LOCUS       KY710711               16993 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus vulgaris strain PrK 63/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710711
VERSION     KY710711.1
KEYWORDS    .
SOURCE      Proteus vulgaris
  ORGANISM  Proteus vulgaris
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 16993)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16993)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16993
                     /organism="Proteus vulgaris"
                     /mol_type="genomic DNA"
                     /strain="PrK 63/57"
                     /serotype="O37ab"
                     /db_xref="taxon:585"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..16993
                     /note="O antigen gene cluster"
     gene            77..610
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             922..1971
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99707.1"
                     /translation="MKKKSILIYGEYSGYGKSLAKGFVELGHNAEIFSFQGDGFKNIQ
                     SGITLQGNNNKERKKNIFKLIPKFLEFKNIIIVNTEFVNRKNNGFLITLLFKLTRKRI
                     ILLACGDDTPFIKYGKKGLIPNWPYEDISLPNKKYLIRIKDYILTHYIASIAYKIIPC
                     MYDYSMSWKQTCFKYKVTETIPLACDGKLEPIKKTGDKVVIMHGINRIDFKGTKVVKA
                     ALSEIEKEYPNDVEVIYPEHLPLTEYLEVMKRVDIAIDQTKGNSYGMNAIYSMFSGHI
                     VLAPANDLFLNDINLQDSPVIKINNDKDSIVNSLSLLLKEKNMLDDKKKASQEYAVNI
                     HSPIKIAAKIENYLI"
     CDS             1984..2865
                     /note="glucose-1-phosphate-thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="rmlA"
                     /product="rmlA"
                     /protein_id="AXY99708.1"
                     /translation="MKGIILAGGSGTRLSPITMGTSKQLLPIYDKPMIYYPLSVLMLA
                     GINDILIITTPEDQNNFYRLLGNGSQFGISLTFAVQPKPEGLAQAFIIGEKFIGNDSV
                     CLVLGDNIFWGQGFSPKLINAANKKNGATIFGYEVQDPERFGVVEFDDNLNATSIEEK
                     PKKPKSNFAVTGLYFYDNRVIDIAKNIEPSERGELEITTINQTYLQENKLSVELLGRG
                     FAWLDTGTHDSLLDAGIFVETIEKRQGFKIACLEEIAYKQKWLTEEQIYNIANKYKKN
                     SYGQYLLKLIKKENEIS"
     CDS             2852..3247
                     /note="dTDP-6-deoxy-3,4-keto-hexulose isomerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="qdtA"
                     /product="qdtA"
                     /protein_id="AXY99709.1"
                     /translation="MKLVNIIDFNILGDERGSLISLEENNNIPFEIKRVYYIFGTKDG
                     VSRGFHAHKELKQLAVCVRGSCRFIMDDGTKREELILDKPNLGLLINPMQWHEMHDFS
                     EDCIIIVLANDYYDESDYIRDYDSFIKSE"
     CDS             3264..3785
                     /note="orf05"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf05"
                     /product="butyryltransferase"
                     /protein_id="AXY99710.1"
                     /translation="MILTSKTIKLRLVNEDDAEFILSLRTNSTYNRFLSPVNNDIKKQ
                     KEWIKFYKKKEKNNEEFYFIIEKLDNTPCGTVRIYDIHANSFSWGSWILNKNKTRTAA
                     VESALLVYIFGFERKKFSRCHFEVMKGNEKVISFHEKFNATRVNEDENHYFYEIEPKN
                     IIDIKNKFINFIK"
     CDS             3792..4895
                     /note="DegT/DnrJ/EryC1/StrS aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="qdtB"
                     /product="qdtB"
                     /protein_id="AXY99711.1"
                     /translation="MISFLDLKLINNQHNDELKEACARVIDSGWYIMGKELENFENNF
                     SNYCGTKHTIGVANGLDALTLVLRAWKELGKLKSGDEVIVQANTYIASILAITENNLV
                     PILVEPDSKTYNLSIKNIKSVITDRTKAILPVHLYGQISPMKEIMEFAKENDLLVLED
                     CAQSQGASIDGIKAGNWGHAAGFSFYPGKNLGALGDAGAITTSDNKLAETVFALRNYG
                     SQKKYKNIYQGVNSRLDEMQAAILSVKLKYLDEENKYRRKIANYYLKNINNEKLKVRN
                     KNNSEEHVWHLFVIECNERDRLSEYLTSCGVQTLIHYPTPPHKQEAYKDLGITTPLPL
                     TEEIHNKILSLPISPILSFEDVKFIVQKLNEFK"
     CDS             5372..6640
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99712.1"
                     /translation="MNKLITNIIWMLFEKITTIVGGIIITIYVARYLGPNDMGIINYA
