LOCUS       GU254062               13935 bp    DNA     linear   BCT 14-AUG-2010
DEFINITION  Proteus mirabilis strain Prk 50/57 O antigen gene cluster, complete
            sequence; and WemX (wemX), CysE (cysE), and GpsA (gpsA) genes,
            complete cds.
ACCESSION   GU254062
VERSION     GU254062.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 13935)
  AUTHORS   Wang,Q., Torzewska,A., Ruan,X., Wang,X., Rozalski,A., Shao,Z.,
            Guo,X., Zhou,H., Feng,L. and Wang,L.
  TITLE     Molecular and genetic analyses of the putative Proteus O antigen
            gene locus
  JOURNAL   Appl. Environ. Microbiol. 76 (16), 5471-5478 (2010)
   PUBMED   20581173
REFERENCE   2  (bases 1 to 13935)
  AUTHORS   Wang,Q., Torzewska,A., Ruan,X., Rozalski,A., Shao,Z., Guo,X.,
            Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..13935
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="Prk 50/57"
                     /db_xref="taxon:584"
                     /note="serogroup: O27"
     gene            887..2143
                     /gene="wzx"
     CDS             887..2143
                     /gene="wzx"
                     /note="O unit flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ADL32319.1"
                     /translation="MTIFKTASIYVLSNIINAAIPFLLLPVITKYLSQEEYGQAAMFQ
                     SLVLGLAAFVGLNIQGFSIRKYFDKNDVINTEYLANSNGACFLVLIFTTIITSIFLGI
                     FNKEISNLLSIPSEWINIAVLTSATSFIVQLRLGQWQVRNDAIKYGLLLITQSSLYMG
                     LSLIFIISMKLGSEGRILGQFYSTLIFSIISIGYLYKDKLLKLDNIKYSMLKEIIFYG
                     IPLIPHTFGFFIIYTSDRIILNKYLGLSSVGIYVLAVQVSSVVTILCDSINKAYMPWL
                     FSKLKLNNKNVNLSIVKSTYYYFLFLIFMSFLSFLIGPYIISFIAPTYIEASHIIGYL
                     CLGQAISSMYTTISNYLFYTKNTGVISTITIISGILNAILMAIMIYYSGLFGAALAFC
                     ISKLIQLIMVWYISNKYYPMPWFIYR"
     misc_feature    887..11219
                     /note="O antigen gene cluster"
     gene            2151..3242
                     /gene="wzy"
     CDS             2151..3242
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ADL32320.1"
                     /translation="MQIIILYYTLIAIFIIIVEANYKNLKLSKNSLYFIYSTPLLIFF
                     SFTASRDYNIGTDTENYLYFFSKVIDSDNLSIFSTSRFEFLFIFISYIASFLENERFY
                     LIIVSFIQTIAFFFAIRLWSKNPVVSFLFFCSLFVSYNLGTNILRQGLAIPFALISIH
                     YLLEKKIRPFIIFLLISFFFHHTSIIMILFSFLCLNKFRIRTYIIFWLIAIILLYSGS
                     LKFILELGRDLFSFNESYRNILSNFSLDSYQVGFRLDFILFSSIPLLILLITNKKNII
                     KENIFLIKLYFSLNTFFILIMEIPYSDRFGLYSWILFPLLIPNLIKENKFKILRNNVI
                     WLFLTFIAGVVSFVFYPIMQVSLNFNELI"
     gene            3251..4228
                     /gene="wemQ"
     CDS             3251..4228
                     /gene="wemQ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32321.1"
                     /translation="MEKFSIVIPMYNVEKYIHQCIQSILSQTYQNFEVIVIDDGSSDN
                     SSKIVDEIIKSEKRVTLIKNKNNGPSISRNIGIEKSTGDIILFLDSDDMYVPNTLQTC
                     LDEINKNRLDILYFNSRGFCDEFNEIQNKQALDFIGDRKRHINIINKVLTGKQYYSLM
                     FKYNNFYSSICYCAFRSSIIKNQRFVDNVIHEDELFTAEILLKQNTKRVLCIPDILYL
                     RRIRQNSIMTTAIQEKNITSYRIIIEKLLLLNVKNIYLETHINKLAFNKAYYRLKLKN
                     KITLNERLDNMKWVYNLNVAKKKNKIILYMLSLFPEITNTYQKMKKIIK"
     gene            4225..5160
                     /gene="wemR"
     CDS             4225..5160
