LOCUS       KY710707               14167 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 57/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710707
VERSION     KY710707.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 14167)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14167)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14167
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 57/57"
                     /serotype="O32"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..14167
                     /note="O antigen gene cluster"
     gene            2..478
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             890..1786
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlD"
                     /product="rmlD"
                     /protein_id="AXY99650.1"
                     /translation="MKIILFGKNGQVGWELQRSLAPLGTVIALDSKSIEYKADFLFPS
                     ELEKTILSIKPDIIVNAAAYTNVDNAETDNINAFKVNCESVEVIAKTAEKIGALLIHY
                     STDYVFDGTNDTPWLETDTPAPLNIYGKSKLAGEQAIINNCKKYLIFRTSWVYASRGN
                     NFAKTMLKLGKKHDSLSVINDQYGAPTSAELLADCTAHAIKSTLDNEEPLYGLYHLTA
                     KGTTTWFEYASLIFKYAREYSLPLVVKNLTPIPATEYPLPASRPYNSRLNTQKFEKNF
                     RLELPNWKFGIYRMLNEISDNK"
     CDS             1812..2684
                     /note="glucose-1-phosphate-thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlA"
                     /product="rmlA"
                     /protein_id="AXY99651.1"
                     /translation="MKKYKGIILAGGSGTRLYPVTMSVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGIYLQYKIQPSPDGLAQAFILGEDFIGS
                     DNCALVLGDNIFYGHDLPKQLEQAIQKDQGATVFAYHVNDPERYGVVEFDQNKVAISL
                     EEKPLKPKSNYAVTGLYFYDNDVINIAKNLQPSPRGELEITDVNKVYLEQHKLSVAIM
                     GRGYAWLDTGTHKSLIEAGNFIQTLEERQGLKVSCPEEIAYRKGFINKEQVEILAKPL
                     SKNDYGKYLLKMTK"
     CDS             2693..3235
                     /note="orf04"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf04"
                     /product="maltose O-acetyltransferase"
                     /protein_id="AXY99652.1"
                     /translation="MNILKKIINIIEKKFFYYKYLKKIGVKLGNNCLINKQTNFGSEP
                     YLINIGNNFYCSSNIQFVTHDGSINVIRNLYPEFKKHDLISPINIGNNVFLGINVIVL
                     PGTNIEDNIIVGAGSIVKGHLISGHVYAGIPAKKICTLNEFKEKNKSVFIETKNLSYQ
                     RKKKFLLKHFKNINSSRKRK"
     CDS             3238..3783
                     /note="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlC"
                     /product="rmlC"
                     /protein_id="AXY99653.1"
                     /translation="MKILDTNIPDIKIIEPTVYGDHRGFFMEVWNQKKFEELVTKTHT
                     SFVQDNHSKSCKGILRGLHYQSKNTQGKLVRVVKGEVFDVAVDIRKNSKTFGKWVGVY
                     LSDENKRQLWIPEGFAHGFYVTSEEAEFTYKCTDYYNPSAEHSILWDDPEINISWPIT
                     NYPILSEKDKTGIKLKDSILL"
     CDS             3785..5035
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99654.1"
                     /translation="MYYLKKLITNNLQTVKNITYLSLIQFLTIILPFITYPYFIKTLG
                     FSNYGKVIFYQTIITYISMIINYGFNFSGVRAITTAKTVSEESEIITSIYLIKFIIFL
                     LVLIIISLLFFFIKNIDILLLLASLSLVFNELLFPQWYFQAKEKLKIITILTFAVRCL
