LOCUS       KY710709               12494 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus vulgaris strain CCUG 4669 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710709
VERSION     KY710709.1
KEYWORDS    .
SOURCE      Proteus vulgaris
  ORGANISM  Proteus vulgaris
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12494)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12494
                     /organism="Proteus vulgaris"
                     /mol_type="genomic DNA"
                     /strain="CCUG 4669"
                     /serotype="O34"
                     /culture_collection="CCUG:4669"
                     /db_xref="taxon:585"
                     /collected_by="Cultures Collection, University of
                     Goeteborg, Sweden"
     misc_feature    1..12494
                     /note="O antigen gene cluster"
     gene            4..684
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             1030..2088
                     /note="orf02"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf02"
                     /product="acyltransferase 3"
                     /protein_id="AXY99680.1"
                     /translation="MTTYNYNCNRINGLDLFRFIAALFVILFHYTYRGHQTDNFISVS
                     FPEIDFVTKYGYLGVQFFFMISGFVIMMSSHHGSYKKFLISRISRLYPTFWLCCTLTY
                     IIILLLSDNIAISASLKQYVVNLTMLAGFFKVPSIDGAYWSLYVEIYFYVLIFILLLL
                     KKINKLEIYLICWLTITILMNIFHIPIISKLFMTNYSIYFIAGGLFYYCWIDKINTYR
                     LALIIMCIICSITTSVEQANYLSVKYNDHYSSTIISMILFSFYLSMGYIVIYKNSIIN
                     KINWIKLGTLTYPLYLLHQNIGYILINQISNLNIINKYVIILFIISIMIAISYVIGNM
                     IEPRQIKNTKKLLIKIIK"
     CDS             2090..3340
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99681.1"
                     /translation="MKNILRHSVIKNSISLFIIKFVEIGLPLILLPFLSRNLGVIEFG
                     LYFVIVSILSIGYMITDFGFSISAVYKIVNNKNNKNYIAKYVSSIFVIKTILSIIFIA
                     VIIIYLTNSNYNSINLLSYFLISLIIIFQAYQMPWFFQGIEKMKNITIAVVTTKLTNF
                     LSILLLLHIWNDFNSILISFSINGLICSYIYSYLYKKNGFYFQFPTSSKMIINEAKES
                     FSFFLSKIAITLSSSLNSIIISKFLGLNITALYGSAEKLYNGSINMMSPITNALYPYF
                     ARNKKVSLLVYLSIFFTSIAIILSFTVYLNSEKIIIIIFGYKYIEAATYLNLFLILIP
                     INVLSSLWSYPAFSIIKKTSVANFTVLISSFIYLLILIYLYFMNSISVENIIRSIIIS
                     DLITLLTRLIYFRKLYKKNHDQNQ"
     CDS             3324..4517
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99682.1"
                     /translation="MIKINKLASLFLYLLLPMIGFLLSLLSIRRRNNFLYLNSFYISI
                     FIFIIFIYIPPLGDLYRHYQLFEHIDFNNPKIYKLDFLFYFNLIIFKNLCIPFFILPA
                     LYTSISVYLYLKSFEILYNNYSKNHVKITTLIIYVLIILLNISLFTTASGLRFGLSTA
                     LVIFAYSLIISKNKNLVPLIILLFASFMHFSSYLFIIIYFFSKIKLNKLTFIALSSIA
                     IIMSQIIVFLFEYLVQILNYGHSYVLGKWADSSGKNIAGQIKIILNQLPFFLFLFLFI
