LOCUS       KY710705               11880 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 53/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710705
VERSION     KY710705.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11880)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11880
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 53/57"
                     /serotype="O30"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     gene            1..642
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..11880
                     /note="O antigen gene cluster"
     CDS             1084..2007
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99623.1"
                     /translation="MNKLIVSNDLPLVSVIIPTYGRPHFLERAIDSVLSQSYKNIEII
                     VVDDNSSNLHHEQTVKIIKKYNRNNKIIYIFDGINVGGSLARNRGIIESKGEYITFLD
                     DDDIYLEKKIESQVEHIINNNIDVSVCNMFFKKNNNFLDISNCYSRVGSIQEFILNGN
                     AYTPMIMSKKESLLEVGLFTHSPRYQDHILMLKLFMAGKKIKSLNEKLFIHNDHNEKR
                     ITNSNKSEEAYRIRQSYERKNLYKLNDIEKKQYKIIECLINAKILRAKKNYLNSLKSV
                     ISAFTYITNRNDLYKIIKTLIRIHILPNKKI"
     CDS             2009..3115
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99624.1"
                     /translation="MMIKNLKYDPFIIMFFLLYVLYSIKSGFFSLIINNEFIFQIKNF
                     INVFLIISSVTSAFLLSKTRTKFYLAIFTILSIIGLFFFSNKSYLGFLLIISMLILAS
                     KIKWENILKIIIFSNIITIIFICFSAYFSEYYFLEDDRFGERFTAGFDNPNTLGQYSL
                     MLFTVTALFSELKIKEITTRLFIVSVIFIIILTILYLTYSRTSLLLSVLFYFLFLICT
                     IYKKSNQFLPRKKIKTLLFLFSFSIIIIQFYFILFFKIDSFLYEVNDLLTSRLWFGNI
                     LFNSIGFPNFFIGTNIEEYLPIDFYFIQIIYSFGLIPFSILYFMITKKFFNTKITLLM
                     GCTLFVMLLETMTETYFSVPFYSIALFIIYSKKI"
     CDS             3117..4187
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99625.1"
                     /translation="MKKLLFFITDITTGGGTERALITLVNKLADKNFNIYIYSLYRKN
                     TTPLFEINKKIKIEFLKSKHHIIKLFNMKNIAQEDPSAIKIIVSMGRLSIETALLFKL
                     FKITNIFLSEHTSFESYRFLIRKLKLLSYRLADKVIVLTEHDKKLLNSEYNLNNIYTI
                     PNINPYEEYKYNFNFDQRKNIALAIGRLCYAKNFERLIQLWSKANTQNWKLLIVGDGN
                     DRKKLEKTINETGVKNIEIFPFTKDIISYYQKAKLYLMTSRFEGLPMVLIESQHFSIP
                     IISFNCKTGPSEIVKNDYNGYLVDYNNDSLFIEKLDFFIRNESLQRKLAINSPLVSSN
                     YSSKIIINMWENILSENKSYEK"
     CDS             4177..4998
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99626.1"
                     /translation="MKNNTICVVIPIYINTNIHLFKKAIESIVQQSHPADEILIICDG
                     LISTQLNNLIKTFSNKYSYIKIIRYDHNKGPGYARDYGIKCTSCDYIAIMDSDDVSTI
                     DRLAIQYEFMQKNNDISLCGGLIRELDDNNHKISIRNVPMLHSEILKTIKIKSPINNV
                     TAFFRRKDYLETDGYAHIRSSEDYALWARLIYKEKKLQNLPHIFVDVNFDIGSLSRRT
                     GLAHFKNDLFTQKELLNFNLITRYQFYKNILKYFIFRCILHKNIKKILYKYYLRK"
     CDS             5006..6175
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99627.1"
