LOCUS       KY710708               15278 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 59/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710708
VERSION     KY710708.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 15278)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15278)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15278
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 59/57"
                     /serotype="O33"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..15278
                     /note="O antigen gene cluster"
     gene            66..587
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             971..2341
                     /note="orf02"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf02"
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="AXY99665.1"
                     /translation="MPRNIAVILASGSGSRFESKIPKQFVKLAGKPVIQYTIEAFENC
                     SAIDEIYIVTKEDYIDLVYEIINKNELKKVSKVISGGKERYDSTWAAINATPTENCNL
                     IIHDAVRPFISERIINDCIAALVTYNAVDVVVDAVDTIVEVDGDFISNIPDRRKLKRG
                     QTPQAFKRDTLKEAYNKFLLDPQKAASDDCGIVLKYLKNEKIYTVVGAESNFKLTHQQ
                     DLYLADNIIKDGLYTRLTTDNNLIKETIENKVIVVIGGSSGIGEDIVTLCEKLNGRVY
                     SCSRSQNNVDVTDKNSIELFLDEVFLKEKKIDFIVNTTGLLIKKPLLSMSDSEITDSY
                     MINYTGVINVALSSYKYLKETQGMLLNFTSSSFTRGRPNYSIYSSTKAAIVNFTQAIA
                     EEWLPLGIKVNCINPERTDTPMRRSNFGIEPPNSLLSAEAVAKFTLSAMSFEHTGQVF
                     SIKNDL"
     CDS             2404..2550
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="wzx"
                     /protein_id="AXY99666.1"
                     /translation="MQYPYLTRVIGAEFLGLYIFSLSIINIANIITSFGFDISISKNI
                     AKKK"
     CDS             3146..3571
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99667.1"
                     /translation="MARTGNFKVFFKITLFSILITLLGSSIGYFFGEYIITKIYSNTL
                     IDAYNVLNVFMLTIIISIIGIHFGYPALIPLKKEKIANYSVLISGILQLLMIFIWWFF
                     NKPFTALTIAYMYFLCDLIMTLIRLYYFGSNYFNFKKNP"
     CDS             3657..4766
                     /note="orf04"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf04"
                     /product="CDP-Glycerol:Poly(glycerophosphate)"
                     /protein_id="AXY99668.1"
                     /translation="MCIGSSHGFRDNAKYYYLYENSRDIKKYWISNSYSDNVLLQKKG
                     IKSHYKWSLKGLYLSLVSKFHIYDYNIDDINFWTSGRAIKFNLWHGVGIKNIEFKIKI
                     GSSKKHFNKLNLKNIIFSPYIYIKPDFFLSTSDMMDKHFCECFRIHSNAIIHNIYPRC
                     QFFLKNKKKLLNHIKRYESSFLQNLILKKSKEFNKLHLYVPTWRDSGKDFISKINWHQ
                     LNDTLAEKNELFLIKPHPNTILKTHLELSNILYIPNKEDIYPLMPFTDTLITDYSSVY
                     YDYLLLRDKKIILFLFDYIEYVKNERDLAFDFNEFTAGVKVYNYDDFFNSLFFNGKKS
                     LSDYKKEEKIIHSFWEYTENKKSINDQLISKINHE"
     CDS             4759..5538
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99669.1"
                     /translation="MNNLPLVSIIIPINKDNPYLLEAINSVLNQTYKNIEVIVIANRC
                     NKLLLDKLSQLDNIKIIITEIAYISFSLNLGIHQAKGKYIARMDSDDISLPSRIDTQV
                     SYLEKNTDIAIVGSNIELIDENSKLLNKRITYPTLDKDIKKALIYKCCLAHPTVIFRK
                     DVIESIGGYLYGPISEDYELWLRASLVHNIKFGNINEVLLNYRIHSSQTTSNKNRVKV
                     FTNDITIKFRYLLQTKKISLFGGILYSTLVFIKNILIKAKK"
     CDS             5543..6658
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99670.1"
