LOCUS       KY710706               16772 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus vulgaris strain PrK 55/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710706
VERSION     KY710706.1
KEYWORDS    .
SOURCE      Proteus vulgaris
  ORGANISM  Proteus vulgaris
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 16772)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16772)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16772
                     /organism="Proteus vulgaris"
                     /mol_type="genomic DNA"
                     /strain="PrK 55/57"
                     /serotype="O31ab"
                     /db_xref="taxon:585"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     gene            1..633
                     /gene="cpxA"
                     /note="coding region not determined"
     misc_feature    1..16772
                     /note="O antigen gene cluster"
     CDS             1022..2113
                     /note="orf02"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf02"
                     /product="acyltransferase"
                     /protein_id="AXY99634.1"
                     /translation="MFYSIHYIRGISALLVVLFHLRPSINNVYAQKNLGDLLFLRGDI
                     GVDAFFIISGFIICHATRNDKSIISFSLKRFFRIYPTYLLFSILFLCINLILFNHLTI
                     KSFFDIILIRPLNYSMGPPFYGYTYLVVGWSLVYEICFYIIYLVSMSLSHKYRNIICS
                     LILFLLCYALQKYHLNTFDISATSGLKDKNLGILNILSSSMMLNFIVGIFIYEITKYI
                     KGNLHFINKEFIKIIFFFVITISIYFFMIGFNSWHGLNKSVLFIAPLILSLIIYEYKC
                     GLKRIPAFIFLGNISYSLYLSHLVVTYAIKKFGGPLYNANNGFSTIFIIISLSLIISY
                     ISYNTIEKISSEACRKIIMKIKNSDQENT"
     CDS             2131..3288
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99635.1"
                     /translation="MNIKPKINAIYFFLFGFLYYIVSPVISLYFFDKSWFVLIAKQHV
                     KLNDYGLAYSFISILCLLIFSLAYIIFSNLKLLKTNIGEKEKEHKLLPLIIFIIILSL
                     LLFTIIKSYYSGVSLFSGYNEGYNISLLGPLATISFSSIIFMFYFEKRKYKTGFFIIY
                     LISNVFLLGMGSRMFFLIGLISIAINEYNRNPKIIKTLRFHILSISLFLFILFIGIWR
                     SNSELSLEKLLGIFFAEPLFTSISAINYLHIIENESLIKIPWDVIASFLNFIPSELFK
                     DKIVIISEISYDIKSYSPFGASSLLTNIYINFGILFPIYIFSIGMVFGILSKLSYNKL
                     IYSIYITTLPLLMFHFFREGFITYIKIQFFNGLIFPIFIILLISFLLRVKR"
     CDS             3285..4502
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99636.1"
                     /translation="MIKSILLLLSSSLLSSLLTFITQAYIANQLNPNDFGEFSSSLAL
                     VMLLVPFVAMGADSYLLKIYADKKGNTSEYNSFWIAYFIFTLILSILIYTIFNTTSSS
                     SIILVSILASQALINYISAIYQTKSKYKKLSLIQALQSFFRFFTLIILSYAFELNINL
                     VYYSYFFVSILIIIICIILIIKESNFIAIKNIKNIKLYFFQAIPFGLTPLLHLIYFQS
                     DIILIDRLYSSTEAGFYSAAFMLITASYILPAVIYQKYLLPYIHILNANNDITNEFKI
                     FKTGAFTILPISIIIFLIYYFLSPYIVLVIFGEAYKKTQEIMLILSVCIIFRYLSSHV
                     GLFLTAGNLIKVKNKNMLLCAIINILLNVIFIPSFGSIAAAYITVITEILLLLMFFYS
                     FYIFKIKRFYVIK"
     CDS             4489..5472
                     /note="orf05"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf05"
                     /product="hypothetical protein"
                     /protein_id="AXY99637.1"
                     /translation="MSLNRIKPIGRLIKYLSGALYDFNRFFKYGGWKINLKNKDERNY
                     YVVKIYHSLEKSMSFTNHNVNSGWTNAKLLTIALEAAVTYQNIGFHDKIGYLVLLEFL
                     DKNKSAEDTELKKYILNKINYISDAIRLKDNIEEYGVKNMSSEDLDGSSHIHPEFFFR
                     SRNSVREFSKKEIPDTLLNKAIQLSLKTPSACNRQSWHIYHLTNAYDIRNALKYQSGN
                     KGFSNRISNLLIICSDLKAFNPGGERYQHWIDGGMYAMSLIYSLHSMGISSCCLNWSC
                     QAKQDLQLRKEFPSILKNHTIIMMLAIGYPENNNKICVSPRRPLHEIYTRL"
     CDS             5475..6569
                     /note="orf06"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf06"
                     /product="Polysaccharide pyruvyl transferase"
                     /protein_id="AXY99638.1"
