LOCUS       KY710712               16014 bp    DNA     linear   BCT 19-SEP-2018
DEFINITION  Proteus mirabilis strain PrK 66/57 O antigen gene cluster, complete
            sequence.
ACCESSION   KY710712
VERSION     KY710712.1
KEYWORDS    .
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 16014)
  AUTHORS   Yu,X., Torzewska,A., Zhang,X., Yin,Z., Drzewiecka,D., Cao,H.,
            Liu,B., Knirel,Y.A., Rozalski,A. and Wang,L.
  TITLE     Genetic diversity of the O antigens of Proteus species and the
            development of a suspension array for molecular serotyping
  JOURNAL   PLoS ONE 12 (8), e0183267 (2017)
   PUBMED   28817637
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16014)
  AUTHORS   Yu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2017) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin
            300457, P.R China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16014
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PrK 66/57"
                     /serotype="O40"
                     /db_xref="taxon:584"
                     /collected_by="Czech National Collection of Type Cultures,
                     Prague, Czech Republic"
     misc_feature    1..16014
                     /note="O antigen gene cluster"
     gene            8..1258
                     /gene="cpxA"
                     /note="coding region not determined"
     CDS             1639..3183
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="AXY99723.1"
                     /translation="MNMTYNIKNKIIIKNTLFLYFRMILTLVITLYSSRIVLKTLGVE
                     DFGIYNIVGGVVTMLTFISGAMTSATQRFLSFEIGKDNKEKLSKTFIMSINIHIMIII
                     CIFIIAETAGLWLINNYLIIGPDRIKAANIVFQYSLLSFCFTILSTPYKANIIAHEKM
                     NIFAYIGIIDVFLKLIAVLMLEYILGDKLIIYSILISIISISIYLILLAYNRLHFDTT
                     KYKFYWNRKLFFSLFSYTGWNLFGNMASVGMNQGINILLNIFFGPIVNAARAISFQVN
                     NAINGFVINLQSALSPQIVKSYAKNDISYLENLVLLGSKYSFFLILILSLPILLKPDI
                     VLSLWLGNYPAYTSIFCQLILIDSLINSLSSSLMTAFQASGKIKKYQITIGSILLLNI
                     PISYFLLSYGFEATITFAVSIVLSLISLIVRLLLLNELFPGISDKFYKTIFWRVIAIF
                     SISYTLSWFITKDLKNDLFNFILTCITSWSFLIINIIFLGLTKNERLKVKNILVKKYI
                     KFKENK"
     CDS             3183..4547
                     /note="orf03"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf03"
                     /product="4Fe-4S ferredoxin"
                     /protein_id="AXY99724.1"
                     /translation="MKNNQCCNVIKLVVNNNLCIGCGLCVSKCKNNSLIMSENKYGFY
                     EARQIKDCSCDGECLSVCPFNPYPNEKVKTENEISEYFFDNQKKHHPKIGYYISTYAG
                     YSHKHRKSSSSGGIATYVLEKLLDNKTINHVISVKASQNGSTHYEYIISSSKEELFKS
                     AKTKYYPVTLSSVLKEINELDGSIAIVGVACFVKAIRLAQLNDPILKDKIKFIVGIIC
                     GGIKSSFFAEYLASKAGANPYEYKNPEFRIKDINSKASDYSFGCYDKKNNINLIKMSA
                     VGDMWGTGLFKSNACDLCDDVTTELADISVGDAWMKPFSDDGKGNNVIITRSELANDI
                     ITYGIKNKELNITKLSTDEFLKSQQGSFTHRHNALPFRIRRFKKLGILIPPKRIFNSR
                     IDILERLIQRMRMKTRRKSLELWLKEKNHITFDKKMKLDLTILKILTKLNHLKRRLII
                     KMKG"
     CDS             4551..5669
                     /note="orf04"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf04"
                     /product="pyruvyl transferase"
                     /protein_id="AXY99725.1"
                     /translation="MKIGILTQPLVTNYGGLLQAYALQKYLIKNDFEVLTVDIRPKKP
                     KLWGIKSIIVNIIKKYLLRRKNITILPISKKEKKTIRKYTSKFINENIKTTEKIKSIK
                     EINKLNKYNFDAYIVGSDQVWRPAYSPNITTYFLDFLKNDIKTIRIAYAASFGLDNCN
                     EFTQKQLKICSELAKKFHLISVREDSGVELCKKYFNLNAQHVLDPTMLLNKEDYIELI
                     KKDNISRNTGNMMTYILDSSPDKEKIINIVAKKYKLTPYSVMPDKNNKVYPPVTQWLR
                     GFWDAEYVVTDSFHGVVFSIIFNKQFIAIGNINRGLARFSSILKKFSLENRIIFSSKD
                     LNDEFFNHKIDYCLINEILEKEKNIASLTLKKALNTNK"
     CDS             5682..6587