                     /translation="MASLSFFFLIFNFPLPMIQSSAVLALLINIPLLIKKRYLPLSKN
                     FAKLILAYILILSLTIIITLFNGHDYTLSLAILKVIIFIILSSIIISIYKDKNLLKLI
                     LNAFSIQAIISLLSLISNEFYNITSIFRNTITQEINMHSDGVRGNIIASDPFFLTGYL
                     VPIVLYLLLEYRNNFSKTKTIFYLFCLITIAFLSARTSIIGVIFTILIFTFKEYKNIL
                     YSFLIICSLILIGVFFQENKLISFVFELFYNLFHSNSATTKSSDVLLRMLNSFTLPNF
                     FGEGKWTNGDGTYFGHTDSGYMRTIYYYGIFSIFFYCIYIYLLTLLKFDYDKKFVIFL
                     FIWSAICQIKGEVFIYPAYANSLLLILLLWKMKNEKA"
     CDS             6645..7754
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99671.1"
                     /translation="MKRLNIIVHSFDKSNIGGVTRVISDIANLLVDNTLHVRIISLGK
                     VNTPAFYIKDNIELLSLNMKNYDTRSYKGVMKIFWFINSFLYLTKIKSKEKYKCEIWY
                     STSPPLVYLLPLLKRKGDILIGCTHTSNTYKKNIPFFYELNSFLLKRYEKIIALTIQD
                     LNSYKKIGVSTKLIPNGIDLSNSLNHFTSMEHSNKNIIFVGRLSPEKDPIEALNIFYK
                     SKLFKQGFKLIYYGNGSLEFELKNKIINLQLTQFVKIIKDEINIDNIYKNAYALILTS
                     KIEGFGMVLIEAISKGVPCVAYDCPNGPRNIIQNGLNGYLIPINNRELFIDSLIKIKN
                     IKKDLNFINSIKKFDLVSIKKSYQSLLLELSENGK"
     CDS             7744..8493
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99672.1"
                     /translation="MENNFVSIIMPAFNSDSVIKESIESVLNQTYTNFELIICDDLST
                     DNTKEIVKKYVEIDQRIKLVNNKFKKGAAGARNSCIQASTSRYICFLDSDDLWAPEKL
                     EKQINFMKKHKIAMTHSDYVMFDTKNNKKTIQCPQIINFSNIIKKCDIGCLTVMLDKK
                     LIPYDISFPYTPKEDYALWVYLMKNGIKSYNFGECSSFYRKQEQSLSSSKFKEIQKQW
                     FVLKHIAQLNFINRVKCISLYSINGFKKHFL"
     CDS             8649..9815
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99673.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIRDLDAFKAMSDVIITNRMATALNDVEEKVYTRDLFGND"
     CDS             complement(10328..11884)
                     /note="orf10"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="hypothetical protein"
                     /protein_id="AXY99674.1"
                     /translation="MKNFFISKYTKLIINIIIYVVPIALLLISSKLMTIGVGGESSVN
                     DVVLLTILIIVLSSSRKAFYFIALPIVLLYALYTPIGITFGKPTYQYIASLFATDLLE
                     SGEFLYQIPLINWFCAIAIIILIFSYYFLCRRFNISYYKNRTLICIMVIVSLIDQSPF
                     SFFRNIITSGQKVKEELVKLNSFDKENRWGNTRLTNNSKYDDYILVIGESARKDYHHA
                     YGYPINNTPFMSASNGVLVDGFTSAGTNTIASLRLMLTKPNLKKWEPDYSLNLIDLVK
                     NSGIKTYWISNQGYFGKFDTPISAIANKSDVKIFMKSGTYNSYNISDFELIPRFDKII
                     FGKSKTKRFIVIHLYGSHPMACDRLFDYKKIIEPKDPKYDYLACYISTINKTDDLLKS
                     LVSSLNKNYQDNNRTYSLIYFSDHGQVHNEVNGKILFNNAFAGKRHYDVPIFKISYDD
                     HERNVIKNFKSGLNFIDGIAHWIGIENDKLNIGYDIFTNKDDQNDYGLSDRIKAINKE
                     DDPAIDIKDK"
     CDS             11979..12482
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXY99675.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(12612..13166)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemX"
                     /product="wemX"
                     /protein_id="AXY99676.1"
                     /translation="MQAQAKVTLAELKECLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGNHVYIGCNSSIIGGEIKIGDNAIIGAH
                     ALVLKDVGEGCRYITKVIADIKQI"
     CDS             complement(13169..13990)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99677.1"