                     /translation="MKIGIITIHNVSNYGAVLQSYALKEVIKDKYTVNIINFNNKHVS
                     KSLDLIRFTPSLHGILGTIKDILRIIPRYRVINKFNNFILDELDLSEYNNKALTSFTT
                     LISGSDQIWNPACISSNCKFRDEYFLSFATPQQKKISYASSCGAYEFNPEEEKVLAAY
                     LNDYTYISSREYESSMYLSRITNKRVNFVLDPTLLLNKEQWINKIKPKKIIKEKFILL
                     YVIKKTNLLKEVVKKIKFELNLKVILVEQGLYFSNIVDKHIRDAGPADFIGLVNDAEY
                     IVTDSFHGTTFSIIFRKNFLSVSPGKNVNRIKSLLETLHLKNRLINNINDLNDVSINT
                     DIDYHKSNIILREEIKKSKDYLFLAIENSK"
     CDS             6569..7681
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99639.1"
                     /translation="MLNIIINATALKSGGALSILKQFIANTNELDIYHIMISIEIYNI
                     LPKKKNIIYHIRDNNNFLKRIYWDSFLLKKYILKKNINADIIISLQNTSINFKNKPQL
                     IYLHQGLFLHERKWSFFKRGEIKYAIYKYIYPFFIFLHTTKETTFVVQTQWMKDSLIN
                     RFNINPNNIHNLKPTVFLPEKSSNNLKFNIENNKFIFYPATNEIYKNHIFLLESFNDF
                     LKKNRKSKLKLVLTLNKKDNKKILYLLKNNDILNENVIFLGALDYEDVINLYNVSHAI
                     MFPSSIESFGLPLIEAAYLGKKIISLDTNFSQEILNNYSGVTFLKKDLSLWSQEFEKI
                     EKTETISYPSYKSEYTSEWNLFFNLIGKLCSKIKYF"
     CDS             7657..8694
                     /note="UDP-N-acetyl-glucosamine 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /gene="fnlA"
                     /product="fnlA"
                     /protein_id="AXY99640.1"
                     /translation="MFKDKILLITGGTGSFGNAVLRRFLNTDIKEIRIFSRDEKKQDD
                     MRKLFKSDKLKFYIGDVRDYQSVANAMRGVDYVYHAAALKQVPSCEFYPLEAVKTNVL
                     GTENVLEAAITFGVKRIVCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLEHTNTTIC
                     CTRYGNVMASRGSVIPLFIRQLMNNEAITITNPEMTRFMMTLDDAVDLVLHAFKNGQN
                     GDIFVQKAPAATINTLVKALLEITKKPDHKVNIIGTRHGEKQFEALCSREEMFVAQEQ
                     GNYYRIPADNRDLNYSKYFEKGEKDLAIVEDYNSHNTQRLDVNGMKELLRKLDFIREI
                     ESGNIIIPEGV"
     CDS             8695..9570
                     /note="dTDP-4-dehydrorhamnose reductase"
                     /codon_start=1
                     /transl_table=11
                     /gene="qnlA"
                     /product="qnlA"
                     /protein_id="AXY99641.1"
                     /translation="MLNIYILGASGMLGYSIFTNLSEYKGFNVFGTVRNIRKKENYFS
                     KFKNKIIENIDISDLSSLEILFQKNKPDIVINCIGLIKQHSISKQYIQAIKVNALLPH
                     ELAHICDNYQAKLIHFSTDCVFDGKSGNYQQTDIPTATDLYGRTKHLGEVTYGKHLTL
                     RTSIIGHELSSSVSLVDWFLNQKDQVNGYTKAIFSGFPTCYIAKLLAEKIIPNKNLNG
                     LYHLSSEPIDKYTLLSNIAAIYHKNIIINPSEELVIDRSLNSTPLRNELDFNSPSWNE
                     LIKYMYEDYNKRYKK"
     CDS             9567..10694
                     /note="UDP-N-acetylglucosamine 2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="qnlB"
                     /product="qnlB"
                     /protein_id="AXY99642.1"
                     /translation="MKKLKIVTIVGTRPEIIRLSRIINKLDNYCEHTLVHTGQNYDYE
                     LNQIFFDDLEIRAPNIFLECAGSSAAETMAQVISKSDKVFAEIKPDAILILGDTNSAL
                     SAISAKRKKIPIFHMEAGNRCFDLRVPEEINRKIVDHISDVNLPYSDIAREYLLREGI
                     SPDFIVKTGSPMDEVLEFYKDKITSSNILKNLNLSAEKYFLVSMHREENVDSKENIRN
                     FVDTLDSLAKKYNFPIIISTHPRTRKKIEELKLSFNPLVHLMKPLGFSDYVHLQTKAK
                     VVLSDSGTITEESSILNFPAINIREAQERPEGFEEGAVMFTGMNTERILQAISILENQ
                     PRGNLRLINKVNDYIAPNVSEKVLRTIISYTDYVNRVIWKK"
     CDS             10757..11908
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99643.1"
                     /translation="MFHELALELKYHGHEITIITPSKDQPSNLYIDKLDDITIWRFKN
                     GPIKDVKKIQRAINETLLSYNAWKAIKHLINKDSFDGIIYYSPSIFWGKLVNKLKKKC
                     QCPSYLILRDLFPQWIVDAGILKQGSLIEQYFRFFERYSYKQASIIGLMSQKNLDYFY
                     QHNKNKGLKCEILRNWANTTQTNIDTNGYISIRESLNLQNKTIFFYGGNIGHAQDMGN
                     LMRLVKKMSAYPDAHFLFLGQGDEVELIKTLSNKWNLNNFSYLPSVDQTKFKLILKEI
