LOCUS       KY710703               15588 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 49/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710703
VERSION     KY710703.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 15588)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15588)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15588
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 49/57"
                     /serotype="O26"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..15588
                     /note="O antigen gene cluster"
     gene            3..677
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             747..1640
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99594.1"
                     /translation="MNKYLKIIRNKLRYKYCDVFPKLITKSIYKERMNKKLDLRHPQT
                     FNEKLQWLKLNLYRDNPLVTQCADKYAAREYVKECGCQEILNEIHQVWEEPHEINFSE
                     LPNKFVLKCNHGAGYNIICRDKNSISPDKIKQKLSTWLNEDYWRLSVEFVYKDVPKKI
                     ICEKFIETKNNELPYDYKLFCFHGKPLFVMICTERESHHPKFYFVNKEWELLPYGIDY
                     KNSNVSTVLEKPKNYQKLFEYAEKLSKPFPFVRVDLYLNNGIIHFGELTFIPSAGMDK
                     ELNNEEVKNVDIIFGNYLKLN"
     CDS             complement(1652..2641)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99595.1"
                     /translation="MKNHPKISVLMSVYNDAVHLEEAINSILNQTVKDFEFIIIDDGS
                     TDVSVDILRRYQEQDSRVKVYTNESNIGLAKSLNKGFLLSSGEYIARMDADDYAYPTR
                     LQKQLEFMEAHKNIMVCGTSMEIYEDRSKFMVPPTTHNAIAAGLIFGTNFYHPTVMFR
                     AKFIEDNQITYPEDYKYAQDYGLWVNLLKLGKKDIFANLEEPLLKYRTHPEKNRKKYF
                     NTQTSFAIKTQISALEIFSINENEKVLYDLNFSSEYLTLDNIKKCNSLLEKIRIKNIQ
                     SKPYFYKELKIAIIRKKVSLFKRCEKKSFYYPYMKLKSRFIYLINRKIIKSIN"
     CDS             3311..4195
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99596.1"
                     /translation="MNKPKHPLSLRVIYFLAKRNFLSFLSDSLYLKIIFYLHRGEKLD
                     LQNPKTFSQKIQWLKVNIKDPQLHILVDKFLVRDYIKNKIGCDILIPMLGIYNNVDDI
                     NFNVLPDQFVLKTTHGSSQNIICKDKKNLDINKTKAKFKKWEKVCLYKIFKEWAYKDV
                     TPRIICEEFIGLNNNVPNDYKFYCFNGKIGFIQVNKERFSNNHTETFFNENWEMLDFN
                     YGVEQSTSMIEKPENFLEMKEIVRVLAKEHKFIRVDLYNVNNKIYFGELTFYPASGLE
                     FMNPESLERTFGDMLDLS"
     CDS             4192..5394
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99597.1"
                     /translation="MNIKGNIIYMYIAYLFNMLFPLIIVPLLTNNLSLEDYGKYSLVY
                     TCFSFAIILSDFSFNITGIRYYLREKNDYNKNFIYQKIQSSKKIISYISTTILTLYIH
                     YIYNDVYTSVIFFISTLFGLHGYINFSSWYFQAIGQLRNNTIYLIIMKFISLIIIVTL
                     IKLNNNNWINIYACSTILYYPFGIIIQRKLKKIKIKIKIKFVFNLIKSNSGLFLADFL
                     PNLYNLIPIIILGMYIDASDYAIFSLSNRIALIICGFIYVFLKAIYPYFCTKDTINTN
                     RFILLLVFITLSGYLINYIFGKNIITFIFGNDYIYAQEYLNYIFIGIVSISISETILY
                     SYLLPKNKDNFITKTSLIIVILSVVILLLTFHAYKQWAVIYTIIFARVLYSLIYLSYY
                     SFYKNEKN"
     CDS             5408..6205
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXY99598.1"
                     /translation="MSIYQNDKKKWVCDSINSILNQSESNFHLLLSIDGYINDELKKE
                     IDFFKKHPKITVFIYNEKKGLATRLNQMIDFAISNFEFDYFARMDADDICTLDRLEVQ
                     CDFLSKNKNIDIIGSSVIEMDQNERPLNIKKMESNHDILKKNIIRKCPLNHPTVMIKK
                     DVFMNNHRYDQNLMNTQDYMLWINLMASGYKFSNIEKPLLYFRISNSFYSKRGINKAV
                     NDFTIRMIAMKKLHCFSLKNFLFTNLIFIIRISPKFIKKVAYKWGRK"
     CDS             6190..6711