                     INKNKFKNINIIYWAVIIALLTKFSFTLFLRFSVVPTFIMLYMLLIAYKEKLIIYIIL
                     LFSIIYFLSDSIYGYRRQILLGNMWEALYKPPILKMINIDKDYDFMLNQINKDGLWIN
                     NPVES"
     CDS             4545..5567
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99683.1"
                     /translation="MKIDFVLPWVDGHDQEWQQKKSFYSEKNDNKSNLSFRFRDFDTL
                     KYVFRSIEKNCSWYNKIYLITEGHIPGWLKLNEKIKIIKHQDLYINKNDLPIFNSSSI
                     EMNLCNIDALSEHFVYLNDDTIIFNKLEVDRFFRNGLPVDFLAHSIIPRKKLYGLIKG
                     NDSWINSINNNIHLINQYYSPKEIEKKYLFEKTYSIKNKLNNFLLKYFLSKFIWFEHW
                     HHPQAYNKTTLKNVFSIYKKQMLLCSKNKFRASDDLTHYLYRYYHLVNNKFYPYKYND
                     GLVKNIDNKDVFYELLTELKTKNIYNFICFNDSDLLSESDFLTIKKELTHFLNNHFQE
                     KSSFEK"
     CDS             5574..6362
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99684.1"
                     /translation="MITIVTPAYNAENTINELYDNINLILDEYEINWIIVNDCSTDST
                     INLLEALAKKNKNISIFNFKYNMGPNYARSYGVEKSKTKYILLLDADDLIFTKNIKLM
                     INFITSNNNFDFYFCPILSIKSRKNFNSNLYRDKEEKLFLIKKPTDFIIYGFPHPSSL
                     IIKKDFFIKNNKKNNLKWGEDILLYLILSKNGIGVRWLYPISCYINEGIGRGSQLNIK
                     YRFKLFESLIKESLIGKKKVNSIIFSIFMIFRFTLSYIYKKIFS"
     CDS             6406..7224
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99685.1"
                     /translation="MNNFSILLSVYNAENPIFLDKALSSLYEYQKIKPAEIVVVIDGP
                     INKGLFEIIDNYKNQLNELIKIINLNKNHGLGYALNEGLKHCSYDWIFRMDTDDFCLP
                     NRFEKQIEYITSNPDISLLGSAIEEFDENMENSFGYRINPLENNDIVNYAKKRNPFNH
                     MTVAFKKEAVMAVGGYQHHLFMEDYNLWIRMISAGYKVANLPDVLVKVRAGNSMVTRR
                     KGIKYIKSEYKLAKLKIKTNLDNSFSAYSIFLLRSIPRMLPTSFLSKIYKTLRK"
     CDS             7238..8185
                     /note="UDP-N-acetylglucosamine 4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gne"
                     /product="gne"
                     /protein_id="AXY99686.1"
                     /translation="MLTIIGGSGFIGTRLSDQLTKENIDFKIVDIAKSEKYPERWIFG
                     DVTKPETLLEPLKGSDVIINLAAQHKDNVHPISLYYEVNVDGAKNVCDVAEQLNIKHI
                     VFTSSVAVYGFVEKETGEDGEFHPFNDYGKSKLNAEHQYEAWHKKDSSNTLVVIRPTV
                     VFGEGNRGNVYNLFRQIASGKFLMVGSGNNQKSMAYVENIAAFLRYSTQIESGHHIFN
                     YIDKPDFTMNELTDIIYTALDKRHSHIKVPYAIGLLGGYCFDVLSKVTGKEFPVSSIR
                     IKKFCARTQFKSNSITNTDFKAPVTLEQGIANTVKNEFL"
     CDS             8421..9587
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99687.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDADFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMREKYNTNNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESYGLNSKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEDTFFNSRLIRDLNEFKAMSDVIITNRMSSVLNDVEEKVYTRDLFGND"
