LOCUS       KY710710               16307 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 62/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710710
VERSION     KY710710.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 16307)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16307)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16307
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 62/57"
                     /serotype="O36"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..16307
                     /note="O antigen gene cluster"
     gene            3..641
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             952..2052
                     /note="DegT/DnrJ/EryC1/StrS aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="vioA"
                     /product="vioA"
                     /protein_id="AXY99692.1"
                     /translation="METITVTSPLFPPLEELEPYLQDIWKRKWLTNNGYYHQRLEEAL
                     CKYLNVPYISLYSNGTLALIAAINVLKLKGEVITTPYSFVATSHSLLHSHITPVFVDI
                     EPNTYNIDPDKIEAAITDKTTAILPVHVYGRPCNHEAIQKIADKYNLPVIYDAAHAFG
                     VKENGNSILNQGNLSILSFHATKTYSTIEGGAIICHSAEMKEKLDKYKNFGYTNETEI
                     DELGFNAKLNEVQAAFGLLTLNYIDKAIEKRKEVSDFYKTNLSNVPGVTLPNIPKDLT
                     WNYSYYPIIIDKDRYGKTRDELYLELKENKILSRRYFYPLITDFNIYNQYKTKTPTAE
                     KISENVICLPIHHLLSNDDLNRIINVIKYDYK"
     CDS             2039..3091
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99693.1"
                     /translation="MTINKLINPLPEKEIIKKWDSEEVFVSVICCTYNQEKYIEDTIL
                     GVLCQDTPYRYEFIIHDDCSTDSTSEIIKKYADNYPKIIKHIIPAKNIYSQGIQPGIN
                     AFSFSNGKYIALCEGDDFWIDEKKLTKQVDILENKKDINICFSSAYTLQPNGKIDLTC
                     NYHNQIFCAPLSTVIRQGGAYMPTASLLFRRDVFIQFKNNFSKASIGDYYCQILGSIN
                     KGAIYLPDITCVYRINSLGSWSKSRINVSKEILISEIDEYERNLYKLIILGADKKDID
                     YSLADICRVNANELFLRKDWSGCKSLIIKSWIFNKKINRNQEKLYLYRNILPILYLRE
                     KIKKSLKKISLKRIKL"
     CDS             3091..3783
                     /note="WbqC-like family protein"
                     /codon_start=1
                     /transl_table=11
                     /gene="wbqC"
                     /product="wbqC"
                     /protein_id="AXY99694.1"
                     /translation="MRLAVMQPYLFPYIGYYQLAYYADKFIFYDDVNYIKGGYINRNN
                     ILTKNGLQMFTLPVNKASSFKKINELYFQDNTKKILTSIYQNYSKTAYFHSIFPIIEN
                     VLTNNNRNVANITSLSIIKIFEYLSLKFNYTFSSDQNYNRTYNAQEKIYELCKINDAQ
                     EYINPIGGKDLYSKNEFKKRNIKLNFLEPILSPYTQINSSNFVSNTSVIDILMNCNKD
                     EIINQFNNFKII"
     CDS             3792..4358
                     /note="galactoside O-acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="vioB"
                     /product="vioB"
                     /protein_id="AXY99695.1"
                     /translation="MSYYTTEQLAEIGFKKIGTNCKISTKASIYRPEEIEIGNDCRID
                     DFVILSGKIHLKNNIHIAAFCNLSAGECSILFEDFSGLAYGCHVFTGSDDYSGRTMTN
                     PTIPLKYKNLKEKNIIIGKHAIIGTNSIIMPGVTIAEGTAVGALSMVTKSTKPWGIYF
                     GNPAKRIKERKKDLLNLEMEYIKEKSKK"
     CDS             4909..6111
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99696.1"
                     /translation="MKIVSFTIVWYLIFFLIAISGLTGLQTPSQIAITSIGLILSSYL
                     IGCITSFLLPRTKKNVYETKNYNKKRKNNFEYIIKIFLFYFISHSLFIIINVFLINKI
                     DINNYRESFFDGSRKNLIFFGGWWLFYLYYLSTLFLLALVPFFVATKKINIQILLFIS
                     VILYDIVFLSRTGIYYFLLATICAVIIRELKLKLIFLLVVVIIMLSLTISYFRGDISN
                     IFILIKNAIINYHVAPYILFDKNIISENTIRYNGLGLASLGIYNIFLYIFDNNVMENI
