggta ttcattgatt atcaaccaaa atcgatcagt aaaggatatg aattataccc
     6121 tcaattacct aaaaaaaaat ccatttcagc ttttataaga tatactaaag actttataca
     6181 tctaatttta gattatagaa gaaaaaagaa taggtttatt gttttcgaaa cctttattaa
     6241 gaaagaaata aacattcaca aacctgaagg catgtttgaa tatttatttc ttggtagtga
     6301 ccaaatttgg aatacttctt atactaacgg cgttgataaa cactactatg gtattttcga
     6361 taatttaaca gcaaataata ctatatccta cgcagccagt atgggaacat ctaaattaaa
     6421 ttcagaagaa gagtatactt ttattaaata tttaaaaggt ttagatggta ttggtgttcg
     6481 cgaagaagag cttgcaaatt acataagctc gattagcagt ttaaactcag aagttaatct
     6541 agatccaacg ctactactat ctaaagcaga atggacttcg ttaaaagata atgcaagtat
     6601 tcatgtagat gacaaacctt atttactaat atatgaagtt cacgaacata aattaaccca
     6661 acaagttaaa gagtttatat caaaaaaatt aggcctaagt gttatatcat tatcttctag
     6721 aacagatttt tatactaata aaaattatat cacaaatgcc tcaccaaatg attttataaa
     6781 attattttca aatgctagtt ttgttattac gacatctttt catggtactg tattttcaat
     6841 aataaatgaa attccttttt taaccctgaa atttaataat gatactgata tacgaagcga
     6901 gtcactatta agaaaagtaa acttatcaga gagacatatt gaaaatatag cagatattga
     6961 taacatccca ttggaattta gctttgaaaa aggcaatctg ctgcttgaag aatataggaa
     7021 aaaatcaata gactttttaa aaagttttat ccgaggctag ttatcatgaa aaaaatatta
     7081 tttgtttttc atgattctaa acctaaaagc ggtgcaaccg cttctatgtt agatataata
     7141 aacaatttat taaaaaaaag tgatctgcaa atattcgcat tgattccaaa tactaaagat
     7201 ggattatacg aactattaac taacaaaaat atttgcgtat tcgaagcaac tttatatgga
     7261 ggaagacatc cagtaccaaa aggaataaaa gaatttgtaa aatataaaat aagaggcgta
     7321 cttaaaataa ctaccactta tttatccgta cttaaaatat ctaaaaaaat caatcaacta
     7381 gataaaaaaa ttgatttggt atattctaat acttcagata cttatgcagg gtatttttta
     7441 agtagcttac tcaaagtaaa acacgtatgg catgtgagag aatttggaat tgaagaccaa
     7501 aattgtgttc atacacttgg tgataatcaa tattataatt ggttaagcgc ctcagaaaaa
     7561 attattgtca tatccaaggc ccttaagtat aaattatcta aatacataag taatgataag
     7621 ctgatgcttg tttatgatga tgtccaatta gctgattgca tattaaaaaa taataaaagt
     7681 ctcggaaaac cgctaaaaat attaagtgtg ggaacaattt ctgaaggtaa aggacaaaaa
     7741 tttgtaattg atgcaataga aaatcttaat aaaaaaggga ttgaatgctc tctttctatt
     7801 gttggaaata ttaactccga atatgcaaaa aatctttttg actatgtaga aaaaaattca
     7861 attaataata tttcttttct tggtttcaga gaagatatag aaaaagttcg attagactac
     7921 gatattggta ttatatcctc aaaagctgag gcatttggta gagtaactat tgaaggtatg
     7981 gccacaggat tgataatggt tgctagcaac acaggtgcga atcctgaact aattagtgat
     8041 agagtaactg gttttttgta taaacataat gacctaaact ctcttttaag tgtgatctcc
     8101 tacatatatg agaatagaaa ggatatgcaa accataagag agaatgcaat taaattcgct
     8161 aataaattcg actcaggaat agcaagcaaa aaaatatata accttatgaa aaacttgtaa
     8221 tttactacta tgtcaaaaaa tatattatct ataataattt gtttctaccc ggatataaaa
     8281 catttggctt taatgagtaa atcattaaca aaatcatcca atgttttaat cattgataac