     CDS             9599..10102
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99688.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(10176..10385)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenN"
                     /product="wenN"
                     /protein_id="AXY99689.1"
                     /translation="MVSTEVTLSELKACLHAEYIGCNSSIIGGEIVIGDNAVIGAHAL
                     VLKDVEEGCRYITKVVAEIKRPKID"
     CDS             complement(10385..11218)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99690.1"
                     /translation="MRVSMSLEELKRVWDHIKNEARCLADCEPILASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYRSDNSMIESAAYDISAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWGQDRKALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAIVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(11300..12316)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99691.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPAHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIGASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGFDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     SNLF"
     gene            complement(12316..12492)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     4385 a   1963 c   1858 g   4288 t
ORIGIN
        1 tgagcacagc agcgtttaat ttctgatatt tctcatgagt tacgtacacc attaacacgc
       61 ttacaattag ctagcgcatt gttacgtcgt cgtagtggag aaagtaaaga gctagagcgt
      121 attgaaactg aaactcagcg ccttgatggc atgattaatg acttgctggt tctttctcgt
      181 aatcaacata aaaacgagtt attacgtgaa acagtaaaag ccaatgaatt gtgggatgat
      241 attttagata acgctaaatt tgaggccgaa caaagtaata aaacactaca agtgacaaca
      301 ccgccgggag catggcctat ttactgtaat ccatattctc tggctagtgc atttgaaaat
      361 atcgttcgta atgccttacg ttattccaac tctcgcattg aagttgcctt tactgagcag
      421 aaccaaggta tcaccattat tgttgatgat gatggcccag gcgttagccc agaagataga
      481 gagcatatct tccgaccatt ctatcgtact gatgaagcaa gagatcgtga atctggaggc
      541 acaggcttag gtttagcgat tgttgaaacc gcagttagcc aacatcgagg ccatgttaaa
      601 gctgacgata gtccattggg cgggttaaga gttgagattt ggttaccaag agctggggca
      661 agtaataaat caacaactgc ttaatagagt aattaaactg ttgtatcaaa atatactgcc
      721 atctattgaa gaataggtgg catattcttt tagttagcgt ttgttgatgc aattcagaat
      781 gatctggctg aatcgacctc tattccgata tttatatata ctcaatcaat gctaaaatag
      841 ccagctatta cttgaccgtc tggtttgaag agtctactct cactgcggtt tttgcatatt
      901 gttaaagtat gcttatataa caaaactctt atactacata tagataagag tttttcgaga
      961 taaattttgg cgctctgggg gccgtaaacc cagtgcggta gctattatta atgcttaaga
     1021 taccttttaa tgactacata taattataac tgtaacagaa ttaatggatt agatcttttt
     1081 agatttattg ctgcactatt tgttatatta tttcattata catatagggg acatcaaaca
     1141 gataatttca tatctgtttc atttccagag atagattttg tcacaaaata tggttattta
     1201 ggggtacagt tcttttttat gattagtggg tttgtcataa tgatgagctc acaccatgga