                     NNLRANLNVFYDLSSNDNYIPYNAYYTSLGLIYIDFGLIGCIIISYFSGLFISYLAIK
                     SKNSEKYKISCVFISSLYLESLFSPITINIFSFVIVVFILLLMIHNYEYSNCSPVFKK
                     NCSSKYSN"
     CDS             6050..7114
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99697.1"
                     /translation="MNIAIVVPSLRKTAPVNIAISIYKDIKSYCNVHATLFYLDKIKD
                     IDNNINNIPEAILLNRKNYKLLKNFDIVHTHGLRPDIISFLYAKKKSRVTTLHSMIIK
                     DLISRYGIKGFFISYMWIFILIFFNKRVSISNSVKKHLIKKKISSTVIYNGIKFDSFN
                     IKNDKEYLKIDCYINSIKKNKIVVGTISHIEKIKGIEQLIELAKLNENIFLIIIGDGT
                     YKYKIEKLVSLYNLENRILFTGHINHNAQYYSKLFDVYVQPSINEGFGLSVIEAICNK
                     IPVVCSNIEVFTELFNKQQISFFQLNDINSLNNAIQKATTKNTTELETIANTIKDRFS
                     VSTMSKNYINLYETIIKNER"
     CDS             7104..8282
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99698.1"
                     /translation="MKDKLIIFLCYGVNFIIPILFFRFISERLDDINLATFFFHLAII
                     FYFQYVIEYGFQVSLIKFLFKIKNNKKKINIIISSVIYFRLFLFLLAFLIVSLFFYIL
                     VYDKSFLYIYLILIGNIFSFQFLYQIFNELKIYYILSLFSKLLFLPLIFISDNITTLY
                     ILFNIIPNLILFAFIIKKYKIFFLILNIQIIFKIAKNKFSYFASNLSITLYTNFNQII
                     LGLFHPLTLPAYALADKIIRSIVAGNYIFIQICQIKFLDNANLKNKKLRNKFITLFFI
                     LGIVELLFLVSLSYPINKYLFPNILNINTFIIILSLLLPIILMSNLYGMVFLTCSNKT
                     KLLSRVFFSAASIAIMTSPFLIYLYDGFGALIASIIAELIVLILCMKYYSTIFGNRNA
                     "
     CDS             8263..9081
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99699.1"
                     /translation="MEIEMPNVAIIMSIYANDILSQVKEAIDSIFSQNYNNIRIFICI
                     DGEINQDIMNYLNSLKKIFLIKRDKNKGLAISLNELIECVLKEDRFKYIARMDADDIS
                     MPDRIQKQVQYLEKNLDISVLGTKCKEFGSIHAREYCSVYESNNDIYKYIFKKCPFVH
                     PSVMFRTEVFKSGIRYPTNEPFTEDLALWFILAMKKYKFSNLNECLLKFRINDQTVSR
                     RRGYKKAIIEFKIRLKYLILLKRINNIHDIYYTLGHFFVRLLPTPLFKRIYIKG"
     CDS             9085..10884
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXY99700.1"
                     /translation="MKKILYSYDIWDTILKRHCPPKDTIRISVYIFLLYYGITQHLND
                     ILENIFQKENEYFNKNKEYYIFDLLYQEVKKIKNLDLTNIDKKQLKKIWDNIYINVEK
                     KSTYPNKKILELKDKDKGEKIYISDFYIEKKLLDNILNHHNIYFNDGFVSCDENKNKY
                     SGDLYEKIKNIYHTNSINWVHTGDNKISDIKVARSLKIKTRYVNKFNLSNKIKKLFPV
                     KKQYNDIENLSIILIGFSKFILEECKKNKVNDIYFFTREGVTFKRFFDLFLKEISSNF
                     NINFNIKTHIVYVSRTATIPLRLDINDSYLGFKDAILQYGNDTNTFLSFFNLSKKLAH
                     LSEKYKKIEELLKNEKDKRILIELISNKKNLAINYLESLGFFEKNKLIVDIGWRGSIQ
                     DNLSHIDKKIKLGCYLGLFEFFQNQNNAKKEGYIFNNNTNKESIFHKGVDFWETIFNT
                     KGGSVTSYVNNIPKKKINKDEELFLDEVINVQEIIYNEAKHRLHDIIKNKLSLLDIDN
                     HARLAYKKISTRPSSYLADLYLSSLQNENYGLNSFKRKYINITIKSLIKSFFNKKEKE
                     SISNLIKNNGWRESILYSKSTSLSTKLSALIIK"
     CDS             10881..11756
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt5"
                     /product="glycosyltransferase"
                     /protein_id="AXY99701.1"
                     /translation="MNISIIICCYNSEARIKNTLKALYSCIDNYAYECEVIIVDNNST
                     DKTREIALEYWESINKNNYRMNIFFESTPGLSYARYCGAINSNYDLLLFCDDDNWLQK
                     TYFHSIPDYFKNKNVGALGGRGIPFSDKPIPDWFFEHMDAYACSTQKKGMLYGASLVI
                     RKDILMNFYSNDLSKSIIGRTAEKLDSGEDNFLCEYITSSGYTLESNNIPFIHFMSSA
                     RLTPEYLYKLSYSFGQATYKIRSQNKGKYFHTLYITLTIIKEIYLSFFCCKYKKIRLQ
                     RGISFIKGYLASWIN"
     CDS             11874..13040