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt1"
                     /product="glycosyltransferase"
                     /protein_id="AXY99726.1"
                     /translation="MISIIIPCYNDWQSLEKNLDSLLLFAKKIDGEVIIINDGSSDNI
                     ARDIKNIINPQLTYLEQENKGVSSARNKGILFAKKEKLLFLDSDDYINFDELIKHSEE
                     IKNFDLIYWDTIKVFPNNKNIIYKTYLEKDVNDLVYALLCRKYYLFMGSFIISKKISQ
                     KIMFDENYKYGEDLKFIYECIDSINSFTKLDNINLFYIQRCNSAMYTFSHRRFDSVKA
                     LESISIRNNTLKKIDLENFIKKDKKIILNNFIRSFPLGKIYSKRKEFIEINNIIKNIF
                     GKNKFIYELNFFLYLIIYKFYLLKK"
     CDS             6589..7656
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AXY99727.1"
                     /translation="MAIYIIFIITNLILLSIPFRSNNNIYYPLSFISYILILSFSGFR
                     YGIGTDYFNYESIFYNIHYDNSYNSGLEYGFYYLSKLLPQEKEGFFSLIFITSLITNF
                     TLFLAIKNRIKNKSPEERFLIILFFFISNLYFLPFNGIRQGIAFSCTLLSFTFIKQRR
                     FLYFLILILIGSLFHKSILLFIPFYFVYKINIKNLYCFIIVSISFILVKSGIFSLTIN
                     SLINILGEKYARYLENPVDYGGSGLGVYLYVILFLFIYFIKFVVRNEKMKTKEYNFLF
                     LMFSIGISFRILALDNIIFVRPSYYFTVFDTFFIPFFIMCFNKNKFIISIVITCFYLI
                     SFLASLSNSNGLLPYQNIFIS"
     CDS             7664..8803
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt2"
                     /product="glycosyltransferase"
                     /protein_id="AXY99728.1"
                     /translation="MKKIAILVPNLKDNGVSRVAALHSIMFSNQGYLVDIIVEENKSV
                     KFPFSGNLINLNLKRKRGVFKILNFIKLYLKIKKIKTSNKYDFFISHIPHCDIINSIT
                     KTKNKEITISTVHNNVELRYSKITKLLLKIIILKKSDAVVTVSEDLASQLKKKYSNYN
                     KIINIPNPVDFNLIKKSLNAQLPEYLSNKKFILNIGRLDSQKGHWHLIKAFYLTLKKQ
                     PDLYLVIIGEGPEKIILKNLCKSLNIDKKVIFEGFKENPFIYMNKSSAFVFPSLYEGL
                     PMVLIEAMVCRTPIISSDCFSGPRELLIKDNITYGKLIPNYGTTSSLYTKSIGDYDRL
                     ISNEILLLLNCVANKKNLDLAENRAKEFNLENIFLRWKDLFDEYI"
     CDS             8790..9995
                     /note="orf08"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf08"
                     /product="poly(glycerophosphate)
                     glycerophosphotransferase"
                     /protein_id="AXY99729.1"
                     /translation="MSTSKNFRNKYLFHRILHFIKGILFSIYYSYIYTKRKKTIIFNS
                     ACNQEFTWNAKSLFLHSRNKLEALGFKVYFVINDKNKREYLTSLYGNYFICNKKKTNV
                     IKIYTAAIWILSTLETPCSGIFLNRKRFVYHLGHGTPIKNIGLCENNLGFLKKSFYRL
                     NKSNISLFLSSSHFFKKYMARAFGVNEEKIVVAPQPRIEDLINKNKNINFSFQKDKKY
                     ILYAPTWRHYDDVKLFPYIKDNIDNLNRELQQLNIIILLRLHPRFESNLNSYLRTNIL
                     NFNSKICEDISDALYFTSGLITDYSSIYCDYLLLKKPVALLPYDLDKYVKEIGFSFLY
                     KEIFPKTIIKNHTDFIDFCAQVSNDCFNIEEQNTLAEKLNFIPDNCSITDYNISIIMK
                     EYKKRWPSE"
     CDS             9980..10375
                     /note="orf09"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf09"
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="AXY99730.1"
                     /translation="MAVRIITFGTFDVFHIGHIKILERARSLGDYLIVGVSSDKLNFK
                     KKNRYPIYNQNDRIDIISSLKYVDEVFIEESLELKEYYIKSFNADILVMGNDWEGKFD
                     HLGKICKVIYLPRTPSISTTEIIEIAKAR"
     CDS             10442..11608
                     /note="UDP-glucose 6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="ugd"
                     /product="ugd"
                     /protein_id="AXY99731.1"
                     /translation="MKITISGTGYVGLSNGILLSQHNKVVALDINKEKVELLNQKISP
                     ISDKEIEEFLATKELDFTATLDKEFAYKDAEFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVLEYNPNTTMIIKSTIPVGFTKQMREKYHTDNIIFSPEFLREGRALYDNLYPSRIVI
                     GEQSARAEKFAELLQQGALKKDIDVLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     QAYGLNARQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIGAI
                     VEANRTRKDFIADSIIAKSPKIVGVYRLIMKSGSDNFRSSSIQGIMKRIKAKGIEVVI