                     /gene="wemR"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32322.1"
                     /translation="MKKLKKYLTRKKKENYIIFSIYYFIKVTSSIFISDSLYRKYIFK
                     RKYKRKLNLKKPTSFNEKIHYRILNDHNPIYTKLADKLLVRDYVREKIGEKYLIKLIN
                     HYNTPSEINFNTLPKSFVLKCNHDVGSVMIINDKSKINEKAIKKKLKIALKNNIYYQN
                     REWHYKNIKPKIICEELINIFPHNKKNYPEDYKIHCFNGIPRYIELQFSRFSHDRRIN
                     IYDFNWNLQPFLMGYKNTNESIEKPKKLQEIYNISKTLSADFDYCRVDFYITPDDSIY
                     FGELTFTPCNGMDDFYPNEWDYLFGKEWIIDDSRK"
     gene            5144..5677
                     /gene="wemS"
     CDS             5144..5677
                     /gene="wemS"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32323.1"
                     /translation="MIQENKNAYPLVSIIISTYNRKNLLKRAITSAIKQDYPNIEIII
                     SDDNSLDDNEEMIENIKKQTTIPIFYMKNKKNMGACFTRNQGIKIASGKYIAGLDDDD
                     EFTPNRISLLVKIYNISKTLSADFDYCRVDFYITPDDSIYFGELIFTPCNGMDDFYPN
                     EWDYLLGKEWIIDDSRK"
     gene            5661..6509
                     /gene="wemT"
     CDS             5661..6509
                     /gene="wemT"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32324.1"
                     /translation="MIQENKNAYPLVSIIISTYNRKNLLKRAITSAIKQDYPNIEIII
                     SDDNSLDDNEEMIENIKKQTTILIFYIKNKKNMGACFTRNQGIKIASGKYIAGLDDDD
                     EFTPNRISLLVKNYNPKYSLTTSNSILITKNKKYNLFKDKKAKIISFKDSLWENQVGT
                     QCLVESERIRNCNGFDTNLTSAQDADMWIRLIQKYGNALRLPQVTYLIHTEHENNRIS
                     TSAKKITGLITYYKKHEPHMSSGQKSYMNFKIHYWKNKKITFKSLFLLTPSSIFHILS
                     VFFKRK"
     gene            6506..7384
                     /gene="wemU"
     CDS             6506..7384
                     /gene="wemU"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32325.1"
                     /translation="MKKIKYFIRDVLRILLGRYLYTYIRFLFVHKYIPNLNKPSSFSE
                     KIIFRKLYDNPKKYSSFVDKYTVRKYIESKVGSDYLIPLLKAKKFITPSDFDSLPNEF
                     VIKTSNGGGGENVLIVNNKNSLNLDEICDRFNNYLKVKIGKKIDEYFYDIEQPMILFE
                     KLLRDDFYGYPPDFKIHIFNGIKNKKIIQIDEGRFNHHKRSLYNEKKEKLPFNIQPKY
                     ESVSDNFPWPKNWNEMLDISMKLADDFKYVRVDLYNVNGKIFFGELTFCHGSGWEPIY
                     PKKYDFLLGKYWHEYE"
     gene            7377..8495
                     /gene="wemV"
     CDS             7377..8495
                     /gene="wemV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32326.1"
                     /translation="MNNKKIAITSNTSWYLYNFRKNTIISLIEAGFKVYTVSPIDSYS
                     NKLKELGAEHINIKIDSASKNPLIDLYTLISFYKIFKQLRCNAVLNFTPKNNIYGTLA
                     AKLNKVKVINNIAGLGTLFVNDTISCKLARNLYRYSQKYADKIFFQNNDDLSLFLEKK
                     YVDKVQVDRLPGSGVDLSRFTLSLSEYKHKFRFLLVARMLYEKGITFYVEAARILKER
                     YGDKVEFCLLGFVGVNNPSAITHKQMNNWVAEGIINYLGTSDTVEHELAQADCIVLPS
                     FYREGVPKTLLEAGAMGKPIITTDNVGCRETVTHGFNGYICQPKSVSSLVDAMDRFIN
                     LPYEKKLKMGKNSRQKIETEFDERIVIKKYLDALKEIM"
     gene            8510..9676
                     /gene="ugd"
     CDS             8510..9676
                     /gene="ugd"
                     /note="UDP-Glc-6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="Ugd"