     1261 tcttataaaa aatttttaat ttctaggatt agtcgtctat atccgacttt ttggttatgc
     1321 tgtactttaa catatataat aatattactt ttaagtgata atattgctat aagtgctagc
     1381 ttaaaacaat atgttgtaaa tttgacaatg ttagctggct tttttaaagt tccttctatt
     1441 gatggtgcat actggagctt atacgttgaa atttattttt atgtacttat atttattcta
     1501 ttgcttttaa aaaaaataaa caaattagaa atctatttaa tttgctggtt aacgataacc
     1561 atattaatga atatattcca tatacctatc atctctaaat tatttatgac taactattca
     1621 atatatttta ttgcaggtgg tctattttat tattgctgga tagataaaat taatacatat
     1681 cgattggctt taataataat gtgtattatt tgctccatta caacttcagt agaacaagca
     1741 aattacttaa gtgtaaagta caatgatcat tattcaagca caatcattag tatgatatta
     1801 ttttcctttt acttaagtat gggatatata gttatatata aaaactctat aataaataaa
     1861 ataaactgga tcaaacttgg tactttgaca tatccattat atttattaca tcaaaatatt
     1921 ggctacatat taattaacca aatatcaaac ttaaacatca taaataaata tgttattatt
     1981 ctttttatca tatctataat gatagccatt tcttatgtaa taggaaatat gatagaacca
     2041 agacagataa aaaatacaaa aaaactcctt ataaaaatta taaaataaga tgaaaaatat
     2101 acttagacac tcagtaataa aaaacagtat ttctctattt attattaaat ttgtcgaaat
     2161 aggattacct ttgatcctgt taccattttt atctagaaat cttggtgtta tagagtttgg
     2221 attatatttc gttattgttt ctatactatc aataggatat atgataacag attttggatt
     2281 tagtatctca gctgtatata aaatagtaaa taataaaaat aataaaaatt acattgctaa
     2341 atatgtttca tcaatattcg ttataaaaac aatcttatct attattttca ttgctgtaat
     2401 tataatttat cttactaata gtaattataa ttcaataaat ttattatcat attttttaat
     2461 atcattaatt attatttttc aagcttatca aatgccttgg ttttttcaag gtatagaaaa
     2521 aatgaaaaat ataacaatcg cagtagttac tacaaagtta accaattttt tatctattct
     2581 actactcttg catatctgga atgactttaa ttccatttta atttcctttt caattaatgg
     2641 attaatatgt tcttatatat attcctattt atataaaaaa aatgggtttt attttcaatt
     2701 tccaacatca tcaaaaatga ttataaatga agccaaagaa agcttttctt ttttcttatc
     2761 taaaattgct ataacattat caagttcatt aaactcaatt ataattagta aatttttagg
     2821 gttaaatata acagcattat atggttctgc agagaaatta tataatggaa gtattaatat
     2881 gatgagccct ataactaatg ctctttatcc ttattttgct agaaataaaa aagtttctct
     2941 tttagtttat ttatctattt tttttacaag tatagcaatt atactatcat ttactgtata
     3001 tctaaattct gaaaaaataa taatcattat ctttggatat aaatatatag aagcagctac
     3061 ttatctaaat ttatttctta tattaatacc tattaatgta ttatcttctt tatggagcta
     3121 tcctgcattt tctataataa aaaaaactag tgtagccaat tttactgtac ttattagttc
     3181 atttatatac ctattgattt taatatactt atattttatg aattcgatat cagtagagaa
     3241 cattataagg tcaataataa tctccgactt aattacatta ttaacaagat taatttactt
     3301 tagaaaactt tataaaaaaa atcatgatca aaatcaataa attagcatct ctgtttttat
     3361 atttactact tcctatgata ggctttttac tatcactgct atcaataaga agaagaaata
     3421 attttttata tttgaatagt ttctatataa gtatttttat atttatcata tttatttaca
