LOCUS       GU254059               16197 bp    DNA     linear   BCT 14-AUG-2010
DEFINITION  Proteus mirabilis strain CCUG 4637 O antigen gene cluster, complete
            sequence; and WemG (wemG), WemH (wemH), CysE (cysE), and GpsA
            (gpsA) genes, complete cds.
ACCESSION   GU254059
VERSION     GU254059.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 16197)
  AUTHORS   Wang,Q., Torzewska,A., Ruan,X., Wang,X., Rozalski,A., Shao,Z.,
            Guo,X., Zhou,H., Feng,L. and Wang,L.
  TITLE     Molecular and genetic analyses of the putative Proteus O antigen
            gene locus
  JOURNAL   Appl. Environ. Microbiol. 76 (16), 5471-5478 (2010)
   PUBMED   20581173
REFERENCE   2  (bases 1 to 16197)
  AUTHORS   Wang,Q., Torzewska,A., Ruan,X., Rozalski,A., Guo,X., Shao,Z.,
            Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..16197
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="CCUG 4637"
                     /db_xref="taxon:584"
                     /note="serogroup: O3a,3b"
     gene            569..1714
                     /gene="glf"
     CDS             569..1714
                     /gene="glf"
                     /note="UDP-galactopyranose mutase"
                     /codon_start=1
                     /transl_table=11
                     /product="Glf"
                     /protein_id="ADL32275.1"
                     /translation="MKFLVVGCGLTGAVISRHLADAGHYITIIDQRSHIASNCHTERD
                     RNTGIMEHVYGPHIFHTSDEVVWEYINKHGEMIPYVNRVKTTYQNQVYSLPINLHTIN
                     QFYQKALNPEQAKTFLSTITDKSITDPKNFEEQALKFIGKDLYEAFFKGYTVKQWGCD
                     PKNLPASILKRLPVRFTYDDNYYSHKYQGMPKHGYTDIVRSILDHKNIQVITNKKYDH
                     SEREEYDHIIWTGKIDEWFGYCEGRLNYRTLDFIKHETIGDYQGTAVMNYCDIAVPYT
                     RIHEHKHFSPWESHDKTIYIEEHSRICEENDIPYYPIHLEEENKILNKYLELAQKEKY
                     ITFAGRLGTYRYMDMDVSIKEALMLAENLVSDLIDQNELKSFYHKHQ"
     misc_feature    569..12492
                     /note="O antigen gene cluster"
     gene            1729..2487
                     /gene="wemA"
     CDS             1729..2487
                     /gene="wemA"
                     /codon_start=1
                     /transl_table=11
                     /product="WemA"
                     /protein_id="ADL32276.1"
                     /translation="MSKFLAVIRCGDNSLHKNWVNDNAQFDVILSYFGEDIPYSLDNI
                     KYVHHFKGSKWEGLYDLFHNHPELWADYDYIWLPDDDLDSTVENINLFFELMQKYQFD
                     LCQPALTNNSYYSYKDLLQEPDLIYRETNFVEVMAPCFSKKIISKAYQTFNENKSGWG
                     LDFYWPILFKEENIKVGVIDSTPIHHTRPVGIAGHGSSDKTLSPLNELHQLLDKYSLE
                     IPTFVATSLLTQKGKMIQKGSFSYSLRKFMKKRK"
     gene            2969..3871
                     /gene="wemB"
     CDS             2969..3871
                     /gene="wemB"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32277.1"
                     /translation="MMNEYIKHIRKKVLRSLCNSFPTLVTKLLYYRRFGKRLNLKQPK
                     TFNEKLQWLKLNTYKNNLLVTQCADKYKVREYIKKTHCEDILNDIYYTWNSPLEINWE
                     ALPNKFVLKCNHGAGYNIVCRDKEKLNQDKIITTLTKWMKEDYWKNSVELCYKDIPKK
                     IICEKFIETESKELPLDYKVFCFHGKAEFVMICTDRESQKPKFFFVDKDWNLLPYGLD
                     YKYITDASILTKPIGYEKLFFYAEKLSKPFPFVRADFYLNDNNILFGELTFTPAAGLD
                     IELNNKEIRNVDIIIGNLLNLNRI"
     gene            3874..5094
                     /gene="wzx"
     CDS             3874..5094
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ADL32278.1"
                     /translation="MELTKKKEILSNLVSLSSINILGILIPIITMPILSRSLGIDTYG
                     EYLLFMTILIFGHTITDYSVQYIGVRQASNHKYNNIKLSVIYINYQTLRLFLGSVYFL
                     LSLSYSICFLNVHFTYWILYGGSLYLIGYVLTSAWFYLSIGNTKILIISSLFTKLINL
                     LIIIFFIKKSDDIDLLILSTTLPLFISGFLLYLNIKLKFKLKFIFRAKNIYRYIIKGK
                     NVFLGILSPNLYNSLPIIILGSISTPSEFSKFAIATRIIGLITSFQDIFARSVFPILS
                     REKKNHIITLLKINSLFSVLAVVFILCFGEYFLYLFLGKDYANNIYLNILSISIIFIG