                     DIGLFSLASHHSAHNFPGKLLGYMANHIPILGSVNEGNDLQELVNKENAGFIHINGED
                     DNLFESAKKLHLDIELRKKCGNDAYNLLCKEFSVTSIASTLIGRLRSLK"
     CDS             11905..12306
                     /codon_start=1
                     /transl_table=11
                     /gene="wbuC"
                     /product="wbuC"
                     /protein_id="AXY99644.1"
                     /translation="MKYIDNSSLENLYKEAKKSERLRSHLLLHKSHEDKVQRLLIALV
                     KDSYVEPHYHELPHQWEMFIILEGQIEITLYNHSGEIKEKFLAGENTNISIVNLEPNE
                     IHALKCISQKALMLEVKEGPFDPNYAKKFLI"
     CDS             12367..13533
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99645.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFLATKELDFTATLDKEFAYKDADFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESYGLNSKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLNEFKAISDVIITNRMSSVLNDVEEKVYTRDLFGND"
     CDS             13545..14048
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99646.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(14124..14681)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXY99647.1"
                     /translation="MALTEVTFSELKACLHAEYIGDNKPFSWIRLFHRVFFCQRSRYL
                     FWWRVAQFFYFSKNHFLKKLGIYIGAKNNRKYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKDGKADIRVGNHVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYLTKVVAEIKQLSM"
     CDS             complement(14681..15514)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99648.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDDSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWGQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHAVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(15618..16637)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99649.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLLWGHDAEHVAALEQ
                     ARCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDAR
                     VVWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNT
                     FSEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALIT
                     RGLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQE
                     KIGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEI
                     DSTLF"
     gene            16637..16771
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     6004 a   2652 c   2516 g   5600 t
ORIGIN
        1 tacgaaactc cattaacacg cttacaatta gccagtgcat tgttacgtcg ccgcaacgga
       61 gaaagtaaag aattagaacg cattgaaact gagactcaac gccttgatgg gatgattaat
      121 gacttattgg tgctttcacg taatcaatat aaaaatgagt tgttacgaga aactgtaaaa
      181 gcaaatgagt tgtgggatga tattttagat aacgctaaat ttgaggctga acagagtaat
      241 aagacattac aagtgacaac acctccaggt gcatggccta tttactgtaa cccttactca
      301 ctcgctagcg catttgaaaa tattgttcgt aatgccttac gttattcaaa ttctcgcatt
      361 gaagttgcat tcacagagca aaatcaaggc atcaccatta ttgttgatga cgatggccca
      421 ggtgttagcc ctgaagacag agagcatatc ttccgaccgt tttatcgcac tgacgaagca
      481 agagatcgcg aatcaggtgg tacaggctta ggattagcga ttgttgagac agcagtaagt
      541 caacatcgag gccatgttaa agccgatgac agcccattag gtgggctgag agtagagatt
      601 tggttgccga ggggaagtgc cggtaataaa tagccaatag cctagataga gtaattaaac
      661 tgttgtatca aaatatcctg ccatctattg aagaataggt ggcatattct ttttgttagc
      721 atctaatgat gcaattcaga atgatctggc tgaatcgacc gctattccga tatttatata
      781 tctacaatca atgctaaaat acccagctat tacttgaccg tctggtttga agagtctact
      841 ctcagtgcgg tttttcgcat attgttaaaa tgtgcttata taacaaaact cttatattac
      901 aaaaagataa gggtttttcg agataagttt tggcgctctg ggggccgtaa acccagtgcg
      961 gtagctttag ttaaagtaat taaattcatc ataataatat taaaaattga ttaaataaat
     1021 tatgttctat tcaattcatt acataagagg aatctcagct ttattagttg ttttattcca
     1081 cttaagacct agcattaata atgtatatgc acaaaaaaac ttaggcgatt tactatttct
     1141 tagaggcgat ataggggtag atgctttttt tattattagt ggattcatta tttgtcatgc