                     YEPEMKEETFFNSKLIHNLDEFKAMSDVIITNRMASALNDVEEKVYTRDLFGND"
     CDS             11620..12123
                     /note="Methyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemW"
                     /product="wemW"
                     /protein_id="AXY99732.1"
                     /translation="MLNIVLFEPEIPPNTGNIIRLCANTGFHLHLIQPLGFTWDDKRL
                     RRAGLDYREFADIKQHHDYYAFLASEGLSTDNAQSPTGARLFALTTKGTPAHSNVSYH
                     DGDYLLFGPETRGLPPYVLDNMPIQQKIRVPMLADSRSMNLSNTVAVVVFEAWRQLGY
                     PGALLRD"
     CDS             complement(12263..13240)
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gt3"
                     /product="glycosyltransferase"
                     /protein_id="AXY99733.1"
                     /translation="MKNNMPKITVLMSVYNDEKYLQESIDSILNQTFKEFEFLIINDG
                     STDNSLSILKENAKKDPRIKLIINEQNIGLIASLNKGLQLASCDLVARMDSDDIAVST
                     RLEAQYQFMLQHPEVSVCGGAFIYQEEPNRINTYPETHSDIVISSLYDCSLAHPTIIY
                     RKQDILSVGGYDKADLYAEDYGLWVNLIIAGKFKLHNLPVPLLYYRTHPNVDRSQYKA
                     IQKDTAERVGQKLLMYFGINVNKEDYSLLLDNDKKTVEQVVRCKRLLEKINQQFNAKE
                     YRKELIQKNEFKKRLLERKILVTDFSSFLIKVSARWYFLIRKITKKAKK"
     CDS             complement(13250..13807)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="wemH"
                     /product="wemH"
                     /protein_id="AXY99734.1"
                     /translation="MHAQAKVTLAELKDCLQAEYIGDKKPFSWLRLFHRVFFCQRSRY
                     LFWWRVAQYFYFSPHRFLHKLGIRIGASNNRKYCNDISLKTRIGKGLSLPHPIGIVLT
                     NYCQLGEYVTIMQGVTIGVKEKGGKANIQVGNHVYIGCNSSIIGGEIKIGDNAIIGAH
                     ALVLKDVGEGCRYINKMNFEINKYC"
     CDS             complement(13810..14643)
                     /note="Serine acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /gene="cysE"
                     /product="cysE"
                     /protein_id="AXY99735.1"
                     /translation="MRVSMSLEELTRVWDHIKSEARALADCEPMLASFFHATLLKHEN
                     LGSALSYMLANKLATQTMPAIAVREIVEEAYSHDSSMIESAACDLSAVRLRDPAVDKY
                     STPLLYLKGFHALQAYRIAHWLWQQNRQALAIYLQNQISVTFGVDIHPAARIGHGIML
                     DHATGIVIGETAVVENDVSILQSVTLGGTGKTGGDRHPKVREGVMIGAGAKILGNIEI
                     GRGAKIGAGSVVLHEVPAHTTVAGVPARIVGRPDSEKPSFDMDQHFNGVVPGFECGDG
                     I"
     CDS             complement(14740..15756)
                     /note="Glycerol 3-phosphate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /gene="gpsA"
                     /product="gpsA"
                     /protein_id="AXY99736.1"
                     /translation="MNASMTVIGAGSYGTALAITLARNGHDVVLWGHDPKHVAALEQA
                     RCNQAFLPDVSFPDSLYMEASLQKAIEASRNILVVIPSHVFGEVLQQIKPFLRQDARV
                     VWATKGLEAHTGRLLQDVAREVLGNEIPLAVLSGPTFAKELAAGLPTAIAVASTDNLF
                     LEQLQQLFHCGKSFRVYKNPDFIGVQLGGAVKNVIAIGAGMSDGMGFGANARTALITR
                     GLAEMSRLGKALGADAATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGLDVDTAQEK
                     IGQVVEGYRNTEEVRALAEQVGVEMPITEQIYQILYQHKDVKEAALALLGRATKDEID
                     SSLL"
     gene            complement(15756..16013)
                     /gene="secB"
                     /note="coding region not determined"
BASE COUNT     5625 a   2488 c   2487 g   5414 t
ORIGIN
        1 aggtgttatg ctagagcaac atatagaagc cgaactagcg caagatccgg ctaacgatct
       61 gctatggtgg cggcgattaa tccgcgccat tgacaaatgg gcgccacctg gtcaacgtct
      121 tattattgtc accagtgaag gtcgtattat tggtgctcaa cgtaatgaga tacaagttgt
      181 cagaaacttt atggggcagt ctgataacgc agaccatccg aaaaagaaaa aatacggtcg
      241 ctctgagatg ttaggcccct tttccattag agatggtgaa gatcactacc aactttactt
      301 agtgcgccca tcaagtagcc cgcaatctga ttttatcaat ttattatttg ataaaccgct
      361 tttattactg atattcacta tgcttatcag tacaccgcta ctggtttggc tctcttggag
      421 cttggcaaaa ccggcccgaa aactcaaaaa tgcggcggat gatgtggcaa aaggtaatct