                     SLVLMFILITEKADYYLVPLLNGIGFFIIGVISFFLILKDGTKLKFPDFNLIFNLFKE
                     STHYFLTSLIISIKNKTDLIIIGIFINKEMVVAYDLALKIHTMSLMPINIINNAVFSK
                     MSKQRNKKFLIQLSIFTFLISLIGIALVINILPFIVNILSDGQKSQLVIKLATILILS
                     LPFFSISTTLSQNGIIVFGYSKYNLYGAISTTIIYLLGILLLFILELQNNIYSYAYLT
                     VFIFLYEMSYRLILCKRIGLLNFK"
     CDS             5072..6154
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99655.1"
                     /translation="MYYSTLFFYITIYILCILINFLLKSNKKLSVFYLSLFLATAFGL
                     RDYVGNDYEQYVNIYNHINIFNDYDKIELGYFYLNKLSYYIFGEQGYYFLFFICSFLT
                     YYFIFSIAYQNNILTTSISLLFFSGFVFFSNNAIRQALVISIFIYNLKNLEKNHYKYL
                     IITILSSTLIHFSALLFLILFLFPKKKNIPTIFLCILIPFIFTLYQFDIVNLIIKKII
                     IYIPKYGELYYNRVIEFESKEKGSGLVIIFYYMLLIYLIYNRNKIKTQYSNIFIIGYI
                     LFLLAPQVEMWERIMIPFYYTNILFIPLIIKNIKKKHSPNKILGYVFIILLSFLFIYQ
                     TIENKNKNGISPFNHLFLKQVGIVLE"
     CDS             6163..6948
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99656.1"
                     /translation="MNMNNKRLVYTVILDDYDSLIEIKEKNENIDYICITDNKNITSN
                     TWEIIYIEKEDNDSTIINRMYKMLPHRFFPHYQTLYIDGNIAIIKDINNLFDKYLTQN
                     SIAAPKHSIRNCIYEESLACLKAKKEKEYNIHLLLMKLHEKKYPFNNGLLENNILFRK
                     NEKKINDVMEDWFVIFKNLCQRDQLSLCYLLWENNIKYQFVKEGPRYERNYFKINFHN
                     KEKKLNILKKIHLYSKLNNKNSKLAFIINKIIETLLQNRKLKK"
     CDS             6945..7838
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99657.1"
                     /translation="MINAIIITYNPDLNILKKNILTLSTSKKINEIIIIDNSDNADNS
                     IEFSKINNTTNRVKFFILKENKGIAFAQNIGLKYSVKSGLEYAILFDQDSFISSDLIN
                     LLLIGIKECNELNIKIAAVGPRPYDILEKRKSKSIIQKENIINKNITVCSQIIASGKL
                     INLSCLNTVGLMDEDLFIDGVDHEWCWRAKRKGYNVAIIENAIMQHTLGDSRGNFLGL
                     TYKIGAPVRLYYQFRNIIILSRRNYVPSYWKMRNLLGMIFRFIIFSCSTNEKKTRRKY
                     MIKGLIDGLKNKKGSVKNSNK"
     CDS             7841..8662
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99658.1"
                     /translation="MKNYHIAVAMSVYKNDNPKFLYESIISILSQEEVNVTLFIQIDG
                     MISDKLKKILILFNEHYNINLFWNKENKGLATRLNQIIDEVIKENKFDYLARMDADDI
                     SHPRRFITQATFLDKHKDISVVGSDVIEISENGEQLFYKKMHSNHDDIVSNVIQKCPF
                     NHPSVMFNIAVFKENFRYKPSLKNTQDYYLWIDLIFAKKRLANINLPLLWFRVDNNFH
                     QRRGIQKSLNESFSRIYAIKKLKIYSVRNILHIPLLFMLRISPEVIKKIAYKKYR"
     CDS             8685..9851
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99659.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNKKISP
                     ITDKEIEEFLATKELDFTATLDKEFAYKDADFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYDLNAKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIISKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLVRDLNEFKAMSDVIITNRKSSALNDVEEKVYTRDLFGND"
     CDS             9877..10887
                     /note="NAD-dependent epimerase/dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gla"
                     /product="gla"
                     /protein_id="AXY99660.1"
                     /translation="MKYLVTGASGFIGFHLIKKLIQQGETVVGIDNLNDYYDVSLKKS
                     RLSILNKLNNFSFALIDLADREKMARLFEAEKFDKVIHLAAQAGVRYSLVNPFSYADS