     1201 aacaaggaat gataaatcta taatatcatt ttcacttaaa cgttttttta gaatttatcc
     1261 aacatattta ttattttcaa ttcttttcct ttgcataaat ctaattctct ttaatcatct
     1321 tacaataaaa agtttttttg atataatact gattcgccct ttaaattata gtatgggtcc
     1381 accattctat ggctatactt atcttgtagt tggatggtct ttagtatatg agatatgttt
     1441 ttacattata tatctagtat caatgtcact atcccataaa tatagaaata tcatttgctc
     1501 acttatttta tttttattat gttatgcttt acaaaaatat catttaaata catttgatat
     1561 atcagccaca tcaggattaa aagataaaaa tctcggaatt ttaaatattt tatcttcatc
     1621 tatgatgctg aattttattg ttggaatatt tatatatgaa ataacaaagt atataaaagg
     1681 aaatcttcac tttataaata aagaatttat aaaaattatt tttttctttg ttatcactat
     1741 atctatttat tttttcatga taggattcaa ctcttggcac ggtttaaata aatcagtttt
     1801 atttatagca ccactgatct tatcactcat tatttatgaa tataaatgcg gattgaagag
     1861 aatacccgca tttatatttt taggtaatat ttcatattcc ttatatttat cacatcttgt
     1921 tgttacttat gctataaaaa aattcggcgg tcctctttat aatgctaata acggttttag
     1981 tactattttt ataataatct cattatcatt aataatctct tatatttcat ataatacaat
     2041 tgagaaaata tcctctgaag catgtagaaa gataatcatg aaaataaaaa actccgacca
     2101 agaaaataca taaatattga gaaaataaaa atgaatataa aaccaaaaat aaatgcaata
     2161 tacttttttt tatttggttt cttatactat attgtaagcc cagtgatttc attatacttc
     2221 tttgataaaa gctggtttgt attaattgca aaacaacatg taaaactaaa tgactatggg
     2281 ttagcatatt cttttatatc catattatgc ctacttattt tttctcttgc atatattatt
     2341 tttagtaatt taaaactcct aaaaactaac attggtgaaa aagaaaaaga acataaattg
     2401 cttcctttaa taatctttat tattatactt tcacttcttt tatttacaat aataaaatca
     2461 tattattctg gcgtgagttt attctctgga tataatgaag gttataatat ttcattatta
     2521 ggtcctctag caacaatttc cttttcctct attattttta tgttttattt tgaaaaaagg
     2581 aaatataaaa caggattctt tattatatat ttaatatcta atgtattttt acttggcatg
     2641 ggttcaagaa tgtttttttt gataggacta attagtattg caataaatga atataataga
     2701 aatcctaaaa taataaaaac tttaaggttt catattttat ctatctcttt attcttattt
     2761 attttattta ttggaatatg gcgaagtaac tcggagctta gtctagagaa attacttgga
     2821 atattttttg ctgaaccact tttcacatct ataagtgcaa ttaattactt acatattata
     2881 gaaaatgaat ctctaataaa aatcccctgg gatgttattg catcatttct taatttcatt
     2941 ccatctgaat tatttaaaga taaaatagta ataatttcag aaatttctta tgatataaaa
     3001 tcttatagcc cattcggagc aagtagttta ttaacaaata tatatatcaa ctttggaatc
     3061 ttatttccta tatatatctt ttccatagga atggtatttg gaatattaag caagttatct
     3121 tataataaat taatatactc catatatata acaacattac ctttattaat gttccatttt
     3181 tttagagaag gatttataac atatattaaa atacagtttt tcaatggtct aatatttcca
     3241 atatttatca tcttattaat ctctttttta ttgagagtta aacgatgatt aaatcaatat
     3301 tattactttt aagcagttca ctactcagtt cattattaac atttataaca caagcgtaca
     3361 tagcaaatca acttaatcca aatgattttg gtgaattcag ctcaagttta gccttagtaa
     3421 tgctgttagt tccattcgtt gctatggggg ctgatagtta tttattaaaa atatatgctg
     3481 ataaaaaagg taatacttct gaatataatt cattttggat agcctatttt atctttacac
     3541 ttatactctc aattttaatt tatactattt ttaatactac tagtagtagt tcaataattt
     3601 tagtttcaat tttagccagt caagctctaa taaactatat ttcagcaata tatcaaacaa
     3661 aatctaaata taaaaagtta tcattaatac aagccttaca aagtttcttt agatttttca
     3721 ctttaataat tctatcttat gcttttgagt taaacataaa tctggtttat tactcatatt
     3781 tttttgtatc aattctaatt ataattatat gcattatatt aataattaaa gaatctaatt
     3841 tcattgcgat aaagaatatt aaaaatataa aattatattt ctttcaagct attccttttg
     3901 gattaacgcc attgctacat ctaatctatt ttcaaagtga tattattcta attgatagat
     3961 tatactcatc cacagaagca ggtttctata gtgcagcatt tatgttaatt actgcttcat
     4021 atattctccc agcagttata tatcaaaaat atttattacc atatattcat atacttaatg
     4081 caaacaatga tattactaat gaatttaaga tattcaaaac aggtgctttt actatattac
     4141 caattagtat aataatattt ttaatttatt attttttatc cccttatatt gttttagtaa
     4201 tttttggaga agcgtataaa aaaactcaag aaattatgct tattctttca gtatgtataa
     4261 tttttagata tttatcatca catgttggct tatttttgac tgctggtaat ttaataaaag