                     FMNSFSDGFFLPKKYDLIYRNISIRISLISCTISFILILKFGILGGCIGITTARMLFS
                     IDYFRTYYNLSKKV"
     gene            5096..6199
                     /gene="wemC"
     CDS             5096..6199
                     /gene="wemC"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32279.1"
                     /translation="MNILILLPSLGGGGAEKNAVLIANQLAKKNNVHIVIIYNEITNE
                     NRKRLDNNVSLTLLNKKKIIFSMWKIRNIILKENYDVILTTVAYFGITFSFLLPFINR
                     KKKIYIYRETNIPSIYNKNFNILKRIIFKLGYLYTMKNYDYIICQSNDMINDLNIYSS
                     IPSRKIIKINNPITRPECHPSIINNKKSENYVFAAGRLTQQKGFLDLIKNYSTSNALK
                     KNIKLYIAGMGPQYNEMKILINSLNLNANIILLGFKDDLSSYLLNAKAFILSSNYEGF
                     PNIVLEALSYGCPVLSNDCPGGINEIIIPNFNGLIYSKNNFNEMLDLILKIDFDRNQI
                     SEDIIKRYDANLLLSKYDEIIDNDSNINFNHNK"
     gene            6165..6983
                     /gene="wemD"
     CDS             6165..6983
                     /gene="wemD"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32280.1"
                     /translation="MIAISISTTTNRLFNIKKEQFPQQEGIIYFISIQGLDIDKNKVG
                     LFIKNNLPYFRVLYTESKGLSNNRNNSLKELYKINTVEYIYIADDDIYVNIKGIFQLV
                     EKMKLNDISLGCGKISCDIGEFKKYPLKEGYFNIFNMAKISSVEMLLNKSFLASNKIF
                     FDTKFGLGGIFDSGEEYIFCSDILKLKGTIKYYPIVLCQHPPISSGKNFYLTPEKIAA
                     KGAMFYRIFGNMGLLFSLAFSLKKHRIYKKKLGFFQFYRYMVSGQKHLKKLLTK"
     gene            6987..7733
                     /gene="wemE"
     CDS             6987..7733
                     /gene="wemE"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32281.1"
                     /translation="MNIEKKDYLVSIIMPCFNSEKYISEAIESVLKQTYSNFELIIID
                     DNSTDSTLKIINSFRDNRITIISFDENQGAGVARNKGIEVANGRFIAFLDSDDLWSKN
                     KLEAQIKFMLDNNYHLTYSYYQCFDKNGISNIKTPPLSISYNKLLYCNIIGCLTAIYD
                     SYIIGKQYMPLIRKRQDMGLWLKILKQYGDAYCLPYVLAPYRTDSGMTKNKFNAALYQ
                     WKFYKHELKFNLIKSLYYFIGYTYNGLFKK"
     gene            7760..8794
                     /gene="wzy"
     CDS             7760..8794
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ADL32282.1"
                     /translation="MFYYTLFLLIVLCLLILSNKIKNDFFFYILVIITVLFSGLRYDA
                     GNDFFSYYSMITNVVDINRLELIPRLLIEIAIAFEQPFLFFLLSSTCYIFSIAYFCKK
                     NSQNKELSFLLFILLPLSFLTSFGYIRQYMSIGFYLVALSLFLNKKKKKSFIFMLLAI
                     LSHTSSIIFIPLIILHKIISRKKINILITISLIIASYSFSWIIKEYSYLAGNYQHYIT
                     GKNVIDAGKKIGYICLLLLIYFYTLSIRIKNKNNIYFFNIFVIFVILYILLMDFGEYV
                     VRISYILFPISYILFSSTLLKNKNLRLLQLLAIILFGLSMFYTTLYLASKNTTRDFLT
                     NYSFIFLKNT"
     gene            8816..9982
                     /gene="ugd"
     CDS             8816..9982
                     /gene="ugd"
                     /note="UDP-Glc-6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="Ugd"
                     /protein_id="ADL32283.1"
                     /translation="MKITISGTGYVGLSNGILLSQNNKVVALDINKEKVELLNKKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYNLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIHNLDEFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     gene            10008..11012
                     /gene="gla"
     CDS             10008..11012
                     /gene="gla"
                     /note="nucleotide sugar epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gla"
                     /protein_id="ADL32284.1"
                     /translation="MKYLVTGAAGFIGFHLIKKLIQQGETVVGIDNLNDYYDVALKEA
                     RLNLLNQLDNFSFSFIDLADREKIAQLFEAEKFDRVIHLAAQAGVRYSLINPFSYADS
                     NLTGFLTILEGCRHNNVKHLVYASSSSVYGLNDELPFSPHDQANHPVSLYAATKKANE
                     LMAHSYSHLYGIPTTGLRFFTVYGPWGRPDMALFKFTKAIINNQPIDIYNHGEMKRDF
                     TYVEDIVEGVTRIADVIPTAQQDWKVSTGTPADSSAPYKVYNIGNGSPVNLMDYISAL
                     EIHLGKKADKNMLPMQPGDVYTTWADTEDLFKATGYKPQTSVDEGIKQFVDWYKIYYK
                     "