                     /translation="MSLEELTRVWDHIKSEARALADCEPILASFFHATLLKHENLGSA
                     LSYMLANKLATQTMPAIAVREIVEEAYRHDSSMIESAACDLSAVRLRDPAVDKYSTPL
                     LYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIMLDHAT
                     GIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEIGRGA
                     KIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDGI"
     CDS             complement(14099..14215)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="gpsA"
                     /protein_id="AXY99678.1"
                     /translation="MPITEQIYQILYQHKDVKEAALALLGRATKDEIDSSLL"
     CDS             complement(14328..15113)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA_2"
                     /protein_id="AXY99679.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRIVALV"
     gene            complement(15113..15235)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5306 a   2478 c   2296 g   5198 t
ORIGIN
        1 ctcgtacggg ggaagtaaga gctagagcgt atgaaaggaa ccagcggcag atggcatgat
       61 catgacttat tagtgctttc tcgcaccagt ataaaaatga gttattacgt gaaaccgtaa
      121 aagcgaatga gctttggaac gatattttag ataatgcgaa atttgaggct gaacagagca
      181 ataaaaccct aacagtaact gccccaccag gcccatggac gatttattgc aatccttatt
      241 cattagcgag tgcctttgaa aatatcgttc gcaatgcctt gcgttattca cattctcgta
      301 ttgaagtggc ctttaccgag caaaatcagg gtatcactat tattgttgat gatgatggcc
      361 caggagttag cccagaagat cgcgagcata ttttccgacc tttctatcgt acggacgaag
      421 cacgtgatag agaatccggc ggtacagggc tgggattggc aattgttgaa accgcaatta
      481 gtcaacatcg aggccatgta aaagccgatg atagcccatt agggggatta agagtggaga
      541 tttggttgcc aagaggaagt gtggtaaata aatcacaaca cgcttaattc agtaataaaa
      601 ttactacatt aaaatattct gccatctatt gaagaatagg tggcatattc tttttattcc
      661 catctttata gatatttcag aatgatcttg ctaattcagt cgatattccg atatttatat
      721 atacctaatc aatgttagaa tacccatcaa ttacttgacc cgcgggtttg aagagtctaa
      781 tctcagagcg gttttgcgta ctgttaaagt atacttatat aacaaactct tatattacag
      841 atagataaga gattgtcgag aaaatttttg gcgctctggg agccgtaaac ccagtgcggt
      901 agctatgtat aataagattt attttcaccc tataattaat aaataaattg ttattattaa
      961 aggataaata atgcctagaa atattgcagt gatcttagct tcaggaagtg gttctcgatt
     1021 tgaatcaaaa atcccaaaac aatttgtaaa attagcaggg aagccggtca ttcaatatac
     1081 tatagaagct tttgaaaatt gttctgctat agacgaaatt tatatcgtca ccaaagaaga
     1141 ttatatcgac ttagtttatg aaatcattaa taaaaacgaa ttaaagaaag tttctaaagt
     1201 gatttctggt ggaaaagaac gttatgattc tacatgggct gcaattaatg caacaccaac
     1261 agaaaattgc aatctaatca ttcacgatgc tgttagacct tttatttctg agcgcataat
     1321 caatgattgt attgcagctt tagttaccta taacgctgta gatgttgttg tagatgctgt
     1381 tgatacgatt gttgaagttg atggcgattt tatttctaat atccccgata gaagaaaatt
     1441 aaaacgaggg cagacccccc aagcctttaa aagagataca ttaaaagaag catacaacaa
     1501 gtttttgtta gatccacaaa aagccgcatc ggatgactgc ggtattgtat taaaatatct
     1561 caaaaatgaa aaaatatata ctgttgttgg tgctgaatct aactttaagt taactcacca
     1621 acaagattta tacttagcag ataatattat taaagatggt ttatatactc gtttaactac
     1681 cgataataac ttaataaaag aaacaataga aaataaagtt attgttgtta ttggaggtag
     1741 tagcggtata ggagaagata tagttactct gtgtgaaaaa ttaaatggta gagtctactc
     1801 ttgcagtaga agtcaaaata atgttgatgt cactgataaa aatagtattg aattatttct
     1861 tgatgaggtt tttctaaaag aaaagaaaat agattttatt gttaacacaa ctggcctatt