     4321 taaaaaataa aaatatgcta ctatgtgcaa ttatcaatat tttacttaat gtgatattta
     4381 tcccatcatt tggatcaata gctgctgcat acattacagt gataacagaa atacttttat
     4441 tattaatgtt tttttatagt ttttatatat ttaaaattaa gaggttttat gtcattaaat
     4501 agaattaaac caataggacg ccttataaaa tacttatcag gtgctttata tgattttaat
     4561 agatttttca aatatggtgg ttggaaaata aatcttaaaa ataaagatga aagaaattat
     4621 tatgtagtca aaatttatca tagtttagaa aaaagcatga gtttcactaa tcataacgta
     4681 aattcaggct ggacaaatgc taaactatta actattgctt tagaagctgc agtaacctac
     4741 caaaatattg gttttcatga taaaataggt tacttggttt tactagaatt cttagataaa
     4801 aataaatcag ctgaagatac tgaattaaaa aaatatattc taaataaaat aaattatatt
     4861 tctgatgcta ttagattaaa agataatatt gaagaatatg gtgtgaaaaa catgtcatct
     4921 gaagatcttg atggctccag ccatattcat ccagagtttt ttttcagaag tagaaattcc
     4981 gttcgtgaat tttcaaagaa agaaatacct gatactctcc taaataaagc aatacaatta
     5041 tctttaaaaa caccatctgc ttgtaatagg caatcatggc atatatatca tttaactaat
     5101 gcatatgata taagaaatgc attaaaatat caaagtggta ataaaggctt tagtaataga
     5161 attagcaatt tattaattat ttgtagtgat ttaaaagcat tcaatccagg aggcgagcgc
     5221 tatcaacatt ggattgatgg aggaatgtat gcaatgtcat taatatattc tttgcattcc
     5281 atgggaattt catcttgttg tttaaattgg agctgccaag ccaaacaaga tttacaatta
     5341 agaaaagaat tcccttcgat attaaaaaat catacaatta ttatgatgct agccattgga
     5401 tatcctgaaa ataataataa aatctgtgtt tctccacgtc gcccactaca tgaaatttat
     5461 acaaggttgt aaaaatgaaa attggtatta tcacaattca taatgtaagt aattatggtg
     5521 ctgtattaca atcttacgca ttaaaagaag taataaaaga taaatatact gtaaatatta
     5581 ttaattttaa taataaacat gtatctaaaa gtcttgattt aatcagattt acaccttcac
     5641 ttcatggaat acttggtacc ataaaagata ttttaagaat aataccaaga tatagagtaa
     5701 tcaataaatt taataatttt atcttagatg aattagatct atctgaatat aataataagg
     5761 cattaacttc atttacaact cttatatcgg gcagtgatca aatatggaac cctgcatgta
     5821 tttcatcaaa ttgtaaattt cgtgatgaat attttttatc attcgctaca ccacagcaaa
     5881 aaaagatatc ttatgcctct agttgtgggg cttatgaatt caaccccgaa gaagaaaaag
     5941 tattagccgc gtatctaaac gattatactt acattagttc aagagaatat gaatctagta
     6001 tgtatttatc aagaattaca aataaaagag taaattttgt tcttgatcca acattacttt
     6061 taaataaaga acaatggatt aataaaataa aacctaaaaa aataattaaa gaaaaattta
     6121 ttcttctcta tgttataaag aaaacaaact tattaaaaga ggttgtaaaa aaaattaaat
     6181 tcgaattaaa cttaaaagtg atattagttg agcaaggctt atattttagt aatattgttg
     6241 ataaacatat aagagatgct gggcctgcgg attttattgg cctagtaaat gatgcagaat
     6301 atattgttac agattctttt catggaacaa ctttttctat tatttttaga aaaaatttct
     6361 tatctgtatc accaggtaaa aatgtaaatc gcataaaaag tttacttgaa acattacact
     6421 taaaaaacag gctaataaac aatattaatg atttaaatga tgtatccatt aatactgata
     6481 tcgattatca taaatctaat attatattaa gagaagaaat aaaaaaatct aaggattatt
     6541 tatttttagc tatagagaac tcaaaataat gttaaatatt attattaatg caacagccct
     6601 taaaagtggg ggcgcattat caatattaaa acaatttatt gcaaatacta atgagttaga
     6661 tatatatcat ataatgatat cgatagaaat atataatatt ttacctaaaa agaaaaacat
     6721 tatatatcat atcagagaca ataataattt cttaaaaaga atatattggg attcattctt
     6781 acttaaaaaa tatattttaa agaaaaatat aaatgctgat ataatcatat cattacaaaa
     6841 tacatctata aacttcaaga ataaacctca attaatttat ttgcatcaag gtttattttt
     6901 acatgaaaga aaatggtctt tttttaagag aggagaaatc aaatatgcta tatataaata
     6961 catatatcct tttttcatat ttttacatac cactaaagaa actacttttg tagtacaaac
     7021 acaatggatg aaagattctt tgattaatag gtttaatatt aatcccaaca atattcacaa
     7081 tttaaaacca acagtttttc ttccagaaaa atcaagcaat aatttaaaat tcaatataga
     7141 aaataataaa tttatttttt atccggccac aaatgaaatt tataaaaatc atatattttt
     7201 actagagtca tttaatgatt ttttaaagaa gaatagaaag tcaaaattaa aattagttct
     7261 tacattaaat aaaaaagata ataaaaaaat actctattta ttaaaaaaca atgatatatt
     7321 gaatgaaaat gttatttttt taggtgcatt agattatgaa gatgtaataa atttatataa
     7381 tgtctcacat gctataatgt ttccaagttc tatagaaagt tttggtcttc cgcttattga