                     NLTGFLTILEGCRHNDIKHLVYASSSSVYGLNDELPFSPHDQANHPVSLYAATKKANE
                     LMAHSYSHLYGIPTTGLRFFTVYGPWGRPDMALFKFTKAIINDEPIDIYNNGEMKRDF
                     TYVEDIVEGVARIADVIPAPQQDWKVSIGTPANSSAPYKVYNIGNGSPVNLMDYISAL
                     EAHLGKTADKNMLPMQPGDVYTTWADTEDLFKATGYKPQTSVDEGVKQFVDWYKNYYQ
                     VK"
     CDS             10891..11394
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99661.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(11470..12027)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXY99662.1"
                     /translation="MALTEVTFSELKACLHAEYIGDNKPFSWIRLFHRVFFCQRSRYL
                     FWWRVAQFFYFSKNRFLKKLGIYIGAKNNRKYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKDGKADIRVGNHVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYLTKVVAEIKQLSM"
     CDS             complement(12027..12860)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99663.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDDSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWGQDRKALAVYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHAVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(12964..13980)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99664.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDAEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     STLF"
     gene            complement(13980..14165)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5119 a   2273 c   2202 g   4573 t
ORIGIN
        1 aggaccccta cgtgaaaccg taaaagcaaa tgagttgtgg gatgatattt tagataacgc
       61 taaatttgag gctgaacaga gtaataagac attacaagtg acaacacctc caggtgcatg
      121 gcctatttac tgtaaccctt actcactcgc tagcgcattt gaaaatattg ttcgtaatgc
      181 cttacgttat tcaaattctc gcattgaagt tgcattcaca gagcaaaatc aaggcatcac
      241 cattattgtt gatgacgatg gcccaggtgt tagccctgaa gacagagagc atatcttccg
      301 accgttttat cgcactgacg aagcaagaga tcgcgaatca ggtggtacag gcttaggatt
      361 agcgattgtt gagacagcag taagtcaaca tcgaggccat gttaaagccg atgacagccc
      421 attaggtggg ctgagagtag agatttggtt gccgagggga agtgccggta ataaatagcc
      481 aatagcctag atagagtaat taaactgttg tatcaaaata tcctgccatc tattgaagaa
      541 taggtggcat attctttttg ttagcatcta atgatgcaat tcagaatgat ctggctgaat
      601 cgaccgctat tccgatattt atatatctac aatcaatgct aaaatagcca gctattactt
      661 gaccgtctgg tttgaagagt ctactctcag tgcggttttt cgcatattgt taaaatgtgc
      721 ttatataaca aaactcttat attacaaaaa gataagggtt tttcgagata agttttggcg
      781 ctctgggggc cgtaaaccca gtgcggtagc tttgcatgta cgatgatatc gatataacat
      841 cttatattca aatgtatatt atattttatg ggtagttaaa aaataaataa tgaaaattat
      901 cttatttgga aaaaacgggc aagtaggttg ggaattacag cgctctctag ctccattagg
      961 aactgttatt gctttagatt ctaaatcaat agaatataag gctgattttt tattcccttc
     1021 tgaactagaa aaaaccattt tatctattaa gcctgatatt attgtcaatg cagcggccta
     1081 tacaaatgta gataatgctg aaactgataa cattaatgca tttaaagtaa attgtgaaag
     1141 tgttgaggta attgcaaaaa cagctgaaaa aattggagct ttattaatac attattcaac
     1201 tgactatgtt tttgatggaa ctaatgatac tccttggtta gaaacagata ctccagcacc
     1261 tttaaatatt tatggaaaaa gtaaactagc aggcgaacaa gctattatta ataactgtaa
     1321 aaaatatcta atatttagaa ctagttgggt atacgcaagt agaggcaata actttgcaaa