     1921 aataaaaaaa cctcttttat caatgagtga tagtgaaata actgatagtt atatgattaa
     1981 ttacactggc gtaattaatg ttgcactttc gtcttataag tatttaaaag aaacccaagg
     2041 tatgctactt aactttactt ccagttcatt tactcgagga agacctaatt atagcattta
     2101 ctcgtcaaca aaagctgcta ttgttaattt tactcaagct atagcagaag aatggttacc
     2161 tttaggtatt aaagtaaact gtattaatcc tgaaaggaca gatacaccga tgaggcgtag
     2221 taattttggg attgaaccac ctaattcact acttagtgca gaggctgttg caaaattcac
     2281 tctatcagca atgtcatttg aacatacagg gcaagtattt agcataaaga atgatttatg
     2341 agccttaaga aaaatataat taatctattt ggaacccagg tcattggtta tctagttcca
     2401 ttattgcaat atccttactt aacaagagtt attggcgcgg aatttcttgg attatatatt
     2461 ttttccttat ctataattaa tatagcaaat ataattacta gttttggatt tgacatatct
     2521 atatctaaaa atatagcaaa gaaaaaatga atcttttgag agtttatctg aattaaattt
     2581 tagtgtagca atagcaaaaa tatttttatt ttttccatgc gtcatccttt tattaattac
     2641 ctatcttttt acagattatt atactgaaca taaaattctt ttaattttta tctttttatc
     2701 tatatttagt aatgccttta atcttatttg gttattccaa ggtgtagaga aattatatat
     2761 atatagtaga ttatttatct ctacaaaagt aatttccctt atttttattt ttatttttgt
     2821 aaaagaaaaa aatgatttaa atatattatt ccttattaca agttgccaat ttactctttt
     2881 atcattaatt agttatatat atatatattc taaaggatgt agatttcgaa ggtcaccact
     2941 caaaaattct ctcctattaa taaaagaatc atttggatat tttttctctc gactaggaac
     3001 ttcaatatat tctacatgtg gtagcttttt tttaggttta ttttctggtt ctttacatca
     3061 agtagctatt tatggagctg ctgaacaatt atataaagca ggagtttatg ccgtttcagc
     3121 aatttcaacc ccccttattc catacatggc tagaactgga aactttaaag tattctttaa
     3181 aattacatta tttagtattt taattacatt attaggtagc tctattggtt acttttttgg
     3241 tgaatatata ataaccaaaa tatatagtaa tactctaatt gatgcttata acgttctaaa
     3301 tgttttcatg ctaactatta ttatcagtat tattggtatt cattttggct acccagccct
     3361 tataccatta aaaaaagaaa aaatagctaa ttatagtgtt ttaatatctg gtatattaca
     3421 acttttaatg atctttattt ggtggttttt taataaacca tttacagctt taactatagc
     3481 ttatatgtac tttctgtgtg atctaataat gactttgata aggttatatt attttggctc
     3541 aaattacttt aattttaaaa aaaatcctta agaaaggatt taaattttta tttacaatta
     3601 taatttttta tcctctctat gtagtttctt ttttaatccc aagaaataaa aaaattatgt
     3661 gtataggaag ttcacatggt tttagggata atgcaaaata ttattatcta tatgaaaata
     3721 gtagagatat taagaagtat tggatatcaa attcttatag tgataatgtt ttacttcaga
     3781 aaaaaggaat taaatcacat tacaaatgga gtttaaaagg tttatattta tctttagtct
     3841 caaaatttca tatttatgat tataatatag atgatataaa tttttggaca agtggtagag
     3901 ctataaaatt taacttgtgg catggtgtag gaataaaaaa tattgagttt aaaataaaga
     3961 ttggtagtag taaaaagcac tttaataaac taaatttaaa aaatattatt ttttctccat
     4021 atatatatat taaaccagat tttttccttt ctacatcaga tatgatggat aaacattttt
     4081 gcgaatgttt tcgtatacac tcaaatgcta ttatccataa tatttatcca agatgccaat
     4141 tttttttaaa aaataaaaaa aaactgctca accatataaa aagatatgaa tcttcatttt
     4201 tacaaaattt aatactgaaa aaatctaaag aatttaacaa gttacatctt tacgttccta
     4261 cctggcgaga ttcaggtaaa gactttatat ctaaaattaa ttggcatcaa ttaaatgata
     4321 cattagctga aaagaatgaa ttgtttttaa taaaaccaca tcccaatact atattaaaaa
     4381 cacatttaga attatcgaat atactatata ttcctaataa agaggatata tacccattaa
     4441 tgccattcac tgacacacta attacagact attcttccgt gtattatgat tatcttttat
     4501 taagagataa aaaaataata ctttttttat tcgattatat agaatatgta aaaaatgaaa