     8341 acgcctgaat atatcgaatt ttcagatatt gatgctgaaa taattataaa tggtgataat
     8401 cttggtattg cagaagcaca aaatattggt ttgagaattt ctttagagcg tggttataaa
     8461 tattcaatat tatttgacca agatagtatt gtaccggaag atatgatacc taatctatct
     8521 attaaacttg ataatataga aaaagcagcc tgtattggtc caagagtatt tgatttaaat
     8581 tgttctcaaa aaatgcgttc acgtgtaatg ctaaaccaaa aagaagaaaa caatataaca
     8641 aaagtaaacc aaattatagc ttcaggtaaa ctgataaata atagcatact ttctgatatc
     8701 ggtttcatgg aatcagaatt attcattgat gccgtagatc atgaatggtg ttggcgtgcg
     8761 ataagtaagg gatatgatat atatatagat gaagatacag taatgtcaca tgttcttggt
     8821 gaaaactctt ataacttcaa attatttaaa ttaacagaaa attcacctat taggacttat
     8881 tatatactta gaaattatct tttacttatt agaagaccct atgtaccttt atattggaaa
     8941 ctaagaaatt tcattatctt gccaacatct atttttctga gcttaatatt ttttaaagga
     9001 agaattaagc gattaagtag tgttttctta gcattaaggg atggtttgat cggtaaaact
     9061 ggtaaaatac atgacaatta atattttaaa aataaaaaaa caaagtttaa tatctattct
     9121 tttcttttta ctttgttttg tccttacccg tcagttcccc ctttctttat atataccatc
     9181 aatattatct attacattat ttggtttcat ttccattttt tttatacttt atagtaatca
     9241 aataaaattt gatcatcgac taattttact actcatatat atatcattgt tactcgcttt
     9301 atccttagta aatagcaatg atattaatat gttacctcgt tttttgataa tattattctt
     9361 gatcaacttt gtacttttac taccttttta caaaacaaat cttacaactg catttaattt
     9421 agcattatct attcaagcta tctttttaat tagttttgaa atttatttaa tattattttt
     9481 ttctggtaaa gattacacca ttattaggca attttttctc tcgcataatt taggcgatgt
     9541 atatacatat tcaggatatt tttttagaat acaaattatt ggtaacgctc tgatgccttt
     9601 tgcctttatg gtatcttatt ttaagtttag agtagagaat ggtaaatttt tatttgtaat
     9661 attatatttt tttgcttgtg ttattgctgc aaatttaatg tttcttattg caatttctct
     9721 ttatatacct ttaattgaaa ttattaaatc taaagctagg ataaaattat caattcgaaa
     9781 ttttcttata tttctattaa tattaatatt ttttccgttt ataattgaaa taatatataa
     9841 caatatatta ttgaagttga ttggaggtga atcaagttct ttaggtacga gatttgatca
     9901 aatcaatgca ttaatgattg gcttggctga tagttattat aaccttttat ttggacaagg
     9961 attaggtagt aaattaaata taataactac tgtgagagat tatagagcat ttatttacta
    10021 tgaacttcaa tcactttata ttctttatca aattggtttt ttaggtttct tatctcttat
    10081 agttttacat tcctatttat ttttatatat ttgtaagaat aaatttataa ttttgattta
    10141 ttgcatgtat attttttatg caataacaaa tccatatata tttgatacaa cacaagttat
    10201 tgtattaata tacctagtct ctttatcaaa gtattttaag agcatttgat ttagagtagt
    10261 ctataattgg atgtatacaa gtcatgaggc tgacttttgg acaaaatatt atttgttgac
    10321 atttgtcata caatagttcc ccataacact acaattttat tcttagaaca atactacata
    10381 aaaagtaatt tcttaaaaag aataaggcgt atgatgttaa taaagattat caatcaaatt
    10441 attttcaagt tatttaaaaa agacatttta aggatcttat atatttccac attaaaaaat
    10501 agaaatatat tttcagtaat gtcagatgca aaagaatttg cagaaaatat tgattataat
