LOCUS       KY710704               15073 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 52/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710704
VERSION     KY710704.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 15073)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15073)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15073
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 52/57"
                     /serotype="O29a"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..15073
                     /note="O antigen gene cluster"
     gene            98..619
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             849..1847
                     /note="UDP-galactopyranose mutase"
                     /codon_start=1
                     /transl_table=11
                     /gene="glf"
                     /product="glf"
                     /protein_id="AXY99609.1"
                     /translation="MEHVYGPHIFHTSDEVVWEYINKHGEMIPYVNRVKTTYQNQVYS
                     LPINLHTINQFYQKALNPEQAKTFLSTITDKSITDPKNFEEQALKFIGKDLYEAFFKG
                     YTVKQWGCDPKNLPASILKRLPVRFTYDDNYYSHKYQGMPKHGYTDIVRSILDHKNIQ
                     VITNKKYDHSESKEYDHIIWTGKIDEWFGYCEGRLNYRTLDFIKHETIGDYQGTAVMN
                     YGDIAVPYTRIHEHKHFSPWESHDKTIYIEEHSRVCEENDIPYYPIHLEEENKILNKY
                     LELAQKEKYITFAGRLGTYRYMDMDVSIKEALMLAENLVSDLINQNELKSFYHKHH"
     CDS             1862..2620
                     /codon_start=1
                     /transl_table=11
                     /gene="wemA"
                     /product="wemA"
                     /protein_id="AXY99610.1"
                     /translation="MSKFLAVIRCGDNSLHKNWVNDNAQFDVILSYFGENIPYSLDNI
                     KYVHHFKGSKWEGLYDLFHNHPELWADYDYIWLPDDDLDSTVENINLFFELMQKYRFD
                     LCQPALTNNSYYSYKDLLQEPDLIYRETNFVEVMAPCFSKKIISKAYQTFNENKSGWG
                     LDFYWPILFKEENIKVGVIDSTPIHHTRPVGIAGHGSSDKTLSPLNELHQLLDKYSLE
                     IPTFVATSLLTQKGKMIQKGSFSYSLREFMKKRK"
     CDS             3117..4529
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99611.1"
                     /translation="MNNKKTISSFKWSAIEKLSVQIIQLGVMLILAKLLGPEAFSLIG
                     MLSIFIAIAQVLTEGGVTNAIIRKENRNKSDFTTAFYFNITTSITCYFIIFFSSPYIA
                     LFYEQEKLELLIKIISLVVIINSFSIIPKIKFSINLDFKNQARASLISVFFSSILAIS
                     TSYLNFGVWALVIQSISYSSINCILLNCYSKWYLNGLPSKESLSYLFKFGSKLIIVNI
                     LDAVFNNIYQVVIGKSYPLKQVGYYSQANTLAYTPSFSLTTIIIRVIFPLLSKEQNNK
                     ASFISLYIKILEKTSLFLFPIILSIGIIGFPFISIVLGSQWENTSILLLILAFSYITY
                     PIYSINTHTLQIKGYPGSFLKIEIIRKILICILLLITAPIGVTEICIGIMIQSYITLF
                     ISMIYINKKIKVRFVIQLKKIITPYLITIICGVLSWWVSSFYDNNLIKIILYLTMFFL
                     LYCLIIIIIFKPKHLIPYMK"
     CDS             4533..5708
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /gene="wzy"
                     /protein_id="AXY99612.1"
                     /translation="MPKIFSLKLKELLVILYLASLMMPSLGIKISSIGTLKVSMIISY
                     FIIFIFHNSKVNLRNFIPILLILTAYLLTFLWSVSYSNSIIGAIGIIQLIIYSFSFLL
                     IINHYNRSIFIKLLLSSIVIVCVFSIIYYILGIITYNGDYSENAKNLGIMIDRSMIRA
                     KGFTDDPNIFSLYVGICLIISALNPIKKNKNFILFLLLITFLLTLSRGGIIAMIIAIS
                     ATTIVKLFLSPQQEIKKVILVLLIIILAVAYIILFNDTLLNIIVKRLNNINSASGRFE