                     /note="orf07"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf07"
                     /product="hypothetical protein"
                     /protein_id="AXY99599.1"
                     /translation="MGTKMKKVKRIFLEEIWSFYQWIFLFIPGRVGHYTRGFFLSFFF
                     KKHGKKITIKENVEIYHPENLIIGSGSGFGRNNVIDCIGGITIGNNVRFGPNVMIATM
                     NHAQIGTSIGSAIKEKKAVKIGDNTWIGNGVTILPGVEIGDNCIIAAGAVVTKAVRSN
                     TSVGGVPAKNLNL"
     CDS             6708..7865
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt5"
                     /product="glycosyltransferase"
                     /protein_id="AXY99600.1"
                     /translation="MKIICLLGEKFYKENNKYHYSSSSCLFINETFSEENVTFMSQVK
                     KYDNEKTVGSLEKNKFIELPFFDSNISLIKYLFNPINYIYLNKKMNFVINNNPDAIFW
                     VRNPALISIIFTSKLMKKNKFFISHFCADVKGISKIKYKKGFKYFLSYLLESYILNKM
                     DKINSYEKCVIFSTGDILNKRYNNSTHLLDISLPKKTSSINTYEDNYFLFVGRIQKDK
                     GIIQLIDAFNLFKLNNNNYKLVIVGYGKDFDEINNLIKSNSYNDIILTGPKNTIELQH
                     FYSHCHSLIVPSCNSYEGFPRVIAEAWYYGKPVILTNVGGINALAINNVNSLIIDDST
                     PSKIKNALIKITNKELYENIKNNIKNNIKLSERYYWNNLVRKIVSNLHEKF"
     CDS             7852..9036
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99601.1"
                     /translation="MKNFSLFLSLFLATLANHSAITIPLAFVFLLISLFFNKGKCYFS
                     SQKLIILITSITFIIIHSILFKLSMLNYIPAKEFIDSFEINFYKYILLASYVFIISII
                     IQHEKNELLIKILSFILIIHITFFIIQFFVVYLTGYYLDFIYPITHEHSRYLFYGVEN
                     NISIYRCTGLFVEPSTYSISIFTLLLILESLGNKNKFLFYLTLLTMILSLSSISFILI
                     PFYYLLKIIRKIKLSYLIVTILLLFSFSYFSQNNFIKTQIDKINNTSSIRFNLVNAIF
                     DRKIDLLITGSGLYGIEKNIIDGTKATCNDNETCSNESINRKYATPNDYGLLFYIFIK
                     LGFFSFIILYLICYPFITNYKKMSFFICVLITKLQFAFPLFWIFVLILRRQDSNENTY
                     RS"
     CDS             9014..10081
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt6"
                     /product="glycosyltransferase"
                     /protein_id="AXY99602.1"
                     /translation="MKILIVVKWLINGGAENNIIRIANFLNKNGFDIDILVLNDTAEK
                     PLIKVINKGIKNIFVMKKKYNFLTFVLKNKKKYKIIICGDHRIGVYFSIFKYFYLEKK
                     TKIICRCINNLDLLIGSKNFLYKKLFNYFFKKIDIIISQCKEMEKNLKNEWKVNDVIT
                     IYNPVNKSKIIKSTINNKNFIFIGRFSKQKKLDFMLKAFNLALRENNDIHLTLVGYRE
                     WMAEDKLVKKDLDSFIVNNNLQNKVNIINYSKNTFNYLLNSDIFLLTSEYEGFPNVLL
                     EANSLGLPVISVDCPFGPKEIINQDNGLLVNKESYIEFSKKIILSTQINWDPIKISNS
                     TILYDENKQYSKLINVIKGLS"
     CDS             10081..11247
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99603.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYNLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIHNLDEFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     CDS             11273..12283
                     /note="NAD-dependent epimerase/dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gla"
                     /product="gla"
                     /protein_id="AXY99604.1"
                     /translation="MKYLVTGAAGFIGFHLIKKLIQQGETVVGIDNLNDYYDVALKEA
                     RLNLLNQLDNFSFSFIDLADREKIAQLFEIEKFDRVIHLAAQAGVRYSLINPFSYADS
                     NLTGFLTILEGCRHNNVKHLVYASSSSVYGLNDELPFSPHDQANHPVSLYAATKKANE
                     LMAHSYSHLYGIPTTGLRFFTVYGPWGRPDMALFKFTKAIINNQPIDIYNHGEMKRDF
                     TYVEDIVEGVTRIADVIPTAHQDWKVSTGTPADSSAPYKVYNIGNGSPVNLMDYINAL