                     LALIAILIPIAQLGADKLFFNIIVKSSKSGVNIIHATKFNRLIIYSILSILITIYFYK
                     YKSENISSLVLFLGIIISGLFQAADIYKIYYDATLQSKINSIVTQFSLWTSLLSRLIL
                     VKISAPLLFFSLPYILNSAIPYFIKSKIFNKKNNNTLNKKHKKKYSHYATKAGIPLAV
                     SSISIIMYTRLNQIILAEYESLYDVGIYNAGLTLAQGWIFIPIAISTSLFAKVLKMEK
                     KSKLNGYSFIFFINIISSTPIIILYFIFYNEIILFTFGIDFIQSESVILILTITTLFS
                     AIGGSASRVIIAENGYIFIMYKTIILTIINIILGITLISNYGLIGAAYTSLITEILGC
                     IILNILFKKGLIILLIIKSIKNINFYKKLL"
     CDS             6637..7911
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99713.1"
                     /translation="MIETFLFSLIVIISSLFIIIIYPKNTNKGILAVLLWQLSWLLFS
                     TINPLELKFPPRNSIFFIILCSLSFIFGFIQNKTQLIRNSNNQRLNIEENIKVIVPIS
                     IILIIIYIILSLKAIIMMQNVPIYEYRRMIYQNPEELFFIKQGLPIFQFLIEGLTLLL
                     TIKGFYLLLFKKRKKIFITVIIISILSSIVFLGRYQLYRVALLFIFYFLLFEKNKNKK
                     LMAMIIFLFFVILLFSFSLFRGHGVYGITEIFIKHILGYHTFGYHLFEKYLIDIDFID
                     KHSWFGLASLGSFGYLISKPFSFFGFETYLQSLQFTSQDVFINLGIVDWKNIVHIEIL
                     ANAFYTGFRELFTDFNYFSIVFFYPIGILSRYCYKRFLLNDYIGIQCIMLIMFLILFF
                     GMKNPFNRHEIIIPVVYYIFYLYKTRFNFRYV"
     CDS             7904..8788
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99714.1"
                     /translation="MYKISVICTVLNRIHDLERLTSSLINQKTDVEYEIILIDNGSTD
                     GTAEYVSILSSSYSNIKYIDAKIFLGSPYSARNMGIKLASGEYLCFIDGYANENWVET
                     INRTANNNTITAGKIIIEVTKTCSIYEIYDSIFNLDNEKIVNKYHRAPTGNLVIHRKI
                     FDKLGSFNESIRSGGDMIFTSLAYKNGYKINYNENQISYYYARDKNAILKKQTRISRG
                     QINIWKSENKTTQYLLKSIIKIFIISNPIKIFSYIEKRKIIPLSFTQKLHLYLINEKM
                     KYIMILNNLKEFITQLGK"
     CDS             8790..10337
                     /note="orf10"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="acyltransferase"
                     /protein_id="AXY99715.1"
                     /translation="MSKLQYRPELDGIRAISILLVLLFHIDKNFIPGGFIGVDIFFVL
                     SGFLISKIIIIGIQNHTFSYNDFYKRRIKRILPPFYFMVLLTVISSFFIFLPSDINQL
                     IKSAISTLIFSSNYFFSGGLDYFSSNADEQPLLHTWSLAVEEQFYFLFPILIYFLIRI
                     KNRNKLYLLLLCIFSFLIAEILSNIDATKSFSYFSLPTRMGELLIGTLCALTTIKLKH
                     TLFQYIGLGLIIFSSFFIDEKTAFPGIFAFIPCLGAGLLILSENKDTTLFKILSNSIV
                     TYIGKLSFSLYLFHWPILAFSRYITGEYSLIQNNSIYIIILIIIFISIFSYEFVENKV
                     RYSKLNFKKSFIYIYLIPSLLIILLTFFYWTGKLQFNEYSKDITSYDNNLNICHNKFL
                     NNCYLGNGSIKVALFGDSHAAQISYFLLHKESDYSFYLNSASSCPIFDNIDLSHITSN
                     SIYNRCIDLRNNIKEEINKFDIIFVSQKWDQYLLDKDNIKKIEQWLAKYKDKKNYFFY
                     YNHQSIN"
     CDS             10591..11634
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99716.1"
                     /translation="MRKIISIDFNSLDHMGGTSTFNKNLSKIFSREIYFITYYKFKNE
                     PTDNELNLGIKKNLFFKILNFISSYRLSSFLIKRKIPKETDILILNSPSFLRFKLPGK
                     KKILILHQDIDVMINNRSNFSGSKHFFYKVLNEINYFIIPSEEDKIKLINKYNVDGGK
                     LLVIPHLSKVSILHTNSLKKGTKKLIMICRLYNKQKRIDLVLNAMSHLKDWQLTIVGD
                     GPDSEYLKNIKEKNKLDNVSFYGPSDNISELLDQHSIHIMSSDYEGFGLTNIEAISRG
                     LPIIIRNTFPAAKIIINDSGVLLNKNWNQDEFIKALEKIYNNYDYYSKNALENARKYS
                     FEQVKSAWDNLLN"
     CDS             11662..12828
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99717.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVIALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKELAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKEMRDKYNTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSERAQKFADLLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESYGLNAKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYDSVPNNIISAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSRLVRDLNEFKSISDVIITNRMSSLLNDVEEKVYTRDLFGND"
     CDS             12863..13654
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt4"