     4561 gagatcttgc tttcgatttt aatgaattta ctgctggagt taaagtatat aactatgatg
     4621 atttttttaa ctctttattt tttaatggaa aaaaatctct aagtgattat aaaaaagaag
     4681 aaaaaataat acattcattc tgggaatata cagaaaataa aaaaagtata aatgatcaac
     4741 ttatatcgaa gataaaccat gaataattta cctttagtta gtattataat tcctatcaat
     4801 aaagataatc cttatcttct tgaagcaata aatagcgttc tgaatcaaac ttataaaaat
     4861 attgaagtaa tagttattgc taacagatgt aataaattat tattagataa attatctcaa
     4921 cttgataata taaaaataat aataacagaa attgcttaca tttccttttc tctaaattta
     4981 ggaatacatc aagcaaaggg taaatatatc gcaagaatgg attcagatga tatttcttta
     5041 ccatcaagaa tagacaccca agtttcttat ttagaaaaaa atacagatat tgctatagtt
     5101 ggttcaaata tagaattaat tgatgaaaat agtaaattat taaataaaag aataacatat
     5161 ccaacattag ataaagatat aaagaaagct ttaatatata aatgttgctt agctcatcct
     5221 acggtcatat ttagaaaaga tgttattgaa tcaattgggg gatatttata tggtcctatc
     5281 tctgaagact atgagttatg gcttagagcc agtttagtac ataatattaa atttggtaat
     5341 atcaatgagg ttttattaaa ttatagaatt catagttctc aaacaacaag caataagaac
     5401 agagtaaaag tatttactaa tgatataaca attaaattta gatacctatt acaaacaaaa
     5461 aagatatcac tttttggggg tattttatat tcaactttag tttttataaa aaatatatta
     5521 ataaaagcga aaaaataata tcatggcatc attaagcttt ttctttctaa tttttaattt
     5581 ccctttacca atgattcaat catcggccgt tctagcttta ttaataaata ttcctttatt
     5641 aataaaaaaa cgttatctcc cattatctaa aaatttcgct aaattaatat tagcttacat
     5701 tttaatatta tctttaacca ttataattac attgtttaat ggtcatgatt acacattaag
     5761 tttagcaatc ctaaaagtta taatttttat tatattatct tcaatcatta ttagtatata
     5821 taaagataaa aacttactga aactaatatt aaatgcattc tccatacaag caataatttc
     5881 acttttatct ttaatatcaa atgaattcta taatataaca tctattttta gaaatacgat
     5941 tactcaagag ataaacatgc actctgatgg agtaagagga aatattatag ctagtgatcc
     6001 cttttttctg acaggctatt tggtacctat tgttttatat ttactattag agtatagaaa
     6061 taattttagt aaaacaaaaa ctatatttta tttattctgc ttaatcacaa tcgctttttt
     6121 gtctgcaaga acctctatta tcggagtcat ttttacaata cttattttta cttttaaaga
     6181 gtataaaaat atattatatt cctttttaat tatatgttct ttaatcctaa taggtgtttt
     6241 ttttcaagaa aataaattaa tttcattcgt ttttgaattg ttttataact tatttcatag
     6301 taattccgcc acaacaaaat cctcagatgt gctattaagg atgctaaact cctttactct
     6361 tcccaatttt tttggagaag gtaaatggac taatggtgat ggtacatatt ttgggcatac
     6421 tgattcaggg tatatgagaa ctatttatta ttacggcatt tttagcatat ttttttactg
     6481 tatttatatt tacttactaa cccttttaaa atttgattat gataaaaaat ttgttatttt
     6541 tctatttatc tggtctgcta tatgccaaat aaaaggggaa gtatttatct atccagcata
     6601 tgcaaattct ttattattaa tattgttatt atggaagatg aaaaatgaaa aggcttaata
     6661 ttatagttca ttctttcgat aaaagtaata ttggtggtgt tacacgtgtt atatctgaca
     6721 ttgctaattt actagtggat aacactctac atgttagaat tattagctta ggtaaggtaa
     6781 atactcctgc attttatatt aaagataata tagaattatt atcactaaac atgaaaaatt
     6841 atgatacccg ttcttataaa ggagtaatga aaatattttg gtttataaat tcttttcttt
     6901 atttaacaaa aataaagagt aaggaaaaat ataaatgtga aatatggtat tcaacatctc
     6961 cacctttagt atatttactc cctttattaa aaaggaaagg agatatatta ataggatgta
     7021 cacatacatc aaatacatat aaaaaaaaca tccctttctt ttatgaattg aatagttttt
     7081 tactaaaaag atatgaaaaa ataattgctt taactattca agatttaaat tcatataaaa
     7141 aaataggagt tagtacaaag ttaattccta atggaattga tttatctaat agtcttaatc
     7201 attttacatc tatggaacat tctaataaga atattatttt tgttggtcga ttatctccag