     gene            11035..11985
                     /gene="gne"
     CDS             11035..11985
                     /gene="gne"
                     /note="UDP-glucose 4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ADL32285.1"
                     /translation="MITIIGGSGFIGTRLSGQLTKENIEFKIVDIVKSEKYPEKWVFG
                     DVTRPESLLEPLKGSDVIINLAAQHKDNVHPISLYYEVNVDGAKNICDVAEQLNIKHI
                     VFTSSVAVYGFVEKETGEDGEFHPFNDYGKSKLEAEHKYETWYKKDNTNTLVVIRPTV
                     VFGEGNRGNVYNLFRQIASGKFLMVGSGNNKKSMAYVENIAAFLKFATQIESGHHIFN
                     YIDKPDFTMNELTDIIYTALDKRHSHIKVPYAIGLLGGYCFDILSKITRKEFPVSSIR
                     IKKFCARTQFKSNNIDKTRFKAPVTLEQGIANTVKAEFSH"
     gene            11989..12492
                     /gene="wemF"
     CDS             11989..12492
                     /gene="wemF"
                     /note="methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WemF"
                     /protein_id="ADL32286.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLESEGLSPDNAQSPTGARLFALTTKGTPAHSNVSYQ
                     DGDYLLFGPETRGLPPYVLDNMPTQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     gene            complement(12621..13598)
                     /gene="wemG"
     CDS             complement(12621..13598)
                     /gene="wemG"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ADL32287.1"
                     /translation="MKNNMPKITVLMSVYNDEKYLQESIDSILNQTFKEFEFLIINDG
                     STDNSLSILKENAKKDPRIKLIINEQNIGLIASLNKGLQLASCDLVARMDSDDIAVST
                     RLEAQYQFMLQHPEVSVCGGAFIYQEEPNRINTYPETHSDIVISSLYDCSLAHPTIIY
                     RKQDILSVGGYDKADLYAEDYGLWVNLIIAGKFKLHNLPVPLLYYRTHPNVERSQYKA
                     IQKDTAERVGRKLLMYFGINVNKEDYSLLLDNDKKTVEQVVRCKRLLEKINQQFNAKE
                     YRKELIQKNEFKKKLLERKVLVTDFSSFLIKISARWYFLIRKITKKAKK"
     gene            complement(13608..14165)
                     /gene="wemH"
     CDS             complement(13608..14165)
                     /gene="wemH"
                     /note="serine acetlyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WemH"
                     /protein_id="ADL32288.1"
                     /translation="MHAQAKVTLAELKDCLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGDHVYIGCNSSIIGGEINIGDNAIIGAH
                     ALVLKDVGEGCRYINKMNFEINKYC"
     gene            complement(14168..15001)
                     /gene="cysE"
     CDS             complement(14168..15001)
                     /gene="cysE"
                     /note="serine acetlyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="CysE"
                     /protein_id="ADL32289.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPMLASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     gene            complement(15101..16114)
                     /gene="gpsA"
     CDS             complement(15101..16114)
                     /gene="gpsA"
                     /note="glycerol-3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="GpsA"
                     /protein_id="ADL32290.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTKEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSL"
BASE COUNT     5651 a   2596 c   2526 g   5424 t
ORIGIN
        1 gagttattac gtgaaaccgt aaaagcgaat gagctttgga acgatatttt agataatgcg
       61 aaatttgagg ctgaacagag caataaaacc ctaacagtaa ctgccccgcc aggcccatgg
      121 acgatttatt gcaatcctta ctcattagcg agtgcctttg aaaatatcgt tcgcaatgcc
      181 ttgcgttatt cacattctcg tattgaagtg gcctttaccg aacaaaatca gggtatcact
      241 attattgttg atgatgatgg cccaggggtt agcccagaag atcgcgagca tattttccga
      301 cctttttatc gtacggacga agcacgtgat agagaatccg gcggtacagg gctgggactg
      361 gcaatagttg aaaccgcaat tagtcaacat cgaggccatg ttaaagctga cgatagccca
      421 ttaggggggt taagagtgga gatttggttg cctaggggaa gtattagctc aagcaataaa
      481 ttataattat catttttaac tattgataat tactgtatat tttaccctat taatttatac