                     LWSLAFKAFHERPIFGHGIFTTKYIFEQILGKPAYTHNTFIELLLEGGSYLIFSFAAF
                     LFYLLTQIFNKIKDNTWLFCIFLFIIIQFMTLSLSFNEFIYFLFSFILIYSSRKNLR"
     CDS             5716..6765
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99613.1"
                     /translation="MIYFLLQNANTIGGVENTTKNIVNILNERGKKNINILSIFGKHE
                     EKEERITYLFTSKKFRYIKYFLSFLYMKRNKASIVITNYFIFNVLNIIFSHIFNYKCI
                     IQEHSSFYHDSAYKKVLKKLFYKYADKFLVLNDYDKKLYNSLSLYPIVMKNSIGEMNI
                     NKQLDENNNIEQDEYFLIASRIDENKRVELAIKSFIIYKLLGGKRKLLICGDGDLSLS
                     LKQRYSKIKDILFLGMKKNIYIYIKKASCLLITSKLECLPTSIIEAKYLSTPTIAFSV
                     PSGIPEMITNNVDGFLIKDNSIYEYAEKMLSLDNPSILYSMKKNATLSYSKYSEKLSY
                     DFYLNLLTSLEERNI"
     CDS             6768..7586
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99614.1"
                     /translation="MKLEIIISTLLDGIFNIKINNNFNYLIIHQIPNKNNKKYEQFIK
                     NLPSNVRYISTNTVGLSKSRNIGLNNAQGEYIWIMDDDVIILDSSLGEFDKMINTYQN
                     IDMYVVRYTNKLPFEKGKGKGKGKGKGKVINRFTAMSISSINMIIRRKSLGMIRFNEC
                     FGLGSTYPSGEEYIFSYEFLKHKKTIIKYNEFFSYHKEISSGHDFYSTENKLIAKLAM
                     FIYCYGNIRGRIIYLLFIIKKYKTLVNNKKLLTALNKIRKKIIFEKEYNISNEK"
     CDS             7658..8416
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99615.1"
                     /translation="MLLASKNDYLVSIIMPCFNSANFIQESIDSVLSQTYHNFELIII
                     DDASNDDSLDIIKSFSDNRIKIIELKKNQGAGVSRNKGIEMAQGRFIAFLDSDDIWHK
                     DKLSLQIKFMLNNKYHLTYTNYQCFDKSGIKNTKHPPKSTNYDNLLYCNVIGCLTAIY
                     DSHLIGKQYMPLIRKRQDMGLWLNIMKKYGPAYCLPKTLAYYRIDSGMTQNKINAAIY
                     QWIFYRKELKFNIFKSTKYFIGYAYNGLIKNKVK"
     CDS             8419..9366
                     /note="UDP-N-acetylglucosamine 4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gne"
                     /product="gne"
                     /protein_id="AXY99616.1"
                     /translation="MITIIGGSGFIGTRLSDQLTKENIEFKIVDIAKSDKYPEKWVFG
                     DVTKPESLLEPLKGSNVIINLAAQHKDNVYPISLYYEVNVDGAKNVCDIAEQLNIKHI
                     VFTSSVAVYGFVEKETGEDGEFHPFNDYGKSKLEAEHKYEAWYKKDNTNTLIVIRPTV
                     VFGEGNRGNVYNLFRQIASGKFLMVGSGNNKKSMAYVENIAAFLKFATKIESGHHIFN
                     YIDKPDFTMNELTDIIYTALDKRHSHIKVPYAIGLLGGYCFDILAKVTGKEFPVSSIR
                     IKKFCARTQFKSNSIANTHFKAPVSLEQGIANTVKNEFM"
     CDS             9600..10766
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99617.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNQKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNSNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQKGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEDTFFNSKLIRDLDAFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     CDS             10778..11281
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXY99618.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(11419..12396)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXY99619.1"
                     /translation="MKNNMPKITVLMSVYNDEKYLQESIDSILNQTFKEFEFLIINDG
                     STDNSLSILKENAKKDPRIKLIINEQNIGLIASLNKGLQLASCDLVARMDSDDIAVST
                     RLEAQYQFMLQHPEVSVCGGAFIYQEEPNRINTYPETHSDIVISSLYDCSLAHPTIIY
                     RKQDILSVGGYDKADLYAEDYGLWVNLIIAGKFKLHNLPVPLLYYRTHPNVERSQYKA