    10561 caaaaagtgc tcgatattat taatacgtat aaaactcaag ggtatcaaat cattcttctt
    10621 tctgcatcta ttgaaccagt cgttacagca atatgccatt atttatctta tgatggatgt
    10681 ttttcatcaa aattgaatat tttaaatgaa aaatatacta caggaatctc tttagatcta
    10741 cttgatgtta aagatgaaat aataaaagat aaatataata atagaaaaag acatttagtg
    10801 atgatttcag ataattttgg agattctaaa tgtattccat atttggatga atatattcct
    10861 gtaatactta ataaaaagtc aaaatcattt tggaaacagt tcaacttaaa aaatagtatt
    10921 gatttgagaa aataattatg aattttttct ttatacctct cttttatttt tatcactcaa
    10981 ggctaagaac cttaataaaa accatatctt ggttttttat ctatataatt cctatattta
    11041 ttcttgcttt ttttagtgga atagtctcac taaatgaatt agctttttta tttcttttca
    11101 ttataggaat atataatatc tatgagatcg gatatataaa taacgattgt gaaacaataa
    11161 aaaaagaaaa aaatcccaca ttaagacttt ctaaaccaga gatacactat tacaataaaa
    11221 aaaaaccatt catatatagc gttagaatat taatttctct agtaataggg gtaattttat
    11281 ctctaaatga aaaaatatca atttattatt tactattttc ctacatagga ctattaatat
    11341 catttctttt ttataattct ataagatcta ttgttaatct tccaatacac tttttactag
    11401 ttttattaag attttcatca ttctctctag tttttaattt aggatatgaa tgttttatat
    11461 ttagcgccct tatttttcca ctaattaatt tgattgaaag gagtggagag gcaaggttta
    11521 aactaacctt tttttcaaaa aggtattttt ctaaatcgcg atttattcag agtcctatat
    11581 tattttattt taactctttg tttttatatt ttcaatatat atggagccgc attatatact
    11641 acaatatata tgctatctta tagaatcacc tcaccattaa tttttagtat tattaaaaag
    11701 agttagttat gtttagtatt gctgttatta ttgctgtata taaaaatgat aaatacgagg
    11761 attttgttaa tgctattaaa tccataaaac tttaatcaag aatccattca acaagataga
    11821 attaaaatct atttacatat agatggtcat gtcagtaaaa acatgattaa ttttgttgag
    11881 aactctgggt ttatatataa atcaattcat tctcaaaaac ctgtaggctt agctaaagga
    11941 cttaataagt taatttctgt tttagaaaat gaaaaatatg ttttccgcat ggatgcagat
    12001 gatctatcat tacctaatag acttcaatat caaattgaac atatggaaaa taatccaaat
    12061 atagatattt ctggaggctc tatacaggaa tttataggct cttcaaacaa catagttgca
    12121 actcgttgtt atccattgac acatgaagga atttttaaag ttatttataa agcatcacct
    12181 ttcgctcatg taacaatttg ctttcgtcgc acatcattag ctaaaatagg tctctatcct
    12241 gtcgattatc ctttaaatga agatattgct ctctggtttc aagctttaaa aaatggtgca
    12301 acatgtggaa atattgagca agtgctagtt aaagtgcgaa tggatggtgc atatgataga
    12361 cgatcttcta ccaaagcttt taatgaattt aaagtttact ggaatattat gctatggaaa
    12421 aagtcattta gcttttttcc ttttatgcgc tttgtttttc gatttttacc cattaaattt
    12481 gtgaaagtta tttataattc ttttgtcagg aaattagtca caaagtaata ttctttgatt
    12541 aataacttta ggtatttatg caatatttaa ttttattttt aataaagaag attatattag
    12601 ttttttaaaa attaactaca attttattaa ttatctataa ataatgtaaa tcagaaactc
    12661 attcccttta acttacttaa tttttaaaaa tcatctacct tccgaaaaat gaggaattta
    12721 taattacgag tgctcctagg aaggatatta ataccaatta aagaaaatat ataatttaat
    12781 tgatactatt agtagattta taaaattatc ctatgacaat aaattagaaa tgagtaaaaa