      541 aaaatgtact aagttcttag ggtaaaatat gaaattttta gtcgtaggct gtgggttaac
      601 aggggctgta attagtcgcc atctagctga tgctggtcat tacatcacga taatagacca
      661 aagatcacat attgctagta attgccatac agaacgagat agaaataccg gtattatgga
      721 acatgtttat ggtccacata tcttccacac tagcgatgaa gtggtatggg aatatatcaa
      781 taaacatggt gaaatgatcc cttatgtaaa tagagtaaaa acaacttatc aaaatcaagt
      841 atattcttta cctattaacc tacatacaat aaatcaattc taccaaaaag cgctaaatcc
      901 tgaacaagca aaaacttttt taagtactat cacagataaa tctattactg atcctaaaaa
      961 ctttgaggaa caagccctaa aatttattgg aaaagattta tatgaagcct tttttaaagg
     1021 ctatacagta aaacaatggg gttgcgaccc taaaaatcta cctgcgagta ttttaaaacg
     1081 cctccctgta agatttactt atgatgacaa ttattattct cataaatacc aaggaatgcc
     1141 taaacatggc tataccgata tagttcgttc tatccttgat cataaaaata tacaagtaat
     1201 aacaaataaa aagtatgacc atagcgaaag agaagaatat gaccatatta tatggactgg
     1261 aaaaatagat gaatggtttg gctattgcga aggtagatta aactatcgaa cactagattt
     1321 tataaaacat gaaactatag gtgactatca aggtacagct gttatgaatt actgcgatat
     1381 agcggtacct tatactcgca tacatgaaca taaacacttc tcaccatggg aatctcatga
     1441 taaaacgatt tatattgaag aacatagtcg gatatgcgaa gagaatgata ttccttacta
     1501 tcccattcat ttagaagaag aaaataaaat attaaacaaa tacttagaat tggctcaaaa
     1561 agaaaaatat atcacttttg caggtaggct aggtacatat agatatatgg atatggatgt
     1621 atcaattaaa gaagcactta tgcttgcaga aaacttagta tcagatttaa tagatcaaaa
     1681 tgaacttaag tcgttctacc acaaacacca ataacaagag aatattctat gtcaaaattt
     1741 ttagcagtaa ttagatgtgg agataattca cttcataaaa actgggttaa tgataatgcc
     1801 caatttgatg taattcttag ttattttggt gaagatatcc cttattcttt agataatata
     1861 aaatatgtac accactttaa aggatcaaaa tgggaaggat tatacgattt attccataac
     1921 catccagagt tatgggctga ttatgattat atctggttgc ctgatgatga cttagacagt
     1981 accgtagaaa atatcaactt attttttgaa ttaatgcaaa agtatcaatt cgatctttgc
     2041 caaccagcat taactaataa tagttactat tcctataaag acttactaca agaaccggat
     2101 ttaatatata gagaaactaa ttttgtagaa gtaatggcgc cctgcttttc aaagaaaatt
     2161 atttctaaag catatcaaac atttaatgaa aataaatcag ggtggggact agatttttat
     2221 tggcctattt tatttaaaga agaaaatatt aaagttggtg ttatagactc tacaccaata
     2281 catcatacac gaccagtagg gatagcaggc catggaagta gtgataaaac cttatcgcct
     2341 ttaaatgaac ttcatcaatt attagacaag tattcacttg aaatacctac gtttgtagca
     2401 acttcacttc taacccaaaa agggaaaatg atacaaaaag gcagtttcag ctattcttta
     2461 agaaagttta tgaaaaaaag aaaataaccc tatgattaaa ttaagaaatt taccataaaa
     2521 tcacataaaa ctactttcat atcaaccata aggactgttt ttatattgaa taaatacagt
     2581 ctaattacct gatacaaagt aaatacattt tgagtaatgt agttttaacg cttacagcaa
     2641 tacaataaaa aaattactac attaaaatat tctgccattt attgaagagt aggtggcata
     2701 ttcttttcat tcccatcttt atagatattt cagaatgatc ttgctaaatc agtcgatatt
     2761 ccgatactta catataccta atcaatgtta gaatacccaa caattgcttg acccgcaggt
     2821 ttgaagagtc taatctcaaa gcggttttgc gtactgttaa agtatgctta tataataaaa
     2881 ctcttatatt acagatagat aagggtttta aaagagaaat tttggcgctc tgggggccgt
     2941 aaacccagtg cggtagctat ggattaaaat gatgaatgaa tatataaaac atatacgaaa
     3001 aaaagtctta agaagcttat gcaatagttt tcctacacta gttacaaaat tattatatta
     3061 cagacgattt ggcaaaagat taaatttaaa acaaccaaaa acatttaatg aaaaattaca
     3121 atggttaaag ttaaatacat ataaaaacaa tttattagtt acacaatgtg ctgataaata
     3181 taaagtaagg gaatacataa aaaaaacaca ttgtgaagat attttaaatg atatttatta
     3241 cacttggaat tctcctttag aaataaattg ggaagctcta ccaaataaat ttgtattaaa
     3301 atgtaatcat ggagcgggtt ataacattgt atgtagagat aaagaaaaat taaatcaaga
     3361 taagataata acaactttaa ccaaatggat gaaagaagat tattggaaga attctgtaga
     3421 actatgttac aaagatattc ctaaaaaaat tatttgtgaa aaatttatag aaacagaaag
     3481 taaagaatta ccactagatt ataaagtatt ttgttttcac ggtaaggcag aattcgttat
     3541 gatttgtact gatagagaaa gccaaaaacc aaaatttttt ttcgtagata aagattggaa
     3601 tttgttacca tacggcctag attataaata tataacagat gcctctatcc taacaaaacc
     3661 tattggttat gagaaattat ttttctatgc agagaagcta tcaaaaccat ttccttttgt