                     IQKDTAERVGRKLLMYFGINVNKEDYSLLLDNDKKTVEQVVRCKRLLEKINQQFNAKE
                     YRKELIQKNEFKKKLLERKVLVTDFSSFLIKISARWYFLIRKITKKAKK"
     CDS             complement(12406..12963)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemH"
                     /product="wemH"
                     /protein_id="AXY99620.1"
                     /translation="MHAQAKVTLAELKECLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGDHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGEGYRYINKMNFEINKYC"
     CDS             complement(12966..13799)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99621.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(13898..14911)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99622.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDLIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
     gene            complement(14911..15072)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5288 a   2446 c   2331 g   5008 t
ORIGIN
        1 ggctgcccag tgcattatta cgtcgttgta gcggtgaaag taaagagcta gagcgtattg
       61 aaacagaaac acagaggcta gatggcatga tcaatgactt tatttagtgc tttctcgcaa
      121 ccagtataaa aatgagttat tacgcgaaac cgtaaaagcg aatgagcttt ggaacgatat
      181 tttagataat gcgaaatctg aggcagaaca aagcaataaa accctaacag taaccgcccc
      241 accaggccca tggacaattt attgcaatcc ttattcatta gcgagtgcct ttgaaaatat
      301 cgttcgcaat gccttgcgtt attcacattc tcgtattgaa gtggccttta cagaacaaaa
      361 tcagggtatc actattattg ttgatgatga tggcccaggg gttagcccag aagatcgcga
      421 gcatattttc cgaccttttt atcgtacgga cgaagcacgt gatagagaat ccggcggtac
      481 aggactagga ctggcaattg ttgaaaccgc aattagtcaa catcgaggcc atgttaaagc
      541 tgacgatagc ccattagggg gattaagagt ggagatttgg ttgcctaggg gaagtattag
      601 ctcaagcaat aaattataat tatcattttt aactattgat aattactgta tattttacct
      661 tattaattta tacgaaatgt actaagttct tagggtaaaa tatgaaattt ttagtcgtag
      721 gctgagggtt aacaggggct gtaattagtc gccatctagc tgatgctggt cattgcacca
      781 cgataataga ccaaagatca catattgctg gtaattgcca tacagaacga gatagaaata
      841 ccggtattat ggaacatgtt tatggtccac atatcttcca cactagcgat gaagtggtat
      901 gggaatatat caataaacat ggtgaaatga tcccttatgt aaatagagta aaaacaactt
      961 atcaaaatca agtatattct ttacctatta acctacatac aataaatcaa ttctaccaaa
     1021 aagcgctaaa tccagaacaa gcaaaaactt ttttaagtac tatcacagat aaatctatta
     1081 ctgatcctaa aaactttgag gaacaagccc taaaatttat tggaaaagat ttatatgaag
     1141 ccttttttaa aggctataca gtaaaacaat ggggttgcga ccctaaaaat ctacctgcga
     1201 gtattttaaa acgcctccct gtaagattta cttatgatga caattattat tctcataaat
     1261 accaaggaat gcctaaacat ggctataccg atatagttcg ttctatcctt gatcataaaa
     1321 atatacaagt aataacaaat aaaaagtatg accatagcga aagcaaagaa tatgaccata
     1381 ttatatggac tggaaaaata gatgaatggt ttggctattg cgaaggtaga ttaaactatc
     1441 gaacgctaga ttttataaaa catgaaacta taggtgacta tcaaggtaca gctgttatga
     1501 attacggcga tatcgcggta ccttatactc gcatacatga acataaacac ttctcaccat
     1561 gggaatctca tgacaaaacg atttatattg aagaacatag tcgggtatgc gaagagaatg
     1621 atattcctta ctatcccatt catttagaag aagaaaataa aatattaaac aaatacttag
     1681 aattggctca aaaagaaaaa tatatcactt ttgcaggtag gctaggtaca tatagatata
     1741 tggatatgga tgtatcaatt aaagaagcac ttatgcttgc agaaaactta gtatcagatt
     1801 taataaatca aaatgaactt aagtcgttct accacaaaca ccattaacaa gagaatattc