                     /product="glycosyltransferase"
                     /protein_id="AXY99718.1"
                     /translation="MLSKNTNFLISIIMPSYNSGEYIRKSIDSVLRQSYKNLELIIID
                     DCSNDNTIEVISSYKDERIKILKNESNQGAGITRNKGLSLANGRFICFLDSDDYWRDD
                     KLEKQLNFMLSNNIDFCFSAYDRVSEDDAFICKIGVPEKVNYSDMLKVCSIGCLTVMF
                     DKDKFTDFSMPSIRKRQDYALWLKLLKQTPYAYGISESLGSYNIRENSISSNKLDAAK
                     HQWRIYRDIENLNIFSASYYFSHYAFNGFFRSKLPSLAKKLGLLK"
     CDS             13687..14190
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenM"
                     /product="wenM"
                     /protein_id="AXY99719.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLNPDNAQSPTGARVFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(14262..14819)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wenH"
                     /product="wenH"
                     /protein_id="AXY99720.1"
                     /translation="MALTEVTLSELKACLHAEYIGDNKPFSWVRLFHRVFFCQRSRYL
                     FWWRVAQFFYFSKSRFLKKLGIRIGAKNNRKYCNDISLRTRIGKGLSLPHPIGIVLTN
                     YCQLGENVTLMQGVTIGVKEKDGKADIRVGNHVYIGCNSSIIGGEIEIGDNAVIGAHA
                     LVLKDVGEGCRYITKVVAEIRQLSE"
     CDS             complement(14819..15640)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99721.1"
                     /translation="MSLEELKRVWDHIKDEARCLADCEPILASFFHATLLKHENLGSA
                     LSYMLANKLATQTMPAIAVREIVEEAYRSDNSMIESAAYDISAVRLRDPAVDKYSTPL
                     LYLKGFHALQAYRIAHWLWNQDRKALAVYLQNQISVTFGVDIHPAARIGHGIMLDHAT
                     GIVIGETAIVENDVSILQSVTLGGTGKTCGDRHPKVREGVMIGAGAKILGNIEIGRGA
                     KIGAGSVVLHEVPAHTTVAGVPARIVGKPTSDKPSLDMDQHFNGVVPGFECGDGI"
     CDS             complement(15730..16746)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99722.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPEHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGDVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAISVASTDNTF
                     SEELQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGFDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDAKEAALSLLGRATKDEID
                     SNLF"
     gene            complement(16746..16991)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     6150 a   2643 c   2595 g   5605 t
ORIGIN
        1 cgcactgtta cgtcgtcgta gtggagaaag taaagaacta gaacgcattg aaacagaaac
       61 tcaacgcctt gatggcatga ttaatgattt actagttctt tcacgcaacc aacataaaaa
      121 cgagttatta cgcgaaacgg taaaagccaa cgagctgtgg gatgacattt tagataacgc
      181 taaatttgag gctgaacaaa gtaataaaac gctacaagtg acaacgcctc caggagcatg
      241 gcctatctac tgcaatccat attcacttgc tagtgcattt gaaaatattg ttcgtaatgc
      301 cctacgttat tcacattctc gcattgaggt tgcatttact gaacaaaacc aaggtatcac
      361 aattattgtt gatgatgatg gcccaggtgt tagtcctgaa gatagagagc atatcttccg
      421 acctttctat cgcactgacg aagcaagaga tcgtgaatca ggtggtactg gcttgggatt
      481 agctattgtt gagacagcag taagccaaca tagaggccat gttaaagccg atgacagccc
      541 attaggtgga ttgagagttg agatttggtt gccgagggct ggagctggta ataaatcacc
      601 aacagcttaa tagagtaatt aaactgttgt atcaaaatat actgccatct attgaagaat
      661 aggtggcata ctttttaaaa atatccagta tataaaaaaa caacaaaata aaaatataat
      721 ttatctctat tcaattaatg ctaaaatatc tatgaataag taacattaat aaattaaaaa
      781 taacaatata caataaaaac aactagataa atggattaaa ttcaaatttg gcactctgga
      841 agccataaat tcagtgcggt agctatgcta aaaattagtt tctacttgct aagttaaata
      901 ttacaaacaa aagttataca gatgaaaaaa aaatcaatac ttatttatgg tgaatatagt
      961 ggctacggaa aatcacttgc taaaggattt gtagaattag gacataatgc agaaattttt
     1021 agttttcaag gtgatgggtt taaaaatatt caatcaggta ttactctaca aggaaacaac
     1081 aataaagaaa gaaaaaaaaa tatttttaaa ctaatcccta agttcttaga atttaaaaat
     1141 ataatcattg taaatactga gtttgtaaat agaaaaaaca acggttttct aattacttta
     1201 ttattcaaat taacaagaaa aagaataata cttcttgctt gtggtgatga caccccattt