                     /translation="MYKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNKKIS
                     PISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVI
                     RDVLEYNPNTTMIIKSTIPVGFTKQMREKYNTDNIIFSPEFLREGRALYDNLYPSRIV
                     IGEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSY
                     AQAYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGA
                     IVEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVV
                     IYEPEMKEETFFNSKLIRDLDEFKAMSDVIITNRMATALNDVEEKVYTRDLFGND"
     CDS             6279..7535
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99628.1"
                     /translation="MNKKILHNAIWMISEKAISIIGLFLVTSYVAKYVGPETFGIIAL
                     ATATFQIVQIIAQLGNDNIIFKRVSKNNRSGIKLIKSTFLIRTIIYITITIPLLIYFY
                     SYKNNLTFIFSIAISVACYFSSIDVYSIYNNAILKSKQNTISNMVGLTLGLSIQFIIA
                     YKKYNPIYLSIPIIITTFLPFLIRFILFNKMNLVKEIVVKKKSRYNKYILSSGLAIVF
                     SSLSIAIYTRINQFMISYLDSDYSLGIYSVAVTLSSSWAFILSALISSTLPSIFGEKD
                     DNKAIRIGVKLNIIITLISLLVLSFITLFGKLFINLLFGEEYINAYYLLKILCISTMF
                     SNLGMLSSRFIVRSSGYSFLSKKMFIMVLLSIPISYCLIKNYGLIGASYSVLIIEFVS
                     LTLMNYFFKKRIVWKLHLETLKMNLK"
     CDS             7559..8062
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXY99629.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYTFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(8198..9175)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXY99630.1"
                     /translation="MKNNMPKITVLMSVYNDEKYLQESIDSILNQTFKEFEFLIINDG
                     STDNSLSILKENAKKDPRIKLIINEQNIGLIASLNKGLQLASCDLVARMDSDDIAVST
                     RLEAQYQFMLQHPEVSVCGGAFIYQEEPNRINTYPETHSDIVISSLYDCSLAHPTIIY
                     RKQDILSVGGYDKADLYAEDYGLWVNLIIAGKFKLHNLPVPLLYYRTHPNIDRSQYKA
                     IQKDTAERVGRKLLMYFGINVNKEDYSLLLDNDKKTVEQIVRCKRLLEKINQQFNAKE
                     YRKELIQKNEFKKKLLERKVLVTDFSSFLIKVSARWYFLIRKITKKAKK"
     CDS             complement(9185..9742)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemH"
                     /product="wemH"
                     /protein_id="AXY99631.1"
                     /translation="MHAQAKVTLAELKECLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGNHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGEGCRYINKMNFEINKYC"
     CDS             complement(9745..10578)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99632.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPMLASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(10678..11691)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99633.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGFDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
     gene            complement(11691..11879)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     4074 a   2000 c   1808 g   3998 t
ORIGIN
        1 cgcaccccat taactcgttt acagcttgcc agtgcattac tacgccgtcg tagcggtgaa