     1861 tatgtcaaaa tttttagcag taattagatg tggagataat tcacttcata aaaactgggt
     1921 taatgataat gcccaatttg atgtaattct tagttatttt ggtgaaaata tcccttattc
     1981 tttagataat ataaaatatg tacaccactt taaaggatca aaatgggaag gattatacga
     2041 tttattccat aaccatccag agttatgggc tgattatgat tatatctggt tgcctgatga
     2101 tgacttagac agtaccgtag aaaatatcaa cttatttttt gaattaatgc aaaagtatcg
     2161 attcgatctt tgccaaccag cattaacgaa taatagttac tattcctata aagacttact
     2221 acaagaaccg gatttaatat atagagaaac taattttgta gaagtaatgg cgccctgctt
     2281 ttcaaagaaa atcatttcta aagcatatca aacatttaat gaaaataaat cagggtgggg
     2341 attagatttt tattggccta ttttatttaa agaagaaaat attaaagttg gtgttataga
     2401 ctctacacca atacatcata cacgaccagt agggatagca ggccatggga gtagtgataa
     2461 aaccttatcg cctttaaatg aacttcatca attattagac aagtattcac ttgaaatacc
     2521 tacgtttgta gcaacttcac ttctaaccca aaaagggaaa atgatacaaa aaggcagttt
     2581 cagctattct ttaagagagt ttatgaaaaa aagaaaataa ccctatgatt aaattaagaa
     2641 atttaccata aaatcacaat aaaactactt ttatatcaac cataaggact gtttttatat
     2701 tgaataaatg cagtctaatt acctgataca aagtagatac attttgagta atgtagtttt
     2761 aacacctaca acaatacaat aaaaaaatta ctacattaaa atattctgcc atctattgat
     2821 gaataggtgg catattcttt ttattcacat ctttgtagat atttcagaat gatcttgcta
     2881 aatcagtcga tattccgata cttatatata cctaatcaat gttagaatac ccatcaattg
     2941 cttgacccgc tggtttgaag agtctaatct caaagcggtt ttgcatactg ttaaagtatg
     3001 cttatataac aaaactctta tattacagat agataagaga ttttcgagaa aattttggcg
     3061 ctctgggggc cgtaaaccca gtgcggtagc tattaacgtt cttagtataa aaaattttga
     3121 ataataaaaa aaccatatcc agctttaaat ggagtgcgat tgaaaaatta tctgtacaga
     3181 taatacaatt aggcgtgatg ttaattctgg caaaattact agggccagaa gcatttagtt
     3241 taataggaat gctctctata tttatagcaa ttgcacaagt attaactgag ggtggagtca
     3301 caaatgccat cataagaaag gagaatagaa ataaatctga ttttactaca gctttttatt
     3361 ttaacattac tacatcaata acatgctatt ttataatatt cttctcttct ccatatattg
     3421 cattgtttta tgaacaagaa aaattagagt tattaattaa aataatttct ttagttgtaa
     3481 ttattaattc attttctatt attccaaaaa taaaatttag tataaattta gattttaaaa
     3541 accaagcaag agcctcctta attagtgtat tttttagctc catacttgct atctcaacta
     3601 gctatctgaa ctttggagta tgggcattag ttattcagag tattagttat tcatctatta
     3661 attgtattct tttgaattgt tattcaaaat ggtatttaaa cggattgccg agtaaagagt
     3721 ctttaagtta tttatttaaa tttggttcaa aattaataat tgtaaatatc ctagatgctg
     3781 tatttaataa tatatatcag gtagtaatag gaaaaagcta tccattaaaa caagttggct
     3841 actattctca agctaatact ttagcctaca caccatcttt tagtttaaca actataatta
     3901 tacgtgttat atttcctttg cttagtaaag agcaaaataa taaagcctct tttatttctt
     3961 tatatataaa gatcttagaa aaaacatctt tatttttatt ccctattatt cttagtattg
     4021 gaataatagg ctttcctttt atatcaatag ttctcggaag ccagtgggaa aacacttcga
     4081 tattattatt gatcttagct tttagctata taacatatcc aatctattca ataaacaccc
     4141 atactttaca aataaaagga tatccgggtt cttttttaaa gatagaaatt ataagaaaaa
     4201 tattaatctg tatattatta ttaataacag cacctatagg agtaactgaa atttgcatag
     4261 gtattatgat ccaatcatat attacactat ttattagtat gatatatata aataaaaaaa
     4321 ttaaagtacg ttttgtaata cagcttaaaa aaattattac tccttattta ataactatta
     4381 tatgtggagt tcttagttgg tgggtatctt ctttttatga taataactta atcaaaatta
     4441 tattatactt aactatgttt tttttattat attgtttaat tataattatt atttttaaac