     7441 agccgcttac ctaggtaaaa aaattatttc tcttgatact aatttttcac aagaaatact
     7501 aaataattat tcaggtgtta cttttttaaa aaaagactta tctctttggt ctcaagaatt
     7561 tgaaaaaata gaaaaaacag aaacaatatc atatccatca tataaatcag aatatacatc
     7621 cgaatggaat cttttcttta atcttatagg caaattatgt tcaaagataa aatactttta
     7681 atcacaggtg gtactggttc ttttggtaat gctgtattac gtcgtttttt aaatactgat
     7741 ataaaagaaa ttcgtatatt ttctcgtgat gagaaaaaac aagatgatat gcgaaaatta
     7801 tttaaatcag ataaattgaa attctatatt ggtgatgtca gagattatca aagtgttgcc
     7861 aatgctatgc gtggtgtaga ttatgtgtat catgcagcag cattaaaaca agtaccttcc
     7921 tgtgaattct atccattaga agcagtaaaa actaatgtat tagggacaga aaatgtatta
     7981 gaagcagcaa tcacatttgg ggttaaacgt attgtttgtt taagtacaga taaagccgtt
     8041 tacccaataa atgcgatggg catatcaaaa gcaatgatgg aaaaagtcat tgttgctaaa
     8101 agtagaaatc tagaacacac caataccaca atttgctgta ctagatatgg aaatgtaatg
     8161 gcatctagag gctccgttat cccattattt attcgacaac ttatgaataa tgaagcaatc
     8221 actatcacta atccagaaat gactcgtttt atgatgacat tagatgatgc agtagacctc
     8281 gtgttacatg catttaagaa tggtcaaaat ggtgatattt ttgtccaaaa agcacctgca
     8341 gcgacaatta atactctagt caaagcattg ttagaaataa caaaaaagcc agatcacaaa
     8401 gttaatatta ttggtactcg tcacggtgaa aaacaatttg aagctttatg cagccgagaa
     8461 gaaatgttcg ttgctcaaga acaaggtaac tattatcgaa ttccagctga taatcgagat
     8521 ttaaattatt caaaatactt tgaaaaaggc gaaaaggatt tagctattgt tgaagattat
     8581 aattcacata atactcaacg tttagacgtt aacggtatga aagagcttct tagaaaactt
     8641 gattttatta gagaaattga gtctggaaac atcattattc ctgaaggtgt ttaaatgtta
     8701 aatatttata ttttaggtgc cagtggtatg ctgggttata gtatttttac caatttatct
     8761 gaatataaag gttttaatgt ttttggcaca gttagaaata taagaaaaaa agaaaattat
     8821 ttttctaaat ttaaaaacaa aattatagaa aatattgata tctctgattt atcttctctt
     8881 gaaattttat ttcaaaaaaa caaaccagac attgtaatta actgcattgg attaataaaa
     8941 caacatagta tttctaaaca atatattcag gcgataaaag taaatgcttt attacctcac
     9001 gaattagctc atatttgtga caattatcaa gcaaagttaa ttcacttttc tacagactgt
     9061 gtctttgatg ggaagtcagg taactatcaa caaactgata tccctacagc aacagatctt
     9121 tacggccgaa ctaaacattt aggtgaagtc acttatggaa agcatttaac tttacgaaca
     9181 tcaattatag gtcacgaatt atcttcaagc gttagcctag tagattggtt tttaaatcag
     9241 aaagatcagg taaatggata tactaaagcc attttttctg gttttcctac atgttatata
     9301 gcaaagttgc tagctgaaaa aataataccc aataaaaatt taaatggttt atatcattta
     9361 tcaagtgaac caattgataa atatacacta ttatcaaata ttgcggctat ttatcataaa
     9421 aatattatta taaatccatc agaagagctt gttatagatc gttcacttaa ttccacacct
     9481 ttacgcaacg agcttgattt taattcacca tcatggaatg aattaatcaa atacatgtat
     9541 gaagactata acaaaaggta taaaaagtga aaaaattaaa aattgtaact atagtaggaa
     9601 cacgtccaga aataattcga ctatcacgaa taataaataa gttagataat tattgtgaac
     9661 atactcttgt tcatactgga cagaactatg attatgaatt aaatcaaata ttctttgatg
     9721 acctcgaaat cagagctccc aatatttttc ttgaatgtgc tggttcatct gctgctgaaa
     9781 ctatggctca agtaatcagc aagtctgata aagtgtttgc tgaaattaaa cctgatgcaa
     9841 tattaatatt aggtgataca aatagtgccc tttccgcaat ttctgctaaa agaaagaaaa
     9901 tccctatatt tcatatggaa gcaggtaatc gttgttttga ccttagagta ccagaagaaa
     9961 taaataggaa aatagttgac catatctctg atgtcaacct tccttatagc gatatagcaa
    10021 gagaatactt acttcgagaa ggaattagtc ctgattttat agtaaaaaca ggaagcccta
    10081 tggatgaagt attagagttc tataaagata aaattacatc ctctaacata ttaaaaaatc
    10141 taaatttgtc tgcagaaaaa tatttcttag tcagtatgca tagagaagaa aatgttgact
    10201 caaaagagaa tataagaaat ttcgttgata ctttagattc tctcgcaaaa aaatacaact
    10261 tcccaattat catatccaca cacccaagaa caagaaaaaa aatagaagag ttaaagctat
    10321 catttaatcc attagttcat ttaatgaagc ctctaggttt ttctgattat gtacatctgc
    10381 aaactaaagc taaagttgtc ttaagtgata gtgggactat tactgaagaa tcttcaattt
    10441 taaactttcc tgccattaat ataagagagg ctcaggaaag acccgaagga tttgaagaag
    10501 gtgctgtgat gttcacagga atgaatacag aaagaatttt acaagctata tccatacttg