                     EIHLGKKADKNMLPMQPGDVYTTWADTEDLFKATGYKPQTSVDEGVKQFVDWYKNYYQ
                     VK"
     CDS             12287..12790
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXY99605.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(12920..13474)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemX"
                     /product="wemX"
                     /protein_id="AXY99606.1"
                     /translation="MQAQAKVTLAELKDCLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGNHVYIGCNSSIIGGEIKIGDNAIIGAH
                     ALVLKDVGEGCRYITKVIADIKQV"
     CDS             complement(13477..14310)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99607.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(14407..15423)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99608.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVPFLIVDIWKLLCKKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGFDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSLL"
     gene            complement(15423..15587)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5556 a   2440 c   2360 g   5232 t
ORIGIN
        1 cctgcaacgt aatggttgaa gcacagcagc gattgatttc tgatatctcc catgaactgc
       61 gcacaccgtt aactcgttta cagctcgcca gtgcactgct acgccgtcgt agcggggaaa
      121 gtaaagagct agagcgtatt gaaacggaaa cacagcggct agatagcatg atcaatgact
      181 tattagtgct ttctcgcaac cagtataaaa atgagttatt acgtgaaacc gtaaaagcaa
      241 atgagctttg gaacgatatt ttagataatg cgaaatttga ggctgaacag agcaataaaa
      301 ccctaacagt aactgcccca ccaggcccat ggacgattta ttgcaatcct tattcattag
      361 cgagtgcctt tgaaaatatc gttcgcaatg ccttgcgtta ttcacattct cgtattgaag
      421 tggcctttac cgagcaaaat cagggtatca ctattattgt tgatgatgat ggcccagggg
      481 ttagcccaga agatcgcgag catattttcc gacctttcta tcgtacggac gaagcacgtg
      541 atagagaatc cggcggtaca gggctggggc tggcaatagt tgaaaccgca attagtcaac
      601 atcgaggcca tgtaaaagct gacgatagcc cattaggggg attaagatgg gagatttggt
      661 tgccgaaaac aaaataaata taagctatct aaaagtcacc tttagattta acatactaaa
      721 atactaaact ttaattaatc gttaaaatga ataaatactt aaaaattatc agaaataaat
      781 tgcgttataa gtactgtgat gtttttccaa aattgataac aaaatcaata tataaagaaa
      841 gaatgaataa aaaattagat ttgagacatc cgcaaacttt caatgaaaaa ttgcaatggc
      901 taaagctaaa tttatataga gataatccgc tggtaacaca atgtgcagat aaatatgcgg
      961 ctagagaata tgttaaagaa tgtgggtgtc aagaaatact caatgaaatc catcaggtat
     1021 gggaagaacc acatgaaatt aatttttccg aattaccaaa taaatttgta ctaaaatgca
     1081 atcatggggc aggttacaat attatttgta gagataaaaa tagtatatct cctgataaaa
     1141 taaaacaaaa actatcaact tggttaaatg aagattattg gcgtttatct gtagaatttg
     1201 tttataaaga tgtacctaaa aaaataattt gtgaaaagtt cattgaaaca aaaaataatg
     1261 aattgccata cgattacaaa ttattctgtt tccatggtaa accattattt gtaatgatat
     1321 gtacagaaag ggaatctcac catccaaaat tctattttgt taataaagaa tgggaactac
     1381 taccctatgg tattgattat aaaaatagta atgtttcaac tgtgttagag aaacctaaaa
     1441 attatcaaaa attatttgaa tatgcagaaa aactatctaa accatttcct tttgttagag
     1501 tagatttata tttaaacaac ggtattatcc attttggaga gttaacattt ataccatcag
     1561 caggaatgga taaagaacta aataatgaag aagttaaaaa cgttgatata atatttggta
     1621 actacctaaa attaaactaa ggggagcccc cttagtttat cgacttgatt atttttctat
     1681 taattaaata aataaatcta gattttaatt tcatataagg ataataaaaa cttttcttct
     1741 cacaacgctt aaataaagaa actttttttc tgatgatagc gatcttaagc tccttataaa
     1801 aataaggttt tgattgtata tttttaattc ttattttttc caacaaagaa ttacattttt