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99702.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNQKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKNIDVLFTDSTEAEAIKLFANTYLALRVSYFNELDSYA
                     QAYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEDTFFNSKLIRDLDAFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     CDS             13052..13555
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXY99703.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(13643..14197)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemX"
                     /product="wemX"
                     /protein_id="AXY99704.1"
                     /translation="MHAQAKVTLAELKECLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGDHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGEGCRYITKVIADIKQV"
     CDS             complement(14200..15033)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99705.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPMLASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(15133..16146)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99706.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDLIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
     gene            complement(16146..16307)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5946 a   2499 c   2420 g   5442 t
ORIGIN
        1 cgcaccccac taactcgttt acagctggcc gtgcattact acgtcgtcgt agcggtgaaa
       61 gtaaagagct agagcgtatt gaaacagaaa cacagcggct agatggcatg atcaatgact
      121 tattagtgct ttcccgcaat caatataaaa atgagttatt acgcgaaacc gtaaaagcga
      181 atgagctttg gaacgatatt ttagataatg cgaaatttga ggctgaacaa agcaataaaa
      241 ccttaacagt aaccgcccca ccaggcccat ggacgattta ttgcaatcct tattcattag
      301 cgagtgcctt tgaaaatatc gttcgcaatg ccttgcgtta ttcacattct cgtattgaag
      361 tggcctttac cgaacaaaat cagggtatca ctattattgt tgatgatgat ggcccagggg
      421 ttagcccaga agatcgcgag catattttcc gaccttttta tcgtacggac gaagcacgtg
      481 atagagaatc cggcggtaca gggctgggac tggcaattgt tgaaaccgca attagtcaac
      541 atcgaggcca tgtaaaagcc gatgatagcc cattaggggg attaagagta gagatttggt
      601 tgccgagagg gagtgtggta aataagtcaa ataataatta actgtaccat aagccactaa
      661 gttaaaactt ttaccttctt tttatgcagt ggcttattat tttttagtaa ataaatctat
      721 aataacagta gttaaactca ttagctcctt tcgcataata caaacaaaaa tattaatgca
      781 ttttaacacc ttatttatta atcctttttt gaaagtcact cctcatattt ttatatccta
      841 gtctttaaga ttaatatgtt tagatgatgt ataatactag taatattata aactctgggt
      901 actttcccag tgcggtagct atgcctattg gcttttagag aaaatactta tatggaaaca
      961 atcacagtaa ctagcccatt atttccaccg ttggaagagc tagaacctta cttacaagat
     1021 atatggaaga gaaaatggct aaccaataat ggttactatc atcaacgtct tgaagaggca
     1081 ctatgtaaat acctaaatgt accttacatt agtttatatt caaacggaac attagcgcta
     1141 attgcagcaa taaacgtctt aaagctgaaa ggtgaagtaa tcacaacgcc atatagcttt
     1201 gtcgcaacta gtcattcatt actacatagt catattacgc ctgtctttgt agatatagag
     1261 ccaaatacct ataatataga tcccgataaa attgaagcag caattaccga taagacaacc
     1321 gcgattttac ctgttcatgt atatggtcgc ccatgtaatc atgaagcgat tcaaaaaatt
     1381 gcagataagt ataaccttcc cgtgatttat gatgctgccc acgcctttgg ggtaaaagaa
     1441 aatggtaact ccatattaaa tcaaggtaac ttatctattt taagttttca tgctactaag
     1501 acttactcta caattgaagg cggagcgatc atttgccata gcgcagaaat gaaagaaaaa
     1561 ttagataaat ataaaaattt tggttacaca aatgaaacag aaatcgatga actaggattc
     1621 aatgcgaagc tcaatgaagt acaagctgca tttggtttgc taacattaaa ttatatagat
     1681 aaagcaatag aaaaaagaaa agaagtttct gatttttata aaactaatct aagcaatgta
     1741 cctggtgtta cattacctaa tataccaaaa gacttaactt ggaattattc ttactatcct
     1801 ataataatag ataaagacag gtatggtaaa acaagagatg aactttattt agagctaaaa