     1261 ataaaatatg gtaaaaaagg attaattcct aactggccat atgaagatat ttcattacca
     1321 aataaaaaat atttaatacg aattaaagat tatattctaa cacattatat tgcaagcata
     1381 gcttataaaa ttataccttg tatgtacgac tatagtatga gttggaaaca aacttgtttt
     1441 aaatataaag tgacagaaac tattcctctt gcatgtgatg gaaaactaga gcccataaaa
     1501 aaaacaggag ataaagttgt tatcatgcat ggaataaatc gtatagattt taaaggaaca
     1561 aaagtagtta aagctgctct tagtgaaata gaaaaggaat atcctaatga tgttgaagtt
     1621 atctaccctg aacatttacc tttaactgaa tatttagagg taatgaagcg agtagatatt
     1681 gcaatagatc aaactaaagg aaattcttat gggatgaatg ctatttatag catgttttct
     1741 ggtcatattg ttttagctcc ggccaacgat ttattcctca atgatataaa ccttcaagat
     1801 tccccagtta ttaagatcaa taatgacaaa gacagtattg tgaactcatt aagtttatta
     1861 ctaaaagaaa aaaacatgct tgatgataaa aaaaaggcat ctcaagagta tgcggttaac
     1921 atacactctc caataaaaat agcagcaaaa atagaaaatt atctaattta aaaagggata
     1981 acaatgaaag gtattatatt agcaggaggt tcaggtacta gattatctcc tattacaatg
     2041 ggaacatcga aacaattact ccccatttat gataaaccaa tgatttatta tcctctatca
     2101 gtgctaatgc ttgctggcat taacgatata ttaataatca caactcctga agatcaaaat
     2161 aatttttata gattgttagg taatggttca caatttggta tttccctaac ctttgcggta
     2221 caacccaagc ctgaaggatt ggctcaagca tttattattg gtgaaaaatt tattggtaat
     2281 gacagtgtat gtttggtttt aggggataat atattttggg gacaaggctt ctcaccaaaa
     2341 ctaataaatg cagctaataa gaaaaatggt gctactattt ttggctatga ggttcaagat
     2401 cctgaaagat ttggagttgt tgaatttgat gataatttaa atgcaacttc tattgaagaa
     2461 aaacctaaaa aaccaaaatc taattttgcc gttactggtt tatattttta tgataaccga
     2521 gttatcgata ttgctaaaaa tatagaacca tctgaacgag gtgaattaga aataacaact
     2581 attaatcaaa catatctaca agaaaacaaa ttgagcgttg agctattagg tcgtggcttt
     2641 gcatggttag atacaggaac tcatgatagt ttacttgatg ctggaatttt tgttgagaca
     2701 attgaaaaaa ggcaaggatt caaaattgct tgtttagaag aaatagcata caaacaaaaa
     2761 tggttaactg aagaacaaat atacaatata gctaataaat acaagaaaaa tagctatggt
     2821 caatacttat taaaattaat aaagaaagaa aatgaaatta gttaatatta tagattttaa
     2881 tatacttgga gatgaaagag gttcccttat ctccttagaa gaaaataaca acatcccatt
     2941 tgagataaaa cgagtatatt atatttttgg tacgaaagat ggagtatcta gaggatttca
     3001 cgctcataaa gagctcaaac aattggctgt ttgtgttcgt ggtagttgcc gttttattat
     3061 ggatgatggc acaaaaagag aagaactaat tctagataag cccaacttag ggttattaat
     3121 taatcctatg caatggcatg aaatgcatga tttttcagaa gattgcataa taattgttct
     3181 cgctaatgat tactatgatg aaagtgatta tatacgtgat tatgactctt ttataaaatc
     3241 cgaataaatt ataggtgagc aatatgatat taacatctaa gactataaaa ttaaggcttg
     3301 taaatgaaga tgatgctgag tttatattat ccttaagaac taatagcacc tataatagat
     3361 ttttatctcc tgtaaacaac gatattaaaa aacaaaaaga atggataaaa ttttataaaa
     3421 agaaagaaaa aaataacgaa gaattttatt ttataataga aaaattagat aatactcctt
     3481 gtggcaccgt aagaatctac gatattcatg ctaattcatt ttcatgggga agctggatat
     3541 taaataaaaa taaaactcga accgctgcag tcgaaagtgc actattagtc tatatatttg
     3601 gttttgaaag aaaaaaattc tctcgttgtc atttcgaagt gatgaaagga aatgaaaaag
     3661 ttatttcttt ccatgaaaaa tttaatgcta caagagtaaa tgaagatgaa aatcattatt
     3721 tttatgaaat agagccaaaa aatattatag atataaaaaa taaatttata aacttcataa
     3781 agtaggcaac catgatctct ttcttagatt taaaattaat aaataatcaa cataatgatg
     3841 aactaaaaga agcctgtgct agagtcattg attctggctg gtatatcatg ggaaaagaat
     3901 tagaaaattt tgaaaacaat ttttctaatt attgtggtac taaacatact attggtgttg
     3961 caaatggttt agatgcttta actttagttt taagggcatg gaaagaattg ggtaaactaa
     4021 aatcaggtga tgaagttatt gttcaggcga acacctacat agcatctata ttagcaatta
     4081 cagaaaataa tttagttcct attcttgttg aacctgatag caaaacatat aacctatcaa
     4141 ttaagaatat aaagtcagta attaccgata gaactaaagc tattttacca gttcaccttt
     4201 atgggcaaat tagcccaatg aaagaaatta tggaatttgc aaaagaaaat gacctattag
     4261 ttctagaaga ttgtgctcaa tcacaaggag catccataga tggaataaaa gctggtaatt
     4321 ggggccatgc tgcaggtttt agcttttatc caggaaaaaa cttaggagct ttgggtgatg
     4381 ctggtgcaat tacaactagt gataacaaac ttgctgaaac agtttttgca ttacgcaact