     1381 gacgatgctt aaactaggaa aaaaacatga ctcactatca gtaattaatg atcagtatgg
     1441 agctccgaca agtgctgaat tgttagctga ttgtactgct cacgcaatta aaagtacatt
     1501 agataatgaa gaaccacttt atgggttata ccatttaacc gcaaaaggaa caacaacttg
     1561 gtttgagtat gcatcattaa tatttaaata tgcacgagaa tattcattac ctttagtagt
     1621 taaaaattta actccaattc ctgccacaga atacccttta ccagcatcta gaccatataa
     1681 ctctcgttta aacacccaaa aattcgaaaa aaacttcaga ttagaattac ctaactggaa
     1741 atttggcata tatagaatgc taaacgaaat ttcagataac aaataaacac taattcttac
     1801 gaacttataa aatgaaaaaa tataaaggca ttattcttgc tggcggttca ggaacacgac
     1861 tttatcctgt cacaatgtca gtaagcaaac aattactacc aatttatgat aagccaatga
     1921 tctattatcc tcttagtacc cttatgttag caggaataag agatatatta attattagca
     1981 ctcctcaaga tacacctaga tttcaacaat tgttaggcga tggtagccaa tggggtatct
     2041 accttcaata taaaattcaa cctagtcctg acggcctagc gcaagcattt attcttggtg
     2101 aagattttat tggttcagat aattgtgctc ttgttttagg tgataatatt ttttatggcc
     2161 atgacctccc taaacaatta gaacaagcaa ttcaaaaaga tcaaggtgcg acagtttttg
     2221 cttatcatgt taatgatcct gaacgctatg gagttgtcga attcgatcaa aataaagtag
     2281 ctatttcatt agaagaaaaa ccactaaaac ccaaaagtaa ttatgcagtc actggtttat
     2341 atttctatga taatgatgtc attaatattg ccaagaattt acaaccctcc ccacgaggtg
     2401 aattagaaat cacggatgta aataaagtat atctagaaca gcataagctc tcagttgcca
     2461 taatgggacg tggctacgca tggttagata caggaacaca taaaagcctt attgaagcag
     2521 gtaatttcat tcaaacttta gaagaaagac aaggattgaa agtttcttgt ccagaagaaa
     2581 ttgcttatag aaaaggattt atcaataaag aacaagtaga aatactcgcc aaacctttat
     2641 ctaaaaacga ttatggtaaa tatctattaa aaatgactaa ataagatatc caatgaatat
     2701 tctaaaaaaa atcataaata taatagaaaa aaaatttttt tattacaaat acttaaaaaa
     2761 aataggtgta aaattaggaa ataattgtct tattaataaa caaactaact ttggctctga
     2821 gccgtactta attaatattg gtaataattt ctattgttca tctaatatac aatttgttac
     2881 acatgatggt tccataaatg ttatacgaaa tctttatcct gaatttaaaa aacatgatct
     2941 aatatcgcct attaatatag gcaataatgt ttttttgggt ataaatgtta tagttttacc
     3001 aggaaccaat attgaagata atattatagt tggtgctggt agcatagtaa aaggtcacct
     3061 aatttctggt catgtttatg caggaattcc tgcaaaaaaa atatgtacat tgaatgaatt
     3121 taaagaaaaa aataaatcag tttttataga gacaaaaaat ttaagttatc aaaggaaaaa
     3181 aaagtttcta ttaaaacatt tcaaaaacat caattcttct agaaaaagaa aataaatatg
     3241 aaaattttag atacaaatat tcctgatata aaaataattg aaccaacagt atatggtgat
     3301 catagaggat tctttatgga ggtatggaac caaaaaaagt ttgaagaatt agtcacaaaa
     3361 acacacacat catttgtcca agataatcat tccaaatctt gtaaaggaat tttacgcgga
     3421 ctacactatc aatcaaaaaa tacacagggt aaattagtta gggtcgttaa aggcgaagtt
     3481 tttgatgttg ccgttgatat aagaaaaaat tcaaaaacat tcgggaaatg ggttggagta
     3541 tatttatctg atgaaaataa acgacaacta tggatacctg aaggtttcgc tcatggattt
     3601 tatgtaacaa gtgaagaagc agaatttaca tataagtgca ctgattatta taatccttcc
     3661 gcagaacatt ctatattatg ggatgatcct gaaattaata tatcttggcc tataactaat
     3721 tatccaatac tttcagaaaa agataaaact ggaataaaac taaaagatag tattttatta
     3781 taaaatgtat tacctaaaga aattaataac taataattta caaacagtta aaaatataac
     3841 atatctttct ttaattcaat ttttaacaat catattgcct ttcataactt atccatattt