       61 agtaaagagc tagagcgtat tgaaacagaa acacagcggc tagatggcat gatcaatgac
      121 ttattagtgc tttcccgcaa tcaatataaa aatgagttat tacgcgaaac cgtaaaagcg
      181 aatgagcttt ggaacgatat tttagataat gcgaaatttg aggctgaaca gagcaataaa
      241 actctaacag taaccgcccc gtcaggccca tggacgattt attgtaatcc ttattcatta
      301 gcgagtgcct ttgaaaatat cgttcgcaat gccttgcgtt attcacattc tcgtattgaa
      361 gtggccttta cagaacaaaa tcagggtatc actattattg ttgatgatga tggcccaggg
      421 gttagcccag aagatcgcga gcatattttc cgaccttttt atcgtacgga cgaagcacgt
      481 gatagagaat ccggcggtac agggctggga ctagcaattg ttgaaaccgc aattagtcaa
      541 catcgaggcc atgtaaaagc cgatgatagc ccattagggg gattaagagt ggagatttgg
      601 ttgccgagag ggagtgtggt aaataaatca caacacgctt aattcagtaa taaaattact
      661 atattaaatt attctgccat ctattgaaga ataggtggca tattcttttc attcccatct
      721 ttatagatat ttcagaatga tcttgctaat tcagtcgata ttccgatatt tatatatacc
      781 taatcaatgt tagaataccc atcaattact tgaccagcgg gtttgaagag tctaatctca
      841 gagcggtttt gcgtactgtt aaagtatgct tatataataa aactcttata ttacagatag
      901 ataagagatt atcgagaaat tttttggcgc tctgggggcc ataaacccag tgcggtagct
      961 tttctaaaaa ttagactaca tgattaaaaa tgatgactat atatacaaca tctttatatc
     1021 acatctaatt attaatttag aaaatcaata taatatataa acaacagtag taattatacg
     1081 aaaatgaata aattaatcgt atcaaatgat cttcctttag tctctgttat aattcctaca
     1141 tatggaagac cgcatttttt agaaagagca atagacagcg tattatctca atcatataaa
     1201 aacatagaaa taatcgtggt agatgataat tctagtaatt tacaccacga acaaacagtc
     1261 aaaataataa aaaaatataa tcgcaataat aaaataatat acatttttga tggtatcaat
     1321 gtgggtggtt cattagctag aaatagagga attatcgaat ctaaaggaga atatattact
     1381 tttttagatg atgatgatat atatttagaa aaaaagatag agagtcaggt ggaacatatt
     1441 atcaataata atattgatgt cagtgtatgt aatatgtttt ttaaaaaaaa taataatttc
     1501 ttggatatta gcaattgtta tagtagagta ggaagcattc aagaatttat cttaaatggt
     1561 aatgcttaca ctccaatgat tatgagtaag aaagaatcac tattagaagt aggattattt
     1621 actcattccc ctcgctacca agaccatata ttaatgctaa aattattcat ggcaggtaaa
     1681 aaaattaaat cattaaatga aaagctcttt attcataatg atcataatga aaaaagaata
     1741 actaatagta ataaatcaga ggaagcttat agaataagac agtcgtatga aagaaaaaat
     1801 ttatataaac taaatgatat agaaaaaaaa caatacaaaa ttattgaatg ccttattaat
     1861 gcaaagatat tgcgtgcaaa aaaaaattat cttaattcat taaaaagtgt tatttctgca
     1921 tttacctata taacaaatag gaatgatcta tataaaataa taaaaacatt aattagaata
     1981 catatcctac ccaataaaaa aatataacat gatgattaaa aatttaaaat acgacccttt
     2041 tattataatg ttttttttat tgtatgtttt atattctata aaaagtggtt ttttctcatt
     2101 aattataaat aatgaattta tttttcaaat caagaatttt ataaatgtat ttcttataat
     2161 ttcatctgtc acttcagcct ttctattatc aaaaacaaga acaaaatttt atttggcaat
     2221 atttactatt ctatccatta ttggattatt tttcttctct aacaaaagtt atttaggttt
     2281 tttactaatc atatccatgc taatattggc atcaaaaata aaatgggaaa acatacttaa
     2341 aattataata ttttcaaata ttataaccat tatatttata tgtttttccg catattttag