                     /protein_id="ADL32327.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYNLNARQIIEGVCLDPRIGDHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIHNLDEFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     gene            9702..10712
                     /gene="gla"
     CDS             9702..10712
                     /gene="gla"
                     /note="nucleotide sugar epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gla"
                     /protein_id="ADL32328.1"
                     /translation="MKYLVTGAAGFIGFHLIKKLIQQGETVVGIDNLNDYYDVALKEA
                     RLNLLNQLDNFSFSFIDLADREKIAQLFEAEKFDRVIHLAAQAGVRYSLINPFSYADS
                     NLTGFLTILEGCRHNNVKHLVYASSSSVYGLNDELPFSPHDQANHPVSLYAATKKANE
                     LMAHSYSHLYGIPTTGLRFFTVYGPWGRPDMALFKFTKAIINNQPIDIYNHGEMKRDF
                     TYVEDIVEGVTRIADVIPTAQQDWKVSTGTPADSSAPYKVYNIGNGSPVNLMDYISAL
                     EIHLGKKADKNMLPMQPGDVYTTWADTEDLFKATGYKPQTSVDEGVKQFVDWYKNYYQ
                     VK"
     gene            10716..11219
                     /gene="wemW"
     CDS             10716..11219
                     /gene="wemW"
                     /note="methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WemW"
                     /protein_id="ADL32329.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNVQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     gene            complement(11349..11903)
                     /gene="wemX"
     CDS             complement(11349..11903)
                     /gene="wemX"
                     /note="serine acetlyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WemX"
                     /protein_id="ADL32330.1"
                     /translation="MHAQAKVTLAELKDCLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGDHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGERCRYITKVIADIRQV"
     gene            complement(11906..12739)
                     /gene="cysE"
     CDS             complement(11906..12739)
                     /gene="cysE"
                     /note="serine acetlyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="CysE"
                     /protein_id="ADL32331.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPMLASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     gene            complement(12839..13852)
                     /gene="gpsA"
     CDS             complement(12839..13852)
                     /gene="gpsA"
                     /note="glycerol-3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="GpsA"
                     /protein_id="ADL32332.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
BASE COUNT     4947 a   2208 c   2213 g   4567 t
ORIGIN
        1 gagttattac gtgaaaccgt aaaagcgaat gagctttgga acgatatttt agataatgca
       61 aaatttgagg cagaacaaag caataaaacc ctaacagtaa ccgccccacc aggcccatgg
      121 acaatttatt gcaatcctta ttcattagcg agtgcctttg aaaatatcgt tcgcaatgcc
      181 ttgcgttatt cacattctcg tattgaagtg gcctttaccg aacaaaatca gggtatcact
      241 attattgttg atgatgatgg cccaggggtt agcccagaag atcgcgagca tattttccga
      301 cctttttatc gtacagacga agcacgtgat agagaatccg gcggtacagg gctgggactg
      361 gcaattgttg aaaccgcgat tagtcaacat cgaggccatg taaaagccga tgatagccca
      421 ttagggggat taagagtgga gatttggttg ccgagaggga gtgtggtaaa taaatcacaa
      481 cacgcttaat tcagtaataa aattactata ttaaattatt atgccatcta ttgaagaata
      541 ggtggcatat tcttttcatt cccatcttta tagatatttc agaatgatct tgctaattca