     3901 tataaaaaca ttaggttttt ctaactatgg gaaagtgatt ttttatcaaa ctattattac
     3961 atacatcagt atgattatta attatggttt taattttagt ggtgttagag caataactac
     4021 agcaaaaaca gtatctgaag aaagtgaaat tataacttct atttacttaa ttaaatttat
     4081 tatttttctt cttgttttaa taataatatc tttattgttc tttttcatta aaaatataga
     4141 tatattactt ctacttgcat cactatctct agtatttaat gaattattat ttcctcagtg
     4201 gtattttcaa gcaaaagaaa aattaaaaat tataacaatt ctcacctttg ctgtaagatg
     4261 tttatcttta gttttaatgt ttattttaat aacagaaaaa gcggattatt atcttgttcc
     4321 tttactaaat ggaatagggt tctttattat aggagtaata tcattctttt taattctaaa
     4381 agatggaacc aaattaaagt tcccagattt caatctaata tttaatctat ttaaagaaag
     4441 tacacactat tttcttacca gtttaattat ttccataaaa aataaaactg atttaattat
     4501 tattggtata tttataaata aagaaatggt tgtcgcatat gatctagcat taaaaataca
     4561 taccatgtcc ttaatgccaa ttaatattat aaataatgcc gttttctcta aaatgtcaaa
     4621 acaaagaaat aaaaaatttt taattcaatt atctattttt acatttttaa tttctttaat
     4681 tggcattgct ttagttataa atatattacc atttatagtt aatatattat cagatggaca
     4741 aaagagtcaa ttagtcatta aattagcaac cattttaatt ttatctttac cttttttttc
     4801 cattagcaca acattgtcac aaaatggaat aatagtgttt ggatatagta aatataatct
     4861 ttatggagct atatccacaa ctatcatata tcttcttggc atcttattat tatttatatt
     4921 agagttacaa aataatatat atagttatgc ttatttaact gtttttatat ttttatatga
     4981 aatgagttat aggcttattt tatgtaaaag aatcgggtta ctaaatttca aatagcaaca
     5041 acataaaaac ttccaataat aaggttttta tatgtattat agtacattgt ttttttatat
     5101 aaccatatat attttatgta tactaataaa tttccttctt aaatctaata aaaaattatc
     5161 tgttttttat ttatcactat tcttagcaac agcttttggc ctacgagatt atgttggaaa
     5221 tgattatgag caatatgtaa atatctataa ccatataaat atatttaatg attatgataa
     5281 aatagaactt ggttacttct atttaaataa attatcatat tatatatttg gtgaacaagg
     5341 gtattacttt ttatttttca tttgttcttt tctaacatat tatttcatat ttagtattgc
     5401 atatcaaaat aacatattaa caactagcat ttcattatta ttctttagtg ggtttgtttt
     5461 ttttagtaat aatgctataa gacaagcact agtaatttct atatttattt ataatttaaa
     5521 aaacttagaa aaaaaccatt ataaatatct aataataact atattaagtt caactcttat
     5581 acattttagt gcacttttat tcttgatact ttttttattt cctaaaaaga aaaatatacc
     5641 tacaattttt ctatgtatat taattccttt tatttttact ctatatcaat ttgatattgt
     5701 aaacttgata ataaaaaaaa taattatata tataccaaaa tatggtgaac tctattataa
     5761 tagagtcatt gaatttgaat caaaagaaaa agggtctgga ttagttatta ttttttatta
     5821 catgttacta atttatttaa tatataatag gaataaaatt aaaacacaat atagcaatat
     5881 ctttataata ggatatattc tgtttttatt agccccacaa gtggaaatgt gggaaagaat
     5941 aatgattcct ttttattata caaatatatt attcattcca ttaataataa aaaacataaa
     6001 gaaaaagcac tcaccaaata aaatactagg gtatgtattt attattttat tatctttttt
     6061 atttatttac caaacaatag aaaacaaaaa taaaaatggt atttcacctt ttaatcactt
     6121 atttttaaag caagtaggta ttgtattaga gtaatacaaa aaatgaatat gaacaataaa
     6181 agactagtat atactgtaat attagatgac tatgactcat tgattgaaat aaaggaaaaa
     6241 aatgaaaata ttgattatat ttgcattaca gataataaaa acataacatc caacacatgg
     6301 gaaataatat atattgaaaa ggaagataat gatagtacta taataaatag aatgtataaa
     6361 atgcttcccc accgtttctt tcctcattat caaacattat atattgatgg aaatattgca
     6421 ataattaaag atataaataa tctttttgat aaatatttaa ctcaaaatag cattgctgct