     1861 gaaaataaaa tattaagccg tagatatttt tacccattaa taactgattt taatatttat
     1921 aatcaatata aaacaaaaac ccctaccgct gaaaaaatat ctgaaaatgt tatttgttta
     1981 ccaatccatc atttattaag taatgatgac ttaaatagaa taattaatgt tattaaatat
     2041 gactataaat aaactaatta atcccttacc agaaaaagaa ataataaaaa aatgggattc
     2101 tgaagaagtt ttcgtaagtg tgatatgttg tacatataac caagaaaaat atatagaaga
     2161 tacaatactt ggagtactct gccaagatac gccttataga tatgaattta ttatacatga
     2221 tgattgttct actgattcta catcagaaat aataaaaaaa tatgcagata attatccgaa
     2281 aataataaaa catattattc ccgctaagaa tatttattca caaggaattc agcctgggat
     2341 taatgctttt tctttttcta atggtaaata cattgcttta tgtgaaggag atgatttttg
     2401 gatagatgag aagaagttaa caaagcaggt cgatatatta gaaaataaaa aagatatcaa
     2461 catttgtttc agctcagctt atacacttca acctaatgga aaaattgatc tcacatgtaa
     2521 ctatcataac cagatcttct gtgctcctct ttccactgtt atccgtcaag gtggggcata
     2581 tatgccaacc gcatctcttc ttttcagaag agatgtattt atacagttta aaaataattt
     2641 ttcaaaagct tcgattggtg attattattg ccaaatatta ggtagtataa ataagggagc
     2701 tatttattta cctgatatta catgtgtata tcgtatcaat tcattaggct cttggtctaa
     2761 aagtaggata aatgtcagta aagagatttt aatttctgaa atagatgaat atgaaagaaa
     2821 tctttataaa ttaattatat taggtgctga taaaaaagat atcgattatt cgttagcaga
     2881 tatctgtagg gtaaatgcaa atgaattatt tcttagaaaa gattggagcg gttgtaaatc
     2941 tctaattatt aaatcctgga tttttaataa aaaaataaat agaaatcaag aaaaactata
     3001 tttatataga aatatattac ccatcttata tttaagagaa aaaataaaaa aatcactaaa
     3061 aaaaatcagt ctaaaaagga ttaagttata atgagattag cagtaatgca accttacctt
     3121 ttcccatata ttggttatta tcaactagct tactatgctg ataaatttat attttatgat
     3181 gatgttaact atatcaaagg aggatatata aacagaaaca atatattaac taaaaatgga
     3241 ttacagatgt ttactcttcc tgtgaacaaa gcatctagct ttaagaagat aaatgaactt
     3301 tactttcaag ataatactaa aaaaatatta acatcaatat atcagaatta ttctaaaaca
     3361 gcctattttc attctatttt tcctataatt gaaaatgtct taaccaataa taataggaat
     3421 gttgcaaata tcacatcatt atcaataatt aagatatttg aatacctttc attaaaattt
     3481 aattatacat tctcatcaga tcaaaactat aacagaacgt ataacgccca agagaaaata
     3541 tatgagctat gcaaaataaa tgatgctcag gaatatataa atccaattgg tggaaaagac
     3601 ctttacagca aaaatgaatt taaaaaaaga aatataaaat taaatttctt agaacctata
     3661 ctatctccct atactcaaat aaattcatct aattttgtaa gtaacacatc agtgattgat
     3721 attctaatga attgtaataa agatgaaata ataaatcaat ttaacaattt taaaattata
     3781 taggtctaag tatgtcatac tacacaacag aacaattagc agaaattggt tttaagaaga
     3841 ttggaacaaa ttgtaagata agtactaagg catctatata tcgaccagaa gaaattgaaa
     3901 tagggaatga ttgtcgtatt gatgactttg ttattttatc aggaaaaata cacttaaaaa
     3961 ataatattca cattgcagca ttttgtaatt taagtgcagg tgaatgtagc atcctatttg
     4021 aagatttttc agggctagcc tatggatgcc atgtctttac aggcagtgat gactatagcg
     4081 gtagaacaat gacaaatcca acaattcccc tgaaatataa aaatctaaaa gaaaaaaata
     4141 ttattatagg aaagcatgca atcatcggaa cgaactcaat cattatgcct ggcgttacca
     4201 tagcagaagg aaccgctgta ggcgctctat ccatggttac caaaagcaca aaaccatggg
     4261 gaatatattt tggaaaccca gctaaaagaa taaaagaaag aaaaaaagat ttattaaatt
     4321 tagaaatgga atatataaaa gaaaaatcaa aaaaatagac ttaattacaa taagttaaaa
     4381 tcattttgta ctttaatgag gctatttata caagtattag ttatgccacc attaaccatc
     4441 aatataaggc catatattgc ttagtaaatt caataaaata aaaccaacaa ccactattaa
     4501 aacctatcac actatattat tcttatagta gaattttcta taaatcaaaa taaaaaagac
     4561 gtttacatca acatattaag gtacaagtac taattatgtg gtttttatgt taagtcatct
     4621 tattagataa cgagtcacat attcttttta tccacatctt tatagatatt tcagaatgat
     4681 cttgctaaat cagtcaatat tccgatactt atatacctaa tcaatgttag aatacccacc