      481 gcgccctcac cctgaactgg aaaccggccc gcaagaattt ttagcggcag gcaccagttt
      541 taatcagatg atcagcgcct tagaacgcat ggttgaagcg caacagcgat tgatttctga
      601 tatctcccat gagttgcgca ccccattaac tcgtttacag cttgccagtg cattactacg
      661 tcgtcgtagc ggtgaaagta aagagctaga gcgtattgaa acagaaacac agcggctaga
      721 tggcatgatc aatgacttat tagtgctttc ccgcaatcaa tataaaaatg agttattacg
      781 cgaaaccgta aaagcgaatg agctttggaa cgatatttta gataatgcga aatttgaggc
      841 tgaacagagc aataaaactc taacagtaac cgccccgcca ggcccatgga cgatttattg
      901 caatccttat tcattagcga gtgcctttga aaatatagtt cgcaatgcct tgcgttattc
      961 acattctcgt attgaagtgg cttttaccga acaaaatcag ggtatcacta ttattgttga
     1021 tgatgatggc ccaggggtta gcccagaaga tcgcgagcat attttccgac ctttttatcg
     1081 tacggacgaa gcacgtgata gagaatccgg cggtacagga ctaggactgg caattgttga
     1141 aaccgcaatt agtcaacatc gaggccatgt aaaagctgac gatagcccat taggggggtt
     1201 aagagtggag atttggttgc cgagagggag tgtggtaaat aaatcacaac acgcttaatt
     1261 cagtaataaa attactacat taaaatattc tgccatctat tgaagaatag gtggcatatt
     1321 cttttcattc ccatctttat agatatttca gaatgatctt gctaattcag tcgatattcc
     1381 gatatttata tatacctaat caatgttaga atacccatca attacttgac ccgcgggttt
     1441 gaagagtcta atctcagagc ggttttgcgt actgttaaag tatgcttata taataaaact
     1501 cttatattac agatagataa gggttttgaa agagaaattt tggcgctctg ggggccgtaa
     1561 acccagtgcg gtagctttat ttttacttaa ataaatatct aaaaaaaaag ataaatatat
     1621 taaaacaaat attttataat gaatatgaca tataatataa aaaataaaat aataataaaa
     1681 aacacattgt ttttatattt tagaatgatc ttaaccttag taataacatt gtattcttct
     1741 agaatagtat taaaaacatt aggtgtagaa gattttggta tatacaatat cgttggtggt
     1801 gttgttacta tgttaacttt tattagtggg gcaatgactt cagcaacaca aagattttta
     1861 tcttttgaaa taggaaaaga taataaagaa aaattatcaa aaacatttat aatgagtata
     1921 aatatacata ttatgattat aatatgtata tttataattg ctgaaacagc tggtctatgg
     1981 ttaatcaata attatttaat aattggtcca gatagaatca aagctgctaa tattgtcttt
     2041 caatattcat tattatcatt ttgttttaca atattaagta cgccatataa agccaatatt
     2101 attgcccatg agaaaatgaa tatttttgct tatataggta taatcgatgt ttttctaaaa
     2161 ttaatagcag tgttaatgct tgaatatatt ttaggtgata aactcattat ttattctata
     2221 ttaatttcca tcatcagtat ctcaatttac ttaatattat tagcttataa taggctccat
     2281 tttgatacta caaaatataa attttattgg aatagaaaac tttttttctc actctttagt
     2341 tatactggtt ggaatttgtt tggcaacatg gcttctgtag gaatgaacca gggtattaat
     2401 atattattaa atatcttttt cggacctata gttaatgccg ctagagcaat atcatttcaa
     2461 gtcaacaatg caattaatgg atttgtaatt aatctacaat cagctttaag tccacaaatt
     2521 gtaaaatctt atgcaaaaaa tgatatttct tatctagaaa atttagtttt acttggctcc
     2581 aaatattcat tttttcttat tttaatccta tcactcccta ttttattaaa accagatata
     2641 gttttaagtc tttggttagg taactatcct gcatatacat caatattttg tcagttaatt
     2701 ttaattgatt cattaattaa tagcttgtct agttctttaa tgactgcatt tcaagcatca
     2761 ggtaaaataa aaaaatatca aattactatt ggctcaatct tactattaaa cattcctatt
     2821 tcgtattttt tactaagcta tggatttgaa gcgacaataa cattcgctgt tagtattgtt
     2881 ttatcattga tatctttaat agtacgttta ttgttattaa atgaattatt tcctggtatt
     2941 tctgataaat tttataagac aattttttgg agagtaattg cgatattttc tatttcttat
     3001 actttatctt ggtttattac aaaagatctt aaaaatgatt tgtttaattt tattttaact
     3061 tgtattacta gttggtcttt cttaattatt aatattatat ttttaggatt aacaaaaaat
     3121 gaaaggttaa aagtaaaaaa tattttagtc aaaaaatata taaaatttaa agaaaataaa
     3181 tgatgaaaaa taatcaatgt tgtaatgtta ttaaattagt agtcaataat aatttatgta
     3241 taggttgtgg attatgcgta tcaaaatgta aaaataactc tttaattatg tcagaaaata
     3301 aatatggatt ttatgaagca aggcaaataa aagactgtag ttgcgatggt gaatgtttat
     3361 ctgtgtgtcc attcaatcca tatccgaatg aaaaagtaaa aacagagaac gaaatatcag
     3421 aatatttttt cgataatcaa aagaaacatc atcccaaaat tggctattat atttcaacat
     3481 atgcaggtta ttctcataaa catagaaaga gctcatcttc aggaggtata gcaacatatg
     3541 tattagaaaa acttttagat aataaaacga tcaatcatgt tatatccgtt aaagcatccc