    10561 agaaccaacc tagaggaaac ttacgtctaa ttaataaagt taatgattat attgcaccaa
    10621 atgtatctga aaaagtacta aggactatca ttagctatac agattatgtt aatcgagtaa
    10681 tatggaaaaa gtaaaactaa agattgcttt aatcattgac gattacttgc cccatagcac
    10741 tcgagttggg gcaaagatgt ttcatgaatt agcactagaa ttaaaatatc atggtcatga
    10801 gattaccatt attacaccgt caaaagatca accatctaat ctatatattg ataaattaga
    10861 tgatattact atctggcgtt ttaaaaatgg tcctattaag gatgtaaaaa agatccaaag
    10921 agcaattaat gaaacactac tttcttacaa tgcttggaaa gctattaaac atttaatcaa
    10981 taaggattct tttgatggca taatttacta ttctccttct atattttggg gaaaattagt
    11041 taataaacta aagaaaaaat gccagtgtcc ttcttattta atattaaggg atttatttcc
    11101 tcaatggatt gttgatgctg gtattttaaa gcaaggttca ttgatcgaac agtactttcg
    11161 attttttgaa cgttactcct ataaacaagc atcaataatt ggcttaatgt ctcaaaaaaa
    11221 tctagactat ttttatcaac ataataaaaa taaaggtttg aaatgtgaaa ttttaagaaa
    11281 ttgggcaaat actactcaaa caaacattga tactaatgga tatatttcaa tacgtgaatc
    11341 tttaaactta caaaataaaa ctattttctt ttatggtgga aacattggtc atgcccaaga
    11401 tatgggtaat ttgatgcgac tagtgaagaa aatgagtgct tatcctgatg ctcattttct
    11461 ctttcttgga caaggcgatg aagttgaatt aattaaaact ttatctaata aatggaattt
    11521 aaataatttt agctatctac cttcagttga tcagactaaa tttaaattaa ttcttaaaga
    11581 aattgatatt ggtttatttt cattagcgtc acatcactct gcacataatt ttccaggtaa
    11641 attattagga tatatggcta atcatatccc tatattaggt agcgttaatg aaggaaacga
    11701 cttacaagaa ctagtaaata aagaaaatgc aggttttatt catataaatg gcgaagacga
    11761 taatttattt gaatctgcta aaaagctcca ccttgatata gaactacgaa aaaaatgtgg
    11821 aaatgatgct tataatctgt tgtgtaaaga gttttcagtt acttctattg catcgacact
    11881 aataggtagg ttgagatctt taaaatgaaa tatattgata attcatcgtt agagaattta
    11941 tataaagaag caaaaaaatc agaaaggtta cgttctcatt tattattaca taaatcccat
    12001 gaagacaaag tacaacgctt acttattgct ttagtaaaag atagctatgt tgaaccacat
    12061 tatcacgagt tacctcatca atgggaaatg tttattattt tggaagggca aattgaaatt
    12121 acactatata accattctgg agaaataaaa gaaaaattct tagctggaga aaatactaat
    12181 atttcgattg taaacttaga accaaatgaa atacacgctc ttaaatgcat ctctcaaaaa
    12241 gcattaatgt tggaagtaaa agaggggcct tttgatccca attatgctaa aaaattctta
    12301 atttaataga tcaatttact atctataata cacctataaa gttaggttaa tcttaaaggt
    12361 ctatttatga aaatcaccat ctcaggtacc ggctatgtcg gtctatctaa cggaatactc
    12421 ctttcccaaa acaacaaagt tgttgcgtta gatatcaata aagaaaaagt tgaattactg
    12481 aacaaaaaaa tctctccaat tacagataaa gaaattgaag aatttttagc aaccaaagag
    12541 ctcgatttta ctgcaacctt agataaagag ttcgcttata aagatgcgga ttttgttatc
    12601 attgctacac caactgatta tgatcctaaa acaaattact ttaatacctc ctctgtagag
    12661 tctgttatcc gtgatgtttt agaatataat ccaaacacca caatgattat aaaatcaacc
    12721 attcctgttg gttttacgaa agaaatgaga gaaaaatata atacggataa tattattttc
    12781 tctcctgaat tcttacgtga aggtcgtgca ctttacgata atttataccc atctcgtatt
    12841 gttattggtg aacaatcaga aagagcacaa aaatttgcag accttttaca acaaggtgca
    12901 ttaaagaaag atattgatgt tctgttcact gactcaacag aagcagaagc cattaaatta
    12961 tttgccaata cttatttagc gctacgtgtt gcttacttta atgaacttga tagttatgct
    13021 gaatcttatg gtttgaactc aaaacaaatc atcgaaggcg tctgcttaga tcctcgtatc
    13081 ggcaaccatt ataataaccc atcattcggt tatggcggtt actgcttacc aaaagatact
    13141 aaacagctgt tagcaaacta cgactcggtg cctaataata ttattagtgc aatcgtcgaa
    13201 gctaaccgca cacgtaaaga ctttattgcc gactcaatta tcgctaaatc accaaaaatc
    13261 gtaggtgttt atcgtttaat tatgaaatct ggctctgata atttccgttc ttcttctatc
    13321 caaggcatta tgaaacgcat taaagccaaa gggattgagg tggtaatcta tgagccagag
    13381 atgaaagaag agactttctt taactcaaaa ttaattcgtg atttaaatga gtttaaagca
    13441 atctctgatg tcattattac taatagaatg tcatctgtac taaacgacgt tgaagaaaaa
    13501 gtttatactc gtgacctctt tggtaacgac taaatcgatt ttatatgctc aatatcgtct
    13561 tatttgaacc cgaaattcca ccgaatacgg gtaatattat ccgcctgtgt gctaacacag
    13621 gctttcatct tcatttgatc caacctttgg gttttacttg ggatgataaa cgtcttcgtc