     4741 aattgcttga tccgctggtt tgaagagtct aatctcagag cggtttggca tactgttaaa
     4801 gtatgcttat ataacaaaac tcttatatta cagatagata agaggttttc gagaaatttt
     4861 tggcgctctg ggggccgtaa acccagtgcg gtagccatga gtaaactaat gaaaatagta
     4921 tctttcacca ttgtttggta tctcatcttt ttcttaattg ctatatctgg tttaacaggg
     4981 ttacagactc cttcacaaat agcaataacg agtattggac ttattttatc atcctatctt
     5041 attggttgta ttactagttt tctattaccc agaactaaaa agaatgttta tgaaacaaaa
     5101 aattataata aaaaacgtaa gaataacttt gaatatatta taaaaatatt tctattttac
     5161 tttatctctc actctttatt tatcataata aatgtatttt taataaataa aatagatatt
     5221 aataactata gagagtcatt ttttgatggc tcacgaaaaa atttaatctt ttttggtggg
     5281 tggtggttgt tctacttgta ttacctatcg actttatttc tattagcgtt agtaccattt
     5341 tttgttgcaa caaaaaaaat aaatattcaa atcttattat tcatatcggt tatattatat
     5401 gatattgttt ttctttctag aacagggatt tattattttt tattagcgac tatttgtgcc
     5461 gttattatta gagaattaaa acttaaatta atatttttac ttgtcgtagt gattataatg
     5521 ttgtcactca ctattagcta ttttagaggt gatatttcaa atatatttat cttaataaaa
     5581 aatgccatta tcaattatca tgttgcacct tacattttgt ttgataaaaa cataatatca
     5641 gaaaacacta tacgttataa tggcttagga ttagcatcac taggtatata taatattttt
     5701 ctttatattt ttgataataa tgttatggaa aatataaata atctgagggc taatctaaat
     5761 gtgttttatg atttaagcag taatgataat tatatacctt ataatgctta ttatacttcc
     5821 ttaggtttaa tttatattga ttttggatta ataggttgta ttataatttc ttatttttct
     5881 ggattattta tatcttactt ggcaataaag agtaaaaact ccgaaaaata taaaatttca
     5941 tgtgttttta ttagttcgct ttatttagag tctttatttt caccaatcac aataaatatt
     6001 ttctcatttg tgatagttgt gtttatctta ttactaatga tacataatta tgaatatagc
     6061 aattgtagtc ccgtctttaa gaaaaactgc tccagtaaat atagcaatta gcatttataa
     6121 ggatataaaa agctattgta acgtccacgc cacgttgttt tatcttgata aaataaaaga
     6181 tattgataat aatataaata atatccctga agccatttta ttaaatagaa aaaattataa
     6241 attacttaaa aattttgata tcgtccacac ccatgggtta cgacctgata ttatcagttt
     6301 tttgtatgca aaaaaaaagt caagagtaac aactctgcat agcatgatta taaaagactt
     6361 gatttctcgt tatggaatta aaggtttttt tatttcatat atgtggattt ttatcttaat
     6421 attctttaat aaaagagtat ctattagcaa tagtgtaaaa aaacatttaa taaagaaaaa
     6481 aataagttca acagtcatat acaatgggat taaatttgat agttttaata taaaaaatga
     6541 taaagaatac cttaaaatag attgttatat aaattcaata aaaaaaaata aaattgttgt
     6601 aggtactatt agccatatag aaaaaataaa aggtatagaa caattaatag aattagctaa
     6661 attaaatgaa aatatttttt taataataat tggagatgga acatataaat acaaaataga
     6721 gaaacttgtt tctttatata atttagaaaa taggatttta tttactggtc atataaatca
     6781 taatgctcaa tattactcta agttatttga tgtttatgta caaccatcga taaatgaagg
     6841 ctttggtctt tctgtaattg aagctatttg caataaaatt cctgtagtat gttcaaatat
     6901 tgaagttttt actgaattat tcaataaaca acaaattagt ttctttcaat taaatgatat
     6961 aaactcacta aacaatgcaa tacaaaaagc aacaactaaa aataccacag aattagaaac
     7021 tattgctaat actataaaag atagattttc agtatcaact atgtctaaaa attatattaa
     7081 cttatatgaa actattataa aaaatgaaag ataaattaat aatttttctc tgttatggtg
     7141 ttaatttcat tatacctatt ttatttttta gatttattag tgagcgttta gatgatataa
     7201 atctagcaac attttttttt catttggcga ttatttttta ttttcaatat gttattgagt
     7261 atggatttca agtatcttta ataaaatttt tattcaaaat aaaaaacaac aaaaaaaaaa
     7321 taaacataat tatatcttct gttatttatt ttagattatt tcttttttta ttagcattcc
     7381 taattgtgag tttgtttttt tatattttgg tttatgataa gagtttttta tatatttacc
     7441 tgattttgat tggaaatatt ttttcatttc aatttctata tcagattttt aatgaattaa
     7501 aaatctatta cattttatca ttattttcta aattattatt tctacctcta atctttatct
     7561 ctgacaatat aactacttta tatatacttt ttaatataat acctaattta atactttttg