     3601 aaaatgggag cactcattat gaatatataa taagtagttc taaagaagag ctcttcaaat
     3661 ctgcaaaaac aaaatattat ccagtgacat tatcttcagt tctaaaagaa ataaatgaac
     3721 tagatggaag tatagctatt gttggtgttg cttgttttgt aaaagcaatt agattagcac
     3781 aattaaatga tccgatactg aaagataaaa taaaattcat tgttggtatt atttgtggtg
     3841 gtataaaaag tagttttttt gcagaatatt tagcaagcaa agcaggagct aatccttatg
     3901 aatataaaaa tcctgaattt agaattaaag atattaatag taaagcaagt gattattcat
     3961 tcggatgtta tgataaaaaa aataatataa atttaataaa aatgtctgct gtaggtgata
     4021 tgtggggcac aggattattt aaatcaaatg cttgtgattt atgtgatgat gtaacaacag
     4081 aactagccga tatatcagtt ggagatgcat ggatgaaacc tttctctgat gatgggaaag
     4141 gaaataatgt cataattaca agatcggaat tagcaaatga cattattact tacgggataa
     4201 aaaataaaga gttaaacatt actaaattgt caacagatga atttttaaaa tctcagcaag
     4261 gtagttttac tcatagacat aatgccctac cttttcgtat aagaaggttt aaaaaattag
     4321 gaattttaat tccccccaaa agaatattta attcaagaat agatattcta gagagattaa
     4381 tacaaagaat gcgaatgaaa accagaagaa aaagtctcga actatggtta aaagaaaaaa
     4441 atcatataac atttgataaa aaaatgaaac ttgatctaac catattgaaa atattaacaa
     4501 aattaaatca tcttaaaaga agattaataa ttaaaatgaa agggtagtct atgaaaatag
     4561 gtattttaac tcaaccatta gttactaatt atggtggatt attacaagct tatgctttac
     4621 aaaaatatct gataaaaaat gattttgagg tattaacagt tgatattcgt cctaaaaaac
     4681 caaaattatg gggaataaaa agtataatag taaatataat taaaaaatat ctacttagga
     4741 gaaaaaatat aactattctt cctatatcta aaaaagaaaa aaaaacaatt cgaaaatata
     4801 cttctaaatt tataaatgaa aatatcaaaa caactgaaaa aataaaatca ataaaagaga
     4861 taaataaact taataagtat aattttgatg cttatatagt tggtagtgat caagtctggc
     4921 gtcctgcata ctcacctaat ataacaactt attttttaga ttttttaaaa aacgatataa
     4981 aaacaattcg tattgcttat gcagcttctt ttggattaga taattgtaat gagtttactc
     5041 aaaaacaatt aaaaatttgt tctgaattag caaagaaatt tcatttaata tcagtcaggg
     5101 aagattctgg tgttgaatta tgcaaaaaat attttaacct aaatgctcaa catgttttag
     5161 accctacaat gttattaaac aaagaggatt atattgagtt aataaaaaaa gataatatat
     5221 ctagaaacac aggcaatatg atgacatata ttttagattc atcaccagat aaagaaaaaa
     5281 taattaatat tgtagctaaa aaatataaat taactccgta ttcggtaatg cctgataaaa
     5341 ataacaaagt atatcctcct gttacacaat ggttgcgtgg tttttgggat gctgaatatg
     5401 ttgtaactga ctcatttcat ggtgttgtat tttcgattat ttttaataaa cagtttatag
     5461 ctataggtaa tataaaccgt ggattagctc gtttcagttc tattttaaaa aaatttagct
     5521 tagaaaatag aatcattttt tcttctaaag atttaaatga tgagtttttt aatcataaaa
     5581 tagattattg tttaattaat gaaatattag aaaaagaaaa aaacatagca tctttaactt
     5641 taaagaaagc acttaatact aataaatagg cctttccaac tatgatatct atcattattc
     5701 catgctataa tgattggcaa tcgttagaaa aaaatttaga ttctttacta ctatttgcaa
     5761 aaaaaatcga tggtgaagtt atcattatta atgatggtag ctccgataat atagctagag
     5821 atataaaaaa tataattaat cctcagttaa cttatcttga acaagaaaat aaaggtgttt
     5881 cgtcagccag aaataaaggg atcttatttg caaaaaaaga aaaactttta tttttagata
     5941 gcgacgatta tataaatttt gatgagttaa ttaaacattc ggaagaaata aaaaattttg
     6001 atttaatata ttgggacaca ataaaagtat ttccaaataa taagaatata atttacaaga
     6061 catatttaga aaaagacgta aatgatttag tttatgcatt actatgtaga aaatattatt
     6121 tattcatggg gagctttata atttcaaaga aaatttctca aaaaataatg tttgatgaaa
     6181 actataagta tggagaagat ttgaaattca tatatgaatg tatagacagc attaatagtt
     6241 ttactaaatt ggataatatt aatttatttt atattcaaag atgcaattcg gctatgtata
     6301 cattttctca tagaagattt gatagcgtta aagctttaga aagtatttct ataagaaaca
     6361 atacgctaaa aaaaattgat ttagaaaact tcataaaaaa ggataaaaaa ataattctta
     6421 ataattttat tcgctctttt cctttaggga aaatttattc taagagaaaa gaatttattg
     6481 aaataaataa tataattaaa aacatttttg gaaaaaacaa atttatttat gaactaaatt
     6541 tcttcctata cttaattatc tataaatttt atttgctaaa aaaataatat ggcaatatac
     6601 attatattta taataacaaa tttaattttg ttatctatac catttagatc taataacaat
     6661 atttattatc ctttaagttt tatatcttat atattaatat tatcattctc tggatttagg