      601 gtcgatattc cgatacttat atatacctaa tcaatgttag aatacccatc aattacttga
      661 cccgcgggtt tgaagagtct aatctcagag cggttttgcg tactgttaaa gtatgcttat
      721 ataacaaaac tcttatatta cagtaagata agagttggtc gggaaaaatt tggcgctctg
      781 ggggccgtaa acccagtgcg gtagctatgg ctactaaaca aaaaattatt ttgataaaaa
      841 tttcttcttt atttaaaaga aaacacacaa tgaattacct ttttatatga caatttttaa
      901 aaccgcatct atatatgttt tatcgaatat tattaatgct gcaatcccat ttttattatt
      961 accagtaata acaaaatact taagtcaaga agaatatggg caagcagcta tgtttcaaag
     1021 cttggtatta ggtttagcag cttttgtagg attaaatata caaggttttt ctattagaaa
     1081 atatttcgat aaaaatgatg tgattaatac tgaatatttg gctaactcta acggtgcttg
     1141 ctttctagtt cttatattca caacgatcat cacaagtatt tttttaggaa tatttaataa
     1201 agaaattagt aacctattat ctatacctag tgaatggata aacattgcag tattaacatc
     1261 agctactagc tttattgttc aattaagatt gggacaatgg caagtaagga acgatgctat
     1321 aaagtatggc ttattattaa taacccaaag ctcattatat atggggcttt cacttatatt
     1381 cataatttca atgaagttag gatccgaagg tcggatttta ggtcaatttt attcaaccct
     1441 aatattctca ataatatcaa ttggttactt atataaggat aaattattaa aactagataa
     1501 cataaaatat tcgatgctta aagaaataat attttatggc attccattaa tacctcatac
     1561 ttttggcttt ttcataattt atacatctga tagaataata ttaaacaaat acttaggttt
     1621 atcatctgtc ggaatttatg tattagcagt acaagtcagc tctgtagtta ctatattatg
     1681 tgattctata aataaagctt atatgccttg gctttttagt aagctaaaac ttaataataa
     1741 aaacgttaat cttagtattg taaaatccac atattattat tttttatttt taattttcat
     1801 gtcctttcta tcctttttga taggtccata tattatatca tttattgctc ctacatatat
     1861 cgaagcaagt catattatag gatatttatg cttgggccaa gctataagtt ctatgtatac
     1921 gacgatatca aactatcttt tttacactaa aaatacaggt gttatttcga caataacaat
     1981 aatatcaggt attttaaatg ctatacttat ggctataatg atttattact ctggtttatt
     2041 tggtgctgca ttagcttttt gtatatcaaa actgatccaa ttaataatgg tttggtacat
     2101 ttcaaataag tattatccta tgccatggtt tatttataga tgaatttcat atgcaaataa
     2161 ttattttata ttatacattg atagctatat ttataataat agttgaagct aattataaaa
     2221 atttaaagct ctccaagaat agtttatatt tcatatattc aacacccttg ttaatatttt
     2281 tctcattcac agcttctaga gattataata ttggtactga cacagaaaat tatctttatt
     2341 ttttctcaaa ggtaatagat agtgataacc tttctatatt ttcaacatca agatttgaat
     2401 ttctttttat atttatatca tatatagctt catttttaga aaatgaacgg ttttatttaa
     2461 ttatagtgag ctttattcaa acaattgcat tcttttttgc tataagatta tggtcaaaaa
     2521 accctgtagt atcatttcta ttcttctgca gtttatttgt ttcttataat cttggaacaa
     2581 atatactaag gcaaggtcta gcaataccat tcgcccttat cagcatccat tatttactag
     2641 aaaaaaaaat taggccattt ataatttttt tacttatttc ttttttcttt catcatacgt
     2701 caataattat gattctattt tcattcttat gcttaaataa gtttaggata aggacttata
     2761 ttattttttg gctaattgca attatattac tatactcagg aagccttaag tttattttag
     2821 agttaggaag agacttgttt tcttttaatg aatcttatag aaatattctt agtaactttt
     2881 cattagatag ctatcaagtt ggatttaggt tagattttat attatttagt tcaataccac
     2941 ttttaatact actaattaca aataaaaaaa atataataaa ggaaaatatt tttttaatta
     3001 aattatactt ttccttaaat actttcttta tacttataat ggaaatacct tattctgata
     3061 gattcgggct ttattcgtgg atacttttcc cattgttaat accaaatctc attaaagaaa
     3121 ataaatttaa aatactaaga aataatgtca tatggttatt tttaactttt attgctggtg
     3181 ttgtatcatt tgttttctac cctataatgc aagtgtcatt aaattttaat gaattaatat
     3241 aaggtttaag atggaaaaat ttagcattgt catccccatg tataatgtag aaaaatatat