     7621 cttttataat aaaaaaatat aaaatatttt tcttaatact taatatacag attatattta
     7681 agatagctaa aaataaattt tcttatttcg catctaattt atctataact ttatatacta
     7741 atttcaatca aataattctt ggtctttttc atccattaac gctcccagcc tatgcattag
     7801 cagataaaat catcagatct atcgtggctg gaaattatat ttttatacaa atatgccaaa
     7861 taaaatttct agataatgct aatttaaaaa ataagaaatt gaggaataaa tttatcactt
     7921 tattctttat attaggtata gttgaattat tatttttagt ttcgctaagc tatccaataa
     7981 ataaatacct ttttcctaat atacttaata ttaatacctt tattattata ttatcacttt
     8041 tattacctat tattttaatg agcaatctat atggtatggt ttttttgact tgctcaaaca
     8101 aaacaaagct tttatcaaga gtattcttta gtgctgcctc tattgcaatt atgacatctc
     8161 catttcttat atatttatat gatggatttg gagcactaat cgctagcatt attgcagaac
     8221 taatagtact cattttatgt atgaaatatt actctactat ttttggaaat agaaatgcct
     8281 aatgttgcta taattatgtc tatttatgca aatgatattt tatctcaagt taaagaagct
     8341 atagatagta ttttttccca aaattataat aatataagaa tctttatatg tattgatggt
     8401 gaaattaatc aagatataat gaattattta aattctttga aaaaaatatt tcttataaaa
     8461 agggataaaa ataaaggtct agcaatttca ttaaatgaac ttattgaatg tgttcttaaa
     8521 gaagatagat tcaaatatat agctagaatg gatgccgacg atatatcaat gcctgataga
     8581 atacaaaaac aagtccagta tcttgaaaaa aatttagata tatctgtttt aggaaccaaa
     8641 tgtaaagagt ttggtagcat tcatgcccgt gaatattgtt ctgtatatga atctaataat
     8701 gatatttaca aatatatttt taaaaaatgc cctttcgttc atccatcagt tatgtttaga
     8761 actgaagtat ttaaatcagg tattagatat cctacaaatg aaccttttac cgaagatcta
     8821 gctttatggt ttattttagc tatgaaaaaa tataaattct ctaacctaaa tgaatgttta
     8881 ttaaaattta gaataaatga tcaaacagtc tcaagaagaa gaggatataa aaaagcaatt
     8941 attgaattta aaattcgtct aaaatatcta atattattga aacgaataaa taatattcat
     9001 gatatttatt atactcttgg tcattttttt gtcaggctat tacctacccc gttgtttaag
     9061 agaatttata taaaaggtta ataaatgaaa aaaatattat atagctatga tatttgggat
     9121 actattttaa aaagacactg cccccccaaa gatacaataa gaatttctgt ttatatattc
     9181 cttttatatt atgggataac tcaacatctt aatgatattt tggaaaatat atttcaaaag
     9241 gaaaatgaat attttaataa aaataaagaa tattatattt ttgatttact ttatcaagaa
     9301 gtaaaaaaaa ttaaaaatct agatttaaca aatatagata agaaacaatt aaaaaaaata
     9361 tgggataata tatatataaa tgtagaaaaa aaatcaactt atccaaataa aaaaatttta
     9421 gaactaaaag acaaggataa aggagaaaaa atatacatct cagattttta tatagaaaaa
     9481 aaacttttag ataatatctt aaaccaccat aatatatatt ttaatgatgg ttttgtatca
     9541 tgcgatgaaa acaaaaacaa gtattcaggt gacttatacg aaaagataaa aaatatatat
     9601 catactaatt ctattaattg ggttcatact ggtgataata aaatatctga tataaaagtt
     9661 gcaagatcct taaaaataaa aactcgatat gttaataaat ttaacttaag caataaaata
     9721 aaaaaattat tcccagtaaa aaaacaatat aacgacatag aaaatctatc tattattcta
     9781 atagggttta gtaaatttat tctggaagaa tgcaaaaaaa ataaagtaaa tgatatttat
     9841 ttttttacaa gagaaggtgt tacttttaaa agattttttg acttattttt aaaagaaata
     9901 tcttctaatt ttaatataaa ctttaatatt aagacacata ttgtctatgt tagcagaact
     9961 gcgactatac cactacgatt agatatcaat gatagttatt taggatttaa agatgctata
    10021 ttacaatatg ggaatgatac caacacattt ttaagttttt ttaatctatc taaaaaatta
    10081 gcacatttat ctgaaaaata taaaaaaatt gaggaactat taaaaaatga aaaagataaa
    10141 agaatattaa tagaattaat atcaaataaa aaaaatttag caataaacta tcttgaatca
    10201 ttaggatttt ttgagaaaaa taaattaata gttgatatcg gttggagagg tagcatacaa
    10261 gataatctgt cacatataga taaaaaaata aaattaggtt gctatttagg tttatttgaa
    10321 ttttttcaaa atcaaaataa tgctaaaaaa gagggatata tttttaataa taatactaat
    10381 aaagaaagta tttttcacaa gggtgtggat ttttgggaaa ctatttttaa tacaaaagga
    10441 ggtagtgtaa cttcatatgt taataatata cctaaaaaga aaatcaacaa agatgaagag
    10501 ctatttttag atgaagtaat taatgtacaa gaaataattt acaatgaagc gaaacatcga