     4441 atggctcaca aaaaaaatac aaaaatatat atcaaggtgt aaatagccgt ttagatgaaa
     4501 tgcaagctgc aatattaagt gtcaaactga aatatttaga tgaggaaaat aaatatagaa
     4561 gaaaaattgc aaattattat ctaaaaaata tcaataatga aaaactgaag gttagaaata
     4621 agaataactc agaagaacat gtttggcatc tttttgttat tgagtgtaat gaaagagata
     4681 ggttatctga atatttaaca tcatgtggag ttcaaacact aatccattat ccaacacctc
     4741 cacacaagca agaagcttat aaagacttag gaattacaac accactccca ttaacagaag
     4801 aaattcataa taaaattctc tcacttccta tttcaccgat actatcattt gaggacgtta
     4861 agtttattgt tcaaaaatta aacgaattta aataatcttt atattatata attacttatt
     4921 tttcaatatg atatcatata aattgtattt ataattatac aattttcaaa ttgactaaga
     4981 aaacaacaaa atattgagta attaaactgt tgtatcaaaa tatactgcca cctattgaag
     5041 aataggtggc atattctttt tgttagcatt tgctgatgta attcagaatg atctggctga
     5101 atcgatggtt attccagtat ttatatatct tcaatcaatg ctaaaatacc cagctattac
     5161 ttgaccgtct ggtttgaaga gtctactctc agtgcggttt ttcgcatatt gttaaagtat
     5221 gcttatataa taaaactctt atattacaaa aagataagag tttttcgaaa taaattttgg
     5281 cgctctgggg gccgtaaacc cagtgcggta gctatggtag tattaactat aataaacata
     5341 tcttataaac tagtcattaa tttaaaatga aatgaataaa cttattacta atattatatg
     5401 gatgttattt gaaaaaataa caactatcgt tggtggtata ataataacaa tttatgtagc
     5461 gagatatctt ggtcccaatg acatgggtat tatcaattat gccttagcat tgatcgctat
     5521 tttaattcct attgcacaat taggtgcgga taaacttttt tttaatatca tagttaaatc
     5581 atcaaagtca ggagttaata taatacatgc caccaaattt aatagactca ttatttatag
     5641 tatattatct attttaataa caatatattt ttataaatat aaatcagaaa atatatcatc
     5701 tctagttttg tttcttggta taattattag cgggttattt caagcagctg atatatataa
     5761 aatatattat gatgctactc ttcaatcaaa aataaactct atagttacac aattttcttt
     5821 atggactagt cttctatcac gcttaattct cgtaaaaata agtgcgccat tattattttt
     5881 ttcattacct tacattctaa actctgcaat tccatatttt ataaagtcaa aaatattcaa
     5941 caaaaagaac aacaacacac taaataagaa acacaaaaaa aaatatagtc attatgcaac
     6001 taaagccgga ataccattag cagtaagttc tatttctata attatgtaca cccgattaaa
     6061 tcaaataata ttagcggaat atgaaagttt atatgatgtt ggcatttata atgcgggttt
     6121 aaccctggct caaggttgga ttttcattcc aattgcaata agtacttcat tgtttgcaaa
     6181 agtattaaaa atggaaaaaa aatcaaaatt aaatggctat agttttattt tttttataaa
     6241 tattatctca tctaccccta ttattatttt atattttata ttttataatg aaattattct
     6301 ttttactttc ggtatcgatt ttattcaatc agaaagtgtc attttaattt taacgatcac
     6361 cactctattt tcagcaatag gaggctcagc atctcgagta attatagcgg agaatggata
     6421 tattttcatt atgtacaaaa caattattct aacaataatt aacataatac taggaataac
     6481 acttatatca aattatggtc taattggcgc agcatataca tcacttatta cagaaatact
     6541 aggctgtatt atattaaaca ttctttttaa gaaaggtttg ataattctgc taataataaa
     6601 atcaatcaaa aatataaatt tttataaaaa actattatga tagaaacatt tttattttct
     6661 ctaattgtta ttatttcaag cttatttata attataatat atcctaagaa tacgaataaa
     6721 ggaatattag ccgttttact atggcaatta agttggctac tattttcgac catcaaccct
     6781 ctagaactca aattcccgcc aagaaattct atttttttta ttattctttg tagtttatct
     6841 tttatatttg ggttcataca aaacaaaaca cagcttataa gaaatagtaa caaccaaaga
     6901 ttaaatatag aagaaaatat aaaagtaata gtccccatta gcattatcct catcataatt
     6961 tatattattc tgtcattaaa agccattata atgatgcaaa acgtgcctat atatgaatat
     7021 agaagaatga tttatcaaaa cccagaggaa ttattcttca taaaacaagg tttacctata
     7081 ttccaatttt taattgaggg tttaaccctt ttattaacaa taaaagggtt ctatttatta
     7141 ctatttaaaa aaagaaaaaa aatattcata actgtaatta tcatttcaat attatcatct
     7201 attgtatttc ttggtagata tcaactttat agagtcgctt tgctatttat cttttatttt
     7261 cttctatttg aaaaaaataa aaataaaaaa ttaatggcca tgattatatt tttgtttttc
     7321 gtaattttac tattttcgtt ttctcttttt agaggtcatg gtgtatacgg aataactgag
     7381 attttcatta aacatatttt aggctatcat acttttggat atcatttatt tgagaaatac
     7441 ttaattgata tagattttat tgataagcac tcatggtttg ggttagcatc actaggtagt
     7501 tttggatatc taatttcaaa accattctca ttcttcggat ttgaaaccta ccttcaatcc