     2401 tgaatattat tttttagaag atgataggtt tggagaaagg tttacagctg gatttgataa
     2461 tccaaatacg ttggggcaat attctttaat gttattcaca gtaacagccc ttttttcaga
     2521 attaaaaatt aaagaaatta caactcgatt atttattgta agtgtaatat ttattattat
     2581 acttacaata ttatatttga catattctag aactagttta ttactatcgg ttttatttta
     2641 ttttcttttc cttatttgca caatatataa aaaaagtaat caatttttac caagaaagaa
     2701 aataaaaact ttactttttt tattttcttt ctccattatt ataattcaat tttattttat
     2761 tttatttttt aaaatagata gttttcttta tgaagttaat gatctcttaa ctagtagatt
     2821 atggtttgga aacattttat ttaattccat aggtttccct aattttttta ttggaactaa
     2881 tattgaagaa tatcttccca ttgattttta ttttatccaa ataatataca gctttggatt
     2941 aataccgttt tctatcttat attttatgat aacaaaaaaa ttctttaaca caaaaataac
     3001 attgttaatg ggatgtactc tttttgttat gctattagaa accatgactg aaacatattt
     3061 ttctgtacct ttttattcaa ttgctttatt tattatctat agtaagaaaa tttaacatga
     3121 agaaactatt attttttata actgatataa caactggtgg tggtactgaa agagcattaa
     3181 ttactcttgt aaataaatta gctgataaaa atttcaatat ttatatttat tctctttaca
     3241 ggaaaaatac aacaccttta tttgaaataa ataaaaaaat aaaaatagaa ttcttaaaaa
     3301 gtaaacacca tattattaaa ctatttaata tgaaaaatat tgctcaagaa gacccttcag
     3361 ctattaaaat tatagtatct atgggaagat tatcgattga aactgcatta ttatttaagc
     3421 tatttaaaat aactaatatt tttttatcag aacatacgtc ttttgagtct tatagatttt
     3481 taattagaaa attaaagtta ttaagctata gactggcaga taaagttata gtattaactg
     3541 aacatgataa aaaattactc aatagtgagt ataatttaaa taatatttat accataccta
     3601 atattaaccc atatgaagaa tataaatata atttcaactt tgaccaaaga aaaaacatag
     3661 ctcttgctat aggaagattg tgctatgcta aaaattttga aagactaata caattatgga
     3721 gtaaagcaaa cacgcaaaat tggaaattac taattgttgg tgatggcaat gatagaaaaa
     3781 aattagaaaa aactattaat gaaacaggtg taaaaaatat agaaattttt ccatttacaa
     3841 aagatattat ttcttattat cagaaagcaa agctatattt aatgacctcg cgattcgaag
     3901 gtttaccaat ggttttaatt gagtcccaac attttagcat acctattatc tcttttaatt
     3961 gtaaaacagg tccatccgaa atagtaaaaa atgactataa tggttaccta gtcgattata
     4021 acaacgattc actatttata gaaaagttag attttttcat aagaaacgaa tcgttacaga
     4081 gaaaattagc tattaactct cctttggttt caagtaatta ttcaagtaaa ataattataa
     4141 atatgtggga aaatatttta tctgaaaata aatcttatga aaaataatac aatttgtgtt
     4201 gttataccta tttatataaa tactaatatt cacctattta aaaaagctat tgaatcaata
     4261 gttcaacaat cacatccagc agatgaaatt ctaattattt gtgatggcct tatatctact
     4321 caattaaata atcttataaa aacattttcg aataaatatt catatataaa aataattcga
     4381 tacgatcata ataaaggtcc aggctatgca agagattatg gaatcaaatg tacttcttgt
     4441 gattatatag ctataatgga ttccgatgat gtatctacaa tagacagatt agcaatacaa
     4501 tatgaattca tgcagaaaaa taacgacata tctttgtgtg gtggattaat aagagaactc
     4561 gatgacaata atcataaaat ttcaataaga aatgtcccaa tgctccattc ggaaatacta
     4621 aaaacaatta aaataaaatc tcctataaac aatgtaacag ctttttttcg tcgtaaagat
     4681 tatttagaaa ctgatggata tgcacatatt agaagctctg aagattatgc tctttgggct
     4741 agattaatat ataaagaaaa gaaactacaa aaccttccac atatatttgt cgatgtcaac