    10561 ttgcatgata ttataaagaa taaattatca ttattagata ttgataacca tgcaagactt
    10621 gcttataaaa aaatatcaac tagaccatca tcttatttag ctgatttata tctttcatct
    10681 ctacaaaatg aaaactatgg attaaattca tttaagagaa aatatataaa tatcacgata
    10741 aaatctttaa ttaaatcatt ttttaataaa aaagagaaag aatctatttc taatttaatt
    10801 aaaaacaatg gatggagaga atctattttg tattcaaaat caacatcctt atcaaccaaa
    10861 ctatctgcgt taatcataaa atgaatatat caataataat ttgctgttat aatagtgaag
    10921 ctagaataaa aaatacatta aaagcactct atagctgtat tgataattac gcatatgaat
    10981 gtgaagtaat tatcgttgat aataattcta cagataaaac aagagaaata gctttagaat
    11041 attgggagag tattaataaa aataattata gaatgaatat attttttgaa tctactcctg
    11101 ggcttagtta cgctagatac tgtggggcca taaattccaa ttatgatttg ttattatttt
    11161 gtgatgatga taattggtta caaaaaacat attttcatag catacctgat tattttaaaa
    11221 ataagaatgt tggagcttta ggtggacgag gtattccttt ttcagataag ccaatacctg
    11281 attggttttt tgaacatatg gacgcttatg catgttccac acaaaaaaaa ggaatgttat
    11341 atggcgcaag tctagttata agaaaagata ttttaatgaa tttctattcc aatgacctat
    11401 ctaaaagcat tataggtagg acagcagaaa aattggactc tggtgaagat aattttctat
    11461 gtgaatatat aactagtagc gggtatactt tagaaagtaa taatataccg tttatacatt
    11521 ttatgtcatc agcacgatta acacctgaat atttatataa gttatcatat tcatttgggc
    11581 aggctacata taaaataaga tcccaaaaca agggtaaata cttccatact ctttatataa
    11641 cattaaccat aataaaggaa atttatttat catttttttg ctgtaaatat aaaaaaatca
    11701 gacttcaaag aggaatctct tttattaaag gttatttagc tagctggatt aattaacgac
    11761 attattaacg tctaatttaa atgataatat tattttttag gctataaatt caatagtcca
    11821 aattactatc tataatatac atataaattt agattaatca tcaaggtcta tttatgaaaa
    11881 tcaccatctc aggaaccggc tatgtcggtc tatccaacgg gatactcctt tcccagcaca
    11941 ataaagttgt tgcattagat attaataaag aaaaagtcga actattaaac caaaaaatct
    12001 ctcctatctc tgataaagaa attgaagagt ttttagccac taaagagctc gattttacgg
    12061 caactttaga taaagaattc gcttataaag atgctgaatt tgttatcatt gccactccta
    12121 ccgattatga tcctaaaact aactacttta acacctcttc tgtagagtcg gttattcgtg
    12181 atgttttaga atataatcca aacactacta tgattataaa atcaaccatt cctgttggtt
    12241 ttactaaaca gatgagagaa aaatatcata ccgataatat tatcttctct cctgagttct
    12301 tacgagaagg tcgcgccctt tacgataacc tttatccatc tcgtattgtg attggtgagc
    12361 aatctgcaag agcagagaaa tttgctgaac ttttacaaca aggggcatta aaaaaaaata
    12421 ttgatgttct ttttactgac tctacagaag cagaagcaat caaactattt gccaatactt
    12481 atctcgcact gcgtgtttct tattttaatg aactcgatag ctacgcccaa gcttacggtc
    12541 taaacgcaag acaaattatt gaaggtgtct gtttagatcc gcgcattggt aatcactata
    12601 ataacccctc atttggttat ggcggttact gcttaccaaa agatactaaa caactgttag
    12661 ccaactacga atctgtacct aataatatca ttggtgccat tgtggaagct aaccgtactc
    12721 gtaaagactt tattgctgat tctattatcg ctaaatcacc gaagattgtt ggtgtttatc
    12781 gtttaattat gaaatcaggc tctgataatt tccgctcatc ttcaattcaa ggcattatga
    12841 agcgtattaa agcgaaaggc attgaagtgg taatttatga gccagaaatg aaagaagata
    12901 ctttctttaa ctcaaaactc attcgtgatt tagatgcgtt taaagcaatg tctgatgtca
    12961 ttattaccaa tagaatggct tctgcactaa acgatgttga agaaaaagtt tatactcgtg
    13021 atctctttgg taacgattaa aactaattta tatgctcaat atcgtcttat ttgaacccga
    13081 aattccaccg aatacgggta atattatccg cctgtgtgcc aacacaggct ttcaccttca
    13141 tttgatccaa ccattgggtt ttacttggga tgataaacgg ctacgccgtg cagggcttga
    13201 ttatcgtgag tttgctgata tcaaacagca tcacgattat tatgcttttt tagaaagtga
    13261 aggattaagc cctgataatg cacaatcccc gacaggtgca cggttgtttg cattaaccac
    13321 taaaggtact cccgcccata gtaatgtaag ttatcaagat ggggattatc tgctatttgg
    13381 ccctgagaca cgaggtttac caccttatgt gttggataat atgccaacac aacaaaaaat