     1861 ttatattatc taatgtgaga tattcggagg aaaaattcaa atcatataaa actttttcat
     1921 tttcattaat actaaatatc tctaatgcgc taatttgtgt ttttatagca aatgaagttt
     1981 gtgtattaaa gtattttttt ctattttttt ctggatgggt tcgatatttt aataagggtt
     2041 cttctaaatt agcaaaaata tcttttttac ccaattttaa taagttaacc cataatccat
     2101 aatcctgagc atatttgtaa tcttctggat atgtgatttg attatcttcg ataaatttcg
     2161 ctctaaacat caccgttgga tgataaaaat ttgttccaaa aattaatccc gcagcaatgg
     2221 cattatgagt agttggcggt accataaact tactacgatc ttcataaatt tccattgatg
     2281 taccacaaac catgatattt ttatgcgcct ccataaactc taattgtttt tgaagtcggg
     2341 taggatatgc atagtcatca gcatccatac gagcaatata ctcaccagaa gagagtaaaa
     2401 aacctttatt caaagactta gctaatccaa tattagattc attagtatag actttaactc
     2461 gactatcttg ctcttgatat cgtctaagta tatctacaga tacatcagta gaaccatcat
     2521 caatgatgat aaattcaaaa tctttaactg tttgattaag aatagaattt atcgcttctt
     2581 ctaagtgtac tgcatcatta tatactgaca ttaatacact aattttggga tgatttttca
     2641 tttttactta agagttattc actataattg acattatata tactataaat taattaataa
     2701 aattagtgaa tatcaataag catttaaatt aattgaaata attataatca ttaagcataa
     2761 atagctatta cgttctagaa catcaaatat caatatttaa actgagtgct tattttctcc
     2821 ctaagaaaaa ataaaaacca tagaaatcag tcatatatat tatattagag ccaataatag
     2881 atcatccatc accacaatat ataaaatatt gtataatata taagcaagat atcaccgtta
     2941 cagagaatac ttacttacag ctaattcaaa taaacaacaa aattactgca ttagcaaact
     3001 atgcggttta tcaataaatt ggcggcatat tcttcttata cttatccttt atgcataatt
     3061 cagaatgatc ttgctaaatc agtcgatatt ccgatactta tatataccta atcaatgtta
     3121 gaatacccat caattacttg acccgctggt ttgaagagtt taatctcagt ggggtttgta
     3181 tactgttaaa gtatgcttat ataaaaaaac tcttatatta cagaaagata agagttttta
     3241 gagataaatt ttggcgctct gggagccgta aacctagtgc ggtagctata tattaaaaaa
     3301 aacaactatt atgaataaac ctaagcatcc tttatcacta agagtgatct actttttagc
     3361 aaaaagaaat tttctatcat ttttaagcga tagtttgtat ttgaaaatta ttttttattt
     3421 acacagagga gaaaaattag atctacaaaa tcctaaaact ttctcccaaa aaatacagtg
     3481 gctaaaagtt aatataaaag atccacaact acatattctt gttgataagt ttcttgtaag
     3541 agactatata aaaaataaaa taggatgcga tattctcata cctatgctag gaatttataa
     3601 caatgtggat gatataaatt ttaacgttct tccggatcaa ttcgttttga aaacgaccca
     3661 tggttctagc cagaatataa tatgtaaaga taaaaaaaac ttagatataa ataaaacaaa
     3721 agcgaaattt aaaaaatggg aaaaagtatg cctatataag atattcaaag aatgggcgta
     3781 taaagatgta acacctagaa ttatttgtga agaatttatt gggctaaata ataatgttcc
     3841 caatgattat aaattttatt gcttcaatgg aaaaatagga tttattcaag taaataaaga
     3901 aagatttagt aataatcata ctgaaacgtt ttttaatgaa aattgggaaa tgttagactt
     3961 taattatggt gtagagcaat ctacttcaat gattgaaaaa ccagaaaatt ttttagaaat
     4021 gaaggaaatc gttagagttt tagctaaaga acataagttt atcagagttg atttatataa
     4081 tgtaaataat aaaatttatt ttggagaatt aactttctat cctgcaagtg gtcttgaatt
     4141 tatgaaccct gaatctttag aaaggacttt cggtgatatg ttagatctgt catgaatatt
     4201 aaaggtaata ttatttatat gtatatagca tatttattta atatgctatt tcctttgatt
     4261 atagttccac tattaacaaa caatttatct cttgaggatt atggtaaata ctctttagtt
     4321 tatacttgtt tttcatttgc cattatttta tctgacttta gtttcaatat tacaggtatc
     4381 agatattatc taagagaaaa aaatgattat aataaaaatt ttatttatca aaagattcaa
     4441 tcttctaaaa agattatatc atacatatcg acaaccatac taacattata tattcattat
     4501 atatataatg atgtatatac ttcagtaata ttttttatat ctactctatt cggtttacat