     6481 ccaaaacatt caattcgaaa ctgcatttat gaagagagtt tagcttgttt gaaagcaaaa
     6541 aaagaaaaag aatacaatat tcatttgtta ctaatgaaat tacatgagaa aaagtatcca
     6601 tttaataatg gattgttaga gaataatatt ctatttcgta aaaatgaaaa aaaaattaat
     6661 gacgtcatgg aagattggtt tgttattttt aaaaatcttt gtcaaagaga tcaattatct
     6721 ttatgctatt tactatggga aaataacata aaatatcaat tcgtaaaaga aggaccaaga
     6781 tatgagagaa attattttaa aataaacttc cataacaaag aaaaaaaatt aaacatttta
     6841 aaaaaaattc acttatactc aaaactaaac aataaaaaca gtaaactagc ctttattatt
     6901 aataaaataa ttgagacatt actacaaaat agaaaattaa aaaaatgatt aatgctatta
     6961 ttattacata taatcccgac cttaatatat taaagaaaaa tattttaact ttatctacat
     7021 caaaaaaaat aaatgaaatt atcattattg ataattctga taacgctgat aattctattg
     7081 aattttcaaa aataaataac acaactaata gagttaaatt tttcattctt aaagaaaata
     7141 aaggaatagc ttttgctcaa aatattggct taaagtattc agtaaaaagt ggtctagagt
     7201 atgcaatatt atttgatcaa gattcattta tttctagtga tttaataaat ctacttttaa
     7261 taggaattaa agaatgtaat gaattaaata taaagattgc cgcagttgga cctaggccat
     7321 atgatatttt agaaaaaaga aaaagcaaat caattataca aaaagagaac attataaata
     7381 aaaacattac tgtctgtagt caaataattg cttcaggaaa attaataaat ttatcttgct
     7441 taaacactgt tggacttatg gatgaagatt tatttattga tggtgttgac catgagtggt
     7501 gttggagagc caaaagaaaa ggctataatg tagccataat agaaaatgca ataatgcaac
     7561 atacattagg tgatagtcgg ggtaatttct taggcttaac ctataaaata ggggctcctg
     7621 taagattata ttatcagttt agaaatataa ttatattatc acgaagaaac tatgttccat
     7681 cttattggaa aatgcgaaat ttattaggaa tgatatttag atttatcatt ttttcttgct
     7741 ctacaaatga gaaaaaaaca cgaagaaaat atatgataaa aggtttaatt gatggcttaa
     7801 aaaataaaaa gggaagtgtg aaaaactcaa ataaataatt atgaaaaatt atcatattgc
     7861 tgttgccatg tcagtatata aaaatgataa tcctaaattt ctttatgaat caataataag
     7921 tattttatct caagaagaag tcaatgttac cttattcata caaatagatg gtatgattag
     7981 tgacaaatta aaaaaaattt taattctctt taatgaacac tacaatatta atttattttg
     8041 gaataaagaa aataaagggt tagcaactcg tttaaatcaa atcatagatg aggtaataaa
     8101 agaaaataaa tttgattatc ttgcaagaat ggatgctgat gacataagcc atcctaggcg
     8161 ttttatcacc caagctacat ttttagataa acataaagat atctctgtcg ttggaagtga
     8221 tgtaattgaa atatcagaaa acggtgaaca attattttat aaaaaaatgc atagcaatca
     8281 cgatgatata gtgtcaaatg taatacaaaa atgtccattt aatcatccat cagtgatgtt
     8341 taatattgca gtttttaaag aaaattttcg ttataagccg tcattaaaaa acacacaaga
     8401 ttattatctg tggattgatc ttattttcgc aaaaaagcgg ttagctaata taaatttacc
     8461 tttattatgg tttagagtag ataataattt tcatcaaaga aggggaatac agaaatctct
     8521 aaatgagtct ttttcaagaa tctatgccat aaaaaaatta aaaatttatt cagttagaaa
     8581 tattctacat attcctttgc ttttcatgct cagaatatct cctgaagtaa taaaaaaaat
     8641 tgcttataaa aagtatagat aattaattta ttataggata aaaaatgaaa atcaccatct
     8701 ctggtaccgg ctatgtcggt ctatctaacg gaatacttct ttcccaacac aacaaagttg
     8761 ttgcgttaga tatcaataaa gaaaaagttg aattactgaa taaaaaaatc tctccaatta
     8821 cagataaaga aattgaagaa tttttagcaa ccaaagagct cgattttact gcaaccttag
     8881 ataaagagtt cgcttataaa gatgcggatt ttgttatcat tgctacacca actgattatg
     8941 atcctaaaac aaattacttt aatacctcct ctgtagagtc tgttatccgt gatgttttag
     9001 aatataatcc aaacacgaca atgattatta aatcaaccat tcctgttggt tttacgaaag