     6721 tatggaatag gtactgacta ttttaattat gaaagtatat tttataacat tcattatgat
     6781 aatagttata atagcggact agaatatgga ttctactatt tatcaaaatt attaccacaa
     6841 gaaaaagaag gcttttttag tcttattttc ataacttctc taataacaaa tttcacttta
     6901 tttttagcca taaaaaatag aattaagaat aaatcacctg aagaaaggtt tcttataatt
     6961 ttattctttt ttattagtaa cttatatttt ttgccattta atggtatcag gcagggaatc
     7021 gctttctctt gtactttatt atcatttacg ttcataaaac aaagacgttt tctttatttc
     7081 ctaatattaa ttttaatagg tagtttattt cacaaatcaa tattattatt tattccgttt
     7141 tattttgtat ataaaataaa tattaaaaac ttgtattgct ttattattgt ctccatttct
     7201 tttattttag taaagtcagg tatatttagt ttaacaataa atagtttgat taacattctt
     7261 ggtgaaaaat atgccagata tttggaaaat cctgttgact atggaggttc tgggcttggt
     7321 gtttatttat acgttatatt attcctattt atttatttta taaaatttgt cgtcagaaat
     7381 gagaaaatga aaaccaaaga atataatttt ttatttctta tgttttctat aggcatttca
     7441 tttagaatac tagcattaga taacattata tttgtcaggc catcatatta cttcaccgtt
     7501 tttgatactt ttttcattcc tttctttatt atgtgcttta ataagaataa atttattatt
     7561 agtattgtaa ttacttgttt ctatctaata agtttcttgg cttcattatc aaattcaaat
     7621 gggcttttac cttaccagaa tatatttata agctaggtta aaaatgaaaa aaattgctat
     7681 cttagtacct aatctaaaag ataatggagt tagtagagtt gcagctctac attccatcat
     7741 gttttcaaat caaggttatc tcgttgatat aatcgtagaa gaaaataaat ctgttaaatt
     7801 cccattttca ggaaacctaa taaatttaaa tttaaaaaga aaacgggggg tatttaaaat
     7861 acttaacttt attaagttat atttaaaaat aaaaaaaata aaaacttcta ataaatatga
     7921 tttttttatt agtcatattc ctcattgtga tattataaat tctataacaa aaacaaaaaa
     7981 caaagaaatt acgatatcga cagtacataa taacgttgaa ctaagatact ctaaaataac
     8041 aaaattacta ttaaaaataa ttattttaaa aaaatcagat gccgttgtca ctgtttctga
     8101 agatttagct tctcagctta aaaaaaaata ttcaaattat aataaaataa taaacattcc
     8161 taatcctgtt gactttaatt taataaaaaa atcattaaac gctcagttac cagaatatct
     8221 aagcaataaa aaatttatat taaatattgg tcgattagat tcacaaaaag gtcactggca
     8281 tttaataaaa gcattttatt taacattaaa aaagcaacct gatttatatt tagtaattat
     8341 tggtgaagga ccagagaaaa taatattaaa aaatctatgc aaaagtttaa atatagataa
     8401 aaaagtaatc ttcgagggtt ttaaagaaaa tcctttcata tatatgaata aatcatcagc
     8461 ttttgttttc ccttctttat acgaaggcct tcctatggta ttaatagaag caatggtttg
     8521 tagaactcct attatttcta gtgactgttt ttccggcccc agagagcttt taattaaaga
     8581 taatattacc tatgggaaat taataccaaa ttatggaact acttcttcat tatatactaa
     8641 atctataggt gattacgata gattgatttc aaatgaaata ttgcttttat tgaattgtgt
     8701 agctaataaa aaaaatttag atttagctga aaatagagct aaagagttta atttagaaaa
     8761 tattttttta agatggaagg atttatttga tgagtacatc taaaaatttt agaaataaat
     8821 acctttttca tagaatatta cattttataa aaggaatttt gttttcaata tattattcat
     8881 atatatatac aaaaagaaaa aaaactatta tatttaattc tgcatgtaat caagagttta
     8941 catggaatgc taaaagttta tttcttcatt ctagaaataa acttgaagct ctaggtttta
     9001 aagtatattt tgttatcaat gataaaaata aaagagaata tctaacatct ctttatggca
     9061 actattttat atgcaataag aaaaaaacca atgtcattaa aatatatact gcggctatct
     9121 ggattttaag tacattagaa acaccttgtt caggaatttt cttaaatagg aaaagatttg
     9181 tctatcattt aggtcatgga acgccaataa aaaatatagg attatgtgaa aataatttag
     9241 gtttcttaaa aaaatctttt tatagattaa acaagagtaa tatttcatta tttttaagct
     9301 cttcacattt ttttaaaaaa tatatggcta gagcatttgg tgttaatgaa gaaaagattg
     9361 ttgtggcacc acagcctaga attgaagatt taataaataa aaataaaaac ataaactttt
     9421 cttttcaaaa ggataaaaaa tatatacttt atgctcctac ttggcgtcat tatgacgatg
     9481 ttaaactatt tccttacatt aaagataata tcgataattt gaatcgagaa cttcaacaac
     9541 ttaacataat tattttattg agattacatc ctagatttga gtctaattta aatagttacc
     9601 taagaactaa tattttaaat tttaattcaa aaatttgtga agatatctct gatgctctat
     9661 attttacctc tggtcttata actgattatt ctagtattta ctgcgattat ttattattaa
     9721 aaaaaccagt tgcattgtta ccttatgatt tagataaata tgttaaggaa ataggttttt