     4561 ggctatataa actttagctc ttggtatttc caggccatag gtcagttgag aaataatacg
     4621 atatatctaa taataatgaa attcatatca ttaataataa ttgtaactct aattaaacta
     4681 aataataata attggataaa catatatgct tgttccacta ttctatatta cccttttggc
     4741 ataattatac aaagaaaatt aaaaaaaata aaaataaaaa taaaaataaa atttgttttt
     4801 aatttaatca aaagtaattc aggtttgttc cttgctgatt ttttacctaa tttatataat
     4861 ctaataccta tcattatatt aggcatgtat attgatgctt cagattatgc gatattcagc
     4921 cttagtaata gaatagcatt aattatttgt ggttttattt atgtcttttt aaaagcaata
     4981 tatccatact tctgtacaaa agatacaata aacactaata gatttatatt actcttagta
     5041 tttattactt taagtggata tttaataaat tatatttttg gtaaaaatat aataactttt
     5101 atttttggca atgactatat atatgcacaa gaatatttaa attatatatt cataggtata
     5161 gtttcaattt ctatttcaga aactatttta tactcgtact tacttcctaa aaataaagat
     5221 aattttatta ctaaaacgtc ccttataata gtaatactgt cagtagtgat tttactatta
     5281 acatttcacg catataaaca gtgggctgtt atttatacaa ttatctttgc aagagtcctt
     5341 tattctttaa tctacttatc atactattct ttttataaaa atgaaaaaaa ttaaaatagt
     5401 tatactaatg tctatctatc aaaatgacaa aaaaaaatgg gtttgtgata gtataaatag
     5461 catattaaac caatcagaat ctaattttca cttgttatta tctattgatg gttatattaa
     5521 tgacgaatta aagaaagaaa tcgatttttt taaaaaacat cccaagatta ctgtatttat
     5581 ttataatgaa aaaaaaggat tagcaacaag attaaatcaa atgattgatt ttgctatatc
     5641 taattttgaa tttgattatt ttgctcgaat ggatgcagat gacatatgca ccttagatag
     5701 attagaagta caatgtgact tcttatcaaa aaataaaaac attgatatta ttggatctag
     5761 tgtaatagaa atggatcaaa atgaaagacc attaaatata aaaaaaatgg aatccaatca
     5821 tgacatttta aaaaaaaata ttataagaaa atgcccatta aatcatccaa cagtaatgat
     5881 taaaaaagac gtatttatga acaaccatag atatgatcaa aatttaatga atacgcaaga
     5941 ttatatgctc tggataaact taatggctag tggatataaa ttttctaata tagaaaaacc
     6001 tcttttgtat tttagaatat ccaatagttt ttatagtaaa agaggaatta acaaagcagt
     6061 gaatgacttt accattagga tgattgcaat gaaaaaatta cattgttttt cattaaaaaa
     6121 ctttttattt accaatttaa tttttataat cagaatatct cctaaattta taaaaaaagt
     6181 agcatataaa tggggacgaa aatgaaaaaa gtaaaaagaa tttttttaga ggaaatatgg
     6241 tctttttacc agtggatctt tttatttatc ccaggcagag ttggacacta tacaagaggt
     6301 ttttttctca gttttttttt caaaaaacat gggaaaaaaa taacaattaa agagaatgta
     6361 gaaatatatc atcctgaaaa cttaataata ggctcaggtt ctggatttgg aagaaataat
     6421 gttattgatt gtatcggtgg aataactatt ggcaataatg ttagatttgg ccctaatgta
     6481 atgattgcaa ccatgaatca tgcccaaata ggtactagta taggttcagc gataaaagaa
     6541 aaaaaagcag tcaaaatcgg agataatact tggatcggta atggtgttac cattttacca
     6601 ggtgtagaaa taggtgataa ctgtatcatt gctgctggag ctgttgttac taaggcagtg
     6661 agatcaaata caagtgtagg tggagtacct gctaagaatc taaatttatg aaaataatct
     6721 gtcttttagg tgaaaagttt tataaagaaa ataataaata tcattatagc tcaagctcat
     6781 gtcttttcat aaatgaaaca ttttcagaag agaatgttac atttatgtct caagtaaaaa
     6841 aatacgataa tgaaaaaact gttggttctt tagagaagaa taaatttatt gaattacctt
     6901 tttttgattc caatatttca ctaattaaat atttatttaa tccaataaat tatatatatc
     6961 ttaataaaaa aatgaatttt gttatcaaca ataatccaga tgccattttt tgggtaagaa
     7021 atccggcatt gattagtatt atatttacaa gtaagctaat gaaaaaaaat aaatttttta
     7081 tatctcactt ttgcgccgat gtgaaaggaa tatcaaaaat aaaatataaa aaaggattta
     7141 aatatttcct atcctattta ttggaatcat atattttaaa taaaatggat aagataaatt
     7201 catatgaaaa atgtgttata ttctcaaccg gtgatatatt aaacaaaaga tataataact
     7261 ccactcattt attagatatt tccttaccaa agaaaacctc ctcgattaat acttatgaag