     3721 aagagcagat ttctatttaa atgataataa tattcttttt ggtgaattaa cctttacacc
     3781 cgccgccggt ttagatatag aattaaacaa caaagaaata agaaatgttg atataattat
     3841 aggaaatcta cttaatctga atagaattta aatatggaat taacaaaaaa aaaagaaatt
     3901 ttaagtaatt tggtaagcct tagctctatt aacatattag gaatattaat ccctataatt
     3961 acaatgccta ttctttctag aagcctagga attgatactt atggagaata tctgttattt
     4021 atgactatat taatattcgg tcatacaatt acagattata gtgttcaata tataggagtt
     4081 aggcaagcat ctaatcataa atataacaat atcaaacttt ctgttatata cattaattac
     4141 caaacattaa gattatttct aggtagtgtt tattttttat tatctcttag ctattctata
     4201 tgctttttaa acgtccattt cacgtactgg atattatatg gaggaagttt gtatctaata
     4261 ggctatgtgt tgactagcgc atggttttat ttatcaattg gaaacacaaa aatacttatt
     4321 atctcttctc tatttactaa attaataaat ttattaataa taatattttt cataaaaaaa
     4381 agtgacgata tagatttatt aattttatct actactttac ctctttttat ttctggtttc
     4441 ttattatatt taaatatcaa actaaaattc aaattaaaat ttatttttag agctaaaaat
     4501 atttatcgat atattattaa aggaaaaaat gtattcttag gtattctatc tcccaatttg
     4561 tataattcat tgcctataat aatactcggt tcaatatcga ctcctagtga attttcaaaa
     4621 tttgcaattg caaccagaat cattggatta atcacatcat ttcaagatat ctttgcacga
     4681 tcagtattcc ctatcctttc acgtgaaaag aaaaatcata taattactct actaaaaata
     4741 aattcactct tctcagtctt agcggttgtc tttattttat gttttggaga atactttcta
     4801 tatttatttt tagggaaaga ttatgctaat aatatttatt taaatatttt atctatcagc
     4861 attatattca ttggattcat gaattcattt tctgatggct tctttttacc taaaaagtat
     4921 gacttaattt atcgcaacat ttctattaga atatctttaa tatcctgtac tatatcattt
     4981 attttaatat taaaatttgg tattcttgga gggtgcatag gtataactac agcaagaatg
     5041 ttattctcta tagattattt tagaacatat tataatttat ctaaaaaggt ttagtatgaa
     5101 tatactaata ttattacctt cactaggtgg tggtggtgct gagaaaaatg cagttttaat
     5161 tgcaaatcaa ttagcaaaaa aaaacaacgt ccatatagta attatatata atgaaattac
     5221 taatgaaaac aggaagcggt tagataataa cgtatcgtta acattattaa ataagaaaaa
     5281 aataatattt tccatgtgga aaataaggaa tattatcctt aaagaaaatt atgatgtaat
     5341 tcttactact gtagcatatt ttggtataac attctctttt ttgcttccat ttataaatag
     5401 aaagaagaaa atatatatat atagggaaac taatatccca tccatatata ataaaaactt
     5461 taatattttg aaaagaatta tatttaagct tggctatctt tatacaatga agaattatga
     5521 ttatattatt tgccaatcaa atgatatgat aaatgactta aatatatatt catctattcc
     5581 ctcaaggaaa ataataaaaa ttaacaaccc aataactaga cctgaatgtc acccgtctat
     5641 aataaataac aaaaaatctg aaaattatgt ttttgcagca ggccgactca cccaacaaaa
     5701 aggattttta gatttaataa aaaactattc aacatctaat gctctaaaga aaaacataaa
     5761 attatatatt gcaggtatgg ggcctcaata taatgaaatg aaaatactaa ttaatagttt
     5821 aaatttgaat gcaaacataa ttttattagg gtttaaggat gatttaagta gttatttatt
     5881 aaatgcaaaa gcttttattc tttcatcaaa ttatgaaggt tttccaaata ttgtcttaga
     5941 agcattgtct tatgggtgcc ctgtactttc taatgattgc cctggtggta ttaatgaaat
     6001 tattattcct aatttcaatg gattaattta ttcgaaaaat aactttaatg aaatgttaga
     6061 tttaattctt aagattgatt ttgatagaaa tcaaatttct gaagatatca taaaaaggta
     6121 tgatgctaat ttattactaa gtaaatatga tgagataata gacaatgata gcaatatcaa
     6181 tttcaaccac aacaaataga ttattcaata taaaaaaaga gcaattccct caacaagaag
     6241 gtatcattta tttcatatct atacaaggac tagatatcga taaaaataaa gtaggtttat
     6301 ttataaaaaa caatctacct tattttagag tgttatatac tgaatctaaa ggtttatcta
     6361 ataatagaaa taattcatta aaagaattat acaagataaa tacggttgaa tacatatata
     6421 ttgcagatga cgatatctat gtaaatataa aaggaatttt tcaactagta gaaaaaatga
     6481 aattaaatga tatttcatta ggctgtggaa aaatatcttg cgatatcggt gaatttaaaa
     6541 aatacccctt aaaagaaggt tactttaata tttttaatat ggctaaaatc tccagcgtag
     6601 agatgttatt gaataaaagt tttttagcat ccaataagat tttttttgat acaaaatttg
     6661 gtttaggagg catttttgat tctggtgaag aatatatttt ttgctccgat attttaaaat
     6721 taaaaggtac aataaaatat tacccaatag tcctttgtca acatcctcca atctcatcag
     6781 gaaaaaattt ttatttgact cctgagaaaa ttgcagcaaa aggcgcaatg ttctatagaa