     4801 ttcgatatag gttcattatc cagaagaact ggattggctc attttaaaaa tgacttgttc
     4861 acacaaaaag aattattaaa ttttaattta ataactcgat atcaatttta taaaaatatt
     4921 ttaaaatact ttatattccg ttgtattttg cataaaaata taaaaaaaat actatataaa
     4981 tattatttaa ggaaataaaa ttaacatgta taaaatcacc atctcaggaa ccggctatgt
     5041 cggtctatct aacgggatac tcctttccca gcacaacaaa gttgttgcat tagatatcaa
     5101 taaagaaaaa gtcgaactat taaacaaaaa aatctctcct atctctgata aagaaattga
     5161 agagttttta gccactaaag agctcgactt tacggcaact ttagataaag aattcgctta
     5221 taaagatgct gaatttgtta tcattgccac tcctaccgat tatgatccta aaactaacta
     5281 ctttaacacc tcttctgtcg agtcggttat tcgtgatgtt ttagaatata atccaaacac
     5341 cactatgatt ataaaatcaa ccattccggt tggttttact aaacagatga gagagaaata
     5401 taataccgat aatattatct tctctcctga gttcttacgt gaaggtcgcg ccctttacga
     5461 taacctctat ccatctcgta ttgtgattgg tgagcagtct gcaagagcag agaaatttgc
     5521 tgagctttta caacaagggg cattaaaaaa agatattgat gttcttttta ctgattctac
     5581 agaagcagaa gctattaagt tatttgctaa tacttatctc gcactgcgtg ttgcttattt
     5641 taatgaactt gatagctacg cccaagctta cggtctaaat gcaagacaaa ttattgaagg
     5701 tgtttgttta gatccgcgta ttggtaatca ctataacaac ccatcatttg gttatggtgg
     5761 ctactgctta ccaaaagata ctaaacaact gttagcaaat tacgaatcag taccgaataa
     5821 tattattggt gccattgttg aagctaaccg cacccgtaaa gactttatcg ctgattctat
     5881 tatcgctaaa tcaccaaaga ttgttggcgt ttaccgctta attatgaaat caggctctga
     5941 taatttccgc tcatcttcaa ttcaaggcat tatgaagcgt attaaagcaa aaggcattga
     6001 agtggtaatt tatgagccag aaatgaaaga agagactttc tttaactcaa aactcattcg
     6061 tgatttagat gagtttaaag ccatgtctga tgtcattatt accaatagaa tggctacggc
     6121 gctaaacgat gtggaagaaa aagtttatac tcgtgatctc tttggtaacg attagttttc
     6181 tttctataga gataaaaaga gtataattga aaatactttc aattatactc tactacaatt
     6241 tttatatatt taaaatataa aattaggttt ctactatatt gaataaaaaa atattacata
     6301 atgctatttg gatgatttct gaaaaagcaa tctccatcat aggattattc ttagtcactt
     6361 cttatgttgc taaatacgtt ggtccagaaa catttggtat tattgcttta gcaaccgcta
     6421 catttcaaat agttcaaatc attgcacaat tgggtaatga caacattatt ttcaaaagag
     6481 tatcaaaaaa taatcgttct ggtattaaac taataaaatc tactttcctt atacgcacta
     6541 ttatttatat aactataact ataccacttc ttatatattt ttattcatac aaaaataatt
     6601 taacttttat attctctatt gctatttctg tagcatgtta tttttcttcc atagatgttt
     6661 attcaattta taacaatgcc atccttaaat caaaacaaaa tactatatct aatatggttg
     6721 gattaacttt aggattaagc attcagttta taatagctta caagaaatat aaccctatct
     6781 atttatccat accaataatt ataacaacct ttctcccatt tttgattaga ttcattttat
     6841 ttaataaaat gaatctagta aaagaaatcg ttgtcaaaaa aaaatcaagg tataataaat
     6901 atatattaag ctctggttta gcaattgtgt tctcctcatt atcaattgct atttatacta
     6961 gaataaatca atttatgatt tcttacttag acagtgatta cagtttagga atttattcag
     7021 ttgcagtaac attatcaagc tcatgggcat tcattttatc tgcactaata agttcaacac
     7081 taccttctat atttggagaa aaagatgata ataaagcgat tagaatagga gtaaaactta