     3481 ttccaccact tggtgattta tatagacatt atcaattgtt tgaacacata gactttaata
     3541 atcctaaaat ttataaacta gattttttat tctattttaa tttgataata tttaaaaatt
     3601 tatgtattcc attttttatt ttacccgctt tatatactag tatatctgta tatctctacc
     3661 taaaatcatt tgaaatatta tataataatt atagcaaaaa tcatgtaaaa ataactacat
     3721 tgataattta tgttttaatt atattactga atattagcct ttttaccacg gcatcaggat
     3781 taagatttgg attatctaca gctttagtaa tttttgctta cagcttaata atatcaaaaa
     3841 ataaaaattt agttcctctt attatacttt tatttgcaag ctttatgcat tttagctctt
     3901 atttattcat tataatttat tttttttcta aaataaaatt aaacaaactc acttttattg
     3961 cactttcaag tatcgcaata ataatgtcac aaataatagt ttttttattt gaatatttag
     4021 tccagatact taactatggg cattcatatg ttttgggtaa gtgggcagat agttctggaa
     4081 aaaatatagc aggccaaata aaaataattt tgaatcagct tcccttcttc ctttttttat
     4141 ttttatttat aataaataaa aataaattta aaaatattaa tattatatat tgggctgtca
     4201 taatagcttt gctaacaaag ttttccttta ctctattctt acgattctct gttgtcccta
     4261 ctttcattat gctttatatg ttattaatag catataaaga aaaactaata atatatataa
     4321 ttctattatt ttctattatt tattttctta gtgattctat atatggatac agaaggcaaa
     4381 ttttactagg aaatatgtgg gaagcattat acaaacctcc catattaaaa atgattaata
     4441 tagataaaga ctatgacttt atgcttaatc agataaataa agatggtttg tggataaaca
     4501 atcctgtaga aagttaaaat aaacgcatca taggtaactt attaatgaaa atagattttg
     4561 ttttaccatg ggtagatggg catgatcaag aatggcaaca gaaaaaatca ttttactctg
     4621 aaaagaatga caataaaagt aacctaagct ttagatttcg agattttgat actttaaaat
     4681 atgtttttag atctatagaa aaaaactgct cttggtataa taagatttat ctaattacag
     4741 agggtcatat tcccggttgg ctaaaattaa atgaaaaaat aaaaataatt aaacatcaag
     4801 atttatatat taataaaaac gatttaccaa tatttaactc ttcatcaata gaaatgaacc
     4861 tttgtaatat tgatgctctt tcagagcatt ttgtatattt aaatgatgat acaataatat
     4921 tcaataaatt agaagtagac aggtttttta gaaatggatt acctgttgat tttctagctc
     4981 atagtataat acctagaaaa aaattatatg gtttaattaa agggaacgat tcttggataa
     5041 actcaataaa taataatata catttaatta atcaatatta ctcccctaaa gaaatagaaa
     5101 aaaaatatct atttgaaaaa acttactcaa taaaaaataa actaaataat tttttattaa
     5161 aatatttttt gtctaaattt atttggtttg aacattggca tcatcctcag gcatataata
     5221 aaacaacatt aaaaaatgtt ttctctatct ataaaaagca gatgttatta tgctcaaaaa
     5281 ataaatttag agcatcagat gatttaacac attaccttta tagatactat catttagtta
     5341 ataataagtt ttatccttat aagtacaatg atggtttagt taaaaacatt gataataaag
     5401 atgtatttta tgaactatta actgaattaa aaacaaagaa tatatataat ttcatttgtt
     5461 ttaatgattc tgatttatta tcagaatccg attttctaac aataaaaaaa gaattaacac
     5521 atttccttaa taatcatttt caggaaaaat catcttttga aaagtgacac tatatgatta
     5581 ctattgtaac tcctgcatat aacgctgaaa acaccattaa tgaactatat gataatataa
     5641 atttaatctt agatgaatat gaaatcaact ggattatcgt aaatgattgt agtactgatt
     5701 caacaataaa cttattagaa gctttagcaa aaaaaaataa aaacatatct atttttaatt