     9781 catttctata taaagaaatt ttccctaaaa ccataataaa aaatcatact gactttatcg
     9841 atttttgtgc acaagtgtct aatgattgtt ttaatatcga agaacaaaat actttagcag
     9901 aaaaattaaa ttttatccca gataattgtt caatcacaga ctacaatata tctatcatta
     9961 tgaaggagta taaaaaaaga tggccgtcag aataataact tttggaacat ttgatgtctt
    10021 tcatataggg catataaaaa tacttgaaag agctcgaagc ttaggtgatt atttaattgt
    10081 aggggtatcg tctgataagt taaacttcaa gaaaaaaaac agatacccta tatataatca
    10141 aaacgataga attgatatta tatcatcatt aaaatatgtc gatgaagtat tcattgaaga
    10201 atctttagaa ttaaaagaat attatattaa aagctttaat gctgatattc tagtgatggg
    10261 aaatgattgg gagggaaaat ttgatcattt aggaaaaata tgtaaagtta tctatttacc
    10321 tagaacaccg tcaatatcaa ctacagaaat tattgaaatc gctaaagcaa gataagtatt
    10381 actcattcct ttctattgct taacgtatat tgcaatatat tccatatggt tttaatatat
    10441 tatgaaaatc accatctcag gaaccggcta tgtcggtcta tctaacggga tactcctttc
    10501 ccagcacaac aaagttgttg cattagatat caataaagaa aaagtcgaac tattaaacca
    10561 aaaaatctct cctatctctg ataaagaaat tgaagagttt ttagccacta aagagctcga
    10621 ctttacggca actttagata aagaattcgc ttataaagat gctgaatttg ttatcattgc
    10681 cactcctact gattatgatc ctaaaactaa ctactttaac acctcttctg tagagtcggt
    10741 tattcgtgat gttttagaat ataatccaaa caccactatg attataaaat caaccattcc
    10801 agttggtttt actaaacaga tgagagaaaa atatcatacc gataatatta tcttctctcc
    10861 tgagttctta cgtgaaggtc gcgcccttta cgataacctt tatccgtctc gtattgtgat
    10921 tggtgagcaa tctgcaagag cagagaaatt tgctgagctt ttacaacaag gggcattaaa
    10981 aaaagatatt gatgttcttt ttactgattc tacagaagca gaagccatta agttatttgc
    11041 taatacttat ctcgcactgc gtgttgctta ttttaatgaa ctcgatagct acgcccaagc
    11101 ttacggtcta aacgcaagac aaattattga aggtgtttgt ttagatccgc gtattggtaa
    11161 tcactataac aacccatcat ttggttatgg cggttactgc ttaccaaaag atactaaaca
    11221 actgttagcc aactacgaat cagtgcctaa taatattatt ggcgccattg tggaagctaa
    11281 ccgcactcgt aaagacttta ttgctgattc tattatcgct aaatcaccga agattgttgg
    11341 cgtttatcgt ttaattatga aatcaggctc tgataatttc cgttcatctt caatccaagg
    11401 gattatgaag cgtattaaag cgaaagggat tgaagtggtg atttatgagc cagaaatgaa
    11461 agaagagact ttctttaact caaaattaat tcataattta gatgagttta aagcaatgtc
    11521 tgatgtcatt attaccaata gaatggcttc tgcactaaac gatgttgaag aaaaagttta
    11581 tactcgtgat ctctttggta acgattaaaa ctaatttata tgctcaatat cgtcttattt
    11641 gaacccgaaa ttccaccgaa tacgggtaat attatccgcc tgtgtgccaa cacaggcttt
    11701 catcttcatt tgatccaacc attgggtttt acttgggatg ataaacggct acgccgtgca
    11761 gggcttgatt atcgtgagtt tgctgatatc aaacagcatc acgattatta tgctttttta
    11821 gcaagtgaag gattaagcac tgataatgca caatccccga caggtgcgcg gttgtttgca
    11881 ttaaccacta aaggtacccc cgcccatagt aatgtaagtt atcacgatgg ggattatctg
    11941 ctatttggcc ctgagacacg aggtttacca ccttatgtgt tggataatat gccaatacaa
    12001 caaaaaatcc gtgtgccaat gttagcagat agccgcagta tgaatctttc taatacggtt
    12061 gccgtggttg tttttgaggc atggcgtcaa ttaggctatc cgggggcatt attaagagat
    12121 taatttatat ttttaattta taaccataaa aaatgacctc tagacttcat tgtctcgagg
    12181 tcatttttat gtatatgctt tgtgctagtt ttagcttttc ttgttttata tcttctttgt
    12241 atctttttta tatctttcgg ttttatttct tagctttttt agttattttt cttatcaaga
    12301 aataccatct tgcacttact tttattaaaa atgatgagaa atcagttact aatatctttc
    12361 tttctaataa tcttttctta aattcatttt tttgtattaa ctcttttcga tactctttag
    12421 cattaaattg ttggttaatc ttttcaagta accttttaca gcgtactact tgctctactg
    12481 tttttttatc attatccaat aataatgaat aatcctcttt attcacgtta atgccaaaat
    12541 acattaataa tttttgccct actctttccg cggtatcttt ttgtatcgct ttatattgcg
    12601 agcgatcgac atttggatga gtacgataat aaagaagtgg tacaggaaga ttatggagct
    12661 taaattttcc agcaataatt aagttcaccc ataaaccata atcttctgca tagaggtctg
    12721 ctttatcata tccaccaaca gataatatat cttgcttacg atagataatg gttggatgcg
    12781 ccaaggagca atcataaaga ctagaaatca caatatcact atgtgtttct ggataagtat
    12841 taattctatt tggctcttct tggtagataa aagcaccacc acaaacactc acttcaggat