     3301 acatcagtgt attcaatcta tattatctca aacatatcaa aactttgaag ttattgttat
     3361 tgatgatggt tcatctgata atagttcaaa aatagtagat gaaattatca aaagtgaaaa
     3421 aagagtaaca ttaattaaaa ataaaaataa tggcccatca atttcaagaa atattggtat
     3481 tgaaaaaagc actggtgaca taatattatt cttagatagt gatgatatgt atgtgccaaa
     3541 tactctacaa acttgtctag atgaaataaa taaaaataga ctcgatatac tttattttaa
     3601 tagtagagga ttctgtgatg aattcaatga gattcagaac aaacaagcat tagattttat
     3661 aggtgacagg aaaagacata taaatataat aaataaggtt cttacaggta aacaatacta
     3721 ctctttaatg tttaaatata ataattttta ctcttctatt tgttactgtg catttaggtc
     3781 atcaataatt aaaaatcaaa gattcgttga taatgttatt cacgaggatg aattattcac
     3841 agccgaaata ttattaaaac agaatacaaa acgagtatta tgtattcctg acattcttta
     3901 cttaaggaga attagacaga attccattat gacaactgct attcaagaaa aaaatataac
     3961 tagctataga attataatag aaaaattatt attattaaat gtaaagaata tttatttaga
     4021 aactcatata aataaattag cctttaacaa ggcatattat agattaaaac taaaaaacaa
     4081 aataacatta aatgaaagat tagataatat gaagtgggta tacaacctaa atgtagccaa
     4141 gaaaaaaaat aaaataatat tatatatgtt atcactattc ccagaaatca ccaatacata
     4201 tcaaaaaatg aaaaagataa taaaatgaaa aaattgaaaa aatatttaac aagaaaaaag
     4261 aaagagaatt atataatatt ttcaatatat tatttcataa aagttactag ctcaatattt
     4321 attagtgatt ctttatatag gaagtatata tttaaaagaa aatataaaag aaagttaaac
     4381 ttaaaaaaac caacaagctt taatgaaaaa attcattata gaattttaaa cgaccacaat
     4441 cccatatata caaaattagc tgataaacta ttagtaagag attatgttag ggagaaaatt
     4501 ggtgaaaaat atttaataaa actaattaat cattataata ctccttctga aataaatttt
     4561 aatactcttc cgaaatcatt tgttttgaaa tgcaatcatg atgtaggaag tgttatgatt
     4621 ataaatgata agagtaaaat aaacgaaaaa gctattaaaa aaaaattaaa aattgcatta
     4681 aaaaataata tttattatca aaatagagag tggcattata aaaatataaa accaaagatt
     4741 atatgtgaag agctcattaa tatatttcct cataataaaa aaaattatcc tgaagattat
     4801 aaaatccact gttttaatgg cattcctcgg tatatagaat tacaattttc gcgttttagt
     4861 catgatagga gaattaatat atatgatttt aactggaact tacaaccatt tttaatgggg
     4921 tataaaaata ctaatgaaag tatcgagaaa cctaaaaaac tccaagaaat ttataatata
     4981 agtaaaactt tatcagctga tttcgactat tgtcgtgtag atttctatat tacaccagat
     5041 gatagtattt attttggaga gttaacattt acaccatgta atggcatgga tgatttttat
     5101 cctaatgaat gggattattt atttgggaag gaatggatta tagatgattc aagaaaataa
     5161 aaacgcttat cctttagtta gtattataat atcaacttat aataggaaaa atctattaaa
     5221 acgagctata acttcagcta tcaagcaaga ttaccctaat attgagataa taattagtga
     5281 tgataattca ctcgatgata atgaagaaat gatagaaaat ataaagaagc aaacaactat
     5341 acctatattt tatatgaaaa ataaaaaaaa catgggggct tgttttacta gaaatcaagg
     5401 aataaaaata gcttcaggaa aatatattgc tggcctagat gatgatgatg aattcacacc
     5461 taatagaatt agtttattag taaaaattta taatataagt aaaactttat cagctgattt
     5521 cgactattgt cgtgtagatt tctatattac accagatgat agtatttatt ttggagagtt
     5581 aatatttaca ccatgtaatg gcatggatga tttttatcct aatgaatggg attatttatt
     5641 agggaaggaa tggatcatag atgattcaag aaaataaaaa cgcttatcct ttagttagta
     5701 ttataatatc aacttataat aggaaaaatc tattaaaacg agctataacc tcagctatca
     5761 agcaagatta ccctaatatt gagataataa ttagtgatga taattcactc gatgataatg
     5821 aagaaatgat agaaaatata aagaagcaaa caactatact tatattttat ataaaaaata
     5881 aaaaaaacat gggggcttgt tttactagaa atcaaggaat aaaaatagct tcaggaaaat