     7561 ttacaattca catcacaaga tgtgtttatt aatttaggta ttgttgattg gaaaaacatt
     7621 gttcatattg aaatattggc caatgcattt tatacaggat tccgcgaatt atttactgat
     7681 tttaactatt ttagtatagt gtttttttat cctataggta ttttaagtcg atattgttat
     7741 aaacgttttc tcttaaatga ctatattgga atacagtgca ttatgttaat tatgttttta
     7801 attctttttt tcggtatgaa aaatcctttt aatagacatg aaattataat accggttgtt
     7861 tattatattt tttatttata taaaacaagg tttaatttta gatatgtata aaatatctgt
     7921 tatatgtaca gtattaaata gaatacatga tttagaacga cttacgtcaa gcttaattaa
     7981 tcaaaaaact gatgtagagt acgaaattat actaatagat aatggttcta ctgatggcac
     8041 agctgaatat gtaagcattc taagttcatc gtattctaat ataaaatata ttgatgcaaa
     8101 aatctttttg gggagccctt actctgctag aaatatgggt ataaaattag cttcaggtga
     8161 atatctttgt tttattgatg gttatgctaa tgagaactgg gtagaaacta taaatagaac
     8221 agcaaataac aatactatta cagcagggaa aataattatt gaagtaacta aaacatgctc
     8281 aatatatgaa atatacgata gtatttttaa tctagataat gaaaaaattg ttaacaaata
     8341 tcatagagcc cccactggta atttagtcat tcatagaaaa atatttgata aattaggatc
     8401 ttttaatgaa agtattcgtt ctggtggaga tatgatattt acatcattag cctataagaa
     8461 cggctataaa ataaattata atgaaaacca gataagttat tattacgcta gagataaaaa
     8521 tgccatatta aaaaagcaaa ctagaatatc aagaggtcaa ataaatatct ggaaatcaga
     8581 aaacaaaacg actcaatatc ttttaaaatc cataataaaa atattcataa taagcaatcc
     8641 cattaaaata ttttcatata tagaaaaaag gaaaataata ccattatctt tcacacaaaa
     8701 attacactta tatttaataa atgaaaaaat gaaatacata atgatattaa acaatctaaa
     8761 agaatttatt acacagcttg gaaaataata tgagtaaatt acaatataga cccgaattag
     8821 atgggatcag ggctatctct atattattgg ttttattatt tcatatagat aaaaatttta
     8881 ttccaggtgg atttattggc gttgatatat tctttgtatt atcaggtttt ttaatttcta
     8941 aaattataat aattggaata cagaatcata ctttttctta taatgatttt tataaaagaa
     9001 gaataaaaag aatactccct cctttttatt ttatggtttt attgacagta atatcatctt
     9061 tttttatttt tcttccctca gatattaatc agttgataaa atctgctatt agtactctta
     9121 ttttctcatc aaattacttt ttttctggtg gattagatta cttctcctca aatgcagatg
     9181 aacaaccatt attgcatact tggtcattag cggtcgaaga acagttctat tttttattcc
     9241 caatattaat atattttctt attagaataa aaaatagaaa taaattatac ttattactac
     9301 tatgtatatt ttctttttta atcgctgaaa tattatctaa tattgatgct acaaaatcat
     9361 tttcatattt tagtttacca acaagaatgg gagaacttct tattggaaca ctatgtgctt
     9421 taactacaat taaattaaaa catactttat ttcaatatat aggattagga ttaataatat
     9481 tctcatcatt ttttattgat gaaaaaacgg catttcctgg tatatttgca tttattcctt
     9541 gtttgggcgc aggactatta atattatccg aaaataaaga caccacccta tttaaaattt
     9601 tgtcaaatag tatagtgacg tatattggta aattatcttt ctcattatat ttatttcatt
     9661 ggcctatctt agccttcagt agatatatta ctggtgaata ttcattaata caaaataata
     9721 gtatatatat tattatttta ataattatat tcatttctat tttttcatat gaatttgtag
     9781 aaaataaagt tagatattcc aagcttaact ttaaaaagtc ctttatctat atttatttaa
     9841 tcccatcact ccttataata ctattaacat tcttctattg gacaggaaaa cttcaattta
     9901 atgaatatag taaagatata acaagttatg ataataattt aaatatatgt cataataaat
     9961 ttttaaataa ctgttatcta ggtaacggct caataaaagt tgcattgttt ggtgactctc
    10021 atgctgctca aataagttat tttttacttc ataaagaaag tgactattca ttttatttaa
    10081 atagtgcaag ttcttgccct atatttgata acattgacct ctctcacatt acaagtaatt
    10141 caatttacaa tagatgtatt gatttgagaa acaatataaa agaagagata aataagtttg
    10201 atatcatttt tgtttctcaa aaatgggatc aatatctttt agataaagat aatataaaaa
    10261 aaatagaaca atggttagcc aagtataaag ataaaaaaaa ttattttttt tattacaacc
    10321 accagagtat aaactagatg tccttaggag ttataaattt aatttgataa atgaaaaaga
    10381 caataattat ttactagcta ataatatatt aataaatata gccaaaaaat atgacaatgc
    10441 ttattattta tctttatata acgcattgga gaatattgat aacggtatat acaataacat
    10501 tccattatat agagacaacc atcacattaa tattttaggt tcaaaatact tacttgaaca
    10561 cactaatagt attcaagagg agatcaaacc atgagaaaaa taattagtat tgattttaac
    10621 tctcttgatc atatgggagg aacttcaacc tttaataaaa atctttctaa gatattttct
    10681 cgtgaaattt atttcattac atattataaa tttaaaaatg aacctaccga taatgaatta