     9061 aaatgagaga aaaatataat acggataata ttattttctc ccctgagttc ttacgtgaag
     9121 gacgcgcgct ttacgataac ttataccctt ctcgtattgt gattggtgag caatcagaaa
     9181 gggcacaaaa atttgctgac cttttacaac aaggcgcact gaaaaaagat attgatgttc
     9241 tattcactga ctcaacagaa gctgaagcca ttaaattatt tgctaatact tatttagcac
     9301 tccgtgttgc ttactttaat gagctagata gttacgctca ggcttatgac ctaaatgcaa
     9361 aacaaattat tgaaggtgtc tgtttagatc cgcgtattgg taatcattat aataatccat
     9421 cgtttggtta tggtggttac tgcttaccaa aagacaccaa acaactatta gcaaactacg
     9481 actcggtgcc taataatatt attagcgcaa tcgttgaagc taaccgcaca cgtaaagact
     9541 ttattgctga ctcaattatc tctaaatcac caaaaatcgt gggtgtttat cgtttaatta
     9601 tgaaatctgg ctcagataat ttccgttctt cttctatcca aggcattatg aaacgcatta
     9661 aagccaaagg gattgaagtg gtgatttatg aaccagaaat gaaagaagaa accttcttta
     9721 actcaaaatt agttcgtgat ttaaatgagt ttaaagcaat gtctgatgtc attattacca
     9781 atagaaagtc atcagcacta aacgatgttg aagaaaaagt ttatactcgt gacctctttg
     9841 gtaacgatta attaataaat acgggttaat tacgtaatga aatatttagt cactggtgca
     9901 tcagggttca ttggttttca tctaataaaa aaactgatcc aacaaggtga aactgttgtt
     9961 ggtatcgata atctcaatga ttattatgat gtctccttaa aaaaatctag gcttagcatt
    10021 ctgaataaac taaataattt ctcatttgct cttattgacc tagctgatag agaaaaaatg
    10081 gctcgtctat ttgaagctga gaagtttgat aaagttattc atttagcagc tcaagctggc
    10141 gttagatatt cactagttaa cccattttct tacgctgaca gcaatttaac tggctttctg
    10201 acaatcctag aaggttgtcg tcacaatgat attaagcatc ttgtttatgc ttcttccagt
    10261 tctgtttatg gcttaaatga cgaattgcca ttttcacctc atgatcaagc taatcaccct
    10321 gtttctttat atgcggcaac gaaaaaagct aatgagttaa tggctcacag ctattctcat
    10381 ttatatggta taccaacaac tgggttacgc tttttcactg tctatggtcc ttgggggcgc
    10441 cctgatatgg cgttatttaa atttacaaaa gccattatca atgatgagcc tattgatatt
    10501 tataataatg gtgaaatgaa acgcgacttt acttatgttg aagatatcgt ggaaggagtt
    10561 gctcgtattg cagatgtaat acctgcacca caacaagatt ggaaagtaag tataggaacc
    10621 ccagctaaca gttctgcacc ttataaagtt tataatattg gtaatggatc tccagttaat
    10681 ttaatggatt atattagtgc attagaagct catttaggaa aaactgcgga taaaaatatg
    10741 ttaccaatgc aacctggtga tgtttatacc acatgggcag atactgagga tttatttaag
    10801 gcaactggct ataaacctca aacaagcgta gatgaaggtg taaaacagtt tgttgattgg
    10861 tataaaaact attatcaagt taaataaacc atgctcaata tcgtcttatt tgaacccgaa
    10921 attccaccga atacgggtaa tattatccgc ctgtgtgcta acacaggctt tcatcttcat
    10981 ttgatccaac ctttgggttt tacttgggat gataaacgtc ttcgtcgtgc aggacttgat
    11041 tatcgtgaat ttgctgatat taaacagcat cacgattact atgctttttt agaaagtgaa
    11101 ggtttaaacc ctgataatgc acaatcacca acgggtgcgc gtgtatttgc gttaacgaca
    11161 aaaggtacac cagcacatag caatgtaagc tatcaagatg gagattatct cctttttggc
    11221 cctgaaaccc gtggtttgcc accttatgtg ttagataata tgccaacaca gcagaaaatc
    11281 agagttccaa tgcttgctga tagtcgcagt atgaacctgt caaataccgt tgcagttgtg
    11341 gtttttgaag catggcgtca acttggttat cctggtgcgt tattaagaga ctgaaatgca
    11401 aaaagcgctg atcataaata atgagcaagc gcttttttaa tttcaaatga ctaaaaactt
    11461 aatttattat tacattgaga gttgttttat ttcagcaaca accttcgtga gatacctaca
    11521 cccttcccct acatccttca ataccaatgc gtgagcacca atcactgcat tatcaccaat
    11581 ctctatctca ccgccgataa tcgaagaatt acaaccaata taaacgtggt tgccgactcg