     5941 atattgctgg cctagatgat gatgatgaat tcacacctaa tagaattagt ttattagtaa
     6001 aaaactataa tcctaaatac tctttaacta caagcaattc tattttaata acgaaaaata
     6061 aaaaatataa tttattcaaa gataaaaaag ctaaaataat ctcatttaaa gatagtttat
     6121 gggaaaatca agtcggtact caatgtttag ttgaatctga acgcatacga aattgtaatg
     6181 ggtttgatac taatcttact tcagcacaag atgctgatat gtggattaga cttattcaaa
     6241 aatatggtaa tgcgcttaga ttaccacaag taacttattt aattcataca gaacacgaaa
     6301 acaatagaat ttcaacttcg gctaaaaaaa taacggggtt aattacttat tataaaaaac
     6361 atgaaccaca tatgtcttct ggacaaaaat cttatatgaa ttttaaaatt cattactgga
     6421 aaaataaaaa aataactttt aaaagtctct ttttattaac tccatcatcc atctttcata
     6481 ttttaagtgt tttttttaaa agaaaatgaa aaaaataaaa tattttatcc gagatgtgct
     6541 tagaatactt ctaggcagat atttatatac ctatatcaga tttttatttg tacataaata
     6601 tattccaaac ttaaataaac catcatcttt ttctgaaaag ataatattta gaaaattata
     6661 tgataaccca aaaaaatatt cctcttttgt agataaatat acggttagaa aatatataga
     6721 aagtaaagtt ggatctgatt atttaattcc attactaaaa gcaaaaaaat ttataactcc
     6781 ttcagacttt gattcattac ccaatgagtt cgtaattaaa acaagcaatg ggggaggagg
     6841 agaaaatgta ctaatagtta ataataaaaa tagcctaaac ttagatgaaa tatgtgacag
     6901 atttaataat tatcttaaag taaaaattgg aaaaaaaata gatgaatact tttatgatat
     6961 agaacaacct atgatattgt ttgaaaaatt attacgagat gatttttatg gttatcctcc
     7021 cgattttaaa attcatatat ttaatggtat aaagaataaa aaaataattc aaattgatga
     7081 aggtagattt aaccatcata aaagaagctt atataacgaa aaaaaagaaa agcttccttt
     7141 taatatacag cctaaatatg aatcagtatc tgataatttt ccatggccaa agaattggaa
     7201 tgaaatgtta gatatatcta tgaaattagc tgatgacttt aaatatgtcc gagtagatct
     7261 ttataatgtt aatggtaaaa tattcttcgg tgaattaaca ttctgccatg gtagtggttg
     7321 ggaacctata tatcctaaga aatatgattt tttattaggt aagtattggc atgaatatga
     7381 ataacaaaaa aatagcaata acatcaaata caagttggta cttatataac tttagaaaaa
     7441 ataccataat atctttaata gaagctggat ttaaagttta tacagtttca cctattgata
     7501 gttactctaa caaactaaaa gaattaggtg cagaacatat aaatatcaaa attgattcag
     7561 ctagtaaaaa tcctttgatt gacctctata cattgatttc attttataaa atatttaaac
     7621 aacttagatg taatgctgta cttaatttta ctcctaaaaa taatatttat ggaacattag
     7681 ccgcaaaatt aaacaaagta aaagtcatca ataatattgc aggtttaggt acattatttg
     7741 taaatgatac tattagttgt aaactagcaa gaaacttata ccgctatagc caaaaatatg
     7801 ctgataaaat attttttcaa aataacgatg atctttcatt atttttagaa aaaaaatatg
     7861 tcgataaagt acaagttgat agattgcctg gttcaggtgt tgatttatct cgctttacat
     7921 tatcattatc agaatataaa cataaattta gatttctatt agtggcaaga atgctttatg
     7981 aaaaagggat tactttttat gttgaagctg cacgtatctt aaaagaaaga tacggcgata
     8041 aagttgaatt ttgtctttta ggatttgttg gtgtaaataa tccttcagca atcacacata
     8101 aacaaatgaa taactgggta gcagagggaa ttattaatta tttaggtaca tctgataccg
     8161 tcgaacatga attagcccaa gcagattgta tagttctacc ttctttttac cgagaaggag
     8221 tgccaaaaac attactagaa gctggagcta tgggaaaacc cataatcact acagataatg
     8281 ttggttgtcg agaaacagtt actcatggtt ttaatggata tatatgtcaa cctaagtctg
     8341 tatcaagctt agttgatgcc atggatagat ttattaactt accctatgaa aaaaaattaa
     8401 aaatgggtaa aaatagtagg cagaaaatcg aaactgaatt tgatgaaaga atagtcataa
     8461 aaaaatattt agatgcgtta aaagaaatta tgtaacttta ggataaaaaa tgaaaatcac
     8521 catctccggt accggctatg tcggtctatc taatgggata ctcctttccc aaaacaacaa
     8581 agttgttgca ttagatatca ataaagaaaa agtcgaacta ttaaacaaaa aaatctctcc