     7141 acattattat aacattaata tcattactag ttctttcttt tataacttta tttggtaagc
     7201 tttttataaa tttacttttt ggtgaagaat atatcaatgc ttactattta ttaaaaatat
     7261 tatgtatatc aacaatgttt tcaaaccttg gaatgctatc atctagattt attgttcgtt
     7321 cgtcaggcta ttcatttcta tcaaaaaaaa tgtttattat ggttctactt agtattccta
     7381 tatcttattg tcttataaaa aattatggtt taataggcgc atcatatagt gtattgataa
     7441 tagaatttgt ctcattaaca ttaatgaact attttttcaa gaaaagaata gtttggaagc
     7501 ttcatctaga aacattaaaa atgaatctaa aataacagta actttctaaa ttagaaatat
     7561 gctcaatatc gtcttatttg aacccgaaat tccaccgaat acgggtaata ttattcgcct
     7621 gtgtgccaac acaggctttc atcttcattt gatccaacca ttgggtttta cttgggatga
     7681 taaacggcta cgccgtgcag ggcttgatta tcgtgagttt gctgatatca aacagcatca
     7741 cgattattat acttttttag aaagtgaagg attaagccct gataatgcac aatcaccgac
     7801 aggtgcgcgg ttgtttgcat taaccactaa aggtactccc gcccatagta atgtaagtta
     7861 tcaagatggg gattatctgc tatttggccc tgagacacga ggtttaccac cttatgtgtt
     7921 ggataatatg ccaacacaac aaaaaatccg tgtaccaatg ttagcggata gtcgcagtat
     7981 gaatctttct aatacggttg ccgtggttgt ttttgaggca tggcgccaat taggctatcc
     8041 gggtgcatta ttaagagatt aatttatctt tttaatttat aaccataaaa aatggcctca
     8101 agacttcatt gtctcgaggt catttttata tatgctttgt gctagtttta gcttttctta
     8161 ttttatatct tctttttatc ttttttatct ttcggtttta tttcttagct tttttagtta
     8221 tttttcttat caagaaatac catcttgcac ttacttttat taaaaatgat gagaaatcag
     8281 ttactaatac ctttctttct aataattttt tcttaaattc atttttttgt attaactctt
     8341 ttcgatactc tttagcatta aattgttggt taatcttttc aagtaacctt ttacagcgta
     8401 ctatttgctc tactgttttt ttatcattat ccaataataa tgaataatcc tctttattca
     8461 cgttaatgcc aaaatacatt aataattttc gtcctactct ttccgcggta tctttttgta
     8521 tcgctttata ttgcgagcga tcgatatttg gatgagtacg ataataaaga agtggtacag
     8581 gaagattatg gagcttaaat tttccagcaa taattaaatt cacccataaa ccataatctt
     8641 ctgcatagag gtctgcttta tcatatccac caacagataa tatatcttgc ttacgataga
     8701 taatggttgg atgcgccaag gagcaatcat aaagactaga aatcacaata tcactatgtg
     8761 tttctggata agtattaatt ctatttggct cttcttggta gataaaagca ccaccacaaa
     8821 cactcacttc aggatgttgt aacataaatt gatattgcgc ttctagtctg gttgaaacgg
     8881 caatatcatc tgagtccatt ctagcgacta aatcacatga agctaattgt aatcctttgt
     8941 ttaaagaagc gatcaatccg atattttgtt cattaataat cagttttatt ctgggatctt
     9001 ttttggcgtt ttcttttaag atagataatg agttatccgt tgagccatca ttaatgataa
     9061 gaaactcaaa ttctttaaat gtttgattta atatagaatc aatagactcc tgaaggtatt
     9121 tctcatcatt gtataccgac attaatacgg taattttggg catattgttt ttcattatct
     9181 attttcagca atatttatta atttcaaaat tcatcttatt gatataccga cacccttccc
     9241 ccacatcctt taatactaat gcatgggcgc caataatcgc attatcacca atattaatct
     9301 ctccaccaat aatggatgaa ttacagccaa tataaacatg gtttcctact tggatattgg
     9361 cttttccccc tttctctttt acgccaatag tcaccccttg cataatagtg acatattccc
     9421 ccagttggca gtaattagtc agtacaatgc caatcgggtg tggtaaagaa aggcctttac
     9481 caatacgggt ttttaacgag atatcattac agtacttacg attattgcta gcaccgatcc