     6841 tatttggaaa catgggatta ttattttcac tagcgtttag cttaaaaaaa catagaatat
     6901 ataaaaaaaa gttaggcttt tttcagttct atcgatatat ggtttcaggt caaaaacatt
     6961 taaaaaaact attaacaaaa taaattatga atatagaaaa aaaagactat ctagtttcaa
     7021 taattatgcc ttgttttaac tctgagaaat atatttcaga agctattgaa tcagtattaa
     7081 aacaaacata ttcaaatttc gaattaataa taattgatga taactcaact gattcgacac
     7141 taaaaataat aaattcattt agagataaca gaatcactat tatttccttt gatgaaaacc
     7201 aaggtgctgg tgtagcaaga aacaaaggca ttgaggtagc aaatggtcga tttattgctt
     7261 ttctagatag tgatgattta tggagcaaaa ataaactaga agctcaaata aaatttatgt
     7321 tagataataa ttatcattta acatatagtt attatcagtg ctttgataaa aatggaatta
     7381 gtaatattaa aactccccct ttatctatat cttataataa acttttatat tgcaatatta
     7441 ttggatgtct aactgctatt tatgattcct atataatagg aaaacaatat atgcctctca
     7501 taagaaaaag acaagatatg gggttatggt taaagatttt aaaacaatac ggtgatgcat
     7561 attgtctccc ttatgtacta gcaccttata gaactgattc aggtatgaca aaaaataaat
     7621 ttaatgcagc attgtaccaa tggaagtttt ataagcatga attaaaattt aacttaataa
     7681 aatcattata ttattttatt ggttatacat ataatggatt atttaaaaaa taatttatct
     7741 ttttcacagg aataagattg tgttttatta tactctattt ttgcttatag ttttatgttt
     7801 attaattcta tctaataaaa ttaaaaatga ttttttcttt tatattttag ttattatcac
     7861 ggttttattc tctgggctca gatatgatgc tggaaatgat tttttttctt attattcaat
     7921 gataacaaat gttgttgata taaatagatt ggagttaata cctagacttt taattgaaat
     7981 agcaatagca tttgaacagc ctttcttatt ttttctttta tcatctactt gctatatttt
     8041 ttccattgct tatttttgta aaaaaaactc acaaaacaag gagttatcct ttctactatt
     8101 tatattatta ccattatctt ttttaacatc atttggttat attcgacaat atatgtctat
     8161 tggcttttat ttagtagctt tatctctatt tttaaataag aaaaagaaaa aatcatttat
     8221 ttttatgttg ctggctatac tttcacacac ttcatcaata atatttattc ctttaataat
     8281 attgcataag ataatatcca gaaaaaaaat aaatatttta attacaatat ccttaataat
     8341 agcatcatac tcattttcat ggataataaa agaatactct tatttggccg gaaattatca
     8401 gcattatata actgggaaaa atgtaataga tgccggaaaa aaaataggtt acatttgttt
     8461 acttctactt atatattttt atacattatc aattagaata aaaaacaaga ataacattta
     8521 cttttttaat atatttgtta tttttgttat actttatata ttattaatgg attttggaga
     8581 atatgtagtt agaattagtt acattttatt tcctatcagt tatatacttt tctcttcaac
     8641 cttactgaaa aataaaaatc ttagactatt acaattgcta gctataatat tgtttggact
     8701 atctatgttt tatacaactc tttatctagc tagtaaaaat acaactcgtg actttttaac
     8761 aaattactct tttatttttt tgaaaaatac ttaattttta tttaggattt agaatatgaa
     8821 aatcaccatc tcaggaaccg gctatgtcgg tctatctaat gggatactcc tttcccaaaa
     8881 caacaaagtt gttgcattag atattaataa agaaaaagtc gaactattaa acaaaaaaat
     8941 ctctcctatc tctgataaag aaattgaaga gtttttagct actaaagagc tcgactttac
     9001 ggcaacttta gataaagaat tcgcttataa agatgctgaa tttgttatca ttgccactcc
     9061 taccgattat gatcctaaaa ctaactactt taacacctct tctgtagagt cggttattcg
     9121 tgatgtttta gaatataatc caaacaccac tatgattata aaatcaacca ttccagttgg
     9181 ttttactaaa cagatgagag aaaaatatca taccgataat attatcttct ctcctgagtt
     9241 cttacgtgaa ggtcgcgccc tttacgataa cctttatcca tctcgtattg tgattggtga
     9301 gcaatctgca agagcagaga aatttgctga gcttttacaa caaggggcat taaaaaaaga
     9361 tattgatgtt ctttttactg attctacaga agcagaagcc attaagttat ttgccaatac
     9421 ttatctcgca ctgcgtgtgg cttattttaa tgaactcgat agctacgccc aagcttacaa
     9481 tttaaatgca agacaaatta ttgaaggtgt ctgtttagat ccacgtattg gtaatcacta
     9541 taacaatcca tcatttggtt atggcggtta ctgcttacca aaagatacga aacagctgtt
     9601 agccaactac gaatcagtgc ctaataatat tattggcgcc attgtggaag ctaaccgcac
     9661 tcgtaaagac tttattgctg attctattat cgctaaatca ccgaagattg ttggtgttta
     9721 tcgtttaatt atgaaatcag gctctgataa tttccgctca tcttcaattc aaggcattat
     9781 gaagcgtatt aaagcaaaag gcattgaagt agtgatttat gagccagaaa tgaaagaaga
     9841 gactttcttt aactcaaaat taattcataa tttagatgag tttaaagcaa tgtctgatgt
     9901 cattattacc aatagaatgg cttcagcact aaacgatgtt gaagaaaaag tttatactcg