    11641 aatatctgct ttaccatcct tttcttttac tccgatggtc acaccttgca ttaacgtcac
    11701 attctcacct aattgacagt aattagtcag cacaataccg ataggatgtg gtaatgaaag
    11761 gccttttcct atgcgcgttc ttaatgagat atcattgcaa tatttacgat tgtttttagc
    11821 accaatgtaa atgcctaatt ttttgagaaa acgattttta gagaaataaa aaaattgagc
    11881 gacacgccac caaaacagat aacgtgaacg ttggcagaaa aagacgcgat gaaaaagtct
    11941 tatccaacta aaaggcttgt tatcaccgat atattcagcg tgtaaacaag ccttaagttc
    12001 agaaaatgta acttctgtta atgccattaa atgccatcac cacattcaaa accaggtact
    12061 acaccattaa agtgttgatc catatcgagt gaaggtttat cactcgtcgg cttaccaaca
    12121 atgcgagcag gaacccccgc aaccgtggta tgtgcaggca ctgcatgtaa tacgacagat
    12181 cccgcaccaa ttttagcacc gcgaccaatc tcaatattgc cgagtatttt agcacccgca
    12241 ccaatcatca caccttcacg cactttagga tgacgatcac cacaggtttt acctgttccg
    12301 cctaaagtca cagattgtaa aatagaaaca tcattctcaa caacagctgt ctcaccaatt
    12361 acaatgcctg ttgcgtgatc aagcataata ccgtggccaa tacgtgcagc tgggtgaata
    12421 tcgacaccaa acgtaacaga aatctggttt tgtaggtaaa cagcaagcgc tttgcgatct
    12481 tgtccccata accaatgtgc tatgcgataa gcctgcaacg catgaaagcc ttttaaataa
    12541 agcaacggtg tggagtattt atcaactgcg gggtcgcgta aacgaacagc tgaaatatca
    12601 taagccgcag attcgatcat ggaatcatcg ctacgatacg cttcttcaac aatctcacga
    12661 acggcaatcg caggcattgt ctgtgtcgcc agcttattcg ctagcatata acttaaggcg
    12721 ctacctaagt tttcatgttt gagtaatgtc gcatggaaga aactcgccaa aatcggctcg
    12781 caatcagcca aacatcttgc ttcattttta atatgatccc agactctttt tagttcttct
    12841 agcgacatac tcactctcat tctttttaca ttgaaaccta agtaacaact taagcttctt
    12901 atgttgaaaa ccgaaacaga caccttaaac caatgcgtta agtagcgatt atttaaaata
    12961 gacttagaac agagtgctgt ctatttcatc ttttgttgcg cgacctaata aagatagtgc
    13021 cgcctctttc gcgtctttat gttggtataa aatttggtag atctgctcag tgataggcat
    13081 ttcaacaccg acttgttctg ctaaggcgcg aacttcttta gtgttccgat aaccttcaac
    13141 cacctgcccg attttctctt gggcagtatc aacatcaagc ccttggccta acatcatacc
    13201 aaatcggcga ttacgggatt ggttatcggt acaagttaaa acgagatcgc ctaacccggc
    13261 cattcccata aacgtcgcag catctgcacc taatgcttta cctaaacggc tcatttcagc
    13321 aagtccgcgg gtaattagtg ctgtacgtgc attcgcgcca aaccccatac catcagacat
    13381 tccagcacca atagcaatca cgttttttac cgcgccacct aattgcacac cgataaagtc
    13441 aggattttta tacacacgga aacttttacc acaatggaac aactgttgaa gctcttcaga
    13501 aaacgtatta tctgttgatg caacagatat cgcagtaggc agacctgctg caagctcttt
    13561 ggcaaaagta ggaccagata aaactgcgag cgggatttca ttacctaata cttcacgcgc
    13621 cacatcttgt aacaagcgac cagtatgagc ctcaagtcct tttgtcgccc aaacaacacg
    13681 tgcatcctga cgtaaaaagg gtttgatctg ctgtaatacg tcaccaaaaa catggcttgg
    13741 gatcacaacc agaatattac ggctagcttc aatggctttt tgcaaagaag cttccatgta
    13801 taaactatca ggaaaagaaa catcgggcag aaatgcctga ttacaacgtg cttgttctaa
    13861 cgccgcaaca tgttcagcat catgacccca aagtacaaca tcatggccat tgcgtgctaa
    13921 ggtaatcgct aaagcggtgc cgtatgaacc ggcaccgata actgtcatag aagcgttcat
    13981 taagcttctt cagccccttc attagcagca tcagtttgtt gctgttgtaa atagttcata
    14041 aacaacgcat caaagttaac tggtgctaag ttcagttgtg ggaaagtacc acggctgact
    14101 aagttagtga tacattcacg agcataaggg aacagaacgt tacggcaata tgcacctaag
    14161 caatgtg
//