     5761 ttaaatataa tatgggacct aattatgcta gaagttatgg tgtggaaaaa tctaaaacca
     5821 agtatatatt actacttgat gcagatgacc tcatcttcac taaaaatatc aaattaatga
     5881 taaattttat tacttctaat aataattttg atttttactt ctgcccaatt ctatcaataa
     5941 agtcaagaaa aaactttaat tctaacttat atagagataa ggaagagaag ttattcttaa
     6001 taaaaaaacc aactgacttt ataatttatg gattccctca ccctagtagc ttaattataa
     6061 aaaaagattt ttttattaaa aataataaaa aaaataattt aaaatggggt gaggacattt
     6121 tattatattt aatattatct aaaaatggta taggcgttag atggttatac ccaataagtt
     6181 gttatataaa tgaaggtata ggaagaggca gccaattaaa tataaagtat cgttttaagt
     6241 tatttgaatc attaattaaa gaatctttaa tcggtaaaaa gaaagttaac tctatcatat
     6301 tttctatttt tatgatcttt agattcacat tatcttatat atacaaaaaa atatttagtt
     6361 aacctccttt agaaattaaa tgaatatttt tattttggtg tgaaaatgaa taatttttct
     6421 attttactat ctgtatacaa tgctgaaaat ccaatttttc tagacaaagc attatcaagc
     6481 ttatatgaat accaaaagat aaaaccagct gaaatagtcg tagtgattga tggtccgata
     6541 aataaaggtt tgtttgaaat aattgataat tataaaaatc aactcaatga gttaataaaa
     6601 attataaact taaataaaaa tcatggcctc ggatatgcat taaatgaagg cttaaaacat
     6661 tgttcgtatg attggatttt tagaatggat actgatgatt tttgtttacc taatcgattc
     6721 gaaaaacaaa ttgaatatat tactagtaat cctgatattt cattacttgg ttctgcaata
     6781 gaagaatttg atgaaaatat ggaaaattca tttggttatc gtattaaccc cctagaaaat
     6841 aacgatatag ttaattatgc aaaaaaaaga aatcccttta atcacatgac tgttgctttt
     6901 aaaaaagaag cagttatggc tgtaggaggc tatcaacatc atttatttat ggaagattat
     6961 aatctatgga ttcgaatgat ttctgctgga tataaggttg caaatttacc agatgttctt
     7021 gtcaaagtta gagctggtaa ttctatggtt acaagaagaa aaggtattaa atatataaaa
     7081 agtgaatata aattagctaa gttaaaaata aaaacaaatt tagataattc tttttccgca
     7141 tattctatct ttttgctaag aagtattcca agaatgctac ctacttcttt tttatctaaa
     7201 atatataaaa cgttaagaaa ataaataagg tatttctatg ctaacaatca tcggtggctc
     7261 aggttttatt ggcacacgcc tttcagatca attaactaaa gaaaatattg attttaaaat
     7321 tgtagatatt gctaaaagtg aaaaatatcc tgaaagatgg atatttggtg atgtaacaaa
     7381 accagagaca ttacttgaac cattaaaggg ttctgatgtt attatcaact tagcggcaca
     7441 acataaagat aatgttcatc ctattagttt atattatgaa gttaatgtag atggagctaa
     7501 aaatgtttgt gatgttgctg aacagctaaa tattaaacat atcgtattta cttcatctgt
     7561 tgctgtatat ggatttgttg aaaaagaaac tggtgaagat ggcgaatttc atccattcaa
     7621 tgattacggc aaatctaaac ttaatgctga acatcaatat gaagcatggc ataaaaaaga
     7681 ttcttcaaat actttagtcg ttatcagacc aacggttgtc tttggtgaag gtaatagagg
     7741 aaatgtttat aacctatttc gccaaatagc atctggtaag tttttaatgg taggctctgg
     7801 taacaatcaa aaatcaatgg cttatgttga aaatatcgca gcctttttaa gatattctac
     7861 tcaaatagaa tctggtcatc atatatttaa ttatattgat aaacctgatt ttactatgaa
     7921 tgaattgaca gatatcattt atacagccct agataaacga catagccaca ttaaagttcc