     7261 aaaaagatcc gatagaagca cttaatattt tttataaaag taaactattt aaacaaggtt
     7321 ttaaactgat ttattatggt aatggatcat tagaatttga actaaaaaat aaaataatca
     7381 atctacaact tactcaattt gtaaaaataa taaaagacga aataaatata gataatattt
     7441 ataagaatgc atatgcttta attttgactt caaaaattga aggttttgga atggtactta
     7501 ttgaagcaat atccaaagga gttccttgtg ttgcttatga ttgtccgaac ggacctagaa
     7561 atattataca aaatggcctt aatgggtatt tgataccgat aaacaataga gaattattta
     7621 tcgattcact aataaaaata aaaaatataa aaaaagatct aaatttcatt aacagtatta
     7681 aaaaatttga cttagtatca ataaaaaaat cttatcaatc actcttatta gaattatcag
     7741 aaaatggaaa ataatttcgt tagtatcata atgcctgctt ttaactctga ctctgttata
     7801 aaagaatcaa tagaatcagt tttaaatcaa acttatacta atttcgaact aattatttgt
     7861 gatgacttat ctactgataa cactaaagaa attgtaaaaa aatatgtaga aatagatcaa
     7921 aggataaaat tagtaaataa taaatttaaa aaaggtgctg caggtgctag aaatagttgt
     7981 attcaagcat caacctctag atatatttgt tttttagata gtgatgattt atgggctcct
     8041 gaaaaactag aaaagcaaat aaatttcatg aaaaaacata agatagcaat gacacatagt
     8101 gattatgtta tgtttgatac taaaaataat aaaaaaacaa tacaatgtcc tcaaataatt
     8161 aacttttcaa atattataaa aaaatgtgat attggctgtt tgactgtaat gttagataaa
     8221 aaactaattc cttatgatat ttcatttcct tatacaccga aagaagacta tgcattatgg
     8281 gtatacttaa tgaagaatgg tatcaaatct tataactttg gtgaatgtag ctcattttat
     8341 agaaaacaag aacaatcatt atcctcatct aaatttaaag aaatacaaaa acaatggttt
     8401 gttctaaaac acattgccca attaaatttc ataaacagag taaaatgtat ttctttatat
     8461 tcaataaatg gatttaaaaa acattttcta tagaaaagtt ttttacatta gataaaatat
     8521 tcagcaaacc ctaacaatcc ctagtatact ttaatgatat actacacaat aatcatatta
     8581 tatgattgct ctcatctata gctgaatttt gtttttaaat ttactacaaa attagataag
     8641 aaaaaaacat gaaaatcacc atctcaggaa ccggctatgt cggtctatct aatgggatac
     8701 tcctttccca gcacaacaaa gttgttgcat tagatattaa taaagaaaaa gtcgaactat
     8761 taaacaaaaa aatctctcct atctctgata aagaaattga agagttttta gccactaaag
     8821 agctcgactt tacggcaact ttagataaag aattcgctta taaagatgct gaatttgtta
     8881 tcattgccac tcctaccgat tatgatccta aaactaacta ctttaacacc tcttctgtag
     8941 aatctgttat tcgtgatgtt ttagaatata atccaaacac tactatgatt ataaaatcaa
     9001 ccattccagt tggttttact aaacagatga gagaaaaata tcatactgat aatattatct
     9061 tctctcctga gttcttacgt gaaggtcgcg ccctttacga taatctctac ccatctcgta
     9121 ttgtgattgg tgagcaatct gcaagagcag agaaatttgc tgagctttta caacaagggg
     9181 cattaaaaaa agatattgat gttcttttta ctgactctac agaagcagaa gcaatcaaat
     9241 tatttgccaa tacttatctc gcactgcgtg ttgcttattt taatgaactc gatagctacg
     9301 cccaagctta cggtctaaac gcaagacaaa ttattgaagg tgtctgttta gatccgcgta
     9361 ttggtaatca ctataacaac ccctcattcg gttatggcgg ttactgctta ccaaaagata
     9421 ctaaacaatt gttagccaac tacgaatccg tacctaataa tattattggt gccattgttg
     9481 aagctaaccg cactcgtaaa gactttattg ctgattctat tatcgctaaa tcaccgaaga
     9541 ttgttggcgt ttatcgttta attatgaaat caggctctga taatttccgc tcatcttcaa
     9601 tccaaggcat tatgaagcgt attaaagcaa aaggcattga agtagtgatt tatgagccag
     9661 aaatgaaaga agagactttc tttaattcaa aactcattcg tgatttagat gcgtttaaag
     9721 ccatgtctga tgtcattatt accaatagaa tggctacggc gctaaacgat gtggaagaaa
     9781 aagtttatac tcgtgatctc tttggtaacg attgaaaaat taatagatat ttctttacat
     9841 aattagattt ttcatcgagt aggagacggg attactcccg tcgtcctctc actccaccgt
     9901 atgtacgatt ccgtatacgg cggttcatgt taactctgga accgcctata ttgctctaat