    10741 aacctgggaa taaagaagaa tttatttttt aaaatattaa actttatatc atcatatcgt
    10801 ttatctagtt ttcttataaa gagaaaaatt ccaaaagaaa ctgacatttt aattcttaac
    10861 tcaccatctt ttctgagatt taaacttcct ggcaaaaaaa aaatcctaat tttacatcaa
    10921 gatatagatg taatgataaa taacagatct aatttttccg gctcaaaaca ttttttttat
    10981 aaagttctaa atgaaataaa ctatttcatt attccttctg aggaagataa aatcaaatta
    11041 attaataaat ataatgttga tggaggaaaa ttattagtca tcccacactt atcaaaagtg
    11101 tcaatattac atactaattc ccttaaaaag ggaaccaaga aacttattat gatatgtaga
    11161 ctatataaca aacaaaaaag aatagattta gtcttaaatg ccatgtctca tttaaaagat
    11221 tggcaattaa caatcgttgg cgatgggcct gattctgaat atttaaaaaa tataaaagaa
    11281 aaaaataagc ttgataatgt tagtttttat ggtccatccg ataatatatc tgaactatta
    11341 gatcaacatt ctattcacat tatgtctagt gattatgaag gttttggttt aacgaatata
    11401 gaagctattt ctagaggatt accaataata attcgaaata cctttcctgc agctaaaata
    11461 attattaatg attcaggagt tctccttaat aaaaactgga atcaggatga atttatcaag
    11521 gctttagaaa aaatttataa taattatgat tattactcaa agaatgctct agaaaatgcc
    11581 cgtaaatact cctttgaaca agtgaagtca gcttgggata atttattaaa ttaacattac
    11641 aatttgaata ggttatgtat aatgaaaatc accatctctg gtaccggcta tgtcggtcta
    11701 tctaacggca tacttctttc ccaaaacaac aaagttattg cgttagatat caataaagaa
    11761 aaagtcgaac tcttaaacaa aaaaatctca cctatttctg ataaagaaat tgaagagttt
    11821 ttggcaacca aagagctcga ttttaccgca accttagata aagaactcgc ttataaagat
    11881 gccgaatttg ttatcattgc cacacctacc gattacgatc ctaaaacaaa ctactttaat
    11941 acctcttcag tagagtctgt tatccgtgat gttttagaat ataatccaaa tacgacaatg
    12001 attataaaat caaccattcc tgttggtttt acgaaagaaa tgagagataa atataataca
    12061 gataatatta ttttctcccc agaattctta cgtgaaggtc gtgcacttta cgataattta
    12121 tatccatctc gtattgtgat tggtgaacaa tcagaaagag cacaaaaatt tgcagacctt
    12181 ttacaacaag gtgcactgaa aaaagatatt gatgttctgt tcactgattc aacagaagca
    12241 gaagctataa aattatttgc gaatacttat ttagcactac gtgttgctta ctttaatgag
    12301 cttgatagtt atgcagaatc ttatggctta aatgcaaaac aaatcattga aggtgtctgt
    12361 ttagatccgc gtattggcaa ccattataat aatccatcgt ttggttatgg cggttactgc
    12421 ttaccaaaag acaccaagca actgttagca aactacgatt ctgtccctaa taatattatt
    12481 agtgccatcg ttgaagctaa ccgcacacgt aaagacttta ttgcagactc aattatcgct
    12541 aaatcaccaa aaatcgtcgg tgtttatcgt ttaattatga aatctggttc tgataatttc
    12601 cgctcttctt ctatccaagg cattatgaaa cgtattaaag ccaaagggat tgaagtggtg
    12661 atttatgagc cagagatgaa agaagaaacc ttctttaact caagattagt tcgtgattta
    12721 aatgagttta aatcaatctc tgatgttatt attaccaata gaatgtcatc tttactaaac
    12781 gatgttgaag aaaaagttta tactcgtgac ctatttggta acgattaatt atatattttt
    12841 ctttaattag gtcgtagttc taatgttatc caaaaatact aattttttaa tatcaatcat
    12901 tatgccatcc tataattcag gagaatatat acggaagtct atagattcag ttttgagaca
    12961 atcctataaa aatcttgaat taataattat tgatgattgc tctaatgata ataccattga
    13021 agtaatatcc tcttataaag atgagaggat taaaatatta aaaaatgaat ctaatcaagg
    13081 tgctggaatt actcgtaata aaggattatc acttgctaat gggcgtttta tttgtttttt
    13141 agatagtgat gattattggc gagatgataa gttagaaaaa cagcttaatt ttatgctatc
    13201 taataacata gatttttgtt tttctgctta tgatagagtt tctgaggatg acgcttttat
    13261 ctgtaagatc ggtgtccctg aaaaagtaaa ctactcagac atgctaaaag tttgttctat
    13321 tggttgctta acagtaatgt ttgataaaga caaatttact gatttttcaa tgccatcaat
    13381 aaggaaaaga caggattatg ctctctggtt aaagctattg aaacaaacac cttacgccta
    13441 tggaataagc gaatctctcg gttcttataa tataagagaa aattcaatct cctccaataa
    13501 acttgacgca gcaaagcacc aatggcggat ttatcgagat atagagaatc ttaatatatt
    13561 ttcagcttcc tattattttt ctcactatgc ttttaatggt ttttttagat ctaaacttcc
    13621 ttctttagct aaaaaactgg gtcttttgaa ataatattag tttctacatc ttgtcttagg
    13681 ctttatatgc tcaatatcgt cttatttgaa cccgaaattc caccgaatac gggtaatatt
    13741 attcgcctgt gtgctaacac aggctttcat cttcatctaa tccaaccatt gggttttact
    13801 tgggatgata aacgtctacg ccgtgcaggg cttgattacc gtgaatttgc tgatattaaa