     7321 ataattactt tttatttgtt ggtagaattc aaaaagataa aggtattatt caattaattg
     7381 atgcatttaa cttatttaag ttgaacaata ataactataa attagttatt gtaggctatg
     7441 gaaaagactt tgatgaaatt aataatttaa ttaaaagtaa ctcctataat gatattatct
     7501 taactggccc caaaaacact atagaattac aacattttta ttcacattgc cattctctta
     7561 ttgttccatc ttgcaattcc tatgaaggat ttccaagagt tatagctgaa gcatggtatt
     7621 atggtaaacc agttatttta actaatgtag gaggtataaa tgcattagca ataaataatg
     7681 taaactcttt aattatagat gattcaactc ctagtaaaat taaaaatgca ctaataaaaa
     7741 tcactaataa agaattatat gaaaatataa aaaataatat aaaaaataat ataaaattat
     7801 ctgagagata ttattggaat aatctagtca gaaaaatagt aagtaatctc catgaaaaat
     7861 tttagtcttt tcctttctct atttctagcc acactggcaa atcacagtgc tataactatt
     7921 ccattagcat ttgtatttct tcttatttct ctttttttta ataaaggaaa atgctatttt
     7981 tcctcacaga aattaataat actaataact tctataactt ttataattat acattcaata
     8041 ttattcaaat taagcatgtt gaattacatt ccagcaaaag aatttattga ttcttttgaa
     8101 attaatttct ataaatatat tcttcttgct agttatgttt ttattatttc tataattata
     8161 caacatgaaa aaaatgaatt gctaattaaa atactaagtt ttattttaat tatccacatt
     8221 acttttttta taattcagtt ttttgttgtc tacttaaccg gttattattt ggattttatt
     8281 tatccaataa cacatgaaca ctctagatat ttattctatg gtgttgaaaa caatatttca
     8341 atatatcgat gtactggcct ttttgtagag ccatctacct attctatatc tatatttact
     8401 cttttattaa tattagaatc attaggaaat aaaaataaat ttttatttta tctaacatta
     8461 ctaacaatga ttcttagttt atcaagtatt agctttattc taattccttt ttattactta
     8521 ctaaaaataa taagaaaaat aaaactttct tacttaattg taacaatcct attattattt
     8581 tctttttcct atttttctca aaataatttt attaaaactc aaatcgataa gattaataat
     8641 acatctagca ttcgatttaa tcttgttaat gctatttttg atagaaaaat tgatttactt
     8701 ataacaggtt ccggtttata cggtattgaa aaaaacataa ttgatggaac caaagctact
     8761 tgtaatgata atgaaacttg ttcaaatgaa tcgataaata gaaagtatgc aacacctaat
     8821 gactatgggt tactatttta tatttttata aaattgggtt tttttagttt tattatatta
     8881 tatttaatat gttatccatt tataacgaat tataaaaaaa tgtctttttt tatttgtgtt
     8941 ctcattacta aattacaatt tgcatttcca ttattttgga tatttgtatt aatacttaga
     9001 agacaggatt caaatgaaaa tacttatcgt agttaaatgg ttaattaatg gtggcgctga
     9061 gaacaatatt attaggatag ctaactttct taataaaaat ggctttgata ttgatattct
     9121 tgtactaaac gatactgcag aaaaaccatt aataaaagta attaacaaag gaattaagaa
     9181 tatttttgtt atgaaaaaaa aatataattt tttaaccttt gttttgaaaa acaagaaaaa
     9241 atataaaatt attatttgtg gggatcatcg cattggtgtt tatttttcaa tatttaaata
     9301 tttttacctc gaaaagaaaa cgaaaataat ttgtaggtgt attaataatc tagatttatt
     9361 aattggttct aagaattttc tttataagaa attatttaac tattttttta aaaagattga
     9421 tattatcata tctcaatgta aagaaatgga gaaaaactta aaaaacgaat ggaaagtgaa
     9481 cgatgtaatc acaatatata accctgtaaa taaatcaaaa ataataaaat caacgataaa
     9541 taataaaaat ttcattttca ttggtaggtt tagtaagcaa aaaaaattag acttcatgct
     9601 taaggcattt aatctcgcct taagagaaaa taatgatata catctaacct tagttgggta
     9661 tagagaatgg atggcagaag ataagttggt aaaaaaagat ttagattcat ttattgttaa
     9721 caataatctt caaaataaag taaatattat taattattct aaaaatacat ttaactactt
     9781 acttaactct gatatttttt tactcacttc tgaatatgaa ggttttccta atgtactatt
     9841 agaagcaaac tcattaggtt tacctgtaat atctgttgac tgtccctttg gccccaaaga
     9901 aataataaat caagataatg ggttacttgt gaataaagaa tcatatatag aattttctaa
     9961 aaaaatcatt ttatctactc aaataaattg ggatcctata aaaatatcta attcaactat
    10021 tttatatgat gaaaacaaac agtattctaa acttataaat gttattaaag gattatctta