     9961 agacctcttt ggtaacgatt aattaataga aacggattaa ttacgtaatg aaatatttag
    10021 tcacaggtgc tgctggattt attggttttc atctaataaa aaaactgatc cagcaaggtg
    10081 aaaccgttgt tggtattgat aaccttaatg attattatga tgttgcttta aaagaagcta
    10141 gacttaatct tttaaatcaa ctagataatt tctctttttc ttttattgat ttagctgata
    10201 gagaaaaaat tgctcaatta tttgaggctg agaaatttga tagggttatt catttagctg
    10261 cacaagctgg tgttagatat tcactaatta atccgttttc ttatgctgat agtaatttaa
    10321 ctggttttct aactatttta gaaggttgcc gacacaataa cgttaagcat cttgtttatg
    10381 catcatcaag ttcagtctat ggcttaaatg atgaattgcc attttctcct catgatcagg
    10441 ctaatcatcc tgtttcttta tatgcagcca ctaaaaaagc taatgaatta atggcgcata
    10501 gctattctca tttatatggt attccaacta ctggattacg ttttttcaca gtctatggcc
    10561 cttggggacg ccccgatatg gcattattta aatttactaa agccattatt aacaatcagc
    10621 ctatagatat ttataaccat ggtgaaatga aacgtgattt cacttatgtt gaagatattg
    10681 tagaaggtgt tacacgtatt gctgatgtga tccctacagc ccagcaagat tggaaagtaa
    10741 gtacaggtac tccagcagat agttctgcac cctataaggt ttataatatt ggtaatggat
    10801 ctccggtaaa tttaatggat tacattagtg cgttagaaat acatttaggt aaaaaagcag
    10861 ataaaaatat gttaccaatg caacccggtg atgtgtacac cacatgggca gatactgagg
    10921 atttatttaa agcaactggc tataaacctc aaacaagcgt tgatgaaggt ataaaacagt
    10981 ttgttgattg gtataaaatt tattacaaat aataattaac ttaatggatc tattatgata
    11041 acaatcatcg gtggctcagg ctttattggt actcgcctat caggccaatt aactaaagaa
    11101 aatattgaat ttaaaattgt ggatattgtt aaaagcgaga aatatcctga aaaatgggtc
    11161 tttggtgatg ttactagacc agaatcttta ctcgaaccat taaaaggttc tgatgtaatt
    11221 atcaatttag cagcgcaaca taaagataat gttcatccta ttagcttata ttatgaagtt
    11281 aatgtcgatg gtgctaaaaa tatttgtgat gttgctgaac aattaaatat taaacatatt
    11341 gtatttactt catctgttgc tgtatatggt tttgtcgaaa aagaaacagg tgaagatggg
    11401 gagttccatc catttaacga ttatggaaaa tcaaaacttg aagctgaaca taagtatgaa
    11461 acttggtata aaaaagataa tacaaataca ttagttgtaa tcagaccaac cgttgtattt
    11521 ggtgaaggca atagaggaaa tgtatacaac ctattccgcc aaatagcttc aggtaaattt
    11581 ttaatggtag gctcgggtaa taataagaaa tctatggctt atgtagaaaa tattgcagct
    11641 ttcttaaaat ttgctactca aatagaatcc ggtcatcata tatttaatta tatagataag
    11701 cctgatttta ctatgaatga gttaaccgat attatctata ctgcactcga caaacgccat
    11761 agtcatatta aagtacctta tgctattgga ttacttggtg ggtattgttt tgatatttta
    11821 tcgaaaataa caaggaaaga atttcctgtt agtagcattc gtattaaaaa gttttgcgcg
    11881 cgtacccaat ttaaatctaa taatatagat aaaaccagat ttaaagcacc tgttactctg
    11941 gaacaaggca tcgcaaatac agttaaagca gaatttagtc attaaacaat gctcaatatc
    12001 gtcttatttg aacccgaaat tccaccgaat acgggtaata ttatccgcct gtgtgccaac
    12061 acaggctttc atcttcattt gatccaacca ttgggtttta cttgggatga taaacggcta
    12121 cgccgtgcag ggcttgatta tcgtgagttt gctgatatca aacagcatca cgattattat
    12181 gcttttttag aaagtgaagg attaagccct gataatgcac aatccccgac aggtgcacgg
    12241 ttgtttgcat taaccactaa aggtactccc gcccatagta atgtaagtta tcaagatggg
    12301 gattatctgc tatttggtcc tgaaacacga ggattaccac cttatgtgtt ggataatatg
    12361 ccaacacaac aaaaaatccg tgtaccaatg ttagcggata gccgcagtat gaatctttct
    12421 aatacggttg ccgtggttgt ttttgaggca tggcgccaat taggctatcc tggtgcatta
    12481 ttaagagatt aatttatctt tttaatttat aaccataaaa aatgacctct agactttatt
    12541 gtctcgaggt catttttatg tatatgcttt gtgctagttt tagcttttct tattttatat
    12601 cttcttttta tctttcggtt ttatttctta gcttttttag ttatttttct tatcaagaaa
    12661 taccatcttg cacttatttt tattaaaaat gatgagaaat cagttactaa tacctttctt
    12721 tctaataatt ttttcttaaa ttcatttttt tgtattaact cttttcgata ctctttagca
    12781 ttaaattgtt gattaatctt ttcaagtaac cttttacagc gtactacttg ctctactgtt
    12841 tttttatcat tatccaataa taatgaataa tcctctttat tcacgttaat gccaaaatac
    12901 attaataatt ttcgtcctac tctttccgcg gtatcttttt gtatcgcttt atattgcgaa
    12961 cgttcgacat ttggatgagt acgataataa agaagtggta cagggagatt atggagctta
    13021 aattttccag caataattaa attcacccat aaaccataat cttctgcata gaggtctgct