     9961 agtgataaca aacctgcttt ccttaacaac attccggctt ttattgcctg atgcatgtgg
    10021 cttgctcccg agttccacca tgcccctcgt tgatttggtc actgatcgcc aagctattta
    10081 ttcagataac cccgcatgca tcaacttctt agcacgcgtg acgggcattt ccattgtcgc
    10141 taaagcaagc aacgtaactt actgtttatc cagctatcta atttctctaa atccctcttt
    10201 tactgcgatg tatctaaagt agggagtgga caaggcgcac tatataaaaa gccatttatt
    10261 attaaataaa tggctataac attttatttc aaatactcaa agttcaatgt tgattgtact
    10321 aaataatcta tttatccttt atatctatcg caggatcatc ttctttatta attgctttta
    10381 ttctatccga taacccataa tcattttggt catctttatt agtaaagata tcatatccta
    10441 tatttaactt atcattctca ataccaatcc aatgagcaat cccatcaata aaatttaaac
    10501 ctgatttgaa atttttaata acatttcttt cgtgatcatc ataagatatt ttgaagatag
    10561 gtacatcata gtgccgtttt ccagcgaacg cattattaaa taatattttt ccatttactt
    10621 cattatgaac ttgaccatga tctgaaaaat aaattaatga atatgttcga ttattatctt
    10681 gatagttttt attcaaagaa cttactaatg actttaacaa atcatctgtt ttatttattg
    10741 ttgaaatata acatgctaaa taatcatatt taggatcttt aggttctatg atttttttat
    10801 aatcaaataa tctatcgcaa gccataggat gcgaaccata taaatgaatt acaataaaac
    10861 gtttagtttt actctttcca aagataatct tatcaaaccg aggaattaat tcaaaatcac
    10921 tgatattata tgaattataa gtcccagatt tcataaaaat ctttacatca ctcttattag
    10981 caattgcaga aattggagta tcaaacttac caaaatatcc ttgattagaa atccaatatg
    11041 ttttaatccc actgtttttt actaaatcaa ttaaattaag gctataatca ggttcccatt
    11101 tttttagatt aggttttgtt agcattaatc gtaacgatgc aatagtattt gtccctgcag
    11161 atgtaaaacc atctactaat acgccatttg acgcactcat aaatggagta ttatttatag
    11221 gataaccata agcatgatga taatcttttc tagcactttc acctataact aaaatataat
    11281 catcatattt tgaattgtta gttaatcttg tattacccca tctattttct ttatcaaaag
    11341 aatttaattt aactaattct tcttttactt tctgaccaga ggtaataata tttctaaaaa
    11401 aagagaatgg agattggtct attaaactaa caattaccat gatacaaatt aaagttctat
    11461 ttttatagta ggaaatatta aaccttctac ataaaaaata gtatgaaaaa attaatataa
    11521 tgatagctat cgcacaaaac caatttatta gtggtatttg gtataaaaat tcaccacttt
    11581 ctaacaagtc agttgcaaat aacgatgcta tatattgata agtaggtttg ccaaatgtaa
    11641 tacctattgg tgtatataaa gcgtataata aaactatcgg taaagcgata aaataaaatg
    11701 cttttcttga cgaacttaat actattatta gaatagttaa tagtactaca tcatttacac
    11761 tactttcacc ccctactcca atcgtcatta gtttcgatga gattagtaac aacgcaattg
    11821 gtactacata aattattata ttaattatta gtttggtata ttttgatata aaaaaatttt
    11881 tcatattatt atgtaattct tagtttatca tattttgaca tgaataatta ttatataata
    11941 ttatatgata attattttct aaatcataga tattttatat gctcaatatc gtcttatttg
    12001 aacccgaaat tccaccgaat acgggtaata ttatccgcct gtgtgcaaat acgggctttc
    12061 atcttcattt gatccaacca ttgggtttta cttgggatga taaacggcta cgccgtgcag
    12121 gacttgatta tcgtgagttt gctgatatca aacagcatca cgattattat gcttttttag
    12181 aaagtgaagg attaagccct gataatgccc aatcgccaac aggggctagg ttattcgcat
    12241 taaccactaa aggcacaccc gcccatagta atgtaagtta tcaagatggg gattatctgc
    12301 tctttggccc tgagacacga ggtttaccac cttatgtgct ggataatatg ccaacacaac
    12361 aaaaaatccg tgtgccaatg ttagcggata gtcgcagtat gaatctttct aatacggttg
    12421 ccgtggttgt ttttgaggca tggcgccaat tgggctaccc tggtgcgtta ttaagagatt
    12481 gatttagtgg ttagtttggt taattaaagt tattttaatc catactcatt acttgatttt
    12541 atctttattt ataaactaaa aaaggtgcta atatttattc agcacctttt ttataaacct