     8641 tatctctgat aaagaaattg aagagttttt agccactaaa gagctcgact ttacggcaac
     8701 tttagataaa gaattcgctt ataaagatgc tgaatttgtt atcattgcca ctcctaccga
     8761 ttatgatcct aaaaccaact actttaacac ctcttctgta gagtcggtta ttcgtgatgt
     8821 tttagaatat aatccaaaca ctactatgat tataaaatca accattccag ttggttttac
     8881 taaacagatg agagagaaat atcataccga taatattatc ttctctcctg agttcttacg
     8941 tgaaggtcgc gccctttacg ataacctcta tccgtctcgt attgtgattg gtgagcaatc
     9001 tgcaagagca gagaaatttg ctgagctttt acaacaaggg gcattaaaaa aagatattga
     9061 tgttcttttt actgattcta cagaagcaga agccattaag ttatttgcta atacttatct
     9121 cgcactacgt gtggcttatt ttaatgaact cgatagctac gcccaagctt acaatttaaa
     9181 tgcaagacaa attattgaag gtgtttgttt agatccgcgt attggtgatc actataacaa
     9241 cccctcattt ggttatggtg gctactgctt accaaaagat actaaacaac tattagccaa
     9301 ctacgaatca gtgcctaata atattattgg cgccattgtg gaagctaacc gcacccgtaa
     9361 agactttatt gctgattcta ttatcgctaa atcaccgaag attgttggtg tttaccgttt
     9421 aattatgaaa tcaggctctg ataatttccg ctcctcttca attcaaggca ttatgaagcg
     9481 tattaaagcg aaaggcattg aagtggtaat ttatgagcca gaaatgaaag aagagacttt
     9541 ctttaactca aaactaattc ataatttaga tgagtttaaa gcaatgtctg atgtcatcat
     9601 taccaataga atggcttcag cactaaacga tgttgaagaa aaagtttata ctcgagacct
     9661 ctttggtaac gattaattaa tataaacgga ttaattacgt aatgaaatat ttagtcacag
     9721 gtgctgctgg atttattggt tttcatctaa taaaaaaact gatccagcag ggtgaaaccg
     9781 ttgttggtat tgataacctt aatgattatt atgatgttgc tttaaaagaa gctagactta
     9841 atcttttaaa tcaactagat aatttctctt tttcttttat tgatttagct gatagagaaa
     9901 aaattgctca attatttgag gctgagaaat ttgatagggt tattcattta gctgcacaag
     9961 ctggtgttag atattcacta attaatccgt tttcttatgc tgatagtaat ttaactggtt
    10021 ttctaactat tttagaaggt tgccgacaca ataacgttaa gcatcttgtt tatgcatcat
    10081 caagttcagt ctatggctta aatgatgaat tgccattttc tcctcatgat caggctaatc
    10141 atcctgtttc tttatatgca gccactaaaa aagctaatga attaatggcg catagctatt
    10201 ctcatttata tggtattccg actactggat tacgtttttt cacagtctat ggcccttggg
    10261 gacgccccga tatggcatta tttaaattta ctaaagccat tattaacaat cagcctatag
    10321 atatttataa ccatggtgaa atgaaacgtg atttcactta tgttgaagat attgtagaag
    10381 gtgttacacg tattgctgat gtgatcccta cagcccagca agattggaaa gtaagtacag
    10441 gtactccagc agatagttct gcaccctata aggtttataa tattggtaat ggatctccgg
    10501 taaatttaat ggattacatt agtgcattag aaatacattt aggtaaaaaa gcagataaaa
    10561 atatgttacc aatgcaaccc ggtgatgtgt acaccacatg ggcagatact gaggatttat
    10621 ttaaagcaac tggctataaa cctcaaacaa gcgttgatga aggtgtaaaa cagttcgttg
    10681 attggtataa aaactattat caagtaaaat aaataatgct caatatcgtc ttatttgaac
    10741 ccgaaattcc accgaatacg ggtaatatta ttcgcctgtg tgccaacaca ggctttcatc
    10801 ttcatttgat ccaaccattg ggttttactt gggatgataa acggctacgt cgtgcagggc
    10861 ttgattatcg tgagtttgct gatatcaaac agcaccacga ttattatgct tttttagaaa
    10921 gtgaaggatt aagccctgat aatgtacaat caccgacagg tgcgcggttg tttgcattaa
    10981 ccactaaagg tactcccgcc catagtaatg taagttatca agatggggat tatctgctat
    11041 ttggccctga gacacgaggt ttaccacctt atgtgttgga taatatgcca acacaacaaa
    11101 aaatccgtgt gccaatgtta gcggatagtc gtagtatgaa tctttctaat acggttgctg
    11161 tggttgtttt tgaggcatgg cgccaattag gctatccagg tgcgttatta agagattaat
    11221 ttagtggtta gtttgaataa tgaaagttac tttcattcat actcattact tgattttatc