     4501 ctaaacattt aattccttat atgaaataaa atatgcctaa aattttctct ctaaaattaa
     4561 aagaattact tgttatttta tatttagcta gtttaatgat gccatcactc ggtataaaaa
     4621 tatcttcaat tggtacttta aaagtatcga tgatcatatc atatttcatt atattcatat
     4681 ttcataactc taaggttaac ttaagaaatt ttatacctat tttattaata cttacagctt
     4741 atttactgac gttcctttgg tctgtaagct acagtaattc gattataggt gctattggga
     4801 taattcaact aatcatatat tcattttcat ttttattaat aataaatcat tataatcgtt
     4861 ctatatttat aaagttatta ctttcatcaa tagtcatagt ttgtgttttt tctattatat
     4921 attatatatt gggcataata acttacaatg gtgattatag cgaaaatgca aaaaacctag
     4981 gtataatgat agatagaagc atgattagag cgaaaggctt tacagatgat cctaatatat
     5041 tttcattgta tgttggaata tgtcttatta tatccgcact aaatcctata aagaaaaata
     5101 aaaactttat tttattttta ttactaatta cctttttatt aacattatct agaggtggca
     5161 ttatagctat gattatagca attagtgcta caacaattgt aaaactgttt ttatctcctc
     5221 aacaagaaat aaaaaaagta atattagtac tattaataat aatactagct gtagcttata
     5281 taatattatt taatgataca ctattaaaca taatagttaa aagattgaat aatatcaata
     5341 gtgccagtgg gagatttgaa ttatggtcct tagcatttaa ggcctttcat gaaaggccta
     5401 ttttcggaca tgggatattt acaacaaaat atatttttga acaaatatta gggaaacctg
     5461 catatactca taatacattt attgaacttc ttcttgaagg agggtcttat cttatctttt
     5521 cgtttgctgc atttttattt tatttattaa cccaaatatt taataaaatt aaagataata
     5581 cttggttgtt ttgtattttt ttatttatta taattcaatt tatgactctt tctttatctt
     5641 tcaatgagtt catatacttt ctcttttcat ttatccttat ttattcttct cgaaaaaatt
     5701 taaggtaatt taaaaatgat atatttctta ttacaaaatg ctaatacaat tggtggagtt
     5761 gaaaatacga caaaaaacat cgttaatatc ttaaatgaga gagggaaaaa aaatataaat
     5821 atactgtcta tatttggtaa acacgaagaa aaagaggaaa gaataaccta tttattcact
     5881 tcaaaaaaat ttagatatat aaaatacttt ttatcgtttc tctatatgaa aagaaataaa
     5941 gctagtattg taataacaaa ctactttata tttaatgtgc ttaatataat tttctctcac
     6001 atttttaatt ataaatgtat cattcaagaa cattcttcat tctatcatga ttcagcatat
     6061 aaaaaagttt taaaaaaatt attttataaa tatgcagata aatttctagt actaaatgat
     6121 tatgataaaa aattatataa ttcattatca ctatatccga tagtaatgaa aaatagtatt
     6181 ggagaaatga atattaataa gcaattagat gaaaataata acattgagca agatgagtat
     6241 tttttaattg catcaagaat tgatgaaaat aaacgggtag aattggcaat aaaatcattt
     6301 attatttata aactgttggg aggaaaaaga aaacttttaa tttgtggtga tggtgattta
     6361 tccctttcat taaagcaaag atattcaaaa ataaaagaca tactattttt aggtatgaaa
     6421 aaaaacatat acatatatat aaaaaaagca tcatgtctac ttataacatc taaactagaa
     6481 tgtctcccta cttcaataat tgaagcaaaa tatttatcta ctcctactat tgccttttcc
     6541 gttccttcag gtatacctga aatgattaca aacaatgtag atggattttt gataaaagat
     6601 aattctatat acgaatatgc agaaaaaatg ttatctttag ataatccaag tattctttac
     6661 tcgatgaaaa aaaatgctac tttaagttat tctaagtata gtgaaaaatt atcttatgat
     6721 ttttatttaa atcttttaac ctctttagaa gaaagaaata tataaatatg aaattagaaa
     6781 ttataatatc tacattatta gatgggattt ttaatataaa aataaacaat aactttaatt
     6841 atttaattat acatcaaata cctaacaaaa ataataaaaa gtatgaacaa tttattaaaa
     6901 acttaccaag taatgtaaga tatatatcta caaatacagt cggtctatca aaaagtagaa
     6961 acataggcct aaataatgcg caaggtgaat acatatggat tatggatgac gatgtaatta
     7021 tcttagatag ctctcttggt gaatttgata aaatgataaa tacatatcaa aatattgata
     7081 tgtatgttgt gagatatacg aataaacttc cattcgaaaa aggaaaagga aaaggaaaag