    12601 ttgttcaagc attatatctg cttgatatcc gcaatcactt tcgtgatata ccgacaccct
    12661 tcccctacat cctttaatac taatgcatgg gcgccaataa tcgcattatc accaatctta
    12721 atctcaccac caataatgga cgaattacag ccaatataaa catggtttcc tacttggata
    12781 ttggcttttc cccctttctc ttttacacca atagtcaccc cttgcataat agtgacatat
    12841 tcccccagtt ggcagtaatt agttagtaca atgccaatcg ggtgtggtaa agaaaggcct
    12901 ttaccaatac gagtttttaa cgagatatca ttacagtatt tgcggttatt gctagcaccg
    12961 atccgaatac ctaatttatg taaaaagcga tggggagaga aataaaaata ctgtgctacc
    13021 cgccaccaaa ataggtaacg ggaacgctga cagaagaata cccgatgaaa tagtcttaac
    13081 caactaaagg gttttttatc accaatatat tcagcctgta agcactcttt tagttcagca
    13141 agggtaacct tagcttgggc ttgcatagtt aaataccatc accacactca aagccgggta
    13201 caacaccatt aaagtgctga tccatatcaa atgacggttt ttcactatca ggacgaccga
    13261 caatgcgggc aggcaccccg gccactgtag tatgagccgg gacttcatgt aatactaccg
    13321 agccagcacc aattttggca ccacgaccaa tctcgatatt gccaagaatt ttagcccccg
    13381 cgccaatcat taccccttca cgcactttcg ggtgacgatc gccccctgtt ttacctgttc
    13441 cccctaaggt caccgattgt aaaatggaga catcattttc aaccaccgcc gtttcaccaa
    13501 tcacaatacc tgtcgcatgg tcgagcataa taccgtgtcc aatacgtgcc gccgggtgaa
    13561 tatccacccc aaaggtgact gatatttggt tttgtagata aatggctagc gcttggcgat
    13621 tttgttgcca taaccaatga gcaatacggt aggcttgtag tgcatgaaag ccttttaaat
    13681 agagcagtgg ggtagaatat ttatccaccg cgggatcacg taggcgtacc gcggagagat
    13741 cacaagcggc cgactcaatc attgagctat cgtgacgata agcctcttca acgatttcgc
    13801 gtactgcaat ggctggcata gtctgagttg ccagcttatt ggccagcata tagcttaatg
    13861 cactacctaa attttcgtgc ttgagaagtg tcgcatgaaa gaaactggct aaaattggct
    13921 cacaatctgc caatgctcgc gcttcgcttt taatatgatc ccagactctt gttagttctt
    13981 ctagcgacat actcactttc tttttttaca cgaaagccta agtaaatact taggctttga
    14041 attttcaaac aaatggatag ttaaaacaga taccctaaga atattattta aaataaactt
    14101 acaataggct gctgtctatc tcatctttgg ttgctcgccc taataaagcc aatgcagcct
    14161 cttttacatc tttatgttga tataaaatct ggtagatctg ttcggtgatt ggcatttcta
    14221 cacccacttg ttcggctaat gcgcgaactt ctttggtgtt acgataacct tcactacctg
    14281 cccgattttc tcttgggctg tatcaacatc aaaaccttga cctagcatca taccaaagcg
    14341 acgatacgtg attggttgtc agtacaggtt aaaactaaat cacccaaacc agccataccc
    14401 ataaaagttg ccgcatctgc acctaaggct ttacctaacc ggctcatttc ggccagacca
    14461 cgggtaataa gtgcagtacg cgcattagcg ccaaacccca taccatcaga catacccgca
    14521 ccaatagcaa tcacattttt aacagcgcca cccagttgca caccgataaa atcagggttt
    14581 ttatagactc ggaagctttt accacaatga aatagctgtt gtaactgttc taaaaagaga
    14641 ttgtctgtcg aggccactgc aatcgcggtg ggtaaacccg cggcaagctc tttagcaaag
    14701 gtagggccag ataacactgc gagcgggatt tcattaccta atacttcacg ggcaacatct
    14761 tgtaataagc gaccagtatg tgcttcaaga ccttttgtcg cccaaacaac acgtgcatcc
    14821 tgacgtaaaa agggtttgat ttgttgcagt acttcaccaa acacatggct tgggatcaca
    14881 acgagaatat tacggctcgc ttcaatcgct ttttgcaaag aagcttccat atataaacta
    14941 tcaggaaagg aaacatcggg cagaaaggct tgattacagc gcgcttgttc taatgccgca
    15001 acgtgcttag gatcatgccc ccacagcaca acatcatggc cattacgcgc taaagtaatc
    15061 gctaaagcgg tgccgtatga accggcaccg ataactgtca tagaagcgtt cattaagctt
    15121 cttcaactcc tgagtttgct gcatcctgtt gctgttgttg ctgtaaatag ttcataaaca
    15181 acgcatcata gttaacaggt gctaagttta attgtgggaa agtaccacga cccactaagt
    15241 tggtgataca ttcacgtgca taagggaata gtacgttc
//