    13861 cagcatcacg attactatgc ttttttagaa agtgaaggtt taaaccctga taatgcacaa
    13921 tcaccaacag gtgcacgtgt atttgcgtta acgacaaaag gtacaccagc acatagtaat
    13981 gtgagctatc aagatggaga ttatctcctt tttggacctg aaactcgtgg tttaccgcct
    14041 tacgtgttgg ataatatgcc gactcagcag aaaatcagag ttccaatgct tgctgatagc
    14101 cgtagtatga acctatcaaa tactgtagcg gttgtggttt ttgaagcatg gcgtcaactt
    14161 ggttatcctg gtgcattact aagagactga aatgcaaaaa gcgctgatca taaataatga
    14221 ctagcgcttt tttaatttca aatgactaaa aatctaactt attactctga cagttgtctg
    14281 atctcagcaa caactttagt aatataccta cacccttccc ccacatcctt taataccaat
    14341 gcgtgagcac caatcactgc attatcacct atctctatct ctccgccgat aatcgaagaa
    14401 ttacagccaa tataaacatg gttgccaact ctaatatctg ctttgccatc tttttctttt
    14461 actccaatgg tgacaccttg cattaacgtc acgttctcac ctaattggca gtaattggtc
    14521 agtacaatcc ctatgggatg aggtaatgaa aggcctttac ctatacgagt tcttagtgaa
    14581 atatcgttgc agtacttgcg attattttta gcaccaatac gaatgccaag ttttttgagg
    14641 aaacgacttt tagagaaata aaagaattgg gcgacacgcc accaaaatag ataacgtgaa
    14701 cgttggcaga aaaagacgcg atgaaatagt ctcacccaac taaaaggctt gttatcaccg
    14761 atatattcag catgtaaaca agccttcagc tcagagagtg taacttcagt taatgccatt
    14821 aaatgccatc accacattca aaaccgggca ccacaccatt aaagtgttga tccatatcga
    14881 gtgaaggctt gtcactcgtt ggcttaccaa caatgcgagc aggaactccc gcaactgtag
    14941 tgtgcgcagg aacttcatgt aaaacaactg atcccgcgcc aattttagca ccacggccaa
    15001 tctcaatatt gccgagtatt ttagcacccg ctccaatcat tacaccttcg cgtactttag
    15061 ggtggcgatc accacaggtt ttacctgtcc cccctaaagt gacggattgt aaaatagaaa
    15121 cgtcattctc aacaatggcg gtttcaccaa tcacaatacc tgttgcgtga tcaagcataa
    15181 tgccatgacc aatgcgagcg gcgggatgaa tatcaacccc aaaagtcaca gaaatttgat
    15241 tttgcagata aacggcaagc gctttacgat cttggttcca taaccaatgc gctatgcgat
    15301 aagcttgtaa ggcatgaaaa ccttttagat aaagcagagg tgtagagtac ttatcaacag
    15361 cgggatcgcg tagacgaacg gctgaaatat cgtatgccgc agattcaatc atagagttat
    15421 cactacgata ggcttcttca acaatctcac gaacagcaat cgcaggcata gtctgagttg
    15481 ccagcttgtt cgccagcata tagcttaagg cactgcctaa gttttcatgc ttgagtaatg
    15541 tcgcatggaa gaaactcgcc aaaatcggct cacaatctgc caaacatctt gcttcatctt
    15601 taatatgatc ccagactctt tttagttctt ctagcgacat actcactctc actcttttta
    15661 cattgaagcc taagtaaaaa cttaggcttc ttatgtttaa aacagaaata ataactatct
    15721 aaaatagact tagaaaagat tgctgtctat ctcatctttc gttgcacggc ctaataaaga
    15781 taatgcagct tctttggcat ctttatgctg gtataaaatt tggtagatct gctctgtgat
    15841 aggcatttca acaccaactt gttctgctaa tgcgcgaact tctttagtat tgcgataacc
    15901 ttcaacgact tgcccaattt tttcttgagc ggtatcgaca tcaaaacctt ggcctagcat
    15961 cataccaaat cgacgattac gagattggtt atcagtacag gttaaaacta aatcacctaa
    16021 acccgccatt cccataaacg ttgccgcatc tgcacctaat gctttaccta aacggctcat
    16081 ttcggcaagt ccacgggtga tcagcgctgt acgcgcattc gcaccaaaac ccataccatc
    16141 agacataccc gcaccaatcg caatcacgtt ttttaccgca ccacctaatt gcacaccgat
    16201 aaagtcagga tttttataaa cacggaaact tttgccacaa tggaaaagct gttgaagctc
    16261 ttcagagaac gtattatccg ttgatgccac agaaatcgca gtgggtaaac cagcggcaag
    16321 ctctttagca aaagtagggc ctgataaaac tgctagcggg atttcattac ctaatacttc
    16381 acgcgctaca tcttgcaaca agcgaccagt atgggcctca agtccttttg tcgcccaaac
    16441 aacacgtgca tcctgacgta aaaagggttt gatctgttgt aatacatcac caaaaacatg
    16501 gcttgggatc acaacaagaa tattacggct ggcttcaatg gctttttgca aagaagcttc
    16561 catgtataaa ctatcaggaa aggaaacatc gggcagaaat gcctgattac agcgtgcttg
    16621 ttctaacgct gcaacatgct caggatcatg accccaaagt acgacgtcat gaccattgcg
    16681 cgctaaggta atcgctaaag cggtgccgta tgaaccggca ccgataactg tcatagaagc
    16741 gttcattaag cttcttcagc cccttcatta gcagcatcag tttgttgctg ttgtaaatag
    16801 ttcataaaca acgcatcaaa gttaacaggt gctaagttca gttgtgggaa agtaccacgg
    16861 ctaactaagt tagtgataca ttcacgagcg taagggaaca gaacgttagg gcaatatgca
    16921 cctaagcaat gtgccagttg agtgccttcg ataccttcaa cggtaaagat acctgcttgt
    16981 tgtacttcac aca
//