     7141 gaaaaggaaa aggaaaagtt ataaataggt ttacagcaat gtccatatca tctataaata
     7201 tgattataag aagaaagtca ttaggtatga ttcgttttaa cgaatgtttt ggattaggaa
     7261 gcacatatcc ttcaggagaa gaatatatat tctcatatga atttttaaag cataaaaaaa
     7321 ccattattaa atataatgag tttttctcat atcataaaga aatatcttca gggcatgatt
     7381 tttattccac tgaaaataaa ttaatagcaa aattagctat gtttatatac tgctatggaa
     7441 atataagagg aagaataatc tacttattat ttataataaa aaaatataag actctagtta
     7501 ataataagaa acttttaaca gccttaaata aaattagaaa aaaaattatt tttgagaagg
     7561 aatataatat ttctaatgaa aaatagccca tatataattt atttaccaaa tttaaatttt
     7621 atttaaatat tataaaagat ataaactagg tttacttatg ttattagcat ctaaaaatga
     7681 ttatttggtt tctattataa tgccttgctt taattcagct aattttatac aagaatcaat
     7741 tgattctgtt ttgtcacaaa cttatcataa ttttgaattg ataattatag atgatgcttc
     7801 taatgatgat agtctagata ttataaaatc ttttagtgat aatagaatta aaataattga
     7861 attaaaaaaa aatcaaggtg caggtgtttc tagaaataaa ggaatcgaga tggctcaagg
     7921 aaggtttatc gcttttttag atagtgatga tatatggcat aaagataaat tatcattaca
     7981 aataaaattt atgctaaata ataaatatca cctaacatat accaattatc aatgctttga
     8041 taaatcagga atcaaaaaca caaaacaccc accaaaatca acaaattatg ataatttgtt
     8101 gtattgtaat gttatcggtt gtctaacagc tatttatgat agccacctta ttggtaaaca
     8161 atatatgcca ctaattagaa aaaggcagga tatgggacta tggttaaata ttatgaaaaa
     8221 atatggtcct gcttattgtt tacccaaaac tctagcttat tatcgaattg attctgggat
     8281 gacccaaaat aaaattaatg cagctattta ccagtggata ttttatagaa aagaattaaa
     8341 atttaatata tttaagtcaa caaaatattt tataggctat gcttataatg gattaataaa
     8401 aaataaggta aaatagaaat gataacaatc atcggtggct caggctttat tggcactcgc
     8461 ttatcagatc aattaactaa agaaaatatt gagtttaaaa ttgtggatat tgctaaaagc
     8521 gataaatacc ctgagaaatg ggtttttggc gatgtcacta agccagaatc tttactcgaa
     8581 ccattaaaag gctctaatgt aattatcaat ttagcagcac aacataaaga taatgtttat
     8641 cctattagct tatattatga agttaatgtc gatggtgcta aaaatgtttg tgatattgcc
     8701 gaacaattaa atattaaaca tattgtattt acttcatctg ttgctgtata tggttttgtc
     8761 gaaaaagaaa caggtgaaga tggtgaattc catccattta acgattatgg aaaatcaaaa
     8821 cttgaagctg aacataagta tgaagcttgg tataaaaaag ataatacaaa tacattaatt
     8881 gtaatcagac caacagttgt atttggtgaa ggcaatagag gaaatgtata caacctattc
     8941 cgccaaatag cttcaggtaa atttttaatg gtaggttcag gtaataataa aaaatctatg
     9001 gcttatgtag aaaatattgc agctttctta aaatttgcta ctaaaataga atctggtcat
     9061 catatattta attatataga taagcctgat tttactatga atgagttaac cgatattatc
     9121 tatactgctc tcgacaaacg ccatagtcat attaaagtac cttatgctat tgggcttctt
     9181 ggtgggtatt gttttgatat tctggctaaa gttacaggta aagagtttcc tgtaagtagt
     9241 attcgaatta aaaaattctg tgcacgtact caatttaaat caaatagtat tgctaacact
     9301 cattttaaag cgccagtctc tcttgaacaa ggtattgcga atacagttaa aaatgaattt
     9361 atgtaataaa taaattccac aatatctttt tattaacaaa taattaaaat acaccctaca
     9421 cttctcgact taattttcta atcaaaaatt aattttattt taattgtata agtatatgat
     9481 aaatattgtc gctcaataca ctataccttt actaaaattt gaagaaaaac actaaaatat
     9541 atttttattc tagtatagtg aatttatata aatacatata ctttagataa gagaaacaca
     9601 tgaaaatcac catctcagga accggctatg tcggtctatc taacgggata ctcctttccc
     9661 agcacaacaa agttgttgca ttagatatca ataaagaaaa agtcgaacta ttaaaccaaa
     9721 aaatctctcc tatctctgat aaagaaattg aagagttttt agccactaaa gagctcgact