     7981 ttatgctatt ggattacttg gtggatattg ttttgatgtt ctttctaaag ttacaggtaa
     8041 agaattccct gtaagcagta ttcgaatcaa aaaattctgt gctcgcactc aatttaaatc
     8101 aaatagtatt actaatactg attttaaagc tccagttact cttgagcaag gtattgctaa
     8161 cacagttaaa aatgaatttt tataatagat tagttttaaa tagtcttttt gttaacaaat
     8221 aattaaaata taccctacac ttctcaactt gctttctaat aaaaaattaa ttttatatta
     8281 atttaataag tatatgatgt ttatgattac taaatacact atacctttac taaagagtga
     8341 aaaaacaaca ctaaaatatg tatttctatt ttagtatagt gaattaatat aaatgcatat
     8401 actttagata agagaaactc atgaaaatca ccatctctgg aaccggctat gtcggcctat
     8461 ctaacgggat acttctttcc caaaacaaca aagttgttgc gttagatatc aataaagaaa
     8521 aagttgaatt gcttaacaaa aaaatctctc cgatttctga taaagaaatt gaagagtttt
     8581 tagcaaccaa agaactcgac tttacggcaa ctttagataa agagttcgct tataaagatg
     8641 cggattttgt tatcattgca acaccaaccg attatgatcc taaaactaac tactttaaca
     8701 cctcatctgt agagtctgtt attcgtgatg ttttagaata taatccgaac acgacaatga
     8761 ttattaaatc aacaattcct gttggtttta ccaaagaaat gagagaaaaa tataatacga
     8821 ataatattat tttctcccca gaattcttac gtgaaggtcg tgcactttac gataacttat
     8881 acccttctcg tattgtgatt ggcgagcaat cagaaagagc acaaaaattt gcagaccttt
     8941 tacaacaagg tgcattaaag aaagatattg atgttctttt cactgattca acagaagcag
     9001 aagccattaa attatttgct aatacttatc tagcactgcg tgttgcttac tttaatgagc
     9061 ttgatagcta tgcggaatct tacggtttaa actcaaaaca aatcattgaa ggtgtctgtt
     9121 tagatccgcg tattggcaat cactacaata acccatcatt cggttacggt ggttactgct
     9181 taccaaaaga tactaaacaa ctattagcaa actacgactc tgtccctaat aatattatta
     9241 gtgcaatcgt tgaagctaat cgcactcgta aagactttat tgcagattct attattgcta
     9301 aatcaccaaa aatcgtgggt gtttatcgtt taattatgaa atcgggttct gataatttcc
     9361 gctcatcatc aatccaaggc attatgaaac gaattaaagc caaaggcatt gaagtggtga
     9421 tttatgaacc agaaatgaaa gaagatactt tctttaattc aagattgatc cgtgatttaa
     9481 atgagtttaa agctatgtct gatgtcatta ttactaatag aatgtcatca gtgctaaacg
     9541 atgttgaaga aaaagtttat actcgtgacc tctttggtaa cgattaaatc gatttcatat
     9601 gctcaatatc gtcttatttg aacccgaaat tccaccgaat acgggtaata ttattcgcct
     9661 gtgtgctaac acaggctttc atctccattt gattcaacct ttgggtttta cttgggatga
     9721 taaacgtcta cgtcgtgcag ggcttgatta tcgtgaattt gcagatatta aacagcatca
     9781 cgattattat gcttttttag aaagtgaagg tttaaaccct gataatgcac aatcaccgac
     9841 aggtgcgcgt gtatttgctt taacgacaaa agggactcct gcacacagta atgtgagcta
     9901 tcaagatgga gattatctcc tgtttggacc tgaaactcgt ggcttgccac cttatgtatt
     9961 ggataatatg ccaacacagc agaaaatcag agtccctatg cttgcagata gtcgcagtat
    10021 gaacctatca aatactgttg ccgttgtcgt tttcgaggca tggcgtcaac ttggttatcc
    10081 tggtgcatta ttaagagact gaaatgtaaa aagcgctgat cattaataat gagcaagcgc
    10141 tttttctatt gaatccaaag atattaaaac ttcgtttagt ctatttttgg ccgttttatt
    10201 tcagcaacaa ccttcgtaat atatcgacac ccttcctcta catctttcaa caccaatgca