    12901 gttgtaacat aaattgatat tgcgcttcta gtctggttga aacggcaata tcatcagagt
    12961 ccattctagc aactaaatca catgaagcta attgtaatcc tttgtttaaa gaagcgatca
    13021 atccgatatt ttgttcatta ataatcagtt ttattctggg atcttttttg gcattttctt
    13081 ttaagataga taatgagtta tccgttgagc catcattaat gataagaaac tcaaattctt
    13141 taaatgtttg atttaatata gaatcaatag actcctgaag gtatttctca tcattgtata
    13201 ccgacattaa tacggtaatt ttgggcatat tgtttttcat tatctatttt cagcaatatt
    13261 tattaatttc aaaattcatc ttattgatat accgacaccc ttctcccaca tcctttaata
    13321 ctaatgcatg ggcgccaata atcgcattat caccaatctt aatctcacca ccaataatgg
    13381 acgaattaca gccaatataa acatggtttc ctacttggat attggctttt ccccctttct
    13441 cttttacgcc aatagtcacc ccttgcataa tagtgacata ttcccccagt tgacagtaat
    13501 tagtcagtac aatgccaatc gggtgtggta aagaaaggcc tttaccaata cgggttttta
    13561 acgagatatc attacagtac ttacgattat tgctagcacc gatccgaata cctaatttat
    13621 gtaaaaagcg atggggagag aaataaaaat actgtgctac ccgccaccaa aataggtaac
    13681 gggaacgctg gcagaaaaat actcgatgaa atagtcttaa ccaactaaag ggttttttat
    13741 caccaatata ttcagcctgt aagcaatctt ttagttcagc aagggtaacc ttagcttgag
    13801 catgcatagt taaataccat caccacactc aaagccgggt acaacaccat taaagtgttg
    13861 atccatatcg aatgacggtt tttcactatc aggacgaccg acaatgcggg caggcacccc
    13921 cgccaccgta gtatgagccg gtacttcatg taatactacc gagccagcac caattttagc
    13981 accacgacca atctcgatat taccaagaat tttagctcct gcgccaatca ttaccccttc
    14041 acgcactttc gggtgacgat cgcctcctgt tttacctgtt ccccctaagg tcacagattg
    14101 taaaatggag acatcatttt caaccaccgc cgtttcacca atcacaatac ctgtcgcatg
    14161 gtcgagcata ataccgtgtc caatacgtgc cgcagggtga atatccaccc caaaggtgac
    14221 tgatatttgg ttttgtagat aaatggctag cgcttggcga ttttgttgcc atagccaatg
    14281 ggcaatacgg taggcttgta atgcatgaaa cccttttaaa tagagcaatg gggttgaata
    14341 tttatctact gcgggatcac gtaggcgtac cgcggagaga tcacaagcgg ccgactcaat
    14401 cattgagcta tcgtgactat aagcctcttc aacaatttct cgtactgcaa tggctggcat
    14461 agtctgagtt gccagcttat tggccagcat atagcttaat gcactaccta aattttcgtg
    14521 tttgagaagt gtcgcatgaa agaaactggc taacattggc tcacaatctg ccaatgcacg
    14581 cgcttcgctt ttaatatgat cccagactct tgttagttct tctagcgaca tactcactct
    14641 cattttttac accaaagcct aagtaaatac ttaggctttg aattttcaaa caaatggata
    14701 gttaaaacag ataccctaag aatattattt aaaataaact tacaacaggc tgctgtctat
    14761 ctcatctttg gttgctcgcc ccaataaagc caatgcagcc tcttttacat ctttatgttg
    14821 atataaaatt tggtaaatct gttcggtgat tggcatctct acacccactt gttcggctaa
    14881 tgcgcgaact tcttcggtgt tacgataacc ttcgactacc tggccaattt tctcttgggc
    14941 tgtatcaaca tctaaacctt gacctagcat cataccaaag cgacgattac gtgattggtt
    15001 gtcagtacag gttaaaacta aatcacccaa accagccatg cccataaaag ttgccgcatc
    15061 tgcacctaag gctttaccta agcggctcat ttcggccaga ccacgggtaa taagtgcagt
    15121 acgcgcatta gcgccaaatc ccataccatc agacataccc gcaccaatag caatcacgtt
    15181 tttaacagcg ccccccagtt gcacaccgat aaaatcaggg tttttataga ctcggaagct
    15241 tttaccacaa tgaaatagct gttgtaactg ctctaaaaag agattgtctg tcgaggccac
    15301 tgcaatcgcg gtgggcaaac ccgcggcaag ctctttagca aaagtagggc cagataatac
    15361 tgcgagcggg atttcattac ctaatacttc acgggcaaca tcttgtaata agcgaccagt
    15421 atgtgcttca agaccttttg tcgcccaaac aacacgtgca tcctgacgta aaaagggttt
    15481 gatttgttgc agtacttcac caaacacatg gcttgggatc accacaagaa tattacggct
    15541 cgcttcaatc gctttttgca aagaagcttc catatataaa ctatcaggaa aggaaacatc
    15601 gggcagaaag gcttgattac agcgcgcttg ttctaatgcc gcaacgtgct tgggatcatg
    15661 cccccacagc acaacatcgt gcccattacg cgctaaggta atcgctaaag cggtgccgta
    15721 tgaaccggca ccgataactg tcatagaagc gttcattaag cttcttcaac tcctgagttt
    15781 gctgcatcct gttgctgttg ttgctgtaag tagttcataa acaacgcatc aaagttaaca
    15841 ggtgctaagt ttaattgtgg gaaagtacca cgacccacta agttggtgat acattcacgt
    15901 gcataaggga ataatacgtt cgggcagtat gcacctaagc aatgtgctaa ttgtgtacct
    15961 tcgatacctt caacagtaaa gatacctgct tgttgtactt cacacaggaa agcg
//