     9781 ttacagcaac tttagataaa gaattcgctt ataaagatgc tgaatttgtt atcattgcca
     9841 ctcctaccga ttatgatcct aaaactaact actttaacac ctcttctgta gagtcggtta
     9901 ttcgtgatgt tttagaatat aattcaaaca ctactatgat tataaaatca accattccag
     9961 ttggttttac taaacagatg agagaaaaat atcataccga taatattatc ttctctcctg
    10021 agttcttacg tgagggtcgc gccctttacg ataaccttta tccatctcgt attgtgattg
    10081 gtgagcaatc tgcaagagca gagaaatttg ctgagctttt acaaaaaggt gcgttaaaaa
    10141 aagatattga tgttcttttt actgattcta cagaagcaga agccattaag ttatttgcca
    10201 atacttatct cgcactacgt gttgcttatt ttaatgaact cgatagctac gcccaagctt
    10261 acggcttaaa cgcaagacaa attattgaag gtgtctgttt agatccgcgt attggtaatc
    10321 actataacaa cccctcattt ggttatggcg gttactgctt accaaaagat actaaacaac
    10381 tgttagccaa ctacgaatcc gtgcctaata atattattgg cgccattgtg gaagctaacc
    10441 gcacccgcaa agactttatt gctgattcta ttatcgctaa atcaccgaag attgttggtg
    10501 tttatcgttt aattatgaaa tcaggctctg ataatttccg ctcatcttca atccaaggca
    10561 ttatgaagcg tattaaagcg aaaggcattg aagtggtaat ttatgagcca gaaatgaaag
    10621 aagatacttt ctttaactca aaactcattc gtgatttaga tgcgtttaaa gcaatgtctg
    10681 atgtcattat taccaataga atggcttctg cactaaacga tgttgaagaa aaagtttata
    10741 ctcgtgatct ctttggtaac gattaaaact aatttatatg ctcaatatcg tcttatttga
    10801 acccgaaatt ccaccgaata cgggtaatat tatccgcctg tgtgccaaca caggctttca
    10861 tcttcatttg atccaaccat tgggttttac ttgggatgat aaacggctac gccgtgcagg
    10921 gcttgattat cgtgagtttg ctgatatcaa acagcatcac gattattatg cttttttaga
    10981 aagtgaagga ttaagccctg ataatgcaca atccccgaca ggtgcacggt tgtttgcatt
    11041 aaccactaaa ggtactcccg cccatagtaa tgtaagttat caagatgggg attatctgct
    11101 atttggtcct gaaacacgag gattaccacc ttatgtgttg gataatatgc caacacaaca
    11161 aaaaatccgt gtaccaatgt tagcagatag ccgcagtatg aatctttcta atacggttgc
    11221 cgtggttgtt tttgaggcat ggcgtcaatt aggctatcct ggtgcattat taagagatta
    11281 atttatcttt ttaatttata accataaaaa atgacctcca gactttattg tcccgaggtc
    11341 atttttatgt atatgctttg tgctagtttt agcttttctt attttatatc ttctttttat
    11401 cttttttatc tttcggtttt atttcttagc ttttttagtt atttttctta tcaagaaata
    11461 ccatcttgca cttattttta ttaaaaatga tgagaaatca gttactaata cctttctttc
    11521 taataatttt ttcttaaatt catttttttg tattaactct tttcgatact ctttagcatt
    11581 aaattgttga ttaatctttt caagtaacct tttacagcgt actacttgct ctactgtttt
    11641 tttatcatta tccaataata atgaataatc ctctttattc acgttaatgc caaaatacat
    11701 taataatttt cgtcctactc tttccgcggt atctttttgt atcgctttat attgcgaacg
    11761 ttcgacattt ggatgagtac gataataaag aagtggtaca ggaagattat ggagcttaaa
    11821 ttttccagca ataattaagt tcacccataa accataatct tctgcataga ggtctgcttt
    11881 atcatatcca ccaacagata atatatcttg cttacgatag ataatggttg gatgcgctaa
    11941 ggagcaatca taaagactag aaatcacaat atcactatgt gtttctggat aagtattaat
    12001 tctatttggc tcttcttggt agataaaagc accaccacaa acactcactt caggatgttg
    12061 taacataaat tgatattgcg cttctagtct ggttgaaacg gcaatatcat cagagtccat
    12121 tctagcgact aaatcacatg aagctaattg taatcctttg tttaaagaag cgatcaatcc
    12181 gatattttgt tcattaataa tcagttttat tctgggatct tttttggcat tttcttttaa
    12241 aatagataat gagttatccg ttgagccatc attaatgata agaaactcaa attctttaaa
    12301 tgtttgattt aatatagaat caatagactc ctgaaggtat ttctcatcat tgtataccga
    12361 cattaatacg gtaattttgg gcatattgtt tttcattatc tattttcagc aatatttatt