    10081 atgaaaatca ccatctccgg tactggctat gtcggtctat ctaatgggat acttctttcc
    10141 caaaacaaca aagttgttgc attagatatc aataaagaaa aagtcgaact attaaacaaa
    10201 aaaatctctc ctatctctga taaagaaatt gaagagtttt tagccacaaa agagctcgac
    10261 tttacggcaa ctttagataa agaattcgct tataaagatg ctgaattcgt tatcattgcc
    10321 actcctaccg attatgatcc taaaactaac tactttaaca cctcttctgt agagtcggtt
    10381 attcgtgatg ttttagaata taatccaaac accactatga ttataaaatc aaccattcca
    10441 gttggtttta ctaaacagat gagagagaaa tataataccg ataatattat cttctctcct
    10501 gagttcttac gtgaaggtcg cgccctttac gataatctct acccatctcg tattgtgatt
    10561 ggtgaacaat ctgcaagagc agagaaattt gctgagcttt tacaacaagg ggcattaaaa
    10621 aaagatattg atgttctttt tactgactct acagaagcag aagcaatcaa actatttgcc
    10681 aatacttatc tcgcactgcg tgttgcttat tttaatgaac tcgatagcta cgcccaagct
    10741 tacaatttaa atgcaagaca aattattgaa ggtgtctgtt tagatccacg tattggcaat
    10801 cactataaca acccctcatt tggttatggc ggttactgct taccaaaaga tacgaaacaa
    10861 ctgttagcca actacgaatc agtaccgaac aatattattg gtgccattgt ggaagctaac
    10921 cgcactcgta aagactttat tgctgattct attatcgcta aatcaccgaa gattgttggt
    10981 gtttatcgtt taattatgaa atcaggctct gataatttcc gctcctcttc aattcaaggc
    11041 attatgaagc gtattaaagc aaaaggtatt gaagtagtaa tttatgagcc agaaatgaaa
    11101 gaagagactt tctttaactc aaaattaatt cataatttag atgagtttaa agcaatgtct
    11161 gatgtcatta ttaccaatag aatggcttca gcactaaacg atgttgaaga aaaagtttat
    11221 actcgagacc tctttggtaa cgattaatta atagaaacgg attaattacg taatgaaata
    11281 tttagtcaca ggtgctgctg gatttattgg ttttcatcta ataaaaaaac tgatccagca
    11341 gggtgaaacc gttgttggta ttgataacct taatgattat tatgatgttg ctttaaaaga
    11401 agctagactt aatcttttaa atcaactgga taatttctct ttttctttta ttgatttagc
    11461 tgatagagaa aaaattgctc aattatttga gattgagaaa tttgataggg ttattcattt
    11521 agctgcacaa gctggtgtta gatattcact aattaatccg ttttcttacg ctgatagtaa
    11581 tttaactggt tttctaacta ttttagaagg ttgccgacac aataacgtta agcatcttgt
    11641 ttatgcatca tcaagttcag tctatggctt aaatgatgaa ttgccatttt ctcctcatga
    11701 tcaggctaat catcctgttt ctttatatgc agccactaaa aaagctaatg aattaatggc
    11761 gcatagctat tctcatttat atggtattcc gactactgga ttacgttttt tcacagtcta
    11821 tggcccttgg ggacgccccg atatggcatt atttaaattt actaaagcca ttattaacaa
    11881 tcagcctata gatatttata accatggtga aatgaaacgt gatttcactt atgttgaaga
    11941 tattgtagaa ggtgttacac gtattgctga tgtgatccct acagcccacc aagattggaa
    12001 agtaagtaca ggtaccccgg cagatagttc tgcaccctat aaggtttata atattggtaa
    12061 tggatctccg gtaaatttaa tggattacat taatgcatta gaaatacatt taggtaaaaa
    12121 agcagataaa aatatgttac caatgcaacc cggtgatgtt tacaccacat gggcagatac
    12181 tgaggattta tttaaagcaa ctggctataa acctcaaaca agcgttgatg aaggtgtaaa
    12241 acagttcgtt gattggtata aaaactatta tcaagtaaaa taaataatgc tcaatatcgt
    12301 cttatttgaa cccgaaattc caccgaatac gggtaatatt atccgcctgt gtgctaacac
    12361 aggctttcat cttcatttga tccaaccatt gggttttact tgggatgata aacggctacg
    12421 ccgtgcagga cttgattatc gtgagtttgc tgatatcaaa cagcatcacg attattatgc
    12481 ttttttagaa agtgaaggat taagccctga taatgcccaa tcgccaacag gggctaggtt
    12541 atttgcatta accactaaag gtactcccgc ccatagtaat gtaagttatc aagatgggga
    12601 ttatctgcta tttggccctg agacacgagg tttaccacct tatgtgctgg ataatatgcc
    12661 aacacaacaa aaaatccgtg tgccaatgtt agcggatagt cgcagtatga atctttctaa
    12721 tacggttgcc gtggttgttt ttgaggcatg gcgccaattg ggctaccctg gtgcgttatt
    12781 aagagattga tttagtggtt agtttggtta attaaagtta ttttaattca tactcattac