    13681 gtgcaggact tgattatcgt gaatttgctg atattaaaca gcatcacgat tactatgctt
    13741 ttttagaaag tgaaggttta aaccctgata atgcacaatc accaacgggt gcgcgtgtat
    13801 ttgcgttaac gacaaaaggt acaccagcac atagcaatgt aagctatcaa gatggagatt
    13861 atctcctttt tggccctgaa acccgtggtt tgccacctta tgtgttagat aatatgccaa
    13921 cacagcagaa aatcagagtt ccaatgcttg ctgatagtcg cagtatgaac ctgtcaaata
    13981 ccgttgcagt tgtggttttt gaagcatggc gtcaacttgg ttatcctggt gcgttattaa
    14041 gagactgaaa tgcaaaaagc gctgatcata aataatgagc aagcgctttt ttaatttcaa
    14101 atgactaaaa acttaattta ttattacatt gagagttgtt ttatttcagc aacaaccttc
    14161 gtgagatacc tacacccttc ccctacatcc ttcaatacca atgcgtgagc accaatcact
    14221 gcattatcac caatctctat ctcaccgccg ataatcgaag aattacaacc aatataaacg
    14281 tggttgccga ctcgaatatc tgctttacca tccttttctt ttactccgat ggtcacacct
    14341 tgcattaacg tcacattctc acctaattga cagtaattag tcagcacaat accgatagga
    14401 tgtggtaatg aaagcccttt tcctatgcgc gttcttaatg agatatcatt gcaatattta
    14461 cgattgtttt tagcaccaat gtaaatgcct aattttttga gaaaatgatt tttagagaaa
    14521 taaaaaaatt gagcgacacg ccaccaaaac agataacgtg aacgttggca gaaaaagacg
    14581 cgatgaaaaa gtcttatcca actaaaaggc ttgttatcac cgatatattc agcgtgtaaa
    14641 caagccttaa gttcagaaaa tgtaacttct gttaatgcca ttaaatgcca tcaccacatt
    14701 caaaaccagg tactacacca ttaaagtgtt gatccatatc gagtgaaggt ttatcactcg
    14761 tcggcttacc aacaatgcga gcaggaaccc ctgcaaccgt ggtatgtgca ggcactgcat
    14821 gtaatacgac agatcccgca ccaattttag caccgcgacc aatctcaata ttgccgagta
    14881 ttttagcacc cgcaccaatc atcacacctt cacgcacttt aggatgacga tcaccacagg
    14941 ttttacctgt tccgcctaaa gtcacagatt gtaaaataga aacatcattc tcaacaacag
    15001 ctgtctcacc aattacaatg cctgttgcgt gatcaagcat aataccgtgg ccaatacgtg
    15061 cagctgggtg aatatcgaca ccaaacgtaa cagaaatctg gttttgtagg taaacagcaa
    15121 gcgctttgcg atcttgtccc cataaccaat gtgctatgcg ataagcctgc aacgcatgaa
    15181 agccttttaa ataaagcaac ggtgtggagt atttatcaac tgcggggtcg cgtaaacgaa
    15241 cagctgaaat atcataagcc gcagattcga tcatagaatc atcgctacga tacgcttctt
    15301 caacaatctc acgaacggca atcgcaggca ttgtctgtgt cgccagctta ttcgctagca
    15361 tataacttaa ggcgctacct aagttttcat gtttgagtaa tgtcgcatgg aagaaactcg
    15421 ccaaaatcgg ctcgcaatca gccaaacatc ttgcttcatt tttaatatga tcccagactc
    15481 tttttagttc ttctagcgac atactcactc tcattctttt tacattgaaa cctaagtaac
    15541 aacttaagct tcttatgttg aaaaccgaaa cagacacctt aaaccaatgc gttaagtagc
    15601 gattatttaa aatagactta gaacagagtg ctgtctattt catcttttgt tgcgcgacct
    15661 aataaagata gtgccgcctc tttcgcgtct ttatgttggt ataaaatttg gtagatctgc
    15721 tcagtgatag gcatttcaac accgacttgt tctgctaagg cgcgaacttc tttagtgttc
    15781 cgataacctt caaccacctg cccgattttc tcttgggcag tatcaacatc aagcccttgg
    15841 cctaacatca taccaaatcg gcgattacgg gattggttat cggtacaagt taaaacgaga
    15901 tcgcctaacc cggccattcc cataaacgtc gcagcatctg cacctaatgc tttacctaaa
    15961 cggctcattt cagcaagtcc gcgggtaatt agtgctgtac gtgcattcgc gccaaacccc
    16021 ataccatcag acattccagc accaatagca atcacgtttt ttaccgcgcc acctaattgc
    16081 acaccgatga agtcaggatt tttatacaca cggaaacttt taccacaatg gaacaactgt
    16141 tgaagctctt cagaaaacgt attatctgtt gatgcaacag atatcgcagt aggcagacct
    16201 gctgcaagct ctttggcaaa agtaggacca gataaaactg cgagcgggat ttcattacct
    16261 aatacttcac gcgccacatc ttgtaacaag cgaccagtat gagcctcaag tccttttgtc
    16321 gcccaaacaa cacgtgcatc ctgacgtaaa aagggtttga tctgctgtaa tacgtcacca
    16381 aaaacatggc ttgggatcac aaccagaata ttacggctag cttcaatggc tttttgcaaa
    16441 gaagcttcca tgtataaact atcaggaaaa gaaacatcgg gcagaaatgc ctgattacaa
    16501 cgtgcttgtt ctaacgccgc aacatgttca gcatcatgac cccaaagtag tacaacatca
    16561 tggccattgc gtgctaaggt aatcgctaaa gcggtgccgt atgaaccggc accgataact
    16621 gtcatagaag cgttcattaa gcttcttcag ccccttcatt agtagcatca gtttgttgct
    16681 gttgtaaata gttcataaac aacgcatcaa agttaactgg tgctaagttc agttgtggga
    16741 aagtaccacg gctgactaag ttagtgatac at
//