    12841 taacaggaag aaaattgaat ctgaacttaa tgaaagcatt attaataagt atataaacac
    12901 attcaataaa tttcatagtg ttagggtaaa aaatgaaaat cacaatctca ggtaccggct
    12961 atgtcggtct atctaacgga atacttcttt cccaaaacaa caaagttgtt gcgttagata
    13021 tcaataaaga aaaagttgaa ttactgaata aaaaaatctc tccaattaca gataaagaaa
    13081 ttgaagaatt tttagcaacc aaagagctcg attttactgc aaccttagat aaagagttcg
    13141 cttataaaga tgcggatttt gttatcattg caacacctac cgattacgat cctaaaacaa
    13201 attactttaa tacctcctct gtagagtctg ttatccgtga tgttttagaa tataatccaa
    13261 acactacaat gattataaaa tcaaccattc ctgttggttt tacgaaagaa atgagagaaa
    13321 aatataatac ggataatatt attttctctc ctgaattctt acgtgaaggt cgtgcacttt
    13381 acgataattt atacccatcg cgtattgtta ttggtgaaca atcagaaaga gcacaaaaat
    13441 ttgcagacct tttacaacaa ggtgcattaa agaaagatat tgatgttctg ttcactgact
    13501 caacagaagc agaagccatt aaattatttg ccaatactta tttagcacta cgtgttgctt
    13561 actttaatga attagatagc tacgctcaag cttatggctt aaatgcaaaa caaatcatcg
    13621 aaggcgtctg cttagatcca cgtatcggta atcattataa taacccatca ttcggttatg
    13681 gcggttactg cttaccaaaa gatactaaac agctgttagc aaactacgac tcggtgccta
    13741 ataatattat tagtgcaatc gtcgaagcta accgcacacg taaagacttt attgccgact
    13801 caattatcgc taaatcacca aaaatcgtag gtgtttatcg tttaattatg aaatctggct
    13861 ctgataattt ccgttcttct tctatccaag gcattatgaa acgcattaaa gccaaaggga
    13921 ttgaggtggt aatctatgag ccagagatga aagaagagac tttctttaac tcaaaattaa
    13981 ttcgtgattt aaatgagttt aaagcaatct ctgatgtcat tattactaat agaatgtcat
    14041 ctgtactaaa cgacgttgaa gaaaaagttt atactcgtga cctctttggt aacgactaaa
    14101 tcgattttat atgctcaata tcgtcttatt tgaacccgaa attccaccga atacgggtaa
    14161 tattatccgc ctgtgtgcta acacaggctt tcatcttcat ttgatccaac ctttgggttt
    14221 tacttgggat gataaacgtc ttcgtcgtgc aggacttgat tatcgtgaat ttgctgatat
    14281 taaacagcat cacgattact atgctttttt agaaagtgaa ggtttaaacc ctgataatgc
    14341 acaatcacca acgggtgcgc gtgtatttgc gttaacgaca aaaggtacac cagcacatag
    14401 caatgtaagc tatcaagatg gagattatct cctttttggc cctgaaaccc gtggtttgcc
    14461 accttatgtg ttagataata tgccaacaca gcagaaaatc agagttccaa tgcttgctga
    14521 tagtcgcagt atgaacctgt caaataccgt tgcagttgtg gtttttgaag catggcgtca
    14581 acttggttat cctggtgcgt tattaagaga ctgaaatgca aaaagcgctg atcataaata
    14641 atgagcaagc gcttttttaa tttcaaatga ctaaaaactt aatttattat tacattgaga
    14701 gttgttttat ttcagcaaca accttcgtga gatacctaca cccttcccct acatccttca
    14761 ataccaatgc gtgagcacca atcactgcat tatcaccaat ctctatctca ccgccgataa
    14821 tcgaagaatt acaaccaata taaacgtggt tgccgactcg aatatctgct ttaccatcct
    14881 tttcttttac tccgatggtc acaccttgca ttaacgtcac attctcacct aattgacagt
    14941 aattagtcag cacaataccg ataggatgtg gtaatgaaag gccttttcct atgcgcgttc
    15001 ttaatgagat atcattgcaa tatttacgat tgtttttagc accaatgtaa atgcctaatt