    13441 ccgtgtacca atgttagcgg atagtcgcag tatgaatctt tctaatacgg ttgccgtggt
    13501 tgtttttgag gcatggcgcc aattaggcta tccgggtgcg ttattaagag attgatttag
    13561 tggttagttt ggttaattaa agttatttta attcatactc attacttgat tttatcttta
    13621 tttataaacc tttgttcaag cattatacct gcttgatatc cgcaatcact ttcgtgatat
    13681 accgacaccc ttcccctaca tcctttaata ctaatgcatg ggcgccaata atcgcattat
    13741 caccaatatt aatctctcca ccaataatgg atgaattaca gccaatataa acatgatctc
    13801 ctacttggat attggctttt ccccctttct cttttacgcc aatagtcacc ccttgcataa
    13861 tagtgacata ttcccccaat tgacagtaat tagtcagtac aatgccaatc gggtgtggta
    13921 aagaaaggcc tttaccaata cgggttttta acgagatatc attacagtat ttacggttat
    13981 tgctagcacc gatccgaata cctaatttat gtaaaaagcg atggggagag aaataaaaat
    14041 attgtgctac ccgccaccaa aataggtaac gggaacgctg acagaaaaac actcgatgaa
    14101 acagtcttaa ccaactaaag ggttttttat caccaatata ttcagcctgt aagcactctt
    14161 ttagttcagc aagggtaacc ttagcttggg catgcatagt taaataccat caccacactc
    14221 aaagccgggt acaacaccat taaagtgttg atccatatcg aatgacggtt tttcactatc
    14281 aggacgaccg acaatgcggg caggcacccc ggccaccgta gtatgagctg gtacttcatg
    14341 taatactacc gagccagcac caattttagc accacgacca atctcgatat taccaagaat
    14401 tttagctcct gcgccaatca ttaccccttc acgcactttc ggatgacgat cgcccccagt
    14461 tttacctgtt ccccctaagg tcacagattg taaaatggag acatcatttt caaccaccgc
    14521 cgtttcacca attacaatac ctgtcgcatg gtcgagcata ataccgtgtc caatacgtgc
    14581 cgctgggtga atatccaccc caaaggtgac tgatatttgg ttttgtagat aaatggctag
    14641 cgcttggcga ttttgttgcc atagccaatg ggcaatacgg taggcttgta atgcatgaaa
    14701 cccttttaaa tagagcaagg gagtagaata tttatctacc gcgggatcac gtaggcgtac
    14761 cgcggagaga tcacaagcgg ccgactcaat cattgagcta tcgtgactat aagcctcttc
    14821 aacgatttcg cgtactgcaa tggctggcat agtctgagtt gccagcttat tggccagcat
    14881 atagcttaat gcactaccta aattttcgtg tttgagaagt gtcgcatgaa agaaactggc
    14941 taacattggc tcacaatctg ccaatgcacg cgcttcactt ttaatatgat cccagactct
    15001 tgttagttct tctagcgaca tactcactct cattttttac accaaagcct aagtaaatac
    15061 ttaggctttg aattttcaaa caaatggata gttaaaacag ataccctaag aatattattt
    15121 aaaataaact gactacaggc tgctgtctat ctcatctttg gttgctcgcc ctaataaagc
    15181 caatgcagcc tcttttacat ctttatgttg atataaaatt tggtaaatct gttcggtgat
    15241 tggcatttct acacccactt gttcggctaa tgcgcgaact tctttggtgt tacgataacc
    15301 ttcgactacc tggccaattt tctcttgggc tgtatcaaca tctaaacctt gacctagcat
    15361 cataccaaag cgacgattgc gtgattggtt gtcagtacag gttaaaacca aatcacccaa
    15421 accagccatg cccataaaag ttgccgcatc tgcacctaag gctttaccta agcggctcat
    15481 ttcggccaga ccacgggtaa tcagtgcagt acgcgcattc gcgccaaatc ccataccatc
    15541 agacataccc gcaccaatag caatcacgtt tttaacagcg ccccccagtt gcacaccgat
    15601 taaatcaggg tttttataga ctcggaagct tttaccacaa tgaaatagct gttgtaactg
    15661 ctctaaaaag agattgtctg tcgaggccac tgcaatcgcg gtgggcaaac ccgccgcaag
    15721 ctctttagca aaagtagggc cagataatac tgcgagcggg atttcattac ctaatacttc
    15781 acgggcaaca tcttgtaata agcgaccagt atgtgcttca agaccttttg tcgcccaaac
    15841 aacacgtgca tcctgacgta aaaagggttt gatttgttgc agtacttcac caaacacatg
    15901 gcttgggatc accacaagaa tattacggct cgcttcaatc gctttttgca aagaagcttc
    15961 catatataaa ctatcaggaa aggaaacatc gggcagaaag gcttgattac agcgcgcttg
    16021 ttctaatgcc gcaacgtgct tgggatcatg cccccacagc acaacatcgt gcccattacg
    16081 cgctaaggta atcgctaaag cggtgccgta tgaaccggca ccgataactg tcatagaagc
    16141 gttcattaag cttcttcaac tcctgagttt gcagcatcct gttgctgttg ttgctgtaag
    16201 tagttcataa acaacgcatc aagttaacag gtgctaagtt taattgtggg aaagtaccac
    16261 gacccactaa gttggtgata cattcacgtg cataagggaa taatacg
//