    11281 tttatctata agctaaaaaa ggtgctaata attatttagc acctttctca taagcctttg
    11341 ttctgcattt atacctgcct gatatccgca atcactttcg tgatataccg acacctttcc
    11401 cccacatcct ttaatactaa tgcatgggcg ccaataatcg cattatcacc aatattaatc
    11461 tcaccaccaa taatggatga attacagcca atataaacat gatctcctac ttggatattg
    11521 gcttttcccc ctttctcttt tacgccaata gtcacccctt gcataatagt gacatattcc
    11581 cccagttggc agtaattagt cagtacaatg ccaatcgggt gtggtaaaga aaggccttta
    11641 ccaatacggg tttttaacga gatatcatta cagtacttac gattattgct agcaccgatc
    11701 cgaataccta atttatgtaa aaagcgatgg ggagagaaat aaaaatactg tgctacccgc
    11761 caccaaaata ggtaacggga acgctggcag aaaaatactc gatgaaatag tcttaaccaa
    11821 ctaaagggtt ttttatcacc aatatattca gcctgtaagc aatcttttag ttcagcaagg
    11881 gtaaccttag cttgagcatg catagttaaa taccatcacc acactcaaag ccgggtacaa
    11941 caccattaaa gtgttgatcc atatcgaatg acggtttttc actatcagga cgaccgacaa
    12001 tgcgggcagg cacccccgcc accgtagtat gagccggtac ttcatgtaat actaccgagc
    12061 cagcaccaat tttggcacca cgaccaatct cgatattacc aaggatttta gctcctgcgc
    12121 caatcattac cccttcacgc actttcgggt gacgatcgcc ccctgtttta cctgttcccc
    12181 ctaaggtcac agattgtaaa atggagacat cattttcaac caccgccgtt tcaccaatca
    12241 caatacctgt cgcatggtcg agcataatac cgtgtccaat acgtgccgct gggtggatat
    12301 ccaccccaaa ggtgactgat atttggtttt gtagataaat agctagcgct tggcgatttt
    12361 gttgccatag ccaatgggcg atacggtagg cttgtagtgc atgaaaccct tttaaataga
    12421 gcagtggggt agaatattta tccaccgcgg gatcacgtag gcgtaccgcg gagagatcac
    12481 aagcagccga ctcaatcatt gagctatcgt gactataagc ctcttcaacg atttcgcgta
    12541 ctgcaatggc tggcatagtc tgagttgcca gcttattggc cagcatatag cttaatgcac
    12601 tacctaaatt ttcgtgtttg agaagtgtcg catgaaagaa actggctaac attggctcac
    12661 aatctgccaa tgcacgcgct tcgcttttaa tatgatccca gactcttgtt agttcttcta
    12721 gcgacatact cactctcatt ttttacacca aagcctaagt aaatacttag gctttgaatt
    12781 ttcaaacaaa tggatagtta aaacagatac cctaagaata ttatttaaaa taaactgact
    12841 acaggctgct gtctatctca tctttggttg ctcgccctaa taaagccaat gcagcctctt
    12901 ttacatcttt atgttgatat aaaatttggt agatctgttc ggtgattggc atttctacac
    12961 ccacttgttc ggctaatgcg cgaacttctt tggtgttacg ataaccttcg actacctggc
    13021 caattttctc ttgggctgta tcaacatcta aaccttgacc tagcatcata ccaaagcgac
    13081 gattgcgtga ttggttgtca gtacaggtta aaaccaaatc acccaaacca gccatgccca
    13141 taaaagttgc cgcatctgca cctaaggctt tacctaagcg gctcatttcg gccagaccac
    13201 gggtaatcag tgcggtacgc gcattagcgc caaatcccat accatcagac atacccgcac
    13261 caatagcaat cacgttttta acagcgccac ccagttgcac accgataaaa tcagggtttt
    13321 tatagactcg gaaactttta ccacaatgaa atagctgttg taactgctct aaaaagagat
    13381 tatccgtcga ggccactgca atcgcggtgg gcaaacccgc ggcaagctct ttagcaaaag
    13441 tagggccaga taatactgcg agcgggattt cattacctaa tacttcacgg gcaacatctt
    13501 gtaataagcg accagtatgt gcttcaagac cttttgtcgc ccaaacaaca cgtgcatcct
    13561 gacgtaaaaa gggtttgatt tgttgcagta cttcaccaaa cacatggctt gggatcacca
    13621 caagaatatt acggctcgct tcaatcgctt tttgcaaaga agcttccata tataaactat
    13681 caggaaagga aacatcgggc agaaaggctt gattacagcg cgcttgttct aatgccgcaa
    13741 cgtgcttggg atcatgcccc cacagcacaa catcgtgccc attacgcgct aaggtaatcg
    13801 ctaaagcggt gccgtatgaa ccggcaccga taactgtcat agaagcgttc attaagcttc
    13861 ttcaactcct gagtttgctg catcctgttg ctgttgttgc tgtaagtagt tcataaacaa
    13921 cgcatcaaag ttaac
//