    15061 ttttgagaaa acgattttta gagaaataaa aaaattgagc gacacgccac caaaacagat
    15121 aacgtgaacg ttggcagaaa aagacgcgat gaaaaagtct tatccaacta aaaggcttgt
    15181 tatcaccgat atattcagcg tgtaaacaag ccttaagttc agaaaatgta acttctgtta
    15241 atgccattaa atgccatcac cacattcaaa accaggtact acaccattaa agtgttgatc
    15301 catatcgagt gaaggtttat cactcgtcgg cttaccaaca atgcgagcag gaacccccgc
    15361 aaccgtggta tgtgcaggca ctgcatgtaa tacgacagat cccgcaccaa ttttagcacc
    15421 gcgaccaatc tcaatattgc cgagtatttt agcacccgca ccaatcatca caccttcacg
    15481 cactttagga tgacgatcac cacaggtctt acctgttccg cctaaagtca cagattgtaa
    15541 aatagaaaca tcattctcaa caacagctgt ctcaccaatt acaatgcctg ttgcgtgatc
    15601 aagcataata ccgtggccaa tacgtgcagc tgggtgaata tcgacaccaa acgtaacaga
    15661 aatctggttt tgtaggtaaa cagcaagcgc tttgcgatct tgtccccata accaatgtgc
    15721 tatgcgataa gcctgcaacg catgaaagcc ttttaaataa agcaacggtg tggagtattt
    15781 atcaactgcg gggtcgcgta aacgaacagc tgaaatatca taagccgcag attcgatcat
    15841 ggaatcatcg ctacgatacg cttcttcaac aatctcacga acggcaatcg caggcattgt
    15901 ctgtgtcgcc agcttattcg ctagcatata acttaaggcg ctacctaagt tttcatgttt
    15961 gagtaatgtc gcatggaaga aactcgccaa aatcggctcg caatcagcca aacatcttgc
    16021 ttcattttta atatgatccc agactctttt tagttcttct agcgacatac tcactctcat
    16081 tctttttaca ttgaaaccta agtaacaact taagcttctt atgttgaaaa ccgaaacaga
    16141 cactttaaac caatgcgtta agtagcgatt atttaaaata gacttagaac agaggcggct
    16201 atttcatctt ttgttgcgcg acctaataaa gatagtgccg cctctttcgc gtctttatgt
    16261 tggtataaaa tttggtagat ctgctcagtg ataggcattt caacaccgac ttgttctgct
    16321 aaggcgcgaa cttctttagt gttccgataa ccttcaacca cctgcccgat tttctcttgg
    16381 gcagtatcaa catcaagccc ttggcctaac atcataccaa atcggcgatt acgggattgg
    16441 ttatcggtac aagttaaaac gagatcgcct aacccggcca ttcccataaa cgtcgcagca
    16501 tctgcaccta atgctttacc taaacggctc atttcagcaa gtccgcgggt aattagtgct
    16561 gtacgtgcat tcgcgccaaa ccccatacca tcagacattc cagcaccaat agcaatcacg
    16621 ttttttaccg cgccacctaa ttgcacaccg ataaagtcag gatttttata cacacggaaa
    16681 cttttaccac aatggaacaa ctgttgaagc tcttcagaaa atgtattatc tgttgatgca
    16741 acagatatcg cagtaggcag acctgctgca agctctttgg caaaagtagg accagataaa
    16801 actgcgagcg ggatttcatt acctaatact tcacgcgcca catcttgtaa caagcgacca
    16861 gtatgagcct caagtccttt tgtcgcccaa acaacacgtg catcctgacg taaaaagggt
    16921 ttgatctgct gtaatacgtc accaaaaaca tggcttggga tcacaaccag aatattacgg
    16981 ctagcttcaa tggctttttg caaagaagct tccatgtata aactatcagg aaaagaaaca
    17041 tcgggcagaa atgcctgatt acaacgtgct tgttctaacg ccgcaacatg ttcagcatca
    17101 tgaccccaaa gtacaacatc atggccattg cgtgctaagg taatcgctaa agcggtgccg
    17161 tatgaaccgg caccgataac tgtcatagaa gcgttcatta agcttcttca gccccttcat
    17221 tagtagcatc agtttgttgc tgttgtaaat agttcataaa caacgcatca aagttaactg
    17281 gtgctaagtt cagttgtggg aaagtaccac ggctgactaa gtttagtgat acatt
//