    12421 aatttcaaaa ttcatcttat tgatataccg atacccttcc cccacatcct ttaatactaa
    12481 tgcatgggcg ccaataatcg cattatcacc aatattaatc tcaccaccaa taatggatga
    12541 attacagcca atataaacat gatctcctac ttggatattg gcttttcccc ctttctcttt
    12601 tacgccaata gtcacccctt gcataatagt gacatattcc cccagttgac agtaattagt
    12661 cagtacaatg ccaatcgggt gtggtaaaga aaggccttta ccaatacgag tttttaacga
    12721 gatatcatta cagtatttgc ggttattgct agcaccgatc cgaataccta atttatgtaa
    12781 aaagcgatgg ggagagaaat aaaaatactg tgctacccgc caccaaaata ggtaacggga
    12841 acgctgacag aaaaatactc gatgaaatag tcttaaccaa ctaaagggtt ttttatcacc
    12901 aatatattca gcctgtaagc actcttttag ttcagcaagg gtaaccttag cttgagcatg
    12961 catagttaaa taccatcacc acactcaaag ccgggtacaa caccattaaa gtgttgatcc
    13021 atatcgaatg acggtttttc actatcagga cgaccgacaa tgcgggcagg cacccccgcc
    13081 accgtagtat gagccggtac ttcatgtaat actaccgagc cagcaccaat tttggcacca
    13141 cgaccaatct cgatattacc aaggatttta gctcctgcgc caatcattac cccttcacgc
    13201 actttcgggt gacgatcgcc ccctgtttta cctgttcccc ctaaggtcac agattgtaaa
    13261 atggagacat cattttcaac caccgccgtt tcaccaatca caatacctgt cgcatggtcg
    13321 agcataatac cgtgtccaat acgtgccgct gggtggatat ccaccccaaa ggtgactgat
    13381 atttggtttt gtagataaat agctagcgct tggcgatttt gttgccatag ccaatgggca
    13441 atacggtagg cttgtagtgc atgaaaccct tttaaataga gcagtggggt agaatattta
    13501 tccaccgcgg gatcacgtag gcgtaccgcg gagagatcac aagcagccga ctcaatcatt
    13561 gagctatcgt gactataagc ctcttcaacg atttcgcgta ctgcaatggc tggcatagtc
    13621 tgagttgcca gcttattggc cagcatatag cttaatgcac tacctaaatt ttcgtgcttg
    13681 agaagtgtcg catgaaagaa actggctaaa attggctcac aatctgccaa tgcacgcgct
    13741 tcgctcttaa tatgatccca gactcttgtt agttcttcta gcgacatact cactctcatt
    13801 ttttacacga aagcctaagt aaatacttag gctttgaatt tcaaacaaac ggatagttaa
    13861 aacagatacc ctaagaatat tatttaaaat aaactgacta caggctgctg tctatctcat
    13921 ctttggttgc tcgccctaat aaagccaatg cagcctcttt tacatcttta tgttgatata
    13981 aaatttggta aatctgttcg gtgattggca tttctacacc cacttgttcg gctaatgcac
    14041 gaacttcttt ggtgttacga taaccttcaa ctacctggcc aattttctct tgggctgtat
    14101 caacatctaa accttgacct agcatcatac caaagcgacg attacgtgat tgattgtctg
    14161 tacaggttaa aaccaaatca cccaaaccag ccatgcccat aaaagttgcc gcatctgcac
    14221 ctaaggcttt acctaagcgg ctcatttcgg ccagaccacg ggtaatcagt gcggtacgcg
    14281 cattagcgcc aaatcccata ccatcagaca tacccgcacc aatagcaatc acgtttttaa
    14341 cagcgccacc cagttgcaca ccgatcaaat cagggttttt atagactcgg aaacttttac
    14401 cacaatgaaa tagctgttgt aactgctcta aaaagagatt atccgtcgag gccactgcaa
    14461 tcgcggtggg caaacccgcc gcaagctctt tagcaaaagt agggccagat aatactgcga
    14521 gcgggatttc attacctaat acttcacggg caacatcttg taataagcga ccagtatgtg
    14581 cttcaagacc ttttgtcgcc caaacaacac gtgcatcctg acgtaaaaag ggtttgattt
    14641 gttgcagtac ttcaccaaac acatggcttg ggatcaccac aagaatatta cggctcgctt
    14701 caatcgcttt ttgcaaagaa gcttccatat ataaactatc aggaaaggaa acatcgggca
    14761 gaaaggcttg attacagcgc gcttgttcta atgccgcaac gtgcttggga tcatgacccc
    14821 acagcacaac atcgtgccca ttacgcgcta aggtaatcgc taaagcggtg ccgtatgaac
    14881 cggcaccgat aactgtcata gaagcgttca ttaagcttct tcaactcctg agtttgctgc
    14941 atcctgttgc tgttgttgct gtaagtagtt cataaacaac gcatcacagt taacaggtgc
    15001 taagtttaat tgtgggaaag taccacgacc cactaagttg gtgatacatt cacgtgcata
    15061 agggaataat acg
//