     9541 gaatacctaa tttatgtaaa aagcgatggg gagagaaata aaaatactgt gctacccgcc
     9601 accaaaatag gtaacgggaa cgctgacaga aaaatactcg atgaaatagt cttaaccaac
     9661 taaagggttt tttatcacca atatattcag cctgtaagca ctcttttagt tcagcaaggg
     9721 taaccttagc ttgagcatgc atagttaaat accatcacca cactcaaagc cgggtacaac
     9781 accattaaag tgttgatcca tatcgaatga cggtttttca ctatcaggac gaccgacaat
     9841 gcgggcaggc acccccgcca ccgtagtatg agccggtact tcatgtaata ctaccgagcc
     9901 agcaccaatt ttggcaccac gaccaatctc gatattacca aggattttag ctcctgcgcc
     9961 aatcattacc ccttcacgca ctttcgggtg acgatcgcct cctgttttac ctgttccccc
    10021 taaggtcaca gattgtaaaa tggagacatc attttcaacc accgccgttt caccaatcac
    10081 aatacctgtc gcatggtcga gcataatacc gtgtccaata cgtgccgctg ggtggatatc
    10141 caccccaaag gtgactgata tttggttttg tagataaatg gctagcgctt ggcgattttg
    10201 ttgccatagc caatgggcaa tacggtaggc ttgtagtgca tgaaaccctt ttaaataaag
    10261 caatggggtt gaatatttat ctaccgcggg atcacgtagg cgtaccgcgg agagatcaca
    10321 agcggccgac tcaatcattg agctatcgtg actataagcc tcttcaacaa tttcgcgtac
    10381 tgcaatggct ggcatagtct gagttgccag cttattggcc agcatatagc ttaatgcact
    10441 acctaaattt tcgtgtttga gaagtgtcgc atgaaagaaa ctggctaaca ttggctcaca
    10501 atctgccaat gcacgcgctt cgcttttaat atgatcccag actcttgtta gttcttctag
    10561 cgacatactc actctcattt tttacaccaa agcctaagta aatacttagg ctttgaattt
    10621 tcaaacaaat ggatagttaa aacaggtacc ctaagaatat tatttaaaat aaactgacta
    10681 caggctgctg tctatctcat ctttggttgc tcgccctaat aaagccaatg cagcctcttt
    10741 tacatcttta tgttgatata aaatttggta gatctgttcg gtgattggca tttctacacc
    10801 cacctgttcg gctaatgcgc gaacttcttt ggtgttacga taaccttcga ctacctggcc
    10861 aattttctct tgggctgtat caacatcaaa accttgacct agcatcatac cgaagcgacg
    10921 attacgtgat tggttgtcag tacaggttaa caccaaatca cccaaaccag ccatgcccat
    10981 aaaagttgcc gcatctgcac ctaaggcttt acctaaacgg ctcatttcgg ctagacctcg
    11041 ggtaatcagt gcggtacgcg cattagcgcc aaatcccata ccatcagaca tacccgcacc
    11101 aatagcaatc acgtttttaa cagcgccacc cagttgcaca ccgataaaat cagggttttt
    11161 atagactcgg aaacttttac cacaatgaaa tagctgttgt aactgctcta aaaagagatt
    11221 atccgtcgag gccactgcaa tcgcggtggg taaacccgct gcaagctctt tagcaaaagt
    11281 agggccagat aatactgcga gcgggatttc attacctaat acttcacggg caacatcttg
    11341 taataagcga ccagtatgtg cttcaagacc ttttgtcgcc caaacaacac gcgcatcctg
    11401 acgtaaaaag ggtttgattt gttgcagtac ttcaccaaac acatggcttg ggatcaccac
    11461 aagaatatta cggctcgctt caatcgcttt ttgcaaagaa gcttccatat ataaactatc
    11521 aggaaaggaa acatcgggca gaaaggcttg attacagcgc gcttgttcta atgccgcaac
    11581 gtgcttggga tcatgccccc acagcacaac atcgtgccca ttacgcgcta aggtaatcgc
    11641 taaagcggtg ccgtatgaac cggcaccgat aactgtcata gaagcgttca ttaagcttct
    11701 tcaactcctg agtttgctgc atcctgttgc tgttgttgct gtaagtagtt cataaacaac
    11761 gcatcaaagt taacaggtgc taagtttaat tgtgggaaag taccacgacc cactaagttg
    11821 gtgatacatt cacgtgcata agggaataat acgttcaggc aatatgcacc taagcaatgt
//