    12841 ttgattttat ctttatttat aaactaaaaa aggtgctaat atttattcag cacctttttt
    12901 ataaaccttt gttcgagcat tatacctgct tgatatccgc aatcactttc gtgatatacc
    12961 gacacccttc ccctacatcc tttaatacta atgcatgggc gccaataatc gcattatcac
    13021 caatcttaat ctcaccacca ataatggacg aattacagcc aatataaaca tggtttccta
    13081 cttggatatt ggcttttccc cctttctctt ttacaccaat agtcacccct tgcataatag
    13141 tgacatattc ccccagttgg cagtaattag ttagtacaat accaatcggg tgtggtaaag
    13201 aaaggccttt accaatacga gtttttaacg agatatcatt acagtatttg cggttattgc
    13261 tagcaccgat ccgaatacct aatttatgta aaaagcgatg gggagagaaa taaaaatact
    13321 gtgctacccg ccaccaaaat aggtaacggg aacgctgaca gaagaatacc cgatgaaata
    13381 atcttaacca actaaagggt tttttatcac caatatattc agcctgtaag caatctttta
    13441 gttcagcaag ggtaacctta gcttgggctt gcatagttaa ataccatcac cacactcaaa
    13501 gccgggtaca acaccattaa agtgctgatc catatcaaat gacggttttt cactatcagg
    13561 acgaccgaca atgcgagcag gcaccccggc cactgtagta tgagccggga cttcatgtaa
    13621 tactaccgag ccagcaccaa ttttggcacc acgaccaatc tcgatattgc caagaatttt
    13681 agcccccgcg ccaatcatta ccccttcacg cactttcggg tgacgatcgc cccctgtttt
    13741 acctgttccc cctaaggtca ccgattgtaa aattgagaca tcattttcaa ccaccgccgt
    13801 ttcaccaatc acaatacctg tcgcatggtc gagcataata ccgtgtccaa tacgtgccgc
    13861 cgggtgaata tccaccccaa aggtgactga tatttggttt tgtagataaa tggctagcgc
    13921 ttggcgattt tgttgccata accaatgagc aatacggtag gcttgtagtg catgaaagcc
    13981 ttttaaatag agcagtgggg tagaatattt atccaccgcc ggatcacgta ggcgtaccgc
    14041 ggagagatcg caagcggccg actcaatcat tgagctatcg tgacgataag cctcttcaac
    14101 gatttcgcgt actgcaatgg ctggcatagt ctgagttgcc agcttattgg ccagcatata
    14161 gcttaatgca ctacctaaat tttcgtgttt gagaagtgtc gcatgaaaga aactggctaa
    14221 aattggctca caatctgcca atgctcgcgc ttcgctttta atatgatccc agactcttgt
    14281 tagttcttct agcgacatac tcactctcat tttttacacg aaagcctaag taaatactta
    14341 ggctttgaat tttcaaacaa atggatagtt aaaacagata ccctaagaat attatttaaa
    14401 ataaacttac aataggctgc tgtctatctc atctttggtt gcccgaccca ataaagccaa
    14461 tgcagcctct tttacatctt tatgttgata taaaatctgg tagatctgtt cggtgattgg
    14521 catttctaca cccacttgtt cggctaatgc gcgaacttct ttggtgttac gataaccttc
    14581 aactacctgc ccgattttct cttgggctgt atcaacatca aaaccttgac ctagcatcat
    14641 accaaagcga cgattacgtg attggttgtc agtacaggtt aaaactaaat cacccaaacc
    14701 agccatgccc ataaaagttg ccgcatctgc acctaaggct ttacctaacc ggctcatttc
    14761 ggccagacca cgggtaataa gtgcagtacg cgcattagcg ccaaacccca taccatcaga
    14821 catacccgca ccaatagcaa tcacattttt aacagcgcca cccagttgca caccgataaa
    14881 atcagggttt ttatagactc ggaagctttt accacaatga aatagctgtt gtaactgttc
    14941 taaaaagaga ttgtctgtcg aggccactgc aatcgcggtg ggcaaacccg cggcaagctc
    15001 tttagcaaaa gtaggaccag ataacactgc gagcgggatt tcattaccta atacttcacg
    15061 ggcaacatct tgtaataagc gaccagtatg tgcttcaaga ccttttgtcg cccaaacaac
    15121 acgtgcatcc tgacgtaaaa agggtttgat ttgttgcagt acttcaccaa acacatggct
    15181 tgggatcaca acgagaatat tacggctcgc ttcaatcgct tttttgcaaa gaagcttcca
    15241 tatatcaact atcaggaaag gaacatcggg cagaaaggct tgattacagc gcgcttgttc
    15301 taatgccgca acgtgcttag gatcatgccc ccacagcaca acatcatggc cattacgcgc
    15361 taaagtaatc gctaaagcgg tgccgtatga accggcaccg ataactgtca tagaagcgtt
    15421 cattaagctt cttcaactcc tgagtttgct gcatcctgtt gctgttgttg ctgtaaatag
    15481 ttcataaaca acgcatcaaa gttaacaggt gctaagttta attgtgggaa agtaccacga
    15541 cccactaagt tggtgataca ttcacgtgca taagggaata gtacgttc
//