    13081 ttatcatatc caccaacaga taatatatct tgcttacgat agataatggt tggatgcgct
    13141 aaggagcaat cataaagact agaaatcaca atatcactat gtgtttctgg ataagtatta
    13201 attctatttg gctcttcttg gtagataaaa gcaccaccac aaacactcac ttcaggatgt
    13261 tgtaacataa attgatattg cgcttctagt ctggttgaaa cggcaatatc atcagagtcc
    13321 attctagcga ctaaatcaca tgaagctaat tgtaatcctt tgtttaaaga agcgatcaat
    13381 ccgatatttt gttcattaat aatcagtttt attctgggat cttttttggc attttctttt
    13441 aaaatagata atgagttatc cgttgagcca tcattaatga taagaaactc aaattcttta
    13501 aatgtttgat ttaatataga atcaatagac tcctgaaggt atttctcatc attgtatacc
    13561 gacattaata cggtaatttt gggcatattg tttttcatta tctattttca gcaatattta
    13621 ttaatttcaa aattcatctt attgatatac cgacaccctt cacccacatc ctttaatact
    13681 aatgcatggg cgccaataat cgcattatca ccaatattaa tctcaccacc aataatggat
    13741 gaattacagc caatataaac atgatctcct acttggatat tggcttttcc ccctttctct
    13801 tttacgccaa tagtcacccc ttgcataata gtgacatatt cccccagttg gcagtaatta
    13861 gtcagtacaa tgccaatcgg gtgtggtaaa gaaaggcctt taccaatacg agtttttaac
    13921 gagatatcat tacagtattt acggttattg ctagcaccga tccgaatacc taatttatgt
    13981 aaaaagcgat ggggagagaa ataaaaatat tgtgctaccc gccaccaaaa taggtaacgg
    14041 gaacgctgac agaaaaatac tcgatgaaat agccttaacc aactaaaggg ttttttatca
    14101 ccaatatatt cagcctgtaa gcaatctttt agttcagcaa gggtaacctt agcttgggca
    14161 tgcatagtta aataccatca ccacactcaa agccgggtac aacaccatta aagtgttgat
    14221 ccatatcgaa tgacggtttt tcactatcag gacgaccgac aatgcgggca ggcaccccgg
    14281 ccaccgtagt atgagctggt acttcatgta atactaccga gccagcacca attttagcac
    14341 cacgaccaat ttcgatatta ccaaggattt tagctcctgc gccaatcatt accccttcac
    14401 gcactttcgg gtgacgatca cctcctgttt tacctgttcc ccctaaggtc acagattgta
    14461 aaatagagac atcattttca accaccgccg tttcaccaat cacaatacct gtcgcatggt
    14521 cgagcataat accgtgtcca atacgtgccg ctgggtggat atccacccca aaggtgactg
    14581 atatttggtt ttgtagataa atagctagcg cttggcgatt ttgttgccat agccaatggg
    14641 caatacggta ggcttgtagt gcatgaaacc cttttaaata gagcagtggg gtagaatatt
    14701 tatccaccgc gggatcacgt aggcgtaccg cggagagatc acaagcggcc gactcaatca
    14761 ttgagctatc gtgactataa gcctcttcaa caatttcgcg aactgcaatg gctggcatag
    14821 tctgagttgc cagcttattg gccagcatat agcttaatgc actacctaaa ttttcgtgtt
    14881 tgagaagtgt cgcatgaaag aaactggcta acattggctc acaatctgcc aatgcacgcg
    14941 cttcgctttt aatatgatcc cagactcttg ttagttcttc tagcgacata ctcactctca
    15001 ttttttacac caaagcctaa gtaaatactt aggctttgaa ttttcaaaca aatggatagt
    15061 taaaacagat accctaagaa tattatttaa aataaactga ctacaggcta ctgtctatct
    15121 catctttggt tgctcgccct aataaagcca atgcagcctc ttttacatct ttatgttgat
    15181 ataaaatttg gtaaatctgt tcggtgattg gcatttctac acccacttgt tcggctaatg
    15241 cgcgaacttc tttggtgtta cgataacctt cgactacctg gccaattttc tcttgggctg
    15301 tatcaacatc taaaccttga cctagcatca taccaaagcg acgattacgt gattgattgt
    15361 ctgtacaggt taaaaccaaa tcacccaaac cagccatgcc cataaaagtt gccgcatctg
    15421 cacctaaggc tttacctaag cggctcattt cggctagacc tcgggtaatc agtgcggtac
    15481 gcgcattagc gccaaatccc ataccatcag acatacccgc accaatagca atcacgtttt
    15541 taacagcgcc acccagttgc acaccgataa aatcagggtt tttatagact cggaaacttt
    15601 taccacaatg aaatagctgt tgtaactgct ctaaaaagag attatccgtc gaggccactg
    15661 caatcgcggt gggcaaaccc gcggcaagct ctttagcaaa agtagggcca gataatactg
    15721 cgagcgggat ttcattacct aatacttcac gggcaacatc ttgtaataag cgaccagtat
    15781 gtgcttcaag accttttgtc gcccaaacaa cacgtgcatc ctgacgtaaa aagggtttga
    15841 tttgttgcag tacttcacca aacacatggc ttgggatcac cacaagaata ttacggctcg
    15901 cttcaatcgc tttttgcaaa gaagcttcca tatataaact atcaggaaag gaaacatcgg
    15961 gcagaaaggc ttgattacag cgcgcttgtt ctaatgccgc aacgtgcttg ggatcatgcc
    16021 cccacagcac aacatcgtgc ccattacgcg ctaaggtaat cgctaaagcg gtgccgtatg
    16081 aaccggcacc gataactgtc atagaagcgt tcattaagct tcttcaactc ctgagtttgc
    16141 tgcatcctgt tgctgttgtt gctgtaaata gttcataaac aacgcatcaa agttaac
//