    10261 tgagcgccaa tcacggcatt atcacctatc actatctctc cgccaataat cgatgagtta
    10321 cagccaatat attcagcgtg taaacaagcc ttaagttcag agagtgtgac ttctgttgat
    10381 accattaaat accatcacca cattcaaaac cgggtactac accattaaag tgttgatcca
    10441 tatcgagtga aggcttatca ctggttggtt tacccacaat gcgagcaggt acgccagcaa
    10501 ccgtggtgtg tgcaggaact tcatgtaata ccacagagcc agcgccaatt ttagcaccgc
    10561 gaccaatctc gatattgccg agtattttag cacctgcacc aatcatcaca ccttcgcgta
    10621 ctttaggatg gcgatcgcca caggttttac ctgtaccacc taaggtaaca gattgtaaaa
    10681 tagagacatc attctctacg atagccgttt caccaatcac aatacctgtt gcgtgatcga
    10741 gcattatgcc atgaccaata cgggcagcag gatgaatatc aacaccaaaa gtaactgaaa
    10801 tctggttttg taggtaaatg gcgagtgctt tacgatcttg cccccataac caatgggcta
    10861 tacggtaagc ttgtaaggca tgaaaacctt ttagataaag cagaggtgtg gagtatttat
    10921 caacagcagg atcgcgtaaa cgaacagcag aaatatcata agctgcagat tcaatcatag
    10981 aattatcgct gcgatatgct tcttcaacaa tctcacgcac agcaatcgca ggcattgtct
    11041 gagttgccag cttgttcgcc agcatataac ttaaagcgct gcctaaattt tcatgcttga
    11101 gtaatgtcgc atggaagaaa ctcgccaaaa ttggctcgca atctgccaaa catcttgctt
    11161 catttttaat atgatcccag actcttttta gctcttctag cgacatactc actctcattc
    11221 ttttacattg aagcctaagt aaaaacttag gcttcttacg tttaaaacag aaataaataa
    11281 ctgactatct aaaatagact tagaacagat tgctgtctat ctcatctttc gttgcgcggc
    11341 ctaataaaga taatgccgct tctttggcat ctttatgctg atataaaatc tggtagatct
    11401 gctcagtgat aggcatttca acaccaactt gttctgctaa tgcgcgaact tctttggtat
    11461 tacgataacc ttcaactacc tgcccgattt tctcttgggc agtatcaaca tcaaaacctt
    11521 gacctagcat catgccgaat cgacgattac gagattggtt atcagtacag gttaagacta
    11581 aatcacctaa ccctgccatt cccataaagg ttgctgcatc tgcacctaat gctttcccta
    11641 aacggctcat ttcagcaaga cctcgggtaa tcagtgcggt acgtgcatta gcaccaaagc
    11701 ccataccatc agacataccc gcaccaatag caatcacgtt ttttaccgcg ccacctaatt
    11761 gcacaccgat aaagtcaggg tttttataaa cacggaagct tttgccacaa tggaacaact
    11821 gttgaagttc ttcagagaag gtattatccg ttgatgccac agaaatcgca gtaggtagac
    11881 ctgccgcaag ctctttcgca aaggtagggc ctgataaaac tgctagcggg atttcattac
    11941 ctaatacttc acgcgctaca tcttgcaata agcgaccagt atgtgcctca agtccttttg
    12001 tcgcccaaac aacacgcgca tcctgacgta aaaagggttt gatctgttgt aatacatcac
    12061 caaaaacatg gcttgggatc acaaccagaa tattacggct ggctccaatg gctttttgca
    12121 aagaagcttc catgtataaa ctatcaggaa aggaaacatc gggcagaaat gcctgattac
    12181 agcgagcttg ctctagcgca gcaacatgcg caggatcatg accccaaagc acaacgtcat
    12241 gaccattgcg tgccagtgta atcgctaaag cggtgccgta tgaaccggca ccgataactg
    12301 tcatagaagc gttcattaag cttcttcagc cccttcatta gcggcatcag tttgttgctg
    12361 ttgtaaatag ttcataaaca acgcatcaaa gttaactggt gctaaattca gttgtgggaa
    12421 agtaccacgg ctgactaagt tagtgataca ttcacgagca taagggaaca gaacgttaag
    12481 gcaatatgca ccgg
//
